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Id: biblio-1041762
Autor: Kuhar, Francisco; Castiglia, Valeria C; Zamora, Juan C.
Título: Detección de manganeso peroxidasa y otras exoenzimas en 4 aislamientos de Geastrum (Geastrales) en cultivo puro / Detection of manganese peroxidase and other exoenzymes in four isolates of Geastrum (Geastrales) in pure culture
Fonte: Rev. argent. microbiol;48(4):274-278, dic. 2016. ilus, graf, tab.
Idioma: en.
Resumo: Knowledge regarding the enzymatic machinery of fungi is decisive to understand their ecological role. The species of the genus Geastrum are known to grow extremely slowly in pure culture, which makes it difficult to evaluate physiological parameters such as enzyme activity. Qualitative assays were performed on isolates of four species of this genus, showing evidence of laccase, cellulase, pectinase, amylase and lipase activity and suggesting that a wide range of carbon sources can be exploited by these species. For the first time in this genus, quantitative assays verified manganese peroxidase activity (up to 0.6 mU/g) in 30-day old cultures, as well as laccase, β-glycosidase and β-xylosidase activities.

El conocimiento de la maquinaria enzimática de un hongo es decisivo para entender su rol ecológico. Las especies del género Geastrum son conocidas por su crecimiento extremadamente lento en cultivos puros, lo que hace difícil la evaluación de parámetros fisiológicos como las actividades enzimáticas. Se realizaron ensayos cualitativos sobre aislamientos de 4 especies de este género, mostrando evidencias de actividades lacasa, celulasa, pectinasa, amilasa y lipasa, mostrando el amplio rango de fuentes de carbono que pueden ser explotadas por estas especies. Ensayos cuantitativos verificaron por primera vez en este género la actividad manganeso peroxidasa (hasta 0,6 mU/g) en cultivos de 30 días, así como también β-glucosidasa y β-xilosidasa.
Descritores: Fungos/enzimologia
-Xilosidases/isolamento & purificação
Biotransformação/fisiologia
Celulase/isolamento & purificação
Lacase/isolamento & purificação
Fungos/fisiologia
Lipase/isolamento & purificação
Tipo de Publ: Ensaio Clínico
Estudo de Avaliação
Responsável: AR635.1 - FCVyS - Servicio de Información y Documentación


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Id: biblio-843173
Autor: Ramos, Araceli M; Gally, Marcela; Szapiro, Gala; Itzcovich, Tatiana; Carabajal, Maira; Levin, Laura.
Título: Crecimiento in vitro y producción de enzimas degradadoras de pared celular vegetal de aislamientos argentinos de Macrophomina phaseolina, agente causal de la podredumbre carbonosa en maíz / In vitro growth and cell wall degrading enzyme production by Argentinean isolates of Macrophomina phaseolina, the causative agent of charcoal rot in corn
Fonte: Rev. argent. microbiol;48(4):267-273, dic. 2016. graf, tab.
Idioma: en.
Resumo: Macrophomina phaseolina is a polyphagous phytopathogen, causing stalk rot on many commercially important species. Damages caused by this pathogen in soybean and maize crops in Argentina during drought and hot weather have increased due its ability to survive as sclerotia in soil and crop debris under non-till practices. In this work, we explored the in vitro production of plant cell wall-degrading enzymes --#91;pectinases (polygalacturonase and polymethylgalacturonase); cellulases (endoglucanase); hemicellulases (endoxylanase) and the ligninolytic enzyme laccase--#93; by several Argentinean isolates of M. phaseolina, and assessed the pathogenicity of these isolates as a preliminary step to establish the role of these enzymes in M. phaseolina-maize interaction. The isolates were grown in liquid synthetic medium supplemented with glucose, pectin, carboxymethylcellulose or xylan as carbon sources and/or enzyme inducers and glutamic acid as nitrogen source. Pectinases were the first cell wall-degrading enzymes detected and the activities obtained (polygalacturonase activity was between 0.4 and 1.3 U/ml and polymethylgalacturonase between 0.15 and 1.3 U/ml) were higher than those of cellulases and xylanases, which appeared later and in a lesser magnitude. This sequence would promote initial tissue maceration followed by cell wall degradation. Laccase was detected in all the isolates evaluated (activity was between 36 U/l and 63 U/l). The aggressiveness of the isolates was tested in maize, sunflower and watermelon seeds, being high on all the plants assayed. This study reports for the first time the potential of different isolates of M. phaseolina to produce plant cell wall-degrading enzymes in submerged fermentation.

