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ARANA-CHAVEZ, Victor Elias
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Id: biblio-1011567
Autor: Friedrichsdorf, Simone Peixe; Arana-Chavez, Victor Elias; Bradaschia-Correa, Vivian; Cattaneo, Paolo Maria; Dominguez, Gladys Cristina.
Título: Infrared light-emitting diode (led) effects on orthodontic tooth movement
Fonte: Braz. dent. j;30(4):410-416, July-Aug. 2019. tab, graf.
Idioma: en.
Resumo: Abstract The present study aimed to analyze the effect of LED phototherapy on the presence of hyalinization and root resorption during orthodontic tooth movement (OTM) in rats and to measure the amount of tooth movement. Eighty rats were allocated into two groups: LED and control (CON), where the LED rats were irradiated with infrared LED (850 nm, 30 mW) for 5 min during the first five days of OTM and where controls were not irradiated. Both groups were subdivided into four subgroups (n=10) according to the date of euthanasia (4, 7, 14 and 21 days). Five out of ten LED21 and five of ten CON21 rats were submitted to micro-computed tomography (μCT); μCT scans were taken on days 0, 7, 14 and 21. For histological study, maxillae were processed to light microscopy using Hematoxylin-Eosin (HE) and Tartrate-Resistant Acid Phosphatase (TRAP) histochemistry. The amount of tooth movement did not differ between LED and CON. Hyalinization was observed at the pressure areas in both groups, and it did not show a statistically significant difference between the groups. Root resorption was also observed in both groups after 7 days and it did not represent any differences between the two groups. LED phototherapy was not able to increase the amount of OTM. Similar characteristics of hyalinization and root resorption were observed in both groups.

Resumo O presente estudo tem como objetivo analisar o efeito da fototerapia LED na presença da hialinização e reabsorção radicular durante o movimento dentário ortodôntico (MDO) em ratos, e a mensuração da quantidade de movimento dentário. Oitenta ratos foram alocados em dois grupos: LED e Controle (CON), os ratos foram irradiados com um LED infravermelho (850nm, 30mW) por 5 minutos durante os cinco primeiros dias da MDO; e o grupo controle não foi irradiado. Ambos os grupos foram subdivididos em 4 subgrupos (n=10) de acordo com a data da eutanásia (4, 7, 14 e 21 dias). Cinco dos dez ratos LED21 e cinco dos dez ratos CON21foram submetidos a microtomografia computadorizada (μCT); As μCT foram realizadas nos dias 0, 7, 14 e 21. Para o estudo histológico, as maxilas foram processadas para microscopia de luz, usando hematoxilina-eosina (HE) e Fosfatase ácido Tartrate-Resistente (TRAP) para histoquímica. A quantidade de movimento dentário não diferiu entre o LED e o CON. A hialinização foi observada nas áreas de pressão em ambos os grupos e não mostrou diferença estatisticamente significante. Reabsorção radicular também foi observada em ambos os grupos depois de 7 dias e não houve diferença entre os grupos. A fototerapia LED não aumentou a quantidade de MDO. Características similares de hialinização e reabsorção radicular foram observadas em ambos os grupos
Descritores: Reabsorção da Raiz
Técnicas de Movimentação Dentária
-Ratos Wistar
Microtomografia por Raio-X
Fosfatase Ácida Resistente a Tartarato
Limites: Animais
Ratos
Responsável: BR1.1 - BIREME


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Id: biblio-954523
Autor: Hassumi, Jaqueline Suemi; Mulinari-Santos, Gabriel; Fabris, André Luis da Silva; Jacob, Ricardo Garcia Mureb; Gonçalves, Alaíde; Rossi, Ana Cláudia; Freire, Alexandre Rodrigues; Faverani, Leonardo Pérez; Okamoto, Roberta.
Título: Alveolar bone healing in rats: micro-CT, immunohistochemical and molecular analysis
Fonte: J. appl. oral sci;26:e20170326, 2018. graf.
Idioma: en.
Projeto: UNESP; . São Paulo Research Foundation.
