Base de dados : LILACS
Pesquisa : D08.811.682 [Categoria DeCS]
Referências encontradas : 112 [refinar]
Mostrando: 1 .. 10   no formato [Detalhado]

página 1 de 12 ir para página                         

  1 / 112 LILACS  
              next record last record
seleciona
para imprimir
Fotocópia
Texto completo SciELO Brasil
Texto completo
Id: biblio-1055421
Autor: Enzyme Biotechnology LaboratoryLugani, Yogita; Kauldhar, Baljinder Singh; Enzyme Biotechnology LaboratoryKaur, Navneet; Enzyme Biotechnology LaboratorySooch, Balwinder Singh.
Título: Phenotypic Characterization and Synthesis of Extracellular Catecholase from a Newly Isolated Bacterium Pseudomonas sp. BSC-6
Fonte: Braz. arch. biol. technol;62:e19180360, 2019. tab, graf.
Idioma: en.
Resumo: Abstract Catecholase (EC 1.10.3.1), an oxidoreductase enzyme is a key member of polyphenol oxidase family which catalyze the degradation of catechol. This enzyme possesses vast applications in diverse areas and is found in bacteria, fungi, mushrooms, higher plants, arthropods, amphibians and mammals. Catechol, a phenolic compound, is used as a starting material in the synthesis of various industrial compounds such as inhibitors, antioxidants, pesticides etc. The release of this phenolic compound in the environment causes toxicity to both flora and fauna. In the present studies, emphasis has been laid on isolation, screening and characterization of catechol degrading bacterium coupled with synthesis of catecholase enzyme. Further, the selected isolated strain was phenotypically characterized and was found to be member of genus Pseudomonas. Among all the isolates, BSC-6 was found as best isolate with maximum extracellular catecholase activity of 152.32 IU/L obtained after scale up studies. The herein synthesized bacterial catecholase may be employed for wide applications particularly in bioremediation of phenol enriched polluted sites.
Descritores: Oxirredutases
Catecóis
Polifenóis
-Pseudomonas
Biodegradação Ambiental
Responsável: BR1.1 - BIREME


  2 / 112 LILACS  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo SciELO Brasil
Texto completo
Id: biblio-1287094
Autor: Bhat, Shreesha K; Joshi, Manjunath B; Vasishta, Sampara; Jagadale, Rajesh N; Biligiri, Setlur G; Coronado, Monika A; Arni, Raghuvir K; Satyamoorthy, Kapaettu.
Título: P-I metalloproteinases and L-amino acid oxidases from Bothrops species inhibit angiogenesis
Fonte: J. venom. anim. toxins incl. trop. dis;27:e20200180, 2021. tab, graf.
Idioma: en.
Projeto: DBT/In- Bz; . CAPES; . FAPESP; . MCTI/CNPq/DBT.
Resumo: Snake venoms are composed of pharmacologically active proteins that are evolutionarily diverse, stable and specific to targets. Hence, venoms have been explored as a source of bioactive molecules in treating numerous diseases. Recent evidences suggest that snake venom proteins may affect the formation of new blood vessels. Excessive angiogenesis has been implicated in several pathologies including tumours, diabetic retinopathy, arthritis, inter alia. In the present study, we have examined the effects of P-I metalloproteinases isolated from Bothrops moojeni (BmMP-1) and Bothrops atrox (BaMP-1) and L-amino acid oxidases (LAAO) isolated from B. moojeni (BmLAAO) and B. atrox (BaLAAO) on biochemical and functional aspects of angiogenesis. Methods: P-I metalloproteinases and LAAO were purified from venom by molecular size exclusion and ion-exchange chromatography and subsequently confirmed using mass spectrometry. The P-I metalloproteinases were characterized by azocaseinolytic, fibrinogenolytic and gelatinase activity and LAAO activity was assessed by enzyme activity on L-amino acids. Influence of these proteins on apoptosis and cell cycle in endothelial cells was analysed by flow cytometry. The angiogenic activity was determined by in vitro 3D spheroid assay, Matrigel tube forming assay, and in vivo agarose plug transformation in mice. Results: P-I metalloproteinases exhibited azocaseinolytic activity, cleaved α and partially β chain of fibrinogen, and displayed catalytic activity on gelatin. LAAO showed differential activity on L-amino acids. Flow cytometry analysis indicated that both P-I metalloproteinases and LAAO arrested the cells in G0/G1 phase and further induced both necrosis and apoptosis in endothelial cells. In vitro, P-I metalloproteinases and LAAO exhibited significant anti-angiogenic properties in 3D spheroid and Matrigel models by reducing sprout outgrowth and tube formation. Using agarose plug transplants in mice harbouring P-I metalloproteinases and LAAO we demonstrated a marked disruption of vasculature at the periphery. Conclusion: Our research suggests that P-I metalloproteinases and LAAO exhibit anti-angiogenic properties in vitro and in vivo.(AU)
Descritores: Oxirredutases
Bothrops/fisiologia
Inibidores da Angiogênese
Venenos de Crotalídeos
Metaloproteases
Limites: Animais
Responsável: BR68.1 - Biblioteca Virginie Buff D'Ápice


