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Pesquisa : D08.811.682.494 [Categoria DeCS]
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Id: biblio-1041762
Autor: Kuhar, Francisco; Castiglia, Valeria C; Zamora, Juan C.
Título: Detección de manganeso peroxidasa y otras exoenzimas en 4 aislamientos de Geastrum (Geastrales) en cultivo puro / Detection of manganese peroxidase and other exoenzymes in four isolates of Geastrum (Geastrales) in pure culture
Fonte: Rev. argent. microbiol;48(4):274-278, dic. 2016. ilus, graf, tab.
Idioma: en.
Resumo: Knowledge regarding the enzymatic machinery of fungi is decisive to understand their ecological role. The species of the genus Geastrum are known to grow extremely slowly in pure culture, which makes it difficult to evaluate physiological parameters such as enzyme activity. Qualitative assays were performed on isolates of four species of this genus, showing evidence of laccase, cellulase, pectinase, amylase and lipase activity and suggesting that a wide range of carbon sources can be exploited by these species. For the first time in this genus, quantitative assays verified manganese peroxidase activity (up to 0.6 mU/g) in 30-day old cultures, as well as laccase, β-glycosidase and β-xylosidase activities.

El conocimiento de la maquinaria enzimática de un hongo es decisivo para entender su rol ecológico. Las especies del género Geastrum son conocidas por su crecimiento extremadamente lento en cultivos puros, lo que hace difícil la evaluación de parámetros fisiológicos como las actividades enzimáticas. Se realizaron ensayos cualitativos sobre aislamientos de 4 especies de este género, mostrando evidencias de actividades lacasa, celulasa, pectinasa, amilasa y lipasa, mostrando el amplio rango de fuentes de carbono que pueden ser explotadas por estas especies. Ensayos cuantitativos verificaron por primera vez en este género la actividad manganeso peroxidasa (hasta 0,6 mU/g) en cultivos de 30 días, así como también β-glucosidasa y β-xilosidasa.
Descritores: Fungos/enzimologia
-Xilosidases/isolamento & purificação
Biotransformação/fisiologia
Celulase/isolamento & purificação
Lacase/isolamento & purificação
Fungos/fisiologia
Lipase/isolamento & purificação
Tipo de Publ: Ensaio Clínico
Estudo de Avaliação
Responsável: AR635.1 - FCVyS - Servicio de Información y Documentación


  2 / 27 LILACS  
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Id: biblio-1178913
Autor: Chávez, Georgina; Estrada, Nelson; Gómez, José; Choque, Reynaldo; Crespo, Carla; Alvarez, María Teresa.
Título: Potencial de cepas fúngicas aisladas en el área de biotecnología fúngica. Primera parte: uso de hongos en biorremediación / Potential of fungal strains isolated in the area of ​​Fungal biotechnology. Part one: Use of fungi in bioremediation
Fonte: Con-ciencia (La Paz);1(1):85-91, oct. 2013.
Idioma: es.
Resumo: El potencial de las cepas fúngicas en biorremediación, que han sido aisladas en el Instituto de Investigaciones Fármaco Bioquímicas (IIFB), es bastante amplio, se han encontrado cepas que han sido usadas en investigaciones a nivel internacional, tal es el caso de Bjerkandera sp BOL 13, un hongo aislado en un efluente contaminado con aceite en Oruro, con capacidad de degradar varios compuestos hidrocarbonados aromáticos policíclicos. En otro extremo de la nación se logró aislar Galerina sp., un hongo productor de lacasas, enzima importante en los procesos de decoloración.

The potential of fungi strains in biorremdiation that have been isolated in the Intiture of Research Pharmaceutical and Buiochemical (IIFB) is broad, it have been found strains that have been used in research at international level, as Bjerkandera sp BOL 13, a fungi isolated in an oil contaminated effluent, able to degrade polycycloic aromatic hydrocarbons. In other side of the country Galerina sp. was isolated, a laccase producing fungi, an enzyme important in decolorizing processes. contaminado con aceite en Oruro, con capacidad de degradar varios compuestos hidrocarbonados aromáticos policíclicos.
Descritores: Lacase
-Pesquisa
Fungos
Hidrocarbonetos Aromáticos
Responsável: BO138.1 - Biblioteca Central


