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Pesquisa : D08.811.277.352.100.400 [Categoria DeCS]
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Id: biblio-1157399
Autor: Tiscornia Osvaldo Manuel; Rodríguez Ricardo Raúl; Sussemil Carlota; Otero Graciela; Negri Gustavo Alberto; Waisman Hipólito; López Mingorance Fabiana Norma; Tiscornia Wasserman Patricia Graciela.
Título: Streptozotocin-induced diabetes, bile-pancreatic secretion and insulo-pancreon-axis interaction / Streptozotocin-induced diabetes, bile-pancreatic secretion and insulo-pancreon-axis interaction.
Fonte: Acta gastroenterol. latinoam;43(4):294-300, 2013 Dec.
Idioma: es.
Resumo: The present tests were undertaken in order to analyze in male Wistar rats the changes in the exocrine and endocrine pancreas and on the interactions that normally evolve in the insulo-pancreon-axis. To evaluate this by a single i.p. Boots secretin injection, glycemia (G), amylasemia (A) and lipasemia (L) were determined. In bile-pancreatic secretion, we analyzed, pre and post-secretin, the following parameters: volume (V), bicarbonate output (BO), amylase output (AO) and lipase output (LO). Three groups of tests were done: a) control (C); b) streptozotocin-treated non-diabetic-rats (St-ND) and c) streptozotocin-treated diabetic animals (St-D) which showed morning glycemia values higher than 16.0 mmol/l. Four months later, under Tiopental i.p anesthesia, a bile-pancreatic fistula was done. Following a 30 min basal period, Boots secretin (20 CU/kg) was i.p injected. Bile-pancreatic secretion put in evidence a significant fall of BO in both St-ND and St-D series. In controls, AO revealed a post-secretin increase of 160

, while in the St-D rats showed a depression of 41

. The behavior of L was different, being augmented (+27

) in the C, while in the St-D rats the response was significantly higher (+95

). In bile-pancreatic-secretion, the fall of BO and AO in the St-ND and St-D series in respect to the C, are probably consequence of the diminishing potentiating effects exerted normally by insulin on the secretin-induced water and bicarbonate secretion of the pancreon units. In contrast, the rising of LO in the St-D, an expression of an enhancing pancreocyte's synthesis and secretion of lipase. The blood changes of A (depression) and of L (increase) in respect to the C values, although without reaching significant level, mirror those observed in bile-pancreatic secretion.
Descritores: Bile/metabolismo
Diabetes Mellitus Experimental/metabolismo
Pâncreas/metabolismo
-Amilases/metabolismo
Animais
Diabetes Mellitus Experimental/enzimologia
Estreptozocina
Lipase/metabolismo
Masculino
Ratos
Ratos Wistar
Secretina/metabolismo
Tipo de Publ: Artigo de Revista
Responsável: AR5.1 - Centro de Gestión del Conocimiento y las Comunicaciónes


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Id: biblio-1052260
Autor: Huang, Lin; Zheng, Dong; Zhao, Yatong; Ma, Jieying; Li, Yanzhen; Xu, Zehua; Shan, Mengying; Shao, Shulin; Guo, Qingwen; Zhang, Jie; Lu, Fuping; Liu, Yihan.
Título: Improvement of the alkali stability of Penicillium cyclopium lipase by error-prone PCR
Fonte: Electron. j. biotechnol;39:91-97, may. 2019. ilus, graf, tab.
Idioma: en.
Projeto: National Key R&D Program of China; . National Natural Science Foundation of China; . China Postdoctoral Science Foundation; . Natural Science Foundation of Tianjin City.
Resumo: BACKGROUND: Lipases are extensively exploited in lots of industrial fields; cold-adapted lipases with alkali-resistance are especially desired in detergent industry. Penicillium cyclopium lipase I (PCL) might be suitable for applications of detergent industry due to its high catalytic efficiency at low temperature and relatively good alkali stability. In this study, to better meet the requirements, the alkali stability of PCL was further improved via directed evolution with error-prone PCR. RESULTS: The mutant PCL (N157F) with an improved alkali stability was selected based on a high-throughput activity assay. After incubating at pH 11.0 for 120 min, N157F retained 70% of its initial activity, which was 23% higher than that of wild type PCL. Combined with the three-dimensional structure analysis, N157F exhibited an improved alkali stability under the high pH condition due to the interactions of hydrophilicity and ß-strand propensity. Conclusions: This work provided the theoretical foundation and preliminary data for improving alkali stability of PCL to meet the industrial requirements, which is also beneficial to improving alkali-tolerance ability of other industrial enzymes via molecular modification.
Descritores: Penicillium/enzimologia
Estabilidade Enzimática
Indústria de Detergentes
Lipase/metabolismo
-Penicillium/isolamento & purificação
Penicillium/genética
Reação em Cadeia da Polimerase/métodos
Temperatura Baixa
Álcalis
Biocatálise
Interações Hidrofóbicas e Hidrofílicas
Concentração de Íons de Hidrogênio
Lipase/isolamento & purificação
Lipase/genética
Mutação
Responsável: CL1.1 - Biblioteca Central


