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Id: lil-340766
Autor: Bonilla, César; Zavaleta, Alfonso.
Título: Estudio bioquímico del veneno de la serpiente Bothrops hyoprorus / Biochemical study of Bothrops hyoprorus`s venom
Fonte: Rev. med. exp;14(2):18-32, jul.-dic. 1997. ilus, tab.
Idioma: es.
Resumo: Se describe la purificación parcial de la enzima Fosfolipasa A del veneno de Bothrops hyoprorus y la caracterización de sus actividades hemolítica y citotóxica. La actividad fosfolipásica se detectó en dos fracciones de 23 y 12 Kd por cromatografía en Sephadex G-100 y G-50. Las actividades fosfolipásica y hemolítica fueron detectadas en las mismas fracciones. Los ensayos de Westernblot, sugieren que la fracción con 12 Kd es un producto de degradación, in vitro, del polipéptido mayor. La actividad citotóxica de la fracción fue evidente sólo a elevadas concentraciones, sugiriéndose que moléculas diferentes a Fosfolipasa A serían responsables del efecto citotóxico del veneno
Descritores: Fosfolipases
Venenos de Serpentes
Hemólise
Responsável: PE14.1 - Biblioteca de la Sede Central


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Balducci, Ivan
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Id: lil-620818
Autor: Back-Brito, Graziella Nuernberg; El Achkar, Vivian Narana Ribeiro; Garbim, Aline Lopes; Romeiro, Rogério de Lima; Jorge, Antonio Olavo Cardoso; Balducci, Ivan; Koga-ito, Cristiane Yumi.
Título: HAART therapy does not reduce the proteinase and phospholipase secretion by oral Candida albicans isolated from HIV-positive patients
Fonte: Rev. Inst. Adolfo Lutz;70(2):101-105, abr.-jun. 2011. tab, graf.
Idioma: en.
Resumo: Production of exoenzymes, specifically the proteinase and phospholipase, is considered one of the most important of pathogenicity mechanisms of C. albicans, which is crucial for tissue invasion. This study aimed at evaluating the production of these exoenzymes in 50 oral C. albicans isolates from HIV-positive (HIV+) patients treated with highly active anti-retroviral therapy (HAART), and from 50 control individuals. For testing the production of phospholipase and proteinase, the culture media containing egg yolk and bovine albumin were used, respectively. The results were obtained by measuring the diameter of the colony and divided by the diameter of colony plus the precipitation zone, defined as Pz. Data were statistically analyzed by Student's t test (5). Statistically significant difference (p = 0.001) was observed between the mean values of Pz for proteinase in isolates from HIV+ patients (Pz = 0.358±0.295) and from control group (Pz = 0.660±0.370). The same results were observed for phospholipase production (Pz = 0.399±0.227 for HIV+ group; Pz =0.635±0.292 control group). Both enzymes were highly produced by C. albicans isolated from HIV+ patients when compared with those secreted by C. albicans obtained from control group, suggesting that HAART did not reduce the secretion of these enzymes by this pathogenic fungus infecting HIV+ patients.
Descritores: HIV
Terapia Antirretroviral de Alta Atividade
Boca
Candida albicans
Fosfolipases
Peptídeo Hidrolases
Limites: Humanos
Masculino
Feminino
Tipo de Publ: GOVERNMENT PUBLICATIONS
Responsável: BR91.2 - Centro de Documentação


