Base de dados : LILACS
Pesquisa : D08.811.277.151.486.075 [Categoria DeCS]
Referências encontradas : 119 [refinar]
Mostrando: 1 .. 10   no formato [Detalhado]

página 1 de 12 ir para página                         

  1 / 119 LILACS  
              next record last record
seleciona
para imprimir
Fotocópia
Texto completo
Id: biblio-1283592
Autor: Adlat, Salah; Hayel, Farooq; Yang, Ping; Chen, Yang; Mar Oo, Zin; Zaw Myint, May Zun; Kumar Sah, Rajiv; Bahadar, Noor; Al-Azab, Mahmoud; Binta Bah, Fatoumata; Zheng, Yaowu; Feng, Xuechao.
Título: CRISPR-mediated base editing in mice using cytosine deaminase base editor 4
Fonte: Electron. j. biotechnol;52:59-66, July. 2021. ilus, tab.
Idioma: en.
Projeto: National Natural Science Foundation of China; . Natural Science Foundation of Jilin Province; . Science and Technology Project of Jilin Provincial Education Department.
Resumo: BACKGROUND: Many human genetic diseases arise from point mutations. These genetic diseases can theoretically be corrected through gene therapy. However, gene therapy in clinical application is still far from mature. Nearly half of the pathogenic single-nucleotide polymorphisms (SNPs) are caused by G:C>A:T or T:A>C:G base changes and the ideal approaches to correct these mutations are base editing. These CRISPR-Cas9-mediated base editing does not leave any footprint in genome and does not require donor DNA sequences for homologous recombination. These base editing methods have been successfully applied to cultured mammalian cells with high precision and efficiency, but BE4 has not been confirmed in mice. Animal models are important for dissecting pathogenic mechanism of human genetic diseases and testing of base correction efficacy in vivo. Cytidine base editor BE4 is a newly developed version of cytidine base editing system that converts cytidine (C) to uridine (U). RESULTS: In this study, BE4 system was tested in cells to inactivate GFP gene and in mice to introduce single-base substitution that would lead to a stop codon in tyrosinase gene. High percentage albino coat-colored mice were obtained from black coat-colored donor zygotes after pronuclei microinjection. Sequencing results showed that expected base changes were obtained with high precision and efficiency (56.25%). There are no off-targeting events identified in predicted potential off-target sites. CONCLUSIONS: Results confirm BE4 system can work in vivo with high precision and efficacy, and has great potentials in clinic to repair human genetic mutations.
Descritores: Adenosina Desaminase
Citosina
Sistemas CRISPR-Cas
Edição de Genes/métodos
-Sequência de Bases
Western Blotting
Modelos Animais
Reação em Cadeia da Polimerase em Tempo Real
Mutação
Limites: Animais
Camundongos
Responsável: CL1.1 - Biblioteca Central


  2 / 119 LILACS  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
Id: biblio-949502
Autor: Betancur, Carlos Alberto; Mejía, Mateo; Posada, Verônica.
Título: Meningitis por Listeria monocytogenes con ADA elevado en paciente inmunocompetente / Listeria monocytogenes meningitis in an immunocompetent patient with elevated ADA
Fonte: Acta méd. colomb;41(2):151-154, abr.-jun. 2016. tab.
Idioma: es.
Resumo: Resumen La neuroinfección por Listeria monocytogenes, que incluye meningitis y rombencefalitis, se ha descrito tradicionalmente en personas en los extremos de la vida (menores de un año, mayores de 50 años), embarazadas y pacientes inmunosuprimidos con disfunción de la inmunidad celular. Es poco frecuente el compromiso de pacientes jóvenes sin estas condiciones predisponentes. La elevación de la adenosin-deaminasa (ADA) en líquido cefalorraquídeo (LCR) se ha descrito como una prueba con alta sensibilidad y especificidad para el diagnóstico de meningitis tuberculosa, pero no en la causada por Listeria monocytogenes. En este artículo se presenta el caso de una paciente joven, sin inmunosupresión conocida, que presenta un cuadro subagudo de meningitis, LCR con pleocitosis de predominio mononuclear, parálisis del VI par craneal y elevación de ADA, secundario a compromiso por L. monocytogenes. (Acta Med Colomb 2016; 41: 151-154).