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Id: biblio-1052027
Autor: Delgado-García, Mariana; Flores-Gallegos, Adriana C; Kirchmayr, Manuel; Rodríguez, Jorge A; Mateos-Díaz, Juan C; Aguilar, Cristobal N; Muller, Marcelo; Camacho-Ruíz, Rosa M.
Título: Bioprospection of proteases from Halobacillus andaensis for bioactive peptide production from fish muscle protein
Fonte: Electron. j. biotechnol;39:52-60, may. 2019. ilus, tab, graf.
Idioma: en.
Resumo: BACKGROUND: Biologically active peptides produced from fish wastes are gaining attention because their health benefits. Proteases produced by halophilic microorganisms are considered as a source of active enzymes in high salt systems like fish residues. Hence, the aim of this study was the bioprospection of halophilic microorganisms for the production of proteases to prove their application for peptide production. RESULTS: Halophilic microorganisms were isolated from saline soils of Mexico and Bolivia. An enzymatic screening was carried out for the detection of lipases, esterases, pHB depolymerases, chitinases, and proteases. Most of the strains were able to produce lipases, esterases, and proteases, and larger hydrolysis halos were detected for protease activity. Halobacillus andaensis was selected to be studied for proteolytic activity production; the microorganism was able to grow on gelatin, yeast extract, skim milk, casein, peptone, fish muscle (Cyprinus carpio), and soy flour as protein sources, and among these sources, fish muscle protein was the best inducer of proteolytic activity, achieving a protease production of 571 U/mL. The extracellular protease was active at 50°C, pH 8, and 1.4 M NaCl and was inhibited by phenylmethylsulfonyl fluoride. The proteolytic activity of H. andaensis was used to hydrolyze fish muscle protein for peptide production. The peptides obtained showed a MW of 5.3 kDa and a radical scavenging ability of 10 to 30% on 2,2-diphenyl-1-picrylhydrazyl and 2,2-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) and a ferric reducing ability of plasma. Conclusion: The use of noncommercial extracellular protease produced by H. andaensis for biologically active peptide production using fish muscle as the protein source presents a great opportunity for high-value peptide production.
Descritores: Peptídeo Hidrolases/metabolismo
Peptídeos/metabolismo
Proteínas de Peixes/metabolismo
Halobacillus/enzimologia
-Solo
Bactérias/isolamento & purificação
Bolívia
Esterases
Salinidade
Hidrólise
Lipase
México
Proteínas Musculares
Antioxidantes
Responsável: CL1.1 - Biblioteca Central


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Id: biblio-1128481
Autor: Decarlini, María Florencia.
Título: La hipertrigliceridemia leve a moderada parece incrementar también el riesgo de pancreatitis aguda / Mild-to-moderate hypertrigliceridemia may increase the risk of acute pancreatitis
Fonte: Evid. actual. práct. ambul;20(2):56-56, 2017. tab.
Idioma: es.
Descritores: Pancreatite/complicações
Hipertrigliceridemia/complicações
-Pâncreas/metabolismo
Triglicerídeos/sangue
Índice de Gravidade de Doença
Risco
Estudos de Coortes
Dinamarca
Amilases/metabolismo
Lipase/sangue
Infarto do Miocárdio/complicações
Limites: Humanos
Masculino
Feminino
Adulto
Pessoa de Meia-Idade
Idoso
Adulto Jovem
Tipo de Publ: Comentário
Responsável: AR2.1 - Biblioteca Central


