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Texto completo SciELO Brasil
Richtzenhain, Leonardo José
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Id: biblio-889223
Autor: Favaro, Patricia Filippsen; Fernandes, Wilson Roberto; Reischak, Dilmara; Brandão, Paulo Eduardo; Silva, Sheila Oliveira de Souza; Richtzenhain, Leonardo José.
Título: Evolution of equine influenza viruses (H3N8) during a Brazilian outbreak, 2015
Fonte: Braz. j. microbiol;49(2):336-346, Apr.-June 2018. tab, graf.
Idioma: en.
Projeto: FAPESP-Brazil; . CNPq-Brazil; . CAPES/PROEX-Brazil.
Resumo: Abstract Equine influenza is one of the major respiratory infectious diseases in horses. An equine influenza virus outbreak was identified in vaccinated and unvaccinated horses in a veterinary school hospital in São Paulo, SP, Brazil, in September 2015. The twelve equine influenza viruses isolated belonged to Florida Clade 1. The hemagglutinin and neuraminidase amino acid sequences were compared with the recent isolates from North and South America and the World Organisation for Animal Health recommended Florida Clade 1 vaccine strain. The hemagglutinin amino acid sequences had nine substitutions, compared with the vaccine strain. Two of them were in antigenic site A (A138S and G142R), one in antigenic site E (R62K) and another not in antigenic site (K304E). The four substitutions changed the hydrophobicity of hemagglutinin. Three distinct genetic variants were identified during the outbreak. Eleven variants were found in four quasispecies, which suggests the equine influenza virus evolved during the outbreak. The use of an out of date vaccine strain or updated vaccines without the production of protective antibody titers might be the major contributing factors on virus dissemination during this outbreak.
Descritores: Variação Genética
Surtos de Doenças
Infecções por Orthomyxoviridae/veterinária
Evolução Molecular
Vírus da Influenza A Subtipo H3N8/isolamento & purificação
Doenças dos Cavalos/epidemiologia
Doenças dos Cavalos/virologia
-Orthomyxoviridae
Proteínas Virais/genética
Brasil/epidemiologia
Análise de Sequência de DNA
Infecções por Orthomyxoviridae/epidemiologia
Infecções por Orthomyxoviridae/virologia
Glicoproteínas de Hemaglutininação de Vírus da Influenza/genética
Substituição de Aminoácidos
Vírus da Influenza A Subtipo H3N8/classificação
Vírus da Influenza A Subtipo H3N8/genética
Genótipo
Cavalos
Hospitais Veterinários
Neuraminidase/genética
Limites: Animais
Responsável: BR1.1 - BIREME


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Id: biblio-871442
Autor: Greatti, Mariana Morena de Vieira Santos.
Título: Flora intermediária em mulheres em idade reprodutiva: aspectos inflamatórios, atividade de sialidases e carga bacteriana / Intermediate flora in women of reproductive age: inflammatory aspects, sialidase activity and bacterial load.
Fonte: Botucatu; s.n; 2014. 51 p.
Idioma: pt.
Tese: Apresentada a Universidade Estadual Paulista Júlio de Mesquita Filho. Faculdade de Medicina de Botucatu para obtenção do grau de Doutor.
Descritores: Citocinas
Neuraminidase/análise
Vagina/microbiologia
Vaginose Bacteriana/diagnóstico
Responsável: BR33.1 - Divisão Técnica de Biblioteca e Documentação
BR33.1


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Texto completo
Id: biblio-829259
Autor: Pontoriero, Andrea; Avaro, Martín; Benedetti, Estefania; Russo, Mara; Czech, Andrea; Periolo, Natalia; Campos, Ana; Zamora, Ana; Baumeister, Elsa.
Título: Surveillance of antiviral resistance markers in Argentina: detection of E119V neuraminidase mutation in a post-treatment immunocompromised patient
Fonte: Mem. Inst. Oswaldo Cruz;111(12):745-749, Dec. 2016. graf.
Idioma: en.
Projeto: National Agency of Scientific Research, Technology and Innovation of Argentina.
