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Id: biblio-1056430
Autor: Wang, Jing-yuan; Wang, Xue-min; Xu, Xiao-yan; Li, Shi-rong; Liu, Xiu-lan.
Título: Expression and significance of CK5/6, P63, P40, CK7, TTF-1, NapsinA, CD56, Syn and CgA in biopsy specimen of squamous cell carcinoma, adenocarcinoma and small cell lung carcinoma / Expresión y significado de CK5/6, P63, P40, CK7, TTF-1, NapsinA, CD56 Syn y CgA en muestras de biopsia de carcinoma de células escamosas, adenocarcinoma y carcinoma de células pequeñas de pulmón
Fonte: Int. j. morphol;38(2):247-251, abr. 2020. tab, graf.
Idioma: en.
Resumo: Nine tumor and various potential biomarkers were measured and combined the information to diagnose disease, all patients accepted fiber bronchoscopy brush liquid based cytologyand histopathology examination in order to reliably detect lung cancer. The samples from 314 Chinese lung cancer patients were obtained and CK5/6, P63, P40, CK7, TTF-1, NapsinA CD56, Syn and CgA were measured with the immunohistochemical SP method and analyzed correlation of the expression of these markers with pathological and clinical features of squamous cell carcinoma, adenocarcinoma, and small cell lung carcinoma. Squamous cell carcinoma, adenocarcinoma and small cell carcinoma were 61 cases, 114 cases and 139 cases,CK5/6 and P63 expression were more frequent in squamous cell carcinoma, with sensitivity and specificity of 77.05 % and 96.44 %, 83.61 % and 88.93 %,and compared with adenocarcinoma and small cell carcinoma difference was statistically significant (P<0.05), The incidences of a positive P40 expression were 100 % in squamous cell carcinoma, with specificity of 98.81 %.CK7, TTF-1 and NapsinA expression were more frequent in adenocarcinoma, with sensitivity and specificity of 85.09 % and 78.69 %, 79.82 % and 93.44 %, 56.14 % and 95.08 %, and compared with squamous cell carcinoma and small cell carcinoma difference was statistically significant (P<0.05). TTF-1, Syn, CgA and CD56 expression were more frequent in adenocarcinoma, with sensitivity and specificity of 86.33 % and 93.44 %, 89.21 % and 98.36 %, 74.10 % and 100 %, 96.40 % and 96.72 %. The combined detection of CK5/6, P63 and P40 were more useful and specific in differentiating squamous cell carcinoma. CK7, TTF-1 and NapsinA were more useful and specific in differentiating lung adenocarcinoma. The impaired CD56, TTF-1, Syn and CgA reflects the progression of small cell lung cancer.

