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Id: biblio-827438
Autor: Fuchs, Leandro; Duarte, Danielle Walter; Gaissler, Vanessa.
Título: Continuous absorbable suture versus interrupted permanent suture in the treatment of diastasis of the rectus abdominis muscle / Sutura contínua com fio absorvível versus interrompida com fio permanente no tratamento da diástase do músculo reto abdominal
Fonte: Rev. bras. cir. plást;31(4):516-521, 2016. ilus, tab.
Idioma: en; pt.
Resumo: Introduction: Plication of the rectus abdominis muscle is an important step in remodeling the abdomen in abdominoplasties. It can be performed using several types of threads with interrupted or continuous sutures, according to the surgeon's preferences. Few studies in the literature compared the different plication techniques in abdominoplasties. The present study aimed to compare continuous anchored suture with polydioxanone (PDS) with interrupted nylon suture regarding surgical time, relapse, and costs and discuss the advantages and disadvantages of each method. Methods: A randomized clinical trial in which 30 patients were enrolled for each of the two groups, 15 with continuous suture and 15 with interrupted suture, was conducted. The plication time was compared between the groups using the Student's t-test. Between 10 and 12 months postoperatively, all patients underwent ultrasonography for investigating diastasis recurrence. Results: Continuous suture decreased the plication time by almost 40% when compared to the interrupted suture (p < 0.001), without affecting the esthetic outcome. The PDS used in the continuous suture implied higher costs than those with the nylon threads used in the interrupted suture. No recurrence was found on the postoperative control ultrasonography results. Conclusion: Continuous suture reduced the plication time by almost 40% (p < 0.001) when compared to interrupted suture. Despite being associated with higher costs, PDS seems to be a good alternative to non-absorbable sutures, such as nylon threads, in plications of the rectus abdominis, since it is a more biocompatible material.

Introdução: A plicatura do músculo reto abdominal é um passo importante no remodelamento do abdômen nas abdominoplastias. Ela pode ser realizada com vários tipos de fios e com suturas interrompidas ou contínuas, de acordo com as preferências do cirurgião. Poucos estudos na literatura compararam diferentes técnicas de plicatura nas abdominoplastias. O objetivo do estudo é comparar a sutura contínua ancorada com polidioxanona (PDS) com a sutura interrompida com náilon quanto ao tempo cirúrgico, recidiva, custos e discutir vantagens de desvantagens de cada método. Métodos: Ensaio clínico randomizado no qual 30 pacientes foram arroladas para cada um dos grupos, 15 sutura contínua e 15 sutura interrompida. O tempo de plicatura foi comparado entre os grupos utilizando-se o teste t de Student. Entre 10 a 12 meses de pós-operatório, todas as pacientes foram submetidas à ecografia em busca de recidivas da diástase. Resultados: A sutura contínua diminuiu em quase 40% o tempo de plicatura quando comparada à sutura interrompida (p < 0,001), sem prejuízo no resultado estético. O PDS utilizado na sutura contínua implicou maiores custos que os fios de náilon utilizados na sutura interrompida. Nenhuma recidiva foi encontrada na ecografia de controle no pós-operatório. Conclusão: A sutura contínua quando comparada à sutura interrompida foi capaz de reduzir o tempo de plicatura em quase 40% (p < 0,001). A utilização do PDS, apesar dos maiores custos, parece ser uma boa alternativa às suturas com fios não absorvíveis como o náilon nas plicaturas do músculo reto abdominal, uma vez que se trata de um fio de maior biocompatibilidade.
Descritores: Recidiva
Suturas
Técnicas de Sutura
Ensaio Clínico Controlado Aleatório
Polidioxanona
Reto do Abdome
Abdome
Amilases
Nylons
-Materiais Biocompatíveis
Materiais Biocompatíveis/análise
Materiais Biocompatíveis/uso terapêutico
Técnicas de Sutura/normas
Polidioxanona/uso terapêutico
Reto do Abdome/cirurgia
Abdominoplastia
Abdominoplastia/métodos
Abdome/cirurgia
Amilases/uso terapêutico
Nylons/análise
Nylons/normas
Limites: Humanos
Feminino
Pessoa de Meia-Idade
Idoso
História do Século XXI
Tipo de Publ: Estudo Comparativo
Revisão
Ensaio Clínico Controlado Aleatório
Responsável: BR32.1 - Serviço de Biblioteca e Informação Biomédica