Resumo: Although vaccines are the best means of protection against influenza, neuraminidase inhibitors are currently the main antiviral treatment available to control severe influenza cases. One of the most frequent substitutions in the neuraminidase (NA) protein of influenza A(H3N2) viruses during or soon after oseltamivir administration is E119V mutation. We describe the emergence of a mixed viral population with the E119E/V mutation in the NA protein sequence in a post-treatment influenza sample collected from an immunocompromised patient in Argentina. This substitution was identified by a real-time reverse transcriptase polymerase chain reaction (RT-PCR) protocol and was confirmed by direct Sanger sequencing of the original sample. In 2014, out of 1140 influenza samples received at the National Influenza Centre, 888 samples (78%) were A(H3N2) strains, 244 (21.3%) were type B strains, and 8 (0.7%) were A(H1N1)pdm09 strains. Out of 888 A(H3N2) samples, 842 were tested for the E119V substitution by quantitative RT-PCR: 841 A(H3N2) samples had the wild-type E119 genotype and in one sample, a mixture of viral E119/ V119 subpopulations was detected. Influenza virus surveillance and antiviral resistance studies can lead to better decisions in health policies and help in medical treatment planning, especially for severe cases and immunocompromised patients.
Descritores: Antivirais/uso terapêutico
Vírus da Influenza A Subtipo H3N2/efeitos dos fármacos
Influenza Humana/epidemiologia
Influenza Humana/virologia
Neuraminidase/genética
Oseltamivir/uso terapêutico
Proteínas Virais/genética
-Argentina/epidemiologia
Hospedeiro Imunocomprometido
Vírus da Influenza A Subtipo H3N2
Influenza Humana/tratamento farmacológico
Mutação
Reação em Cadeia da Polimerase em Tempo Real
Limites: Seres Humanos
Masculino
Feminino
Lactente
Pré-Escolar
Criança
Adolescente
Adulto
Meia-Idade
Idoso
Idoso de 80 Anos ou mais
Adulto Jovem
Responsável: BR1.1 - BIREME


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Texto completo SciELO Saúde Pública
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Id: lil-788101
Autor: Shield, Kevin D.; Monteiro, Maristela; Roerecke, Michael; Smith, Blake; Rehm, Jürgen.
Título: Alcohol consumption and burden of disease in the Americas in 2012: implications for alcohol policy / El consumo de alcohol y la carga de morbilidad en la región de las Américas en el 2012: implicaciones para las políticas relacionadas con el consumo de alcohol
Fonte: Rev. panam. salud pública = Pan am. j. public health;38(6):442-449, nov.-dic. 2015. tab.
Idioma: en.
Resumo: OBJECTIVE:To describe the volume and patterns of alcohol consumption up to and including 2012, and to estimate the burden of disease attributable to alcohol consumption as measured in deaths and disability-adjusted life years (DALYs) lost in the Americas in 2012. METHODS: Measures of alcohol consumption were obtained from the World Health Organization (WHO) Global Information System on Alcohol and Health (GISAH). The burden of alcohol consumption was estimated in both deaths and DALYs lost based on mortality data obtained from WHO, using alcohol-attributable fractions. Regional groupings for the Americas were based on the WHO classifications for 2004 (according to child and adult mortality). RESULTS: Regional variations were observed in the overall volume of alcohol consumed, the proportion of the alcohol market attributable to unrecorded alcohol consumption, drinking patterns, prevalence of drinking, and prevalence of heavy episodic drinking, with inhabitants of the Americas consuming more alcohol (8.4 L of pure alcohol per adult in 2012) compared to the world average. The Americas also experienced a high burden of disease attributable to alcohol consumption (4.7% of all deaths and 6.7% of all DALYs lost), especially in terms of injuries attributable to alcohol consumption. CONCLUSIONS: Alcohol is consumed in a harmful manner in the Americas, leading to a high burden of disease, especially in terms of injuries. New cost-effective alcohol policies, such as increasing alcohol taxation, increasing the minimum legal age to purchase alcohol, and decreasing the maximum legal blood alcohol content while driving, should be implemented to decrease the harmful consumption of alcohol and the resulting burden of disease.

