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Id: |
lil-688590
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Autor: |
Gottschalk, Leda Maria Fortes; Paredes, Raquel de Sousa; Teixeira, Ricardo Sposina Sobral; Silva, Ayla Sant'Ana da; Bon, Elba Pinto da Silva. |
Título: |
Efficient production of lignocellulolytic enzymes xylanase, β-xylosidase, ferulic acid esterase and β-glucosidase by the mutant strain Aspergillus awamori 2B.361 U2/1 |
Fonte: |
Braz. j. microbiol;44(2):569-576, 2013. graf, tab.
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Idioma: |
en.
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Resumo: |
The production of xylanase, β-xylosidase, ferulic acid esterase and β-glucosidase by Aspergillus awamori 2B.361 U2/1, a hyper producer of glucoamylase and pectinase, was evaluated using selected conditions regarding nitrogen nutrition. Submerged cultivations were carried out at 30 ºC and 200 rpm in growth media containing 30 g wheat bran/L as main carbon source and either yeast extract, ammonium sulfate, sodium nitrate or urea, as nitrogen sources; in all cases it was used a fixed molar carbon to molar nitrogen concentration of 10.3. The use of poor nitrogen sources favored the accumulation of xylanase, β-xylosidase and ferulic acid esterase to a peak concentrations of 44,880; 640 and 118 U/L, respectively, for sodium nitrate and of 34,580, 685 and 170 U/L, respectively, for urea. However, the highest β-glucosidase accumulation of 10,470 U/L was observed when the rich organic nitrogen source yeast extract was used. The maxima accumulation of filter paper activity, xylanase, β-xylosidase, ferulic acid esterase and β-glucosidase by A. awamori 2B.361 U2/1 was compared to that produced by Trichoderma reesei Rut-C30. The level of β-glucosidase was over 17-fold higher for the Aspergillus strain, whereas the levels of xylanase and β-xylosidase were over 2-fold higher. This strain also produced ferulic acid esterase (170 U/L), which was not detected in the T. reesei culture.
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Descritores: |
Aspergillus/enzimologia Hidrolases de Éster Carboxílico/metabolismo Xilosidases/metabolismo beta-Glucosidase/metabolismo
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-Aspergillus/genética Aspergillus/crescimento & desenvolvimento Carbono/metabolismo Meios de Cultura/química Nitrogênio/metabolismo Temperatura
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Tipo de Publ: |
Research Support, Non-U.S. Gov't
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Responsável: |
BR1.1 - BIREME
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