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Texto completo SciELO Brasil
Texto completo
Id: lil-757366
Autor: Bolsoni-Lopes, Andressa; Alonso-Vale, Maria Isabel C.
Título: Lipolysis and lipases in white adipose tissue – An update
Fonte: Arch. endocrinol. metab. (Online);59(4):335-342, Aug. 2015. ilus.
Idioma: en.
Resumo: Lipolysis is defined as the sequential hydrolysis of triacylglycerol (TAG) stored in cell lipid droplets. For many years, it was believed that hormone-sensitive lipase (HSL) and monoacylglycerol lipase (MGL) were the main enzymes catalyzing lipolysis in the white adipose tissue. Since the discovery of adipose triglyceride lipase (ATGL) in 2004, many studies were performed to investigate and characterize the actions of this lipase, as well as of other proteins and possible regulatory mechanisms involved, which reformulated the concept of lipolysis. Novel findings from these studies include the identification of lipolytic products as signaling molecules regulating important metabolic processes in many non-adipose tissues, unveiling a previously underestimated aspect of lipolysis. Thus, we present here an updated review of concepts and regulation of white adipocyte lipolysis with a special emphasis in its role in metabolism homeostasis and as a source of important signaling molecules.
Descritores: Tecido Adiposo Branco/enzimologia
Lipase/metabolismo
Lipólise/fisiologia
-Tecido Adiposo Branco/fisiologia
Lipase/fisiologia
Limites: Humanos
Tipo de Publ: Revisão
Responsável: BR1.1 - BIREME


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Id: lil-706161
Autor: Marques, Simone Cristina; Evangelista, Suzana Reis; Piccoli, Roberta Hilsdorf.
Título: Diversidade e resistência a antibióticos de bactérias psicrotróficas isoladas de tanques coletivos de resfriamento de leite / Diversity of psychotrophic bacteria isolated from the milk cooling collective tanks and antibiotics resistance profile of the microorganisms isolates
Fonte: Rev. Inst. Adolfo Lutz;71(4):670-676, out.-dez. 2012. tab, graf.
Idioma: pt.
Resumo: Bactérias psicrotróficas isoladas da parede, do fundo e da pá de homogeneização de tanques coletivos de resfriamento de leite, após o processo de higienização, foram identificadas e caracterizadas quanto à capacidade de produzir lipase, lecitinas e e protease, bem como determinada a sensibilidade dos isolados a antibióticos. Foram coletadas amostras de 32 tanques coletivos, localizados em diferentes cidades no sul do estado de Minas Gerais, por meio de swab de amostra da superfície, e analisadas pelas técnicas de diluições seriadas com plaqueamento em ágar triptona de soja e incubação das placas a 7 ºC por10 dias. As bactérias isoladas foram identificadas bioquimicamente pelos kits API 20NE (Biomérieux-Brasil) e Bactray I e II (Laborclin-Brasil). Foram identificados 197 isolados, os quais foram caracterizados quanto à produção de lipase, lecitinas e e protease. Para efetuar o teste de resistência a antibióticos, 21 isolados foram selecionados. Contagens entre < 1 e 108 UFC/cm2 foram observadas, com prevalência de Serratia sp., Klebsiela sp. e espécies do gênero Pseudomonas sp. Os isolados identificados apresentaram elevada atividade proteolítica e lipolítica, e a atividade de lecitinas e foi menos pronunciada. A multidroga resistência foi detectada em 54 por cento dos isolados analisados.
Descritores: Antibacterianos
Bactérias/isolamento & purificação
Leite
Lipase
Resistência Microbiana a Medicamentos
Responsável: BR91.2 - Centro de Documentação


