Base de dados : LILACS
Pesquisa : D08.811.277.352.650.025.500 [Categoria DeCS]
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Id: biblio-893662
Autor: Öncel Torun, Zeynep; Torun, Deniz; Baykal, Barış; Öztuna, Ali; Yeşildal, Fatih; Avcu, Ferit.
Título: Effects of triethylene glycol dimethacrylate (TEGDMA) on the odontoclastic differentiation ability of human dental pulp cells
Fonte: J. appl. oral sci;25(6):631-640, Nov.-Dec. 2017. tab, graf.
Idioma: en.
Projeto: Scientific and Technological Research Council of Turkey.
Resumo: Abstract Objectives: The primary purpose of this study was to examine the effects of triethylene glycol dimethacrylate (TEGDMA) on odontoclastic differentiation in the dental pulp tissue. Material and Methods: The effects of different TEGDMA dosages on the odontoclastic differentiation capability of dental pulp cells were analyzed in vitro using the following methodologies: i) flow cytometry and tartrate-resistant acid phosphatase (TRAP) staining; ii) apoptotic effects using Annexin V staining; iii) mRNA expression of osteoprotegerin (OPG) and receptor activator of nuclear factor (NF)-kB ligand (RANKL) genes by quantitative Real-time PCR (qRT-PCR); and iv) OPG and RANKL protein expression by enzyme-linked immunosorbent assay (ELISA). Results: TEGDMA caused relatively less odontoclastic differentiation in comparison with the control group; however, odontoclastic differentiation augmented with increasing doses of TEGDMA (p<0.05). The mRNA and protein expression of OPG was lower in TEGDMA treated pulp cells than in the control group (p<0.05). While the mRNA expression of RANKL remained unchanged compared to the control group (p>0.05), its protein expression was higher than the control group (p<0.05). In addition, TEGDMA increased the apoptosis of dental pulp cells dose dependently. Conclusions: TEGDMA reduced the odontoclastic differentiation ability of human dental pulp cells. However, odontoclastic differentiation ratios increased proportionally with the increasing dose of TEGDMA.
Descritores: Diferenciação Celular/efeitos dos fármacos
Polpa Dentária/efeitos dos fármacos
Polietilenoglicóis/farmacologia
Ácidos Polimetacrílicos/farmacologia
Fosfatase Ácida Resistente a Tartarato/efeitos dos fármacos
-Polpa Dentária/citologia
Ensaio de Imunoadsorção Enzimática
Citometria de Fluxo
Receptores de Lipopolissacarídeos/metabolismo
Ligante RANK/metabolismo
Reação em Cadeia da Polimerase em Tempo Real
Limites: Seres Humanos
Responsável: BR1.1 - BIREME


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Id: biblio-880311
Autor: Tobias, Kátia Regina Coimbra.
Título: Imunomarcação para TRAP em tecido de reparação óssea de ratos espontaneamente hipertensos (SHR) tratados com Atenolol / Immunostaining for TRAP in bone healing tissue of Spontaneously Hypertensive Rats (SHR) treated with Atenolol.
Fonte: Araçatuba; s.n; 2017. 42 p. graf, ilus.
Idioma: pt.
Tese: Apresentada a Universidade Estadual Paulista "Julio de Mesquita Filho". Faculdade de Odontologia de Araçatuba para obtenção do grau de Doutor.
Resumo: Introdução: A hipertensão arterial tem sido um dos maiores problemas de saúde no mundo, com grandes alterações para as doenças cardiovasculares e renais. O tecido ósseo tem função importante no suporte, proteção e locomoção e está sob o controle de fatores sistêmicos como hormônios e fatores locais, entre eles os fatores de crescimento e citocinas. A Fosfatase Ácida Tartarato Resistente (TRAP) é uma enzima que faz parte da família das fosfatases ácidas e apresenta localização intracelular; mais especificamente dentro do compartimento lisossomal de osteoclasto, macrófagos e células dendríticas, tem sido utilizada como um marcador histoquímico da atividade osteoclástica. Objetivos: Avaliar a expressão da proteína TRAP em alvéolos dentários de ratos hipertensos (SHR) e normotensos tratados ou não com atenolol. Métodos: Neste estudo foram utilizados 4 grupos de ratos sendo: 1) W (wistar sem tratamento), 2) WT (wistar tratado com atenolol), 3) S (SHR sem tratamento) e 4) ST (SHR tratado com atenolol), submetidos a exodontia do incisivo superior direito, com eutanásia no 7º, 14º, 21 e 28º dia pós-operatório. A análise dos mecanismos biológicos envolvidos no processo de reparo alveolar foi obtida pela análise da expressão de proteínas TRAP por meio da técnica de imunoistoquímica. Os resultados foram analisados pela média e erro padrão da média e aplicado o teste paramétrico ANOVA, com pos-test de Tukey para avaliar os períodos dentro de cada grupo e entre os grupos, sendo consideradas as diferenças significativas quando p<0,05. Resultados: Os resultados mostraram que a marcação TRAP aumenta em alvéolo dentais de ratos Wistar durante todos os períodos pós ­ operatórios. A marcação TRAP aumenta apenas ao 14o nos dias de reparação alveolar em alvéolo dental de SHR não tratados. O atenolol não altera o processo de reparo alveolar em ratos Wistar, porém o atenolol promoveu a redução da marcação de TRAP em SHR ao 14º dia. Conclusão: A hipertensão aumenta a expressão da proteína TRAP no 14o dia pós-cirúrgico de reparação alveolar e o atenolol promove redução da marcação aumentada de TRAP ao 14º dia pós-cirúrgico em alvéolos de SHR(AU)

