Base de dados : LILACS
Pesquisa : D08.811.277.151 [Categoria DeCS]
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Texto completo SciELO Brasil
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Id: lil-748237
Autor: Angelini, Leticia Mara Lima; Silva, Amanda Ribeiro Martins da; Rocco, Lucas de Freitas Coli; Milagre, Cintia Duarte de Freitas.
Título: A high-throughput screening assay for distinguishing nitrile hydratases from nitrilases
Fonte: Braz. j. microbiol;46(1):113-116, 05/2015. tab, graf.
Idioma: en.
Resumo: A modified colorimetric high-throughput screen based on pH changes combined with an amidase inhibitor capable of distinguishing between nitrilases and nitrile hydratases. This enzymatic screening is based on a binary response and is suitable for the first step of hierarchical screening projects.
Descritores: Aminoidrolases/análise
Colorimetria/métodos
Ensaios de Triagem em Larga Escala/métodos
Hidroliases/análise
-Amidoidrolases/antagonistas & inibidores
Concentração de Íons de Hidrogênio
Tipo de Publ: Estudos de Avaliação
Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


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Id: lil-607539
Autor: Dubey, Sachin; Singh, Amit; Banerjee, Uttam C.
Título: Response surface methodology of nitrilase production by recombinant Escherichia coli
Fonte: Braz. j. microbiol;42(3):1085-1092, July-Sept. 2011. ilus, tab.
Idioma: en.
Resumo: Growth and nitrilase production by recombinant Escherichia coli cells harbouring pET 21 (b) plasmid, for the expression of Pseudomonas putida nitrilase were improved using response surface methodology. Central composite design was used for obtaining ideal concentration of critical medium components which include fructose, tryptone, yeast extract and lactose. The optimal values for the concentration of fructose, tryptone, yeast extract and lactose were found to be 1.13, 2.26, 3.25 and 0.9 percent (w/v), respectively. Here, fructose served as carbon source for the growth while lactose was preferably used as inducer for the expression of foreign protein. Yeast extract in the medium was used as a growth promoter while tryptone was added as a major nitrogen source. Using this optimized medium, an experimental growth of 6.67 (OD at 600 nm) and nitrilase activity of 27.13 U/ml was achieved. This approach for medium development led to an enhancement of the growth and enzyme activity by 1.4 and 2.2 times, respectively, as compared to the un-optimized medium.
Descritores: Aminoidrolases/análise
Nitrilos/análise
Peptídeo Hidrolases/análise
Proteínas Recombinantes/análise
Proteínas de Escherichia coli/análise
-Catálise
Ativação Enzimática
Métodos
Técnicas
Tipo de Publ: Estudo Comparativo
Responsável: BR32.1 - Serviço de Biblioteca e Informação Biomédica


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Texto completo SciELO Chile
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Id: lil-518958
Autor: Feliú, María Susana; Perris, Paula Daniela; Slobodianik, Nora Haydeé.
Título: Adenosina deaminasa: su respuesta ante desequilibrios nutricionales / Adenosine deaminase in malnutrition
Fonte: Rev. chil. nutr;35(3):210-214, sept. 2008. graf.
Idioma: es.
Projeto: Universidad de Buenos Aires. Secretaría de Ciencia y Técnica.
Resumo: It has been previously shown that nutritional produces an increment in the activity of adenosine deaminase (ADA) in thymus, serum and other fluids in immunonocompromised patients. This study analyzed if ADA activity could be used as a biochemical indicator of nutritional status in populations at nutritional risk. Twenty six women with anorexia nervosa (AN) 14-32 years old, and 33 obese children (O) 5-13 years old were studied. ADA activity was determined as described by Giusty and Galante, comparing results with control subjects of the same age. The results (x +/- SD, expressed as IU/L) were AN: 25.7+8.2 and 21.0 +/- 4.6; and 0:27.1+9.1° and 23.0±5.6 in experimental and control individuals, respectively (p<0.02). These findings support the hypothesis that ADA activity in serum can be used as a functional indicator related to the defence mechanism in nutritional studies.

Se ha demostrado que el estrés nutricional provoca incremento en la actividad de adenosina deaminasa en timo de rata y además aumenta su actividad en el suero y otros fluidos biológicos de pacientes inmunocompro-metidos. En este estudio se analizó si la determinación de la actividad de ADA en suero podría considerarse parámetro bioquímico funcional en el seguimiento de poblaciones en riesgo nutricional. Se estudiaron mujeres con anorexia nerviosa (AN, 14-32 años) y niños obesos de ambos sexos (O, 5-13 años). La actividad de ADA se determinó por el método de Giusti y Galante. Los resultados se compararon con controles de igual edad. Los resultados (X +/- DE (UI/L)) fueron AN: 25.7 +/- 8.2 vs. 21.0 +/- 4.6 y O: 27.1 +/- 9.1vs. 23.0 +/- 5.6 (p<0.02). Estos hallazgos, avalarían la hipótesis surgida de estudios previos, de proponer la determinación de la actividad sérica de ADA, como un indicador funcional relacionado con los mecanismos de defensa en los estudios de nutrición.
Descritores: Aminoidrolases/metabolismo
Anorexia Nervosa/enzimologia
Obesidade/enzimologia
-Índice de Massa Corporal
Estudos de Casos e Controles
Chile
Biomarcadores
Estado Nutricional
Limites: Seres Humanos
Masculino
Feminino
Adolescente
Adulto
Responsável: CL1.1 - Biblioteca Central



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