Base de dados : LILACS
Pesquisa : D08.811 [Categoria DeCS]
Referências encontradas : 480 [refinar]
Mostrando: 1 .. 10   no formato [Detalhado]

página 1 de 48 ir para página                         

  1 / 480 LILACS  
              next record last record
seleciona
para imprimir
Fotocópia
Texto completo SciELO Brasil
Romano, Luis A
Texto completo
Id: biblio-886641
Autor: RAMOS, LEONARDO R V; PEDROSA, VIRGÍNIA F; MORI, AGNES; ANDRADE, CARLOS F F DE; ROMANO, LUIS A; ABREU, PAULO C; TESSER, MARCELO B.
Título: Exogenous enzyme complex prevents intestinal soybean meal-induced enteritis in Mugil liza (Valenciennes, 1836) juvenile
Fonte: An. acad. bras. ciênc;89(1):341-353, Jan,-Mar. 2017. tab, graf.
Idioma: en.
Resumo: ABSTRACT Four soybean meal-based diets containing increasing levels of an enzyme complex (E50, E100, E150 and E200 at 50, 100, 150 and 200 g ton-1, respectively) and one soybean meal-based diet without the enzyme complex (E0) were fed in triplicate to M. liza juveniles in a semi-static flow system with 20 fish per tank for 75 days. There were no differences between the treatments for animal performance parameters, but fish fed the enzyme complex treatment exhibited significantly (P<0.05) higher values of calcium bone retention compared with control fish. Although there was no relationship between bacterial counts in different sections of the gastrointestinal tract or enzyme levels, filamentous bacteria were increased in E50 compared with E150. All of the treatments resulted in higher bacterial counts in the stomach than in intestinal segments. Histological screening showed serious to moderate infiltration of inflammatory cells, modification in villus morphology and necrosis in some cases in fish fed the E0 diet. In addition, fish from the E0 treatment exhibited significantly (P<0.05) lower lipid deposition in the peritoneal cavity. Therefore, the use of low levels of exogenous enzyme is recommended in diets for M. liza when soybean meal is used as the main source of protein.
Descritores: Soja
Smegmamorpha/crescimento & desenvolvimento
Dieta/veterinária
Enterite/veterinária
Enzimas/administração & dosagem
Doenças dos Peixes/prevenção & controle
-Valores de Referência
Fatores de Tempo
Reprodutibilidade dos Testes
Análise de Variância
Estatísticas não Paramétricas
Smegmamorpha/microbiologia
Trato Gastrointestinal/microbiologia
Trato Gastrointestinal/patologia
Enterite/microbiologia
Enterite/patologia
Enterite/prevenção & controle
Carga Bacteriana
Ração Animal/análise
Limites: Animais
Tipo de Publ: Estudo de Avaliação
Responsável: BR1.1 - BIREME


  2 / 480 LILACS  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo SciELO Brasil
Texto completo
Id: biblio-886937
Autor: MARQUES NETTO, CATERINA G C; PALMEIRA, DAYVSON J; BRONDANI, PATRÍCIA B; ANDRADE, LEANDRO H.
Título: Enzymatic reactions involving the heteroatoms from organic substrates
Fonte: An. acad. bras. ciênc;90(1,supl.1):943-992, 2018. tab, graf.
Idioma: en.
Resumo: ABSTRACT Several enzymatic reactions of heteroatom-containing compounds have been explored as unnatural substrates. Considerable advances related to the search for efficient enzymatic systems able to support a broader substrate scope with high catalytic performance are described in the literature. These reports include mainly native and mutated enzymes and whole cells biocatalysis. Herein, we describe the historical background along with the progress of biocatalyzed reactions involving the heteroatom(S, Se, B, P and Si) from hetero-organic substrates.
Descritores: Bactérias/metabolismo
Biotransformação
Enzimas/metabolismo
Biocatálise
Fungos/metabolismo
-Especificidade por Substrato
Técnicas Biossensoriais
Enzimas/química
Tipo de Publ: Revisão
Responsável: BR1.1 - BIREME