Introduction: Arterial hypertension has been one of the world's biggest health problems, with considerable alterations for cardiovascular and renal diseases. The bone tissue has an important role in support, protection and locomotion and is controlled by systemic factors like hormones and local factors, such as growth factors and cytokines. The Tartrate-resistant Acid Phosphatase (TRAP) is an enzyme that belongs to the Acid Phosphatases family and has an intracellular location, more specifically inside the lysosomal compartment of osteoclasts, macrophages and dendritic cells. It has been used as a histochemical marker of the osteoclast activity. Objectives: Evaluate TRAP protein's expression in the dental alveoli of normotensive and hypertensive rats (SHR) treated or not treated with Atenolol. Methods: In this study, four groups of rats were used: 1) W (with no treatment), 2) WT (wistar treated with Atenolol), 3) S (SHR without treatment) and 4) ST (SHR treated with Atenolol), all of which underwent exodontia of the upper right incisor with euthanasia on the 7th, 14th, 21st and 28th day after the operation. The analysis of the biological mechanisms involved in the process of alveolar repair was obtained by the expression of TRAP proteins in the alveolar process through an immunohistochemistry technique. The results were analyzed through the average and its standard error. The parametric test ANOVA was applied with Tukey's posttest were applied to evaluate the periods within each group and between the groups, considering the significant differences when p< 0,05. Results: The results demonstrated that TRAP staining increases in the dental alveoli of Wistar rats during all the post-surgical periods. TRAP staining increases only on the 14th day of alveolar recovery in the dental alveoli of non-treated SHR. Atenolol does not change the process of alveolar repair in Wistar rats, but Atenolol promoted the reduction of TRAP staining among SHR on the 14th day. Conclusion: Hypertension increases the expression of TRAP proteins on the 14th alveolar recovery postsurgical day and Atenolol promotes the reduction of the increased TRAP staining on the 14th postsurgical day in SHR's alveoli(AU)
Descritores: Atenolol
Hipertensão
Cirurgia Bucal
Fosfatase Ácida Resistente a Tartarato
-Imuno-Histoquímica
Ratos Endogâmicos SHR
Alvéolo Dental
Limites: Animais
Ratos
Responsável: BR186.1 - Biblioteca Honório Monteiro
BR186.1


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Volpon, José B
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Id: biblio-840064
Autor: Spirlandeli, Adriano L; Dick-de-Paula, Ingrid; Zamarioli, Ariane; Jorgetti, Vanda; Ramalho, Leandra NZ; Nogueira-Barbosa, Marcello H; Volpon, Jose B; Jordão, Alceu A; Cunha, Fernando Q; Fukada, Sandra Y; de Paula, Francisco JA.
Título: Hepatic Osteodystrophy: The Mechanism of Bone Loss in Hepatocellular Disease and the Effects of Pamidronate Treatment
Fonte: Clinics;72(4):231-237, Apr. 2017. tab, graf.
Idioma: en.
Projeto: CNPq; . NAP-DIN; . CNPq.
Resumo: OBJECTIVES: The present study was designed to evaluate the bone phenotypes and mechanisms involved in bone disorders associated with hepatic osteodystrophy. Hepatocellular disease was induced by carbon tetrachloride (CCl4). In addition, the effects of disodium pamidronate on bone tissue were evaluated. METHODS: The study included 4 groups of 15 mice: a) C = mice subjected to vehicle injections; b) C+P = mice subjected to vehicle and pamidronate injections; c) CCl4+V = mice subjected to CCl4 and vehicle injections; and d) CCl4+P = mice subjected to CCl4 and pamidronate injections. CCl4 or vehicle was administered for 8 weeks, while pamidronate or vehicle was injected at the end of the fourth week. Bone histomorphometry and biomechanical analysis were performed in tibiae, while femora were used for micro-computed tomography and gene expression. RESULTS: CCl4 mice exhibited decreased bone volume/trabecular volume and trabecular numbers, as well as increased trabecular separation, as determined by bone histomorphometry and micro-computed tomography, but these changes were not detected in the group treated with pamidronate. CCl4 mice showed increased numbers of osteoclasts and resorption surface. High serum levels of receptor activator of nuclear factor-κB ligand and the increased expression of tartrate-resistant acid phosphatase in the bones of CCl4 mice supported the enhancement of bone resorption in these mice. CONCLUSION: Taken together, these results suggest that bone resorption is the main mechanism of bone loss in chronic hepatocellular disease in mice.
Descritores: Conservadores da Densidade Óssea/farmacologia
Doenças Ósseas Metabólicas/tratamento farmacológico
Doenças Ósseas Metabólicas/etiologia
Remodelação Óssea/efeitos dos fármacos
Difosfonatos/farmacologia
Hepatopatias/complicações
-Osso e Ossos/diagnóstico por imagem
Osso e Ossos/efeitos dos fármacos
Osso e Ossos/metabolismo
Doenças Ósseas Metabólicas/metabolismo
Reabsorção Óssea/metabolismo
Tetracloreto de Carbono
Subunidade alfa 1 de Fator de Ligação ao Core/genética
Modelos Animais de Doenças
Cirrose Hepática/induzido quimicamente
Cirrose Hepática/metabolismo
Hepatopatias/metabolismo
Camundongos Endogâmicos C57BL
Osteoprotegerina/genética
Fósforo/administração & dosagem
Ligante RANK/genética
Fosfatase Ácida Resistente a Tartarato/genética
Microtomografia por Raio-X
Limites: Animais
Masculino
Responsável: BR1.1 - BIREME