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Id: biblio-1087698
Autor: Liu, Shu; Ahmed, Sibtain; Zhang, Chunguang; Liu, Tongxiao; Shao, Changlun; Fang, Yaowei.
Título: Diversity and antimicrobial activity of culturable fungi associated with sea anemone Anthopleura xanthogrammica
Fonte: Electron. j. biotechnol;44:41-46, Mar. 2020. tab, ilus.
Idioma: en.
Projeto: National Natural Science Foundation of China; . Jiangsu Province Marine Science and Technology Innovation Project; . Priority Academic Program Development of Jiangsu Higher Education; . Institutions, Six Talent Peaks Project in Jiangsu Province.
Resumo: Background: The main objective of this study was to isolate fungi associated with Anthopleura xanthogrammica and measure their antimicrobial and enzymatic activities. A total of 93 fungal strains associated with A. xanthogrammica were isolated in this study, of which 32 isolates were identified using both morphological characteristics and internal transcribed spacer (ITS) sequence analysis. The antibacterial activities of 32 fungal isolates were tested against Bacillus subtilis, Staphylococcus aureus, Escherichia coli, Edwardsiella tarda, Vibrio harveyi, Fusarium oxysporum, and Pyricularia oryzae by agar diffusion assay. Extracellular hydrolytic enzyme activities of the fungal isolates were determined by agar diffusion assays. Enzyme activities were detected from clear halo size. Results: The isolated fungi belonged to 18 genera within 7 taxonomic orders of 1 phylum. The genera Aspergillaceae were the most diverse and common. The antimicrobial activities of 32 isolates were evaluated, and 19 (59.4%) of fungi isolate displayed unique antimicrobial activities. All fungal strains displayed at least one enzyme activity. The most common enzyme activities in the fungi isolates were amylase and protease, while the least common were pectinase and xylanase. Conclusions: This is first report on the sea anemone-derived fungi with antimicrobial and enzyme activities. Results indicated that sea anemone is a hot spot of fungal diversity and a rich resource of bioactive natural products.
Descritores: Aspergillus/isolamento & purificação
Anêmonas-do-Mar/microbiologia
Antibacterianos/isolamento & purificação
-Peptídeo Hidrolases/metabolismo
Filogenia
Poligalacturonase/metabolismo
Aspergillus/enzimologia
Aspergillus/genética
Bactérias/efeitos dos fármacos
DNA Espaçador Ribossômico
Biodiversidade
Fungos/isolamento & purificação
Fungos/genética
Amilases/metabolismo
Antibacterianos/farmacologia
Responsável: CL1.1 - Biblioteca Central