  3 / 112 LILACS  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo SciELO Brasil
Texto completo
Id: biblio-1090608
Autor: Busato Júnior, Wilson F. S.
Título: Use of 5α-reductase inhibitor and delay in prostate cancer diagnosis and treatment
Fonte: Int. braz. j. urol;46(3):456-458, May-June 2020.
Idioma: en.
Descritores: Neoplasias da Próstata/tratamento farmacológico
Inibidores Enzimáticos/uso terapêutico
Inibidores de 5-alfa Redutase/uso terapêutico
-Oxirredutases
Neoplasias da Próstata/diagnóstico
Neoplasias da Próstata/metabolismo
Limites: Humanos
Masculino
Tipo de Publ: Editorial
Responsável: BR1.1 - BIREME


  4 / 112 LILACS  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo SciELO Brasil
Texto completo
Id: biblio-1041420
Autor: Miranda, Silvana Spindola de; Almeida, Isabela Neves de; Lopes, Maria Luiza; Figueiredo, Jamilly dos Reis de; Figueredo, Lida Jouca de Assis; Kritski, Afrânio Lineu; Carvalho, Wânia da Silva; Mansur, Maria de Fátima Filardi Oliveira.
Título: Evaluation of the commercial kit SIRE nitratase for detecting resistant mycobacterium tuberculosis in Brazil
Fonte: Rev. Soc. Bras. Med. Trop;50(4):550-553, July-Aug. 2017. tab.
Idioma: en.
Projeto: Minas Gerais State Research Support Foundation; . Conselho Nacional de Desenvolvimento Científico e Tecnológico.
Resumo: Abstract INTRODUCTION: This study aimed to evaluate a new commercial kit, Kit SIRE Nitratase-PlastLabor, for testing the drug susceptibility of clinical Mycobacterium tuberculosis isolates. METHODS: The accuracy of the Kit SIRE Nitratase was evaluated by examining the susceptibility (streptomycin, isoniazid, rifampicin, and ethambutol) of 40 M. tuberculosis isolates, using the proportion method with Lowenstein-Jensen medium or the BACTEC MGIT 960 system. RESULTS: The detection accuracy for streptomycin, isoniazid, rifampicin, and ethambutol was 95%, 97.5%, 100%, and 80%, respectively. CONCLUSIONS: The exceptional accuracy demonstrated by Kit SIRE Nitratase for isoniazid and rifampicin makes the kit an attractive option for screening M. tuberculosis strain resistance.
Descritores: Oxirredutases/farmacologia
Testes de Sensibilidade Microbiana/métodos
Antibióticos Antituberculose/farmacologia
Mycobacterium tuberculosis/efeitos dos fármacos
-Rifampina/farmacologia
Estreptomicina/farmacologia
Reprodutibilidade dos Testes
Farmacorresistência Bacteriana
Ensaios Enzimáticos Clínicos/métodos
Etambutol/farmacologia
Isoniazida/farmacologia
Mycobacterium tuberculosis/isolamento & purificação
Limites: Humanos
Responsável: BR1.1 - BIREME