Se midieron tumores y utilizaron nueve biomarcadores potenciales y se analizó la información para diagnosticar la enfermedad. A todos los pacientes se les realizó citología en líquido con broncoscopía de fibra y examen histopatológico para detectar de manera confiable el cáncer pulmonar. Se obtuvieron muestras de 314 pacientes chinos con cáncer de pulmón y CK5 / 6, P63, P40, CK7, TTF-1, Napsina A, CD56, Syn y CgA se midieron a través de histoquímica SP y analizaron la correlación de la expresión de estos marcadores con características patológicas y clínicas de carcinoma de células escamosas, adenocarcinoma y carcinoma de células pequeñas en el cáncer de pulmón. El carcinoma de células escamosas, el adenocarcinoma y el carcinoma de células pequeñas fueron 61 casos, 114 casos y 139 casos, respectivamente, la expresión de CK5 / 6 y P63 fueron más frecuentes en el carcinoma de células escamosas, con una sensibilidad y especificidad del 77,05 % y 96,44 %, 83,61 % y 88,93 %, y en comparación con el adenocarcinoma y el carcinoma de células pequeñas, la diferencia fue estadísticamente significativa (P <0,05). La incidencia de ap la expresión positiva P40 fue del 100 % en el carcinoma de células escamosas, con una especificidad del 98,81 %. La expresión de CK7, TTF-1 y NapsinA fueron más frecuentes en el adenocarcinoma, con una sensibilidad y especificidad del 85,09 % y 78,69 %, 79,82 % y 93,44 %, 56,14 % y 95,08 %, y en comparación con el carcinoma de células escamosas y la diferencia de carcinoma de células pequeñas fue estadísticamente significativa (P <0,05) .TTF-1, Syn, CgA y la expresión de CD56 fueron más frecuentes en adenocarcinoma, con sensibilidad y especificidad de 86.33 % y 93.44 %, 89.21 % y 98.36 %, 74.10 % y 100 %, 96.40 % y 96.72 %. La detección combinada de CK5 / 6, P63 y P40 fue más útil y específica en la diferenciación del carcinoma de células escamosas. CK7, TTF-1 y NapsinA fueron más útiles y específicos para diferenciar el adenocarcinoma de pulmón. El deterioro de CD56, TTF-1, Syn y CgA refleja la progresión del cáncer de pulmón de células pequeñas.
Descritores: Carcinoma/metabolismo
Carcinoma/patologia
Neoplasias Pulmonares/metabolismo
Neoplasias Pulmonares/patologia
-Fragmentos de Peptídeos/metabolismo
Fatores de Transcrição/metabolismo
Imuno-Histoquímica
Carcinoma de Células Escamosas/metabolismo
Carcinoma de Células Escamosas/patologia
Adenocarcinoma/metabolismo
Adenocarcinoma/patologia
Biomarcadores Tumorais/metabolismo
Ácido Aspártico Endopeptidases/metabolismo
Sensibilidade e Especificidade
Carcinoma de Células Pequenas/metabolismo
Carcinoma de Células Pequenas/patologia
Antígeno CD56/metabolismo
Proteínas Supressoras de Tumor/metabolismo
Queratinas Tipo II/metabolismo
Queratina-7/metabolismo
Fator Nuclear 1 de Tireoide/metabolismo
Limites: Humanos
Responsável: CL1.1 - Biblioteca Central


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Id: biblio-829257
Autor: Nunes, Renata Rachide; Costa, Marina dos Santos; Santos, Bianca dos Reis; Fonseca, Amanda Luisa da; Ferreira, Lorena Sales; Chagas, Rafael Cesar Russo; Silva, Alisson Marques da; Varotti, Fernando de Pilla; Taranto, Alex Gutterres.
Título: Successful application of virtual screening and molecular dynamics simulations against antimalarial molecular targets
Fonte: Mem. Inst. Oswaldo Cruz;111(12):721-730, Dec. 2016. tab, graf.
Idioma: en.
Projeto: FAPEMIG; . FAPEMIG; . FAPEMIG; . CAPES.
Resumo: The main challenge in the control of malaria has been the emergence of drug-resistant parasites. The presence of drug-resistant Plasmodium sp. has raised the need for new antimalarial drugs. Molecular modelling techniques have been used as tools to develop new drugs. In this study, we employed virtual screening of a pyrazol derivative (Tx001) against four malaria targets: plasmepsin-IV, plasmepsin-II, falcipain-II, and PfATP6. The receiver operating characteristic curves and area under the curve (AUC) were established for each molecular target. The AUC values obtained for plasmepsin-IV, plasmepsin-II, and falcipain-II were 0.64, 0.92, and 0.94, respectively. All docking simulations were carried out using AutoDock Vina software. The ligand Tx001 exhibited a better interaction with PfATP6 than with the reference compound (-12.2 versus -6.8 Kcal/mol). The Tx001-PfATP6 complex was submitted to molecular dynamics simulations in vacuum implemented on an NAMD program. The ligand Tx001 docked at the same binding site as thapsigargin, which is a natural inhibitor of PfATP6. Compound TX001 was evaluated in vitro with a P. falciparum strain (W2) and a human cell line (WI-26VA4). Tx001 was discovered to be active against P. falciparum (IC50 = 8.2 µM) and inactive against WI-26VA4 (IC50 > 200 µM). Further ligand optimisation cycles generated new prospects for docking and biological assays.
Descritores: Antimaláricos/química
Ácido Aspártico Endopeptidases/química
Cisteína Endopeptidases/química
Simulação de Dinâmica Molecular
Proteínas de Protozoários/química
Tapsigargina/química
-Biologia Computacional/métodos
Terapia de Alvo Molecular/métodos
Limites: Humanos
Responsável: BR1.1 - BIREME