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Id: biblio-949368
Autor: Qian, Yunzhong; Chen, Yi; Wang, Linyang; Tou, Jinfa.
Título: Effects of baicalin on inflammatory reaction, oxidative stress and PKDl and NF-kB protein expressions in rats with severe acute pancreatitis
Fonte: Acta cir. bras;33(7):556-564, July 2018. tab, graf.
Idioma: en.
Resumo: Abstract Purpose: To investigate the effects of baicalin on inflammatory reaction, oxidative stress and protein kinase D1 (PKD1) and nuclear factor-kappa B (NF-κB) protein expressions in severe acute pancreatitis (SAP) rats. Methods: Sixty rats were divided into sham operation, model, and low-, medium- and high-dose baicalin group. SAP model was established in later 4 groups. The later 3 groups were injected with 0.1, 0.2 and 0.4 ml/100 g 5% baicalin injection, respectively. At 12 h, the serum SAP related indexes and inflammatory factors, peripheral blood CD3 and γδT cell percentages, wet/dry ratio and pancreas ascites volume, oxidative stress indexes and PKD1 and NF-κB protein expressions in pancreatic tissue were determined. Results: Compared with model group, in high-dose baicalin group the wet/dry ratio and ascites volume, serum amylase level, phospholipase A2 activity, TNF-α, IL-1 and IL-6 levels, and pancreatic malondialdehyde level and PKD1 and NF-κB protein expression were significantly decreased (P < 0.05), and peripheral blood CD3 and γδT cell percentages and pancreatic superoxide dismutase and glutathione peroxidase levels were significantly increased (P < 0.05). Conclusion: Baicalin can resist the inflammatory reaction and oxidative stress, and down-regulate protein kinase D1 and nuclear factor-kappa B protein expressions, thus exerting the protective effects on severe acute pancreatitis in rats.
Descritores: Pancreatite/tratamento farmacológico
Flavonoides/farmacologia
Proteína Quinase C/metabolismo
Anti-Inflamatórios não Esteroides/farmacologia
NF-kappa B/metabolismo
Estresse Oxidativo/efeitos dos fármacos
-Pancreatite/metabolismo
Superóxido Dismutase/efeitos dos fármacos
Proteína Quinase C/efeitos dos fármacos
Distribuição Aleatória
Regulação para Baixo/efeitos dos fármacos
Reprodutibilidade dos Testes
NF-kappa B/efeitos dos fármacos
Interleucina-6/sangue
Interleucina-1/sangue
Fator de Necrose Tumoral alfa/sangue
Resultado do Tratamento
Ratos Sprague-Dawley
Complexo CD3/efeitos dos fármacos
Complexo CD3/sangue
Glutationa Peroxidase/efeitos dos fármacos
Glutationa Peroxidase/metabolismo
Amilases/efeitos dos fármacos
Amilases/sangue
Malondialdeído/metabolismo
Limites: Animais
Tipo de Publ: Estudo de Avaliação
Responsável: BR1.1 - BIREME


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Id: lil-788963
Autor: Blanco, Alina Sánchez; Durive, Osmar Palacios; Pérez, Sulema Batista; Montes, Zoraida Díaz; Guerra, Nelson Pérez.
Título: Simultaneous production of amylases and proteases by Bacillus subtilis in brewery wastes
Fonte: Braz. j. microbiol;47(3):665-674, July-Sept. 2016. tab, graf.
Idioma: en.
Resumo: ABSTRACT The simultaneous production of amylase (AA) and protease (PA) activity by Bacillus subtilis UO-01 in brewery wastes was studied by combining the response surface methodology with the kinetic study of the process. The optimum conditions (T = 36.0 °C and pH = 6.8) for high biomass production (0.92 g/L) were similar to the conditions (T = 36.8 °C and pH = 6.6) for high AA synthesis (9.26 EU/mL). However, the maximum PA level (9.77 EU/mL) was obtained at pH 7.1 and 37.8 °C. Under these conditions, a considerably high reduction (between 69.9 and 77.8%) of the initial chemical oxygen demand of the waste was achieved. In verification experiments under the optimized conditions for production of each enzyme, the AA and PA obtained after 15 h of incubation were, respectively, 9.35 and 9.87 EU/mL. By using the Luedeking and Piret model, both enzymes were classified as growth-associated metabolites. Protease production delay seemed to be related to the consumption of non-protein and protein nitrogen. These results indicate that the brewery waste could be successfully used for a high scale production of amylases and proteases at a low cost.
Descritores: Peptídeo Hidrolases/biossíntese
Bacillus subtilis/metabolismo
Fermentação
Amilases/biossíntese
Resíduos Industriais
-Temperatura
Cinética
Biotransformação
Metabolismo dos Carboidratos
Concentração de Íons de Hidrogênio
Responsável: BR1.1 - BIREME