  3 / 27 LILACS  
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Id: biblio-1132271
Autor: Bettin, Fernanda; Rosa, Letícia Osório da; Montanari, Queli; Zaccaria, Simone; Dillon, Aldo José Pinheiro; Silveira, Mauricio Moura da.
Título: Influence of Oxygen Supply on Growth and Laccases Production by Pleurotus sajor-caju PS-2001 in Submerged Process
Fonte: Braz. arch. biol. technol;63:e20190015, 2020. tab, graf.
Idioma: en.
Resumo: Abstract (1) Background: Oxygen supply is an important parameter to be considered in submerged cultures. This study evaluated the influence of different conditions for dissolved oxygen (DO) concentration on laccases activities and growth of Pleurotus sajor-caju PS-2001 in submerged process in stirred-tank bioreactor. (2) Methods: Initially, three different conditions were tested: uncontrolled DO and minimum levels of 30% and 80% of saturation, with the pH controlled between 4.5 and 7.0. (3) Results: Best results were observed at 30% DO (26 U mL-1 of laccases at 96 h), whereas higher mycelial biomass was observed at 30% and 80% DO (above 4.5 g L-1). Four different conditions of DO (uncontrolled, 10%, 30% and 50% of saturation) were tested at pH 6.5, with higher laccases activity (80 U mL-1 at 66 h) and lower mycelial growth (1.36 g L-1 at 90 h) being achieved with DO of 30%. In this test, the highest values for volumetric productivity and specific yield factor were determined. Under the different pH conditions tested, the production of laccases is favoured at DO concentration of 30% of saturation, while superior DO levels favours fungal growth. (4) Conclusion: The results indicate that dissolved oxygen concentration is a critical factor for the culture of P. sajor-caju PS-2001 and has important effects not only on laccases production but also on fungal growth.
Descritores: Oxigênio Dissolvido
Biomassa
Reatores Biológicos
Pleurotus/crescimento & desenvolvimento
Pleurotus/enzimologia
Lacase/biossíntese
Responsável: BR1.1 - BIREME


  4 / 27 LILACS  
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Id: biblio-1087520
Autor: Sarkar, Shrabana; Banerjee, Aparna; Chakraborty, Nibedita; Soren, Karuna; Chakraborty, Priyanka; Bandopadhyay, Rajib.
Título: Structural-functional analyses of textile dye degrading azoreductase, laccase and peroxidase: a comparative in silico study
Fonte: Electron. j. biotechnol;43:1-7, Jan. 2020. tab, graf, ilus.
Idioma: en.
Resumo: Background: Textile industry not only plays a vital role in our daily life but also a prominent factor in improving global economy. One of the environmental concern is it releases huge quantities of toxic dyes in the water leading to severe environmental pollution. Bacterial laccase and azoreductase successfully oxidize complex chemical structure of nitrogen group-containing azo dyes. Additionally, the presence of textile dye infuriates bacterial peroxidase to act as a dye degrading enzyme. Our present study deals with three textile dye degrading enzymes laccase, azoreductase, and peroxidase through analyzing their structural and functional properties using standard computational tools. Result: According to the comparative analysis of physicochemical characteristics, it was clear that laccase was mostly made up of basic amino acids whereas azoreductase and peroxidase both comprised of acidic amino acids. Higher aliphatic index ascertained the thermostability of all these three enzymes. Negative GRAVY value of the enzymes confirmed better water interaction of the enzymes. Instability index depicted that compared to laccase and preoxidase, azoreductase was more stable in nature. It was also observed that the three model proteins had more than 90% of total amino acids in the favored region of Ramachandran plot. Functional analysis revealed laccase as multicopper oxidase type enzyme and azoreductase as FMN dependent enzyme, while peroxidase consisted of α-ß barrel with additional haem group. Conclusion: Present study aims to provide knowledge on industrial dye degrading enzymes, choosing the suitable enzyme for industrial set up and to help in understanding the experimental laboratory requirements as well.
Descritores: Compostos Azo/metabolismo
Peroxidase/química
Lacase/química
NADH NADPH Oxirredutases/química
-Temperatura
Compostos Azo/química
Indústria Têxtil
Biodegradação Ambiental
Simulação por Computador
Estabilidade Enzimática
Peroxidase/metabolismo
Lactase/metabolismo
Corantes/metabolismo
NADH NADPH Oxirredutases/metabolismo
Tipo de Publ: Estudo Comparativo
Responsável: CL1.1 - Biblioteca Central