  3 / 480 LILACS  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo SciELO Brasil
Texto completo
Id: biblio-886938
Autor: PEREIRA, ANDRESSA R; SEDENHO, GRAZIELA C; SOUZA, JOÃO C P DE; CRESPILHO, FRANK N.
Título: Advances in enzyme bioelectrochemistry
Fonte: An. acad. bras. ciênc;90(1,supl.1):825-857, 2018. tab, graf.
Idioma: en.
Projeto: Fundação de Amparo à Pesquisa do Estado de São Paulo; . Conselho Nacional de Desenvolvimento Científico e Tecnológico; . FAPESP.
Resumo: ABSTRACT Bioelectrochemistry can be defined as a branch of Chemical Science concerned with electron-proton transfer and transport involving biomolecules, as well as electrode reactions of redox enzymes. The bioelectrochemical reactions and system have direct impact in biotechnological development, in medical devices designing, in the behavior of DNA-protein complexes, in green-energy and bioenergy concepts, and make it possible an understanding of metabolism of all living organisms (e.g. humans) where biomolecules are integral to health and proper functioning. In the last years, many researchers have dedicated itself to study different redox enzymes by using electrochemistry, aiming to understand their mechanisms and to develop promising bioanodes and biocathodes for biofuel cells as well as to develop biosensors and implantable bioelectronics devices. Inside this scope, this review try to introduce and contemplate some relevant topics for enzyme bioelectrochemistry, such as the immobilization of the enzymes at electrode surfaces, the electron transfer, the bioelectrocatalysis, and new techniques conjugated with electrochemistry vising understand the kinetics and thermodynamics of redox proteins. Furthermore, examples of recent approaches in designing biosensors and biofuel developed are presented.
Descritores: Fontes de Energia Bioelétrica
Técnicas Biossensoriais
Eletroquímica
Transporte de Elétrons
Enzimas/química
-Enzimas/fisiologia
Responsável: BR1.1 - BIREME


  4 / 480 LILACS  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo SciELO Cuba
Texto completo
Id: biblio-1089725
Autor: Hernández Ortega, Rudy; González Alfonso, Osvaldo; Cabrera Pratts, Antonio J; Cruz Boza, Raúl; Suárez López, Juliette M; Bulies de Armas, Gilberto; Sánchez Hernández, Yahima.
Título: Protección renal y hepática por condicionamiento isquémico a distancia en la revascularización miocárdica quirúrgica / Renal and hepatic protection due to remote ischemic conditioning in coronary artery bypass grafting
Fonte: CorSalud;11(2):139-145, abr.-jun. 2019. graf.
Idioma: es.
Resumo: RESUMEN Introducción: Breves períodos de isquemia a distancia pueden limitar el daño miocárdico producido por la isquemia/reperfusión. Objetivos: Identificar el efecto del condicionamiento isquémico a distancia con fines de protección renal y hepática, relacionado al comportamiento postoperatorio de los niveles de creatinina y transaminasas glutámico-purúvica y glutámico-oxalacética en la revascularización miocárdica quirúrgica. Método: Se realizó un estudio cuasiexperimental, explicativo, comparativo con control histórico, en dos grupos de 247 pacientes cada uno, propuestos para revascularización miocárdica quirúrgica. Se colocó un torniquete en el brazo derecho, en el grupo estudio, alternando 3 insuflaciones (con una presión de 200 mmHg) con 3 desinsuflaciones, durante cinco minutos cada una. Este procedimiento se realizó previo, durante y después de la mayor isquemia inducida, provocada por el pinzamiento de la arteria coronaria. Resultados: Se logró una disminución significativa en los valores de creatinina (p<0,001), transaminasa glutámico-purúvica (p<0,001) y transaminasa glutámico-oxalacética (p<0,05). Conclusiones: El condicionamiento isquémico a distancia es una importante herramienta a tener en cuenta para la protección renal y hepática en la revascularización miocárdica quirúrgica.

