Base de dados : LILACS
Pesquisa : D08.811.277 [Categoria DeCS]
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Id: biblio-974310
Autor: Souza, Renata Carolini; Cantão, Maurício Egídio; Nogueira, Marco Antonio; Vasconcelos, Ana Tereza Ribeiro; Hungria, Mariangela.
Título: Outstanding impact of soil tillage on the abundance of soil hydrolases revealed by a metagenomic approach
Fonte: Braz. j. microbiol;49(4):723-730, Oct.-Dec. 2018. graf.
Idioma: en.
Projeto: CNPq-Universal; . CNPq; . Embrapa.
Resumo: ABSTRACT The soil represents the main source of novel biocatalysts and biomolecules of industrial relevance. We searched for hydrolases in silico in four shotgun metagenomes (4,079,223 sequences) obtained in a 13-year field trial carried out in southern Brazil, under the no-tillage (NT), or conventional tillage (CT) managements, with crop succession (CS, soybean/wheat), or crop rotation (CR, soybean/maize/wheat/lupine/oat). We identified 42,631 hydrolases belonging to five classes by comparing with the KEGG database, and 44,928 sequences by comparing with the NCBI-NR database. The abundance followed the order: lipases > laccases > cellulases > proteases > amylases > pectinases. Statistically significant differences were attributed to the tillage system, with the NT showing about five times more hydrolases than the CT system. The outstanding differences can be attributed to the management of crop residues, left on the soil surface in the NT, and mechanically broken and incorporated into the soil in the CT. Differences between the CS and the CR were slighter, 10% higher for the CS, but not statistically different. Most of the sequences belonged to fungi (Verticillium, and Colletotrichum for lipases and laccases, and Aspergillus for proteases), and to the archaea Sulfolobus acidocaldarius for amylases. Our results indicate that agricultural soils under conservative managements may represent a hotspot for bioprospection of hydrolases.
Descritores: Solo/química
Proteínas Fúngicas/genética
Archaea/enzimologia
Proteínas Arqueais/genética
Fungos/enzimologia
Hidrolases/genética
-Microbiologia do Solo
Soja/crescimento & desenvolvimento
Triticum/crescimento & desenvolvimento
Brasil
Archaea/isolamento & purificação
Archaea/classificação
Archaea/genética
Zea mays/crescimento & desenvolvimento
Agricultura
Metagenoma
Metagenômica
Fungos/isolamento & purificação
Fungos/classificação
Fungos/genética
Responsável: BR1.1 - BIREME


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Ximenes, Eduardo de Aquino
Id: biblio-855809
Autor: Ximenes, Eduardo de Aquino.
Título: Fungos anaeróbios / Anaerobic fungi
Fonte: Rev. Ciênc. Méd. Biol. (Impr.) = J. med. biol. sci;2(2):269-275, jul.-dez. 2003. ilus.
Idioma: pt.
Resumo: A descoberta de fungos anaeróbios obrigatórios no rume de ovelha por Orpin, em 1975, impulsionou estudos sobre tais microrganismos em laboratórios de diferentes partes do mundo. Estes estudos têm mostrado que os fungos anaeróbios não contêm mitocôndrias; em vez disso, apresentam organelas conhecidas como hidrogenossomas, que estão envolvidas na geração de energia. Estes fungos ainda produzem enzimas que são encontradas sob a forma de um complexo multiprotéico de alta massa molecular, similar ao celulossoma produzido por bactérias anaeróbias, ou individualmente. Diferentes enzimas hidrolíticas, produzidas por variadas espécies destes fungos, principalmente celulolíticas e hemicelulolíticas, têm sido isoladas e caracterizadas. Estas enzimas, em geral, têm atividades específicas similares ou maiores do que outras produzidas por fontes aeróbias correspondentes, tendo, portanto, grande potencial para aplicação industrial
Descritores: Hidrolases
Micoses
Ovinos/microbiologia
Responsável: BR337.1 - Biblioteca


