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Pesquisa : D08.811.277.656.300.760.875 [Categoria DeCS]
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Id: biblio-887383
Autor: Ozdemir, Abdurrahman A.
Título: Activador tisular del plasminógeno recombinante para el tratamiento de la vegetación intracardíaca en un lactante de muy bajo peso al nacer / Recombinant tissue plasminogen activator treatment for intracardiac vegetation in a very low birth weight infant
Fonte: Arch. argent. pediatr;115(5):307-310, oct. 2017. ilus.
Idioma: en; es.
Resumo: Las nuevas opciones de tratamiento prolongan la hospitalización y aumentan las infecciones intrahospitalarias bacterianas y fúngicas, pero también mejoran la sobrevida de los recién nacidos hospitalizados en la unidad de cuidados intensivos neonatales. Las infecciones fúngicas invasivas en neonatos están asociadas con una morbimortalidad significativa. También pueden diseminarse a órganos específicos y causar endocarditis, endoftalmitis, artritis séptica, nefropatía obstructiva y meningitis. En el caso de la endocarditis, se recomiendan tratamientos antimicóticos sistémicos agresivos y, en algunos casos, la intervención quirúrgica del neonato. Informamos el caso de un lactante prematuro, de bajo peso al nacer, con vegetación intracardíaca. Esta es una complicación rara y potencialmente mortal de infecciones fúngicas invasivas. El paciente recibió tratamiento con caspofungina y un activador tisular del plasminógeno recombinante, en vez de una intervención quirúrgica.

Developing treatment options have resulted in prolonged admission and increased bacterial and fungal nosocomial infections as well as improved survival in neonatal intensive care unit. Invasive fungal infections in newborns are associated with significant morbidity and mortality and can cause endorgan dissemination such as endocarditis, endophthalmitis, septic arthritis, obstructive nephropathy and meningitis. Endocarditis requires aggressive systemic antifungal therapy and sometimes surgical intervention in neonates. We report a low birth weight premature infant with intracardiac vegetation that is rare and a life-threatening complication of invasive fungal infections. He was treated with caspofungin and recombinant tissue plasminogen activator in stead of surgical intervention.
Descritores: Candidíase/tratamento farmacológico
Ativador de Plasminogênio Tecidual
Endocardite/microbiologia
Endocardite/tratamento farmacológico
Equinocandinas/uso terapêutico
Lipopeptídeos/uso terapêutico
Candida parapsilosis
Antifúngicos/uso terapêutico
-Proteínas Recombinantes/uso terapêutico
Recém-Nascido de muito Baixo Peso
FRONTAL SINUS0
Limites: Seres Humanos
Masculino
Recém-Nascido
Tipo de Publ: Relatos de Casos
Responsável: AR94.1 - Centro de Información Pediatrica


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Id: biblio-1010296
Autor: Avello, Veronica; Tapia, Bethzabeth; Vergara, Mauricio; Acevedo, Cristian; Berrios, Julio; Reyes, Juan G; Altamirano, Claudia.
Título: Impact of sodium butyrate and mild hypothermia on metabolic and physiological behaviour of CHO TF 70R cells
Fonte: Electron. j. biotechnol;27:55-62, May. 2017. tab, graf.
Idioma: en.
Projeto: FONDECYT; . Universidad Federico Santa Maria.
Resumo: Background: To reduce costs associated with productivity of recombinant proteins in the biopharmaceutical industry, research has been focused on regulatory principals of growth and survival during the production phases of the cell culture. The main strategies involve the regulation of cell proliferation by the modulation of cell cycle control points (G1/S or G2/M) with mild hypothermia and the addition of sodium butyrate (NaBu). In this study, batch culture strategies were evaluated using CHO TF 70R cells producing the recombinant human tissue plasminogen activator (rh-tPA), to observe their individual and combined effect on the cellular physiological state and relevant kinetic parameters. Results: NaBu addition has a negative effect on the mitochondrial membrane potential (ΔΨm), the values of which are remarkably diminished in cultures exposed to this cytotoxic compound. This effect was not reflected in a loss of cell viability. NaBu and mild hypothermic conditions increased the doubling time in the cell cultures, suggesting that these strategies triggered a general slowing of each cell cycle phase in a different way. Finally, the individual and combined effect of NaBu and mild hypothermia produced an increase in the specific rh-tPA productivity in comparison to the control at 37°C without NaBu. Nevertheless, both strategies did not have a synergistic effect on the specific productivity. Conclusions: The combination of NaBu addition and mild hypothermic condition causes an impact on physiological and metabolic state of CHO TF 70R cells, decreasing cell growth rate and improving glucose consumption efficiency. These results therefore provide a promising strategy to increase specific productivity of rh-tPA.
Descritores: Proteínas Recombinantes/metabolismo
Células CHO/metabolismo
Ativador de Plasminogênio Tecidual/metabolismo
Ácido Butírico/metabolismo
Hipotermia
-Ciclo Celular
Sobrevivência Celular
Células CHO/fisiologia
Ativador de Plasminogênio Tecidual/biossíntese
Proliferação Celular
Potencial da Membrana Mitocondrial
Responsável: CL1.1 - Biblioteca Central