Macrophomina phaseolina es un fitopatógeno polífago, causante de podredumbre carbonosa. Los daños que genera en cultivos de soja y maíz bajo siembra directa en Argentina, en períodos secos y calurosos, se incrementaron por su habilidad para sobrevivir como esclerocios en suelos y restos de cosecha. El propósito del trabajo fue estudiar la producción in vitro de enzimas degradadoras de pared celular vegetal (pectinasas --#91;poligalacturonasa y polimetilgalacturonasa--#93;; celulasas --#91;endoglucanasa--#93;; hemicelulasas --#91;endoxilanasa--#93; y la enzima ligninolítica lacasa) de varios aislamientos argentinos de M. phaseolina y evaluar la patogenicidad de esos aislamientos, como paso preliminar para establecer el papel de estas enzimas en la interacción M. phaseolina-maíz. Se estudió la cinética de crecimiento del hongo y la de la producción de dichas enzimas en medios de cultivo líquidos sintéticos con ácido glutámico como fuente de nitrógeno y con pectina, carboximetilcelulosa (CMC) o xilano como fuentes de carbono. Las pectinasas fueron las primeras enzimas detectadas y los máximos títulos registrados (1,4 UE/ml --#91;poligalacturonasa--#93; y 1,2 UE/ml --#91;polimetilgalacturonasa--#93;, respectivamente) superaron a los de celulasas y xilanasas, que aparecieron más tardíamente y en menor magnitud. Esta secuencia promovería la maceración inicial del tejido, seguida luego por la degradación de la pared celular vegetal. Se detectó actividad lacasa en todos los aislamientos (36 a 63 U/l). La agresividad de todos los aislamientos resultó alta en los 3 hospedantes evaluados: semillas de maíz, de girasol y de melón. En este trabajo se investiga por primera vez el potencial de distintos aislamientos de M. phaseolina para producir enzimas degradadoras de pared celular vegetal en cultivo líquido.
Descritores: Técnicas In Vitro/métodos
Parede Celular/enzimologia
Zea mays/enzimologia
Zea mays/parasitologia
-Poligalacturonase/isolamento & purificação
Celulase/isolamento & purificação
Endo-1,4-beta-Xilanases/isolamento & purificação
Tipo de Publ: Ensaio Clínico
Estudo de Avaliação
Estudo Observacional
Responsável: AR635.1 - FCVyS - Servicio de Información y Documentación


