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Pesquisa : D08.811.277.450.420.200.200 [Categoria DeCS]
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Id: biblio-889226
Autor: Thomas, Lebin; Ram, Hari; Singh, Ved Pal.
Título: Inducible cellulase production from an organic solvent tolerant Bacillus sp. SV1 and evolutionary divergence of endoglucanase in different species of the genus Bacillus
Fonte: Braz. j. microbiol;49(2):429-442, Apr.-June 2018. tab, graf.
Idioma: en.
Resumo: Abstract Bacteria are important sources of cellulases with various industrial and biotechnological applications. In view of this, a non-hemolytic bacterial strain, tolerant to various environmental pollutants (heavy metals and organic solvents), showing high cellulolytic index (7.89) was isolated from cattle shed soil and identified as Bacillus sp. SV1 (99.27% pairwise similarity with Bacillus korlensis). Extracellular cellulases showed the presence of endoglucanase, total cellulase and β-glucosidase activities. Cellulase production was induced in presence of cellulose (3.3 times CMCase, 2.9 times FPase and 2.1 times β-glucosidase), and enhanced (115.1% CMCase) by low-cost corn steep solids. An in silico investigation of endoglucanase (EC 3.2.1.4) protein sequences of three Bacillus spp. as query, revealed their similarities with members of nine bacterial phyla and to Eukaryota (represented by Arthropoda and Nematoda), and also highlighted of a convergent and divergent evolution from other enzymes of different substrate [(1,3)-linked beta-d-glucans, xylan and chitosan] specificities. Characteristic conserved signature indels were observed among members of Actinobacteria (7 aa insert) and Firmicutes (9 aa insert) that served as a potential tool in support of their relatedness in phylogenetic trees.
Descritores: Bacillus/enzimologia
Celulase/genética
Celulase/metabolismo
Evolução Molecular
-Bacillus/crescimento & desenvolvimento
Bacillus/isolamento & purificação
Celulose/metabolismo
Biologia Computacional
Fezes/microbiologia
Regulação Bacteriana da Expressão Gênica
Regulação Enzimológica da Expressão Gênica
Mutação INDEL
Análise de Sequência de DNA
Homologia de Sequência
Especificidade por Substrato
Zea mays/metabolismo
Limites: Animais
Bovinos
Responsável: BR1.1 - BIREME


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Id: biblio-889172
Autor: Song, Yun-Hee; Lee, Kyung-Tai; Baek, Jin-Young; Kim, Min-Ju; Kwon, Mi-Ra; Kim, Young-Joo; Park, Mi-Rim; Ko, Haesu; Lee, Jin-Sung; Kim, Keun-Sung.
Título: Isolation and characterization of a novel endo-beta-1, 4-glucanase from a metagenomic library of the black-goat rumen
Fonte: Braz. j. microbiol;48(4):801-808, Oct.-Dec. 2017. tab, graf.
Idioma: en.
Resumo: ABSTRACT The various types of lignocellulosic biomass found in plants comprise the most abundant renewable bioresources on Earth. In this study, the ruminal microbial ecosystem of black goats was explored because of their strong ability to digest lignocellulosic forage. A metagenomic fosmid library containing 115,200 clones was prepared from the black-goat rumen and screened for a novel cellulolytic enzyme. The KG35 gene, containing a novel glycosyl hydrolase family 5 cellulase domain, was isolated and functionally characterized. The novel glycosyl hydrolase family 5 cellulase gene is composed of a 963-bp open reading frame encoding a protein of 320 amino acid residues (35.1 kDa). The deduced amino acid sequence showed the highest sequence identity (58%) for sequences from the glycosyl hydrolase family 5 cellulases. The novel glycosyl hydrolase family 5 cellulase gene was overexpressed in Escherichia coli. Substrate specificity analysis revealed that this recombinant glycosyl hydrolase family 5 cellulase functions as an endo-β-1,4-glucanase. The recombinant KG35 endo-β-1,4-glucanase showed optimal activity within the range of 30-50 °C at a pH of 6-7. The thermostability was retained and the pH was stable in the range of 30-50 °C at a pH of 5-7.
Descritores: Proteínas de Bactérias/química
Proteínas de Bactérias/genética
Bactérias/enzimologia
Celulase/química
Celulase/genética
Rúmen/microbiologia
-Proteínas de Bactérias/metabolismo
Bactérias/classificação
Bactérias/genética
Bactérias/isolamento & purificação
Celulase/metabolismo
Clonagem Molecular
Estabilidade Enzimática
Microbioma Gastrointestinal
Cabras
Concentração de Íons de Hidrogênio
Metagenoma
Metagenômica
Limites: Animais
Responsável: BR1.1 - BIREME