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Id: biblio-1007039
Autor: Camargo Villarreal, Walter Mario; Urioste Avilés, María Teresa; Camargo Jordán, Walter Andrés; Ríos, Sandra; Montero, Jorge; Morales, Oscar.
Título: Trombolisis intravenosa en ataque cerebrovascular isquémico agudo en Santa Cruz Bolivia: análisis retrospectivo de los primeros 18 casos / Intravenous thrombolysis in acute ischemic stroke in Santa Cruz Bolivia: retrospective analysis of the first 18 cases
Fonte: Gac. méd. boliv;42(1):59-64, jun. 2019. ilus., tab..
Idioma: es.
Resumo: OBJETIVOS: describir la experiencia en la implementación del protocolo de trombolisis i.v. en 18 pacientes con Accidente Cerebrovascular (ACV) isquémico en la Unidad de ACV de la Clínica Foianini, concretamente, entre septiembre de 2013 y julio de 2017. MÉTODOS: el estudio tiene un enfoque cuantitativo, tipo descriptivo y retrospectivo. Se revisó las historias clínicas de los pacientes con diagnóstico de ACV isquémico agudo, que fueron tratados con trombolitico intravenoso (Alteplasa) en el servicio UTAC, entre septiembre de 2013 y julio 2017 en Santa Cruz, Bolivia. RESULTADOS: el Tiempo Síntoma-Puerta alcanzo a 62,7 +/- 38 min, mientras que el Tiempo Puerta-Aguja fue de 53,6 +/- 15 min. y Tiempo Síntoma-Aguja 114,6 +/- 43 min. Se registraron complicaciones en 6 (33,3%). promedio de 4,6 +/- 3 días de internación en la clínica, pasando un promedio de 2,8 +/- 2 días en la Unidad de ACV. La tasa de mortalidad fue de 16,6%. Según la Escala Modficada de Rankin, tuvieron un puntaje de 0-1 mRS. CONCLUSIONES: el tiempo Puerta-Aguja en nuestro establecimiento fue de un promedio de 57 minutos, cumpliendo de esta forma la recomendación de la American Heart Association Guidelines 2013, la cual recalca que éste debe ser <60 min13.

OBJECTIVE: describe the experience in the implementation of the thrombolysis intravenous protocol in eighteen patients with acute ischemic stroke in the Stroke unit from the Foianini Clinic specifically, between September of 2013 and July of 2017. METHODS: the study has a quantitative, descriptive and retrospective approach. The medical record of the patients with the acute ischemic stroke diagnosis treated with intravenous thrombolytic (Alteplasa) in the UTAC service between September of 2013 and July 2017 in Santa Cruz Bolivia was revised. RESULTS: symptoms-Door time reached to 62.7 +/- 38 minutes. While the time Door- needle time was 53.6 +/- 15 minutes and the time symptoms- needle 114.6 +/- 3 days of internment in the clinic, passing out an average of 2.8 +/- 2 days in the stroke unit. The mortality tax was 16.6%. According to the modified Rankin Scale, it had a 0-1 mRS score. CONCLUSIONS: the time Door-needle in our establishment was an average of 57 minutes, fulfilling in this way the recommendation of the American Heart Association Guidelines 2013, which emphasize that it has to be < 60 minutes.
Descritores: Terapia Trombolítica
-Ativador de Plasminogênio Tecidual/administração & dosagem
Acidente Vascular Cerebral/diagnóstico
Hipertensão
Limites: Seres Humanos
Tipo de Publ: Relatos de Casos
Responsável: BO138.1 - Biblioteca Central