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Id: biblio-1254696
Autor: Li, Feng; Xie, Yingjie; Gao, Xiang; Shan, Mingxu; Sun, Changchao; Dong Niu, Yan; Shan, Anshan.
Título: Screening of cellulose degradation bacteria from Min pigs and optimization of its cellulase production
Fonte: Electron. j. biotechnol;48:29-35, nov. 2020. ilus, tab, graf.
Idioma: en.
Projeto: Open Project of Northeastern Science Inspection Station and China Ministry of Agriculture Key; . Laboratory of Animal Pathogen Biology; . China Agriculture Research System.
Resumo: BACKGROUND: Cellulose as a potential feed resource hinders its utilization because of its complex structure, and cellulase is the key to its biological effective utilization. Animal endogenous probiotics are more susceptible to colonization in the intestinal tract, and their digestive enzymes are more conducive to the digestion and absorption of feed in young animals. Min pigs are potential sources of cellulase probiotics because of the high proportion of dietary fiber in their feed. In this study, the cellulolytic bacteria in the feces of Min pigs were isolated and screened. The characteristics of enzymes and cellulase production were studied, which provided a theoretical basis for the rational utilization of cellulase and high-fiber food in animal production. RESULTS: In our study, 10 strains of cellulase producing strains were isolated from Min pig manure, among which the M2 strain had the best enzyme producing ability and was identified as Bacillus velezensis. The optimum production conditions of cellulase from strain M2 were: 2% inoculum, the temperature of 35°C, the pH of 5.0, and the liquid loading volume of 50 mL. The optimum temperature, pH and time for the reaction of cellulase produced by strain M2 were 55°C, 4.5 and 5 min, respectively. CONCLUSIONS: Min pigs can be used as a source of cellulase producing strains. The M2 strain isolated from feces was identified as Bacillus velezensis. The cellulase from M2 strain had a good activity and the potential to be used as feed additive for piglets.
Descritores: Porco Miniatura
Bactérias/enzimologia
Celulase/biossíntese
-Bacillus
Fibras na Dieta
Probióticos
Digestão
Fezes
Ração Animal
Limites: Animais
Responsável: CL1.1 - Biblioteca Central


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Id: biblio-1132193
Autor: Dudek, Débora Nakadomari; Bueno, Indianara Kawana; Rasbold, Leticia Mara; Pagnonceli, Juliana; Corrêa, Juliana Moço; Silva, José Luis da Conceição; Kadowaki, Marina Kimiko; Simão, Rita de Cássia Garcia; Silva, Roberto Nascimento; Maller, Alexandre.
Título: Enhance of Cellulase Production and Biomass Degradation by Transformation of the Trichoderma reesei RUT-C30∆ zface1 Strain
Fonte: Braz. arch. biol. technol;63:e20190185, 2020. tab, graf.
Idioma: en.
Projeto: National Council for Scientific and Technological Development.
Resumo: Abstract The second-generation bioethanol employs lignocellulosic materials degraded by microbial cellulases in their production. The fungus Trichoderma reesei is one of the main microorganisms producing cellulases, and its genetic modification can lead to the optimization in obtaining hydrolytic enzymes. This work carried out the deletion of the sequence that encodes the zinc finger motif of the transcription factor ACE1 (cellulase expression repressor I) of the fungus T. reesei RUT-C30. The transformation of the RUT-C30 lineage was confirmed by amplification of the 989 bp fragment relative to the selection marker, and by the absence of the zinc finger region amplification in mutants, named T. reesei RUT-C30Δzface1. The production of cellulases by mutants was compared to RUT-C30 and measured with substrates carboxymethylcellulose (CMC), microcrystalline cellulose (Avicel®) and Whatman filter paper (PF). The results demonstrated that RUT-C30Δzface1 has cellulolytic activity increased 3.2-fold in Avicel and 2.1-fold in CMC and PF. The mutants presented 1.4-fold higher sugar released in the hydrolysis of the biomass assays. These results suggest that the partial deletion of ace1 gene is an important strategy in achieving bioethanol production on an industrial scale at a competitive price in the fuel market.
Descritores: Trichoderma/enzimologia
Celulase/biossíntese
Dedos de Zinco
Biomassa
Etanol
Biocombustíveis
Responsável: BR1.1 - BIREME