ABSTRACT Introduction: Short periods of distant ischemia can limit myocardial damage caused by ischemia/reperfusion. Objective: To identify the effect of remote ischemic preconditioning in relation to the postoperative behavior of creatinine, glutamic transaminase, puruvic and oxalacetic levels. Method: A quasi-experimental, explanatory, comparative study with historical control was carried out in two groups of 247 patients each; all candidates for coronary artery bypass grafting. A blood-pressure cuff was placed on the right arm in the study group alternating three inflations with three deflations of five minutes at 200 mmHg. This procedure was performed prior to during and after the major ischemic episode caused by the coronary artery impingement. Results: A significant decrease in the values ​​of creatinine, puruvic glutamic transaminase and glutamic oxalacetic transaminase was achieved. Conclusions: Remote ischemic conditioning is an important tool to take into account for renal and hepatic protection in coronary artery bypass grafting.
Descritores: Precondicionamento Isquêmico
-Traumatismo por Reperfusão
Creatinina
Enzimas
Transaminases
Revascularização Miocárdica
Responsável: CU425.1 - Centro Provincial de Información de Ciencias Médicas de Villa Clara


  5 / 480 LILACS  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
Id: biblio-1053462
Autor: Panyachanakul, Titiporn; Sorachart, Bodeesorn; Lumyong, Saisamorn; Lorliam, Wanlapa; Kitpreechavanich, Vichien; Krajangsang, Sukhumaporn.
Título: Development of biodegradation process for Poly(DL-lactic acid) degradation by crude enzyme produced by Actinomadura keratinilytica strain T16-1
Fonte: Electron. j. biotechnol;40:52-57, July. 2019. graf, tab.
Idioma: en.
Projeto: Srinakharinwirot University research fund, Thailand; . Center of Excellence on Biodiversity (BDC), office of higher education commission, Thailand.
Resumo: Background: Plastic waste is a serious problem because it is difficult to degrade, thereby leading to global environment problems. Poly(lactic acid) (PLA) is a biodegradable aliphatic polyester derived from renewable resources, and it can be degraded by various enzymes produced by microorganisms. This study focused on the scale-up and evaluated the bioprocess of PLA degradation by a crude microbial enzyme produced by Actinomadura keratinilytica strain T16-1 in a 5 L stirred tank bioreactor. Results: PLA degradation after 72 h in a 5 L bioreactor by using the enzyme of the strain T16-1 under controlled pH conditions resulted in lactic acid titers (mg/L) of 16,651 mg/L and a conversion efficiency of 89% at a controlled pH of 8.0. However, the PLA degradation process inadvertently produced lactic acid as a potential inhibitor, as shown in our experiments at various concentrations of lactic acid. Therefore, the dialysis method was performed to reduce the concentration of lactic acid. The experiment with a dialysis bag achieved PLA degradation by weight loss of 99.93%, whereas the one without dialysis achieved a degradation of less than approximately 14.75%. Therefore, the dialysis method was applied to degrade a commercial PLA material (tray) with a conversion efficiency of 32%, which was 6-fold more than that without dialysis. Conclusions: This is the first report demonstrating the scale-up of PLA degradation in a 5 L bioreactor and evaluating a potential method for enhancing PLA degradation efficiency.
Descritores: Poliésteres/metabolismo
Actinomycetales/enzimologia
Enzimas/metabolismo
-Polímeros/metabolismo
Biodegradação Ambiental
Ácido Láctico/análise
Reatores Biológicos
Concentração de Íons de Hidrogênio
Responsável: CL1.1 - Biblioteca Central