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Id: lil-775109
Autor: Singh, Rashmi; Maurya, Sudarshan; Upadhyay, Ram Sanmukh.
Título: The improvement of competitive saprophytic capabilities of Trichoderma species through the use of chemical mutagens
Fonte: Braz. j. microbiol;47(1):10-17, Jan.-Mar. 2016. graf.
Idioma: en.
Resumo: Abstract The antagonistic potential of Trichoderma strains was assayed by studying the effect of their culture filtrate on the radial growth of Sclerotium rolfsii, the causal agent of chickpea collar rot. Trichoderma harzianum-1432 (42.2%) and Trichoderma atroviride (40.3%) were found to be strong antagonists. To enhance their antagonistic potential, mutagenesis of these two selected strains was performed. Two mutants, Th-m1 and T. atroviride m1, were found to be more effective than their parent strains. The enzymatic activities of the selected parent and mutant strains were assayed, and although both mutants were found to have enhanced enzymatic activities compared to their respective parent strains, Th-m1 possessed the maximum cellulase (5.69 U/mL) and β-1,3-glucanase activity (61.9 U/mL). Th-m1 also showed high competitive saprophytic ability (CSA) among all of the selected parent and mutant strains, and during field experiments, Th-m1 was found to successfully possess enhanced disease control (82.9%).
Descritores: Antibiose/efeitos dos fármacos
Basidiomycota/crescimento & desenvolvimento
Mutagênese
Mutagênicos/metabolismo
Doenças das Plantas/prevenção & controle
Trichoderma/efeitos dos fármacos
Trichoderma/fisiologia
-Cicer/microbiologia
Hidrolases/análise
Mutação
Doenças das Plantas/microbiologia
Trichoderma/enzimologia
Trichoderma/crescimento & desenvolvimento
Responsável: BR1.1 - BIREME


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Id: lil-749729
Autor: Oliveira, Lilian C.G.; Ramos, Patricia Locosque; Marem, Alyne; Kondo, Marcia Y.; Rocha, Rafael C.S.; Bertolini, Thiago; Silveira, Marghuel A.V.; Cruz, João Batista da; Vasconcellos, Suzan Pantaroto de; Juliano, Luiz; Okamoto, Debora N..
Título: Halotolerant bacteria in the São Paulo Zoo composting process and their hydrolases and bioproducts
Fonte: Braz. j. microbiol;46(2):347-354, Apr-Jun/2015. tab, graf.
Idioma: en.
Projeto: Fundação de Amparo a Pesquisa do Estado de São Paulo; . Conselho Nacional de Desenvolvimento Científico e Tecnológico.
Resumo: Halophilic microorganisms are able to grow in the presence of salt and are also excellent source of enzymes and biotechnological products, such as exopolysaccharides (EPSs) and polyhydroxyalkanoates (PHAs). Salt-tolerant bacteria were screened in the Organic Composting Production Unit (OCPU) of São Paulo Zoological Park Foundation, which processes 4 ton/day of organic residues including plant matter from the Atlantic Rain Forest, animal manure and carcasses and mud from water treatment. Among the screened microorganisms, eight halotolerant bacteria grew at NaCl concentrations up to 4 M. These cultures were classified based on phylogenetic characteristics and comparative partial 16S rRNA gene sequence analysis as belonging to the genera Staphylococcus, Bacillus and Brevibacterium. The results of this study describe the ability of these halotolerant bacteria to produce some classes of hydrolases, namely, lipases, proteases, amylases and cellulases, and biopolymers. The strain characterized as of Brevibacterium avium presented cellulase and amylase activities up to 4 M NaCl and also produced EPSs and PHAs. These results indicate the biotechnological potential of certain microorganisms recovered from the composting process, including halotolerant species, which have the ability to produce enzymes and biopolymers, offering new perspectives for environmental and industrial applications.
Descritores: Bacillus/isolamento & purificação
Produtos Biológicos/análise
Brevibacterium/isolamento & purificação
Hidrolases/análise
Microbiologia do Solo
Cloreto de Sódio/metabolismo
Staphylococcus/isolamento & purificação
-Brasil
Bacillus/classificação
Bacillus/genética
Bacillus/metabolismo
Brevibacterium/classificação
Brevibacterium/genética
Brevibacterium/metabolismo
Análise por Conglomerados
DNA Bacteriano/química
DNA Bacteriano/genética
DNA Ribossômico/química
DNA Ribossômico/genética
Dados de Sequência Molecular
Filogenia
/genética
RNA, RIBOSOMAL, ABNORMALITIES, MULTIPLES/genética
Análise de Sequência de DNA
Solo
Staphylococcus/classificação
Staphylococcus/genética
Staphylococcus/metabolismo
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