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Id: lil-741270
Autor: Avellaneda-Torres, Lizeth Manuela; Pulido, Claudia Patricia Guevara; Rojas, Esperanza Torres.
Título: Assessment of cellulolytic microorganisms in soils of Nevados Park, Colombia
Fonte: Braz. j. microbiol;45(4):1211-1220, Oct.-Dec. 2014. graf, mapas, tab.
Idioma: en.
Projeto: Colciencias; . MAVDT; . UAESPNN.
Resumo: A systematized survey was conducted to find soil-borne microbes that degrade cellulose in soils from unique ecosystems, such as the Superpáramo, Páramo, and the High Andean Forest in the Nevados National Natural Park (NNNP), Colombia. These high mountain ecosystems represent extreme environments, such as high levels of solar radiation, low atmospheric pressure, and extreme daily changes in temperature. Cellulolytic activity of the microorganisms was evaluated using qualitative tests, such as growth in selective media followed by staining with congo red and iodine, and quantitative tests to determine the activity of endoglucanase, β-glucosidase, exoglucanase, and total cellulase. Microorganisms were identified using molecular markers, such as the 16S rRNA gene for bacteria and the internal transcribed spacer region (ITS) of ribosomal DNA for fungi. Multivariate statistical analysis (MVA) was used to select microorganisms with high cellulolytic capacity. A total of 108 microorganisms were isolated from the soils and, in general, the enzymatic activities of fungi were higher than those of bacteria. Our results also found that none of the organisms studied were able to degrade all the components of the cellulose and it is therefore suggested that a combination of bacteria and/or fungi with various enzymatic activities be used to obtain high total cellulolytic activity. This study gives an overview of the potential microorganism that could be used for cellulose degradation in various biotechnological applications and for sustainable agricultural waste treatment.
Descritores: Bactérias/isolamento & purificação
Bactérias/metabolismo
Celulose/metabolismo
Fungos/isolamento & purificação
Fungos/metabolismo
Microbiologia do Solo
-Bactérias/classificação
Bactérias/genética
Colômbia
Celulase/análise
DNA Bacteriano/química
DNA Bacteriano/genética
DNA Fúngico/química
DNA Fúngico/genética
DNA de Helmintos/química
DNA de Helmintos/genética
DNA Espaçador Ribossômico/química
DNA Espaçador Ribossômico/genética
DNA Ribossômico/química
DNA Ribossômico/genética
Fungos/classificação
Fungos/genética
Hidrólise
/genética
RNA, RIBOSOMAL, ABNORMALITIES, MULTIPLES/genética
Análise de Sequência de DNA
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