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Id: biblio-1146380
Autor: BELMONT-MONTEFUSCO, Enide Luciana; NACIF-MARÇAL, Lorena; ASSUNÇÃO, Enedina Nogueira de; HAMADA, Neusa; NUNES-SILVA, Carlos Gustavo.
Título: Cultivable cellulolytic fungi isolated from the gut of Amazonian aquatic insects
Fonte: Acta amaz;50(4):346-354, out. - dez. 2020.
Idioma: en.
Resumo: Fungos filamentosos tem sido alvo de estudos de bioprospecção devido à sua grande eficiencia em produzir enzimas extracelulares, as quais tem grande potencial para uso em bioindústrias. Neste estudo, fungos filamentosos foram isolados do intestino de larvas de insetos aquáticos da Amazônia, para avaliar sua atividade celulolítica. Foram coletadas 69 larvas de insetos aquáticos fragmentadores de três gêneros: Phylloicus (Trichoptera: Calamoceratidae), Triplectides (Trichoptera:Leptoceridae) e Stenochironomus (Diptera: Chironomidae) em dez igarapés de uma área protegida na Amazônia central brasileira. O crescimento dos fungos isolados foi feito em meio de cultura Ágar Batata Dextrose (BDA). Os isolados fúngicos foram transferidos para o meio sintético com Carboximetil celulose e utilizou-se vermelho Congo para determinar o índice enzimático. O halo de hidrólise, indicando a produção de celulases, foi observado em 175 isolados fúngicos (70% do total), dos quais 25 tiveram um índice enzimático ≥ 2,0 e pertencem a sete gêneros fúngicos. Os táxons fúngicos Cladosporium, Gliocephalotrichum, Penicillium, Pestalotiopsis, Talaromyces, Trichoderma e Umbelopsis foram isolados dos intestinos das larvas de Phylloicus, Triplectides e Stenochironomus e são tradicionalmente utilizados em aplicações biotecnológicas. Os resultados indicam um potencial celulolítco destes fungos associados ao intestino de insetos aquáticos amazônicos. (AU)
Descritores: Celulase
Fragmentadores
Ecossistema Amazônico
Hidrólise
Responsável: BR6.1 - BCS - Biblioteca de Ciências da Saúde


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Id: biblio-886801
Autor: MARCO, ÉVILIN G DE; HECK, KARINA; MARTOS, EMERSON T; VAN DER SAND, SUELI T.
Título: Purification and characterization of a thermostable alkaline cellulase produced by Bacillus licheniformis 380 isolated from compost
Fonte: An. acad. bras. ciênc;89(3,supl):2359-2370, 2017. tab, graf.
Idioma: en.
Resumo: ABSTRACT During composting processes, the degradation of organic waste is accomplished and driven by a succession of microbial populations exhibiting a broad range of functional competencies. A total of 183 bacteria, isolated from a composting process, were evaluated for cellulase activity at different temperatures (37, 50, 60, and 70°C) and pH values. Out of the 22 isolates that showed activity, isolate 380 showed the highest cellulase activity. Its ability to produce cellulase was evaluated in culture medium supplemented with carboxymethyl cellulose, microcrystalline cellulose, wheat straw, and rice husk. The culture medium supplemented with carboxymethyl cellulose induced higher enzyme activity after 6 hours of incubation (0.12 UEA mL-1 min-1). For wheat straw and rice husk, the results were 0.08 UEA mL-1 min-1 for both, while for microcrystalline cellulose, 0.04 UEA mL-1 min-1 were observed. The highest carboxymethyl cellulase activity was observed at 60°C (0.14 UEA mL-1 min-1) for both crude and partially purified enzyme after 30 and 120 min of incubation, respectively. Alkalinization of the medium was observed during cultivation in all substrates. The cellulase had a molecular mass of 20 kDa determined by SDS-Page. Isolate 380 was identified as Bacillus licheniformis. This work provides a basis for further studies on composting optimization.
Descritores: Carboximetilcelulose Sódica/farmacologia
Celulase/isolamento & purificação
Celulase/biossíntese
Meios de Cultura/farmacologia
Bacillus licheniformis/enzimologia
-Especificidade por Substrato
Eletroforese em Gel de Poliacrilamida
Bacillus licheniformis/efeitos dos fármacos
Temperatura Alta
Responsável: BR1.1 - BIREME