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Texto completo SciELO Brasil
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Id: lil-614891
Autor: Junqueira, Juliana C; Vilela, Simone F. G; Rossoni, Rodnei D; Barbosa, Júnia O; Costa, Anna Carolina B. P; Rasteiro, Vanessa M. C; Suleiman, Jamal M. A. H; Jorge, Antonio Olavo C.
Título: Oral colonization by yeasts in HIV-positive patients in Brazil / Colonização oral por leveduras em pacientes HIV-positivos no Brasil
Fonte: Rev. Inst. Med. Trop. Säo Paulo;54(1):17-24, Jan.-Feb. 2012. tab.
Idioma: en.
Projeto: FAPESP.
Resumo: INTRODUCTION: In HIV-infected patients, colonization of the oral cavity by potential pathogenic yeast may lead to development of systemic fungemia. We evaluated the prevalence of yeast in the oral cavity of Brazilian HIV-positive patients and verified whether or not the species characterized were enzymatically active. Furthermore, the species identified were tested for their susceptibility to antifungal treatment. METHODS: Patient saliva and oropharyngeal candidiasis samples were collected from 60 seropositive HIV patients and identified by the API20C system. Enzymatic activity was evaluated by the production of proteinase and phospholipase. Susceptibility to antifungal treatments were determined using the broth microdilution method. RESULTS: the most commonly isolated species were C. albicans (51.56 percent) followed by non-albicans Candida species (43.73 percent), Trichosporon mucoides (3.12 percent) and Kodamaea ohmeri (1.56 percent). Oral colonization by association of different species was observed in 42 percent of the patients. Enzymatic activity was verified in most of species isolated, except for C. glabrata, C. lusitaniae and C. guilliermondii. Resistance to Fluconazole and Amphotericin B was observed in isolates of C. albicans, C. glabrata, C. parapsilosis, C. krusei, and K. ohmeri. CONCLUSION: HIV-positive patients are orally colonized by single or multiple species of yeast that are occasionally resistant to Fluconazole or Amphotericin B.

INTRODUÇÃO: Em pacientes infectados pelo HIV, a colonização da cavidade bucal por leveduras patogênicas pode levar ao desenvolvimento de fungemias. No presente estudo, avaliamos a prevalência de leveduras na cavidade bucal de pacientes HIV-positivos e verificamos se as espécies isoladas foram enzimaticamente ativas. Além disso, as espécies identificadas foram testadas quanto à suscetibilidade a antifúngicos. MÉTODOS: Amostras de saliva e de candidose orofaríngea foram coletadas de 60 pacientes soropositivos para HIV e identificados pelo sistema API20C. A atividade enzimática foi avaliada pela produção de proteinase e fosfolipase. A suscetibilidade a antifúngicos foi determinada utilizando o método de microdiluição em caldo. RESULTADOS: As espécies mais comumente isoladas foram C. albicans (51,56 por cento), seguido por espécies de Candida não-albicans (43,73 por cento), Trichosporon mucoides (3,12 por cento) e Kodamaea ohmeri (1,56 por cento). A colonização bucal por associação de diferentes espécies foi observada em 42 por cento dos pacientes. A atividade enzimática foi verificada na maioria das espécies isoladas, com exceção de C. glabrata, C. lusitaniae e C. guilliermondii. Resistência ao fluconazol e anfotericina B foi observada em isolados de C. albicans, C. glabrata, C. parapsilosis, C. krusei, e K. ohmeri. CONCLUSÃO: Os pacientes HIV-positivos são colonizados por espécies únicas ou múltiplas de levedura que ocasionalmente são resistentes ao fluconazol ou anfotericina B.
Descritores: Infecções Oportunistas Relacionadas com a AIDS/microbiologia
Anfotericina B/farmacologia
Antifúngicos/farmacologia
Candida/classificação
Candidíase Bucal/microbiologia
Fluconazol/farmacologia
Soropositividade para HIV/microbiologia
-Candida/efeitos dos fármacos
Candida/enzimologia
Testes de Sensibilidade Microbiana
Peptídeo Hidrolases/metabolismo
Fosfolipases/metabolismo
Limites: Adulto
Feminino
Humanos
Masculino
Pessoa de Meia-Idade
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