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Texto completo SciELO Brasil
Texto completo
Id: biblio-886808
Autor: SIMÃO, ANDERSON A; MARQUES, TAMARA R; MARCUSSI, SILVANA; CORRÊA, ANGELITA D.
Título: Aqueous extract of Psidium guajava leaves: phenolic compounds and inhibitory potential on digestive enzymes
Fonte: An. acad. bras. ciênc;89(3,supl):2155-2165, 2017. tab, graf.
Idioma: en.
Resumo: ABSTRACT Leaves of Psidium guajava L. (guava) have been widely used in the popular way for prevention and treatment of various diseases. Thus, the objective of this study was to evaluate the inhibitory potential of leaves aqueous extract from three cultivars of P. guajava (Pedro Sato, Paluma and Século XXI) on α-amylase, α-glycosidase, lipase, and trypsin enzymes, in the presence or not of simulated gastric fluid and to determine the content of phenolic compounds by high performance liquid chromatography. All cultivars presented the same composition in phenolic compounds, but in different proportions. The compounds identified are gallic acid, epigallocatechin gallate, syringic acid, o-coumaric acid, resveratrol, quercetin, and catechin (which was the major compound in all the cultivars evaluated). In the absence of simulated gastric fluid, it was observed different inhibitions exercised by the leaves aqueous extracts from three cultivars of P. guajava on each enzyme. In presence of simulated gastric fluid, all cultivars showed increase in the inhibition of lipase and α-glycosidase, and decrease in inhibition of α-amylase and trypsin enzymes. These results indicate that P. guajava leaves aqueous extracts from all cultivars evaluated possess potential of use as an adjuvant in the treatment of obesity and other dyslipidemias.
Descritores: Extratos Vegetais/farmacologia
Extratos Vegetais/química
Folhas de Planta/química
Inibidores Enzimáticos/farmacologia
Antioxidantes/farmacologia
Obesidade/tratamento farmacológico
-Fenóis/análise
Água/análise
Tripsina/farmacologia
Cromatografia Líquida de Alta Pressão
Psidium/química
alfa-Amilases/farmacologia
alfa-Glucosidases/farmacologia
Lipase/farmacologia
Responsável: BR1.1 - BIREME


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Texto completo SciELO Brasil
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Id: biblio-839159
Autor: Cansian, R L; Vanin, A B; Orlando, T; Piazza, S P; Puton, B M S; Cardoso, R I; Gonçalves, I L; Honaiser, T C; Paroul, N; Oliveira, D.
Título: Toxicity of clove essential oil and its ester eugenyl acetate against Artemia salina / Toxicidade do óleo essencial de cravo e seu éster acetato de eugenila contra Artemia salina
Fonte: Braz. j. biol;77(1):155-161, Jan-Mar. 2017. tab, graf.
Idioma: en.
Resumo: Abstract The production of compounds via enzymatic esterification has great scientific and technological interest due to the several inconveniences related to acid catalysis, mainly by these systems do not fit to the concept of “green chemistry”. Besides, natural products as clove oil present compounds with excellent biological potential. Bioactives compounds are often toxic at high doses. The evaluation of lethality in a less complex animal organism can be used to a monitoring simple and rapid, helping the identification of compounds with potential insecticide activity against larvae of insect vector of diseases. In this sense, the toxicity against Artemia salina of clove essential oil and its derivative eugenyl acetate obtained by enzymatic esterification using Novozym 435 as biocatalyst was evaluated. The conversion of eugenyl acetate synthesis was 95.6%. The results about the evaluation of toxicity against the microcrustacean Artemia salina demonstrated that both oil (LC50= 0.5993 µg.mL–1) and ester (LC50= 0.1178 µg.mL–1) presented high toxic potential, being the eugenyl acetate almost 5 times more toxic than clove essential oil. The results reported here shows the potential of employing clove oil and eugenyl acetate in insecticide formulations.

Resumo A produção de compostos via esterificação enzimática possui grande interesse científico e tecnológico devido às inúmeras inconveniências relacionadas com a catálise ácida, principalmente por estes sitemas não se adequarem ao atual termo “tecnologias limpas”. Além disso, produtos naturais como o óleo de cravo, apresentam compostos com excelentes potenciais biológicos. Compostos bioativos são quase sempre tóxicos em altas doses. A avaliação da letalidade em um organismo animal menos complexo pode ser usada para um monitoramento simples e rápido, servindo também para a identificação de compostos com potencial atividade inseticida contra larvas de insetos vetores de doenças. Neste sentido, foi determinada a toxicidade frente a Artemia salina do óleo essencial de cravo e do seu derivado acetato de eugenila obtido por esterificação enzimática com lipase Novozym 435. A conversão da reação de síntese de acetato de eugenila foi de 95,6%. Os resultados referentes à avaliação da toxicidade frente ao microcrustáceo Artemia salina demonstraram que tanto o óleo (LC50= 0,5993 µg.mL–1) quanto o éster (LC50= 0,1178 µg.mL–1) apresentam elevado potencial toxicológico, sendo que o éster apresenta aproximadamente 5 vezes mais toxicidade em relação ao óleo. Estes resultados demonstram o potencial emprego do óleo de cravo e de acetato de eugenila em formulações de inseticidas.
Descritores: Artemia/efeitos dos fármacos
Óleos Voláteis/toxicidade
Óleo de Cravo/toxicidade
Inseticidas/toxicidade
-Eugenol/análogos & derivados
Eugenol/síntese química
Eugenol/toxicidade
Relação Dose-Resposta a Droga
Esterificação/efeitos dos fármacos
Larva/efeitos dos fármacos
Lipase/toxicidade
Limites: Animais
Responsável: BR1.1 - BIREME