  5 / 27 LILACS  
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Id: biblio-951607
Autor: Chicatto, J A; Rainert, K T; Gonçalves, M J; Helm, C V; Altmajer-Vaz, D; Tavares, L B B.
Título: Decolorization of textile industry wastewater in solid state fermentation with Peach-Palm (Bactris gasipaes) residue / Decoloração de águas residuais da indústria têxtil em fermentação de estado sólido com resíduo de Pupunha (Bactris gasipaes)
Fonte: Braz. j. biol;78(4):718-727, Nov. 2018. tab, graf.
Idioma: en.
Projeto: MCTI.
Resumo: Abstract In this work we have assessed the decolorization of textile effluents throughout their treatment in a solid-state fermentation (SSF) system. SSF assays were conducted with peach-palm (Bactris gasipaes) residue using the white rot fungus Ganoderma lucidum EF 31. The influence of the dye concentration and of the amounts of peach-palm residue and liquid phase on both the discoloration efficiency and enzyme production was studied. According to our results, independently of experimental conditions employed, laccase was the main ligninolytic enzyme produced by G. lucidum. The highest laccase activity was obtained at very low effluent concentrations, suggesting the existence of an inhibitory effect of higher concentrations on fungal metabolism. The highest percentage of color removal was reached when 10 grams of peach palm residue was moistened with 60 mL of the final effluent. In control tests carried out with the synthetic dye Remazol Brilliant Blue R (RBBR) decolorization efficiencies about 20% higher than that achieved with the industrial effluent were achieved. The adsorption of RBBR on peach-palm residue was also investigated. Equilibrium tests showed that the adsorption of this dye followed both Langmuir and Freundlich isotherms. Hence, our experimental results indicate that peach-palm residue is suitable substrate for both laccase production and color removal in industrial effluents.

Resumo Neste trabalho, avaliamos a descoloração de efluentes têxteis durante seu tratamento em um sistema de fermentação em estado sólido (SSF). Os ensaios foram conduzidos com resíduo de pupunha (Bactris gasipaes) utilizando o fungo de podridão branca Ganoderma lucidum EF 31. A influência da concentração de corante, as quantidades de resíduo e da fase líquida foram estudadas tanto na eficiência de descoloração como na produção de enzima. De acordo com os resultados, independentemente das condições experimentais utilizadas, a lacase foi a principal enzima ligninolítica produzida por G. lucidum. A atividade de lacase mais elevada foi obtida em baixas concentrações de efluentes, sugerindo um efeito inibitório no metabolismo fúngico. A maior remoção de cor foi obtida com 10 gramas de resíduo da pupunha e 60 mL do efluente final. Nos ensaios de controle realizados com o corante sintético RBBR, foram atingidos cerca de 20% mais descoloração do que os obtidos com o efluente industrial. A adsorção de RBBR no resíduo de pupunha também foi investigada. Os testes de equilíbrio mostraram que a adsorção deste corante seguiu as isotermas de Langmuir e Freundlich. Assim, os resultados experimentais indicam que o resíduo de pupunha é um substrato adequado tanto para a produção de lacase quanto para a remoção de cor em efluentes industriais.
Descritores: Indústria Têxtil/métodos
Biodegradação Ambiental
Reishi/enzimologia
Arecaceae/química
Lacase/química
Águas Residuárias/química
-Antraquinonas
Cor
Adsorção
Corantes/química
Fermentação
Responsável: BR1.1 - BIREME