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Texto completo
Id: biblio-828186
Autor: Ellepola, Arjuna N. B; Samaranayake, L. P; Khan, Z. U.
Título: Extracellular phospholipase production of oral Candida albicans isolates from smokers, diabetics, asthmatics, denture wearers and healthy individuals following brief exposure to polyene, echinocandin and azole antimycotics
Fonte: Braz. j. microbiol;47(4):911-916, Oct.-Dec. 2016. tab.
Idioma: en.
Projeto: Kuwait University Research Grant.
Resumo: Abstract Objective Candida albicans is the primary causative agent of oral candidosis, and one of its key virulent attributes is considered to be its ability to produce extracellular phospholipases that facilitate cellular invasion. Oral candidosis can be treated with polyenes, and azoles, and the more recently introduced echinocandins. However, once administered, the intraoral concentration of these drugs tend to be sub-therapeutic and rather transient due to factors such as the diluent effect of saliva and cleansing effect of the oral musculature. Hence, intra-orally, the pathogenic yeasts may undergo a brief exposure to antifungal drugs. We, therefore, evaluated the phospholipase production of oral C. albicans isolates following brief exposure to sub-therapeutic concentrations of the foregoing antifungals. Materials and methods Fifty C. albicans oral isolates obtained from smokers, diabetics, asthmatics using steroid inhalers, partial denture wearers and healthy individuals were exposed to sub-therapeutic concentrations of nystatin, amphotericin B, caspofungin, ketoconazole and fluconazole for one hour. Thereafter the drugs were removed and the phospholipase production was determined by a plate assay using an egg yolk-agar medium. Results The phospholipase production of these isolates was significantly suppressed with a percentage reduction of 10.65, 12.14, 11.45 and 6.40% following exposure to nystatin, amphotericin B, caspofungin and ketoconazole, respectively. This suppression was not significant following exposure to fluconazole. Conclusions Despite the sub-therapeutic, intra oral, bioavailability of polyenes, echinocandins and ketoconazole, they are likely to produce a persistent antifungal effect by suppressing phospholipase production, which is a key virulent attribute of this common pathogenic yeast.
Descritores: Fosfolipases/biossíntese
Candida albicans/efeitos dos fármacos
Candida albicans/metabolismo
Candidíase Bucal/microbiologia
Candidíase Bucal/tratamento farmacológico
Antifúngicos/farmacologia
-Polienos/uso terapêutico
Polienos/farmacologia
Azóis/uso terapêutico
Azóis/farmacologia
Candida albicans/isolamento & purificação
Candida albicans/patogenicidade
Fumar
Testes de Sensibilidade Microbiana
Dentaduras
Fatores de Virulência
Diabetes Mellitus
Ativação Enzimática
Espaço Extracelular
Equinocandinas/farmacologia
Antifúngicos/uso terapêutico
Limites: Humanos
Responsável: BR1.1 - BIREME


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Marcon, Sônia Silva
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Id: lil-732823
Autor: Rissardo, Leidyani Karina; Alvim, Neide Aparecida Titonelli; Marcon, Sonia Silva; Carreira, Lígia.
Título: Práticas de cuidado ao idoso indígena - atuação dos profissionais de saúde / The elderly care practices of indigenous-performance of health / Las prácticas de cuidado de ancianos indígenas de rendimiento de la salud
Fonte: Rev. bras. enferm;67(6):920-927, Nov-Dec/2014.
Idioma: pt.
Projeto: Fundação Araucária.
Resumo: Este estudo objetivou compreender as práticas de cuidado dos profissionais de saúde que assistem os idosos Kaingang. Estudo qualitativo, apoiado na etnografia, realizado com dez profissionais à que atuam na atenção primária saúde da Terra Indígena Faxinal, Paraná, Brasil. Os dados foram coletados no período de novembro de 2010 a fevereiro de 2012 por meio da observação participante e entrevistas, e, analisados à luz da Teoria Transcultural do Cuidado. Identificaram-se como práticas de cuidado a medicação e imunização, bem como, cuidados da medicina tradicional. Para realização destes cuidados, os profissionais dispunham de estratégias que proporcionavam manutenção dos idosos na assistência. Conclui-se que valores culturais e científicos necessitam integrar a assistência para melhoria da saúde dos idosos indígenas.