Abstract Neuroinfection by Listeria monocytogenes, which includes meningitis and rhombencephalitis, has traditionally been described in people at the extremes of life (under one year and over 50 years), pregnant women and immunosuppressed patients with dysfunction of cellular immunity. The compromise of young patients without these predisposing conditions is rare. The elevation of the adenosine deaminase (ADA) in cerebrospinal fluid (CSF) has been described as a test with high sensitivity and specificity for the diagnosis of tuberculous meningitis, but not for the meningitis caused by Listeria monocytogenes. The case of a young female patient with no known immunosuppression who presents a clinical picture of subacute meningitis, CSF with mononuclear pleocytosis dominance, VI cranial nerve paralysis and elevated ADA secondary to L. monocytogenes compromise is presented. (Acta Med Colomb 2016; 41: 151-154).
Descritores: Meningite
-Adenosina Desaminase
Encefalite
Listeria monocytogenes
Limites: Humanos
Feminino
Adulto
Tipo de Publ: Relatos de Casos
Responsável: CO70 - Asociación Colombiana de Medicina Interna


  3 / 119 LILACS  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
Id: biblio-1045760
Autor: Marie, MAM; John, J; Krishnappa, L Gowda; Gopalkrishnan, S; Bindurani, SR; CS, P.
Título: Role of interleukin-6, gamma interferon and adenosine deaminase markers in management of pleural effusion patients / Papel de la interleucina-6, el interferón gamma, y la adenosina desaminasa como marcadores en el tratamiento de pacientes con derrame pleural
Fonte: West Indian med. j;62(9):803-807, Dec. 2013. tab.
Idioma: en.
Projeto: King Saud University. Deanship of Scientific Research.
Resumo: OBJECTIVE: Pleural effusion is a common diagnostic and clinical problem. Neoplasms and tuberculosis are the most frequent diagnostic causes of such effusions. Conventional laboratory methods for diagnosis of such effusion are inefficient because tubercle bacilli are rarely seen in direct examinations of pleural fluid. The present study evaluates interleukin-6 (IL-6), gamma interferon (IFN-γ) and adenosine deaminase (ADA) as diagnostic tools in pleural effusion. METHODS: Interleukin-6, IFN-γ and ADA were measured in pleural fluid from the patients, with exudative pleural effusion from tuberculous, malignant and postpneumonic origin and transudative pleural effusion ofsystemic origin in order to evaluate the diagnostic utility ofthese. RESULTS: The three markers were detectable in all effusions with significantly high levels in exudative as compared to transudative effusions. There was a statically significant difference noticed in tuberculous as compared to malignant andpostpneumonic origin and transudative pleural effusion. CONCLUSION: We concluded that IL-6, IFN-γ and ADA levels in pleural effusion are sensitive parameters to differentiate an exudate from a transudate and they can also differentiate exudates of different aetiology. Finally, the results suggest that there is a remarkable difference in production of these three markers in exudative pleural effusions as compared to transudative pleural effusions.