OBJETIVO:Describir el volumen y los modelos de consumo de alcohol hasta el año 2012 incluido, y calcular la carga de morbilidad atribuible al consumo de alcohol medida según el número de defunciones y los años de vida ajustados en función de la discapacidad (AVAD) perdidos en la Región de las Américas en el 2012. MÉTODOS: Los datos sobre el consumo de alcohol se obtuvieron a partir del Sistema Mundial de Información sobre el Alcohol y la Salud (GISAH, por sus siglas en inglés) de la Organización Mundial de la Salud (OMS). La carga del consumo de alcohol se calculó según la mortalidad y según los AVAD perdidos con base en los datos de mortalidad obtenidos de la OMS, tomando en consideración las fracciones atribuibles al alcohol. La división en subregiones se basó en las clasificaciones de la OMS del año 2004 (según la mortalidad en niños y adultos). RESULTADOS: Se observaron variaciones regionales en el volumen total de alcohol consumido, la proporción del mercado del alcohol atribuible al consumo de alcohol no registrado, los hábitos de consumo, la prevalencia del consumo y la prevalencia de los episodios de consumo excesivo de alcohol. Los habitantes de la Región de las Américas consumieron más alcohol (8,4 litros de alcohol puro por adulto en el 2012) en comparación con el promedio mundial. La Región también experimentó una alta carga de morbilidad atribuible al consumo de alcohol (4,7% de las defunciones y 6,7% de los AVAD perdidos), especialmente en forma de lesiones atribuibles al consumo de alcohol. CONCLUSIONES: El alcohol se consume de una manera perjudicial en la Región de las Américas y ello comporta una alta carga de morbilidad, especialmente en forma de lesiones. Con objeto de disminuir el consumo perjudicial de bebidas alcohólicas y la carga de morbilidad resultante, es preciso introducir nuevas políticas en materia de consumo de alcohol que sean eficaces en función de los costos, tales como el incremento de los impuestos sobre el alcohol, el aumento de la edad mínima legal para adquirir alcohol, y la disminución de la concentración máxima legal de alcohol en sangre mientras se conduce.
Descritores: Proteínas de Bactérias/química
Neuraminidase/química
Streptococcus pneumoniae/enzimologia
Fatores de Virulência/química
-Sítios de Ligação
Proteínas de Bactérias/metabolismo
Lactose/análogos & derivados
Lactose/metabolismo
Modelos Moleculares
Neuraminidase/metabolismo
Ligação Proteica
Dobramento de Proteína
Estrutura Terciária de Proteína
Ácidos Siálicos/metabolismo
Streptococcus pneumoniae/química
Fatores de Virulência/metabolismo
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


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Rudge, Marilza Vieira Cunha
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Id: lil-770148
Autor: Ferreira, Carolina Sanitá Tafner; Marconi, Camila; Parada, Cristina Maria de Lima Garcia; Duarte, Marli Teresinha Cassamassimo; Gonçalves, Ana Paula Oliveira; Rudge, Marilza Vieira Cunha; Silva, Márcia Guimarães da.
Título: Bacterial vaginosis in pregnant adolescents: proinflammatory cytokine and bacterial sialidase profile. Cross-sectional study / Vaginose bacteriana em gestantes adolescentes: perfil de citocinas proinflamatórias e sialidases bacterianas. Estudo transversal
Fonte: Säo Paulo med. j;133(6):465-470, Nov.-Dec. 2015. tab, graf.
Idioma: en.
Projeto: Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq).