Resumo: Abstract Alveolar bone healing after upper incisor extraction in rats is a classical model of preclinical studies. The underlying morphometric, cellular and molecular mechanism, however, remains imprecise in a unique study. Objectives The aim of this study was therefore to characterize the alveolar bone healing after upper incisor extraction in rats by micro computed tomographic (Micro-CT), immunohistochemical and real-time polymerase chain reaction (RT-PCR) analysis. Material and Methods Thirty animals (Rattus norvegicus, Albinus Wistar) were divided into three groups after upper incisors extraction at 7, 14, and 28 days. Micro-CT was evaluated based on the morphometric parameters. Subsequently, the histological analyses and immunostaining of osteoprotegerin (OPG), receptor activator of nuclear kappa B ligand (RANKL) and tartrate resistant acid phosphate (TRAP) was performed. In addition, RT-PCR analyses of OPG, RANKL, the runt-related transcription factor 2 (RUNX2), osteocalcin (OC), osteopontin (OPN), osterix (OST) and receptor activator of nuclear kappa B (RANK) were performed to determine the expression of these proteins in the alveolar bone healing. Results Micro-CT: The morphometric parameters of bone volume and trabecular thickness progressively increased over time. Consequently, a gradual decrease in trabecular separation, trabecular space and total bone porosity was observed. Immunohistochemical: There were no differences statistically significant between the positive labeling for OPG, RANKL and TRAP in the different periods. RT-PCR: At 28 days, there was a significant increase in OPG expression, while RANKL expression and the RANKL/OPG ratio both decreased over time. Conclusion Micro-CT showed the newly formed bone had favorable morphometric characteristics of quality and quantity. Beyond the RUNX2, OC, OPN, OST, and RANK proteins expressed in the alveolar bone healing, OPG and RANKL activity showed to be essential for activation of basic multicellular units during the alveolar bone healing.
Descritores: Cicatrização/fisiologia
Remodelação Óssea/fisiologia
Alvéolo Dental/fisiologia
Alvéolo Dental/diagnóstico por imagem
-Valores de Referência
Fatores de Tempo
Extração Dentária
Fatores de Transcrição/análise
Imuno-Histoquímica
Expressão Gênica
Osteocalcina/análise
Reprodutibilidade dos Testes
Ratos Wistar
Reação em Cadeia da Polimerase Via Transcriptase Reversa
Subunidade alfa 1 de Fator de Ligação ao Core/análise
Osteopontina/análise
Ligante RANK/análise
Osteoprotegerina/análise
Microtomografia por Raio-X
Fosfatase Ácida Resistente a Tartarato/análise
Limites: Humanos
Masculino
Tipo de Publ: Estudo de Avaliação
Responsável: BR1.1 - BIREME


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Id: biblio-893737
Autor: Fabris, André Luis da Silva; Faverani, Leonardo Perez; Gomes-Ferreira, Pedro Henrique Silva; Polo, Tárik Ocon Braga; Santiago-Júnior, Joel Ferreira; Okamoto, Roberta.
Título: Bone repair access of BoneCeramic™ in 5-mm defects: study on rat calvaria
Fonte: J. appl. oral sci;26:e20160531, 2018. graf.
Idioma: en.
Projeto: FAPESP - São Paulo Research Foundation.
Resumo: Abstract Objective: The aim of this study was to evaluate the osteoconductive potential of BoneCeramic™ on bone healing in rat calvaria 5-mm defects. Material and Methods: A 5-mm calvaria bone defect was induced in three groups and the defect was not filled with biomaterial [Clot Group (CG)], autogenous bone (AG), or Bone Ceramic Group (BCG). Animals were euthanized after 14 or 28 days and the bone tissue within the central area of the bone defect was evaluated. Results were compared using ANOVA and Tukey test (p<0.05). Immunohistochemistry was performed using primary antibodies against osteocalcin, RUNX-2, TRAP, VEGF proteins, and 3-dimensional images of the defects in μCT were obtained to calculate bone mineral density (BMD). Results: In BCG, the defect was completely filled with biomaterial and new bone formation, which was statistically superior to that in the GC group, at both time-points (p<0.001 for 14 days; p=0.002 for 28 days). TRAP protein showed weak, RUNX-2 showed a greater immunolabeling when compared with other groups, VEGF showed moderate immunostaining, while osteocalcin was present at all time-points analyzed. The μCT images showed filling defect by BCG (BMD= 1337 HU at 28 days). Conclusion: Therefore, the biomaterial tested was found to be favorable to fill bone defects for the reporting period analyzed.