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Id: lil-729647
Autor: Assis Leitão, Luana Couto; da S. Simões, Mônica Oliveira; Oliveira Simões, Andrezza Eliab; Costa Alves, Bruna; Carvalho Barbosa, Igor; Barreto Pinto, Marlla Emanuella.
Título: Judicialização da saúde na garantia do acesso ao medicamento / The judicialisation of health as a means ensuring access to medicines / La Judicialización de la salud como garantía de acceso a medicamentos
Fonte: Rev. salud pública;16(3):361-370, 2012. ilus, tab.
Idioma: pt.
Resumo: Objetivo Visando conhecer o impacto das demandas judiciais sobre a organização dos serviços públicos de saúde, realizou-se uma revisáo sistemática com enfoque na "judicialização da saúde" para fornecimento de medicamentos. Métodos Foram analisados artigos originais publicados no período de 2007 a 2011, na literatura nacional e internacional, resultando no total de 49239 artigos disponíveis nas bases de dados Science Direct e BIREME. Resultados A pesquisa indicou predominância da bibliografia proveniente do Brasil, principalmente do sudeste, bem como de estudo realizado na Colômbia. Discursáo Dentre os pleitos, configuraram-se como principais agravos relatados as doenças crônicas, podendo-se citar: diabetes, hipertensáo, cânceres e artrite reumatóide. Por serem afecções parte de programas específicos do Sistema Único de Saúde, a dificuldade de acesso a esses fármacos e consequente judicialização da saúde demonstrou a fragilidade das políticas públicas existentes. Conclusão Por fim, conclui-se que a via judicial, apesar de ser uma estratégia para garantir o acesso ao medicamento, apresenta inabilidade para lidar com o julgamento das ações e gera, dessa forma, distorções no fluxo dos sistemas públicos.

Objective A systematic review, focusing on the judicialisation of health regarding gaining access to medicines, was aimed at understanding the impact of lawsuits on the organisation of public health services. Method Original articles published between 2007 and 2011 in the pertinent national and international literature were analysed, resulting in 49,239 articles being found in Science Direct and BIREME databases. Results The survey indicated a predominance of literature from Brazil, mainly the southeast, as well as a study from Colombia. Discussion The aforementioned chronic disease-related claims involved diabetes, high blood pressure, cancer and rheumatoid arthritis. Forming part of specific Unified Healthcare System programmes highlighted the difficulty in gaining access to the appropriate medicine and consequent health judicialisation demonstrated the fragility of existing public policy. Conclusion It was concluded that the courts (despite being a strategy for ensuring access to medicine) were unable to deal with the current spate of lawsuits, thereby leading to disruption regarding the flow of public systems.