Resumo: ABSTRACT CONTEXT AND OBJECTIVE: Bacterial vaginosis occurs frequently in pregnancy and increases susceptibility to sexually transmitted infections (STI). Considering that adolescents are disproportionally affected by STI, the aim of this study was to evaluate the cervicovaginal levels of interleukin (IL)-1 beta, IL-6, IL-8 and bacterial sialidase in pregnant adolescents with bacterial vaginosis. DESIGN AND SETTING: Cross-sectional study at mother and child referral units in Belém, Pará, Brazil. METHODS: Vaginal samples from 168 pregnant adolescents enrolled were tested for trichomoniasis and candidiasis. Their vaginal microbiota was classified according to the Nugent criteria (1991) as normal, intermediate or bacterial vaginosis. Cervical infection due to Chlamydia trachomatisand Neisseria gonorrhoeae was also assessed. Cytokine and sialidase levels were measured, respectively, using enzyme-linked immunosorbent assays and MUAN conversion in cervicovaginal lavages. Forty-eight adolescents (28.6%) were excluded because they tested positive for some of the infections investigated. The remaining 120 adolescents were grouped according to vaginal flora type: normal (n = 68) or bacterial vaginosis (n = 52). Their cytokine and sialidase levels were compared between the groups using the Mann-Whitney test (P < 0.05). RESULTS: The pregnant adolescents with bacterial vaginosis had higher levels of IL-1 beta, IL-6 and IL-8 (P < 0.05). Sialidase was solely detected in 35 adolescents (67.2%) with bacterial vaginosis. CONCLUSIONS: Not only IL-1 beta and sialidase levels, but also IL-6 and IL-8 levels are higher in pregnant adolescents with bacterial vaginosis, thus indicating that this condition elicits a more pronounced inflammatory response in this population, which potentially increases vulnerability to STI acquisition.

RESUMO CONTEXTO E OBJETIVO: A vaginose bacteriana é uma condição, comum em gestantes, que aumenta a susceptibilidade a infecções sexualmente transmissíveis (IST). Considerando que adolescentes são desproporcionalmente afetadas por IST, o objetivo deste estudo foi avaliar os níveis cervicovaginais de interleucina (IL)-1 beta, IL-6, IL-8 e sialidases bacterianas em gestantes adolescentes com vaginose bacteriana. DESENHO DO ESTUDO E LOCAL: Estudo transversal em Unidade de Referência Materno Infantil (UREMIA), Belém, Pará, Brasil. MÉTODOS: Amostras vaginais das 168 gestantes adolescentes incluídas foram testadas para tricomoníase e candidíase e a microbiota vaginal foi classificada em normal, intermediária e vaginose bacteriana, segundo os critérios de Nugent (1991). Infecções cervicais por Chlamydia trachomatis eNeisseria gonorrhoeae também foram avaliadas. Os níveis de citocinas e sialidades foram quantificados, respectivamente, por método imunoenzimático e pela conversão do MUAN nos lavados cervicovaginais. Foram excluídas 48 (28,6%) adolescentes positivas para alguma das infecções investigadas. As 120 gestantes remanescentes foram agrupadas de acordo com o padrão de flora vaginal em: normal (n = 68) e vaginose bacteriana (n = 52). Níveis de citocinas e sialidases foram comparados pelo teste de Mann-Whitney, P < 0,05. RESULTADOS: As gestantes adolescentes com vaginose bacteriana entre os grupos apresentaram níveis aumentados de IL-1 beta, IL-6 and IL-8 (P < 0,05). Sialidases foram exclusivamente detectadas em 35 (67,2%) adolescentes com vaginose bacteriana. CONCLUSÕES: Não apenas a IL-1 beta e as sialidases estão aumentadas em gestantes adolescentes com vaginose bacteriana, mas também IL-6 e IL-8, indicando resposta inflamatória mais pronunciada dessa alteração de microbiota nesta população, potencializando a vulnerabilidade à aquisição de IST.
Descritores: Interleucinas/análise
Neuraminidase/análise
Vaginose Bacteriana/patologia
-Estudos Transversais
Colo do Útero/microbiologia
Ensaio de Imunoadsorção Enzimática
Interleucina-1/análise
/análise
INTERLEUKIN-ABDOMEN, ACUTE/análise
/análise
INTERLEUKIN-ABDOMINAL NEOPLASMS/análise
Medição de Risco
Fatores de Risco
Fatores Socioeconômicos
Estatísticas não Paramétricas
Doenças Bacterianas Sexualmente Transmissíveis/microbiologia
Vagina/microbiologia
Limites: Adolescente
Feminino
Seres Humanos
Gravidez
Adulto Jovem
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


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Texto completo SciELO Brasil
Mello, Wyller Alencar de
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Id: lil-728806
Autor: Oliveira, Maria José Couto; Motta, Fernando do Couto; Siqueira, Marilda M; Resende, Paola Cristina; Born, Priscilla da Silva; Souza, Thiago Moreno L; Mesquita, Milene; Oliveira, Maria de Lourdes Aguiar; Carney, Sharon; Mello, Wyller Alencar de; Magalhães, Vera.