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Id: lil-748247
Autor: Bezerra, Jadson D.P.; Nascimento, Carlos C.F.; Barbosa, Renan do N.; Silva, Dianny C.V. da; Svedese, Virgínia M.; Silva-Nogueira, Eliane B.; Gomes, Bruno S.; Paiva, Laura M.; Souza-Motta, Cristina M..
Título: Endophytic fungi from medicinal plant Bauhinia forficata: Diversity and biotechnological potential
Fonte: Braz. j. microbiol;46(1):49-57, 05/2015. tab, graf.
Idioma: en.
Resumo: Bauhinia forficata is native to South America and used with relative success in the folk medicine in Brazil. The diversity, antibacterial activity, and extracellular hydrolytic enzymes of endophytic fungi associated with this plant were studied. Plant samples, which included leaves, sepals, stems, and seeds, were used. Ninety-five endophytic fungal were isolated (18 from leaves, 22 from sepals, 46 from stems, and nine from seeds), comprising 28 species. The most frequently isolated species were Acremonium curvulum (9.5%), Aspergillus ochraceus (7.37%), Gibberella fujikuroi (10.53%), Myrothecium verrucaria (10.53%) and Trichoderma piluliferum (7.37%). Diversity and species richness were higher in stem tissues, and Sorensen’s index of similarity between the tissues was low. Eleven fungi showed antibacterial activity. Aspergillus ochraceus, Gibberella baccata, Penicillium commune, and P. glabrum were those with the greatest antibacterial activity against Staphylococcus aureus and/or Streptococcus pyogenes. Thirteen species showed proteolytic activity, particularly Phoma putaminum. Fourteen species were cellulase positive, particularly the Penicillium species and Myrmecridium schulzeri. All isolates tested were xylanase positive and 10 showed lipolytic activity, especially Penicillium glabrum. It is clear that the endophytic fungi from B. forficata have potential for the production of bioactive compounds and may be a source of new therapeutic agents for the effective treatment of diseases in humans, other animals, and plants. To our knowledge, this is the first study of endophytic fungi from different tissues of B. forficata and their biotechnological potential.
Descritores: Anti-Infecciosos/metabolismo
Biodiversidade
Bauhinia/microbiologia
Endófitos/classificação
Fungos/metabolismo
Hidrolases/metabolismo
Plantas Medicinais/microbiologia
-Brasil
Bactérias/efeitos dos fármacos
Produtos Biológicos/metabolismo
Biotecnologia/métodos
Endófitos/isolamento & purificação
Endófitos/metabolismo
Fungos/classificação
Fungos/isolamento & purificação
Testes de Sensibilidade Microbiana
América do Sul
Tecnologia Farmacêutica/métodos
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


