Base de dados : LILACS
Pesquisa : D08.811.520.232.400.700 [Categoria DeCS]
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Id: biblio-1010394
Autor: Xie, Jian-Hui; Chai, Ting-Ting; Xu, Ran; Liu, Dan; Yang, Yu-Xiu; Deng, Zhi-Cheng; Jin, Hua; He, Hong.
Título: Induction of defense-related enzymes in patchouli inoculated with virulent Ralstonia solanacearum
Fonte: Electron. j. biotechnol;27:63-69, May. 2017. graf.
Idioma: en.
Projeto: National Natural Science Foundation of China; . Programs Foundation of Ministry of Education of China.
Resumo: Background: Defense-related anti-oxidative response is a vital defense mechanism of plants against pathogen invasion. Ralstonia solanacearum is an important phytopathogen. Bacterial wilt caused by R. solanacearum is the most destructive disease and causes severe losses in patchouli, an important aromatic and medicinal plant in Southeast Asia. The present study evaluated the defense response of patchouli inoculated with virulent R. solanacearum. Results: Results showed that the basic enzymatic activities differed not only between the leaves and stems but also between the upper and lower parts of the same organ of patchouli. POD, SOD, PPO, and PAL enzymatic activities were significantly elevated in leaves and stems from patchouli inoculated with R. solanacearum compared to those in control. The variation magnitude and rate of POD, PPO, and PAL activities were more obvious than those of SOD in patchouli inoculated with R. solanacearum. PAGE isoenzymatic analysis showed that there were one new POD band and two new SOD bands elicited, and at least two isoformic POD bands and two SOD bands were observably intensified compared to the corresponding control. Conclusion: Our results suggest that not only defense-related enzymatic activities were elevated but also the new isoenzymatic isoforms were induced in patchouli inoculated with R. solanacearum.
Descritores: Ralstonia solanacearum/patogenicidade
Pogostemon/enzimologia
Pogostemon/microbiologia
-Fenilalanina Amônia-Liase/metabolismo
Superóxido Dismutase/metabolismo
Virulência
Catecol Oxidase/metabolismo
Peroxidase/metabolismo
Ralstonia solanacearum/fisiologia
Eletroforese em Gel de Poliacrilamida
Enzimas/imunologia
Enzimas/metabolismo
Eletroforese em Gel de Poliacrilamida Nativa
Pogostemon/imunologia
Antioxidantes
Responsável: CL1.1 - Biblioteca Central


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Texto completo SciELO Brasil
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Id: lil-755833
Autor: Itako, Adriana Terumi; Tolentino Júnior, João Batista; Silva Júnior, Tadeu Antônio Fernandes da; Soman, José Marcelo; Maringoni, Antonio Carlos.
Título: Chemical products induce resistance to Xanthomonas perforans in tomato
Fonte: Braz. j. microbiol;46(3):701-706, July-Sept. 2015. tab.
Idioma: en.
Projeto: FAPESP (The State of Sao Paulo Research Foundation); . National Council for the Improvement of Higher Education (CAPES).
Resumo:

The bacterial spot of tomato, caused by Xanthomonas spp., is a very important disease, especially in the hot and humid periods of the year. The chemical control of the disease has not been very effective for a number of reasons. This study aimed to evaluate, under greenhouse conditions, the efficacy of leaf-spraying chemicals (acibenzolar-S-methyl (ASM) (0.025 g.L−1), fluazinam (0.25 g.L−1), pyraclostrobin (0.08 g.L−1), pyraclostrobin + methiran (0.02 g.L−1 + 2.2 g.L−1), copper oxychloride (1.50 g.L−1), mancozeb + copper oxychloride (0.88 g.L−1 + 0.60 g.L−1), and oxytetracycline (0.40 g.L−1)) on control of bacterial spot. Tomatoes Santa Clara and Gisele cultivars were pulverized 3 days before inoculation with Xanthomonas perforans. The production of enzymes associated with resistance induction (peroxidase, polyphenol oxidase, phenylalanine ammonia-lyase, β-1,3-glucanase, and protease) was quantified from leaf samples collected 24 hours before and 24 hours after chemical spraying and at 1, 2, 4, 6, and 8 days after bacterial inoculation. All products tested controlled bacterial spot, but only ASM, pyraclostrobin, and pyraclostrobin + metiram increased the production of peroxidase in the leaves of the two tomato cultivars, and increased the production of polyphenol oxidase and β-1,3-glucanase in the Santa Clara cultivar.