Descritores: Crânio/efeitos dos fármacos
Cicatrização/efeitos dos fármacos
Regeneração Óssea/efeitos dos fármacos
Substitutos Ósseos/farmacologia
Hidroxiapatitas/farmacologia
-Crânio
Crânio/patologia
Fatores de Tempo
Cicatrização/fisiologia
Regeneração Óssea/fisiologia
Imuno-Histoquímica
Densidade Óssea
Osteocalcina/análise
Resultado do Tratamento
Ratos Wistar
Substitutos Ósseos/uso terapêutico
Fator A de Crescimento do Endotélio Vascular/análise
Subunidade alfa 1 de Fator de Ligação ao Core/análise
Fosfatase Ácida Resistente a Tartarato/análise
Hidroxiapatitas/uso terapêutico
Limites: Animais
Masculino
Tipo de Publ: Estudo de Avaliação
Responsável: BR1.1 - BIREME


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Id: biblio-975883
Autor: Kim, Miri; Kim, Soojung; Ko, Hyunjung; Song, Minju.
Título: Effect of ProRoot MTA® and Biodentine® on osteoclastic differentiation and activity of mouse bone marrow macrophages
Fonte: J. appl. oral sci;27:e20180150, 2019. graf.
Idioma: en.
Projeto: National Research Foundation of Korea (NRF).
Resumo: Abstract Objectives This investigation aimed to assess the differentiation inhibitory effects of ProRoot MTA® (PMTA) and Biodentine® (BIOD) on osteoclasts originated from murine bone marrow macrophages (BMMs) and compare these effects with those of alendronate (ALD). Materials and Methods Mouse BMMs were cultured to differentiate into osteoclasts with macrophage colony-stimulating factor and receptor activator of NF-κB (RANKL), treated with lipopolysaccharide. After application with PMTA, BIOD, or ALD, cell toxicities were examined using WST-1 assay kit, and RANKL-induced osteoclast differentiation and activities were determined by resorption pit formation assay and tartrate-resistant acid phosphate (TRAP) staining. The mRNA levels of osteoclast activity-related genes were detected with quantitative real time polymerase chain reaction. Expressions of molecular signaling pathways were assessed by western blot. All data were statistically analyzed with one-way ANOVA and Tukey's post-hoc test (p<0.05). Results Mouse BMMs applied with PMTA, BIOD, or ALD showed highly reduced levels of TRAP-positive osteoclasts. The BIOD treated specimens suppressed mRNA expressions of cathepsin K, TRAP, and c-Fos. Nonetheless, it showed a lower effect than PMTA or ALD applications. Compared with ALD, PMTA and BIOD decreased RANKL-mediated phosphorylation of ERK1/2 and IκBα. Conclusions PMTA and BIOD showed the inhibitory effect on osteoclast differentiation and activities similar to that of ALD through IκB phosphorylation and suppression of ERK signaling pathways.
Descritores: Osteoclastos/efeitos dos fármacos
Materiais Restauradores do Canal Radicular/farmacologia
Células da Medula Óssea/efeitos dos fármacos
Diferenciação Celular/efeitos dos fármacos
Silicatos/farmacologia
Compostos de Cálcio/farmacologia
Alendronato/farmacologia
Conservadores da Densidade Óssea/farmacologia
-Osteoclastos/fisiologia
Osteogênese/efeitos dos fármacos
Fosforilação/efeitos dos fármacos
Reabsorção da Raiz/prevenção & controle
Fatores de Tempo
Células da Medula Óssea/citologia
Sobrevivência Celular/efeitos dos fármacos
Células Cultivadas
Western Blotting
Reprodutibilidade dos Testes
Sistema de Sinalização das MAP Quinases/efeitos dos fármacos
Proteínas I-kappa B/efeitos dos fármacos
Ligante RANK/análise
Ligante RANK/efeitos dos fármacos
Reação em Cadeia da Polimerase em Tempo Real
Fosfatase Ácida Resistente a Tartarato
Limites: Animais
Camundongos
Tipo de Publ: Estudo de Avaliação
Responsável: BR1.1 - BIREME


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Id: biblio-951984
Autor: Martins, Christine Men; Sasaki, Hajime; Hirai, Kimito; Andrada, Ana Cristina; Gomes-Filho, João Eduardo.
