Base de dados : LILACS
Pesquisa : D08.811.277.040.330.300.100.101 [Categoria DeCS]
Referências encontradas : 4 [refinar]
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Id: lil-775129
Autor: Chen, Chun; Xie, Tingna; Ye, Sudan; Jensen, Annette Bruun; Eilenberg, J ørgen.
Título: Selection of reference genes for expression analysis in the entomophthoralean fungus Pandora neoaphidis
Fonte: Braz. j. microbiol;47(1):259-265, Jan.-Mar. 2016. tab, graf.
Idioma: en.
Projeto: Natural Science Foundation of China; . International Foundation for Science; . Science and Technology Planning Project of Zhejiang Province; . Zhejiang Natural Science Foundation.
Resumo: Abstract The selection of suitable reference genes is crucial for accurate quantification of gene expression and can add to our understanding of host–pathogen interactions. To identify suitable reference genes in Pandora neoaphidis, an obligate aphid pathogenic fungus, the expression of three traditional candidate genes including 18S rRNA(18S), 28S rRNA(28S) and elongation factor 1 alpha-like protein (EF1), were measured by quantitative polymerase chain reaction at different developmental stages (conidia, conidia with germ tubes, short hyphae and elongated hyphae), and under different nutritional conditions. We calculated the expression stability of candidate reference genes using four algorithms including geNorm, NormFinder, BestKeeper and Delta Ct. The analysis results revealed that the comprehensive ranking of candidate reference genes from the most stable to the least stable was 18S (1.189), 28S (1.414) and EF1 (3). The 18S was, therefore, the most suitable reference gene for real-time RT-PCR analysis of gene expression under all conditions. These results will support further studies on gene expression in P. neoaphidis.
Descritores: Entomophthorales/genética
Genes Fúngicos
Perfilação da Expressão Gênica/métodos
Perfilação da Expressão Gênica/normas
Padrões de Referência
Reação em Cadeia da Polimerase em Tempo Real/métodos
Reação em Cadeia da Polimerase em Tempo Real/normas
-Fator 1 de Elongação de Peptídeos/genética
/genética
RNA, RIBOSOMAL, 1ABDOMINAL NEOPLASMSS/genética
/genética
RNA, RIBOSOMAL, ABORTION, HABITUALS/genética
Responsável: BR1.1 - BIREME


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Id: lil-723120
Autor: Lino, Zumaquero Rios José; Juventino, López-Tlacomulco José; Raúl, Rojas García; Estibaliz, Sansinenea.
Título: Lethal effects of a Mexican Beauveria bassiana (Balsamo) strain against Meccus pallidipennis (Stal)
Fonte: Braz. j. microbiol;45(2):551-557, Apr.-June 2014. ilus, graf.
Idioma: en.
Projeto: VIEP.
Resumo: The entomopathogenic fungus Beauveria bassiana (Balsamo 1835) Vuillemin is an effective alternative control agent against some agricultural pests and biological vectors of important diseases such as Chagas disease. In this work we studied an isolate of Beauveria bassiana from of the town of San Antonio Rayón, Puebla, Mexico and its entomopathogenic effects on Meccus pallidipennis (Stal 1872). Phylogenetic analysis using molecular comparison of the ITS and EF1α genes, showed that the resulting cladogram places the BUAP 04 strain with a relationship closer to the AFAO 9-6 strain, within the diversity of the B. bassiana sensu lato group. Although there was the possibility that BUAP 04 strain was a direct descendant of strains used in campaigns of biologic control, molecular study allowed us to recognize that it was a different fungus due to numerous inserts. A strain isolated from a T. dimiata was evaluated for pathogenicity against another triatoma (Meccus pallidipennis) species obtaining an LC50 of 4.16 x 10(6) spores/mL, confirming that the BUAP 04 strain is virulent for M. pallidipennis and could be a good prospect for formulations to control M. pallidipennis.
Descritores: Beauveria/crescimento & desenvolvimento
Triatoma/microbiologia
Triatoma/fisiologia
-Beauveria/classificação
Beauveria/genética
Beauveria/isolamento & purificação
Análise por Conglomerados
DNA Fúngico/química
DNA Fúngico/genética
DNA de Helmintos/química
DNA de Helmintos/genética
DNA Espaçador Ribossômico/química
DNA Espaçador Ribossômico/genética
México
Dados de Sequência Molecular
Filogenia
Fator 1 de Elongação de Peptídeos/genética
Controle Biológico de Vetores/métodos
Análise de Sequência de DNA
Análise de Sobrevida
Virulência
Limites: Animais
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