Título: Molecular findings from influenza A(H1N1)pdm09 detected in patients from a Brazilian equatorial region during the pandemic period
Fonte: Mem. Inst. Oswaldo Cruz;109(7):912-917, 11/2014. tab, graf.
Idioma: en.
Resumo: After the World Health Organization officially declared the end of the first pandemic of the XXI century in August 2010, the influenza A(H1N1)pdm09 virus has been disseminated in the human population. In spite of its sustained circulation, very little on phylogenetic data or oseltamivir (OST) resistance is available for the virus in equatorial regions of South America. In order to shed more light on this topic, we analysed the haemagglutinin (HA) and neuraminidase (NA) genes of influenza A(H1N1)pdm09 positive samples collected during the pandemic period in the Pernambuco (PE), a northeastern Brazilian state. Complete HA sequences were compared and amino acid changes were related to clinical outcome. In addition, the H275Y substitution in NA, associated with OST resistance, was investigated by pyrosequencing. Samples from PE were grouped in phylogenetic clades 6 and 7, being clustered together with sequences from South and Southeast Brazil. The D222N/G HA gene mutation, associated with severity, was found in one deceased patient that was pregnant. Additionally, the HA mutation K308E, which appeared in Brazil in 2010 and was only detected worldwide the following year, was identified in samples from hospitalised cases. The resistance marker H275Y was not identified in samples tested. However, broader studies are needed to establish the real frequency of resistance in this Brazilian region.
Descritores: Hemaglutininas/genética
Vírus da Influenza A Subtipo H1N1/genética
Influenza Humana/epidemiologia
Neuraminidase/genética
Pandemias
-Antivirais/uso terapêutico
Biomarcadores/análise
Brasil/epidemiologia
Farmacorresistência Viral/fisiologia
Frequência do Gene/genética
Vírus da Influenza A Subtipo H1N1/classificação
Vírus da Influenza A Subtipo H1N1/patogenicidade
Influenza Humana/virologia
Mutação/genética
Oseltamivir/uso terapêutico
Filogenia
RNA Viral/análise
Análise de Sequência de DNA/métodos
Virulência
Fatores de Virulência/genética
Limites: Feminino
Seres Humanos
Gravidez
Responsável: BR1.1 - BIREME


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Texto completo SciELO Brasil
Texto completo
Id: lil-714878
Autor: Jefferson, Tom; Jones, Mark A.; Doshi, Peter; Del Mar, Chris B.; Hama, Rokuro; Thompson, Matthew; Spencer, Elizabeth A.; Onakpoya, Igho; Mahtani, Kamal R.; Nunan, David Nunan; Howick, Jeremy; Heneghan, Carl J..
Título: Neuraminidase inhibitors for preventing and treating influenza in healthy adults and children
Fonte: Säo Paulo med. j;132(4):256-257, 07/2014.
Idioma: en.