Título: Relationship between hypertension and periapical lesion: an in vitro and in vivo study
Fonte: Braz. oral res. (Online);30(1):e78, 2016. graf.
Idioma: en.
Projeto: FAPESP.
Resumo: Abstract The aim of this study was to compare potential aspects of periapical lesion formation in hypertensive and normotensive conditions using hypertensive (BPH/2J) and wild-type control (BPN/3J) mice. The mandibular first molars of both strains had their dental pulp exposed. At day 21 the mice were euthanized and right mandibular molars were used to evaluate the size and phenotype of apical periodontitis by microCT. Proteins were extracted from periapical lesion on the left side and the expressions of IL1α, IL1β and TNFα were analyzed by ELISA. Bone marrow stem cells were isolated from adult mice femurs from 2 strains and osteoclast differentiation was evaluated by tartrate-resistant acid phosphatase (TRAP) in vitro. The amount of differentiated osteoclastic cells was nearly double in hypertensive mice when compared to the normotensive strain (p < 0.03). Periapical lesion size did not differ between hypertensive and normotensive strains (p > 0.7). IL1α, IL1β and TNFα cytokines expressions were similar for both systemic conditions (p > 0.05). Despite the fact that no differences could be observed in periapical lesion size and cytokines expressions on the systemic conditions tested, hypertension showed an elevated number of osteoclast differentiation.
Descritores: Doenças Periapicais/patologia
Células da Medula Óssea/patologia
Ligante RANK/análise
Hipertensão/patologia
-Doenças Periapicais/etiologia
Valores de Referência
Fatores de Tempo
Ensaio de Imunoadsorção Enzimática
Fator de Necrose Tumoral alfa/análise
Interleucina-1alfa/análise
Interleucina-1beta/análise
Microtomografia por Raio-X
Fosfatase Ácida Resistente a Tartarato
Hipertensão/complicações
Limites: Animais
Masculino
Feminino
Camundongos
Responsável: BR1.1 - BIREME


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Id: biblio-952053
Autor: Kim, Taegun; Lee, Won; Baek, Sang-Ho; Pyo, Sungwoon; Kook, Yoon-Ah; Bayome, Mohamed; Kim, Insoo.
Título: Effects of alveolar bone displacement with segmental osteotomy: micro-CT and histomorphometric analysis in rats
Fonte: Braz. oral res. (Online);30(1):e132, 2016. tab, graf.
Idioma: en.
Resumo: Abstract The purpose of this study was to evaluate the effects of segmental osteotomy on the blood vessels and osteoclasts in rats using micro-computed tomography (micro-CT) and histomorphometric analysis. After segmental osteotomy was performed around the maxillary first molars of 36 male Sprague-Dawley rats (n = 72), the samples were divided into a control group (no displacement), 0.5 D group (0.5 mm buccal displacement) and 1.0 D group (1.0 mm buccal displacement) (n = 24/group). At 1, 2, 4 and 8 weeks after surgery, changes in the blood vessel volume were investigated using micro-CT with perfusion of radiopaque silicone rubber. Tartrate-resistant acid phosphatase (TRAP) staining was used for histomorphometric analysis. Two-way repeated measures analysis of variance (rmANOVA) was performed to compare the volume of blood vessels and number of TRAP-positive osteoclasts among the groups. Regarding blood vessel volume, the displacement groups had no significant effects, while the time points had significant effects (p = 0.014). The blood vessel volume at 1 week was significantly smaller than that at 2, 4, and 8 weeks (p = 0.004, p = 0.026, and p = 0.005, respectively). Regarding TRAP cell count, the displacement groups had no significant effects, while the time points had significant effects (p < 0.001). The number of TRAP-positive osteoclasts at 8 weeks was significantly smaller than that at 1, 2, and 4 weeks (p < 0.001, p < 0.001, and p = 0.002, respectively), and the count at 4 weeks was smaller than that at 1 week (p = 0.011). Therefore, a regional osteoclast-related acceleratory phenomenon was maintained until 4 weeks after surgery.