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Id: biblio-997481
Autor: Castilla, Ruby C; Bromberger, Joyce T; Zhang, Yangang; Perel, James M; Matthews, Karen A.
Título: Depressive symptoms are related with hemostatic factors in middle-aged women: A report from the Study of Women Health Across the Nation (SWAN)
Fonte: MedUNAB;7(20):57-64, ago.-nov. 2004. ilus, tab.
Idioma: en.
Resumo: OBJECTIVE: Depression may be a risk factor for coronary heart disease (CHD) morbidity and mortality, but the mechanism(s) for the association are not established. The present study examined the relationship between one possible mechanism, hemostatic factors, and depressive symptoms in middle-aged women. METHOD: We measured levels of fibrinogen, Factor VIIc, plasminogen activator inhibitor antigen-1 (PAI-1), and tissue plasminogen activator antigen (TPA-ag) in 3,016 women aged 42-52 years enrolled in the Study of Women's Health Across the Nation (SWAN). Depressive symptoms were measured by the Center for Epidemiological Studies Depression Scale (CES-D), with scores > 16 suggestive of depression. RESULTS: Depressed women had high levels of all four hemostatic factors ( all p <0 .01). After controlling for age, smoking, ethnicity, prevalent cardiovascular disease, osteoarthritis, and dia-betes, and use of medications (including psychotropics), depressed women still had elevated levels of fibrinogen (mean, 95% confidence intervals 299, 304 ­ 295 mg/dl vs. 291, 294 ­ 288mg/dl, p= 0.003) and Factor VIIc (124, 127 ­ 121 ng/dl vs. 119, 121 ­ 117 ng/dl, p= 0.01) levels, compared to nondepressed women. CONCLUSIONS: These findings suggest that hemostatic factors may be a key me-chanism accounting for the relationship between depression and CHD. [Castilla RC, Bromberger JT, Zhang Y, Perel JM, Matthews KA. Depressive symptoms are related with hemostatic factors in middle-aged women: A report from the Study of Women Health Across the Nation (SWAN). MedUNAB 2004; 7:57-64
Descritores: Mulheres
Fibrinogênio
Hemostáticos
Ativador de Plasminogênio Tecidual
Depressão
Meia-Idade
Limites: Seres Humanos
Responsável: CO179.1 - Biblioteca


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Id: biblio-886414
Autor: Hernández-Ruiz, Eder A; Guarín-Navas, Erika G; Lora-Acuña, Fabián J; Acosta-Reyes, Jorge; Beltrán-Carrascal, Elkin; Meza-Cely, Nohemí.
Título: Trombólisis intravenosa en pacientes con accidente cerebrovascular isquémico: Experiencia de un Hospital del Caribe Colombiano / Intravenous thrombolysis in patients with ischemic stroke: Experience from a Colombian Caribbean Hospital
Fonte: Acta neurol. colomb;33(1):3-7, ene.-mar. 2017. tab, graf.
Idioma: es.
Resumo: RESUMEN INTRODUCCIÓN: Las guías de ACV isquémico establecen dentro del algoritmo de manejo la implementación de la terapia trombolítica intravenosa como una de las estrategias de abordaje del evento agudo que ha demostrado reducir los niveles de discapacidad en los pacientes. OBJETIVO: Describir el impacto de la realización de trombólisis intravenosa en pacientes con accidente cerebro-vascular isquémico, teniendo como referencia escalas de medición de funcionalidad y pronóstico. MATERIALES Y MÉTODOS: Se realizó un estudio descriptivo longitudinal en pacientes que recibieron tratamiento fibrinolítico en la Fundación Hospital Universidad del Norte (octubre 2011 - marzo 2016) teniendo en cuenta aspectos demográficos, clínicos, factores de riesgo y seguimiento a los 30 y 90 días para determinar el pronóstico y la mortalidad según escala modificada de Rankin. RESULTADOS: La población elegible consta de 27 pacientes cuya edad promedio fue de 65 años. La mayor prevalencia se presentó en el género femenino. El factor de riesgo más frecuente fue la hipertensión arterial (19/27). Se realizó manejo inicial de acuerdo al protocolo institucional de la unidad de stroke. Se describen tiempos promedio en cada etapa de la atención médica desde el inicio de los síntomas hasta la realización de la trombolisis. Durante la realización de la intervención terapéutica se documentó las complicaciones que justificaron la suspensión del manejo. Se reportó transformación hemorrágica del ACV y muerte secundaria a ello. La funcionalidad fue valorada con escala NIHSS al ingreso y egreso del paciente, el pronóstico y mortalidad se evaluaron con escala modificada de Rankin, que evidenció que más del 50 % de los pacientes obtuvieron una puntuación entre 0 y 1 a los 90 días postrombólisis. CONCLUSIONES: El estudio presenta limitantes dadas principalmente por el tamaño de la muestra, pero cabe resaltar que refleja la experiencia de un centro universitario y busca establecer y/o generar una base de datos importante a nivel regional que permita ser punto de referencia y suministrar información para futuros estudios.