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Id: biblio-827647
Autor: Tang, Maochun; Zong, Pengfei; Zhang, Ting; Wang, Dongyan; Wang, Yuhui; Zhao, Yan.
Título: Lipoprotein lipase gene-deficient mice with hypertriglyceridaemia associated with acute pancreatitis
Fonte: Acta cir. bras;31(10):655-660, Oct. 2016. tab, graf.
Idioma: en.
Resumo: ABSTRACT PURPOSE: To investigate the severity of pancreatitis in lipoprotein lipase (LPL)-deficient hypertriglyceridaemic (HTG) heterozygous mice and to establish an experimental animal model for HTG pancreatitis study. METHODS: LPL-deficient HTG heterozygous mice were rescued by somatic gene transfer and mated with wild-type mice. The plasma amylase, triglyceride, and pathologic changes in the pancreas of the LPL-deficient HTG heterozygous mice were compared with those of wild-type mice to assess the severity of pancreatitis. In addition, acute pancreatitis (AP) was induced by caerulein (50 µg/kg) for further assessment. RESULTS: The levels of plasma amylase and triglyceride were significantly higher in the LPL-deficient HTG heterozygous mice. According to the pancreatic histopathologic scores, the LPL-deficient HTG heterozygous mice showed more severe pathologic damage than the wild-type mice. CONCLUSIONS: Lipoprotein lipase deficient heterozygous mice developed severe caerulein-induced pancreatitis. In addition, their high triglyceride levels were stable. Therefore, LPL-deficient HTG heterozygous mice are a useful experimental model for studying HTG pancreatitis.
Descritores: Pancreatite/etiologia
Hipertrigliceridemia/complicações
Hiperlipoproteinemia Tipo I/complicações
-Pancreatite/patologia
Fatores de Tempo
Triglicerídeos/sangue
Índice de Gravidade de Doença
Doença Aguda
Modelos Animais de Doenças
Heterozigoto
Amilases/sangue
Hiperlipoproteinemia Tipo I/genética
Camundongos Endogâmicos C57BL
Limites: Animais
Feminino
Responsável: BR1.1 - BIREME


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Id: lil-785012
Autor: Yılmaz, Edip Erdal; Bozdağ, Zübeyir; Ibiloğlu, Ibrahim; Arıkanoğlu, Zülfü; Yazgan, Ümit Can; Kaplan, Ibrahim; Gümüş, Metehan; Atamanalp, Sabri Selçuk.
Título: Therapeutic effects of ellagic acid on L-arginin induced acute pancreatitis
Fonte: Acta cir. bras;31(6):396-401tab, graf.
Idioma: en.
Resumo: ABSTRACT PURPOSE: To investigate the therapeutic effects of ellagic acid on L-arginin ınduced acute pancreatitis in rats. METHODS: Thirty-two were split into four groups. Group 1 (control) rats were performed only laparotomy, no drugs were administered. Group 2 (control+EA) rats were administered 85mg/kg EA orally. Rats were sacrificed by cardiac puncture 24 hours after the administration. Group3 (AP) 24 hours after intraperitoneal L-arginine administration, rats were sacrificed by cardiac puncture. Group 4 (EA)-(AP): 85mg/kg EA was administered orally after the L-arginine administration. 24 hours later, rats were sacrificed by cardiac puncture. Serum TNF-α, IL-1β, IL-6, total oxidative status (TOS), total antioxidant capacity (TAC), amylase levels were determined in all groups. RESULTS: Group 3 (AP) rats showed significantly raised TOS level as compared to Group1 (control) rats (p<0.001). Following the EA therapy, a decrease in TOS was observed in Group 4 (AP+EA). TAC levels were significantly raised in the Group 4 (AP+EA) compared to the Group 3 (AP) (p=0.003). Group 3 (AP) showed significantly increased TNF-α, IL-1β and IL-6 serum levels as compared to Group 4 (AP+EA). Histopathological changes were supported our result. CONCLUSION: The healing effects of ellagic acid on inflammatory and oxidative stress were confirmed by histopathological and biochemical evaluations of the pancreatic tissue.
Descritores: Pancreatite/tratamento farmacológico
Ácido Elágico/uso terapêutico
Anti-Inflamatórios/uso terapêutico
Antioxidantes/uso terapêutico
-Pancreatite/induzido quimicamente
Pancreatite/patologia
Pancreatite/sangue
Arginina
Distribuição Aleatória
Doença Aguda
Interleucina-6/sangue
Fator de Necrose Tumoral alfa/sangue
Ratos Sprague-Dawley
Estresse Oxidativo/efeitos dos fármacos
Ácido Elágico/farmacologia
Interleucina-1beta/sangue
Amilases/efeitos dos fármacos
Amilases/sangue
Anti-Inflamatórios/farmacologia
Antioxidantes/farmacologia
Limites: Animais
Masculino
Responsável: BR1.1 - BIREME


