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Id: biblio-1151755
Autor: Dusse, Luci Maria Sant'Ana; Rios, Danyelle Romana Alves; Sousa, Letícia Parreiras Nunes; Moraes, Rívia Mara Morais e Silva; Domingueti, Caroline Pereira; Gomes, Karina Braga Gomes.
Título: Biomarcadores da função renal: do que dispomos atualmente? / Biomarkers of renal function: what is currently available?
Fonte: Rev. bras. anal. clin;49(1):41-51, jun.16, 2017.
Idioma: pt.
Resumo: A avaliação da função renal é de extrema importância na prática clínica, tanto para o diagnóstico quanto para e prognóstico e monitoração das doenças renais. Neste contexto, aparticipação do laboratório é de grande importância, uma vez que a maior parte das doenças renais só se manifesta clinicamente quando mais de 50% a 75% da função renal estácomprometida. O desenvolvimento de novos biomarcadores para diagnóstico precoce, estratificação de risco, prognóstico de lesão renal tem sido um dos principais alvos das pesquisas envolvendo o sistema renal. Dessa forma, diversos novos biomarcadores, tais como lipocalina associada à gelatinase de neutrófilos (NGAL), cistatina C, molécula-1 de lesão renal (KIM-1), interleucina-18 (IL-18), enzimas urinárias tubulares e proteínas de baixo peso molecular, dentre outros, têm sido propostos para diagnosticar /monitorar as doenças renais agudas e crônicas. Este estudo visa discutir aspectos associados aos principais biomarcadores utilizados na rotina laboratorial para diagnóstico, prognóstico e acompanhamento do paciente com disfunção renal, bem como apresentar novos marcadores que se destacam na literatura recente e que podem ser promissores na prática clínica

The assessment of renal function is very important in clinical practice, both for diagnosis and for prognosis and monitoring of renal diseases. In this context, the role of the laboratory is of great importance, since most of the kidney disease manifests itself clinically only when more than 50 to 75% of kidney function is compromised. The development of new biomarkers for early diagnosis, risk stratification, prognosis of renal injury has been a major focus of research involving the renal system. Thus, several new biomarkers, such as neutrophil gelatinase-associated lipocalin (NGAL), kidney injury molecule-1 (KIM-1), interleukin-18 (IL18) and low-molecular weight proteins and enzymes, and others, have been proposed to diagnose/monitoring acute and chronic renal diseases. The aim of this study is to discuss aspects related to the main biomarkers used in routine laboratory tests for diagnosis, prognosis and monitoring of patients with renal dysfunction, as well as provide new markers that stand out in the recent literature, and that may be promising in clinical practice
Descritores: Técnicas de Laboratório Clínico
Insuficiência Renal
Testes Laboratoriais
Lesão Renal Aguda
Falência Renal Crônica
-Proteinúria
Ureia
Biomarcadores
Quelantes de Ferro
Gelatinases
Interleucina-18
Creatinina
Albuminúria
Lipocalinas
Cistatina C
Inulina
Testes de Função Renal
Responsável: BR408.1 - Biblioteca da Faculdade de Medicina - BFM


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Id: biblio-835634
Autor: Lago, Matheus Wagner; Moresco, Rafael Noal; Bochi, Guilherme Vargas.
Título: Lipocalina associada à gelatinase neutrofílica(NGAL) como um biomarcador de lesão renal: uma revisão / Neutrophil gelatinase-associated lipocalin (NGAL) as a biomarker of kidney damage: a review
Fonte: Rev. Inst. Adolfo Lutz;75(único):1/13-13/13, 2016. tab.
Idioma: pt.
Resumo: Lipocalina associada à gelatinase neutrofílica (NGAL) é uma molécula proteica predominantemente expressa na parte distal do néfron após a ocorrência de lesão renal. Diferentemente da creatinina sérica e da taxa de filtração glomerular, que são marcadores de função renal, os níveis aumentados de NGAL, tanto no soro quanto na urina, estão intimamente ligados a lesões estruturais do néfron.Os estudos clínicos indicam que poucas horas após a ocorrência da lesão renal aguda os níveis séricos e urinários de NGAL já se apresentam significativamente elevados, enquanto os níveis séricos de creatinina e a sua depuração renal apenas sofrem alterações significativas entre 24-48hapós a lesão. Assim, a utilização de marcadores de função renal, usualmente avaliados na práticaclínica, pode apresentar algumas limitações além de dificultar a aplicação de medidas precoces quevisam a proteção renal. Esta revisão da literatura tem por objetivo analisar os aspectos biológicos e as aplicações da mensuração de NGAL em algumas condições clínicas, incluindo injúria renal, nefropatias e isquemia renal.

