Base de dados : LILACS
Pesquisa : D08.811.464.938.750.210.750 [Categoria DeCS]
Referências encontradas : 3 [refinar]
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Id: biblio-886260
Autor: Wang, Wei; Wang, Qi; Yu, Wanyou; Chen, Lianhua; Li, Zhong.
Título: Efficacy of phosphocreatine pre-administration on XIAP and Smac in ischemic penumbra of rats with focal cerebral ischemia reperfusion injury
Fonte: Acta cir. bras;33(2):117-124, Feb. 2018. tab, graf.
Idioma: en.
Resumo: Abstract Purpose: To observe the efficacy of phosphocreatine pre-administration (PCr-PA) on X-linked inhibitor of apoptosis protein (XIAP), the second mitochondia-derived activator of caspase (Smac) and apoptosis in the ischemic penumbra of rats with focal cerebral ischemia-reperfusion injury (CIRI). Methods: A total of 60 healthy male Sprague Dawley (SD) rats were randomly divided into three groups (n=20): group A (the sham operation group), group B <intraperitoneally injected with 20 mg/kg (10 mg/ml) of saline before preparing the ischemia-reperfusion (IR) model>, and group C <intraperitoneally injected with 20 mg/kg (10 mg/ml) of PCr immediately before preparing the IR model>. After 24 h for reperfusion, the neurological function was evaluated and the tissue was sampled to detect expression of XIAP, Smac and caspase-3 positive cells in the ischemic penumbra so as to observe the apoptosis. Results: Compared with group B, neurological deficit scores, numbers of apoptotic cells, expression of Smac,caspase-9 and the numbers of Caspase-3 positive cells were decreased while expression of XIAP were increased in the ischemic penumbra of group C. Conclusions: Phosphocreatine pre-administration may elicit neuroprotective effects in the brain by increasing expression of X-linked inhibitor of apoptosis protein, reducing expression of second mitochondia-derived activator of caspase, and inhibiting the apoptosis in the ischemic penumbra.
Descritores: Fosfocreatina/farmacologia
Cardiotônicos/farmacologia
Traumatismo por Reperfusão/metabolismo
Isquemia Encefálica/metabolismo
Proteínas Mitocondriais/metabolismo
Peptídeos e Proteínas de Sinalização Intracelular/metabolismo
Proteínas Inibidoras de Apoptose Ligadas ao Cromossomo X/metabolismo
-Distribuição Aleatória
Isquemia Encefálica/prevenção & controle
Ratos Sprague-Dawley
Apoptose/efeitos dos fármacos
Fármacos Neuroprotetores/farmacologia
Modelos Animais de Doenças
Avaliação Pré-Clínica de Medicamentos
Proteínas Reguladoras de Apoptose
Caspase 3/metabolismo
Limites: Humanos
Animais
Masculino
Ratos
Responsável: BR1.1 - BIREME


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Colli, Benedicto Oscar
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Id: lil-795996
Autor: Carvalho, Camila Albuquerque Melo de; Tirapelli, Daniela Pretti da Cunha; Rodrigues, Andressa Romualdo; Lizarte Neto, Fermino Sanches; Novais, Paulo Cézar; Silva, Jairo Pinheiro; Carlotti Júnior, Carlos Gilberto; Colli, Benedicto Oscar; Tirapelli, Luís Fernando.
Título: Morphological and immunohistochemical analysis of apoptosis in the cerebellum of rats subjected to focal cerebral ischemia with or without alcoholism model
Fonte: Acta cir. bras;31(9):629-637, Sept. 2016. graf.
Idioma: en.
Resumo: ABSTRACT PURPOSE: To evaluated histopathological changes, morphometric and expression of proteins CASPASE-3, BCL-2 and XIAP related to apoptosis in the cerebellum after induction of temporary focal cerebral ischemia followed by reperfusion, with or without a model of chronic alcoholism. METHODS: Fifty Wistar rats were used and divided into: control group (C), sham group (S), ischemic group (I), alcoholic group (A), and ischemic and alcoholic group (IA). The cerebellum samples collected were stained for histopathological and morphometric analysis and immunohistochemistry study. RESULTS: Histopathological changes were observed a greater degree in animals in groups A and IA. The morphometric study showed no difference in the amount of cells in the granular layer of the cerebellum between the groups. The expression of CASPASE-3 was higher than BCL-2 and XIAP in the groups A and IA. CONCLUSION: We observed correlation between histopathological changes and the occurrence of apoptosis in cerebellar cortex.
Descritores: Cerebelo/patologia
Isquemia Encefálica/patologia
Apoptose
Etanol/farmacologia
Alcoolismo/patologia
Proteínas Reguladoras de Apoptose/metabolismo
-Imuno-Histoquímica
Traumatismo por Reperfusão/patologia
Cerebelo/efeitos dos fármacos
Cerebelo/metabolismo
Isquemia Encefálica/metabolismo
Ratos Wistar
Estatísticas não Paramétricas
Proteínas Proto-Oncogênicas c-bcl-2/metabolismo
Modelos Animais de Doenças
Alcoolismo/metabolismo
Proteínas Inibidoras de Apoptose Ligadas ao Cromossomo X/metabolismo
Caspase 3/metabolismo
Limites: Animais
Masculino
Responsável: BR1.1 - BIREME