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Id: lil-741304
Autor: Rehman, Shazia; Aslam, Hina; Ahmad, Aqeel; Khan, Shakeel Ahmed; Sohail, Muhammad.
Título: Production of plant cell wall degrading enzymes by monoculture and co-culture of Aspergillus niger and Aspergillus terreus under SSF of banana peels
Fonte: Braz. j. microbiol;45(4):1485-1492, Oct.-Dec. 2014. graf, tab.
Idioma: en.
Resumo: Filamentous fungi are considered to be the most important group of microorganisms for the production of plant cell wall degrading enzymes (CWDE), in solid state fermentations. In this study, two fungal strains Aspergillus niger MS23 and Aspergillus terreus MS105 were screened for plant CWDE such as amylase, pectinase, xylanase and cellulases (β-glucosidase, endoglucanase and filterpaperase) using a novel substrate, Banana Peels (BP) for SSF process. This is the first study, to the best of our knowledge, to use BP as SSF substrate for plant CWDE production by co-culture of fungal strains. The titers of pectinase were significantly improved in co-culture compared to mono-culture. Furthermore, the enzyme preparations obtained from monoculture and co-culture were used to study the hydrolysis of BP along with some crude and purified substrates. It was observed that the enzymatic hydrolysis of different crude and purified substrates accomplished after 26 h of incubation, where pectin was maximally hydrolyzed by the enzyme preparations of mono and co-culture. Along with purified substrates, crude materials were also proved to be efficiently degraded by the cocktail of the CWDE. These results demonstrated that banana peels may be a potential substrate in solid-state fermentation for the production of plant cell wall degrading enzymes to be used for improving various biotechnological and industrial processes.
Descritores: Aspergillus/enzimologia
Aspergillus/crescimento & desenvolvimento
Hidrolases/metabolismo
Musa/metabolismo
Musa/microbiologia
-Aspergillus/metabolismo
Técnicas de Cocultura
Fermentação
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


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Id: lil-732581
Autor: GORDAN, Valeria Veiga; McEDWARD, Deborah Landry; OTTENGA, Marc Edward; GARVAN, Cynthia Wilson; HARRIS, Pearl Ann.
Título: Alkali production in the mouth and its relationship with certain patient's characteristics
Fonte: J. appl. oral sci;22(6):560-568, Nov-Dec/2014. tab, graf.
Idioma: en.
Projeto: NIH-NIDCR.
Resumo: Objectives To assess the relationships among alkali production, diet, oral health behaviors, and oral hygiene. Methods Data from 52 subjects including demographics, diet, and oral hygiene scores were analyzed against the level of arginine and urea enzymes in plaque and saliva samples. An oral habit survey was completed that included: use of tobacco (TB), alcohol (AH), sugary drinks (SD), and diet. Alkali production through arginine deiminase (ADS) and urease activities were measured in smooth-surface supragingival dental plaque and un stimulated saliva samples from all subjects. ADS and urease activities were measured by quantification of the ammonia generated from the incubation of plaque or saliva samples. Spearman correlations were used to compute all associations. Results Participants in the lowest SES (Socio-economic status) group had the habit of consuming sugary drinks the most and had the highest rate of tobacco use. Males consumed significantly more alcohol than females. No significant relationship was found between age or gender and alkali production. Higher rates of sugary drink consumption and tobacco use were significantly related to lower alkali production. Conclusion The study showed a relationship between alkali production and oral hygiene, diet, and certain oral health behaviors. Poor oral hygiene was significantly associated with age, lower SES, tobacco use, and alcohol, and sugary drinks consumption. Clinical relevance Certain oral health behaviors have an impact on oral hygiene and on alkali production; it is important to address these factors with patients as a strategy for caries control. .
Descritores: Álcalis/análise
Comportamento Alimentar
Boca/química
Higiene Bucal
-Fatores Etários
Consumo de Bebidas Alcoólicas/efeitos adversos
Álcalis/metabolismo
Carboidratos/efeitos adversos
Cárie Dentária/etiologia
Cárie Dentária/prevenção & controle
Hidrolases/análise
Hidrolases/metabolismo
Fatores de Risco
Fatores Socioeconômicos
Estatísticas não Paramétricas
Saliva/química
Fumar/efeitos adversos
Urease/análise
Urease/metabolismo
Limites: Adulto
Feminino
Humanos
Masculino
Adulto Jovem
Tipo de Publ: Research Support, N.I.H., Extramural
Responsável: BR1.1 - BIREME