Descritores: Processo Alveolar/irrigação sanguínea
Alveolectomia/métodos
Osteotomia Maxilar/métodos
-Osteoclastos
Valores de Referência
Fatores de Tempo
Contagem de Células
Reprodutibilidade dos Testes
Ratos Sprague-Dawley
Microtomografia por Raio-X
Processo Alveolar/diagnóstico por imagem
Fosfatase Ácida Resistente a Tartarato
Dente Molar
Limites: Animais
Masculino
Ratos
Tipo de Publ: Estudo de Avaliação
Responsável: BR1.1 - BIREME


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Tanaka, Orlando Motohiro
Camargo, Elisa Souza
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Id: biblio-974455
Autor: Araujo, Cristiano Miranda de; Rocha, Adriana Cristina; Araujo, Bianca Marques de Mattos de; Johann, Aline Cristina Batista Rodrigues; Pereira, Luiz Fernando; Tanaka, Orlando Motohiro; Guariza Filho, Odilon; Camargo, Elisa Souza.
Título: Effect of acute administration of nicotine and ethanol on tooth movement in rats
Fonte: Braz. oral res. (Online);32:e96, 2018. tab, graf.
Idioma: en.
Resumo: Abstract The aim of this study was to evaluate the effect of acute administration of nicotine and ethanol on tooth movement in rats. Two hundred rats were divided into eight groups: S: saline; N: nicotine; E: ethanol; NE: nicotine and ethanol; SM: saline with tooth movement; NM: nicotine with tooth movement; EM: ethanol with tooth movement; and NEM: nicotine and ethanol with tooth movement. All the solutions were applied for 32, 44, or 58 days, according to the subgroup. Orthodontic movement (25 cN) was initiated 30 days after solution administration in the groups with tooth movement. The rats were euthanized 2, 14, or 28 days after initiation of tooth movement. Tooth sections were stained using picrosirius and tartrate-resistant acid phosphatase (TRAP). The data were compared by ANOVA using Tukey's HSD and Games-Howell. On day 28 of tooth movement, the NEM group had a lower percentage of type I collagen compared to the SM group (p = 0.0448), and the S group had a higher number of osteoclasts/μm2 compared to the N group (p = 0.0405). Nicotine and ethanol did not affect the tooth movement rate, regardless of induction of orthodontic movement. Nicotine influenced the number of osteoclasts by decreasing their quantity when dental movement was not induced. When nicotine was associated with ethanol, it interfered in the maturation of collagen fibers during orthodontic movement.
Descritores: Técnicas de Movimentação Dentária/métodos
Regeneração Óssea/efeitos dos fármacos
Reabsorção Óssea/induzido quimicamente
Etanol/administração & dosagem
Processo Alveolar/efeitos dos fármacos
Nicotina/administração & dosagem
-Osteoclastos/efeitos dos fármacos
Osteogênese/efeitos dos fármacos
Valores de Referência
Fatores de Tempo
Distribuição Aleatória
Colágeno/efeitos dos fármacos
Ratos Wistar
Fosfatase Ácida Resistente a Tartarato
Limites: Animais
Masculino
Tipo de Publ: Estudo de Avaliação
Responsável: BR1.1 - BIREME


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Id: biblio-989482
Autor: Toker, Hulya; Yuce, Hatice BALCI; Yildirim, Ali; Bugrul Tekin, Mehmet; Gevrek, Fikret.
Título: The effect of colchicine on alveolar bone loss in ligature-induced periodontitis
Fonte: Braz. oral res. (Online);33:e001, 2019. graf.
Idioma: en.