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Id: lil-723113
Autor: El-Naggar, Noura El-Ahmady; Abdelwahed, Nayera A.M.; Saber, Wesam I.A.; Mohamed, Asem A..
Título: Bioprocessing of some agro-industrial residues for endoglucanase production by the new subsp.; Streptomyces albogriseolus subsp. cellulolyticus strain NEAE-J
Fonte: Braz. j. microbiol;45(2):743-751, Apr.-June 2014. ilus, tab.
Idioma: en.
Resumo: The use of low cost agro-industrial residues for the production of industrial enzymes is one of the ways to reduce significantly production costs. Cellulase producing actinomycetes were isolated from soil and decayed agricultural wastes. Among them, a potential culture, strain NEAE-J, was selected and identified on the basis of morphological, cultural, physiological and chemotaxonomic properties, together with 16S rDNA sequence. It is proposed that strain NEAE-J should be included in the species Streptomyces albogriseolus as a representative of a novel sub-species, Streptomyces albogriseolus subsp. cellulolyticus strain NEAE-J and sequencing product was deposited in the GenBank database under accession number JN229412. This organism was tested for its ability to produce endoglucanase and release reducing sugars from agro-industrial residues as substrates. Sugarcane bagasse was the most suitable substrate for endoglucanase production. Effects of process variables, namely incubation time, temperature, initial pH and nitrogen source on production of endoglucanase by submerged fermentation using Streptomyces albogriseolus subsp. cellulolyticus have been studied. Accordingly optimum conditions have been determined. Incubation temperature of 30 ºC after 6 days, pH of 6.5, 1% sugarcane bagasse as carbon source and peptone as nitrogen source were found to be the optimum for endoglucanase production. Optimization of the process parameters resulted in about 2.6 fold increase in the endoglucanase activity. Therefore, Streptomyces albogriseolus subsp. cellulolyticus coud be potential microorganism for the intended application.
Descritores: Celulase/isolamento & purificação
Celulase/metabolismo
Streptomyces/metabolismo
-Técnicas de Tipagem Bacteriana
Análise por Conglomerados
Carboidratos/análise
Celulose/metabolismo
Meios de Cultura/química
DNA Bacteriano/química
DNA Bacteriano/genética
DNA Ribossômico/química
DNA Ribossômico/genética
Concentração de Íons de Hidrogênio
Dados de Sequência Molecular
Filogenia
/genética
RNA, RIBOSOMAL, ABNORMALITIES, MULTIPLES/genética
Análise de Sequência de DNA
Saccharum/metabolismo
Streptomyces/classificação
Streptomyces/crescimento & desenvolvimento
Streptomyces/isolamento & purificação
Temperatura Ambiente
Fatores de Tempo
Responsável: BR1.1 - BIREME


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Id: lil-709463
Autor: Kilikian, B.V.; Afonso, L.C.; Souza, T.F.C.; Ferreira, R.G.; Pinheiro, I.R..
Título: Filamentous fungi and media for cellulase production in solid state cultures
Fonte: Braz. j. microbiol;45(1):279-286, 2014. graf, tab.
Idioma: en.
Resumo: Cellulase production was evaluated in two reference strains (T. reesei Rut-C30 and T. reesei QM9414), two strains isolated from a sugarcane cultivation area (Trichoderma sp. IPT778 and T. harzianum rifai IPT821) and one strain isolated in a program for biodiversity preservation in São Paulo state (Myceliophthora thermophila M77). Solid state cultures were performed using sugarcane bagasse (C), wheat bran (W) and/or soybean bran (S). The highest FPA was 10.6 U/gdm for M77 in SC (10:90) at 80% moisture, which was 4.4 times higher than production in pure W. C was a strong inducer of cellulase production, given that the production level of 6.1 U/gdm in WC (40:60) was 2.5 times higher than in pure W for strain M77; T. reesei Rut-C30 did not respond as strongly with about 1.6-fold surplus production. S advantageously replaced W, as the surplus production on SC (20:80) was 2.3 times relative to WC (20:80) for M77.
Descritores: Biotecnologia/métodos
Celulase/metabolismo
Meios de Cultura/química
Fungos/enzimologia
Fungos/crescimento & desenvolvimento
-Fibras na Dieta/metabolismo
Saccharum/metabolismo
Sordariales/enzimologia
Sordariales/crescimento & desenvolvimento
Feijão de Soja/metabolismo
Trichoderma/enzimologia
Trichoderma/crescimento & desenvolvimento
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