OBJETIVO: El derrame pleural es un problema diagnóstico y clínico común. Las neoplasias y la tuberculosis son las causas más frecuentes en los diagnósticos de tales derrames. Los métodos de laboratorio convencionales para el diagnóstico de tales derrames son ineficientes, porque los bacilos de la tuberculosis raramente se ven en los exámenes directos del líquido pleural. El presente estudio evalúa la interleucina-6, el interferón gamma (IFN-γ) y la adenosina desaminasa (ADA) como herramientas de diagnóstico en el derrame pleural. MÉTODOS: La interleucina-6, el IFN-γ, y la ADA fueron medidos en el líquido pleural de los pacientes con derrame pleural exudativo de origen tuberculoso, maligno y post-pneumónico, y el derrame pleural trasudativo de origen sistémico, con el fin de evaluar la utilidad diagnóstica de éstos. RESULTADOS: Los tres marcadores eran observables en todos los derrames, con niveles significativamente altos en los exudativos en comparación con los trasudativos. Se notó una diferencia estadísticamente significativa en los derrames de origen tuberculoso en comparación con los de origen maligno y postpneumónico, y los derrames pleurales trasudativos. CONCLUSIÓN: Llegamos a la conclusión de que los niveles de IL-6, IFN-Correspondence: Dr M Marie, College of Applied Medical Sciences, Clinical Laboratory Department, King Saud University, PO Box 10219, Riyadh 11433, Kingdom of Saudi Arabia. E-mail: drmmarie.2000@ gmail.com *Equally contributed to the manuscript YADA en el derrame pleural, son parámetros sensibles para diferenciar un derrame pleural exudado de uno trasudado, pudiendo por otra parte ayudar también a distinguir exudados de diferentes etiologías. Finalmente, los resultados sugieren que existe una diferencia notable en la forma en que se producen estos tres marcadores en los derrames efusiones pleurales exudativos en comparación con los derrames pleurales trasudativos.
Descritores: Derrame Pleural/diagnóstico
Adenosina Desaminase/análise
Interleucina-6/análise
Interferon gama/análise
Exsudatos e Transudatos/química
-Biomarcadores/análise
Limites: Humanos
Tipo de Publ: Estudo Comparativo
Responsável: BR1.1 - BIREME


  4 / 119 LILACS  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo SciELO Brasil
Texto completo
Id: biblio-1249577
Autor: Ren, Zenghua; Xu, Ling.
Título: Role of cancer ratio and other new parameters in the differential diagnosis of malignant pleural effusion
Fonte: Clinics;76:e2515, 2021. tab.
Idioma: en.
Resumo: OBJECTIVES: We compared the diagnostic potential of cancer ratio (CR, serum lactate dehydrogenase [LDH]/pleural fluid adenosine deaminase [pfADA]), cancer ratio plus (CR plus, cancer ratio/pleural lymphocyte percentage), and age/pfADA ratio with pfADA in malignant pleural effusion. METHODS: Data from 100 patients with malignant pleural effusion (MPE) and 119 patients with tuberculous pleural effusion (TPE) were retrospectively collected. PfADA, age/pfADA ratio, CR, and CR plus were compared between patients with MPE and those with TPE in two age groups (≤50 and >50 years). The best cut-off value was determined, and the diagnostic performance was evaluated according to the receiver operating characteristic curve. RESULTS: PfADA was statistically significantly lower while age/pfADA ratio, CR, and CR plus were significantly higher in the MPE group than in the TPE group in both age groups (p<0.05). For patients aged ≤50 years, the differential diagnostic value of pfADA for MPE was better than those of age/pfADA ratio, CR, and CR plus. At a cut-off value of 13.0 U/L, the sensitivity, specificity, and accuracy were 88.9%, 100.0%, and 98.9%, respectively. For patients aged >50 years, the diagnostic performance of CR plus was superior to those of pfADA, age/pfADA ratio, and CR. At a cut-off value of 22.6, the sensitivity, specificity, and accuracy of CR plus for the diagnosis of MPE were 86.8%, 84.6%, and 86.2%, respectively. CONCLUSIONS: The best parameter for diagnosing MPE was different for patients aged ≤50 years and >50 years. For patients aged >50 years, CR plus was a good parameter for the differential diagnosis of MPE. For patients aged ≤50 years, pfADA was better.
Descritores: Derrame Pleural/diagnóstico
Derrame Pleural Maligno/diagnóstico
-Adenosina Desaminase
Estudos Retrospectivos
Sensibilidade e Especificidade
Diagnóstico Diferencial
Limites: Humanos
Pré-Escolar
Pessoa de Meia-Idade
Responsável: BR1.1 - BIREME


  5 / 119 LILACS  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
Id: biblio-1129025
Autor: Oyonarte W, Maite.
Título: Enfoque diagnóstico en el paciente con derrame pleural / Diagnostic approach in pleural effusion
Fonte: Rev. Méd. Clín. Condes;26(3):313-324, mayo 2015. ilus, tab.
Idioma: es.
Resumo: En el estudio diagnóstico del paciente con derrame pleural se deben considerar la historia clínica y el análisis de las imágenes para acotar el diagnóstico diferencial. El uso adecuado de las técnicas de imágenes contribuye a realizar procedimientos en forma segura. Se debe realizar una toracocentesis diagnóstica y/o evacuadora y se debe analizar completamente el líquido pleural. A veces es necesario realizar biopsia pleural para lo cual existen diversas técnicas disponibles. En los pacientes con pleuritis crónica inespecífica se debe hacer seguimiento por dos años para evaluar el desarrollo de malignidad.

