Base de dados : LILACS
Pesquisa : D08.811.277.040 [Categoria DeCS]
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Id: lil-609919
Autor: Araya, Juan Carlos; Lépez, Macarena; González Álvarez, Raúl; González Luy, Jessica; Depix, María Soledad.
Título: Inmunodetección de FHIT y E-cadherina en fibroadenomas y cáncer ductal y lobulillar de mama / Inmunodetection of FHIT and E-cadherin in fibroadenomas and ductal and lobular human breast cancer
Fonte: Rev. chil. tecnol. méd;31(1):1607-1612, jul. 2011. ilus.
Idioma: es.
Projeto: UST.
Resumo: En Chile, el cáncer es la segunda causa de muerte después de las enfermedades cardiovasculares. Los principales cánceres asociados a muerte en mujeres fueron mama, estómago, vesícula biliar, broncopulmonar y cérvico uterino. Alteraciones de las E-cadherinas han sido relacionadas con varios tipos de cáncer, ya que uno de los principales eventos involucrados con su disfunción es el gatillar la invasión y metástasis del tumor. La inactivación del gen CDH1 ha sido demostrada en el cáncer gástrico difuso y el cáncer de mama lobulillar. Asimismo, la inactivación del gen FHIT parece estar asociado con la progresión a neoplasias más agresivas. Se realizaron determinaciones inmunohistoquímicas (IHQ) en fibroadenomas mamarios y cánceres previamente diagnosticados por RE, RPg y Her2, mostrando positividad en todos los casos. La detección (IHQ) de la expresión de FHIT y E-cadherina en tejidos con patologías benignas y malignizados, puede aportar una importante información diagnóstica y pronóstica en el cáncer de mama.

In Chile, cancer is the second leading cause of death after cardiovascular diseases. The major death-related cancers in women were breast, stomach, gallbladder, lung and cervical cancer. Alterations of E-cadherin have been linked to various cancers, as one of the main events involved in its dysfunction is the trigger of tumor invasion and metastasis. CDH1 gene inactivation has been demonstrated in diffuse gastric cancer and lobular breast cancer. Furthermore, inactivation of the FHIT gene to be associated with progression to more aggressive tumors. Immunohistochemistry (IHC) determinations were performed in fibroadenomas and breast cancers previously diagnosed by ER, PgR and Her2, showing positivity in all cases. Immunohistochemical detection of FHIT and E-cadherin expression in tissues with benign disease and malignant, may provide an important diagnostic and prognostic information in breast cancer.
Descritores: Hidrolases Anidrido Ácido/metabolismo
Caderinas/metabolismo
Neoplasias da Mama/diagnóstico
Neoplasias da Mama/metabolismo
Proteínas de Neoplasias/metabolismo
-Carcinoma Ductal de Mama/diagnóstico
Carcinoma Ductal de Mama/metabolismo
Carcinoma Lobular/diagnóstico
Carcinoma Lobular/metabolismo
Fibroadenoma/diagnóstico
Fibroadenoma/metabolismo
Imuno-Histoquímica
Limites: Humanos
Feminino
Responsável: CL1.1 - Biblioteca Central


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Texto completo SciELO Chile
Texto completo
Id: lil-484920
Autor: Roa S., Juan Carlos; García M., Patricia; Melo A., Angélica; Tapia E., Oscar; Villaseca H., Miguel; Araya O., Juan Carlos; Guzmán G., Pablo.
Título: Estudio de patrones de metilación génica en tumores del tubo digestivo / Gene methylation patterns in digestive tumors
Fonte: Rev. méd. Chile;136(4):451-458, abr. 2008. ilus, tab, graf.
Idioma: es.
Projeto: Universidad de La Frontera.
Resumo: Background: The loss of tumor suppresor gene function damages the defensive mechanisms that protect the indemnity of genetic material. Promoter gene methylation is one of the inactivation mechanisms of suppressor genes. Aim: To study the methylation pattern of a group of genes in biopsy samples of gastrointestinal tumors. Material and methods: Forty eight gastric, 25 gallbladder, 24 colon and 6 pancreas cancer biopsy samples were randomly selected. The methylation pattern of CDH1, FHIT, CDKN2A, APC and MLH1 genes, was studied using a specific polymerase chain reaction test for methylation. Demographic, morphological and follow up variables of patients bearing the tumors were also analyzed. Results: The general methylation frequency of CDH1, FHIT, CDKN2A, APC and MLH1 genes was 64.1, 56, 39.8, 18.1 and 34 percent respectively. In gastric cancer samples there was a correlation between APC gene methylation and well differentiated tumors; between CDH1 methylation and Lauren diffuse type and the presence of three or more metastasic lymph nodes; between FHIT, CDKN2A and CDH1 gene methylation and male gender. In ¡ess differentiated gallbladder tumors, the frequency of CDH1 methylation was higher. There was a tendency towards a lower survival in colon and gastric cancer when MLH1 (p =0.07) y CDKN2A (p= 0.06) were methylated, respectively. Conclusions: An abnormal methylation pattern was associated with morphological features in gastric and gallbladder cancer and with a tendency towards a lower survival in colon and gastric cancer.
Descritores: Carcinoma/genética
Metilação de DNA/genética
Neoplasias da Vesícula Biliar/genética
Neoplasias Gastrointestinais/genética
Neoplasias Pancreáticas/genética
-Hidrolases Anidrido Ácido/genética
Hidrolases Anidrido Ácido/metabolismo
Caderinas/genética
Carcinoma/metabolismo
Neoplasias da Vesícula Biliar/metabolismo
Neoplasias Gastrointestinais/metabolismo
GENES, PABNORMALITIES, MULTIPLE
Estimativa de Kaplan-Meier
Técnicas de Amplificação de Ácido Nucleico
Proteínas de Neoplasias/genética
Proteínas de Neoplasias/metabolismo
Reação em Cadeia da Polimerase
Neoplasias Pancreáticas/metabolismo
Limites: Idoso
Feminino
Humanos
Masculino
Pessoa de Meia-Idade
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