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Id: lil-715579
Autor: Chicatto, JA; Costa, A; Nunes, H; Helm, CV; Tavares, LBB.
Título: Evaluation of hollocelulase production by Lentinula edodes (Berk.) Pegler during the submerged fermentation growth using RSM / Avaliação da produção de enzimas holocelulolíticas por Lentinula edodes (Berk.) Pegler durante o crescimento em fermentação submersa usando MSR
Fonte: Braz. j. biol;74(1):243-250, 2/2014. tab, graf.
Idioma: en.
Resumo: The cellulase proteins have a great importance in the enzymatic hydrolysis of woody biomass. Despite of costs being a major concern, it has been a stimulus to study basidiomycetes biochemical properties which degrade lignocellulosic material and have prompted the processes' study for obtaining cellulolytic enzymes in fungi. The objective of this research was to evaluate the effects of the initial nitrogen content on (ammonium sulfate) and on sugar cane bagasse, which hereby, acts as an inducer of hydrolytic enzymes to produce cellulases and xylanases, using three Lentinula edodes (Berk.) Pegler strains as a transformation agent. A factorial design with 22 replications in the central point was conducted, varying concentrations of ammonium sulfate and sugar cane bagasse. The submerged cultures carried out in synthetic culture medium and incubated at 25°C for 7 days on an orbital shaker at 150 rpm. The total protein and cellulase activity as endoglucanase, exoglucanase and β-glucosidase and the xylanase was also determined. The results showed that the production of hydrolytic enzymes was stimulated by the presence of high concentrations of sugar cane bagasse (30g/L), characterizing it as an inducer due to the demonstrated proportional relationship. Thus, ammonium sulfate acted as a reducing agent in the synthesis of enzymes, being the low concentrations (0.1g/L) indicated for the enzyme production system under study. Among the studied strains, the EF52 showed higher activity for xylanase, endoglucanases, β-glucosidase and also protein.

As celulases são proteínas de grande importância na hidrólise enzimática de biomassa florestal. No entanto, seu custo elevado tem estimulado o estudo de processos de obtenção de enzimas celulolíticas por fungos filamentosos, tais como os basidiomicetos que apresentam propriedades bioquímicas para degradação de material lignocelulósico. O objetivo deste trabalho foi avaliar os efeitos do teor inicial de nitrogênio (sulfato de amônia) e de um indutor de enzimas hidrolíticas (bagaço de cana de açúcar) na produção de xilanases e celulases utilizando três isolados de Lentinula edodes (Berk.) Pegler como agente de transformação. Foi realizado um planejamento fatorial 22 com repetição no ponto central, variando as concentrações de sulfato de amônia e bagaço de cana de açúcar. O cultivo submerso realizado em meio de cultivo sintético e incubado a 25°C por 7 dias em agitador orbital a 150 rpm. Foram determinados o teor de proteínas totais e a atividade de celulase como: endoglucanase, exoglucanase e β-glucosidase e ainda xilanase. Os resultados demonstraram que a produção das enzimas hidrolíticas foi estimulada pela presença de alta concentração de bagaço de cana (30g/L), caracterizando-o como agente indutor devido à relação de proporcionalidade demonstrada. Por sua vez, o sulfato de amônio atuou como redutor da síntese de enzimas, sendo as baixas concentrações (0,1g/L) indicadas para o sistema de produção das enzimas em estudo. Quanto às linhagens, a EF52 mostrou maior atividade para xilanase, endoglucanases, β-glucosidase e proteínas.
Descritores: Sulfato de Amônio/farmacologia
Celulose/farmacologia
Hidrolases/biossíntese
Saccharum/química
Cogumelos Shiitake/enzimologia
-Fermentação
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