Neutrophil gelatinase-associated lipocalin (NGAL) is a protein molecule predominantly expressed in the distal part of the nephron following the occurrence of renal damage. Differently from serum creatinine and glomerular filtration rate, which are markers of renal function, increased levels of NGAL in both serum and urine are closely linked to structural damage of the nephron. Clinical studies indicate that within a few hours of occurrence Of acute renal injury serum and urinary NGAL levels are already significantly elevated, while serum creatinine levels and renal clearance only change significantly between 24-48 hours after injury. Thus, the use of renal function markers, usually evaluated in practice, may present some limitations, as well as hinder the application of early measures that aim at renal protection. This review of the literature aims to analyze the biological aspects and applications of NGAL measurement in some clinical conditions, including renal injury, nephropathy and renal ischemia.
Descritores: Biomarcadores Farmacológicos
Gelatinases
Lesão Renal Aguda
Lipocalinas
Responsável: BR91.2 - Centro de Documentação


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Id: biblio-1018169
Autor: Cabrera Coronel, Amapola Mónica,.
Título: Proyento industrial; Gelatina, comestible a partir de desechos de curtiembre.
Fonte: San Lorenzo; s.n; nov.1992. 80 p.
Idioma: es; en.
Tese: Apresentada a Facultad de Ciencias Quimicas para obtenção do grau de Tesis.
Resumo: Trata sobre elaboración de gelatina comestible a partir de desechos de curtiembres, es un tipo de anómalo de coloide, en el cual un fino reticulo se extiende por el seno de un liquido se presentara en forma de polvo fino de dos maneras cuyo color dependera de los aditivos
Descritores: Gelatinases
-Gelatina
Responsável: PY37.1 - Biblioteca
66426#C111p


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Texto completo SciELO Brasil
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Id: lil-783800
Autor: Girgin, Mustafa; Binnetoglu, Kenan; Duman, Kazim; Kanat, Burhan Hakan; Cetinkaya, Ziya; Ayten, Refik; Ilhan, Yavuz Selim; Ilhan, Necip; Seker, Ibrahim; Timurkaan, Necati.
Título: Effects of platelet rich plasma on fascial healing in rats with fecal peritonitis
Fonte: Acta cir. bras;31(5):314-319, May 2016. tab, graf.
Idioma: en.
Resumo: ABSTRACT PURPOSE : To evaluate the effects of platelet rich plasma (PRP) on the healing of fascia wherein peritonitis has been created. METHODS: Twenty eight Wistar Albino rats were divided into four groups. Only a primary fascial repair following laparotomy was performed on Group 1, a primary fascial repair performed and PRP treatment applied following laparotomy on Group 2, and a fecal peritonitis created following laparotomy and a primary fascial repair carried out on Group 3. A fecal peritonitis was created following laparotomy and primary fascial repair and PRP treatment on the fascia was carried out on Group 4. RESULTS: TNF-α was found to be significantly lower in the control group (Group 1). It was detected at the highest level in the group in which fecal peritonitis was created and PRP applied (Group 4). TGF-β was determined as being significantly higher only in Group 4. Histopathologically, the differences between the groups in terms of cell infiltration and collagen deposition were not found to be significant. CONCLUSION: When platelet rich plasma was given histologically and biochemicaly as wound healing parameters cellular infiltration, collagen accumulation, and tissue hydroxyiproline levels were not increased but neovascularization, fibroblast activation and TNF Alfa levels were increased and PRP accelerated wound healing.
Descritores: Peritonite/complicações
Cicatrização
Plasma Rico em Plaquetas
Fáscia/fisiologia
-Peritonite/metabolismo
Serina Endopeptidases/metabolismo
Distribuição Aleatória
Fator de Crescimento Transformador beta/análise
Fator de Crescimento Transformador beta/metabolismo
Colágeno/efeitos dos fármacos
Colágeno/metabolismo
Fator de Necrose Tumoral alfa/análise
Fator de Necrose Tumoral alfa/metabolismo
Ratos Wistar
Gelatinases/metabolismo
Neovascularização Fisiológica
Modelos Animais
Fáscia/irrigação sanguínea
Hidroxiprolina/análise
Hidroxiprolina/metabolismo
Proteínas de Membrana/metabolismo
Limites: Animais
Responsável: BR1.1 - BIREME