The diagnostic approach in patients with pleural effusion must begin considering clinical aspects and image interpretation. Different imaging techniques can safely guide invasive procedures. Diagnostic or therapeutic thoracentesis must be performed and pleural fluid must be completely analyzed. Some patient will require pleural biopsy, and different techniques are available. Patients with chronic unspecific pleuritis histological diagnosis after pleural biopsy, must be followed for two years long to be sure no malignancy is developed.
Descritores: Derrame Pleural/diagnóstico
-Derrame Pleural/classificação
Derrame Pleural/etiologia
Derrame Pleural/microbiologia
Derrame Pleural/diagnóstico por imagem
Toracoscopia
Biópsia
Biomarcadores
Adenosina Desaminase/análise
Diagnóstico Diferencial
Exsudatos e Transudatos
Toracentese
Concentração de Íons de Hidrogênio
Limites: Humanos
Responsável: CL1.1 - Biblioteca Central


  6 / 119 LILACS  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
Id: biblio-999237
Autor: Rodríguez, Rubén.
Título: Tuberculosis pleural en paciente pediátrico: reporte de un caso y revisión de la literatura / Pleural tuberculosis in a pediatric patient: case report and review
Fonte: Neumol. pediátr. (En línea);13(1):29-31, ene. 2018. ilus.
Idioma: es.
Resumo: Tuberculosis (TB) is a common cause of pleural effusion in young people from endemic areas. Among the forms of extrapulmonary TB in people with immunodeficiencies, the most frequent localization is the pleura. The use of immunological and molecular biology tests for the diagnosis of TB in pleural fluid and other locations with high sensitivity and specificity is highlighted. We present a clinical case with the objective of giving an overview of the treatment of the patient with suspected pleural tuberculosis

La Tuberculosis (TB) es una causa común de derrame pleural en jóvenes en zonas endémicas. Dentro de las formas de TB extrapulmonar en personas que cursan con inmunodeficiencias, la localización más frecuente es la TB pleural. Se destaca el uso de las pruebas inmunológicas y de biología molecular para el diagnóstico de TB en líquido pleural y de otras localizaciones con una elevada sensibilidad y especificidad. Se presenta un caso clínico con el objetivo de describir una visión general del abordaje del paciente con sospecha de tuberculosis pleural
Descritores: Derrame Pleural/etiologia
Tuberculose Pleural/complicações
Tuberculose Pleural/diagnóstico
-Derrame Pleural/enzimologia
Tuberculose Pleural/enzimologia
Tuberculose Pleural/diagnóstico por imagem
Radiografia Torácica
Tomografia Computadorizada por Raios X
Adenosina Desaminase
Limites: Humanos
Feminino
Adolescente
Responsável: CL1.1 - Biblioteca Central