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Id: lil-676918
Autor: Belal, Elsayed B.
Título: Bioethanol production from rice straw residues
Fonte: Braz. j. microbiol;44(1):225-234, 2013. ilus, tab.
Idioma: en.
Resumo: A rice straw -cellulose utilizing mold was isolated from rotted rice straw residues. The efficient rice straw degrading microorganism was identified as Trichoderma reesei. The results showed that different carbon sources in liquid culture such as rice straw, carboxymethyl cellulose, filter paper, sugar cane bagasse, cotton stalk and banana stalk induced T. reesei cellulase production whereas glucose or Potato Dextrose repressed the synthesis of cellulase. T. reesei cellulase was produced by the solid state culture on rice straw medium. The optimal pH and temperature for T. reesei cellulase production were 6 and 25 ºC, respectively. Rice straw exhibited different susceptibilities towards cellulase to their conversion to reducing sugars. The present study showed also that, the general trend of rice straw bioconversion with cellulase was more than the general trend by T. reesei. This enzyme effectively led to enzymatic conversion of acid, alkali and ultrasonic pretreated cellulose from rice straw into glucose, followed by fermentation into ethanol. The combined method of acid pretreatment with ultrasound and subsequent enzyme treatment resulted the highest conversion of lignocellulose in rice straw to sugar and consequently, highest ethanol concentration after 7 days fermentation with S. cerevisae yeast. The ethanol yield in this study was about 10 and 11 g.L-¹.
Descritores: Biomassa
Carbono
Celulase/análise
Celulase/isolamento & purificação
Etanol/análise
Microbiologia Industrial
Lixo
Oryza/enzimologia
Trichoderma/enzimologia
Trichoderma/isolamento & purificação
-Hidrólise
Métodos
Técnicas
Tipo de Publ: Estudo Comparativo
Responsável: BR32.1 - Serviço de Biblioteca e Informação Biomédica


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Id: lil-657677
Autor: Odisi, Estácio Jussie; Silvestrin, Marcela Bruschi; Takahashi, Rodrigo Yoji Uwamori; da Silva, Marcus Adonai Castro; Lima, André Oliveira de Souza.
Título: Bioprospection of cellulolytic and lipolytic South Atlantic deep-sea bacteria
Fonte: Electron. j. biotechnol;15(5):18-18, Sept. 2012. ilus, tab.
Idioma: en.
Projeto: International Centre for Genetic Engineering and Biotechnology; . Brazilian National Council for Scientific and Technological Development.
Resumo: Background: Cellulases and lipases have broad industrial application, which calls for an urgent exploration of microorganisms from extreme environments as valuable source of commercial enzyme. In this context, the present work describes the bioprospection and identification of deep-sea bacteria that produce cellulases and lipases, as well their optimal temperature of activity. Results: The first step of this study was the screening of cellulolytic and lipolytic deep-sea bacteria from sediment and water column, which was conducted with substrates linked with 4-Methylumbelliferyl. Among the 161 strains evaluated, 40 were cellulolytic, 23 were lipolytic and 5 exhibited both activities. Cellulolytic and lipolytic bacteria are more common in sediment than at the water column. Based on the ability to produce cellulases and lipases three isolates were selected and identified (16S rRNA sequencing) as Bacillus stratosphericus, B. aerophilus and B. pumilus. Lipases of strain B. aerophilus LAMA 582 exhibited activity at a wide temperature range (4º to 37ºC) and include psychrophilic behaviour. Strain Bacillus stratosphericus LAMA 585 can growth in a rich (Luria Bertani) and minimal (Marine Minimal) medium, and does not need an inducer to produce its mesophilic cellulases and lipases. Conclusions: Deep-sea sediments have great potential for bioprospection of cellulase and lipase-producing bacteria. The strains LAMA 582 and LAMA 585 with their special features, exhibit a great potential to application at many biotechnology process.
Descritores: Água do Mar/microbiologia
Bactérias/enzimologia
Celulase
Lipase
-Bioprospecção
Responsável: CL1.1 - Biblioteca Central