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Id: biblio-951810
Autor: Rungrattanakasin, Budsayachat; Premjet, Siripong; Thanonkeo, Sudarat; Klanrit, Preekamol; Thanonkeo, Pornthap.
Título: Cloning and expression of an endoglucanase gene from the thermotolerant fungus Aspergillus fumigatus DBiNU-1 in Kluyveromyces lactis
Fonte: Braz. j. microbiol;49(3):647-655, July-Sept. 2018. graf.
Idioma: en.
Resumo: Abstract An intronless endoglucanase from thermotolerant Aspergillus fumigatus DBINU-1 was cloned, characterized and expressed in the yeast Kluyveromyces lactis. The full-length open reading frame of the endoglucanase gene from A. fumigatus DBiNU-1, designated Cel7, was 1383 nucleotides in length and encoded a protein of 460 amino acid residues. The predicted molecular weight and the isoelectric point of the A. fumigatus Cel7 gene product were 48.19 kDa and 5.03, respectively. A catalytic domain in the N-terminal region and a fungal type cellulose-binding domain/module in the C-terminal region were detected in the predicted polypeptide sequences. Furthermore, a signal peptide with 20 amino acid residues at the N-terminus was also detected in the deduced amino acid sequences of the endoglucanase from A. fumigatus DBiNU-1. The endoglucanase from A. fumigatus DBiNU-1 was successfully expressed in K. lactis, and the purified recombinant enzyme exhibited its maximum activity at pH 5.0 and 60 °C. The enzyme was very stable in a pH range from 4.0 to 8.0 and a temperature range from 30 to 60 °C. These features make it suitable for application in the paper, biofuel, and other chemical production industries that use cellulosic materials.
Descritores: Aspergillus fumigatus/enzimologia
Proteínas Fúngicas/genética
Proteínas Fúngicas/química
Expressão Gênica
Celulase/genética
Celulase/química
Clonagem Molecular
-Aspergillus fumigatus/genética
Especificidade por Substrato
Estabilidade Enzimática
Kluyveromyces/genética
Kluyveromyces/metabolismo
Proteínas Recombinantes/genética
Proteínas Recombinantes/metabolismo
Proteínas Recombinantes/química
Proteínas Fúngicas/metabolismo
Celulase/metabolismo
Temperatura Alta
Concentração de Íons de Hidrogênio
Responsável: BR1.1 - BIREME


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Id: lil-780841
Autor: Bottino, Flávia; Cunha-Santino, Marcela Bianchessi; Bianchini Jr, Irineu.
Título: Cellulase activity and dissolved organic carbon release from lignocellulose macrophyte-derived in four trophic conditions
Fonte: Braz. j. microbiol;47(2):352-358, Apr.-June 2016. tab, graf.
Idioma: en.
Projeto: São Paulo Research Foundation.
Resumo: Abstract Considering the importance of lignocellulose macrophyte-derived for the energy flux in aquatic ecosystems and the nutrient concentrations as a function of force which influences the decomposition process, this study aims to relate the enzymatic activity and lignocellulose hydrolysis in different trophic statuses. Water samples and two macrophyte species were collected from the littoral zone of a subtropical Brazilian Reservoir. A lignocellulosic matrix was obtained using aqueous extraction of dried plant material (≈40 °C). Incubations for decomposition of the lignocellulosic matrix were prepared using lignocelluloses, inoculums and filtered water simulating different trophic statuses with the same N:P ratio. The particulate organic carbon and dissolved organic carbon (POC and DOC, respectively) were quantified, the cellulase enzymatic activity was measured by releasing reducing sugars and immobilized carbon was analyzed by filtration. During the cellulose degradation indicated by the cellulase activity, the dissolved organic carbon daily rate and enzyme activity increased. It was related to a fast hydrolysable fraction of cellulose that contributed to short-term carbon immobilization (ca. 10 days). After approximately 20 days, the dissolved organic carbon and enzyme activity were inversely correlated suggesting that the respiration of microorganisms was responsible for carbon mineralization. Cellulose was an important resource in low nutrient conditions (oligotrophic). However, the detritus quality played a major role in the lignocelluloses degradation (i.e., enzyme activity) and carbon release.
Descritores: Bactérias/enzimologia
Proteínas de Bactérias/metabolismo
Celulase/metabolismo
Araceae/metabolismo
Paspalum/metabolismo
Água Doce/química
Lignina/metabolismo
-Brasil
Carbono/metabolismo
Celulose/genética
Celulose/metabolismo
Ecossistema
Araceae/crescimento & desenvolvimento
Araceae/microbiologia
Paspalum/crescimento & desenvolvimento
Paspalum/microbiologia
Água Doce/microbiologia
Responsável: BR1.1 - BIREME


