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Pesquisa : D08.811.682.400 [Categoria DeCS]
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Id: biblio-1021461
Autor: Soo, Chiu-Shyan; Yap, Wai-Sum; Hon, Wei-Min; Ramli, Norhayati; Md Shah, Umi Kalsom; Phang, Lai-Yee.
Título: Co-production of hydrogen and ethanol by Escherichia coli SS1 and its recombinant
Fonte: Electron. j. biotechnol;30:64-70, nov. 2017. ilus, graf, tab.
Idioma: en.
Projeto: Fundamental Research Grant Scheme.
Resumo: Background: The development of a potential single culture that can co-produce hydrogen and ethanol is beneficial for industrial application. Strain improvement via molecular approach was proposed on hydrogen and ethanol co-producing bacterium, Escherichia coli SS1. Thus, the effect of additional copy of native hydrogenase gene hybC on hydrogen and ethanol co-production by E. coli SS1 was investigated. Results: Both E. coli SS1 and the recombinant hybC were subjected to fermentation using 10 g/L of glycerol at initial pH 7.5. Recombinant hybC had about 2-fold higher cell growth, 5.2-fold higher glycerol consumption rate and 3-fold higher ethanol productivity in comparison to wild-type SS1. Nevertheless, wild-type SS1 reported hydrogen yield of 0.57 mol/mol glycerol and ethanol yield of 0.88 mol/mol glycerol, which were 4- and 1.4-fold higher in comparison to recombinant hybC. Glucose fermentation was also conducted for comparison study. The performance of wild-type SS1 and recombinant hybC showed relatively similar results during glucose fermentation. Additional copy of hybC gene could manipulate the glycerol metabolic pathway of E. coli SS1 under slightly alkaline condition. Conclusions: HybC could improve glycerol consumption rate and ethanol productivity of E. coli despite lower hydrogen and ethanol yields. Higher glycerol consumption rate of recombinant hybC could be an advantage for bioconversion of glycerol into biofuels. This study could serve as a useful guidance for dissecting the role of hydrogenase in glycerol metabolism and future development of effective strain for biofuels production.
Descritores: Etanol/metabolismo
Escherichia coli/metabolismo
Hidrogênio/metabolismo
Hidrogenase/metabolismo
-Recombinação Genética
Biodegradação Ambiental
Meios de Cultura
Escherichia coli/enzimologia
Alcalinização
Fermentação
Glucose/metabolismo
Glicerol/metabolismo
Hidrogenase/genética
Responsável: CL1.1 - Biblioteca Central


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Id: biblio-1009654
Autor: Soo, Chiu-Shyan; Yap, Wai-Sum; Hon, Wei-Min; Ramli, Norhayati; Md Shah, Umi Kalsom; Phang, Lai-Yee.
Título: Improvement of hydrogen yield of ethanol-producing Escherichia coli recombinants in acidic conditions
Fonte: Electron. j. biotechnol;26:27-32, Mar. 2017. tab, ilus, graf.
Idioma: en.
Projeto: Fundamental Research Grant Scheme; . Science and Technology Research Grant.
Resumo: Background: An effective single culture with high glycerol consumption and hydrogen and ethanol coproduction yield is still in demand. A locally isolated glycerol-consuming Escherichia coli SS1 was found to produce lower hydrogen levels under optimized ethanol production conditions. Molecular approach was proposed to improve the hydrogen yield of E. coli SS1 while maintaining the ethanol yield, particularly in acidic conditions. Therefore, the effect of an additional copy of the native hydrogenase gene hycE and recombinant clostridial hydrogenase gene hydA on hydrogen production by E. coli SS1 at low pH was investigated. Results: Recombinant E. coli with an additional copy of hycE or clostridial hydA was used for fermentation using 10 g/L (108.7 mmol/L) of glycerol with an initial pH of 5.8. The recombinant E. coli with hycE and recombinant E. coli with hydA showed 41% and 20% higher hydrogen yield than wild-type SS1 (0.46 ± 0.01 mol/mol glycerol), respectively. The ethanol yield of recombinant E. coli with hycE (0.50 ± 0.02 mol/mol glycerol) was approximately 30% lower than that of wild-type SS1, whereas the ethanol yield of recombinant E. coli with hydA (0.68 ± 0.09 mol/mol glycerol) was comparable to that of wild-type SS1. Conclusions: Insertion of either hycE or hydA can improve the hydrogen yield with an initial pH of 5.8. The recombinant E. coli with hydA could retain ethanol yield despite high hydrogen production, suggesting that clostridial hydA has an advantage over the hycE gene in hydrogen and ethanol coproduction under acidic conditions. This study could serve as a useful guidance for the future development of an effective strain coproducing hydrogen and ethanol.
Descritores: Etanol/metabolismo
Escherichia coli/metabolismo
Hidrogênio/metabolismo
-Biotecnologia
Proteínas Recombinantes
Clostridium/genética
Clostridium/metabolismo
Escherichia coli/enzimologia
Escherichia coli/genética
Fermentação
Glicerol
Concentração de Íons de Hidrogênio
Hidrogenase/genética
Hidrogenase/metabolismo
Responsável: CL1.1 - Biblioteca Central



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