Objetivo El estudio tiene como objetivo evaluar el impacto de las demandas judiciales sobre la organización de los servicios públicos de salud, mediante la realización de una revisión sistemática centrada en el uso de los tribunales para el suministro de medicamentos. Método Fueron identificados 49239 artículos en las bases de datos Science Direct e BIREME. Resultado El estudio indicó que la mayor parte de la bibliografía es de Brasil, con uno estudio en Colombia. Discusión Aparecen como los principales trastornos de salud relatados a las enfermedades crónicas, se pueden citar: la diabetes, la hipertensión, el cáncer y la artritis reumatoide. Debido a que son parte de los programas específicos de lo sistema de salud, la dificultad de acceso a estos fármacos y la consiguiente judicialización de la salud de manifiesto la fragilidad de las políticas públicas existentes. Conclusiones Por último, está la conclusión de que los tribunales, a pesar de ser una estrategia para garantizar el acceso a la medicina, presenta incapacidad para hacer frente al juicio de las acciones y por lo tanto genera distorsiones en el flujo de los sistemas públicos.
Descritores: Ácido Aspártico Endopeptidases/genética
Cacau/enzimologia
Leucina/análogos & derivados
Sementes/enzimologia
-Sequência de Aminoácidos
Ácido Aspártico Endopeptidases/efeitos dos fármacos
Ácido Aspártico Endopeptidases/metabolismo
Clonagem Molecular
Cacau/genética
Cumarínicos/farmacologia
DNA Complementar/química
DNA Complementar/genética
DNA Complementar/isolamento & purificação
Regulação Enzimológica da Expressão Gênica
Regulação da Expressão Gênica de Plantas
Concentração de Íons de Hidrogênio
Isoenzimas/efeitos dos fármacos
Isoenzimas/genética
Isoenzimas/metabolismo
Leucina/farmacologia
Dados de Sequência Molecular
Filogenia
Pepstatinas/farmacologia
Proteínas de Plantas/genética
Proteínas de Plantas/metabolismo
RNA Mensageiro/genética
RNA Mensageiro/metabolismo
Análise de Sequência de DNA
Homologia de Sequência de Aminoácidos
Sementes/genética
Yarrowia/genética
Yarrowia/metabolismo
Responsável: CO332 - Facultad de Medicina


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Masuda, Aoi
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Id: lil-606757
Autor: Parizi, Luís Fernando; Pohl, Paula Cristiane; Masuda, Aoi; Vaz Junior, Itabajara da Silva.
Título: New approaches toward anti-Rhipicephalus (Boophilus) microplus tick vaccine / Novas estratégias para o desenvolvimento de uma vacina contra o carrapato Rhipicephalus (Boophilus) microplus
Fonte: Rev. bras. parasitol. vet;18(1):1-7, Mar. 2009.
Idioma: en.
Resumo: The tick Rhipicephalus (Boophilus) microplus (formerly Boophilus microplus) is the major ectoparasite affecting livestock in America, Asia, Africa, and Oceania. Conventional tick control is based on the use of acaricides but immunization of bovines with tick gut proteins induces only a partial protective immune response. Based on this information, distinct research groups have explored the possibility of protecting the animals by inducing an immune response against other tick proteins. However, the antigens so far described do not induce the necessary protection for suppressing the use of acaricides. Currently, several groups are engaged in identifying new tick proteins to be used as targets for the development of new vaccines. This approach focuses on the enhancement of the immunogenicity of antigens already tested by incorporating new adjuvants or formulations and by searching for new antigens. This paper reviews the work done by Brazilian researchers to develop a vaccine against this tick.

O carrapato Rhipicephalus (Boophilus) microplus (anteriormente Boophilus microplus) é o principal ectoparasita que afeta bovinos na América, Ásia, África e Oceania e o seu controle é tradicionalmente realizado através do uso de acaricidas. Experimentos de imunização com proteínas do carrapato mostram que a resposta imune desenvolvida pelos bovinos vacinados protege, em parte, os animais do parasitismo. Baseado nessas observações, vários grupos de pesquisa exploram a possibilidade de proteger os animais pela indução de uma resposta imune contra proteínas do carrapato. Entretanto, os antígenos já caracterizados não asseguram o grau de proteção necessário para suprimir o uso de acaricidas. Portanto, esses grupos de pesquisa estão engajados na tentativa de identificar novas proteínas que possam ser utilizadas para o desenvolvimento de novas vacinas, as quais possam induzir maior imunogenicidade de que os antígenos já testados, através do uso de novas formulações e/ou pela incorporação de adjuvantes. O presente artigo apresenta uma revisão da literatura sobre os resultados obtidos por pesquisadores brasileiros no desenvolvimento de vacinas contra o carrapato.
Descritores: Rhipicephalus
Infestações por Carrapato/prevenção & controle
Vacinas
-Ácido Aspártico Endopeptidases
Precursores Enzimáticos
Serina Endopeptidases
Limites: Animais
Tipo de Publ: Research Support, Non-U.S. Gov't
Revisão
Responsável: BR1.1 - BIREME