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Id: lil-657620
Autor: Levin, Laura; Diorio, Luis; Grassi, Emanuel; Forchiassin, Flavia.
Título: Grape stalks as substrate for white rot fungi, lignocellulolytic enzyme production and dye decolorization / Uso del escobajo como sustrato para el crecimiento de hongos de la pudrición blanca, la producción de enzimas ligninolíticas y la decoloración de tinturas
Fonte: Rev. argent. microbiol;44(2):105-112, jun. 2012. graf, tab.
Idioma: es.
Resumo: The aim of this work was to evaluate the potential of grape stalks, an agroindustrial waste, for growth and lignocellulolytic enzyme production via solid-state fermentation, using the following three white rot fungi: Trametes trogii, Stereum hirsutum and Coriolus antarcticus. The decolorization of several dyes by the above mentioned cultures was also investigated. Similar values of dry weight loss of the substrate were measured after 60 days (33-43 %). C. antarcticus produced the highest laccase and Mn-peroxldase activities (33.0 and 1.6 U/g dry solid). The maximum endoglucanase production was measured in S. hirsutum cultures (10.4 U/g), while the endoxylanase peak corresponded to T. trogii (14.6 U/g). The C. antarcticus/grape stalk system seems potentially competitive in bioremediation of textile processing effluents, attaining percentages of decolorization of 93, 86, 82, 82, 77, and 58 % for indigo carmine, malachite green, azure B, remazol brilliant blue R, crystal violet and xylidine, respectively, in 5 h.

El objetivo de este trabajo fue evaluar el potencial del escobajo, un residuo agroindustrial, como sustrato para el crecimiento y la producción de enzimas lignocelulósicas de tres hongos causantes de pudrición blanca en la madera: Trametes trogii, Stereum hirsutum y Coriolus antarcticus. Para ello se utilizaron técnicas de fermentación en estado sólido. También se ensayó la decoloración de colorantes industriales sobre estos cultivos. La pérdida de peso seco del sustrato fue similar después del día 60 (33-43 %). C. antarcticus produjo las mayores actividades de lacasa y Mn-peroxidasa (33,0 y 1,6 U/g peso seco). La mayor actividad endoglucanasa fue medida en cultivos de S. hirsutum (10,4 U/g), y la mayor actividad endoxilanasa en T. trogii (14,6 U/g). El sistema C. antarcticus/escobap mostró un importante potencial para su aplicación en la biorremediación de efluentes textiles, con porcentajes de decoloración de 93, 86, 82, 82, 77 y 58 % para índigo carmín, verde de malaquita, azure B, azul R brillante de remazol, cristal violeta y xilidina, respectivamente, en 5 h.
Descritores: Biodegradação Ambiental
Basidiomycota/crescimento & desenvolvimento
Celulase/isolamento & purificação
Corantes/metabolismo
/isolamento & purificação
ENDO-1,ABBREVIATIONS AS TOPIC-BETA XYLANASES/isolamento & purificação
Proteínas Fúngicas/isolamento & purificação
Resíduos Industriais
Microbiologia Industrial/métodos
Lacase/isolamento & purificação
Lignina/metabolismo
Peroxidases/isolamento & purificação
Caules de Planta/microbiologia
Vitis/microbiologia
-Argentina
Basidiomycota/enzimologia
Celulase/metabolismo
Corantes/classificação
Coriolaceae/enzimologia
Coriolaceae/crescimento & desenvolvimento
/metabolismo
ENDO-1,ABBREVIATIONS AS TOPIC-BETA XYLANASES/metabolismo
Fermentação
Proteínas Fúngicas/metabolismo
Lacase/metabolismo
Peroxidases/metabolismo
Trametes/enzimologia
Trametes/crescimento & desenvolvimento
Tipo de Publ: Estudo Comparativo
Research Support, Non-U.S. Gov't
Responsável: AR1.2 - Instituto de Investigaciónes Epidemiológicas