SUMMARY INTRODUCTION: The ischemic stroke guidelines establish within the management algorithm the implementation of intravenous thrombolytic therapy as one of the strategies to approach the acute event that has been shown to reduce levels of disability in patients. OBJECTIVE: To describe the impact of intravenous thrombolysis performed on patients with ischemic stroke, taking into account functionality and prognosis measuring scales. MATERIALS AND METHODS: We conducted a descriptive longitudinal study on patients to whom intravenous thrombolysis were performed at the Fundación Hospital Universidad del Norte ( October 2011 - March 2016) taking into account the demographics aspects as well as risk factors including a 30 and 90 day follow up to determine prognosis and mortality using the modified Rankin scale. RESULTS: The study consisted of 27 patients whose average age was 65 years, finding a higher prevalence within female patients and A frequent risk factor of hypertension (19/27). Initial management was performed according to the institutional protocol from the stroke unit. Average times were shown at each stage of care, from the onset of symptoms to thrombolysis. During the intervention, some complications were documented which justified the suspension of the treatment. There was hemorrhagic transformation of stroke reported leading to secondary death. Functionality was assessed with the NIHSS scale at patient's admission and discharge, prognosis and mortality were assessed with a modified Rankin scale, which showed that more than 50% of the patients scored between 0 and 1 at 90 days post-thrombolysis. CONCLUSION: The study has limitations in order to establish conclusions statistically significant, but it does reflect the experience of a university center with an effective therapeutic intervention and looks to create a database in the region that can be use like a reference for other studies.
Descritores: Terapia Trombolítica
Ativador de Plasminogênio Tecidual
Colômbia
Acidente Vascular Cerebral
Responsável: CO362.9


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Id: biblio-886413
Autor: Pineda, David A.
Título: Trombólisis con activador recombinante del plasminógeno tisular (rt-PA) para el ataque cerebro vascular agudo: la experiencia colombiana / Thrombolysis with rt-PA for acute stroke: Colombian experience
Fonte: Acta neurol. colomb;33(1):1-2, ene.-mar. 2017.
Idioma: es.
Resumo: RESUMEN El tratamiento con activador recombinante del plasminógeno tisular (sigla en inglés rt-PA), aplicado por vía intravenosa (VIV) es el procedimiento de primera línea en casos de ataque cerebrovascular agudo (ACVA) en una ventana de 4,5 horas. En Colombia hay una experiencia publicada del uso exitoso de este medicamento. En el número actual se publica una nueva experiencia del uso de rt-PA en el caribe colombiano, de gran utilidad para reseñar los artículos publicados en Colombia acerca del tema.

ABSTRACT Treatment with intravenous recombinant tissue plasminogen activator (rt-PA) for acute stroke (AS) is the first line procedure for patients in 4,5 hours window. In Colombia there is a published experience about the successful use of this medication. In the currently issue a new experience from Colombian Caribbean is presented, which it is taken in advantage for briefly reviewing the published papers about the theme in our country.
Descritores: Ativador de Plasminogênio Tecidual
Colômbia
Acidente Vascular Cerebral
Responsável: CO362.9


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Id: biblio-995015
Autor: Castro Hernández, Melissa de; Álvarez Guevara, Angélica Natalia; De los Reyes Valencia, Iliana.
Título: Plasminogenemia asociada a conjuntivitis leñosa / Plasminogen Deficiency and Ligneous Conjunctivitis
Fonte: Univ. med;59(3), 2018. ilus.
Idioma: es.
Resumo: El artículo presenta el caso clínico de un joven de 18 años de edad sin antecedente familiares de enfermedad crónica o hereditaria, nacido a término, sin complicaciones, quien desde los dos días de vida presentó una lesión tipo masa en la conjuntiva tarsal. Inicialmente, recibió tratamiento farmacológico tópico y seguimiento por oftalmología, por conjuntivitis bilateral persistente, masas tarsales recidivantes y cataratas en ambos ojos, por lo que requirió siete intervenciones con pobre respuesta al manejo farmacológico y quirúrgico. A los seis meses de vida se le diagnosticó hidrocefalia, que requirió manejo con derivación del ventrículo peritoneal. Dada la persistencia de la sintomatología y la refractariedad al tratamiento, se ampliaron los estudios y en una junta médica se sugirió el diagnóstico de conjuntivitis leñosa asociada a alteración del plasminógeno. Este diagnóstico fue confirmado por laboratorio clínico, que mostró sus bajas concentraciones de plasminógeno en muestras tomadas con intervalos de dos meses en tres ocasiones: 16,9%, 11,1%, 18,6% (valores de referencia: 70-150%). Se le indicó heparina de bajo peso molecular antes de procedimientos quirúrgicos mayores y triamcinolona tópica según síntomas oculares.