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Id: lil-540281
Autor: Mejía, John Santiago; Hong, Xiquian; Carlow, Clotilde K. S..
Título: Presencia de un dominio tipo mucina en una familia de glicoproteinas inmunodominantes de filarias que son homólogas al inhibidor de aspartil-proteinasas de Ascaris Suum / Mucin-type domain presence in an immunedomaining glycoprotein family of filarias that are homologues to the aspartil-proteinases of Ascaris suum inhibitor
Fonte: CES med;10(1):34-36, ene.-jun. 1996. tab, graf.
Idioma: es.
Resumo: El análisis de la secuencia primaria de un antígeno inmunodominante de Dirofilaria immitis (DiT33) permitió identificar un dominio tipo mucina en el segmento carboxilo-terminal de la proteína. Los residuos de serina y treonina en este segmento permite el anclaje por puentes del tipo O de varios glicanos (T-fil) similares al antígeno T (Thomsen-Friedenreich) que se expresa en adenocarcinomas. El glicano T-fil se expresa ampliamente en diversos estadíos de diferenciación de D. immitis y posiblemente representa el principal glicano usado por las filarias para formar mucinas...
Descritores: Ascaris suum
Ácido Aspártico Endopeptidases
Mucinas
Subfamília B de Transportador de Cassetes de Ligação de ATP
Polissacarídeos
-Aminoácidos
Dirofilaria immitis
Tipo de Publ: Relatório Técnico
Responsável: CO83.1 - Biblioteca Fundadores


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Id: lil-520787
Autor: Braga-Silva, L. A; Mesquita, D. G. A; Ribeiro, M. D; Carvalho, S. M. F; Fracalanzza, S. E. L; Santos, A. L. S.
Título: Trailing end-point phenotype antibiotic-sensitive strains of Candida albicans produce different amounts of aspartyl peptidases
Fonte: Braz. j. med. biol. res = Rev. bras. pesqui. méd. biol;42(8):765-770, Aug. 2009. ilus, tab, graf.
Idioma: en.
Resumo: Candida albicans is an opportunistic fungal pathogen that causes severe systemic infections in immunosuppressed individuals. C. albicans resistance to antifungal drugs is a severe problem in patients receiving prolonged therapy. Moreover, trailing yeast growth, which is defined as a resistant MIC after 48 h of incubation with triazole antifungal agents but a susceptible MIC after 24 h, has been noted in tests of antifungal susceptibility against some C. albicans isolates. In this context, we recently noticed this phenomenon in our routine susceptibility tests with fluconazole/itraconazole and C. albicans clinical isolates. In the present study, we investigated the production of cell-associated and secreted aspartyl peptidases (Saps) in six trailing clinical isolates of C. albicans, since this class of hydrolytic enzymes is a well-known virulence factor expressed by this fungal pathogen. Sap2, which is the best-studied member of the Sap family, was detected by flow cytometry on the cell surface of yeasts and as a 43-kDa polypeptide in the culture supernatant, as demonstrated by Western blotting assay using an anti-Sap1-3 polyclonal antibody. Released aspartyl peptidase activity was measured with BSA hydrolysis and inhibited by pepstatin A, showing distinct amounts of proteolytic activity ranging from 5.7 (strain 44B) to 133.2 (strain 11) arbitrary units. Taken together, our results showed that trailing clinical isolates of C. albicans produced different amounts of both cellular and secreted aspartyl-type peptidases, suggesting that this phenotypic feature did not generate a regular pattern regarding the expression of Sap.
Descritores: Antifúngicos/farmacologia
Ácido Aspártico Endopeptidases/análise
Candida albicans/enzimologia
Fluconazol/farmacologia
Itraconazol/farmacologia
-Ácido Aspártico Endopeptidases/efeitos dos fármacos
Candida albicans/efeitos dos fármacos
Candida albicans/crescimento & desenvolvimento
Testes de Sensibilidade Microbiana
Fenótipo
Soroalbumina Bovina
Limites: Adulto
Idoso
Pré-Escolar
Feminino
Humanos
Masculino
Pessoa de Meia-Idade
Tipo de Publ: Relatos de Casos
Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


