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Pesquisa : D08.811.277 [Categoria DeCS]
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Id: biblio-1223238
Autor: Li, Xianjun; Wang, Junhuan; Wu, Wei; Jia, Yang; Fan, Shuanghu; Su Hlaing, Thet; Khokhar, Ibatsam; Yan, Yanchun.
Título: Cometabolic biodegradation of quizalofop-p-ethyl by Methylobacterium populi YC-XJ1 and identification of QPEH1 esterase
Fonte: Electron. j. biotechnol;46:38-49, jul. 2020. ilus, graf.
Idioma: en.
Projeto: National Natural Science Foundation of China; . Basic Research Fund of Chinese Academy of Agricultural Sciences.
Resumo: BACKGROUND: Quizalofop-p-ethyl (QPE), a unitary R configuration aromatic oxyphenoxypropionic acid ester (AOPP) herbicide, was widely used and had led to detrimental environmental effects. For finding the QPEdegrading bacteria and promoting the biodegradation of QPE, a series of studies were carried out. RESULTS: A QPE-degrading bacterial strain YC-XJ1 was isolated from desert soil and identified as Methylobacterium populi, which could degrade QPE with methanol by cometabolism. Ninety-seven percent of QPE (50 mg/L) could be degraded within 72 h under optimum biodegradation condition of 35°C and pH 8.0. The maximum degradation rate of QPE was 1.4 mg/L/h, and the strain YC-XJ1 exhibited some certain salinity tolerance. Two novel metabolites, 2-hydroxy-6-chloroquinoxaline and quinoxaline, were found by high-performance liquid chromatography/mass spectroscopy analysis. The metabolic pathway of QPE was predicted. The catalytic efficiency of strain YC-XJ1 toward different AOPPs herbicides in descending order was as follows: haloxyfop-pmethyl ≈ diclofop-methyl ≈ fluazifop-p-butyl N clodinafop-propargyl N cyhalofop-butyl N quizalofop-p-ethyl N fenoxaprop-p-ethyl N propaquizafop N quizalofop-p-tefuryl. The genome of strain YC-XJ1 was sequenced using a combination of PacBio RS II and Illumina platforms. According to the annotation result, one α/ß hydrolase gene was selected and named qpeh1, for which QPE-degrading function has obtained validation. Based on the phylogenetic analysis and multiple sequence alignment with other QPE-degrading esterases reported previously, the QPEH1 was clustered with esterase family V. CONCLUSION: M. populi YC-XJ1 could degrade QPE with a novel pathway, and the qpeh1 gene was identified as one of QPE-degrading esterase gene.
Descritores: Propionatos/metabolismo
Quinoxalinas/metabolismo
Methylobacterium/metabolismo
-Microbiologia do Solo
Biodegradação Ambiental
Methylobacterium/enzimologia
Methylobacterium/genética
Análise de Sequência de Proteína
Esterases/análise
Esterases/metabolismo
Herbicidas
Hidrolases/análise
Hidrolases/metabolismo
Hidrólise
Responsável: CL1.1 - Biblioteca Central


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Id: biblio-1057282
Autor: Ataides, Fábio Silvestre; Costa, Carolina Rodrigues; Santos, Andressa Santana; Freitas, Vivianny Aparecida Queiroz; Silva, Thaisa Cristina; Zara, Ana Laura Sene Amâncio; Jesuino, Rosália Santos Amorim; Silva, Maria Rosário Rodrigues.
Título: In vitro characterization of virulence factors among species of the Candida parapsilosis complex
Fonte: Rev. Soc. Bras. Med. Trop;53:e20190336, 2020. tab, graf.
Idioma: en.
Projeto: National Counsel of Technological and Scientific Development.
Resumo: Abstract INTRODUCTION: Candida parapsilosis complex species differ from each other with regard to their prevalence and virulence. METHODS: The hydrolytic enzyme activity, biofilm production, and adhesion to epithelial cells were analyzed in 87 C. parapsilosis complex strains. RESULTS: Among the studied isolates, 97.7%, 63.2%, and 82.8% exhibited very strong proteinase, esterase, and hemolysin activity, respectively. All the C. parapsilosis complex isolates produced biofilms and presented an average adherence of 96.0 yeasts/100 epithelial cells. CONCLUSIONS: Our results show that Candida parapsilosis complex isolates showed different levels of enzyme activity, biofilm production, and adhesion to epithelial cells.
Descritores: Fatores de Virulência/análise
Candida parapsilosis/patogenicidade
-Adesão Celular
Técnicas de Tipagem Micológica
Biofilmes/crescimento & desenvolvimento
Candida parapsilosis/isolamento & purificação
Candida parapsilosis/classificação
Candida parapsilosis/enzimologia
Hidrolases/biossíntese
Limites: Humanos
Responsável: BR1.1 - BIREME


