Base de dados : LILACS
Pesquisa : D08.811.520.224.125.800 [Categoria DeCS]
Referências encontradas : 4 [refinar]
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Id: biblio-1116300
Autor: Contreras, Roberto; van den Brink, Liesbeth; Sepúlveda, Bernardo; Aguayo, Fernanda; Porcile, Vincenzo.
Título: Phylogenetic relationships of plant species from the flowering desert of the Atacama Region / Relaciones filogenéticas de especies de plantas que crecen en el desierto florido de la Región de Atacama
Fonte: Bol. latinoam. Caribe plantas med. aromát;19(3):300-313, mayo 2020. ilus, tab.
Idioma: en.
Projeto: Regional Innovation Fund for Regional Competitiveness; . Regional Government of Atacama; . National Forestry Corporation.
Resumo: Every 3 to 7 year angiosperms species of the flowering desert appear in the Atacama Region of Chile, as a result of the climatic phenomenon "El Niño". Our objective was to evaluate the universality of matK and rbcL barcode markers of these species, and validate their taxon through phylogenetic relationships. Argemone hunnemannii, Oenothera coquimbensis, Malesherbia humilis, Leucocoryne appendiculata, Loasa elongata, Nicotiana solanifolia, Stachys grandidentata, Aristolochia chilensis, Alstroemeria kingii and Adesmia eremophila, almost all classified as endemic to Chile, were collected in Pan de Azúcar and Llanos de Challe National Park (Atacama Region, Chile) at the end of October 2017. The phylogeny of these ten angiosperm species from the flowering desert was analyzed using rbcL and matK markers with the maximum likelihood and Bayesian inference methods. The results showed that 70% of the species can be distinguished with the matK or rbcL locus, however, 100% were distinguished using both loci. The phylogenetic results showed that the species formed clades with high reliability and high support with both the matK and rbcL genes, when comparing our results with sequences obtained from GenBank. The matK and rbcL genes are efficient markers for analyzing phylogenetic relationships and validating the taxonomy of flowering species.

Las especies de angiospermas del Desierto Florido de la Región de Atacama de Chile aparecen cada 3 a 7 años, influenciado por el fenómeno climático "El Niño". Nuestro objetivo fue evaluar la universalidad de los marcadores de código de barra matK y rbcL de estas especies, y validar su taxón por medio de relaciones filogenéticas. Las especies Argemone hunnemannii, Oenothera coquimbensis, Malesherbia humilis, Leucocoryne appendiculata, Loasa elongata, Nicotiana solanifolia, Stachys grandidentata, Aristolochia chilensis, Alstroemeria kingii y Adesmia eremophila son clasificadas la mayoría endémicas de Chile. Estas especies fueron colectadas en el Parque Nacional Pan de Azúcar y Llanos de Challe, Región de Atacama, Chile. La colecta se realizó a fines de octubre de 2017. Con los marcadores rbcL y matK se analizó la filogenia con los métodos máxima verosimilitud e inferencia bayesiana en diez especies de angiosperma del Desierto Florido. Los resultados mostraron que el 70% de las especies pueden ser distinguidas con un locus matK o rbcL, sin embargo, el 100% se distinguió usando ambos locus. Los resultados filogenéticos mostraron que las especies formaron clados con alta fiabilidad y alto soporte tanto con los genes matK y rbcL, al comparar con accesos de secuencias obtenidas de GenBank. Lo genes matK y rbcL son marcadores eficientes para analizar relaciones filogenéticas y validar el taxón de las especies de flor.
Descritores: Filogenia
Plantas/genética
Deserto
Código de Barras de DNA Taxonômico/métodos
-Ribulose-Bifosfato Carboxilase
Chile
Análise de Sequência de DNA
Responsável: CL1.1 - Biblioteca Central


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Texto completo SciELO Brasil
Possas, Cristina de Albuquerque
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Id: lil-741621
Autor: Martins, Reinaldo de Menezes; Possas, Cristina de Albuquerque; Homma, Akira.
Título: Historical review of clinical vaccine studies at Oswaldo Cruz Institute and Oswaldo Cruz Foundation - technological development issues
Fonte: Mem. Inst. Oswaldo Cruz;110(1):114-124, 03/02/2015. tab.
Idioma: en.
Resumo: This paper presents, from the perspective of technological development and production, the results of an investigation examining 61 clinical studies with vaccines conducted in Brazil between 1938-2013, with the participation of the Oswaldo Cruz Institute (IOC) and the Oswaldo Cruz Foundation (Fiocruz). These studies have been identified and reviewed according to criteria, such as the kind of vaccine (viral, bacterial, parasitic), their rationale, design and methodological strategies. The results indicate that IOC and Fiocruz have accumulated along this time significant knowledge and experience for the performance of studies in all clinical phases and are prepared for the development of new vaccines products and processes. We recommend national policy strategies to overcome existing regulatory and financing constraints.
Descritores: Ração Animal/efeitos adversos
Proteínas na Dieta/química
Modelos Biológicos
Proantocianidinas/química
Rúmen/metabolismo
-Brassica rapa/química
Precipitação Química
Proteínas na Dieta/metabolismo
Fermentação
Fabaceae/efeitos adversos
Fabaceae/química
Frutas/efeitos adversos
Frutas/química
Estrutura Molecular
Peso Molecular
Concentração Osmolar
Proteínas de Plantas/química
Proteínas de Plantas/metabolismo
Proantocianidinas/efeitos adversos
Proantocianidinas/metabolismo
Ruminantes
Ribulose-Bifosfato Carboxilase/química
Ribulose-Bifosfato Carboxilase/metabolismo
Rúmen/microbiologia
Solubilidade
Estereoisomerismo
Soroalbumina Bovina/química
Soroalbumina Bovina/metabolismo
Limites: Animais
Tipo de Publ: Estudo Comparativo
Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