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Id: biblio-1048705
Autor: Felber, Aretusa Cristina; Specian, Vânia; Orlandelli, Ravely Casarotti; Costa, Alessandra Tenório; Polonio, Julio Cesar; Mourão, Káthia Socorro Mathias; Pamphile, João Alencar.
Título: Endoglucanase production by endophytic fungi isolated from vitis labrusca l. with peanut hull and sawdust as substrates / Produção de endoglucanase por fungos endofíticos isolados de Vitis labrusca L. utilizando casca de amendoim e serragem como substratos
Fonte: Biosci. j. (Online);35(3):933-940, may./jun. 2019. tab.
Idioma: en.
Resumo: Endoglucanases are enzymes widely employed in different industrial fields, albeit with high production costs. Studies on new microbial sources and low-cost substrates are highly relevant, including those on agro-industrial. Current analysis evaluates peanut hull (PH) and sawdust (SD) as substrates for submerged cultures of 14 endophytic fungi isolated from grapevine (Vitis labrusca L.) cultivars Bordô and Concord. Endophytes were grown on a carboxymethylcellulose (CMC) medium and the cup plate assay showed that eight strains (belonging to genera Cochliobolus, Diaporthe, Fusarium and Phoma) had positive results: enzymatic halos ranged from 10.8±0.02to 15.5±0.07 mm in diameter. Diaporthe sp. strains (GenBank accession codes KM362392, KM362368 and KM362378) and Fusariumculmorum KM362384 were highlighted as the most promising sources. Further, PH and SD as substrates for the fermentation of these fungi were evaluated by the cup plate assay and endoglucanase activity assay. Highest halo diameters were obtained for Diaporthe sp. KM362392: 16.1±0.01 mm (CMC), 14.5±0.01 mm (PH) and 14.7±0.03 mm (SD). The fungus also presented the highest levels of endoglucanase activity: analysis of variance revealed that CMC (3.52±0.98 µmol/min), PH (2.93±0.23 µmol/min) and SD (3.26±0.38 µmol/min) were similarly efficient as substrates. Results deepen knowledge on V. labrusca endophytes that may be endoglucanase sources, eventhough further optimizations in submerged cultures with PH and SD should be undertaken to increase theenzymatic production from these wastes.

Endoglucanases são enzimas amplamente empregadas em diferentes setores industriais; embora sua produção apresente custos elevados. Estudos sobre novas fontes microbianas e substratos mais baratos são de grande importância, incluindo os resíduos agroindustriais. Nesse estudo, casca de amendoim (CA) e serragem (SE) foram testadas como substratos para o cultivo submerso de 14 fungos endofíticos isolados das cultivares Bordô e Concord de videira (Vitis labrusca L.) Os endófitos foram crescidos em meio contendo carboximetilcelulose (CMC) e o ensaio cup plate mostrou resultados positivos para oito fungos (pertencentes aos gêneros Cochliobolus, Diaporthe, Fusarium and Phoma); os halos enzimáticos variaram entre 10,8±0,02 e 15,5±0,07 mm de diâmetro. Linhagens de Diaporthe sp. (códigos de acesso no GenBank KM362392, KM362368 e KM362378) e Fusariumculmorum KM362384 se destacaram como produtores mais promissores. Então, o uso de CA e SE como substratos para a fermentação desses fungos foi avaliado pelo ensaio cup plate e pela quantificação da atividade de endoglucanase. Os maiores halos enzimáticos foram obtidos para Diaporthe sp. KM362392: 16,1±0,01 mm (CMC), 14,5±0,01 mm (CA) e 14,7±0,03 mm (SE). Esse fungo também apresentou os maiores níveis de endoglucanase: a análise de variância revelou que CMC (3,52±0,98 µmol/min), CA (2,93±0,23 µmol/min) e SE (3,26±0,38 µmol/min) foram substratos similarmente eficientes. Esses resultados expandem o conhecimento sobre endófitos de V. labrusca que são fontes de endoglucanases; futuras otimizações quanto ao cultivo submerso com CA e SE podem ser utilizadas para aumentar a produção enzimática a partir do uso desses resíduos.
Descritores: Resíduos
Celulase
Substratos para Tratamento Biológico
Enzimas
Agroindústria
Endófitos
Responsável: BR396.1 - Biblioteca Central


