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Pesquisa : D08.244.453.005.550 [Categoria DeCS]
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Id: lil-797893
Autor: Da Costa, N Meireles; Visoni, SBC; Dos Santos, IL; Barja-Fidalgo, TC; Ribeiro-Pinto, LF.
Título: Maternal protein restriction during lactation modulated the expression and activity of rat offspring hepatic CYP1A1, CYP1A2, CYP2B1, CYP2B2, and CYP2E1 during development
Fonte: Braz. j. med. biol. res = Rev. bras. pesqui. méd. biol;49(11):e5238, 2016. graf.
Idioma: en.
Resumo: Early nutrition plays a long-term role in the predisposition to chronic diseases and influences the metabolism of several drugs. This may happen through cytochromes P450 (CYPs) regulation, which are the main enzymes responsible for the metabolism of xenobiotics. Here, we analyzed the effects of maternal protein restriction (MPR) on the expression and activity of hepatic offspring’s CYPs during 90 days after birth, using Wistar rats as a mammal model. Hepatic CYP1A1, CYP1A2, CYP2B1, CYP2B2 and CYP2E1 mRNA and protein expression, and associated catalytic activities (ECOD, EROD, MROD, BROD, PROD and PNPH) were evaluated in 15-, 30-, 60-, and 90-day-old offspring from dams fed with either a 0% protein (MPR groups) or a standard diet (C groups) during the 10 first days of lactation. Results showed that most CYP genes were induced in 60- and 90-day-old MPR offspring. The inductions detected in MPR60 and MPR90 were of 5.0- and 2.0-fold (CYP1A2), 3.7- and 2.0-fold (CYP2B2) and 9.8- and 5.8– fold (CYP2E1), respectively, and a 3.8-fold increase of CYP2B1 in MPR90. No major alterations were detected in CYP protein expression. The most relevant CYP catalytic activities’ alterations were observed in EROD, BROD and PNPH. Nevertheless, they did not follow the same pattern observed for mRNA expression, except for an induction of EROD in MPR90 (3.5-fold) and of PNPH in MPR60 (2.2-fold). Together, these results suggest that MPR during lactation was capable of altering the expression and activity of the hepatic CYP enzymes evaluated in the offspring along development.
Descritores: Sistema Enzimático do Citocromo P-450/metabolismo
Dieta com Restrição de Proteínas
Lactação/metabolismo
Fígado/enzimologia
-Hidrocarboneto de Aril Hidroxilases/metabolismo
Citocromo P-450 CYP1A1/metabolismo
Citocromo P-450 CYP1A2/metabolismo
Citocromo P-450 CYP2B1/metabolismo
Citocromo P-450 CYP2E1/metabolismo
Modelos Animais
Ratos Wistar
Esteroide Hidroxilases/metabolismo
Fatores de Tempo
Limites: Animais
Feminino
Ratos
Responsável: BR1.1 - BIREME


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Id: lil-252263
Autor: Kuriyama, S. N; Oliveira, A. C. A. X de; Fidalgo-Neto, A. A; Paumgartten, F. J. R.
Título: Effects of pregnancy and protein-energy malnutrition on monooxygenase O-dealkylation activity in rat liver microsomes
Fonte: Braz. j. med. biol. res = Rev. bras. pesqui. méd. biol;33(1):103-9, Jan. 2000. tab.
Idioma: en.
Resumo: Xenobiotic metabolism is influenced by a variety of physiological and environmental factors including pregnancy and nutritional status of the individual. Pregnancy has generally been reported to cause a depression of hepatic monooxygenase activities. Low-protein diets and protein-energy malnutrition have also been associated with a reduced activity of monooxygenases in nonpregnant animals. We investigated the combined effects of pregnancy and protein-energy malnutrition on liver monooxygenase O-dealkylation activity. On pregnancy day 0 rats were assigned at random to a group fed ad libitum (well-nourished, WN) or to a malnourished group (MN) which received half of the WN food intake (12 g/day). WN and MN rats were killed on days 0 (nonpregnant), 11 or 20 of pregnancy and ethoxy- (EROD), methoxy- (MROD) and penthoxy- (PROD) resorufin O-dealkylation activities were measured in liver microsomes. Only minor changes in enzyme activities were observed on pregnancy day 11, but a clear-cut reduction of monooxygenase activities (pmol resorufin min-1 mg protein-1) was noted near term (day 0 vs 20, means + or _ SD, Student t-test, P<0.05) in WN (EROD: 78.9 + or - 15.1 vs 54.6 + or - 10.2; MROD: 67.8 + or - 10.0 vs 40.9 + or - 7.2; PROD: 6.6 + or - 0.9 vs 4.3 + or - 0.8) and in MN (EROD: 89.2 + or - 23.9 vs 46.9 + or - 15.0; MROD: 66.8 + or - 13.8 vs 27.9 + or - 4.4; PROD: 6.3 + or - 1.0 vs 4.1 + or - 0.6) dams. On pregnancy day 20 MROD was lower in MN than in WN dams. Malnutrition did not increase the pregnancy-induced reduction of EROD and PROD activities. Thus, the present results suggest that the activities of liver monooxygenases are reduced in near-term pregnancy and that protein-energy malnutrition does not alter EROD or PROD in pregnant rats
Descritores: Citocromo P-450 CYP1A1/metabolismo
Citocromo P-450 CYP2B1/metabolismo
Microssomos Hepáticos/enzimologia
Complicações na Gravidez
Desnutrição Proteico-Calórica/enzimologia
-Análise de Variância
Biotransformação
Tamanho do Órgão
Ratos Wistar
Ganho de Peso
Xenobióticos/metabolismo
Limites: Ratos
Animais
Feminino
Gravidez
Responsável: BR1.1 - BIREME



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