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Texto completo SciELO Chile
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Id: lil-468185
Autor: Leitao, Louis; Maoret, Jean-José; Biolley, Jean-Philippe.
Título: Changes in PEP Carboxylase, Rubisco and Rubisco activase mRNA levels from maize (Zea mays) exposed to a chronic ozone stress
Fonte: Biol. Res;40(2):137-153, 2007. graf, tab.
Idioma: en.
Resumo: We quantified the ozone impact on levels of Zea mays L. cv. Chambord mRNAs encoding C4-phosphoenolpyruvate carboxylase (C4-PEPc), ribulose-l,5-bisphosphate carboxylase/oxygenase small and large subunits (Rubisco-SSU and Rubisco-LSU, respectively) and Rubisco activase (RCA) using real-time RT-PCR. Foliar pigment content, PEPc and Rubisco protein amounts were simultaneously determined. Two experiments were performed to study the ozone response of the 5th and the 10th leaf. For each experiment, three ozone concentrations were tested in open-top chambers: non-filtered air (NF, control) and non-filtered air containing 40 (+40) and 80 nL L-1 (+80) ozone. Regarding the 5th leaf, +40 atmosphere induced a loss in pigmentation, PEPc and Rubisco activase mRNAs. However, it was unable to notably depress carboxylase protein amounts and mRNAs encoding Rubisco. Except for Rubisco mRNAs, all other measured parameters from 5th leaf were depressed by +80 atmosphere. Regarding the 10th leaf, +40 atmosphere increased photosynthetic pigments and transcripts encoding Rubisco and Rubisco activase. Rubisco and PEPc protein amounts were not drastically changed, even if they tended to be increased. Level of C4-PEPc mRNA remained almost stable. In response to +80 atmosphere, pigments and transcripts encoding PEPc were notably decreased. Rubisco and PEPc protein amounts also declined to a lesser extent. Conversely, the level of transcripts encoding both Rubisco subunits and Rubisco activase that were not consistently disturbed tended to be slightly augmented. So, the present study suggests that maize leaves can respond differentially to a similar ozone stress.
Descritores: Ozônio/farmacologia
Fosfoenolpiruvato Carboxilase/metabolismo
Ribulose-Bifosfato Carboxilase/metabolismo
Zea mays/efeitos dos fármacos
Zea mays/enzimologia
-Fosfoenolpiruvato Carboxilase/efeitos dos fármacos
Folhas de Planta/efeitos dos fármacos
Folhas de Planta/enzimologia
Proteínas de Plantas/metabolismo
Reação em Cadeia da Polimerase Via Transcriptase Reversa
RNA Mensageiro/efeitos dos fármacos
RNA de Plantas/efeitos dos fármacos
Ribulose-Bifosfato Carboxilase/efeitos dos fármacos
Zea mays/genética
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


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Fotocópia
Id: lil-310754
Autor: Gómez García, Felipe.
Título: Eficacia del tratamiento farmacológico de osteoporosis en la prevención de fracturas / Pharmacological treatment efficacy in osteoporosis for prevention of fractures
Fonte: Rev. mex. ortop. traumatol;15(2):78-93, mar.-abr. 2001. tab.
Idioma: es.
Resumo: En el presente artículo se hace una exhaustiva revisión bibliográfica de las investigaciones y resultados clínicos de las drogas más utilizadas para el tratamiento de la osteoporosis, así como de la efectividad de cada una de ellas para prevenir una fractura. También se discuten los problemas más importantes para prevenir, diagnosticar y tratar esta enfermedad con un costo beneficio razonable. Al final se discuten algunas estrategias y sugerencias para definir si es necesaria o no una intervención farmacológica, y si ésta se considera necesaria, de qué tipo debe ser.
Descritores: Alendronato
Calcitonina
Difosfonatos
Fluoretos
Fraturas Ósseas/prevenção & controle
Osteoporose Pós-Menopausa/tratamento farmacológico
Ribulose-Bifosfato Carboxilase
-Análise Custo-Benefício/estatística & dados numéricos
Resultado do Tratamento
Tipo de Publ: Revisão
Responsável: MX1.1 - CENIDSP - Centro de Información para Decisiones en Salud Pública



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