Base de dados : LILACS
Pesquisa : D08.811.520.241.300.150.100 [Categoria DeCS]
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Id: biblio-973080
Autor: Facio, María Laura; De Rosa, Marcelo; Garlati, Claudia; Meller, Teresa; Colin, Laura; Alejandre, Mariel; Bresciani, Pablo; Brodsky, Andres.
Título: Hemoglobinuria paroxística nocturna. el rol de la anhidrasa carbónica-i urinaria en la hemólisis intravascular / Peroxysmal nocturnal hemoglobinuria: the role of urinary carbonic anhydrase-I in intravascular haemolysis
Fonte: Rev. Asoc. Méd. Argent;130(3):12-21, sept. 2017. ilus, tab.
Idioma: es.
Resumo: La Hemoglobinuria Paroxística Nocturna (HPN) se caracteriza por hemólisis intravascular crónica mediada por complemento. Cuando se produce la hemolisis se libera a circulación Anhidrasa Carbónica- I (AC-I), una enzima que se halla en alta concentración en el eritrocito y por su bajo peso molecular filtra por el glomérulo. El objetivo del presente trabajo fue detectar la excreción de la AC-I en orina de pacientes con HPN por Electroforesis Bidimensional de Utilidad Clínica (2D UC), y compararla con otras causas de hemólisis, de origen renal y postrenal. Se evaluaron 8 pacientes con HPN sin tratamiento con eculizumab un inhibidor del C5 del complemento, y 5 de ellos postratamiento, 12 orinas de pacientes con nefritis lúpica y 10 orinas de pacientes con hemólisis postrenal. La AC-I puede estar presente en la orina, en los tres grupos, sin embargo la relación AC-I/Hemoglobina en la hemólisis intravascular está invertida en comparación con la hemolisis glomerular y post-renal. Los pacientes con HPN tratados con eculizumab no presentan AC-I, y sería de utilidad en el seguimiento de los pacientes tratados con el inhibidor del C5, para evidenciar posibles escapes hemolíticos.

Paroxysmal Nocturnal Hemoglobinuria (PNH) is characterized by chronic complement mediated haemolysis. In these conditions it might be expected that carbonic anhydrase-I (AC-I) would be liberated into the plasma and excreted in the urine, by its high concentration in the erythrocyte and low molecular weight. The objective of the present study was to detect the urinary excretion of AC-I from patients with PNH by wodimensional clinical utility electrophoresis (2D UC) and to compare it with other causes of renal and post-renal haemolysis. We evaluated 8 patients with PNH without eculizumab, a complement C5 inhibitor, 5 of them posttreatment, 12 urine of patients with lupus nephritis and 10 urine of patients with post-renal hemolysis. AC-I may be present in the urine, in all three groups, however, the AC-I/Haemoglobin ratio in intravascular haemolysis is reversed compared to glomerular and post-renal haemolysis. Patients with PNH treated with eculizumab do not have AC-I and would be useful in monitoring patients treated with the C5 inhibitor to evidence possible haemolytic leaks.
Descritores: Hemoglobinúria Paroxística/urina
Anidrase Carbônica I/metabolismo
Anidrase Carbônica I/urina
Hemólise
-Hemoglobinúria Paroxística/tratamento farmacológico
Eletroforese/métodos
Urinálise/métodos
Lúpus Eritematoso Sistêmico/urina
Hematúria/urina
Anticorpos Monoclonais Humanizados/uso terapêutico
Limites: Humanos
Responsável: AR1.1 - Biblioteca Rafael Herrera Vegas


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Id: lil-762908
Autor: Song, Y.R.; Wu, B.; Yang, Y.T.; Chen, J.; Zhang, L.J.; Zhang, Z.W.; Shi, H.Y.; Huang, C.L.; Pan, J.X.; Xie, P..
Título: Specific alterations in plasma proteins during depressed, manic, and euthymic states of bipolar disorder
Fonte: Braz. j. med. biol. res = Rev. bras. pesqui. méd. biol;48(11):973-982, Nov. 2015. tab, graf.
Idioma: en.
Projeto: National Key Scientific Program of China.
Resumo: Bipolar disorder (BD) is a common psychiatric mood disorder affecting more than 1-2% of the general population of different European countries. Unfortunately, there is no objective laboratory-based test to aid BD diagnosis or monitor its progression, and little is known about the molecular basis of BD. Here, we performed a comparative proteomic study to identify differentially expressed plasma proteins in various BD mood states (depressed BD, manic BD, and euthymic BD) relative to healthy controls. A total of 10 euthymic BD, 20 depressed BD, 15 manic BD, and 20 demographically matched healthy control subjects were recruited. Seven high-abundance proteins were immunodepleted in plasma samples from the 4 experimental groups, which were then subjected to proteome-wide expression profiling by two-dimensional electrophoresis and matrix-assisted laser desorption/ionization-time-of-flight/time-of-flight tandem mass spectrometry. Proteomic results were validated by immunoblotting and bioinformatically analyzed using MetaCore. From a total of 32 proteins identified with 1.5-fold changes in expression compared with healthy controls, 16 proteins were perturbed in BD independent of mood state, while 16 proteins were specifically associated with particular BD mood states. Two mood-independent differential proteins, apolipoprotein (Apo) A1 and Apo L1, suggest that BD pathophysiology may be associated with early perturbations in lipid metabolism. Moreover, down-regulation of one mood-dependent protein, carbonic anhydrase 1 (CA-1), suggests it may be involved in the pathophysiology of depressive episodes in BD. Thus, BD pathophysiology may be associated with early perturbations in lipid metabolism that are independent of mood state, while CA-1 may be involved in the pathophysiology of depressive episodes.
Descritores: Apolipoproteína A-I/sangue
Apolipoproteínas/sangue
Transtorno Bipolar/sangue
Anidrase Carbônica I/sangue
Transtornos do Metabolismo dos Lipídeos/metabolismo
Lipoproteínas HDL/sangue
Proteômica
-Transtorno Bipolar/complicações
Transtorno Bipolar/diagnóstico
Bases de Dados de Proteínas
Diagnóstico Diferencial
Progressão da Doença
Regulação para Baixo
Transtorno Depressivo Maior/diagnóstico
Eletroforese em Gel Bidimensional
Immunoblotting
Imunoprecipitação
Transtornos do Metabolismo dos Lipídeos/complicações
Espectrometria de Massas/métodos
Limites: Adolescente
Adulto
Feminino
Humanos
Masculino
Adulto Jovem
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME



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