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Id: lil-564587
Autor: Huerta, Doris; Acosta, Oscar; Miranda, Cecilia; Polo, Susan; Oré, Raquel; Bardales, Amelia; Pajuelo, Jaime.
Título: Relación del polimorfismo C-514T del gen de la lipasa hepática con indicadores nutricionales y lipoproteínas en una muestra poblacional peruana: una perspectiva nutrigenética / Relation of the hepatic lipase gene C-514T polymorphism with nutritional indicators and lipoproteins in a peruvian population sample: a nutrigenetic perspective
Fonte: An. Fac. Med. (Perú);69(4):244-249, oct.-dic. 2008. graf, tab.
Idioma: es.
Resumo: Introducción: Las enfermedades cardiovasculares son una de las principales causas de muerte en todo el mundo. El promotor del gen de la lipasa hepática presenta un polimorfismo funcional C-514T que se relaciona con la actividad de la enzima, la variación de los niveles de lipoproteínas y un posible riesgo para desarrollar enfermedades cardiovasculares. Objetivos: Establecer la relación del polimorfismo C-514T del promotor del gen de la lipasa hepática con indicadores nutricionales y los niveles de lipoproteínas plasmáticas en una muestra de peruanos saludables. Diseño: Estudio descriptivo, transversal, asociativo. Lugar: Centro de Investigación de Bioquímica y Nutrición Alberto Guzmán Barrón, Facultad de Medicina, Universidad Nacional Mayor de San Marcos. Participantes: Noventiuna personas sanas de ambos sexos, cuyas edades fluctuaban entre 18 y 58 años, voluntarios con consentimiento informado. Intervenciones: Extracción del ADN genómico a partir de muestras sanguíneas según metodología estándar. Toma de medidas antropométricas, estableciéndose los indicadores nutricionales, determinación del perfil lipídico por el método enzimático. Análisis del polimorfismo C-514T mediante la técnica de PCR/RFLP, con primers específicos y digestión con la enzima de restricción NlaIII, detectándose los fragmentos de RFLP por electroforesis en gel de poliacrilamida (PAGE) y tinción con nitrato de plata. Principales medidas de resultados: Frecuencias genotípicas y alélicas del gende la lipasa hepática y relación con parámetros lipídicos y nutricionales. Resultados: Se encontró las frecuencias genotípicas CC=0,143; CT=0,593 y TT = 0,264, siendola distribución consistente con el equilibrio de Hardy-Weinberg (X2 =3,8024, g.l.=1, p = 0,086). Las frecuencias alélicas fueron alelo C = 0,4395 y el alelo T = 0,5605. Los niveles de colesterol, HDLc, LDLc, TG y los promedios de pliegue subcutáneo, el IMC y el porcentaje de grasa en los genotipos CC, CT y TT.