  5 / 112 LILACS  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo SciELO Brasil
Texto completo
Id: biblio-1153298
Autor: Hooda, Vikas; Verma, Neelam; Gahlaut, Anjum; Gothwal, Ashish.
Título: Reusable Enzymatic Strip for Detection of Arsenic
Fonte: Braz. arch. biol. technol;64:e21200132, 2021. tab, graf.
Idioma: en.
Resumo: HIGHLIGHTS Arsenic is considered as one of the highly hazardous elements in the environment and a serious carcinogen for the human health. An enzymatic method has been described by using arsenite oxidase for arsenic detection. Residual activity of the immobilized enzyme was 43% of the initial activity after being recycled 10 times.

Abstract Arsenic is considered as one of the highly hazardous elements in the environment and a serious carcinogen for the human health. More attention has taken towards the arsenic due to its presence in ground water in India, China, Bangladesh, Inner Mongolia and several other regions of the world. It's been a challenge to remove arsenic due to the lack of its efficient detection approach in the complicated environmental matrix. The proposed method describes an enzymatic method for arsenic determination using arsenite oxidase, which catalyzes the oxidation of arsenite to arsenate. Hence, a colorimetric PVC strip with immobilized arsenite oxidase has been developed to detect the arsenic concentration and also having potential for the field-testing. The influence of the optimal conditions i.e. pH, temperature, storage stability, and reusability of free and immobilized enzyme were evaluated and compared. The results have shown that the stabilities were significantly enhanced compared with free counterpart. Residual activity of the immobilized enzyme was 43% of the initial activity after being recycled 10 times. We approve that this novel low cost immobilized carrier presents a new approach in large scale applications and expected to act as a model for establishment of indigenous arsenic sensor in miniature form.
Descritores: Arsênio/análise
Cloreto de Polivinila/análise
Poluentes Químicos da Água/análise
Água Subterrânea/análise
Enzimas Imobilizadas/análise
-Oxirredutases
Biodegradação Ambiental
Limites: Humanos
Responsável: BR1.1 - BIREME


  6 / 112 LILACS  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Raw, Isaias
Id: lil-179709
Autor: Pereira, Alexandre; Pereira, Aparecida Santo Pietro; Raw, Isaias.
Título: A rapid method to evaluate the bactericidal activity of hiperimmune serum against Vibrio cholerae 01 using oxidase test
Fonte: Rev. farm. bioquim. Univ. Säo Paulo;32(1):11-3, 1996. tab, graf.
Idioma: en.
Resumo: Desenvolveu-se ensaio rapido para medir o efeito de soros imunes contra o Vibrio cholerae 01, tendo-se por base a atividade de oxidase de culturas de bacterias em placas de ELISA
Descritores: Oxirredutases
Vibrio cholerae/efeitos dos fármacos
Teste Bactericida do Soro
Soros Imunes/análise
Soros Imunes/farmacologia
-Ensaio de Imunoadsorção Enzimática
Tempo de Reação
Limites: Animais
Coelhos
Responsável: BR40.1 - DBD - Divisão de Biblioteca e Documentacão do Conjunto das Químicas