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Id: lil-711711
Autor: REYES, Evelyn; MARTIN, Javier; MONCADA, Gustavo; NEIRA, Miguel; PALMA, Patricia; GORDAN, Valeria; OYARZO, Juan Fernando; YEVENES, Ismael.
Título: Caries-free subjects have high levels of urease and arginine deiminase activity
Fonte: J. appl. oral sci;22(3):235-240, May-Jun/2014. tab.
Idioma: en.
Projeto: FONIS.
Resumo: Objectives: This study investigated the relationship between urease and arginine deiminase system (ADS) activities and dental caries through a cross-sectional study. Material and Methods: Urease and ADS activities were measured in saliva and plaque samples from 10 caries-free subjects and 13 caries-active. Urease activity was obtained from the ammonia produced by incubation of plaque and saliva samples in urea. ADS activity was obtained from the ammonia generated by the arginine-HCl and Tris-maleate buffer. Specific activity was defined as micromoles of ammonia per minute per milligram of protein. Shapiro-Wilk statistical test was used to analyze the distribution of the data, and Mann-Whitney test was used to determine the significance of the data. Results: The specific urease activity in saliva and plaque was significantly higher in individuals with low DMFT scores. ADS activity in saliva (6.050 vs 1.350, p=0.0154) and plaque (8.830 vs 1.210, p=0.025) was also higher in individuals with low DMFT scores. Conclusions: Caries-free subjects had a higher ammonia generation activity by urease and arginine deiminase system for both saliva and plaque samples than low caries-active subjects. High levels of alkali production in oral environment were related to caries-free subjects. .
Descritores: Cárie Dentária/enzimologia
Placa Dentária/enzimologia
Hidrolases/análise
Saliva/enzimologia
Urease/análise
-Álcalis/metabolismo
Amônia/análise
Estudos de Casos e Controles
Estudos Transversais
Índice CPO
Placa Dentária/química
Valores de Referência
Fatores de Risco
Estatísticas não Paramétricas
Saliva/química
Limites: Feminino
Humanos
Masculino
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


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Id: lil-665847
Autor: Kumar, Sumit; Karan, Ram; Kapoor, Sanjay; Singh, S P; Khare, S K.
Título: Screening and isolation of halophilic bacteria producing industrially important enzymes
Fonte: Braz. j. microbiol;43(4):1595-1603, Oct.-Dec. 2012. graf, tab.
Idioma: en.
Resumo: Halophiles are excellent sources of enzymes that are not only salt stable but also can withstand and carry out reactions efficiently under extreme conditions. The aim of the study was to isolate and study the diversity among halophilic bacteria producing enzymes of industrial value. Screening of halophiles from various saline habitats of India led to isolation of 108 halophilic bacteria producing industrially important hydrolases (amylases, lipases and proteases). Characterization of 21 potential isolates by morphological, biochemical and 16S rRNA gene analysis found them related to Marinobacter, Virgibacillus, Halobacillus, Geomicrobium, Chromohalobacter, Oceanobacillus, Bacillus, Halomonas and Staphylococcus genera. They belonged to moderately halophilic group of bacteria exhibiting salt requirement in the range of 3-20%. There is significant diversity among halophiles from saline habitats of India. Preliminary characterization of crude hydrolases established them to be active and stable under more than one extreme condition of high salt, pH, temperature and presence of organic solvents. It is concluded that these halophilic isolates are not only diverse in phylogeny but also in their enzyme characteristics. Their enzymes may be potentially useful for catalysis under harsh operational conditions encountered in industrial processes. The solvent stability among halophilic enzymes seems a generic novel feature making them potentially useful in non-aqueous enzymology.
Descritores: Ativadores de Enzimas/análise
Biodiversidade
Halobacteriales/isolamento & purificação
Hidrolases/análise
Hidrolases/isolamento & purificação
Solventes/análise
-Catálise
Microbiologia Ambiental
Métodos
Tipo de Publ: Estudo Comparativo
Responsável: BR32.1 - Serviço de Biblioteca e Informação Biomédica



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