This research aims to understand the care practices of health professionals who assist the elderly Kaingang. It is a qualitative study, supported in ethnography, conducted by ten professionals working in primary health care in the indigenous land of Faxinal, Paraná, Brazil. The data was collected from November 2010 to February 2012 by participant observation and interviews, and analyzed based on the Transcultural Care Theory. Was identified the preoccupation of the carers practices with the medication and immunization, as well as traditional medical care. To achieve these, care professionals had strategies that implemented maintenance of older people in care. We conclude that cultural values and integrate scientific need assistance to improve the health of elderly indigenous.

Este estudio tuvo como objetivo entender las prácticas de cuidado de los profesionales de la salud que asisten a los ancianos Kaingang. Estudio cualitativo, apoyado en la etnografía, llevado a cabo con diez profesionales que trabajan en la atención primaria de la salud de la tierra indígena de Faxinal, Paraná, Brasil. Los datos fueron recogidos a partir de noviembre 2010 a febrero 2012 a través de la observación participante y las entrevistas, y analizado con base en la Teoría del Cuidado Transcultural. Se identificaron las prácticas de atención médica y imunizacion,el cuidado de la medicina, así tradicional. Para lograrlo, los profesionales tenían estrategias que proporcionaban el mantenimiento de las personas mayores en su atención. Se concluye que los valores culturales y científicos necesitan ayuda para mejorar la salud de los ancianos indígenas.
Descritores: Fígado/enzimologia
Lisossomos/enzimologia
Fosfolipases A/metabolismo
Fosfolipases/metabolismo
Inibidores de Proteases/farmacologia
-Células Cultivadas
Quimotripsina/antagonistas & inibidores
Inibidores de Cisteína Proteinase/farmacologia
Leucina/análogos & derivados
Leucina/farmacologia
Leupeptinas/farmacologia
Oligopeptídeos/farmacologia
Pepstatinas/farmacologia
Fosfolipases A1
Fatores de Tempo
Limites: Animais
Ratos
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


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Id: biblio-893617
Autor: GOMES, Cinthya Cristina; GUIMARÃES, Ludmila Silva; PINTO, Larissa Christina Costa; CAMARGO, Gabriela Alessandra da Cruz Galhardo; VALENTE, Maria Isabel Bastos; SARQUIS, Maria Inêz de Moura.
Título: Investigations of the prevalence and virulence of Candida albicans in periodontal and endodontic lesions in diabetic and normoglycemic patients
Fonte: J. appl. oral sci;25(3):274-281, May-June 2017. tab, graf.
Idioma: en.
Projeto: Rio de Janeiro Research Foundation.
Resumo: Abstract Pulpal and periodontal tissues have similar microbiota that allows cross-contamination between the pulp and periodontal tissues. Objective The aim of this study was to investigate the prevalence of isolated Candida albicans from periodontal endodontic lesions in diabetic and normoglycemic patients, and the fungi's virulence in different atmospheric conditions. Material and Methods A case-control study was conducted on 15 patients with type 2 diabetes mellitus (G1) and 15 non-diabetics (G2) with periodontal endodontic lesions. Samples of root canals and periodontal pockets were plated on CHROMagar for later identification by polymerase chain reaction (PCR) and virulence test. Results C. albicans was identified in 79.2% and 20.8% of the 60 samples collected from diabetic and normoglycemic patients, respectively. Of the 30 samples collected from periodontal pockets, 13 showed a positive culture for C. albicans, with 77% belonging to G1 and 23% to G2. Of the 11 positive samples from root canals, 82% were from G1 and 18% from G2. Production of proteinase presented a precipitation zone Pz<0.63 of 100% in G1 and 72% in G2, in redox and negative (Pz=1), under anaerobic conditions in both groups. Hydrophobicity of the strains from G1 indicated 16.4% with low, 19.3% with moderate, and 64.3% with high hydrophobicity in redox. In G2, 42.2% had low, 39.8% had moderate, 18% had high hydrophobicity in redox. In anaerobic conditions, G1 showed 15.2% with low, 12.8% with moderate, and 72% with high hydrophobicity; in G2, 33.6% had low, 28.8% had moderate, and 37.6% had high hydrophobicity. There was statistical difference in the number of positive cultures between G1 and G2 (p<0.05) with predominance in G1. There was statistical difference for all virulence factors, except hemolysis (p=0.001). Conclusions Candida albicans was isolated more frequently and had higher virulence in diabetic patients.
Descritores: Doenças Periodontais/microbiologia
Candida albicans/isolamento & purificação
Candida albicans/patogenicidade
Doenças da Polpa Dentária/microbiologia
Diabetes Mellitus Tipo 2/microbiologia
-Oxirredução
Peptídeo Hidrolases/análise
Doenças Periodontais/fisiopatologia
Doenças Periodontais/diagnóstico por imagem
Bolsa Periodontal/microbiologia
Fosfolipases/análise
Virulência
DNA Fúngico
Radiografia Dentária
Estudos de Casos e Controles
Reação em Cadeia da Polimerase
Estatísticas não Paramétricas
Cavidade Pulpar/microbiologia
Doenças da Polpa Dentária/fisiopatologia
Doenças da Polpa Dentária/diagnóstico por imagem
Diabetes Mellitus Tipo 2/fisiopatologia
Eletroforese
Interações Hidrofóbicas e Hidrofílicas
Limites: Humanos
Masculino
Feminino
Adulto
Pessoa de Meia-Idade
Idoso
Responsável: BR1.1 - BIREME