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Id: lil-614545
Autor: Wang, Juan-Juan; Li, Yu-Feng; Jin, Ying-Ying; Wang, Xi; Chen, Tong-Xin.
Título: Effects of Epstein-Barr virus on the development of dendritic cells derived from cord blood monocytes: an essential role for apoptosis
Fonte: Braz. j. infect. dis;16(1):19-26, Jan.-Feb. 2012. ilus.
Idioma: en.
Projeto: National Natural Science Foundation of China; . Science and Technology Commission of Shanghai Municipality; . PhD Programs Foundation of Ministry of Education of China; . Shanghai Municipal Education Commission; . Shanghai Municipal Health Bureau; . Pudong New District Social Development Bureau; . PhD Programs Foundation of Shanghai Jiao Tong University School of Medicine.
Resumo: OBJECTIVE: Epstein-Barr virus (EBV) is a ubiquitous human γ-herpes virus, which can adapt and evade host immune defense. Dendritic cells (DCs) play a pivotal role in the initiation and maintenance of immune responses. This study investigated the effects of EBV on cord blood monocytes derived DCs (CBDC). METHODS: Monocytes were isolated from cord blood and cultured in medium containing recombinant IL-4 and GM-CSF to induce DCs development. B95-8 supernatant was added in monocytes culture medium for EBV infection at day 0. Phenotypic characterization of DCs, apoptotic cells, and mitochondrial membrane potential (MMP) were detected by flow cytometry. The morphology was observed by Hoechst 33258 staining and TUNEL staining, the expression of X-linked inhibitor of apoptosis protein (XIAP) was detected by Western blotting assay and caspase 3, 8 and 9 activity was measured. RESULTS: Phenotypic characterization of DCs was changed in EBV-treated group. Chromatin condensation and DNA fragmentation were observed in EBV induced CBDC apoptosis. In addition, caspase 3, caspase 8, and caspase 9 activation were enhanced in the EBV-treated group. This was accompanied by the loss of MMP. Furthermore, XIAP expression was down-regulated in the EBV-treated group and compared to mock-infected group. CONCLUSION: These results suggested that EBV could inhibit CBDC phenotypic differentiation, and induce CBDC apoptosis in caspase-dependent manner with involvement of the mitochondrial pathway. This might help EBV to evade host immune responses to establish persistent infection.
Descritores: Apoptose/fisiologia
Efeito Citopatogênico Viral/fisiologia
Células Dendríticas/patologia
Sangue Fetal/citologia
/fisiologia
HERPESVIRUS ABBREVIATIONS AS TOPIC, HUMAN/fisiologia
Monócitos/patologia
-Western Blotting
Diferenciação Celular
Caspases/imunologia
Células Dendríticas/virologia
Citometria de Fluxo
/imunologia
HERPESVIRUS ABBREVIATIONS AS TOPIC, HUMAN/imunologia
/imunologia
INTERLEUKIN-ABBREVIATIONS AS TOPIC/imunologia
Monócitos/citologia
Monócitos/virologia
Fenótipo
Proteínas Inibidoras de Apoptose Ligadas ao Cromossomo X/imunologia
Limites: Humanos
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME



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