  6 / 27 LILACS  
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Id: biblio-974344
Autor: Hernández-Monjaraz, Wendy Susana; Caudillo-Pérez, César; Salazar-Sánchez, Pedro Ulises; Macías-Sánchez, Karla Lizbeth.
Título: Influence of iron and copper on the activity of laccases in Fusarium oxysporum f. sp. lycopersici
Fonte: Braz. j. microbiol;49(supl.1):269-275, 2018. tab, graf.
Idioma: en.
Projeto: Instituto Politécnico Nacional, Secretaría de Investigación y Posgrado.
Resumo: ABSTRACT Fusarium oxysporum f. sp. lycopersici is a phytopathogenic fungus that causes vascular wilt in tomato plants. In this work we analyze the influence of metal salts such as iron and copper sulphate, as well as that of bathophenanthrolinedisulfonic acid (iron chelator) and bathocuproinedisulfonic acid (copper chelator) on the activity of laccases in the intra (IF) and extracellular fractions (EF) of the wild-type and the non-pathogenic mutant strain (rho1::hyg) of F. oxysporum. The results show that laccase activity in the IF fraction of the wild and mutant strain increased with the addition of iron chelator (53.4 and 114.32%; respectively). With copper, it is observed that there is an inhibition of the activity with the addition of CuSO4 for the EF of the wild and mutant strain (reduction of 82 and 62.6%; respectively) and for the IF of the mutant strain (54.8%). With the copper chelator a less laccase activity in the IF of the mutant strain was observed (reduction of 53.9%). The results obtained suggest a different regulation of intracellular laccases in the mutant strain compared with the wild type in presence of CuSO4 and copper chelator which may be due to the mutation in the rho gene.
Descritores: Proteínas Fúngicas/metabolismo
Cobre/metabolismo
Lacase/metabolismo
Fusarium/enzimologia
Ferro/metabolismo
-Doenças das Plantas/microbiologia
Proteínas Fúngicas/genética
Proteínas Fúngicas/química
Lycopersicon esculentum/microbiologia
Lacase/genética
Lacase/química
Fusarium/genética
Fusarium/química
Responsável: BR1.1 - BIREME


  7 / 27 LILACS  
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Id: lil-780826
Autor: Fonseca, María Isabel; Tejerina, Marcos Raúl; Sawostjanik-Afanasiuk, Silvana Soledad; Giorgio, Ernesto Martin; Barchuk, Mónica Lucrecia; Zapata, Pedro Darío; Villalba, Laura Lidia.
Título: Preliminary studies of new strains of Trametes sp. from Argentina for laccase production ability
Fonte: Braz. j. microbiol;47(2):287-297, Apr.-June 2016. tab, graf.
Idioma: en.
Resumo: Abstract Oxidative enzymes secreted by white rot fungi can be applied in several technological processes within the paper industry, biofuel production and bioremediation. The discovery of native strains from the biodiverse Misiones (Argentina) forest can provide useful enzymes for biotechnological purposes. In this work, we evaluated the laccase and manganese peroxidase secretion abilities of four newly discovered strains of Trametes sp. that are native to Misiones. In addition, the copper response and optimal pH and temperature for laccase activity in culture supernatants were determined.The selected strains produced variable amounts of laccase and MnP; when Cu2+ was added, both enzymes were significantly increased. Zymograms showed that two isoenzymes were increased in all strains in the presence of Cu2+. Strain B showed the greatest response to Cu2+ addition, whereas strain A was more stable at the optimal temperature and pH. Strain A showed interesting potential for future biotechnological approaches due to the superior thermo-stability of its secreted enzymes.
Descritores: Proteínas Fúngicas/metabolismo
Lacase/metabolismo
Trametes/enzimologia
-Argentina
Temperatura
Estabilidade Enzimática
Proteínas Fúngicas/genética
Proteínas Fúngicas/química
Lacase/genética
Lacase/química
Trametes/isolamento & purificação
Trametes/genética
Responsável: BR1.1 - BIREME


  8 / 27 LILACS  
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Id: biblio-1015723
Autor: Wang, Jiayi; Lu, Lei; Feng, Fujuan.
Título: Combined strategies for improving production of a thermo-alkali stable laccase in Pichia pastoris
Fonte: Electron. j. biotechnol;28:7-13, July. 2017. tab, graf, ilus.
Idioma: en.
Projeto: National Natural Science Foundation of China; . Fundamental Research Funds for the Central Universities.
Resumo: Background: Laccases are copper-containing enzymes which have been used as green biocatalysts for many industrial processes. Although bacterial laccases have high stabilities which facilitate their application under harsh conditions, their activities and production yields are usually very low. In this work, we attempt to use a combinatorial strategy, including site-directed mutagenesis, codon and cultivation optimization, for improving the productivity of a thermo-alkali stable bacterial laccase in Pichia pastoris. Results: A D500G mutant of Bacillus licheniformis LS04 laccase, which was constructed by site-directed mutagenesis, demonstrated 2.1-fold higher activity when expressed in P. pastoris. The D500G variant retained similar catalytic characteristics to the wild-type laccase, and could efficiently decolorize synthetic dyes at alkaline conditions. Various cultivation factors such as medium components, pH and temperature were investigated for their effects on laccase expression. After cultivation optimization, a laccase activity of 347 ± 7 U/L was finally achieved for D500G after 3 d of induction, which was about 9.3 times higher than that of wild-type enzyme. The protein yield under the optimized conditions was about 59 mg/L for D500G. Conclusions: The productivity of the thermo-alkali stable laccase from B. licheniformis expressed in P. pastoris was significantly improved through the combination of site-directed mutagenesis and optimization of the cultivation process. The mutant enzyme retains good stability under high temperature and alkaline conditions, and is a good candidate for industrial application in dye decolorization.
Descritores: Pichia/metabolismo
Lacase/biossíntese
Lacase/genética
Bacillus licheniformis/enzimologia
-Temperatura
Leveduras
Estabilidade Enzimática
Catálise
Mutagênese
Lacase/metabolismo
Corantes/metabolismo
Concentração de Íons de Hidrogênio
Responsável: CL1.1 - Biblioteca Central