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Id: biblio-868801
Autor: Toro Zúñiga, Viviana; Brevis Azocar, Pedro.
Título: Aislamiento presuntivo y caracterización de cryptococcus neoformans y cryptococcus gattii desde árboles en la región de O'Higgins y Maule, Chile / Presumptive isolation and characterization of cryptococcus neoformans and cryptococcus gattii from trees in the region of O'Higgins and Maule, Chile
Fonte: Bol. micol. (Valparaiso En linea);30(2):6-15, dic. 2015. ilus, tab.
Idioma: es.
Resumo: Introducción: la criptocococis es una micosis sistémica causada por C. neoformans y C. gattii, es frecuente y oportunista en inmunocomprometidos y patógeno primario en personas inmunocompetentes. C. neoformans tiene una distribución mundial y se ha aislado desde las excretas de palomas. C. gattii se considera restringida a regiones con clima tropical, subtropical, y templadas, se encuentra asociada frecuentemente a detritos de especies de Eucalyptus sp. La virulencia de estas levaduras le permite desarrollar patogénesis en mamíferos y supervivencia en el ambiente. Objetivo: Identificar y determinar la actividad de proteinasas y fosfolipasas, de C. neoformans y C. gattii aisladas desde las oquedades de árboles en lugares con alta afluencia de público. Materiales y Métodos: Se tomaron 200 muestras de hisopado desde distintas especies de árboles desde sectores de la región de O'Higgins y el Maule. Se siembran en ASG, se aíslan y mantienen en ASD. Identificación con tinta china, Urea de Christensen, crecimiento a 37°C, asimilación y fermentación de azucares, y siembra en medio CGB. Se mide índice de actividad enzimática Prz de proteinasas y fofolipasas. Resultados y Conclusiones: Se obtuvieron 109 cepas de C. neoformans aisladas desde las oquedades de diferentes especies arbóreas y 3 cepas presuntivas de C. gattii desde Eucalyptus sp. y Prunus cerasifera artropurpurea. El 88,1 por ciento de las cepas C. neoformans y 100 por ciento de C. gattii, presentaron alta actividad proteolítica, El 49,5 por ciento de las cepas de C. neoformans y 33,3 por ciento de C. gattii mostraron alta actividad de fosfolipasas.