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Id: biblio-1147195
Autor: Ahmed, Mohamed Morsi M; Khan, Md. Mohibul Alam; Al-Garni, Saleh M. S; Bora, Roop Singh; Kabli, Saleh A.
Título: Comparative molecular studies of halophilic bacteria from saline water and soil in the Saudi environment / Estudos moleculares comparativos de bactérias halofílicas de água salina e solo no meio ambiente saudita
Fonte: Biosci. j. (Online);36(3):1024-1031, 01-05-2020. tab, ilus.
Idioma: en.
Resumo: Halophilic bacteria are microorganisms that grow optimally in the presence of the very high concentration of sodium chloride. Halophiles are vital sources of various enzymes including hydrolases, which are very stable and catalytically highly efficient at high salt concentration and other extreme conditions such as high temperature, pH and presence of organic solvents. Several hydrolases such as amylases, proteases, and lipases have been obtained from halophilic bacteria and are commonly used for various industrial applications. We initiated a screening to isolate and characterize the halophilic bacteria from the Red Sea, which is one of the saltiest bodies of water in the world. Water and soil samples, collected from the Red Sea coast, Jeddah, Saudi Arabia, were screened for isolation of halophilic bacteria. Ten bacterial isolates were obtained, which were characterized by biochemical tests and 16S rRNA gene sequencing. Hydrolase producing bacteria among the isolates were screened by plate assay on starch and gelatin agar plates for amylase and protease, respectively. Two bacterial isolates i.e. Bacillus glycinifermentans S3 and Enterobacter cloacae W1were found to possess significant amylase and protease activity.

Bactérias halofílicas são microrganismos que crescem de maneira ideal na presença de uma concentração muito alta de cloreto de sódio. Halófilos são fontes vitais de várias enzimas, incluindo hidrolases, que são muito estáveis e cataliticamente altamente eficientes em alta concentração de sal e outras condições extremas, como alta temperatura, pH e presença de solventes orgânicos. Várias hidrolases como amilases, proteases e lipases foram obtidas a partir de bactérias halofílicas e são comumente usadas para várias aplicações industriais. Iniciamos uma triagem para isolar e caracterizar as bactérias halofílicas do Mar Vermelho, que é um dos corpos de água mais salgados do mundo. Amostras de água e solo, coletadas na costa do Mar Vermelho, Jeddah, na Arábia Saudita, foram examinadas quanto ao isolamento de bactérias halofílicas. Foram obtidos dez isolados bacterianos, caracterizados por testes bioquímicos e seqüenciamento do gene 16S rRNA. As bactérias produtoras de hidrolase entre os isolados foram triadas por ensaio em placa em placas de amido e ágar de gelatina para amilase e protease, respectivamente. Verificou-se que dois isolados bacterianos, isto é, Bacillus glycinifermentans S3 e Enterobacter cloacae W1, possuíam significativa atividade de amilase e protease.
Descritores: Peptídeo Hidrolases
Halobacteriales
Salinidade
Amilases
Hidrolases
Responsável: BR396.1 - Biblioteca Central