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Id: lil-656953
Autor: Costa, Marcia; Torres, Marcelo; Reyes, Alejandro.
Título: Caracterización de enzimas hidrolíticas de Aspergillus ficuum producidas en fermentación sólida sobre torta de canola / Characterization of Aspergillus ficuum hydrolytic enzymes produced in solid state fermentation of cold-pressed canola cake
Fonte: Rev. colomb. biotecnol;14(1):208-215, ene.-jun. 2012. ilus, graf, tab.
Idioma: es.
Resumo: En el presente trabajo, se describe la caracterización respecto al pH y temperatura de extractos semipurificados mediante ultrafiltración escalonada de las enzimas fitasa, celulasa y xilanasa de Aspergillus ficuum cepa DSM 932 producidos en fermentación en estado sólido (en adelante SSF) y usando torta de canola como sustrato. La fitasa presentó un pH y una temperatura óptima de 5.0 y 60ºC respectivamente, en tanto que la celulasa presentó un pH óptimo de 7.0 con una temperatura óptima de 60°C y el extracto de xilanasa un pH óptimo de 5.4 y una temperatura óptima de 45°C.(

This paper, describes the characterization respect to pH and temperature of semi-purified extracts by ultrafiltration step of the enzymes phytase, cellulase and xylanase produced by Aspergillus ficuum DSM 932 strain, in solid state fermentation (SSF) using cold-pressed canola cake as substrate. Phytase showed the optimal pH and temperature 6.0 and 60°C, respectively, while cellulase showed a pH optimal of 7.0 with an optimal temperature of 60°C and xylanase extract an optimal pH of 5.4 and an optimal temperature of 45°C.
Descritores: Aspergillus
Celulase
Enzimas
-Ensaios Enzimáticos
Tipo de Publ: Estudo Comparativo
Responsável: CO326 - Departamento de Biología


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Id: lil-656947
Autor: Mateus, Lady; Hernández, Orlando; Velásquez, Mario; Díaz, José de Jesús.
Título: Evaluación del pretratamiento con ácido sulfúrico diluido del pasto maralfalfa (Pennisetum glaucum x Pennisetum purpureum) para la producción de etanol / Dilute sulfuric acid pretreatment of goliath grass (Pennisetum glaucum x Pennisetum purpureum) for ethanol cellulosic
Fonte: Rev. colomb. biotecnol;14(1):146-156, ene.-jun. 2012. ilus, graf, tab.
Idioma: es.
Resumo: Evaluar la producción de etanol a partir de cultivos lignocelulósicos, específicamente pastos de rápido crecimiento en la región, constituye una alternativa a la demanda de biocombustibles. En la presente investigación se seleccionó el pasto Maralfalfa (Pennisetum glaucum x Pennisetum purpureum) utilizando el pretratamiento con ácido sulfúrico diluido a diferentes temperaturas (110, 130, 150, 170 y 190 °C) y concentraciones de ácido (0.8, 1.2 y 2.0% (p/p)), seguido de un proceso de hidrólisis enzimática utilizando celulasas y celobiosas comerciales y un proceso de hidrólisis y fermentación simultanea. La máxima producción de etanol obtenido fue 117 mg etanol/ g biomasa pretratada a 190 °C y 1,2 %(p/p) de ácido sulfúrico. El líquido hidrolizado fue caracterizado calculando el porcentaje de glucosa, xilosa y lignina solubilizadas y degradadas durante el pretratamiento.

The goliath grass (Pennisetum glaucum x Pennisetum purpureum) was pretreated with different sulfuric acid concentrations (0.8, 1.2 y 2.0% (w/w)) from low to high temperatures (110, 130, 150, 170 y 190 °C) followed by enzymatic hydrolysis and SSF of remaining solids. The maximum yield was 117 mg of ethanol/g biomass to 190 °C and 1.2 % (w/w) of sulfuric acid.
Descritores: Hidrólise
Pennisetum/crescimento & desenvolvimento
-Celobiose
Celulase
Celulose
Etanol/síntese química
Glucose
Lignina
Xilose
Responsável: CO326 - Departamento de Biología



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