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Id: biblio-864253
Autor: Hannas, Angélica Reis.
Título: Determinação da expressão de MMP-2 e MMP-9 na saliva de pacientes portadores de lesões cervicais não cariosas e da influência das MMPs sobre lesões radiculares artificiais através de EDX / Gelatinase expression in saliva of patients with noncarious cervical lesions and EDX assessment of the influence of matrix metalloproteinases on artificial root lesions.
Fonte: Bauru; s.n; 2007. 161 p.
Idioma: pt.
Tese: Apresentada a Universidade de São Paulo. Faculdade de Odontologia de Bauru para obtenção do grau de Doutor.
Descritores: Gelatinases
Líquido do Sulco Gengival
Metaloproteinases da Matriz
Cárie Radicular
Saliva
Inibidores Teciduais de Metaloproteinases
-Remineralização Dentária
Responsável: BR28.1 - Serviço de Biblioteca e Documentação Professor Doutor Antônio Gabriel Atta
BR28.1; H191d


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Texto completo SciELO Brasil
Rall, Vera Lúcia Mores
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Id: lil-709493
Autor: Camargo, Carlos Henrique; Bruder-Nascimento, Ariane; Lee, Sarah Hwa In; Fernandes Júnior, Ary; Kaneno, Ramon; Rall, Vera Lúcia Mores.
Título: Prevalence and phenotypic characterization of Enterococcus spp. isolated from food in Brazil
Fonte: Braz. j. microbiol;45(1):111-115, 2014. tab.
Idioma: en.
Resumo: We evaluated the frequency of enterococci from food and found 95.2% of positivity, being E. faecium and E. faecalis the most frequent species. High-level streptomycin resistance was observed, as well as gelatinase and hemolysis activity, showing the potential role of environmental strains as reservoir of virulence and resistance traits.
Descritores: Enterococcus/classificação
Enterococcus/isolamento & purificação
Contaminação de Alimentos
Microbiologia de Alimentos
-Brasil
Farmacorresistência Bacteriana
Enterococcus/fisiologia
Gelatinases/análise
Hemólise
Prevalência
Fatores de Virulência/análise
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