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Id: biblio-974310
Autor: Souza, Renata Carolini; Cantão, Maurício Egídio; Nogueira, Marco Antonio; Vasconcelos, Ana Tereza Ribeiro; Hungria, Mariangela.
Título: Outstanding impact of soil tillage on the abundance of soil hydrolases revealed by a metagenomic approach
Fonte: Braz. j. microbiol;49(4):723-730, Oct.-Dec. 2018. graf.
Idioma: en.
Projeto: CNPq-Universal; . CNPq; . Embrapa.
Resumo: ABSTRACT The soil represents the main source of novel biocatalysts and biomolecules of industrial relevance. We searched for hydrolases in silico in four shotgun metagenomes (4,079,223 sequences) obtained in a 13-year field trial carried out in southern Brazil, under the no-tillage (NT), or conventional tillage (CT) managements, with crop succession (CS, soybean/wheat), or crop rotation (CR, soybean/maize/wheat/lupine/oat). We identified 42,631 hydrolases belonging to five classes by comparing with the KEGG database, and 44,928 sequences by comparing with the NCBI-NR database. The abundance followed the order: lipases > laccases > cellulases > proteases > amylases > pectinases. Statistically significant differences were attributed to the tillage system, with the NT showing about five times more hydrolases than the CT system. The outstanding differences can be attributed to the management of crop residues, left on the soil surface in the NT, and mechanically broken and incorporated into the soil in the CT. Differences between the CS and the CR were slighter, 10% higher for the CS, but not statistically different. Most of the sequences belonged to fungi (Verticillium, and Colletotrichum for lipases and laccases, and Aspergillus for proteases), and to the archaea Sulfolobus acidocaldarius for amylases. Our results indicate that agricultural soils under conservative managements may represent a hotspot for bioprospection of hydrolases.
Descritores: Solo/química
Proteínas Fúngicas/genética
Archaea/enzimologia
Proteínas Arqueais/genética
Fungos/enzimologia
Hidrolases/genética
-Microbiologia do Solo
Soja/crescimento & desenvolvimento
Triticum/crescimento & desenvolvimento
Brasil
Archaea/isolamento & purificação
Archaea/classificação
Archaea/genética
Zea mays/crescimento & desenvolvimento
Agricultura
Metagenoma
Metagenômica
Fungos/isolamento & purificação
Fungos/classificação
Fungos/genética
Responsável: BR1.1 - BIREME


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Ximenes, Eduardo de Aquino
Id: biblio-855809
Autor: Ximenes, Eduardo de Aquino.
Título: Fungos anaeróbios / Anaerobic fungi
Fonte: Rev. Ciênc. Méd. Biol. (Impr.) = J. med. biol. sci;2(2):269-275, jul.-dez. 2003. ilus.
Idioma: pt.
Resumo: A descoberta de fungos anaeróbios obrigatórios no rume de ovelha por Orpin, em 1975, impulsionou estudos sobre tais microrganismos em laboratórios de diferentes partes do mundo. Estes estudos têm mostrado que os fungos anaeróbios não contêm mitocôndrias; em vez disso, apresentam organelas conhecidas como hidrogenossomas, que estão envolvidas na geração de energia. Estes fungos ainda produzem enzimas que são encontradas sob a forma de um complexo multiprotéico de alta massa molecular, similar ao celulossoma produzido por bactérias anaeróbias, ou individualmente. Diferentes enzimas hidrolíticas, produzidas por variadas espécies destes fungos, principalmente celulolíticas e hemicelulolíticas, têm sido isoladas e caracterizadas. Estas enzimas, em geral, têm atividades específicas similares ou maiores do que outras produzidas por fontes aeróbias correspondentes, tendo, portanto, grande potencial para aplicação industrial
Descritores: Hidrolases
Micoses
Ovinos/microbiologia
Responsável: BR337.1 - Biblioteca