  6 / 480 LILACS  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
Id: biblio-1053493
Autor: Gao, Ling; Guo, Qingqing; Lin, Huibin; Pan, Deng; Huang, Xiaodong; Lin, Jianqun; Lin, Jianqiang.
Título: Modeling of lactose enzymatic hydrolysis using Monte Carlo method
Fonte: Electron. j. biotechnol;40:78-83, July. 2019. tab, graf, ilus.
Idioma: en.
Projeto: Taishan Industry Leading Talents Engineering for High-Efficiency Ecoagriculture Innovation project of Shandong province, China; . National Natural Science Foundation of China.
Resumo: Background: Mathematical modeling is useful in the analysis, prediction, and optimization of an enzymatic process. Unlike the conventional modeling methods, Monte Carlo method has special advantages in providing representations of the molecule's spatial distribution. However, thus far, Monte Carlo modeling of enzymatic system is namely based on unimolecular basis, not suitable for practical applications. In this research, Monte Carlo modeling is performed for enzymatic hydrolysis of lactose for the purpose of real-time applications. Results: The enzyme hydrolysis of lactose, which is conformed to Michaelis­Menten kinetics, is modeled using the Monte Carlo modeling method, and the simulation results prove that the model predicts the reaction kinetics very well. Conclusions: Monte Carlo modeling method can be used to model enzymatic reactions in a simple way for real-time applications.
Descritores: Método de Monte Carlo
Enzimas/metabolismo
Hidrólise
Lactose/metabolismo
-Fatores de Tempo
Cinética
beta-Galactosidase/metabolismo
Enzimas Imobilizadas
Galactose/metabolismo
Responsável: CL1.1 - Biblioteca Central


  7 / 480 LILACS  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
Id: biblio-1053552
Autor: Smuga-Kogut, Malgorzata; Piskier, Tomasz; Walendzik, Bartosz; Szymanowska-Powalowska, Daria.
Título: Assessment of wasteland derived biomass for bioethanol production
Fonte: Electron. j. biotechnol;41:1-8, sept. 2019. tab, ilus, graf.
Idioma: en.
Projeto: National Science Centre as part of MINIATURA 1.
Resumo: Background: The bioethanol produced from biomass is a promising alternative fuel. The lignocellulose from marginal areas or wasteland could be a promising raw material for bioethanol production because it is present in large quantities, is cheap, renewable and has favorable environmental properties. Despite these advantages, lignocellulosic biomass is much more difficult to process than cereal grains, due to the need for intensive pretreatment and relatively large amounts of cellulases for efficient hydrolysis. Therefore, there is a need to develop an efficient and cost-effective method for the degradation and fermentation of lignocellulosic biomass to ethanol. Results: The usefulness of lignocellulosic biomass from wasteland for the production of bioethanol using pretreatment with the aid of ionic liquids of 1-ethyl-3-methylimidazolium acetate and 1-ethyl-3-methylimidazolium chloride was evaluated in this study. The pretreatment process, enzymatic hydrolysis and alcoholic fermentation lasted a total of 10 d. The largest amounts of bioethanol were obtained from biomass originating from agricultural wasteland, in which the dominant plant was fireweed (Chamaenerion angustifolium) and from the field where the common broom (Cytisus scoparius) was the dominant. Conclusions: The plants such as fireweed, common broom, hay and goldenrod may be useful for the production of liquid biofuels and it would be necessary in the further stage of research to establish and optimize the conditions for the technology of ethyl alcohol producing from these plant species. Enzymatic hydrolysis of biomass from agricultural wastelands results in a large increase in fermentable sugars, comparable to the enzymatic hydrolysis of rye, wheat, rice or maize straw.
Descritores: Solo/química
Biomassa
Etanol/metabolismo
-Biodegradação Ambiental
Celulases/análise
Enzimas/metabolismo
Líquidos Iônicos
Biocombustíveis
Hidrólise
Lignina/análise
Responsável: CL1.1 - Biblioteca Central