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Id: lil-517023
Autor: Parra-Ortega, B; Cruz-Torres, H; Villa-Tanaca, L; Hernández-Rodríguez, C.
Título: Phylogeny and evolution of the aspartyl protease family from clinically relevant Candida species
Fonte: Mem. Inst. Oswaldo Cruz;104(3):505-512, May 2009. ilus.
Idioma: en.
Projeto: CONACyT; . IPN. SIP.
Resumo: Aspartyl proteases are a class of enzymes that include the yeast aspartyl proteases and secreted aspartyl protease (Sap) superfamilies. Several Sap superfamily members have been demonstrated or suggested as virulence factors in opportunistic pathogens of the genus Candida. Candida albicans, Candida tropicalis, Candida dubliniensis and Candida parapsilosis harbour 10, four, eight and three SAP genes, respectively. In this work, genome mining and phylogenetic analyses revealed the presence of new members of the Sap superfamily in C. tropicalis (8), Candida guilliermondii (8), C. parapsilosis(11) and Candida lusitaniae (3). A total of 12 Sap families, containing proteins with at least 50 percent similarity, were discovered in opportunistic, pathogenic Candida spp. In several Sap families, at least two subfamilies or orthologous groups were identified, each defined by > 90 percent sequence similitude, functional similarity and synteny among its members. No new members of previously described Sap families were found in a Candida spp. clinical strain collection; however, the universality of SAPT gene distribution among C. tropicalis strains was demonstrated. In addition, several features of opportunistic pathogenic Candida species, such as gene duplications and inversions, similitude, synteny, putative transcription factor binding sites and genome traits of SAP gene superfamily were described in a molecular evolutionary context.
Descritores: Ácido Aspártico Endopeptidases/genética
Candida/enzimologia
Evolução Molecular
Filogenia
-Candida/classificação
Candida/genética
DNA Fúngico/genética
Genes Fúngicos
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


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Id: lil-482088
Autor: Melo, A. S; Padovan, A. C; Serafim, R. C; Puzer, L; Carmona, A. K; Juliano Neto, L; Brunstein, A; Briones, M. R.
Título: The Candida albicans AAA ATPase homologue of Saccharomyces cerevisiae Rix7p (YLL034c) is essential for proper morphology, biofilm formation and activity of secreted aspartyl proteinases
Fonte: Genet. mol. res. (Online);5(4):664-687, 2006. graf, ilus.
Idioma: en.
Resumo: Proper morphology is essential for the ability of Candida albicans to switch between yeast and hyphae and thereby sustain its virulence. Here we identified, by differential screening, a novel C. albicans AAA ATPase encoding gene, CaYLL34 (RIX7), with enhanced expression in hyphae. Phylogenetic analysis suggests that CaYLL34 belongs to a [quot ]VCP-like[quot ] subgroup of AAA ATPases essential for yeast viability and contains a bipartite nuclear localization signal. Inactivation of one copy of CaYLL34, by the URA-Blaster method, generated the heterozygous mutant strain M61. This strain has severe phenotypic alterations, such as a highly increased vacuole, abnormal cell shape and reduced growth in different conditions. Also, major pathogenicity factors are affected in M61, for instance, a significant decrease of hypha formation (>90%), surface biofilm adhesion (86%) and secreted aspartyl proteinase activity (76.5%). Our results show that the partial impairment of CaYll34p cellular levels is sufficient to affect the proper cellular morphology and pathogenicity factors and suggest that this protein is required for biogenesis of ribosomal subunits. Accordingly, we propose that the product of CaYLL34 could be tested as a novel target for antifungal drugs.
Descritores: Adenosina Trifosfatases/genética
Biofilmes/crescimento & desenvolvimento
Candida albicans/genética
Ácido Aspártico Endopeptidases/metabolismo
Proteínas de Saccharomyces cerevisiae/genética
-Sequência de Bases
Candida albicans/enzimologia
Candida albicans/crescimento & desenvolvimento
Hifas/enzimologia
Hifas/genética
Hifas/crescimento & desenvolvimento
Dados de Sequência Molecular
Mutação
Filogenia
Reação em Cadeia da Polimerase Via Transcriptase Reversa
Análise de Sequência de DNA
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