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Descritores: Resistência à Doença/efeitos dos fármacos
Fungicidas Industriais/farmacologia
Lycopersicon esculentum/microbiologia
Doenças das Plantas/microbiologia
Xanthomonas/crescimento & desenvolvimento
-Catecol Oxidase/metabolismo
/metabolismo
GLUCAN 1,ABATTOIRS-BETA-GLUCOSIDASE/metabolismo
Lycopersicon esculentum/enzimologia
Lycopersicon esculentum/imunologia
Peptídeo Hidrolases/metabolismo
Peroxidase/metabolismo
Fenilalanina Amônia-Liase/metabolismo
Doenças das Plantas/imunologia
Xanthomonas/efeitos dos fármacos
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


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Id: lil-742930
Autor: GUIMARÃES, SS; MAZARO, SM; FREDDO, ÁR; WAGNER JÚNIOR, A.
Título: Potencial de preparados de cavalinha (Equisetum sp. ) na síntese de metabólitos de defesa em cotilédones de soja (Glycine max L. ) e o efeito sobre o crescimento de Rhizoctonia solani Kuhn, in vitro / Potential of horsetail (Equisetum sp. ) derivatives on the synthesis of defense metabolites using soybean ( Glycine max L. ) cotyledons and their effect on the in vitro growth of Rhizoctonia solani Kuhn
Fonte: Rev. bras. plantas med;17(1):143-149, Jan-Mar/2015. tab, graf.
Idioma: pt.
Resumo: Foram desenvolvidos dois experimentos com objetivo de avaliar o potencial de preparados de cavalinha (Equisetum sp.) na síntese de metabólitos de defesa em cotilédones de soja (Glycinemax L.) e o efeito sobre o crescimento de Rhizoctonia solani, in vitro. O delineamento experimental utilizado para os experimentos foi inteiramente casualizado em esquema fatorial 3x5 (formas de extração x concentrações), com quatro repetições. As formas de extração foram extrato alcoólico, infusão e maceração, nas concentrações de zero; 1; 10, 20 e 40%. No primeiro experimento foi avaliada a indução de compostos de defesa vegetal em cotilédones de soja em resposta aos derivados a base de cavalinha, sendo quantificada a atividade da enzima fenilalanina amônia-liase (FAL), via espectofotometria, a fitoalexina gliceolina, e o teor de fenóis totais. No segundo experimento, in vitro, a unidade experimental foi uma placa de Petri, sendo os preparados de cavalinha incorporados ao meio BDA (Batata-dextrose e Agar) e avaliado o crescimento micelial de R. Solani. Os preparados de extrato alcoólico, infusão e maceração de cavalinha apresentaram capacidade de indução das fitoalexinas gliceolinas em cotilédones de soja, bem como, ativaram o metabolismo de compostos fenólicos. Entre os preparados, o extrato alcoólico e a maceração, se sobressaem sobre a infusão. Os preparados de extrato alcoólico, infusão e maceração de cavalinha em todas as suas concentrações inibem o crescimento do fungo R. solani, in vitro. .

Two experiments were carried out in the Federal Technological University of Paraná - Dois Vizinhos Campus - with the aim to evaluate the potential of horsetail (Equisetum sp.) derivatives for the synthesis of defense metabolites in soybean (Glycine max L.) cotyledons and their effect on the in vitro growth of Rhizoctonia solani. The experimental design was completely randomized in a 3 x 5 factorial design (extraction form x concentration), with four replications. The extraction forms were alcoholic extract, infusion and maceration and the concentrations tested were zero, 1, 10, 20 and 40%. In the first experiment, we evaluated the induction of plant defense in soybean cotyledons as a response to horsetail derivatives through spectrophotometry according to phytoalexin glyceollin, phenylalanine ammonia lyase enzyme activity (PAL) and total phenols. In the second experiment, in vitro, the experimental unit was a Petri dish, and the horsetail derivatives were incorporated into medium culture (potato dextrose agar), and we evaluated the mycelial growth of R. solani. The alcoholic extract, infusion and maceration of horsetail derivatives presented phytoalexin glyceolin induction in soybean cotyledons, in addition to activating the metabolism of phenolic compounds. Among the derivatives, the alcoholic extract and the maceration form of extraction were superior in relation to the infusion. The alcoholic extract, infusion and maceration of horsetail derivatives inhibited the in vitro growth of R. solani in all concentrations.
Descritores: Cotilédone/classificação
Equisetum/fisiologia
Metabolismo
Rhizoctonia/classificação
Soja/classificação
-Fenilalanina Amônia-Liase/síntese química
Responsável: BR1.1 - BIREME