Introduction: criptocococis is a systemic mycosis caused by C. neoformans and C. gattii, frequent and opportunistic in immunocompromised and primary pathogen in immunocompetent persons. C. neoformans has a worldwide distribution and has been isolated from the excreta of pigeons. C. gattii is considered restricted to regions with tropical, subtropical, and temperate, is often associated with species of Eucalyptus sp. The virulence of these yeasts develop pathogenesis allows survival in mammals and the environment. Objective: To identify and determine the activity of proteinases and phospholipases of C. neoformans and C. gattii isolated from the hollows of trees in places with high turnout. Materials and Methods: 200 swab samples were taken from different species of trees from areas of the region of O'Higgins and Maule. Planted in ASG, they are isolated and kept in ASD. Identification with ink, Urea Christensen, growth at 37 ° C, assimilation and fermentation of sugars, and planting medium CGB. Prz index proteinase enzyme activity is measured and phospholipases. Results and Conclusions: We manage to get 109 strains of C. neoformans isolated from the hollows of different tree species and 3 presumptive strains of C. gattii from Eucalyptus sp. and Prunus cerasifera artropurpurea. 88.1 percent of the strains C. neoformans and C. gattii 100 percent , they showed high proteolytic activity, 49.5 percent of the strains of C. neoformans and C. gattii 33.3 percent showed high activity phospholipases.
Descritores: Cryptococcus gattii/isolamento & purificação
Cryptococcus gattii/enzimologia
Cryptococcus gattii/patogenicidade
Cryptococcus neoformans/isolamento & purificação
Cryptococcus neoformans/enzimologia
Cryptococcus neoformans/patogenicidade
Peptídeo Hidrolases
Fosfolipases
-Árvores/microbiologia
Chile
Criptococose/etiologia
Eucalyptus/microbiologia
Pneumopatias Fúngicas
Prunus/microbiologia
Limites: Humanos
Responsável: CL2.1 - Biblioteca de Medicina


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Id: lil-701306
Autor: Rossoni, Rodnei Dennis; Barbosa, Júnia Oliveira; Vilela, Simone Furgeri Godinho; Santos, Jéssica Diane dos; Jorge, Antonio Olavo Cardoso; Junqueira, Juliana Campos.
Título: Correlation of phospholipase and proteinase production of Candida with in vivo pathogenicity in Galleria mellonella
Fonte: Braz. j. oral sci;12(3):199-204, July-Sept. 2013. graf, tab.
Idioma: en.
Projeto: The São Paulo State Research Foundation - FAPESP.
Resumo: An essential factor to the virulence of the genus Candida is the ability to produce enzymes and this may be crucial in the establishment of fungal infections. AIM:This study investigated in vitro enzymatic activities of Candida species and their virulence in an in vivo Galleria mellonella experimental model. METHODS: Twenty-four clinical strains of Candida spp. isolated from the human oral cavity were evaluated, including the following species: C. albicans, C. dubliniensis, C. glabrata, C. tropicalis, C. krusei, C. parapsilosis, C. norvegensis, C. lusitaniae and C. guilliermondii. All Candida strains were tested in vitro for production of proteinase and phospholipase. The Candida strains were also injected into Galleria mellonella larvae to induce experimental candidiasis, and after 24 hours, the survival rate was assessed. RESULTS: Phospholipase and proteinase activity were observed in 100% of the C. albicans strains. In the non-albicans species, proteinase and phospholipase activity were observed in 25 and 43% of the studied strains, respectively. The most pathogenic Candida species in G. mellonella were C. albicans, C. dubliniensis and C. lusitaniae, whereas C. glabrata was the least virulent species. Furthermore, a positive significant correlation was found between both enzymatic activities with virulence in G. mellonella. CONCLUSIONS: The virulence of Candida strains in G. mellonella is related to the quantity of proteinases and phospholipases production of each strain.
Descritores: Candida/patogenicidade
Invertebrados/patogenicidade
Peptídeo Hidrolases
Fosfolipases
Fatores de Virulência
Limites: Humanos
Responsável: BR218.1 - Biblioteca Carlos Henrique Robertson Liberalli