Resumo: BACKGROUND: Neuraminidase inhibitors (NIs) are stockpiled and recommended by public health agencies for treating and preventing seasonal and pandemic influenza. They are used clinically worldwide. OBJECTIVE: To describe the potential benefits and harms of NIs for influenza in all age groups by reviewing all clinical study reports of published and unpublished randomised, placebo-controlled trials and regulatory comments. METHODS Search methods: We searched trial registries, electronic databases (to 22 July 2013) and regulatory archives, and corresponded with manufacturers to identify all trials. We also requested clinical study reports. We focused on the primary data sources of manufacturers but we checked that there were no published randomised controlled trials (RCTs) from non-manufacturer sources by running electronic searches in the following databases: the Cochrane Central Register of Controlled Trials (CENTRAL), MEDLINE, MEDLINE (Ovid), EMBASE, Embase.com, PubMed (not MEDLINE), the Database of Reviews of Effects, the NHS Economic Evaluation Database and the Health Economic Evaluations Database. Selection criteria: Randomised, placebo-controlled trials on adults and children with confirmed or suspected exposure to naturally occurring influenza. Data collection and analysis: We extracted clinical study reports and assessed risk of bias using purpose-built instruments. We analysed the effects of zanamivir and oseltamivir on time to first alleviation of symptoms, influenza outcomes, complications, hospitalisations and adverse events in the intention-to-treat (ITT) population. All trials were sponsored by the manufacturers. MAIN RESULTS: We obtained 107 clinical study reports from the European Medicines Agency (EMA), GlaxoSmithKline and Roche. We accessed comments by the US Food and Drug Administration (FDA), EMA and Japanese regulator. We included 53 trials in Stage 1 (a judgement of appropriate study design) and 46 in Stage ...
Descritores: Antivirais/uso terapêutico
Inibidores Enzimáticos/uso terapêutico
Influenza Humana/tratamento farmacológico
Influenza Humana/prevenção & controle
Neuraminidase/antagonistas & inibidores
Oseltamivir/uso terapêutico
Zanamivir/uso terapêutico
Limites: Seres Humanos
Tipo de Publ: Comentário
Responsável: BR1.1 - BIREME


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Lunge, Vagner Ricardo
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Id: lil-676978
Autor: Memórias do Instituto Oswaldo Cruz; Marx, Camila; Gregianini, Tatiana Schäffer; Lehmann, Fernanda Kieling Moreira; Lunge, Vagner Ricardo; Carli, Silvia de; Dambrós, Bibiana Paula; Tumioto, Gabriela Luchiari; Seadi, Claudete; Fonseca, André Salvador Kazantzi; Ikuta, Nilo.
Título: Oseltamivir-resistant influenza A(H1N1)pdm09 virus in southern Brazil
Fonte: Mem. Inst. Oswaldo Cruz;108(3):392-394, maio 2013.
Idioma: en.
Projeto: FAPERGS.
Resumo: The neuraminidase (NA) genes of A(H1N1)pdm09 influenza virus isolates from 306 infected patients were analysed. The circulation of oseltamivir-resistant viruses in Brazil has not been reported previously. Clinical samples were collected in the state of Rio Grande do Sul (RS) from 2009-2011 and two NA inhibitor-resistant mutants were identified, one in 2009 (H275Y) and the other in 2011 (S247N). This study revealed a low prevalence of resistant viruses (0.8%) with no spread of the resistant mutants throughout RS.
Descritores: Antivirais/farmacologia
Farmacorresistência Viral/genética
Vírus da Influenza A Subtipo H1N1/efeitos dos fármacos
Mutação
Neuraminidase/genética
Oseltamivir/farmacologia
-Brasil
Vírus da Influenza A Subtipo H1N1/enzimologia
Vírus da Influenza A Subtipo H1N1/genética
Testes de Sensibilidade Microbiana
Reação em Cadeia da Polimerase Via Transcriptase Reversa
RNA Viral/genética
Limites: Seres Humanos
Responsável: BR1.1 - BIREME


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Texto completo SciELO Costa Rica
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Id: lil-637654
Autor: Borraz-Argüello, María del Tránsito; Santos-López, Gerardo; Vallejo-Ruiz, Verónica; Herrera-Camacho, Irma; Reyes-Leyva, Julio.
Título: Caracterización biológica de tres aislamientos naturales del Rubulavirus porcino (México)
Fonte: Rev. biol. trop;56(2):487-499, jun. 2008. ilus, graf, tab.
Idioma: es.