  7 / 119 LILACS  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo SciELO Chile
Texto completo
Id: biblio-983940
Autor: Sagredo, Eduardo A; Blanco, Alejandro; Sagredo, Alfredo I; Pérez, Paola; Sepúlveda-Hermosilla, Gonzalo; Morales, Fernanda; Müller, Bettina; Verdugo, Ricardo; Marcelain, Katherine; Harismendy, Olivier; Armisén, Ricardo.
Título: ADAR1-mediated RNA-editing of 3'UTRs in breast cancer
Fonte: Biol. Res;51:36, 2018. graf.
Idioma: en.
Projeto: FONDECYT; . NCI-Leidos; . NIH; . Anillo en Ciencia y Tecnología; . FONDEF; . CORFO.
Resumo: BACKGROUND: Whole transcriptome RNA variant analyses have shown that adenosine deaminases acting on RNA ( ADAR ) enzymes modify a large proportion of cellular RNAs, contributing to transcriptome diversity and cancer evolution. Despite the advances in the understanding of ADAR function in breast cancer, ADAR RNA editing functional consequences are not fully addressed. RESULTS: We characterized A to G(I) mRNA editing in 81 breast cell lines, showing increased editing at 3'UTR and exonic regions in breast cancer cells compared to immortalized non-malignant cell lines. In addition, tumors from the BRCA TCGA cohort show a 24% increase in editing over normal breast samples when looking at 571 well-characterized UTRs targeted by ADAR1. Basal-like subtype breast cancer patients with high level of ADAR1 mRNA expression shows a worse clinical outcome and increased editing in their 3'UTRs. Interestingly, editing was particularly increased in the 3'UTRs of ATM, GINS4 and POLH transcripts in tumors, which correlated with their mRNA expression. We confirmed the role of ADAR1 in this regulation using a shRNA in a breast cancer cell line (ZR-75-1). CONCLUSIONS: Altogether, these results revealed a significant association between the mRNA editing in genes related to cancer-relevant pathways and clinical outcomes, suggesting an important role of ADAR1 expression and function in breast cancer.
Descritores: Neoplasias da Mama/genética
Adenosina Desaminase/genética
Proteínas de Ligação a RNA/genética
Edição de RNA/genética
Regiões não Traduzidas/genética
Estabilidade de RNA/genética
-Neoplasias da Mama/metabolismo
Regulação Neoplásica da Expressão Gênica
Adenosina Desaminase/metabolismo
Proteínas de Ligação a RNA/metabolismo
Perfilação da Expressão Gênica
Estabilidade de RNA/fisiologia
Linhagem Celular Tumoral
Limites: Humanos
Feminino
Responsável: CL1.1 - Biblioteca Central


  8 / 119 LILACS  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
Id: biblio-1129050
Autor: Porcel, José Manuel.
Título: Diagnóstico diferencial del derrame pleural a través de los valores de corte de los parámetros bioquímicos del líquido / Differential diagnosis of pleural effusion assessed by cut off values of biochemical pleural fluid tests
Fonte: Salud(i)ciencia (Impresa) = Salud(i)ciencia (En linea);14(6):392-396, sept. 2006.
Idioma: es.
Resumo: Objective: The aim of biochemical pleural fluid testing is to reach an etiological diagnosis of the pleural effusion. We assessed the utility of considering cut-off points for the wide range of analyses used to investigate pleural fluid. Patients and methods: Among 1 586 patients with pleural effusion, we sought the etiologies of those fluids which showed any of the following characteristics: red blood cell count ≥ 10 x 109 l, leukocytes ≥ 10 x 109 /l, percentage of neutrophils or lymphocytes > 50%, protein ≥ 30 and 50 g/l, glucose ≤ 60 mg/dl, pH ≤ 7.20, lactate dehydrogenase ≥ 1 000 U/l, adenosine deaminase ≥ 40 U/l, amylase ≥ 100 U/l or cholesterol ≥ 40 and 60 mg/dl. Results: Some of the more prominent findings were: 1) 15% and 18% of transudates were blood-tinged or contained predominantly neutrophils respectively, 2) a grossly bloody fluid suggests malignant disease, trauma, pulmonary embolization or pneumonia, 3) nearly 80% of fluids containing > 10 x 109 / leukocytes/l were parapneumonics, 4) 72% of tuberculous pleural fluids had protein > 50 g/l, 5) tuberculosis and parapneumonics explained 90% of fluids with high adenosine deaminase content, 6) one third of amylase-rich pleural effusions were malignant, 7) a low pleural glucose or pH levels indicates a parapneumonic and, less frequently a tuberculous or malignant etiology, 8) the overall diagnostic yield of pleural fluid cytology in malignant effusions was 61%, a percentage which exceeded 80% in those with low pH or glucose fluid levels. Conclusions: Cut-off values of biochemical pleural fluid