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Id: lil-686621
Autor: Rodriguez-Ravelo, Rodolfo; Coronas, Fredy I V; Zamudio, Fernando Z; Gonzalez-Morales, Lidia; Lopez, Georgina Espinosa; Urquiola, Ariel Ruiz; Possani, Lourival D.
Título: The Cuban scorpion Rhopalurus junceus (Scorpiones, Buthidae): component variations in venom samples collected in different geographical areas
Fonte: J. venom. anim. toxins incl. trop. dis;19:13-13, maio 2013.
Idioma: en.
Resumo: Backgound: The venom of the Cuban scorpion Rhopalurus junceus is poorly study from the point of view of their components at molecular level and the functions associated. The purpose of this article was to conduct a proteomic analysis of venom components from scorpions collected in different geographical areas of the country. Results: Venom from the blue scorpion, as it is called, was collected separately from specimens of five distinct Cuban towns (Moa, La Poa, Limonar, El Chote and Farallones) of the Nipe-Sagua-Baracoa mountain massif and fractionated by high performance liquid chromatography (HPLC); the molecular masses of each fraction were ascertained by mass spectrometry analysis. At least 153 different molecular mass components were identified among the five samples analyzed. Molecular masses varied from 466 to 19755 Da. Scorpion HPLC profiles differed among these different geographical locations and the predominant molecular masses of their components. The most evident differences are in the relative concentration of the venom components. The most abundant components presented molecular weights around 4 kDa, known to be K+-channel specific peptides, and 7 kDa, known to be Na+-channel specific peptides, but with small molecular weight differences. Approximately 30 peptides found in venom samples from the different geographical areas are identical, supporting the idea that they all probably belong to the same species, with some interpopulational variations. Differences were also found in the presence of phospholipase, found in venoms from the Poa area (molecular weights on the order of 14 to 19 kDa). The only ubiquitous enzyme identified in the venoms from all five localities studied (hyaluronidase) presented the same 45 kD molecular mass, identified by gel electrophoresis analysis. Conclusions: The venom of these scorpions from different geographical areas seem to be simila, and are rich in peptides that have of the same molecular masses of the peptides...
Descritores: Peptídeos
Fosfolipases
Proteômica
Venenos de Escorpião/isolamento & purificação
-Cuba/epidemiologia
Espectrometria de Massas/métodos
Limites: Animais
Responsável: BR33.1 - Divisão Técnica de Biblioteca e Documentação


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ALVES, Sydney Hartz
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Id: lil-679510
Autor: Mattei, Antonella Souza; Alves, Sydney Hartz; Severo, Cecilia Bittencourt; Guazzelli, Luciana da Silva; Oliveira, Flavio de Mattos; Severo, Luiz Carlos.
Título: Determination of germ tube, phospholipase, and proteinase production by bloodstream isolates of Candida albicans
Fonte: Rev. Soc. Bras. Med. Trop;46(3):340-342, May-Jun/2013. tab.
Idioma: en.
Resumo: Introduction Candida albicans is a commensal and opportunistic agent that causes infection in immunocompromised individuals. Several attributes contribute to the virulence and pathogenicity of this yeast, including the production of germ tubes (GTs) and extracellular hydrolytic enzymes, particularly phospholipase and proteinase. This study aimed to investigate GT production and phospholipase and proteinase activities in bloodstream isolates of C. albicans. Methods One hundred fifty-three C. albicans isolates were obtained from blood samples and analyzed for GT, phospholipase, and proteinase production. The assays were performed in duplicate in egg yolk medium containing bovine serum albumin and human serum. Results Detectable amounts of proteinase were produced by 97% of the isolates, and 78% of the isolates produced phospholipase. GTs were produced by 95% of the isolates. A majority of the isolates exhibited low levels of phospholipase production and high levels of proteinase production. Conclusions Bloodstream isolates of C. albicans produce virulence factors such as GT and hydrolytic enzymes that enable them to cause infection under favorable conditions. .
Descritores: Candida albicans/enzimologia
Candida albicans/crescimento & desenvolvimento
Peptídeo Hidrolases/biossíntese
Fosfolipases/biossíntese
Fatores de Virulência/biossíntese
-Candida albicans/patogenicidade
Soroalbumina Bovina
Limites: Animais
Bovinos
Humanos
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME



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