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Id: lil-709469
Autor: Medeiros, A.W.; Pereira, R.I.; Oliveira, D.V.; Martins, P.D.; d'Azevedo, P.A.; Van der Sand, S.; Frazzon, J.; Frazzon, A.P.G.
Título: Molecular detection of virulence factors among food and clinical Enterococcus faecalis strains in South Brazil
Fonte: Braz. j. microbiol;45(1):327-332, 2014.
Idioma: en.
Projeto: Conselho Nacional de Desenvolvimento Científico e Tecnológico.
Resumo: The present report aimed to perform a molecular epidemiological survey by investigating the presence of virulence factors in E. faecalis isolated from different human clinical (n = 57) and food samples (n = 55) in Porto Alegre, Brazil, collected from 2006 to 2009. In addition, the ability to form biofilm in vitro on polystyrene and the β-haemolytic and gelatinase activities were determined. Clinical strains presented a higher prevalence of aggregation substance (agg), enterococcal surface protein (esp) and cytolysin (cylA) genes when compared with food isolates. The esp gene was found only in clinical strains. On the other hand, the gelatinase (gelE) and adherence factor (ace) genes had similar prevalence among the strains, showing the widespread occurrence of these virulence factors among food and clinical E. faecalis strains in South Brazil. More than three virulence factor genes were detected in 77.2% and 18.2% of clinical and food strains, respectively. Gelatinase and β-haemolysin activities were not associated with the presence of gelE and cylA genes. The ability to produce biofilm was detected in 100% of clinical and 94.6% of food isolates, and clinical strains were more able to form biofilm than the food isolates (Student's t-test, p < 0.01). Results from the statistical analysis showed significant associations between strong biofilm formation and ace (p = 0.015) and gelE (p = 0.007) genes in clinical strains. In conclusion, our data indicate that E. faecalis strains isolated from clinical and food samples possess distinctive patterns of virulence factors, with a larger number of genes that encode virulence factors detected in clinical strains.
Descritores: Proteínas de Bactérias/genética
Enterococcus faecalis/genética
Microbiologia de Alimentos
Infecções por Bactérias Gram-Positivas/microbiologia
Fatores de Virulência/genética
-Brasil
Biofilmes/crescimento & desenvolvimento
Enterococcus faecalis/isolamento & purificação
Enterococcus faecalis/fisiologia
Gelatinases/análise
Hemólise
Limites: Humanos
Tipo de Publ: Estudo Comparativo
Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


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Id: lil-680773
Autor: Memorias do Instituto Oswaldo Cruz; Comerlato, Carolina Baldisserotto; Resende, Mariah Costa Carvalho de; Caierao, Juliana; d'Azevedo, Pedro Alves.
Título: Presence of virulence factors in Enterococcus faecalis and Enterococcus faecium susceptible and resistant to vancomycin
Fonte: Mem. Inst. Oswaldo Cruz;108(5):590-595, ago. 2013. tab.
Idioma: en.
Projeto: FAPERGS.
Resumo: Despite the increasing importance of Enterococcus as opportunistic pathogens, their virulence factors are still poorly understood. This study determines the frequency of virulence factors in clinical and commensal Enterococcus isolates from inpatients in Porto Alegre, Brazil. Fifty Enterococcus isolates were analysed and the presence of the gelE, asa1 and esp genes was determined. Gelatinase activity and biofilm formation were also tested. The clonal relationships among the isolates were evaluated using pulsed-field gel electrophoresis. The asa1, gelE and esp genes were identified in 38%, 60% and 76% of all isolates, respectively. The first two genes were more prevalent in Enterococcus faecalis than in Enterococcus faecium, as was biofilm formation, which was associated with gelE and asa1 genes, but not with the esp gene. The presence of gelE and the activity of gelatinase were not fully concordant. No relationship was observed among any virulence factors and specific subclones of E. faecalis or E. faecium resistant to vancomycin. In conclusion, E. faecalis and E. faecium isolates showed significantly different patterns of virulence determinants. Neither the source of isolation nor the clonal relationship or vancomycin resistance influenced their distribution.
Descritores: Antibacterianos/farmacologia
Enterococcus faecalis
Enterococcus faecium
Vancomicina/farmacologia
Fatores de Virulência/genética
-Biofilmes/crescimento & desenvolvimento
Eletroforese em Gel de Campo Pulsado
Enterococcus faecalis/efeitos dos fármacos
Enterococcus faecalis/enzimologia
Enterococcus faecalis/patogenicidade
Enterococcus faecium/efeitos dos fármacos
Enterococcus faecium/enzimologia
Enterococcus faecium/patogenicidade
Gelatinases/metabolismo
Testes de Sensibilidade Microbiana
Resistência a Vancomicina/genética
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


