Base de dados : LILACS
Pesquisa : D08.811.464.259.850 [Categoria DeCS]
Referências encontradas : 8 [refinar]
Mostrando: 1 .. 8   no formato [Detalhado]

página 1 de 1

  1 / 8 LILACS  
              next record last record
seleciona
para imprimir
Fotocópia
Texto completo
Id: biblio-1283600
Autor: Oestreich, Arne Michael; Ilire Suli, Merlinda; Gerlach, Doreen; Fan, Rong; Czermak, Peter.
Título: Media development and process parameter optimization using statistical experimental designs for the production of nonribosomal peptides in Escherichia coli
Fonte: Electron. j. biotechnol;52:85-92, July. 2021. graf, tab.
Idioma: en.
Resumo: BACKGROUND: Nonribosomal peptide synthases (NRPS) can synthesize functionally diverse bioactive peptides by incorporating nonproteinogenic amino acids, offering a rich source of new drug leads. The bacterium Escherichia coli is a well-characterized production host and a promising candidate for the synthesis of nonribosomal peptides, but only limited bioprocess engineering has been reported for such molecules. We therefore developed a medium and optimized process parameters using the design of experiments (DoE) approach. RESULTS: We found that glycerol is not suitable as a carbon source for rhabdopeptide production, at least for the NRPS used for this study. Alternative carbon sources from the tricarboxylic acid cycle achieved much higher yields. DoE was used to optimize the pH and temperature in a stirred-tank reactor, revealing that optimal growth and optimal production required substantially different conditions. CONCLUSIONS: We developed a chemically defined adapted M9 medium matching the performance of complex medium (lysogeny broth) in terms of product concentration. The maximum yield in the reactor under optimized conditions was 126 mg L-1, representing a 31-fold increase compared to the first shaking-flask experiments with M9 medium and glycerol as the carbon source. Conditions that promoted cell growth tended to inhibit NRPS productivity. The challenge was therefore to find a compromise between these factors as the basis for further process development.
Descritores: Peptídeo Sintases/metabolismo
Reatores Biológicos/microbiologia
Escherichia coli
-Temperatura
Biotecnologia
Carbono/metabolismo
Modelos Estatísticos
Eletroforese em Gel de Poliacrilamida
Bioengenharia
Concentração de Íons de Hidrogênio
Responsável: CL1.1 - Biblioteca Central


  2 / 8 LILACS  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo SciELO Chile
Texto completo
Id: biblio-950907
Autor: Meng, Minjun; Chen, Yanling; Jia, Jianbo; Li, Lianghui; Yang, Sumei.
Título: Knockdown of PAICS inhibits malignant proliferation of human breast cancer cell lines
Fonte: Biol. Res;51:24, 2018. tab, graf.
Idioma: en.
Resumo: BACKGROUND: Phosphoribosylaminoimidazole carboxylase, phosphoribosylaminoimidazole succinocarboxamide synthetase (PAICS), an enzyme required for de novo purine biosynthesis, is associated with and involved in tumorigenesis. This study aimed to evaluate the role of PAICS in human breast cancer, which remains the most frequently diagnosed cancer and the leading cause of cancer-related death among women in less developed countries. RESULTS: Lentivirus-based short hairpin RNA targeting PAICS specifically depleted its endogenous expression in ZR-75-30 and MDA-MB-231 breast cancer cells. Depletion of PAICS led to a significant decrease in cell viability and proliferation. To ascertain the mechanisms through which PAICS modulates cell proliferation, flow cytometry was performed, and it was confirmed that G1-S transition was blocked in ZR-75-30 cells through PAICS knockdown. This might have occurred partly through the suppression of Cyclin E and the upregulation of Cyclin D1, P21, and CDK4. Moreover, PAICS knockdown obviously promoted cell apoptosis in ZR-75-30 cells through the activation of PARP and caspase 3 and downregulation of Bcl-2 and Bcl-xl expression in ZR-75-30 cells. CONCLUSIONS: These findings demonstrate that PAICS plays an essential role in breast cancer proliferation in vitro, which provides a new opportunity for discovering and identifying novel effective treatment strategies.
Descritores: Peptídeo Sintases/fisiologia
Neoplasias da Mama/enzimologia
Neoplasias da Mama/patologia
Carboxiliases/biossíntese
Biomarcadores Tumorais/fisiologia
Proliferação de Células
-Peptídeo Sintases/genética
Regulação Enzimológica da Expressão Gênica
Regulação Neoplásica da Expressão Gênica
Linhagem Celular Tumoral
Técnicas de Silenciamento de Genes
Citometria de Fluxo
Limites: Humanos
Feminino
Responsável: CL1.1 - Biblioteca Central