  9 / 27 LILACS  
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Id: biblio-999077
Autor: Teodoro, Thaís Silva; Oliveira, Fernanda de; Poffo, Caroline; Braga, Laís Pacenko; Arbigaus, Auriciane; Rampinelli, Jamile Rosa; Wisbeck, Elisabeth; Bonatti-Chaves, Mariane; Furlan, Sandra Aparecida.
Título: The influence of Tween 80 on laccase production by Pleurotus sajor-caju and the efficiency of crude enzyme broth in the removal of bisphenol-A / Influência do Tween 80 na produção de lacases de Pleurotus sajor-caju e eficiência do caldo enzimático bruto na remoção de bisfenol-A
Fonte: Arq. Inst. Biol;85:e1022017, 2018. tab, graf.
Idioma: en.
Projeto: CNPq; . FAP.
Resumo: Bisphenol-A is currently considered an environmental pollutant, capable of interfering in the endocrine system of organisms and causing alterations in its development and reproductive system. An alternative method to the chemical treatment of this pollutant has been the use of oxidative enzymes, especially laccases produced by fungi. In order to reduce production costs, agro-industrial waste can be used in the culture medium composition. Nonionic surfactants, which are only slightly toxic to biological membranes, can be applied, as well as Tween 80, to facilitate the excretion of these enzymes into the culture medium. The objectives of this work were: a) characterize the immersion water of banana straw used in the formulation of the culture medium; b) evaluate laccase production by Pleurotus sajor-caju in culture medium with and without addition of Tween 80, through shaken flasks; c) evaluate the efficiency of the crude enzyme broth in degrading bisphenol-A. The shaken flasks were incubated at 30°C for 12 days. The immersion water had a C:N ratio of 13.8, ash percentage of 28.6%, and pH close to neutrality. The addition of Tween 80 on the culture medium (7.5%, m/v) yielded laccase activity and productivity values equal to 3,016.47 U L-1 and 502.7 U L-1 day-1, respectively. These values were 50 and 33.5 times higher than those obtained in the culture medium without addition of Tween 80 for laccase activity and productivity, respectively. The crude enzyme broth degraded 100% of bisphenol-A after 48 hours, regardless of concentration (500, 750 and 1,000 mg L-1).(AU)

O bisfenol-A é considerado um poluente ambiental capaz de interferir no sistema endócrino dos organismos, ocasionando alterações em seu desenvolvimento e sistema de reprodução. Um método alternativo ao tratamento químico desse tipo de poluente tem sido a utilização de enzimas oxidativas, especialmente as lacases, produzidas por fungos. A fim de diminuir custos de produção, resíduos agroindustriais podem compor o meio de cultivo. Assim, surfactantes não iônicos e pouco tóxicos para as membranas biológicas, como o Tween 80, podem ser utilizados para facilitar a excreção dessas enzimas para o meio de cultivo. Os objetivos deste trabalho foram: caracterizar quimicamente o resíduo água de imersão de palha de bananeira, usado na formulação do meio de cultivo; avaliar a produção de lacase por Pleurotus sajor-caju em meio de cultivo líquido (frascos Erlenmeyer) com e sem adição de Tween 80; e avaliar a eficiência do caldo enzimático bruto em degradar bisfenol-A. Os frascos foram incubados a 30°C, por 12 dias. A água de imersão apresentou relação C:N 13,8, percentual de cinzas 28,6% e pH próximo da neutralidade. O cultivo adicionado de Tween 80 (7,5%, m/v) propiciou valores de atividade e produtividade em lacase iguais a 3.016,47 U L-1 e 502,7 U L-1 dia-1, respectivamente. Esses valores são 50 e 33,5 vezes maiores que os obtidos no cultivo sem adição de Tween 80, para atividade e produtividade em lacase, respectivamente. O caldo enzimático bruto degradou 100% do bisfenol-A após 48 horas, independentemente da concentração (500, 750 e 1.000 mg L-1).(AU)
Descritores: Polissorbatos
Tensoativos
Pleurotus
Sistema Endócrino
-Musa
Lacase
Poluentes Ambientais
Enzimas
Responsável: BR1942.1 - NID - Biblioteca - Núcleo de Informação e Documentação