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Id: lil-667933
Autor: Lisboa, Rodolfo Assis; Andrade, Marcus Vinícius; Cunha-Melo, José Renan.
Título: Zimography is an effective method for detection of matrix metalloproteinase 2 (MMP-2) activity in cultured human fibroblasts
Fonte: Acta cir. bras;28(3):216-220, Mar. 2013. ilus.
Idioma: en.
Resumo: PURPOSE: To describe a method to characterize the gelatinase activity of cultured human periodontal fibroblasts stimulated with Pam3Cys and E. coli LPS, ligands of TLR2 and TLR4 respectively, and by centrifugation of the cultures, simulating an orthodontic force. METHODS: To study MMP-2 activity, primary cultures of human periodontal fibroblasts were stimulated with the addition of TLRs 2 and 4 ligands and the application of mechanical force by centrifugation at 141 x g for 30 min. Supernatant media was collected 24 hours later to perform protein quantification and zymography. RESULTS: MMP-2 activity suffered an increase in cultures co-stimulated with TLRs 2 and 4 ligands alone or with the presence of mechanical force application compared to basal levels. CONCLUSION: Zymography, one of the several methods to study MMPs activities, is a simple, qualitative and efficient method based on electrophoresis of bis-acrylamide gels copolymerized with a protein substrate.
Descritores: Eletroforese/métodos
Fibroblastos/enzimologia
/análise
MATRIX METALLOPROTEINASE TEMEFOS/análise
-Sobrevivência Celular
Células Cultivadas
Gelatinases/fisiologia
Lipoproteínas
/fisiologia
MATRIX METALLOPROTEINASE TEMEFOS/fisiologia
Ligamento Periodontal/citologia
Reprodutibilidade dos Testes
Estatísticas não Paramétricas
Fatores de Tempo
Receptores Toll-Like/análise
Limites: Humanos
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


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Id: lil-665850
Autor: Lima e Silva, Agostinho A de; Carvalho, Márcia A Ribeiro de; Souza, Sérgio A L de; Dias, Patrícia M Teixeira; Silva Filho, Renato G da; Saramago, Carmen S de Meirelles; Bento, Cleonice A de Melo; Hofer, Ernesto.
Título: Heavy metal tolerance (Cr, Ag and Hg) in bacteria isolated from sewage
Fonte: Braz. j. microbiol;43(4):1620-1631, Oct.-Dec. 2012. ilus, tab.
Idioma: en.
Resumo: Samples of sewage from a university hospital and a chemistry technical school were analysed for the percentage of bacterial tolerance to chromium (Cr), silver (Ag) and mercury (Hg). Additionally, we investigated the effect of these metals on pigmentation and on some enzymatic activities of the metal tolerant strains isolated, as well as antimicrobial resistance in some metal tolerant Enterobacteriaceae strains. Tolerance to Cr was observed mainly in Gram positive bacteria while in the case of Ag and Hg the tolerant bacteria were predominately Gram negative. Hg was the metal for which the percentage of tolerance was significantly higher, especially in samples from the hospital sewage (4.1%). Mercury also had the most discernible effect on color of the colonies. Considering the effect of metals on the respiratory enzymes, one strain of Ag-tolerantBacillus sp. and one of Hg-tolerant P. aeruginosa were unable to produce oxidase in the presence of Ag and Hg, respectively, while the expression of gelatinase was largely inhibited in various Gram negative strains (66% by Cr). Drug resistance in Hg-tolerant Enterobacteriaceae strains isolated from the university hospital sewage was greater than 80%, with prevalence of multiple resistance, while the Ag-tolerant strains from the same source showed about 34% of resistance, with the predominance of mono-resistance. Our results showed that, despite the ability of metal tolerant strains to survive and grow in the presence of these elements, the interactions with these metals may result in metabolic or phisiological changes in this group of bacteria.
Descritores: Águas Residuárias/análise
Resistência a Medicamentos
Infecções por Enterobacteriaceae
Enterobacteriaceae/isolamento & purificação
Enterobacteriaceae/metabolismo
Gelatinases/análise
Metais Pesados/isolamento & purificação
Metais Pesados/metabolismo
Oxirredutases/análise
Pigmentos Biológicos/análise
-Ativação Enzimática
Hospitais
Prevalência
Amostras de Água
Limites: Humanos
Tipo de Publ: Estudo Comparativo
Responsável: BR32.1 - Serviço de Biblioteca e Informação Biomédica



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