  8 / 480 LILACS  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo SciELO Brasil
Texto completo
Id: lil-666064
Autor: Lourenço, Maria Lucia G; Takahira, Regina K; Machado, Luiz Henrique A; Moutinho, Flávio Q; Ferreira, Helena; Balieiro, Júlio César C; Machado, Luciana P; Fonteque, Joandes H.
Título: Monitoramento de parâmetros laboratoriais em gatos sem raça definida durante o período neonatal / Monitoring of laboratory parameters during neonatal period in mixed-bred cats
Fonte: Pesqui. vet. bras = Braz. j. vet. res;32(supl.1):25-31, Dec. 2012. ilus, tab.
Idioma: pt.
Resumo: O objetivo do presente estudo foi à monitoração dos parâmetros laboratoriais como hemograma, enzimas hepáticas alanina aminotransferase (ALT) e gama-glutamiltransferase (GGT), glicemia e proteinograma sérico, e avaliar o efeito da idade em gatos sem raça definida durante a fase neonatal. Vinte gatos machos e fêmeas foram utilizados a partir do terceiro dia de vida até o 38º dia de idade. As amostras de sangue foram colhidas semanalmente e as análises laboratoriais (hemograma, enzimas hepáticas, glicemia e proteinograma sérico) realizadas no 3º, 10º, 17º, 24º, 31º e 38º dia de idade. Os resultados exibiram efeito significativo da idade sobre a contagem total de eritrócitos, concentração de hemoglobina, volume globular, volume corpuscular médio, concentração de hemoglobina corpuscular média, leucócitos totais, neutrófilos, eosinófilos e basófilos. Nenhum efeito foi observado em células como linfócitos, monócitos ou na concentração sérica de glicose. A análise das modificações ocorridas nos parâmetros laboratoriais durante a fase neonatal reflete o desenvolvimento fisiológico do filhote e contribui para o conhecimento do processo adaptativo em gatos neonatos durante o primeiro mês de vida, sendo útil para a avaliação clínica, diagnóstico e tratamento das doenças neonatais.(AU)

The purpose of the study was to evaluate the effects of age on blood count, liver enzymes alanine aminotransferase (ALT) and gamma-glutamyltransferase (GGT), glycemia and on protein profile in mixed-breed cats during the neonatal phase. Twenty male and female cats were used from 3 to 38 days after birth. Weekly blood samples were obtained and laboratory analyses (blood count, liver enzymes, glycemia and protein profile) were performed on the 3rd, 10th, 17th, 24th, 31st and 38th day of age. The results exhibited significant effect of age on total red blood cell count, hemoglobin concentration, packed cell volume, mean cell volume, mean cell hemoglobin concentration, total leukocyte count, mean segmented neutrophils, eosinophils and basophils. No effect was observed in lymphocyte, monocyte and glucose concentration. The analysis of changes in laboratory parameters which occurred during the neonatal period reflects the physiological development of the kitten and contributes to knowledge of the adaptive process in neonates cats during the first month of life, and is useful for clinical assessment, diagnosis and treatment of neonatal diseases.(AU)
Descritores: Gatos/sangue
Monitoramento
Alanina Transaminase
gama-Glutamiltransferase
-Recém-Nascido
Enzimas
Limites: Animais
Feminino
Gatos
Responsável: BR68.1 - Biblioteca Virginie Buff D'Ápice


  9 / 480 LILACS  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
Texto completo
Id: biblio-1807
Autor: Binoti, Mirella; Ramos, Afonso.
Título: Conservação de alimentos: uma visão mais saudável / Food preservation: a healthier vision
Fonte: HU rev;41(3/4):171-179, dez. 2015.
Idioma: pt.
Resumo: Os consumidores estão cada vez mais conscientes da importância de uma alimentação saudável e livre de contaminação, buscando alimentos que atendam a essa necessidade. O presente trabalho teve como objetivo fazer um levantamento bibliográfico através de artigos atuais encontrados nas bases de dados Scielo, Periódicos Capes, Science Direct, sobre duas metodologias não térmicas de conservação: a alta pressão hidrostática e o campo elétrico pulsado. Ambas possuem a características de eliminar microrganismos e inativar enzimas, ao mesmo tempo em que, provocam mínima ou nenhuma, alteração no valor nutricional dos alimentos.
Descritores: Alimentos
Conservação de Alimentos
-Enzimas
Dieta Saudável
Valor Nutritivo
Tipo de Publ: Relatos de Casos
Responsável: BR378.1 - Biblioteca Central