  7 / 112 LILACS  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Id: lil-394923
Autor: Ramos, Rui Fernando.
Título: Marcadores séricos de necrose miocárdica / Serum cardiac markers of myocardial necrosis
Fonte: Rev. Soc. Cardiol. Estado de Säo Paulo;11(4):751-761, jul.-ago. 2001. tab.
Idioma: pt.
Resumo: O tratamento atual da síndrome coronariana aguda requer decisões rápidas. Os pacientes devem ser triados rápida e adequadamente, e liberados precocemente e com segurança. Os critérios diagnósticos de infarto agudo do miocárdio foram estabelecidos pela Organização Mundial da Saúde, pela presença de dois dos seguintes critérios: história e dor característica para isquemia, alterações eletrocardiográficas típicas, e elevação e normalização das enzimas cardíacas. Nos últimos trinta anos, a isoenzima CK-MB atividade tem sido o marcador padrão para esse diagnóstico, embora não seja específico para o diagnóstico. Novos métodos automatizados com testes imunológicos que utilizam anticorpos monoclonais para CK-MB massa, isoformas da CK-MB, mioglobina e troponina I e T foram introduzidos como possíveis substitutos da CK-MB atividade. Esses novos marcadores são mais sensíveis e específicos e podem detectar a presença de lesões mínimas do miocárdio em pacientes com dor torácica. Estudos recentes, que avaliaram o prognóstico desses pacientes, demonstraram que os portadores de troponina positiva evoluem com risco maior de óbito a curto e médio prazos. O uso desses marcadores pode, também, identificar a terapia mais adequada para essa população. A utilização da razão pré e pós-terapia fibrinolítica é um método eficaz para diagnóstico de reperfusão miocárdica. Este artigo examinará o papel das enzimas e proteínas cardíacas no diagnóstico bioquímico do infarto agudo do miocárdio, seu valor prognóstico, incluindo a creatina cinase, suas isoenzimas e isoformas, a desidrogenase láctica, a mioglobina e as troponinas T e I.
Descritores: Creatina Quinase
Isoenzimas
Infarto do Miocárdio/diagnóstico
Isoformas de Proteínas/fisiologia
Isoformas de Proteínas/história
Isoformas de Proteínas
-Oxirredutases
Troponina
Diabetes Mellitus
Mioglobina
Biomarcadores
Reperfusão Miocárdica
Limites: Humanos
Adulto
Idoso
Tipo de Publ: Revisão
Responsável: BR44.1 - Serviço de Biblioteca, Documentação Científica e Didática Prof. Dr. Luiz Venere Décourt


  8 / 112 LILACS  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo SciELO Brasil
Texto completo
Id: biblio-949365
Autor: Barlas, Aziz Mutlu; Kuru, Serdar; Kismet, Kemal; Cavusoglu, Turgut; Bag, Yusuf Murat; Senes, Mehmet; Cihan, Neslihan; Celepli, Pinar; Unal, Yilmaz; Hucumenoglu, Sema.
Título: Rectal application of argan oil improves healing of colorectal anastomosis in rats
Fonte: Acta cir. bras;33(7):565-576, July 2018. tab, graf.
Idioma: en.
Resumo: Abstract Purpose: To investigate the possible effects of argan oil on the healing of colorectal anastomoses. Methods: I n Group 1 (sham), laparotomy was performed and the colon was mobilized. In the control (Group 2) and argan oil (Group 3) groups, colonic resection and anastomosis were applied. To the control and sham groups, 2 mL of 0.9% NaCl was administred rectally, and in the argan oil group, 2 mL/day argan oil was applied rectally for 7 days. Results: The mean bursting pressures of the argan oil and sham groups were significantly higher than the values in the control group. A significant difference was determined between the tissue hydroxyproline and prolidase levels of control group and other groups. Histopathologically, argan oil showed significant beneficial effects on colonic wound healing. In the argan oil and sham groups, the tissue malondialdehyde and fluorescent oxidation product levels were found to be lower and total sulfhydryl levels were higher than the control group. Conclusions: The rectally administered argan oil was observed to have significantly ameliorated wound healing parameters and exerted a significant antioxidant effect. This is the first study in the literature about the beneficial effects of argan oil on colorectal anastomoses.
Descritores: Reto/cirurgia
Cicatrização/efeitos dos fármacos
Óleos Vegetais/uso terapêutico
Colo/cirurgia
Anti-Inflamatórios/uso terapêutico
Antioxidantes/uso terapêutico
-Oxirredutases/análise
Reto/patologia
Espectrofotometria
Anastomose Cirúrgica
Distribuição Aleatória
Reprodutibilidade dos Testes
Colágeno/análise
Resultado do Tratamento
Ratos Wistar
Colo/patologia
Estresse Oxidativo/efeitos dos fármacos
Dipeptidases/análise
Ferida Cirúrgica/patologia
Ferida Cirúrgica/tratamento farmacológico
Hidroxiprolina/análise
Malondialdeído/análise
Limites: Animais
Feminino
Tipo de Publ: Estudo de Avaliação
Responsável: BR1.1 - BIREME