Resumo: Abstract Colchicine is widely used in the treatment of several inflammatory diseases due to its anti-inflammatory effect, but effects on bone metabolism are unclear. The aim of this study was to evaluate the effects of systemically-administered colchicine on healthy periodontium and experimentally-induced periodontitis. In total, 42 male Wistar rats were included in this study. A non-ligated group constituting the negative control group (Control, C, n = 6) and a ligature-only group forming the positive control group (LO, n = 12) were created separately. Twelve rats were treated with 0.4 mg/kg colchicine and another 12 with 1 mg/kg colchicine. In the colchicine-administered groups, right mandibles constituted the ligated groups (1 mgC-L or 0.4 mgC-L) and left mandibles formed the corresponding non-ligated controls (1mgC or 0.4mgC). Silk ligatures were placed at the gingival margin of the lower first molars. The animals were euthanized at different time-points of healing (11 or 30 days). Alveolar bone loss was clinically measured and TRAP+ osteoclasts, osteoblastic activity, and MMP-1 expression were examined histologically. There was no increase in alveolar bone loss with either colchicine dose in healthy periodontium (p > 0.05) and the highest level of alveolar bone loss, TRAP+ osteoclast number, and MMP-1 expression were measured in the LO group (p < 0.05). The 0.4 mgC-L group showed less alveolar bone loss at 11 days (p < 0.05), but greater loss at 30 days. The 1 mgC-L group showed higher osteoblast number than the other ligated groups (p < 0.05) at both time-points. In summary, colchicine did not increase alveolar bone loss in healthy periodontium and also may tend to reduce periodontitis progression. However, further extensive study is necessary to understand the mechanism of colchicine action on alveolar bone loss in periodontitis.
Descritores: Periodontite/tratamento farmacológico
Colchicina/farmacologia
Perda do Osso Alveolar/tratamento farmacológico
Anti-Inflamatórios/farmacologia
-Osteoblastos/efeitos dos fármacos
Osteoclastos/efeitos dos fármacos
Periodontite/etiologia
Periodontite/patologia
Fatores de Tempo
Imuno-Histoquímica
Colchicina/uso terapêutico
Reprodutibilidade dos Testes
Perda do Osso Alveolar/patologia
Resultado do Tratamento
Ratos Wistar
Metaloproteinase 1 da Matriz/análise
Moduladores de Tubulina/farmacologia
Fosfatase Ácida Resistente a Tartarato/análise
Ligadura
Anti-Inflamatórios/uso terapêutico
Limites: Humanos
Animais
Masculino
Tipo de Publ: Estudo de Avaliação
Responsável: BR1.1 - BIREME


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Id: biblio-1089395
Autor: Souza, João Antonio Chaves de; Magalhães, Fernando Augusto Cintra; Oliveira, Guilherme Jose Pimentel Lopes de; De Molon, Rafael Scaf; Zuanon, José Antonio; Souza, Pedro Paulo Chaves de.
Título: Pam2CSK4 (TLR2 agonist) induces periodontal destruction in mice
Fonte: Braz. oral res. (Online);34:e012, 2020. graf.
Idioma: en.
Projeto: Fundação de Amparo à Pesquisa do Estado de São Paulo; . Fundação de Amparo à Pesquisa do Estado de São Paulo.
Resumo: Abstract Lipoproteins are important bacterial immunostimulating molecules capable of inducing receptor activator of nuclear factor-κB (RANKL) and osteoclast formation in vitro and in vivo . Although these molecules are present in periodontopathogenic bacteria, their role in periodontitis is not known. In this study, we used Pam2CSK4 (PAM2), a synthetic molecule that mimics bacterial lipoprotein, to investigate the effects of lipoproteins on periodontitis in mice. C57BL/6 male mice were randomly divided into three experimental groups: 1) Negative control group: animals received vehicle injection; 2) Positive control group: animals received injection of Escherichia coli lipopolysaccharide (LPS); 3) PAM2 group: animals received PAM2 injection. All the injections were performed bilaterally every other day into the palatal mucosa between first and second molars. After twenty-four days, the animals were euthanized to assess alveolar bone volume (micro-CT), cellular and extracellular composition in the gingiva (stereometric analysis), and osteoclast numbers (TRAP staining). Treatment with either PAM2 or LPS induced gingival inflammation, as demonstrated by increased infiltration of inflammatory cells and enhanced angiogenesis, associated with a smaller number of fibroblasts and decreased extracellular matrix. Importantly, treatment not only with LPS but also with PAM2 resulted in a larger number of TRAP+ multinucleated osteoclasts and significant loss of alveolar bone. Collectively, our data demonstrate that PAM2 can induce gingival inflammation and bone loss in mice, broadening the avenues of investigation into the role of lipoproteins in the pathogenesis of periodontal disease.