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Id: biblio-1022044
Autor: Revin, Victor; Atykyan, Nelli; Lyovina, Ekaterina; Dragunova, Yuliya; Ushkina, Victoriya.
Título: Effect of ultraviolet radiation on physiological and biochemical properties of yeast Saccharomyces cerevisiae during fermentation of ultradispersed starch raw material
Fonte: Electron. j. biotechnol;31:61-66, Jan. 2018. graf, ilus, tab.
Idioma: en.
Projeto: Ministry of Education and Science of the Russian Federation.
Resumo: Background: Study of correlation between pretreatment of yeast with ultraviolet radiation and efficiency of further fermentation of wort made of ultrafine grain particles to ethanol. Results: We investigated three races of industrial yeast Saccharomyces cerevisiae (native and irradiated by ultraviolet). Physiological properties during fermentation of starchy wort were tested in all variants. It was shown that activation of the yeast by ultraviolet radiation allows to further increase the ethanol yield by 25% on average compared with the native yeast races when using thin (up to micro- and nano-sized particles) or standard grain grinding. Conclusions: Using mechanical two-stage grinding of starchy raw materials and ultraviolet pretreatment of yeast, the efficiency of saccharification of starch and fermentation of wort to ethanol was increased.
Descritores: Saccharomyces cerevisiae/efeitos da radiação
Raios Ultravioleta
Leveduras/efeitos da radiação
Etanol/efeitos da radiação
-Saccharomyces/metabolismo
Amido
Temperatura
Leveduras/metabolismo
Estabilidade Enzimática
Etanol/metabolismo
Fermentação
Glucose
Amilases
Responsável: CL1.1 - Biblioteca Central


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Id: biblio-966516
Autor: Haider, Muhammad Sultan; Ali, Zeeshan; Naseem, Asif; Ahmad, Muhammad; kamal, Shahid; Afzal, Muhammad.
Título: Fatty acid profile and effect of fish fermented silage on digestive enzymes in Labeo rohita / Perfil de ácidos graxos e efeito de peixe fermentado em silagem de enzimas digestivas em Labeo rohita
Fonte: Biosci. j. (Online);33(6):1562-1571, nov./dec. 2017. tab.
Idioma: en.
Resumo: Due to inconsistency in demand and supply of fishmeal there is immense need of alternate protein sources. Present project was therefore designed to replace costly fishmeal (FM) with low-priced fermented fish silage (FFS) in fish feed. Fermented fish silage was prepared by fermentation process using Lacto bacillus bacteria and its fatty acid profile and effect on digestive system of Labeo rohita was investigated. Lipid contents were isolated by Soxhlet apparatus and recorded as 6.23 ± 1.23 g/100g of fermented fish silage (FFS). Fatty acid profile of extracted Lipids was determined by gas liquid chromatography (GLC), sufficient amount of unsaturated fatty acids were found with pattern mono unsaturated fatty acids (MUFA) > saturated fatty acids (SFA) >poly unsaturated fatty acids (PUFA). Three treatment diets containing 100% silage (T1), 75% silage (T2) and 50% silage (T3) were prepared by mixing it with soybean meal (SBM) and rice bran as co-ingredients while fermented fish silage was replaced by fishmeal in control diet (T0). The experiment was conducted in glass aquaria in triplicate. Fish growth parameters were recorded fortnightly while physicochemical parameters of water were recorded on daily basis. After completion of feeding trial, three fish were randomly dissected to excise out their intestines and determine activity for protease, amylase and lipase enzymes. Non-significant differences (P<0.05) were recorded in growth parameters and enzymatic activity among all diets except lipase enzyme. Deceptively, it can be concluded that FFS has reasonable concentration of nutrients and unsaturated fatty acids so it can successfully replace fishmeal in fish diets.