Objetivo: Evaluar la utilidad de diversos valores de corte aplicados a una gran variedad de parámetros bioquímicos del líquido pleural para identificar la causa de un derrame pleural. Pacientes y métodos: En 1 586 pacientes con derrame pleural se analizaron las causas de aquellos líquidos que presentaban alguna de las siguientes características: hematíes ≥ 10 x 109/l, leucocitos ≥ 10 x 109/l, porcentaje de neutrófilos o linfocitos > 50%, proteínas ≥ 30 y 50 g/l, glucosa ≤ 60 mg/dl, pH ≤ 7.20, lactato deshidrogenasa ≥ 1 000 U/l, adenosina desaminasa ≥ 40 U/l, amilasa ≥ 100 U/l o colesterol ≥ 45 y 60 mg/dl. Resultados: Algunos de los hallazgos más destacados fueron: 1) 15% y 18% de los trasudados eran sanguinolentos o tenían celularidad de predominio neutrofílico, respectivamente; 2) un derrame francamente hemático sugiere malignidad, traumatismo, embolia pulmonar o neumonía; 3) cerca del 80% de muestras de líquidos con más de 10 x 109 leucocitos/l eran paraneumónicas; 4) el 72% de los líquidos tuberculosos tenían cifras de proteínas ≥ 50 g/l; 5) los derrames tuberculosos y paraneumónicos explican el 90% de los líquidos con concentraciones elevadas de adenosina desaminasa; 6) un tercio de los derrames ricos en amilasa son malignos; 7) cifras bajas de glucosa o pH pleurales indican que el paciente probablemente tiene un derrame paraneumónico o, en menos ocasiones, tuberculoso o maligno; 8) la rentabilidad global de la citología en los derrames malignos fue del 61%, porcentaje que superó el 80% en los líquidos con concentraciones bajas de pH o glucosa. Conclusiones: La consideración de determinados valores de corte de los parámetros bioquímicos del líquido pleural puede apoyar fuertemente algunas etiologías de derrame pleural
Descritores: Derrame Pleural
Adenosina
Adenosina Desaminase
Lactato Desidrogenases
L-Lactato Desidrogenase
Limites: Humanos
Tipo de Publ: Relatório Técnico
Responsável: AR392.1 - Biblioteca


  9 / 119 LILACS  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Id: lil-176288
Autor: Rojas, Bertha; Yi, Augusto; Accinelli, Roberto.
Título: Adenosina deaminasa (ADA) en el diagnóstico de tuberculosis pleural / Adenosine deaminase in the diagnosis of pleural tuberculosis
Fonte: Rev. méd. hered;4(3):115-8, sept. 1993. ilus.
Idioma: es.
Resumo: Se estudiaron los valores de adenosin deaminasa (ADA) en 32 pacientes con exudado peural, cuya etiología se determinó por otros medios. A todos se le practicó biopsia pleural percutánea. Veintitres fueron varones (74.2 por ciento). Veinte pacientes tuvieron tuberculosis pleural, 7 tenían neoplasia y 5 otra causa. Los valores de ADA fueron de 230.65 U/L, 53.85 y 78.4 respectivamente. Utilizando 95 U/L de ADA como punto de corte para el diagnóstico de tuberculosis se encontró una sensibilidad del 100 por ciento y una especificidad del 75 por ciento
Descritores: Adenosina Desaminase
-Derrame Pleural/diagnóstico
Derrame Pleural/etiologia
Tuberculose Pleural/diagnóstico
Tuberculose Pleural/etiologia
Limites: Humanos
Responsável: PE1.1 - Oficina Universitária de Biblioteca