Descritores: Periodontite/etiologia
Periodontite/patologia
Receptor 2 Toll-Like/antagonistas & inibidores
Lipopeptídeos/farmacologia
-Osteoclastos/efeitos dos fármacos
Osteoclastos/fisiologia
Periodontite/microbiologia
Fatores de Tempo
Distribuição Aleatória
Perda do Osso Alveolar/etiologia
Perda do Osso Alveolar/patologia
Modelos Animais de Doenças
Microtomografia por Raio-X
Processo Alveolar/efeitos dos fármacos
Processo Alveolar/patologia
Fosfatase Ácida Resistente a Tartarato
Gengiva/efeitos dos fármacos
Gengiva/patologia
Gengivite/etiologia
Gengivite/patologia
Camundongos Endogâmicos C57BL
Limites: Animais
Masculino
Responsável: BR1.1 - BIREME


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Id: biblio-1055530
Autor: Department of PeriodontologySOUZA, João Antonio Chaves de; MAGALHÃES, Fernando Augusto Cintra; Department of PeriodontologyOLIVEIRA, Guilherme Jose Pimentel Lopes de; Department of Diagnosis and SurgeryDE MOLON, Rafael Scaf; Department of Physiology and PathologyZUANON, José Antonio; Department of StomatologySOUZA, Pedro Paulo Chaves de.
Título: Pam2CSK4 (TLR2 agonist) induces periodontal destruction in mice
Fonte: Braz. oral res. (Online);34:e012, 2020. graf.
Idioma: en.
Projeto: Fundação de Amparo à Pesquisa do Estado de São Paulo; . Fundação de Amparo à Pesquisa do Estado de São Paulo.
Resumo: Abstract Lipoproteins are important bacterial immunostimulating molecules capable of inducing receptor activator of nuclear factor-κB (RANKL) and osteoclast formation in vitro and in vivo . Although these molecules are present in periodontopathogenic bacteria, their role in periodontitis is not known. In this study, we used Pam2CSK4 (PAM2), a synthetic molecule that mimics bacterial lipoprotein, to investigate the effects of lipoproteins on periodontitis in mice. C57BL/6 male mice were randomly divided into three experimental groups: 1) Negative control group: animals received vehicle injection; 2) Positive control group: animals received injection of Escherichia coli lipopolysaccharide (LPS); 3) PAM2 group: animals received PAM2 injection. All the injections were performed bilaterally every other day into the palatal mucosa between first and second molars. After twenty-four days, the animals were euthanized to assess alveolar bone volume (micro-CT), cellular and extracellular composition in the gingiva (stereometric analysis), and osteoclast numbers (TRAP staining). Treatment with either PAM2 or LPS induced gingival inflammation, as demonstrated by increased infiltration of inflammatory cells and enhanced angiogenesis, associated with a smaller number of fibroblasts and decreased extracellular matrix. Importantly, treatment not only with LPS but also with PAM2 resulted in a larger number of TRAP+ multinucleated osteoclasts and significant loss of alveolar bone. Collectively, our data demonstrate that PAM2 can induce gingival inflammation and bone loss in mice, broadening the avenues of investigation into the role of lipoproteins in the pathogenesis of periodontal disease.
Descritores: Periodontite/etiologia
Periodontite/patologia
Receptor 2 Toll-Like/antagonistas & inibidores
Lipopeptídeos/farmacologia
-Osteoclastos/efeitos dos fármacos
Periodontite/microbiologia
Fatores de Tempo
Distribuição Aleatória
Perda do Osso Alveolar/etiologia
Perda do Osso Alveolar/patologia
Modelos Animais de Doenças
Microtomografia por Raio-X
Processo Alveolar/efeitos dos fármacos
Processo Alveolar/patologia
Fosfatase Ácida Resistente a Tartarato
Gengiva/efeitos dos fármacos
Gengiva/patologia
Gengivite/etiologia
Gengivite/patologia
Camundongos Endogâmicos C57BL
Limites: Animais
Masculino
Responsável: BR1.1 - BIREME



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