  3 / 8 LILACS  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo SciELO Brasil
Texto completo
Id: biblio-1130108
Autor: Tomaseto, Alex Augusto; Alpiste, Marcel Costa; Nassar, Alessandra Figueiredo de Castro; Destéfano, Suzete Aparecida Lanza.
Título: Antibacterial activity of phytopathogenic Streptomyces strains against bacteria associated to clinical diseases / Atividade antimicrobiana de Streptomyces fitopatogênicas contra bactérias associadas a doenças de importância clínica
Fonte: Arq. Inst. Biol;87:e0142020, 2020. tab.
Idioma: en.
Projeto: Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - Brasil; . Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq).
Resumo: The genus Streptomyces is associated with the ability to produce and excrete a variety of bioactive compounds, such as antibiotic, antifungal and antiviral. Biological active polyketide and peptide compounds with applications in medicine, agriculture and biochemical research are synthesized by PKS-I and NRPS genes. The evaluation of the presence of these genes associated with the biosynthesis of secondary metabolites in different phytopathogenic Streptomyces strains were performed using degenerated primers. The positive signal was observed in 58/63 Streptomyces strains for NRPS gene, 43/63 for PKS-I, and for PKS-II all the 63 strains showed positive signal of amplification. These strains also were tested with double layer agar-well technique against bacterial with clinical importance, and it was possible to observe the Streptomyces spp. strains were able to inhibit the growth of 14, 20, 13 and 3 isolates Gram-positive and Gram-negative bacteria, Staphylococcus aureus (ATCC 25923), Bacillus cereus (ATCC 14579), Pseudomonas aeruginosa (ATCC 27853) and Escherichia coli (ATCC 11775) respectively. The Streptomyces sp. strains IBSBF 2019 and IBSBF 2397 showed antibacterial activity against all four bacteria-target tested.(AU)

O gênero Streptomyces apresenta alta capacidade de produzir e excretar uma grande variedade de compostos biologicamente ativos, como antibióticos, antifúngicos e antivirais. Compostos biologicamente ativos de policetídeos e peptídeos com aplicações na medicina, agricultura e pesquisas bioquímicas são sintetizados pelos genes PKS-I e NRPS. A avaliação da presença desses genes associados à biossíntese de metabólitos secundários em diferentes linhagens de Streptomyces fitopatogênicas foi realizada através do uso de primers degenerados. O sinal positivo foi observado em 58/63 linhagens de Streptomyces para o gene NRPS, 43/63 para o gene PKS-I e, para o gene PKS-II, todas as 63 linhagens apesentaram o sinal positivo de amplificação. Essas linhagens também foram testadas através da técnica de dupla camada contra bactérias de importância clínica e foi possível observar que as linhagens de Streptomyces spp. foram capazes de inibir o crescimento de 14, 20, 13 e 3 isolados de bactérias Gram-positivas e Gram-negativas, Staphylococcus aureus (ATCC 25923), Bacillus cereus (ATCC 14579), Pseudomonas aeruginosa (ATCC 27853) e Escherichia coli (ATCC 11775), respectivamente. As linhagens de Streptomyces sp. ISBSF 2019 e 2397 apresentaram atividade antibacteriana contra todas as bactérias-alvo testadas.(AU)
Descritores: Pseudomonas aeruginosa/crescimento & desenvolvimento
Staphylococcus aureus/crescimento & desenvolvimento
Streptomyces/metabolismo
Bacillus cereus/crescimento & desenvolvimento
Escherichia coli/crescimento & desenvolvimento
Antibacterianos/metabolismo
-Peptídeo Sintases/genética
Streptomyces/genética
Amplificação de Genes
Reação em Cadeia da Polimerase
Análise de Sequência de DNA
Primers do DNA
Policetídeo Sintases/genética
Antibacterianos/farmacologia
Responsável: BR1942.1 - NID - Biblioteca - Núcleo de Informação e Documentação