Devido à diferença na procura e na oferta de farinha de peixe há imensa necessidade de qualquer membro suplente da fonte de proteína. Tão presente projeto foi projetada para substituir a dispendiosa farinha de peixe (FM) com barato peixe fermentado de ensilagem (FFS) em alimentos para peixes. FFS foi preparado pelo processo de fermentação usando Lactobacillus bactérias e seu perfil de ácidos graxos e efeito sobre o sistema digestivo de Labeo rohita foi investigado. Conteúdo lipídico foram isoladas pelo aparelho de Soxhlet e registadas como 6,23 ± 1,23 g/100g de FFS. Perfil de ácidos graxos extraídos de lipídios foi determinada por cromatografia líquida de gás (GLC). Quantidade suficiente de ácidos graxos insaturados foram encontrados com padrão MUFA > SFA > AGPI. Tratamento de três dietas contendo silagem de 100% (T1), 75% silagem (T2) e 50% silagem (T3) foram preparados misturando com farinha de soja (SBM) e farelo de arroz como co ingredientes enquanto FFS foi substituído pela FM na dieta controle (T0). O experimento foi conduzido em aquários de vidro em triplicado. O Crescimento dos peixes foram anotados os parâmetros quinzenal enquanto parâmetros físico-químicos de água foram registradas diariamente. Após a conclusão do teste de alimentação, três peixes foram aleatoriamente dissecada a impostos especiais de consumo os seus intestinos e determinar a atividade de protease, enzimas amilase e lipase. As variações não significativas (P<0,05) foi registrada em parâmetros de crescimento e atividade enzimática entre as dietas exceto enzima lipase mostrou diferença significativa entre as dietas de tratamento. Aparentemente, é possível concluir que a concentração razoável de FFS tem nutrientes e ácidos graxos insaturados de modo que ela possa substituir com êxito a farinha de peixe na dieta de peixes.
Descritores: Ácidos Graxos
Farinha de Peixe
Peixes
Amilases
Responsável: BR396.1 - Biblioteca Central


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Id: biblio-987626
Autor: Zambrano Sánchez, Gabriela; Zambrano, Javier; Mosquera Moyano, Felipe.
Título: Afectación pancreática en infección por leptospirosis / Pancreatic involvement in leptospirosis infection
Fonte: INSPILIP;1(1):1-11, ene.-jun 2017.
Idioma: es.
Resumo: La leptospirosis en fase ictérica o enfermedad de Weil es una vasculitis zoonótica endémica de la Costa ecuatoriana. Sin embargo, la pancreatitis aguda como parte de esta entidad es una complicación rara vez documentada. En este reporte de caso se presenta a un adulto varón de 63 años con signos de falla multiorgánica y amilasa con rápido ascenso inicial. La leptospirosis no fue tomada como primera opción diagnóstica. El paciente fue tratado debido a un cuadro de SDRA como un shock séptico de foco pulmonar, con falla renal aguda que requirió hemodiálisis, permaneciendo en UCI con antibioticoterapia empírica. El objetivo de este caso es remarcar que la leptospirosis debe siempre estar considerada en el diagnóstico diferencial de ictericia y pancreatitis, sobre todo en nuestro medio.

The icteric presentation of Leptospirosis, also known as Weil's disease, is a zoonotic vasculitis endemic to Ecuador's coastal region. However, reports of pancreatic affection due to this entity are rarely documented. We chose to present the case of a 63 years old male with signs of catastrophic organic failure, associated with a rapid increase in serum amylase concentrations. Due to this presentation, Leptospirosis was not held as the primary culprit of the disease, and was treated at the ICU as septic shock of pulmonary origin with ARDS associated with acute kidney injury that required hemodialysis. Thus this case aims to highlight the importance of Leptospirosis as an important differential diagnosis in any patient hailing from a tropical region with jaundice and pancreatitis.
Descritores: Vasculite
Doença de Weil
Amilases
Leptospirose
Insuficiência de Múltiplos Órgãos
-Pacientes
Vigilância Sanitária
Limites: Humanos
Masculino
Pessoa de Meia-Idade
Tipo de Publ: Relatos de Casos
Responsável: EC7.1 - Biblioteca