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Texto completo SciELO Chile
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Id: lil-309738
Autor: Herrig, Vanessa; Ferrarese, Maria de Lourdes L; Suzuki, Letícia S; Rodrigues, João Domingos; Ferrarese-Filho, Osvaldo.
Título: Peroxidase and phenylalanine ammonia-lyase activities, phenolic acid contents, and allelochemicals-inhibited root growth of soybean
Fonte: Biol. Res;35(1):59-66, 2002. tab, graf.
Idioma: en.
Resumo: The influence of the allelochemicals ferulic (FA) and vanillic (VA) acids on peroxidase (POD, EC 1.11.1.7) and phenylalanine ammonia-lyase (PAL, EC 4.3.1.5) activities and their relationships with phenolic acid (PhAs) contents and root growth of soybean (Glycine max (L.) Merr.) were examined. Three-day-old seedlings were cultivated in nutrient solution containing FA or VA (0.1 to 1 mM) for 48 h. Both compounds (at 0.5 and 1 mM) decreased root length (RL), fresh weight (FW) and dry weight (DW) and increased PhAs contents. At 0.5 and 1 mM, FA increased soluble POD activity (18% and 47%, respectively) and cell wall (CW)-bound POD activity (61% and 34%), while VA increased soluble POD activity (33% and 17%) but did not affect CW-bound POD activity. At 1 mM, FA increased (82%) while VA reduced (32%) PAL activities. The results are discussed on the basis of the role of these compounds on phenylpropanoid metabolism and root growth and suggest that the effects caused on POD and PAL activities are some of the many mechanisms by which allelochemicals influence plant growth.
Descritores: Ácidos Cumáricos
Hidroxibenzoatos
Peroxidase
Fenilalanina Amônia-Liase
Raízes de Plantas
Soja
Ácido Vanílico
-Peroxidase
Fenilalanina Amônia-Liase
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


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Texto completo SciELO Chile
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Id: lil-288332
Autor: Ortega, Ximena; Pérez, Luz M.
Título: Participation of the phosphoinositide metabolism in the hypersensitive response of Citrus limon against Alternaria alternata
Fonte: Biol. Res;34(1):43-50, 2001. graf, tab.
Idioma: en.
Resumo: Lemon seedlings inoculated with Alternaria alternata develop a hypersensitive response (HR) that includes the induction of Phenylalanine ammonia-lyase (PAL, E. C. 4.3.1.5) and the synthesis of scoparone. The signal transduction pathway involved in the development of this response is unknown. We used several inhibitors of the Phosphoinositide (PI) animal system to study a possible role of Inositol-1,4,5-triphosphate (IP3) in the transduction of the fungal conidia signal in Citrus limon. The HR was only partially inhibited by EGTA, suggesting that not only external but internal calcium as well are necessary for a complete development of the HR. In this plant system, Alternaria alternata induced an early accumulation of the second messenger IP3. When lemon seedlings were watered long term with LiCl, an inhibitor of the phosphoinositide cycle, the IP3 production was reduced, and the LiCl-watered plants could neither induce PAL nor synthesize scoparone in response to fungal conidia. Furthermore, neomycin, a Phospholipase C (PLC, E. C. 3.1.4.3) inhibitor, also inhibited PAL induction and scoparone synthesis in response to A. alternata. These results suggest that IP3 could be involved in the signal transduction pathway for the development of the HR of Citrus limon against A. alternata.
Descritores: Alternaria/patogenicidade
Citrus/fisiologia
Citrus/virologia
Fosfatidilinositóis/metabolismo
Transdução de Sinais
-Cafeína/farmacologia
Cálcio/farmacologia
Cumarínicos/antagonistas & inibidores
Cumarínicos/metabolismo
Fibrinolíticos/farmacologia
Heparina/farmacologia
Inositol 1,4,5-Trifosfato/fisiologia
Neomicina/farmacologia
Fenilalanina Amônia-Liase/metabolismo
Fosfatidilinositóis/antagonistas & inibidores
Inibidores de Fosfodiesterase/farmacologia
Inibidores da Síntese de Proteínas/farmacologia
Sementes
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME



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