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Id: lil-472532
Autor: Mardegan, Rita de Cássia; Foglio, Mary Ann; Gonçalves, Reginaldo Bruno; Hõfling, José Francisco.
Título: Candida albicans proteinases
Fonte: Braz. j. oral sci;5(16):944-952, 2006. ilus.
Idioma: en.
Resumo: Candida species are ubiquitous commensal yeast that usually reside as part of an individual´s normal mucosal microflora and can be detected in approximately 50% of the population in this form. However, if the balance of the normal flora is disrupted or the immune defences are compromised, Candida species can invade mucosal surfaces and cause disease manifestations. Determining exactly how this transformation from commensal to pathogen takes place and how it can be prevented is a continuing challenger for the medical mycology field. Attributes that contribute to Candida albicans virulence include adhesion, hyphal formation, phenotypic switching and extra cellular hydrolytic enzyme production. The extra cellular hydrolytic enzyme, especially the secreted aspartyl proteinases (Saps), are one a few gene products that have been shown to directly contribute to C. albicans pathogenicity. Given the limited number of suitable and effective antifungal drugs, the continuing increase in the incidence of Candida infections, together with increasing drug resistance, highlights the need to discover new and better agents that target fundamental biological processes and or pathogenic determinants of C. albicans.
Descritores: Candida albicans
Peptídeo Hidrolases
Fatores de Virulência
-Candida
Ácido Aspártico Endopeptidases
Inibidores de Proteases
Tipo de Publ: Revisão
Responsável: BR28.1 - Serviço de Biblioteca e Documentação Professor Doutor Antônio Gabriel Atta


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Id: lil-440638
Autor: Koehler, Jeffrey W; Morales, Maria E; Shelby, Bryan D; Brindley, Paul J.
Título: Aspartic protease activities of schistosomes cleave mammalian hemoglobins in a host-specific manner
Fonte: Mem. Inst. Oswaldo Cruz;102(1):83-85, Feb. 2007. ilus.
Idioma: en.
Resumo: We examined the efficiency of digestion of hemoglobin from four mammalian species, human, cow, sheep, and horse by acidic extracts of mixed sex adults of Schistosoma japonicum and S. mansoni. Activity ascribable to aspartic protease(s) from S. japonicum and S. mansoni cleaved human hemoglobin. In addition, aspartic protease activities from S. japonicum cleaved hemoglobin from bovine, sheep, and horse blood more efficiently than did the activity from extracts of S. mansoni. These findings support the hypothesis that substrate specificity of hemoglobin-degrading proteases employed by blood feeding helminth parasites influences parasite host species range; differences in amino acid sequences in key sites of the parasite proteases interact less or more efficiently with the hemoglobins of permissive or non-permissive hosts.
Descritores: Ácido Aspártico Endopeptidases/metabolismo
Hemoglobinas/metabolismo
Schistosoma japonicum/enzimologia
Schistosoma mansoni/enzimologia
-Cavalos
Interações Hospedeiro-Parasita
Hidrólise
Ovinos
Especificidade por Substrato
Limites: Humanos
Animais
Bovinos
Camundongos
Responsável: BR1.1 - BIREME



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