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Id: biblio-868808
Autor: Vargas C, Martín; Piro M, Beatriz; Navarro, A1; Rubio M, Cristina.
Título: Selección de un hongo filamentoso altamente productor de amilasas para ser usadas en detergentes biodegradables / Selection of filamentous fungus highly producer of amylases to be used in biodegradable detergents
Fonte: Bol. micol. (Valparaiso En linea);31(1):19-27, jun. 2016. ilus, tab, graf.
Idioma: es.
Resumo: Las amilasas (alfa-amilasa, EC 3.2.1.1 y glucoamilasa, EC 3.2.1.3) son enzimas extracelulares que hidrolizan el almidón en dextrinas hasta glucosa y tienen gran aplicación industrial, especialmente alimentaria; detergentes y en la producción de alcohol a partir de granos. El objetivo del trabajo es seleccionar un hongo filamentoso que presente alta producción de amilasas con características particulares para ser empleadas en biodetergentes. Se estudiaron los siguientes hongos: Penicillium expansum; P. digitatum; P. islandicum; Aspergillus clavatus; A. niger; A. ochraceus; A. fumigatus; A. flavus; A. oryzae; A. nidulans y Geotrichum candidum; Los ensayos se realizaron en un medio de hidrolizado de papa de descarte (variedad Spunta) suplementado con las siguientes sales: KH2 PO4 1,0; NaNO3 3,0; MgSO4 .7H2 O 0,5, a pH 4,0; se inoculó con 2 x106 conidios/mL y se incubaron a 25ºC en un agitador rotatorio a una velocidad de agitación de 250 rpm. Con los extractos enzimáticos parcialmente purificados con (NH4 )2 SO4 al 60 por ciento de saturación, se estudió el efecto del pH (2,5; 3,5; 4,0; 4,5; 5,0; 5,5; 6,0, 7,0 y 8,0) y la temperatura (20; 25; 30; 35 y 40ºC). Los resultados mostraron que la máxima producción de enzima (128 U/L) se obtuvo con Aspergillus niger, en las condiciones ensayadas, a las 48 h de incubación, con alto rendimiento de producto respecto a la biomasa (Yp/x= 18,3 U/g) y productividad volumétrica (Pdv=2,7 U/L). El análisis cualitativo de las enzimas del complejo amilolítico de A. niger mostró que las amilasas implicadas son alfa-amilasa y glucoamilasa y se caracterizaron por hidrolizar en un tiempo de 3 min. manchas mixtas de almidón y grasas de muestras textiles en un rango de pH 4,0 a 8,0 y de 20 a 40 ºC.

The amylases (alpha-amylase, EC 3.2.1.1 and glucoamylase, EC 3.2.1.3) are extracellular enzymes that hydrolyze starch into dextrins to glucose and have great application industrial, especially food, detergents and in the production of alcohol from grains. The objective of the study is to select a filamentous fungus that present high production of amylases showing attractive features to be used in biodetergentes. Were studied following fungus: Penicillium expansum; P. digitatum; P. islandicum; Aspergillus clavatus; A niger; A. ochraceus; A. fumigatus; A. flavus; A. oryzae; A. nidulans and Geotrichum candidum. The tests were conducted in the medium of hydrolyzed potato discard (variety Spunta) supplemented with the following sales: KH2 PO4 , 1.0; NaNO3 , 3.0 and MgSO4 .7H2 O, 0.5, to pH 4.0. Were inoculated with 2 x 106 conidia/ mL and incubated at 25 °C on a rotary Shaker at a speed of 250 rpm. With partially purified enzyme extracts with (NH4 )2 SO4 at 60 percent of saturation, we studied the effect of pH (2.5; 3.5; 4.0; 4.5; 5.0; 5.5; 6.0, 7.0 and 8.0) and temperature (20; 25; 30; 35, and 40 ° C). The results showed that the maximum production of enzyme (128 U/L) was obtained with Aspergillus niger, under the conditions tested, at 48 h of incubation, with high product formation rate with respect to biomass (Yp/x = 18.3 U/g) and volumetric productivity (Pdv = 2,7 U/ hL). The qualitative analysis of the enzymes of the complex amylolític of A. niger showed that involved amylases are α-amylase and glucoamylase and characterized by hydrolyze in 3 min spots mixed starch and fats of textile samples over a range of pH 4.0 to 8.0 and 20 to 40 ° C.
Descritores: Amilases/análise
Aspergillus niger/isolamento & purificação
Aspergillus niger/enzimologia
GLUCANO 1,ABBREVIATIONS AS TOPIC-ALFA-GLUCOSIDASA
Fungos/enzimologia
-Biodegradação Ambiental
Detergentes
Concentração de Íons de Hidrogênio
Hidrólise
Amido
Temperatura
Responsável: CL2.1 - Biblioteca de Medicina



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