Resumo: Biological characterization of three natural isolates of the porcine rubulavirus (Mexico). Porcine rubulavirus (PoRV) produces a neurological and reproductive syndrome in pigs called the blue-eye disease, known only from Mexico. Several isolates were grouped by the main symptoms presented during outbreaks: a) neurotropic in piglets, b) broadly neurotropic in piglets and gonadotropic in adults, and c) gonadotropic in adults. We studied some biological properties of three strains, which fall in one of each virus group: La Piedad Michoacán (LPM) and Producción Animal Cerdos 1 (PAC1) and 3 (PAC3), respectively. The analyzed viral properties are mainly related with the trans-membrane hemagglutinin-neuraminidase (HN) and fusion (F) proteins, such as cytopathic effect, hemolysis, hemagglutinating (HA) and neuraminidase (NA) activities. In the infection assays PAC1 strain presented the highest fusogenicity level; however, the most cytolytic strain was PAC3. In addition, HA and NA activities and viral genome of PAC3 strain was detected in supernatants during cell infection earlier than in the other two strains, which shows that PAC3 virions release from the host cell earlier than LPM and PAC1. Experimental determination in purified viruses shows that PAC3 presented a higher HA and NA activities; however, PAC1 shows other interesting properties, such as a high thermostability of HN and differences about substrate profile respect to LPM and PAC3. Our data suggest that NA activity is associated with the virulence of RVP. Rev. Biol. Trop. 56 (2): 487-499. Epub 2008 June 30.

El Rubulavirus porcino causa un síndrome neurológico y reproductivo en cerdos, hasta ahora reportado sólo en México. Los virus aislados se agrupan de acuerdo con los síntomas principales observados durante los brotes en: a) neutrópicos en lechones, b) neurotrópicos en lechones/gonadotrópicos en adultos y c) gonadotrópicos en adultos. En este trabajo se estudiaron tres cepas: La Piedad Michoacán (LPM) y Producción Animal "Cerdos" 1 (PAC1) y 3 (PAC3), ubicadas respectivamente en cada grupo. Las propiedades estudiadas se relacionan principalmente con dos proteínas de la envoltura viral, la hemaglutinina-neuraminidasa (HN) y la proteína de fusión (F). Se cuantificaron el efecto citopático y las actividades de hemólisis, hemaglutinación (HA) y neuraminidasa (NA). En cultivo celular la cepa PAC1 presentó una mayor actividad fusogénica, sin embargo PAC3 presentó la mayor actividad citolítica. La cepa PAC3 fue la primera en ser detectada en sobrenadante de células infectadas (HA, NA y genoma), lo que muestra que sus viriones son liberados al medio antes que las otras dos cepas. PAC3 tuvo las actividades más altas de HA y NA, sin embargo, PAC1 presentó una mayor termoestabilidad en estas actividades de HN y un perfil de substrato algo distinto de los observados para LPM y PAC3. Estos datos sugieren que la actividad de NA está relacionada con la virulencia del RVP.
Descritores: Infecções por Rubulavirus/virologia
Rubulavirus/isolamento & purificação
Doenças dos Suínos/virologia
-Hemaglutinação por Vírus
Proteína HN/metabolismo
México
Neuraminidase/metabolismo
Rubulavirus/enzimologia
Rubulavirus/genética
Rubulavirus/patogenicidade
Suínos
Limites: Animais
Responsável: CR1.1 - BINASSS - Biblioteca Nacional de Salud y Seguridad Social


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Id: lil-633829
Autor: Gil, José; Cimino, Rubén; López Quiroga, Inés; Cajal, Silvana; Acosta, Norma; Juaréz, Marisa; Zacca, Rosa; Orellana, Viviana; Krolewiecki, Alejandro; Diosque, Patricio; Nasser, Julio.
Título: Reactividad del antígeno GST-SAPA de Trypanosoma cruzi frente a sueros de pacientes con enfermedad de Chagas y leishmaniasis / Reactivity of GST-SAPA antigen of Trypanosoma cruzi against sera from patients with Chagas disease and leishmaniasis
Fonte: Medicina (B.Aires);71(2):113-119, mar.-abr. 2011. graf, mapas, tab.
Idioma: es.