  9 / 112 LILACS  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
Texto completo
Id: biblio-1048848
Autor: Almeida, Fernando Araújo de; Mann, Renata Silva; Santos, Heloisa Oliveira dos; Pereira, Rucyan Walace; Blank, Arie Fitzgerald.
Título: Germination temperatures affect the physiological quality of seeds of lettuce cultivars / Qualidade fisiológica de sementes de alface sob diferentes temperaturas na germinação
Fonte: Biosci. j. (Online);35(4):1143-1152, july/aug. 2019. tab, ilus.
Idioma: en.
Resumo: The objective of this study was to evaluate the physiological quality allied to biochemical quality of lettuce seeds by germination and enzymes expression at 20, 25, 30, 35, 40 and 42ºC. Germination speed index and percentage of germination were estimated. Isoenzyme expressions were assessed by alcohol dehydrogenase (ADH), malate dehydrogenase (MDH), catalase (CAT), esterase (EST), pyruvate decarboxylase (PDC) and glutamate oxaloacetate transferase (GOT). The experiment consisted of a completely randomized design in a factorial scheme 4x6, with four cultivars and six different temperatures, with four replications. The highest germination and vigor were observed for cv. 'Everglades' at 35°C, which proved that this cultivar is thermotolerant. Catalase can be considered a genetic marker for the identification ofthermotolerant lettuce cultivars. Cultivar 'Everglades' has potential to be used in lettuce breeding programs aimed at cultivars tolerant to high temperatures during germination.

O objetivo deste estudo foi avaliar a qualidade fisiológica e bioquímica de sementes de alface por meio da germinação e expressão de enzimas a 20, 25, 30, 35, 40 e 42ºC. As variáveis velocidade de germinação e o índice de velocidade de germinação foram estimadas. As expressões das enzimas alcool desidrogenase (ADH), malato desidrogenase (MDH), catalase (CAT), esterase (EST), piruvate descarboxilase (PDC) e glutamato oxaloacetato transferase (GOT) foram avaliadas. Para análise dos genótipos foi empregado o delineamento inteiramente casualizado em esquema fatorial 4x6, testando quatro cultivares e seis diferentes temperaturas, com quatro repetições. A maior germinação e vigor foram observadas para a cv. 'Everglades' a 35°C, o que prova que esta cultivar é termotolerante. A catalase pode ser considerada um marcador para a identificação de cultivares de alface termotolerantes. A cultivar 'Everglades' tem potential para uso em programas de melhoramento visando tolerância à alta temperatura durante a germinação.
Descritores: Sementes
Catalase
Alface
Esterases
Termotolerância
Isoenzimas
-Oxirredutases
Responsável: BR396.1 - Biblioteca Central


  10 / 112 LILACS  
              first record previous record
seleciona
para imprimir
Texto completo
Texto completo
Id: lil-126889
Autor: Mastro, Nélida Lúcia del.
Título: Noçöes de química da radiaçäo em sistemas biológicos / Notions of chemistry radiation in biological systems.
Fonte: Säo Paulo; s.n; out. 1989. 17 p. (Publicaçäo IPEN, 276).
Idioma: pt.
Resumo: A presente publicaçäo examina alguns aspectos dos efeitos biológicos diretos e indiretos da radiaçäo: formaçäo de pares, radicais livres, ion superóxido de hidrogênio, radical hidroxila, oxigênio singleto junto aos mecanismos radioprotetores endógenos dos organismos e os meios em que pode ser incrementada a radiorresistência de sistemas bioquímicos
Descritores: Radioquímica
-Oxirredutases/química
Radioatividade
Água/química
Radicais Livres
Íons
Oxigênio/química
Proteção Radiológica
Responsável: BR1.1 - BIREME
BR1.1/1415.00



página 1 de 12 ir para página                         
   


Refinar a pesquisa
  Base de dados : Formulário avançado   

    Pesquisar no campo  
1  
2
3
 
           



Search engine: iAH v2.6 powered by WWWISIS

BIREME/OPAS/OMS - Centro Latino-Americano e do Caribe de Informação em Ciências da Saúde