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ASTOLFI-FILHO, SPARTACO
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Id: biblio-1022139
Autor: Santa-Rosa, Pamella S; Souza, Anita L; Roque, Rosemary A; Andrade, Edmar V; Astolfi-Filho, Spartaco; Mota, Adolfo J; Nunes-Silva, Carlos G.
Título: Production of thermostable ß-glucosidase and CMCase by Penicillium sp: LMI01 isolated from the Amazon region
Fonte: Electron. j. biotechnol;31:84-92, Jan. 2018. graf, tab, ilus.
Idioma: en.
Projeto: Fundação de Amparo a Pesquisa do Amazonas, FAPEAM - PAPPE Integração; . Coordenação de Aperfeiçoamento de Pessoal Nível Superior, CAPES; . Conselho Nacional de Desenvolvimento Científico e Tecnológico, CNPq.
Resumo: Background: Cellulolytic enzymes of microbial origin have great industrial importance because of their wide application in various industrial sectors. Fungi are considered the most efficient producers of these enzymes. Bioprospecting survey to identify fungal sources of biomass-hydrolyzing enzymes from a high-diversity environment is an important approach to discover interesting strains for bioprocess uses. In this study, we evaluated the production of endoglucanase (CMCase) and ß-glucosidase, enzymes from the lignocellulolytic complex, produced by a native fungus. Penicillium sp. LMI01 was isolated from decaying plant material in the Amazon region, and its performance was compared with that of the standard isolate Trichoderma reesei QM9414 under submerged fermentation conditions. Results: The effectiveness of LMI01 was similar to that of QM9414 in volumetric enzyme activity (U/mL); however, the specific enzyme activity (U/mg) of the former was higher, corresponding to 24.170 U/mg of CMCase and 1.345 U/mg of ß-glucosidase. The enzymes produced by LMI01 had the following physicochemical properties: CMCase activity was optimal at pH 4.2 and the ß-glucosidase activity was optimal at pH 6.0. Both CMCase and ß-glucosidase had an optimum temperature at 60°C and were thermostable between 50 and 60°C. The electrophoretic profile of the proteins secreted by LMI01 indicated that this isolate produced at least two enzymes with CMCase activity, with approximate molecular masses of 50 and 35 kDa, and ß-glucosidases with molecular masses between 70 and 100 kDa. Conclusions: The effectiveness and characteristics of these enzymes indicate that LMI01 can be an alternative for the hydrolysis of lignocellulosic materials and should be tested in commercial formulations.
Descritores: Penicillium/enzimologia
Celulase/biossíntese
beta-Glucosidase/biossíntese
-Oligossacarídeos
Temperatura
Trichoderma/enzimologia
Estabilidade Enzimática
Celulase/metabolismo
beta-Glucosidase/metabolismo
Ecossistema Amazônico
Biocatálise
Fermentação
Concentração de Íons de Hidrogênio
Hidrólise
Lignina/metabolismo
Responsável: CL1.1 - Biblioteca Central



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