We present a case of an 18-year-old patient without a family history of ocular disease, born full term without complications, within his first 2 days a mass in the tarsal conjunctiva appeared. Initially he received topical treatment and follow-up by the ophthalmology department with a diagnosis of persistent bilateral conjunctivitis, relapsing tarsal masses and cataracts in both eyes requiring a total of 7 surgical interventions with a poor response. At the age of 6 months he was diagnosed with hydrocephalus and required a ventricular-peritoneal shunt. Giren the persistence of the symptoms, further studies were made and a medical board made the diagnosis of ligneous conjunctivitis associated to low levels of plasminogen. The diagnosis was confirmed by decreased levels of plasminogen in serum measured three times with 2 months intervals: 16.9%, 11.1%, 18.6% (reference valúes 70'150%). Low molecular weight heparin was ordered before surgical procedures, and topical triamcinolone applied according to ocular symptoms.
Descritores: Plasminogênio/análise
Fibrina/classificação
Ativador de Plasminogênio Tecidual
-Conjuntivite
Limites: Seres Humanos
Tipo de Publ: Relatos de Casos
Responsável: CO185.1 - Biblioteca Alfonso Borrero Cabal, S. J.


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Id: biblio-937602
Autor: Miranda, Raelson Rodrigues; Soares, Rodolfo Paris.
Título: Avaliação do atendimento a pacientes portadores de acidente vascular cerebral (avc) isquêmico submetidos à trombólise com alteplase.
Fonte: São Paulo; s.n; 2010. 25 p.
Idioma: pt.
Resumo: Série de casos de pacientes que foram admitidos com AVC e realizado tratamento com terapia trombolítica endovenosa. Foram avaliados 14 prontuários de pacientes de fevereiro de 2008 a abril de 2010. Objetivando fazer uma avaliação das características dos pacientes admitidos; avaliar critérios de inclusão e exclusão; avaliar resposta ao tratamento; complicações; tempo de internação; e investigação diagnóstica destes pacientes.
Descritores: Acidente Vascular Cerebral
-Terapia Trombolítica
Ativador de Plasminogênio Tecidual
Limites: Seres Humanos
Responsável: BR2025 - AHM - Autarquia Hospitalar Municipal
BR2025; WL 356, M672a


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Id: biblio-869752
Autor: Achi Arteaga, Jimmy; Arteaga Velez, M. Lilibeth; Zambrano Solorzano, Angel; Peralta, Julia; Martínez Neira, David.
Título: Trombectomía mecánica: técnica de primera elección en el tratamiento ictus isquémico agudo: análisis de beneficios y resultados / Mechanical thrombectomy: first choice therapeutical approach in acute ischemic stroke; analysis of benefits and results
Fonte: Rev. chil. neurocir;42(1):41-44, jul. 2016. ilus, tab.
Idioma: es.
Resumo: Esta revisión tiene como objetivo el uso de la Trombectomía Mecánica como técnica de primera elección en el tratamiento de Ictus Isquémico Agudo. Materiales y Método: La revisión incluye 9 pacientes con diagnóstico de Ictus Isquémico a los que se le realizo Trombectomía Mecánica, se utilizó el dispositivo de recanalización tipo Solitaire asociado a rtPA intraarterial 10 por ciento de la dosis intravenosa, los criterios diagnósticos: edad, período de ventana, imagen de TC al ingreso. Resultados: 8 pacientes (89 por ciento) correspondieron a oclusión de la circulación Anterior, 1 (11 por ciento) a la oclusión de circulación posterior, la efectividad terapéutica se la evalúo según la Escala de Rankin Modificada, 5 (55 por ciento) obtuvieron recuperación completa de sus funciones, 3 (33 por ciento) presentaron secuelas leves, y 1(11 por ciento) no obtuvo mejoría alguna. Discusión: Los resultados de nuestra revisión son alentadores sobre todo al reducir la secuela discapacitante, deja una ventana abierta para posteriores revisiones sobre estetratamiento.