  10 / 480 LILACS  
              first record previous record
seleciona
para imprimir
Fotocópia
Texto completo SciELO Brasil
Texto completo
Id: lil-601756
Autor: Carrijo, Lanna Clicia; Lima e Borges, Eduardo Euclydes de; Rezende, Sebastião Tavares de; Pontes, Cláudia Aparecida; Silva, Aderlan Gomes da; Lopes, Mariana Rocha.
Título: Avaliação da concentração de proteínas e da atividade de α -galactosidases nos cotilédones e no eixo embrionário de sementes de Dalbergia nigra durante a germinação / Proteins and activity of α -galactosidases determination in cotyledons and embryonic axis of Dalbergia nigra seeds during germination
Fonte: Acta amaz;41(4):465-470, 2011. ilus.
Idioma: pt.
Resumo: Este trabalho teve como objetivos a quantificação de proteínas e da atividade da enzima α-galactosidase, no eixo embrionário e nos cotilédones, de sementes de Dalbergia nigra (jacarandá-da-bahia) durante a germinação. As sementes foram colocadas para embeber em água por sete dias, sendo retiradas amostras para a avaliação bioquímica e cinética da enzima. A atividade da enzima α-galactosidase aumenta com a embebição das sementes nos dois compartimentos, embora não esteja presente no eixo embrionário de sementes secas. A diferença na atividade da enzima entre os cotilédones e o eixo embrionário foi significativa. O pH 5,5 foi o de máxima atividade para as enzimas de ambos os compartimentos. A temperatura que mais estimulou a atividade da enzima nos cotilédones foi 50 ºC e de 50 a 60 ºC no eixo embrionário. A atividade da α-galactosidase foi inibida por β-mercaptoetanol e cobre, em ambos os compartimentos, enquanto a lactose e o cloreto de sódio estimularam a atividade tanto nos cotilédones como no eixo embrionário. Os valores de K M para enzimas do eixo embrionário e dos cotilédones foram de 0,239 e 0,228 mM, respectivamente.

This work aimed to quantify the protein content and the α-galactosidase activity in the embryonic axis and in the cotyledons of Dalbergia nigra seeds during the imbibition period. The seeds were submitted to water imbibition during seven days. Biochemical and kinetic characterization of the enzyme were done from samples taken during the imbibition period. The activity of the α-galactosidase increased in the two compartments with the soaking of the seeds, although, the enzyme activity was not detected in the embryonic axis of dry seeds. The difference in the activity of the enzyme between cotyledons and embryonic axis was significant. The pH of maximum activity was 5.5 for the enzyme of both compartments. In the cotyledons the higher activity of the enzyme was obtained at 50 ºC. In the embryonic axis the activity was higher at temperatures ranging from 50 to 60 ºC. The activity of the α-galactosidase was inhibited by β-mercaptoethanol and CuSO4 in both compartments, while lactose and sodium chloride stimulated the activity in the cotyledons and in the embryonic axis. The values of K M for the enzymes of the embryonic axis and cotyledons were respectively 0.239 and 0.228 mM.
Descritores: Enzimas
Enzima Ramificadora de 1,4-alfa-Glucana
-Bioquímica
Responsável: BR6.1 - BCS - Biblioteca de Ciências da Saúde



página 1 de 48 ir para página                         
   


Refinar a pesquisa
  Base de dados : Formulário avançado   

    Pesquisar no campo  
1  
2
3
 
           



Search engine: iAH v2.6 powered by WWWISIS

BIREME/OPAS/OMS - Centro Latino-Americano e do Caribe de Informação em Ciências da Saúde