  10 / 119 LILACS  
              first record previous record
seleciona
para imprimir
Fotocópia
Texto completo
Id: lil-609514
Autor: Quiñones-Silva, Jhonatan Boris; Ramírez-Yépez, César Héctor Gonzalo; Peña-Oscuvilca, Américo; Estrada-Choque, Efraín.
Título: Validez de la prueba de adenosina deaminasa y del recuento diferencial de leucocitos para el diagnóstico de tuberculosis pleural / Validity of both adenosine deaminase test and differential cell count for pleural tuberculosis diagnosis
Fonte: An. Fac. Med. (Perú);71(1):18-22, ene.-mar. 2010. tab, graf.
Idioma: es.
Resumo: Objetivo: Determinar la validez de la prueba de adenosina deaminasa (ADA) y el recuento diferencial de leucocitos para el diagnóstico de tuberculosis pleural. Diseño: Estudio retrospectivo de precisión diagnóstica. Institución: Hospital Regional de Huacho, Ministerio de Salud, Huacho, Perú. Material: Historias clínicas de pacientes con prueba de ADA en líquido pleural. Intervenciones: Estudio de historias clínicas de pacientes a quienes se les realizó la prueba de ADA en líquido pleural, desde enero 2005 hasta diciembre 2007. El valor de la prueba de ADA y el recuento diferencial de leucocitos fueron analizados mediante la curva ROC. La sensibilidad (Se), especificidad (Sp), valores predictivos (VPP y VPN) y los cocientes de verosimilitud (CVP y CVN) fueron hallados para cada variable. Principales medidas de resultados: Punto de corte para la prueba de ADA para el diagnóstico de tuberculosis pleural. Resultados: La tuberculosis fue culpable de la mayoría de los derrames pleurales; 47,5 fue el punto de corte hallado para la prueba de ADA, con Se y Sp de 73 por ciento y 90 por ciento, respectivamente (IC 95 por ciento: 0,74 - 0,95; p<0,001). La curva ROC para el recuento diferencial de leucocitos dio un punto de corte de 0,725; con Se y Sp de 60,9 y 64,7, respectivamente (IC 95 por ciento: 0,47 - 0,79; p=0,129). Conclusiones: El mejor punto de corte discriminatorio para el diagnóstico de tuberculosis pleural fue 47,5 UI/L. Esta prueba sirve más para confirmar el diagnóstico que para descartar la enfermedad. Además, un mayor porcentaje de linfocitos en el recuento diferencial de leucocitos (>72 por ciento) incrementaría la sospecha de tuberculosis pleural.

Objective: To determine the validity of both adenosine diaminase (ADA) test and differential cell count for pleural tuberculosis diagnosis. Design: Retrospective study for diagnosis precision. Setting: Huacho Hospital, Ministerio de Salud, Huacho, Peru. Material: Medical records of patients with ADA test in pleural fluid. Interventions: Medical records study of patients with ADA test in pleural effusion performed from January 2005 through December 2007. ADA test values and differential cell counts were analyzed by ROC curve. Sensitivity (Se), specificity (Sp), predictive values (VPP and VPN) and likehood ratios (LRP and LRN) were found for each variable. Main outcome measures: ADA test cut-off point for pleural tuberculosis diagnosis. Results: Tuberculosis was responsible for most cases of pleural effusion; 47,5 UI/L was the cut-off point by ROC curve, with Se and Sp 73 per cent and 90 per cent, respectively (IC 95 per cent: 0,74 - 0,95; p<0,001). The ROC curve for differential cell count cut-off point was 0,725, with Se and Sp 60,9 and 64,7, respectively (IC 95 per cent: 0,47 - 0,79; p=0,129). Conclusions: The best cut-off point for pleural tuberculosis diagnosis was 47,5 UI/L. This test worked better to confirm the disease rather than ruling it out. Thus, a larger percentage of lymphocytes in the differential cell count (>72 per cent) will increase the suspicious of pleural tuberculosis.
Descritores: Adenosina Desaminase
Contagem de Leucócitos
Tuberculose Pleural
-Estudos Retrospectivos
Limites: Humanos
Responsável: PE13.1 - Oficina de Biblioteca, Hemeroteca y Centro de Documentación



página 1 de 12 ir para página                         
   


Refinar a pesquisa
  Base de dados : Formulário avançado   

    Pesquisar no campo  
1  
2
3
 
           



Search engine: iAH v2.6 powered by WWWISIS

BIREME/OPAS/OMS - Centro Latino-Americano e do Caribe de Informação em Ciências da Saúde