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Marcantonio, Rosemary Adriana Chiérice
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Id: biblio-839509
Autor: OLIVEIRA, Guilherme José Pimentel Lopes de; PAULA, Luiz Guilherme Freitas de; SOUZA, João Antônio Chaves de; SPIN-NETO, Rubens; STAVROPOULOS, Andreas; MARCANTONIO, Rosemary Adriana Chiérici.
Título: Effects of avocado/soybean unsaponifiables (ASU) on the treatment of ligature-induced periodontitis in rats
Fonte: Braz. oral res. (Online);31:e28, 2017. tab, graf.
Idioma: en.
Projeto: Fundação de amparo a pesquisa do estado de São Paulo; . Fundação de amparo a pesquisa do estado de São Paulo.
Resumo: Abstract The purpose of this study is to evaluate the effect of the avocado/soybean unsaponifiables (ASU) on the treatment of induced periodontitis in rats. Periodontitis was induced in 84 rats via ligature placement around the second upper molar, which was removed after 7 days, and scaling and root planning (SRP) was performed at this time. Subsequently, the rats were randomly allocated to four groups with 21 animals each: One SRP group in which saline solution was administered (SS), and three groups in which ASU was administered (0.6 g/kg/day), beginning either 7 days before the induction of periodontitis (SRP/ASU-7), on the day of periodontitis induction (SRP/ASU0), or on the day of treatment (SRP/ASU+7). ASU and SS were administered daily by gavage until the sacrifice of the animals (7, 15, and 30 days after SRP). The % bone in the furcation area was evaluated by histomorphometry and micro-CT. The expression of proteins (TRAP, RANKL, and alkaline phosphatase) and mRNA (IL-1β, TNF-α, IL-6, RANKL, and alkaline phosphatase) were evaluated by immunohistochemistry and qPCR. The SRP/ASU+7 group presented a higher percentage of bone fill in the furcation area and higher expression of alkaline phosphatase than in the SRP group (at 7 and 30 days, respectively). The SRP/ASU0 and SRP/ASU+7 groups presented lower expression levels of RANKL mRNA than the SRP and SRP/ASU-7 groups at 15 days. ASU administration on the day of the SRP treatment of the ligature-induced periodontitis promoted subtle beneficial effects on periodontal repair following the treatment of induced periodontitis within the experimental period of 7 days.
Descritores: Periodontite/tratamento farmacológico
Persea/química
Extratos Vegetais/uso terapêutico
Feijão de Soja/química
-Expressão Gênica
Imuno-Histoquímica
Interleucina-1beta/análise
Interleucina-6/análise
Periodontite/etiologia
Periodontite/patologia
Distribuição Aleatória
Ligante RANK/análise
Ratos Sprague-Dawley
Reação em Cadeia da Polimerase em Tempo Real
Valores de Referência
Reprodutibilidade dos Testes
Aplainamento Radicular/métodos
Fosfatase Ácida Resistente a Tartarato/análise
Fatores de Tempo
Resultado do Tratamento
Fator de Necrose Tumoral alfa/análise
Limites: Animais
Masculino
Tipo de Publ: Estudos de Avaliação
Responsável: BR1.1 - BIREME



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