  10 / 27 LILACS  
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Id: biblio-988465
Autor: Flórez Sampedro, Laura; Zapata, Wildeman; Orozco, Lina P; Mejía, Amanda I; Arboleda, Carolina; Rugeles, María T.
Título: In vitro anti-HIV-1 activity of the enzymatic extract enriched with laccase produced by the fungi Ganoderma sp. and Lentinus sp / Actividad anti-VIH-1 in vitro del extracto enzimático enriquecido con lacasa producido por los hongos Ganoderma sp. y Lentinus sp
Fonte: Vitae (Medellín);23(2):109-118, 2016. Ilustraciones.
Idioma: en.
Resumo: Background: Natural compounds are a good source for the development of antiretroviral drugs with low cytotoxicity. The laccase enzyme, produced by fungi of the genera Ganoderma sp. and Lentinus sp., inhibits the reverse transcriptase (RT) of the human immunodeficiency virus 1 (HIV-1), in cell-free models in vitro. Objetives: In this study we evaluated the anti-HIV-1 activity of the enzymatic extracts (EE) enriched with laccase, produced by two native species of fungi of the same genera in an in vitro cell culture model. Methods: The inhibition of viral replication was performed using the U373-MAGI cell line infected with recombinant viruses in the presence/absence of the EE and 48 hpi, the percentage of infected cells was evaluated by flow cytometry for green fluorescent protein ­GFP­ and ELISA for p24. The inhibition of the RT was determined by quantification of early and late products of reverse transcription using quantitative PCR. Results: The EEs from Ganoderma sp. and Lentinus sp. inhibited the replication of HIV-1 between 80 and 90% and decreased the production of early and late transcripts between 55,5%-91,3% and 82,1%-93,6% respectively. The EE from Lentinus sp. had the best selectivity index (SI: 8.3). Conclusions: These results suggest the potential anti-HIV-1 activity of the EE for the exploration of an alternative therapy against HIV-1 infection.

Antecedentes: Los compuestos naturales son una buena fuente para el desarrollo de fármacos antirretrovirales con baja citotoxicidad. La enzima lacasa, producida por hongos del género Ganoderma sp. y Lentinus sp., inhibe la transcriptasa reversa (TR) del virus de la inmunodeficiencia humana tipo 1 (VIH-1), en modelos in vitro, libres de células. Objetivos: En este estudio se evaluó la actividad anti-VIH-1 del extracto enzimático (EE) enriquecido con lacasa, producida por dos especies nativas de hongos de los mismos géneros en un modelo in vitro de cultivo celular. Métodos: La inhibición de la replicación viral se realizó usando la línea celular U373-MAGI infectada con virus recombinantes en la presencia/ ausencia del EE y 48 hpi, el porcentaje de células infectadas se evaluó mediante citometría de flujo para GFP y ELISA para p24. La inhibición de la TR se determinó mediante la cuantificación de los productos tempranos y tardíos de la transcripción reversa utilizando una PCR cuantitativa. Resultados: El EE de Ganoderma sp. y Lentinus sp. inhibió la replicación del VIH-1 entre el 80 y 90% y disminuyó la producción de transcriptos tempranos y tardíos entre el 55,5% -91,3% y 82,1% -93,6%, respectivamente. El EE de Lentinus sp. mostró el mejor índice de selectividad (IS: 8.3). Conclusiones: Estos resultados sugieren el potencial anti-VIH-1 del EE para la exploración de una terapia alternativa contra la infección por el VIH-1.
Descritores: Antivirais
HIV-1
-Produtos Biológicos
Lentinula
Ganoderma
Lacase
Limites: Humanos
Tipo de Publ: Artigo Clássico
Responsável: CO56.3 - Biblioteca



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