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Id: lil-775109
Autor: Singh, Rashmi; Maurya, Sudarshan; Upadhyay, Ram Sanmukh.
Título: The improvement of competitive saprophytic capabilities of Trichoderma species through the use of chemical mutagens
Fonte: Braz. j. microbiol;47(1):10-17, Jan.-Mar. 2016. graf.
Idioma: en.
Resumo: Abstract The antagonistic potential of Trichoderma strains was assayed by studying the effect of their culture filtrate on the radial growth of Sclerotium rolfsii, the causal agent of chickpea collar rot. Trichoderma harzianum-1432 (42.2%) and Trichoderma atroviride (40.3%) were found to be strong antagonists. To enhance their antagonistic potential, mutagenesis of these two selected strains was performed. Two mutants, Th-m1 and T. atroviride m1, were found to be more effective than their parent strains. The enzymatic activities of the selected parent and mutant strains were assayed, and although both mutants were found to have enhanced enzymatic activities compared to their respective parent strains, Th-m1 possessed the maximum cellulase (5.69 U/mL) and β-1,3-glucanase activity (61.9 U/mL). Th-m1 also showed high competitive saprophytic ability (CSA) among all of the selected parent and mutant strains, and during field experiments, Th-m1 was found to successfully possess enhanced disease control (82.9%).
Descritores: Antibiose/efeitos dos fármacos
Basidiomycota/crescimento & desenvolvimento
Mutagênese
Mutagênicos/metabolismo
Doenças das Plantas/prevenção & controle
Trichoderma/efeitos dos fármacos
Trichoderma/fisiologia
-Cicer/microbiologia
Hidrolases/análise
Mutação
Doenças das Plantas/microbiologia
Trichoderma/enzimologia
Trichoderma/crescimento & desenvolvimento
Responsável: BR1.1 - BIREME


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Id: lil-749729
Autor: Oliveira, Lilian C.G.; Ramos, Patricia Locosque; Marem, Alyne; Kondo, Marcia Y.; Rocha, Rafael C.S.; Bertolini, Thiago; Silveira, Marghuel A.V.; Cruz, João Batista da; Vasconcellos, Suzan Pantaroto de; Juliano, Luiz; Okamoto, Debora N..
Título: Halotolerant bacteria in the São Paulo Zoo composting process and their hydrolases and bioproducts
Fonte: Braz. j. microbiol;46(2):347-354, Apr-Jun/2015. tab, graf.
Idioma: en.
Projeto: Fundação de Amparo a Pesquisa do Estado de São Paulo; . Conselho Nacional de Desenvolvimento Científico e Tecnológico.
Resumo: Halophilic microorganisms are able to grow in the presence of salt and are also excellent source of enzymes and biotechnological products, such as exopolysaccharides (EPSs) and polyhydroxyalkanoates (PHAs). Salt-tolerant bacteria were screened in the Organic Composting Production Unit (OCPU) of São Paulo Zoological Park Foundation, which processes 4 ton/day of organic residues including plant matter from the Atlantic Rain Forest, animal manure and carcasses and mud from water treatment. Among the screened microorganisms, eight halotolerant bacteria grew at NaCl concentrations up to 4 M. These cultures were classified based on phylogenetic characteristics and comparative partial 16S rRNA gene sequence analysis as belonging to the genera Staphylococcus, Bacillus and Brevibacterium. The results of this study describe the ability of these halotolerant bacteria to produce some classes of hydrolases, namely, lipases, proteases, amylases and cellulases, and biopolymers. The strain characterized as of Brevibacterium avium presented cellulase and amylase activities up to 4 M NaCl and also produced EPSs and PHAs. These results indicate the biotechnological potential of certain microorganisms recovered from the composting process, including halotolerant species, which have the ability to produce enzymes and biopolymers, offering new perspectives for environmental and industrial applications.
Descritores: Bacillus/isolamento & purificação
Produtos Biológicos/análise
Brevibacterium/isolamento & purificação
Hidrolases/análise
Microbiologia do Solo
Cloreto de Sódio/metabolismo
Staphylococcus/isolamento & purificação
-Brasil
Bacillus/classificação
Bacillus/genética
Bacillus/metabolismo
Brevibacterium/classificação
Brevibacterium/genética
Brevibacterium/metabolismo
Análise por Conglomerados
DNA Bacteriano/química
DNA Bacteriano/genética
DNA Ribossômico/química
DNA Ribossômico/genética
Dados de Sequência Molecular
Filogenia
/genética
RNA, RIBOSOMAL, ABNORMALITIES, MULTIPLES/genética
Análise de Sequência de DNA
Solo
Staphylococcus/classificação
Staphylococcus/genética
Staphylococcus/metabolismo
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