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Texto completo SciELO Chile
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Id: lil-443219
Autor: Roa, J. C; Anabalón, L; Tapia, O; Martínez, J; Araya, J. C; Villaseca, M; Guzmán, P; Roa, I.
Título: Patrón de metilación génico en el cáncer de mama / Promoter methylation profile in breast cancer
Fonte: Rev. méd. Chile;132(9):1069-1077, sept. 2004. tab.
Idioma: es.
Resumo: BACKGROUND: Genomic DNA methylation, mutations and allelic deletions explain the inactivation of genes involved in cell proliferation and cell cycle control mechanisms. AIM: To analyze the methylation pattern of important genes related to different carcinogenic mechanisms in patients with breast cancer and the relationship with its biological behavior. MATERIAL AND METHODS: Seventy fresh-frozen breast cancer samples were selected. The methylation specific PCR (MSP) test was used to analyze promoter methylation status for genes CDKN2A (p16), hMLH1, APC, CDH1 (Cadherin E) and FHIT. RESULTS: We found methylation in at least one of the genes studied in 88% of cases and in 3 or more genes in 40.5% of cases. The frequencies of promoter hypermethylation of CDKN2A, hMLH1, APC, CDH1 and FHT were 41.4%, 11.4%, 52.9%, 70% and 42.9%, respectively. We found a relationship between CDKN2A methylatlon and better survival (p=0.002). CDH1 methylation and poor histological differentiation (p=0.007), hMLH1 methylation and non-Mapuche ethnicity (p=-0.03), APC methylation and larger tumor size (p<0.05), FHIT methylatton and lack of estrogen rectptor IHC expression (p<0.05). CONCLUSIONS: The high frequency of promoter methylation in patients with breast cancer confirms its role in breast carcinogenesis. The finding of alterations in the methylation pattern of genes studied and its association with prognostic factors is a helpful tool in the search of new criteria for clinical and therapeutic decision making.
Descritores: Carcinoma Ductal de Mama/genética
Metilação de DNA
Genes Supressores de Tumor
Neoplasias da Mama/genética
Regiões Promotoras Genéticas
-Amplificação de Genes/genética
Proteínas de Transporte
Estudos de Casos e Controles
Caderinas/genética
Carcinoma Ductal de Mama/patologia
Genes APC
GENES PABNORMALITIES, MULTIPLE
Hidrolases Anidrido Ácido/genética
Neoplasias da Mama/patologia
Proteínas Nucleares/genética
Proteínas de Neoplasias/genética
Reação em Cadeia da Polimerase/métodos
Limites: Adulto
Idoso
Idoso de 80 Anos ou mais
Feminino
Humanos
Masculino
Pessoa de Meia-Idade
Responsável: BR1.1 - BIREME


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Texto completo SciELO Brasil
Bonan, C. D
Battastini, A. M. O
Texto completo
Id: lil-224836
Autor: Emanuelli, T; Bonan, C. D; Sarkis, J. J. F; Battastini, A. M. O.
Título: Catabolism of Ap4A and Ap5A by rat brain synaptosomes
Fonte: Braz. j. med. biol. res = Rev. bras. pesqui. méd. biol;31(12):1529-32, Dec. 1998. graf.
Idioma: en.
Resumo: Adenosine 5',5'''-P1,P4-tetraphosphate (Ap4A) and adenosine 5',5'''-P1,P5-pentaphosphate (Ap5A) are stored in and released from rat brain synaptic terminals. In the present study we investigated the hydrolysis of dinucleotides (Ap4A and Ap5A) in synaptosomes from the cerebral cortex of adult rats. Ap4A and Ap5A, but not Ap3A, were hydrolyzed at pH 7.5 in the presence of 20 mM Tris/HCl, 2.0 mM MgCl2, 10 mM glucose and 225 mM sucrose at 37oC. The disappearance of the substrates measured by FPLC on a mono-Q HR column was both time and protein dependent. Since synaptosome integrity was at least 90 percent at the end of the assay, hydrolysis probably occurred by the action of an ecto-enzyme. Extracellular actions of adenine dinucleotides at central nervous system terminate due to the existence of ecto-nucleotidases which specifically cleave these dinucleotides. These enzymes in association with an ATP diphosphohydrolase and a 5'-nucleotidase are able to promote the complete hydrolysis of dinucleotides to adenosine in the synaptic cleft
Descritores: Hidrolases Anidrido Ácido/análise
Trifosfato de Adenosina/metabolismo
Córtex Cerebral/enzimologia
Fosfatos de Dinucleosídeos/metabolismo
Sinaptossomos/enzimologia
-Hidrolases Anidrido Ácido/fisiologia
Trifosfato de Adenosina/análise
Córtex Cerebral/química
Cromatografia Líquida de Alta Pressão
Ratos Wistar
Sinaptossomos/química
Limites: Masculino
Animais
Ratos
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME



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