Introduction: Cardiovascular diseases are major causes de death in the world. The hepatic lipase (HL) gene promoter region presents a C-514T functional polymorphism related to enzyme activity, variation of lipoproteins levels and possible cardiovascular disease risk. Objectives: To determine the association of HL gene promoter region polymorphism with both nutritional indicators and lipoproteins levels in a healthy Peruvian sample. Design: Descriptive, transversal, associative study. Setting: Alberto Guzman Barron Biochemistry and Nutrition Research Center, Faculty of Medicine, Universidad Nacional Mayor de San Marcos. Participants: Ninety healthy male and female volunteers aged 18 to 58 years. Interventions: Genomic DNA was obtained from serum samples according to standard methodology. Anthropometric measurements and lipid profile byenzymatic methods were performed. Polymorphism C-514T in the HL gene was determined by polymerase chain reaction (PCR). PCR products were digested with NlaIII and fragments separated by polyacrilamyde gel electrophoresis (PAGE) and stained with silver nitrate. Main outcome measures: HL gene genotypes and alleles frequencies and relation with both lipid and nutritional parameters. Results: We found genotype frequencies CC=0,143; CT=0,593 and TT = 0,264, consistent with Hardy-Weinberg equilibrium (X2 =3,8024, g.l. = 1, p = 0,086). Alleles frequencies were C allele = 0,4395 and T allele = 0,5605. HDLc, LDLc, TAG cholesterol levels and subcutaneous fold, BMI and fat percentage averages in CC, CT and TT genotypes did not show significant differences (p > 0,05). Nevertheless, when T allele was analyzed alone (genotypes CT and TT) according to age and sex there were significant differences (p < 0,05) in some parameters.
Descritores: Doenças Cardiovasculares
Genética
Lipase
Lipoproteínas
Polimorfismo Genético
Ranunculaceae
Limites: Humanos
Masculino
Feminino
Adulto
Pessoa de Meia-Idade
Responsável: PE1.1 - Oficina Universitária de Biblioteca


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Texto completo SciELO Brasil
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Id: lil-788974
Autor: Gururaj, P; Ramalingam, Subramanian; Devi, Ganesan Nandhini; Gautam, Pennathur.
Título: Process optimization for production and purification of a thermostable, organic solvent tolerant lipase from Acinetobacter sp. AU07
Fonte: Braz. j. microbiol;47(3):647-657, July-Sept. 2016. tab, graf.
Idioma: en.
Projeto: Department of Biotechnology, Government of India.
Resumo: ABSTRACT The purpose of this study was to isolate, purify and optimize the production conditions of an organic solvent tolerant and thermostable lipase from Acinetobacter sp. AU07 isolated from distillery waste. The lipase production was optimized by response surface methodology, and a maximum production of 14.5 U/mL was observed at 30 ºC and pH 7, using a 0.5% (v/v) inoculum, 2% (v/v) castor oil (inducer), and agitation 150 rpm. The optimized conditions from the shake flask experiments were validated in a 3 L lab scale bioreactor, and the lipase production increased to 48 U/mL. The enzyme was purified by ammonium sulfate precipitation and ion exchange chromatography and the overall yield was 36%. SDS-PAGE indicated a molecular weight of 45 kDa for the purified protein, and Matrix assisted laser desorption/ionization time of flight analysis of the purified lipase showed sequence similarity with GDSL family of lipases. The optimum temperature and pH for activity of the enzyme was found to be 50 ºC and 8.0, respectively. The lipase was completely inhibited by phenylmethylsulfonyl fluoride but minimal inhibition was observed when incubated with ethylenediaminetetraacetic acid and dithiothreitol. The enzyme was stable in the presence of non-polar hydrophobic solvents. Detergents like SDS inhibited enzyme activity; however, there was minimal loss of enzyme activity when incubated with hydrogen peroxide, Tween 80 and Triton X-100. The kinetic constants (Km and Vmax) revealed that the hydrolytic activity of the lipase was specific to moderate chain fatty acid esters. The Vmax, Km and Vmax/Km ratio of the enzyme were 16.98 U/mg, 0.51 mM, and 33.29, respectively when 4-nitrophenyl palmitate was used as a substrate.
Descritores: Compostos Orgânicos
Solventes
Proteínas de Bactérias/isolamento & purificação
Proteínas de Bactérias/biossíntese
Acinetobacter/enzimologia
Lipase/isolamento & purificação
Lipase/biossíntese
-Compostos Orgânicos/química
Solventes/química
Especificidade por Substrato
Temperatura
Proteínas de Bactérias/química
Estabilidade Enzimática
Cinética
Cromatografia por Troca Iônica
Ativação Enzimática
Espaço Extracelular/enzimologia
Concentração de Íons de Hidrogênio
Íons
Lipase/química
Lipólise
Metais
Peso Molecular
Responsável: BR1.1 - BIREME



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