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Id: lil-748247
Autor: Bezerra, Jadson D.P.; Nascimento, Carlos C.F.; Barbosa, Renan do N.; Silva, Dianny C.V. da; Svedese, Virgínia M.; Silva-Nogueira, Eliane B.; Gomes, Bruno S.; Paiva, Laura M.; Souza-Motta, Cristina M..
Título: Endophytic fungi from medicinal plant Bauhinia forficata: Diversity and biotechnological potential
Fonte: Braz. j. microbiol;46(1):49-57, 05/2015. tab, graf.
Idioma: en.
Resumo: Bauhinia forficata is native to South America and used with relative success in the folk medicine in Brazil. The diversity, antibacterial activity, and extracellular hydrolytic enzymes of endophytic fungi associated with this plant were studied. Plant samples, which included leaves, sepals, stems, and seeds, were used. Ninety-five endophytic fungal were isolated (18 from leaves, 22 from sepals, 46 from stems, and nine from seeds), comprising 28 species. The most frequently isolated species were Acremonium curvulum (9.5%), Aspergillus ochraceus (7.37%), Gibberella fujikuroi (10.53%), Myrothecium verrucaria (10.53%) and Trichoderma piluliferum (7.37%). Diversity and species richness were higher in stem tissues, and Sorensen’s index of similarity between the tissues was low. Eleven fungi showed antibacterial activity. Aspergillus ochraceus, Gibberella baccata, Penicillium commune, and P. glabrum were those with the greatest antibacterial activity against Staphylococcus aureus and/or Streptococcus pyogenes. Thirteen species showed proteolytic activity, particularly Phoma putaminum. Fourteen species were cellulase positive, particularly the Penicillium species and Myrmecridium schulzeri. All isolates tested were xylanase positive and 10 showed lipolytic activity, especially Penicillium glabrum. It is clear that the endophytic fungi from B. forficata have potential for the production of bioactive compounds and may be a source of new therapeutic agents for the effective treatment of diseases in humans, other animals, and plants. To our knowledge, this is the first study of endophytic fungi from different tissues of B. forficata and their biotechnological potential.
Descritores: Anti-Infecciosos/metabolismo
Biodiversidade
Bauhinia/microbiologia
Endófitos/classificação
Fungos/metabolismo
Hidrolases/metabolismo
Plantas Medicinais/microbiologia
-Brasil
Bactérias/efeitos dos fármacos
Produtos Biológicos/metabolismo
Biotecnologia/métodos
Endófitos/isolamento & purificação
Endófitos/metabolismo
Fungos/classificação
Fungos/isolamento & purificação
Testes de Sensibilidade Microbiana
América do Sul
Tecnologia Farmacêutica/métodos
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