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Id: lil-709468
Autor: Shentu, Xuping; Zhan, Xiaohuan; Ma, Zheng; Yu, Xiaoping; Zhang, Chuanxi.
Título: Antifungal activity of metabolites of the endophytic fungus Trichoderma brevicompactum from garlic
Fonte: Braz. j. microbiol;45(1):248-254, 2014. ilus, graf, tab.
Idioma: en.
Projeto: National Natural Science Fundation of China; . Zhejiang Province Programs for Science and Technology Development; . Zhejiang Province Natural Science Fundation.
Resumo: The endophytic fungus strain 0248, isolated from garlic, was identified as Trichoderma brevicompactum based on morphological characteristics and the nucleotide sequences of ITS1-5.8SITS2 and tef1. The bioactive compound T2 was isolated from the culture extracts of this fungus by bioactivity-guided fractionation and identified as 4β-acetoxy-12,13-epoxy-Δ9-trichothecene (trichodermin) by spectral analysis and mass spectrometry. Trichodermin has a marked inhibitory activity on Rhizoctonia solani, with an EC50 of 0.25 µgmL-1. Strong inhibition by trichodermin was also found for Botrytis cinerea, with an EC50 of 2.02 µgmL-1. However, a relatively poor inhibitory effect was observed for trichodermin against Colletotrichum lindemuthianum (EC50 = 25.60 µgmL-1). Compared with the positive control Carbendazim, trichodermin showed a strong antifungal activity on the above phytopathogens. There is little known about endophytes from garlic. This paper studied in detail the identification of endophytic T. brevicompactum from garlic and the characterization of its active metabolite trichodermin.
Descritores: Antifúngicos/farmacologia
Endófitos/química
Alho/microbiologia
Trichoderma/química
Tricodermina/farmacologia
-Antifúngicos/isolamento & purificação
Botrytis/efeitos dos fármacos
Análise por Conglomerados
Colletotrichum/efeitos dos fármacos
DNA Fúngico/química
DNA Fúngico/genética
DNA Espaçador Ribossômico/química
DNA Espaçador Ribossômico/genética
DNA Ribossômico/química
DNA Ribossômico/genética
Endófitos/classificação
Endófitos/isolamento & purificação
Espectrometria de Massas
Testes de Sensibilidade Microbiana
Dados de Sequência Molecular
Filogenia
Fator 1 de Elongação de Peptídeos/genética
/genética
RNA, RIBOSOMAL, ABDOMEN.ABDOMINAL NEOPLASMSS/genética
Rhizoctonia/efeitos dos fármacos
Análise de Sequência de DNA
Trichoderma/classificação
Trichoderma/isolamento & purificação
Tricodermina/isolamento & purificação
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


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Id: lil-546328
Autor: Candido-Silva, J. A; Monesi, N.
Título: Bradysia hygida (Diptera, Sciaridae) presents two eukaryotic Elongation Factor 1A gene homologues: partial characterization of the eukaryotic Elongation Factor 1A-F1 gene
Fonte: Braz. j. med. biol. res = Rev. bras. pesqui. méd. biol;43(5):437-444, May 2010. ilus.
Idioma: en.
Resumo: Elongation factor 1A is a highly conserved protein that participates in translation. We report the occurrence of two genes homologous to the eukaryotic Elongation Factor 1A in Bradysia hygida and describe the partial cloning and characterization of the B. hygida eukaryotic Elongation Factor 1A-F1 (BheEF1A-F1) gene. The pattern of BheEF1A-F1 expression in the salivary gland at the end of the fourth larval instar was investigated using real-time PCR. The results showed that BheEF1A-F1 expression levels are relatively constant at the time when rapid changes in protein synthesis occur in this tissue. In situ hybridization experiments coupled to Southern blot analyses showed that the BheEF1A-F1 gene is located at position 3d of the A chromosome and a second gene homologous to eEF1A is located at position 6a of the X chromosome. Southern blot analyses showed that both the BheEF1A-F1 gene and the second gene homologous to eEF1A constitute non-amplified genes. The present results contribute to the molecular characterization of a sciarid eEF1A gene.
Descritores: Dípteros/genética
Genes de Insetos/genética
Fator 1 de Elongação de Peptídeos/genética
-Sequência de Bases
Southern Blotting
Larva/genética
Dados de Sequência Molecular
Reação em Cadeia da Polimerase Via Transcriptase Reversa
RNA Mensageiro/genética
Limites: Animais
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME



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