  4 / 8 LILACS  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo SciELO Brasil
Texto completo
Id: biblio-894834
Autor: Souza, Claudemir; Zanchin, Nilson IT; Krieger, Marco A; Ludwig, Adriana.
Título: In silico analysis of amino acid variation in human respiratory syncytial virus: insights into immunodiagnostics
Fonte: Mem. Inst. Oswaldo Cruz;112(10):655-663, Oct. 2017. tab, graf.
Idioma: en.
Projeto: FINEP; . BNDES.
Resumo: BACKGROUND The highly contagious nature of human respiratory syncytial virus (HRSV) and the gravity of its infection in newborns and vulnerable adults pose a serious public health problem. Thus, a rapid and sensitive diagnostic test for viral detection that can be implemented upon the first appearance of symptoms is needed. The genetic variation of the virus must be considered for immunodiagnostic purposes. OBJECTIVES To analyse HRSV genetic variation and discuss the possible consequences for capture immunoassay development. METHODS We performed a wide analysis of N, F and G protein variation based on the HRSV sequences currently available in the GenBank database. We also evaluated their similarity with homologous proteins from other viruses. FINDINGS The mean amino acid divergences for the N, F, and G proteins between HRSV-A and HRSV-B were determined to be approximately 4%, 10% and 47%, respectively. Due to their high conservation, assays based on the full-length N and F proteins may not distinguish HRSV from human metapneumovirus and other Mononegavirales viruses, and the full-length G protein would most likely produce false negative results due to its high divergence. MAIN CONCLUSIONS We have identified specific regions in each of these three proteins that have higher potential to produce specific results, and their combined utilisation should be considered for immunoassay development.
Descritores: Peptídeo Sintases
Vírus Sinciciais Respiratórios
Variação Genética
Proteínas Virais/genética
Genótipo
-Filogenia
Testes Imunológicos
Limites: Humanos
Responsável: BR1.1 - BIREME


  5 / 8 LILACS  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Id: lil-503745
Autor: Ferrer, Elizabeth.
Título: Teniasis/cisticercosis: avances en diagnóstico inmunológico y molecular / Taeniasis/cysticercosis: advances in inmunological and molecular diagnosis
Fonte: Bol. malariol. salud ambient;46(1):1-13, 2006.
Idioma: es.
Resumo: La teniasis es la infección parasitaria producida por el adulto de Taenia solium y T. saginata, mientras que la cisticercosis es causada por el estadío larvario (cisticerco) de estos ténidos enhospedadores intermediarios; el hombre puede de forma accidental adquirir la cisticercosis. El binomio teniasis/cisticercosis causa graves problemas de salud pública y económicos en las zonas endémicas de África, Asia, y Latinoamérica, además de otras áreas como consecuencia de los viajes y las migraciónes. La neurocisticercosis es la enfermedad parasitaria más importante del sistema nervioso central. El diagnóstico de la teniasis se logra generalmente mediante examenes coprológicos, mientras que el diagnóstico de la cisticercosis se lleva a cabo por métodos parasitológicos, por técnicas de imágenes y una amplia variedad de ensayos inmunológicos. Los métodos de diagnóstico inmunológico convencional presentan graves limitaciones, baja sensibilidad y especificidad, no estandarizados convenientemente y basados en la utilización como antígeno del siempre escaso material parasitario. Actualmente se están utilizando nuevas herramientas y técnicas que permiten un mejor diagnóstico de estas enfermedades, por ejemplo, anticuerpos monoclonales, antígenos recombinantes, péptidos sintéticos, PCR, cuya manipulación es de fácil estandarización e independientes de las fuentes del siempre preciado material parasitario.
Descritores: Cisticercose/diagnóstico
Doenças Parasitárias
Peptídeo Sintases
Teníase/diagnóstico
-Parasitologia
Saúde Pública
Venezuela
Limites: Humanos
Masculino
Feminino
Tipo de Publ: Revisão
Responsável: VE1.1 - Biblioteca Humberto Garcia Arocha