Projeto: Consejo Nacional de Investigaciones Científicas y Técnicas; . IRD. DSF; . Universidad Nacional de Salta. Consejo de Investigación; . Secretaria de Ciencia y Técnica de la Nación; . European Union Seventh Framework Programme. PICTo-2006 (Préstamo BID 1728 OC AR PICTo 36714).
Resumo: El diagnóstico serológico de la infección producida por Trypanosoma cruzi es de especial relevancia dado que los métodos parasitológicos tienen, en las fases indeterminada y crónica, una sensibilidad limitada. El antígeno SAPA fue usado en diversos estudios y demostró ser un buen candidato para el diagnóstico de la infección por T. cruzi. La enfermedad de Chagas y la leishmaniasis son endémicas en el norte de Salta, con posibles zonas de solapamiento. Este hecho suele dar lugar a infecciones mixtas T. cruzi-Leishmania spp., con la consecuente probabilidad de diagnóstico cruzado cuando se usan antígenos no específicos. Se evaluó la reactividad del antígeno GST-SAPA en la prueba de ELISA (ELISA-SAPA) frente a sueros de personas infectadas por T. cruzi (n = 154), con leishmaniasis (n = 66), infecciones mixtas (n = 29) y controles negativos (n = 28), usando como pruebas de referencia para el diagnóstico de la infección por T. cruzi kits comerciales de ELISA y HAI. Se calculó la sensibilidad, especificidad e índice de concordancia kappa de la prueba de ELISA-SAPA, para la detección de infección por T. cruzi. Entre los sueros de pacientes con leishmaniasis estudiados se detectó un 30.5% de infecciones mixtas. Para la detección de infección por T. cruzi, ELISA-SAPA mostró una sensibilidad del 97.1% (intervalo de confianza del 95%: 94.5-99.9), una especificidad del 100% (intervalo de confianza del 95%: 99.5-100) y un índice de concordancia kappa de 96 (intervalo de confianza del 95%:93-99%), comparado con las pruebas serológicas comerciales. Los valores de sensibilidad, especificidad y concordancia calculados muestran una alta eficiencia de ELISA-SAPA.

Serologic diagnosis of Trypanosoma cruzi infection is important due to the limited sensitivity of direct parasitologic methods for diagnosis in the indeterminate and chronic phases of disease. SAPA antigen has been used in several studies and has been shown to be a good marker for use in the diagnosis of T. cruzi infection. Chagas disease and leishmaniasis are endemic in northern Salta with overlapping zones of transmission, which frequently leads to T. cruzi-Leishmania spp. mixed infections. Diagnosis is complicated by the fact that there is significant cross-reactivity when non-specific antigens are used. We evaluated the reactivity of GST-SAPA antigen in the ELISA test (ELISA-SAPA) against sera from persons infected with T. cruzi (n = 154), leishmaniasis (n = 66), mixed infections (29), and healthy controls (n = 28) using commercial ELISA and IHA kits as reference tests. For ELISA-SAPA the sensitivity, specificity and kappa index were calculated for detection of T. cruzi infection. Among sera from patients infected with leishmaniasis, 30.5% of co-infections were detected. ELISA-SAPA sensitivity was 97.1% (confidence interval 95%: 94.5-99.9), specificity was 100% (confidence interval 95%: 99.4-100), and kappa index was 96% (confidence interval 95%: 93-99%), for detection of T. cruzi infection. Sensitivity, specificity and kappa indices have shown a high efficiency of ELISA-SAPA.
Descritores: Anticorpos Antiprotozoários/sangue
Antígenos de Protozoários
Doença de Chagas/diagnóstico
Glicoproteínas
Leishmaniose Visceral/imunologia
Neuraminidase
Trypanosoma cruzi/imunologia
-Antígenos de Protozoários/imunologia
Doença de Chagas/imunologia
Reações Cruzadas/imunologia
Ensaio de Imunoadsorção Enzimática
Proteínas Recombinantes
Proteínas Recombinantes/imunologia
Sensibilidade e Especificidade
Especificidade da Espécie
Testes Sorológicos/métodos
Limites: Seres Humanos
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: AR1.2 - Instituto de Investigaciónes Epidemiológicas



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