This review aims to use Trombrectomy Mechanics and technique as choice in the treatment of Acute Ischemic Stroke. Materials and Methods: Age, period: The review included 9 patients with a diagnosis of Ischemic Stroke those who underwent Trombrectomy Mechanics, recanalization device type associated with intra-arterial rtPA Solitaire 10 percent of the intravenous dose, the diagnostic criteria was used with CT. Results: 8 patients (89 percent) were occluded circulation Earlier, 1 (11 percent) to occlusion of posterior circulation, the therapeutic succes was evaluated according to the modified Rankin scale, 5 (55 percent) had complete recovery its functions, 3 (33 percent) had mild sequelae, and 1 (11 percent) not obtained any improvement. Discussion: The results of our review are encouraging especially by reducing the disabling sequel, leave a window open for further review on this treatment.
Descritores: Acidente Vascular Cerebral/cirurgia
Isquemia Encefálica
Ativador de Plasminogênio Tecidual
Trombectomia/métodos
-Procedimentos Endovasculares
Procedimentos Neurocirúrgicos/métodos
Limites: Seres Humanos
Tipo de Publ: Estudos de Avaliação
Responsável: CL2.1 - Biblioteca de Medicina


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Id: biblio-860030
Autor: Rodrigues, José Ariévilo Gurgel; Vanderlei, Edfranck de Sousa Oliveira; Quinderé, Ana Luíza Gomes; Fontes, Bruno Pedrosa; Queiroz, Ismael Nilo Lino de; Benevides, Norma Maria Barros.
Título: Glicosaminoglicanos isolados da pele de palombeta (Chloroscombrus chrysurus) e guaiúba (Ocyurus chrysurus): características e implicações biológicas / Glycosaminoglycans isolated from the skin of 'palombeta' (Chloroscombrus chrysurus) and 'guaiúba' (Ocyurus chrysurus): features and biological implications
Fonte: Acta sci., Biol. sci;34(2):141-148, Apr.-June 2012. ilus, tab.
Idioma: en.
Resumo: O Brasil abriga uma das maiores biodiversidades marinhas do mundo, favorecendo a descoberta de fontes alternativas de compostos farmacológicos. Desta forma, objetivou-se avaliar o potencial anticoagulante de glicosaminoglicanos (GAGs) isolados das peles da palombeta (Chloroscombrus chrysurus) e guaiúba (Ocyurus chrysurus). Os GAGs foram extraídos com papaína bruta em tampão acetato de sódio 0,1 M (pH 5,0) contendo cisteína 5 mM e EDTA 5 mM, seguido por cromatografia de troca iônica do extrato total em coluna de DEAE-celulose. As frações obtidas foram analisadas quanto à composição química (proteínas contaminantes e carboidratos totais) e os GAGs identificados por eletroforese em gel de agarose a 0,5%. Os ensaios de atividade anticoagulante foram realizados por meio do tempo de tromboplastina parcial ativada (TTPA) usando plasma humano normal e heparina-padrão (193,00 UI mg-1). O procedimento de obtenção e fracionamento dos GAGs mostrou-se eficiente, indicando semelhantes perfis cromatográficos entre as espécies avaliadas e, revelando para C. chrysurus, bandas com mobilidades semelhantes ao dermatam sulfato e com atividade de apenas 3,30 UI mg-1.

A great number of pharmacological compounds is found in the Brazilian marine diversity. This study evaluated the anticoagulant potential of glycosaminoglycans (GAGs) isolated from the skin of 'palombeta' Chloroscombrus chrysurus and 'guaiúba' Ocyurus chrysurus. GAGs were extracted with crude papain in 0.1 M sodium acetate buffer (pH 5.0) containing 5 mM cysteine and 5 mM EDTA, followed by ion exchange chromatography on DEAE-cellulose column. The chemical composition (contaminant proteins and total carbohydrates) and the analysis by 0.5% agarose gel electrophoresis of fractions were also determined. Anticoagulant assays were performed by activated partial thromboplastin time (APTT) using normal human plasma and standard heparin (193.00 IU mg-1). The obtaining and fractionation procedures of GAGs were effective and similar chromatographic profiles were verified between the species. A similar mobility to dermatan sulfate was revealed for C. chrysurus. This GAG also showed a low activity of 3.30 IU mg-1.
Descritores: Biodiversidade
Coagulação Sanguínea
Glicosaminoglicanos
Ambiente Marinho
Farmacologia
Ativador de Plasminogênio Tecidual
Responsável: BR513.2 - Editora da Universidade Estadual de Maringá



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