  6 / 8 LILACS  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
Id: lil-441046
Autor: Roth, D. M; Senna, J. P. M; Machado, D. C.
Título: Evaluation of the humoral immune response in BALB/c mice immunized with a naked DNA vaccine anti-methicillin-resistant Staphylococcus aureus
Fonte: Genet. mol. res. (Online);5(3):503-512, 2006. ilus, tab, graf.
Idioma: en.
Resumo: Methicillin-resistant Staphylococcus aureus (MRSA) is the major pathogen involved in nosocomial infections, leading to high rates of morbidity and mortality in hospitals worldwide. The methicillin resistance occurs due to the presence of an additional penicillin-binding protein, PBP2a, which has low affinity for b-lactam antibiotics. In the past few years, vancomycin has been the only antibiotic option for treatment of infections caused by multiresistant MRSA; however, reports of vancomycin-resistant strains have generated great concerns regarding the treatment to overcome these infections. In the present study, we report preliminary results regarding the humoral immune response generated in BALB/c mice by two different doses of naked DNA vaccine containing an internal region, comprising the serine-protease domain, of the PBP2a of MRSA. The immunization procedure consisted of four immunizations given intramuscularly within 15-day intervals. Blood was collect weekly and anti-PBP2a-specific antibodies were screened by ELISA. BALB/c mice immunized with DNA vaccine anti-PBP2a have shown higher antibody titers mainly after the fourth immunization, and intriguingly, no correlation between the humoral immune response and DNA dose was observed. Our results suggest that the DNA vaccine anti-PBP2a induced an immune response by production of specific antibodies anti-MRSA in a non-dose-dependent manner, and it could represent a new and valuable approach to produce specific antibodies for passive immunization to overcome MRSA infections.
Descritores: Anticorpos Antibacterianos/biossíntese
Resistência a Meticilina/efeitos dos fármacos
Proteínas de Ligação às Penicilinas/imunologia
Peptídeo Sintases/imunologia
Vacinas Antiestafilocócicas/administração & dosagem
Staphylococcus aureus/imunologia
Vacinas de DNA/administração & dosagem
-Eletroforese em Gel de Poliacrilamida
Ensaio de Imunoadsorção Enzimática
Camundongos Endogâmicos BALB C
Resistência a Meticilina/imunologia
Reação em Cadeia da Polimerase
Vacinas Antiestafilocócicas/imunologia
Vacinas de DNA/imunologia
Limites: Humanos
Animais
Camundongos
Responsável: BR1.1 - BIREME


  7 / 8 LILACS  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Id: lil-344367
Autor: Arce C., Mauricio; Mercado G., Javier; Hinojosa C., Walter.
Título: Oxido nitrico de ilustre desconocido a motor fisiologico / Nitric oxide: a review
Fonte: Cuad. Hosp. Clín = Cuad. - Hosp. clín;48(1):87-96, 2003. ilus.
Idioma: es.
Resumo: En las últimad décadas el óxido nítrico ha pasado de ser un contaminante ambiental a una mólecula implicada en múltiples funciones fisiológicas, actualmente se han multiplicado las investigaciones en torno a sus funciones. El óxido nítrico juega un papel imortnate en la regulación de una serie de procesos fisiológicos como ser: el tono vasomotor, la motilidad intestinal, actúa como un neurotransmisor central y periférico, desemeña un papel fundamental en la funciones del sistema inmune y la fisiologia del os procesos de agregación plaquetaria y leucocitaria. Sus acciones fisioatológiocas incluyen un rol determinante en la génesis del Shock séptico, en el daño tisular resultado de la inflamación, en el envejecimiento motocondrial, en el desarrollo dela rtrosis y la artritis reumatoidea, enfermedades degenerativas dels istema nervioso central y en el daño producido por la isquemia miocárdica y cerebral. Demostrando múltiples aplicaciones terapéuticas en el tratamiento de varias patologías.
Descritores: Poluição Ambiental/efeitos adversos
Poluição Ambiental/estatística & dados numéricos
Poluição Ambiental/prevenção & controle
Óxido Nítrico/efeitos adversos
Peptídeo Sintases
Substâncias Tóxicas
Responsável: BO6.1 - Biblioteca


  8 / 8 LILACS  
              first record previous record
seleciona
para imprimir
Fotocópia
Texto completo SciELO Brasil
Texto completo
Id: lil-218657
Autor: Noya, Oscar; Noya, Belkisyolé Alarcón de; Ballén, Diana; Zerpa, Noraida; Colmenares, Cecília; Losada, Sandra; Bermudez, Henry.
Título: Use of synthetic peptides derived from adult worm proteins of Schistosoma mansoni, in the diagnosis of schistosomiasis
Fonte: Mem. Inst. Oswaldo Cruz;93(supl.1):157-8, Oct. 1998. tab.
Idioma: en.
Conferência: Apresentado em: Simpósio Internacional sobre Esquistossomose, 6 e Reuniäo Nacional sobre Esquistossomose, 6, Belo Horizonte, Oct. 19-24, 1997.
Descritores: Peptídeo Sintases
Schistosoma mansoni/enzimologia
Esquistossomose mansoni/diagnóstico
Limites: Animais
Responsável: BR15.1 - Biblioteca de Ciências Biomédicas



página 1 de 1
   


Refinar a pesquisa
  Base de dados : Formulário avançado   

    Pesquisar no campo  
1  
2
3
 
           



Search engine: iAH v2.6 powered by WWWISIS

BIREME/OPAS/OMS - Centro Latino-Americano e do Caribe de Informação em Ciências da Saúde