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Id: lil-741304
Autor: Rehman, Shazia; Aslam, Hina; Ahmad, Aqeel; Khan, Shakeel Ahmed; Sohail, Muhammad.
Título: Production of plant cell wall degrading enzymes by monoculture and co-culture of Aspergillus niger and Aspergillus terreus under SSF of banana peels
Fonte: Braz. j. microbiol;45(4):1485-1492, Oct.-Dec. 2014. graf, tab.
Idioma: en.
Resumo: Filamentous fungi are considered to be the most important group of microorganisms for the production of plant cell wall degrading enzymes (CWDE), in solid state fermentations. In this study, two fungal strains Aspergillus niger MS23 and Aspergillus terreus MS105 were screened for plant CWDE such as amylase, pectinase, xylanase and cellulases (β-glucosidase, endoglucanase and filterpaperase) using a novel substrate, Banana Peels (BP) for SSF process. This is the first study, to the best of our knowledge, to use BP as SSF substrate for plant CWDE production by co-culture of fungal strains. The titers of pectinase were significantly improved in co-culture compared to mono-culture. Furthermore, the enzyme preparations obtained from monoculture and co-culture were used to study the hydrolysis of BP along with some crude and purified substrates. It was observed that the enzymatic hydrolysis of different crude and purified substrates accomplished after 26 h of incubation, where pectin was maximally hydrolyzed by the enzyme preparations of mono and co-culture. Along with purified substrates, crude materials were also proved to be efficiently degraded by the cocktail of the CWDE. These results demonstrated that banana peels may be a potential substrate in solid-state fermentation for the production of plant cell wall degrading enzymes to be used for improving various biotechnological and industrial processes.
Descritores: Aspergillus/enzimologia
Aspergillus/crescimento & desenvolvimento
Hidrolases/metabolismo
Musa/metabolismo
Musa/microbiologia
-Aspergillus/metabolismo
Técnicas de Cocultura
Fermentação
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


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Id: lil-732581
Autor: GORDAN, Valeria Veiga; McEDWARD, Deborah Landry; OTTENGA, Marc Edward; GARVAN, Cynthia Wilson; HARRIS, Pearl Ann.
Título: Alkali production in the mouth and its relationship with certain patient's characteristics
Fonte: J. appl. oral sci;22(6):560-568, Nov-Dec/2014. tab, graf.
Idioma: en.
Projeto: NIH-NIDCR.
Resumo: Objectives To assess the relationships among alkali production, diet, oral health behaviors, and oral hygiene. Methods Data from 52 subjects including demographics, diet, and oral hygiene scores were analyzed against the level of arginine and urea enzymes in plaque and saliva samples. An oral habit survey was completed that included: use of tobacco (TB), alcohol (AH), sugary drinks (SD), and diet. Alkali production through arginine deiminase (ADS) and urease activities were measured in smooth-surface supragingival dental plaque and un stimulated saliva samples from all subjects. ADS and urease activities were measured by quantification of the ammonia generated from the incubation of plaque or saliva samples. Spearman correlations were used to compute all associations. Results Participants in the lowest SES (Socio-economic status) group had the habit of consuming sugary drinks the most and had the highest rate of tobacco use. Males consumed significantly more alcohol than females. No significant relationship was found between age or gender and alkali production. Higher rates of sugary drink consumption and tobacco use were significantly related to lower alkali production. Conclusion The study showed a relationship between alkali production and oral hygiene, diet, and certain oral health behaviors. Poor oral hygiene was significantly associated with age, lower SES, tobacco use, and alcohol, and sugary drinks consumption. Clinical relevance Certain oral health behaviors have an impact on oral hygiene and on alkali production; it is important to address these factors with patients as a strategy for caries control. .
Descritores: Álcalis/análise
Comportamento Alimentar
Boca/química
Higiene Bucal
-Fatores Etários
Consumo de Bebidas Alcoólicas/efeitos adversos
Álcalis/metabolismo
Carboidratos/efeitos adversos
Cárie Dentária/etiologia
Cárie Dentária/prevenção & controle
Hidrolases/análise
Hidrolases/metabolismo
Fatores de Risco
Fatores Socioeconômicos
Estatísticas não Paramétricas
Saliva/química
Fumar/efeitos adversos
Urease/análise
Urease/metabolismo
Limites: Adulto
Feminino
Humanos
Masculino
Adulto Jovem
Tipo de Publ: Research Support, N.I.H., Extramural
Responsável: BR1.1 - BIREME



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