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Id: biblio-974939
Autor: Harada, Tomoyuki; Fink, Mitchell; Cruz Jr, Ruy J.
Título: Ethacrynic acid decreases expression of proinflammatory intestinal wall cytokines and ameliorates gastrointestinal stasis in murine postoperative ileus
Fonte: Clinics;73:e332, 2018. graf.
Idioma: en.
Resumo: OBJECTIVES: Several compounds characterized by an olefin linkage conjugated to a carbonyl group have anti-inflammatory properties. The diuretic ethacrynic acid (EA) is a compound of this type. Herein, we tested the hypothesis that ethacrynic acid can modulate the development of ileus after bowel manipulation. METHODS: Groups (n=9) of male C57Bl/6 mice underwent surgical manipulation of the small intestine using a pair of cotton-tipped applicators (MAN). Control animals (CONT) did not undergo any surgical intervention or receive treatment. MAN mice were pre- and post-treated with four intraperitoneal doses of phosphate buffered saline (PBS), EA1 (1mg/kg per dose), or EA10 (10mg/kg per dose). Gastrointestinal transit of non-absorbable FITC-labeled dextran was assessed by gavaging the mice with the tracer 24h after operation and assessing FD70 concentration 120 min later in the bowel contents from the stomach, 10 equally long segments of small intestine, cecum, and two equally long segments of colon. The geometric center for the tracer was calculated for each animal. Expression of interleukin-6 (IL-6) and inducible nitric oxide synthase (iNOS) transcripts in the ileal muscularis propria was assessed using semiquantitative reverse transcriptase-polymerase chain reaction. RESULTS: In control animals, the mean (±SE) geometric center for the transit marker was 9.89±0.47, whereas it was 4.59±0.59 for PBS-treated animals (p<0.05 vs CONT). The geometric center for pre- post treatment with low (1mg/kg) and high (10mg/kg) doses of ethacrynic acid were 7.23±0.97 and 5.15±0.57, respectively. Compared to PBS, treatment with ethacrynic acid (1mg/kg) significantly decreased manipulation-induced IL-6 and iNOS mRNA expression in the wall of the small bowel. CONCLUSIONS: Pre- and post-treatment with ethacrynic acid ameliorates ileus and modulates inflammation in the gut wall induced by bowel manipulation.
Descritores: Trânsito Gastrointestinal/efeitos dos fármacos
Interleucina-6/antagonistas & inibidores
Mediadores da Inflamação/antagonistas & inibidores
Íleus/patologia
Óxido Nítrico Sintase Tipo II/antagonistas & inibidores
Ácido Etacrínico/farmacologia
Intestino Delgado/efeitos dos fármacos
-Complicações Pós-Operatórias
Reação em Cadeia da Polimerase Via Transcriptase Reversa
Íleus/cirurgia
Modelos Animais de Doenças
Intestino Delgado/patologia
Camundongos Endogâmicos C57BL
Limites: Animais
Masculino
Camundongos
Responsável: BR1.1 - BIREME


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Id: biblio-896995
Autor: Fitri, Loeki Enggar; Rosmarwati, Ervina; Rizky, Yesita; Budiarti, Niniek; Samsu, Nur; Mintaroem, Karyono.
Título: Strong renal expression of heat shock protein 70, high mobility group box 1, inducible nitric oxide synthase, and nitrotyrosine in mice model of severe malaria
Fonte: Rev. Soc. Bras. Med. Trop;50(4):489-498, July-Aug. 2017. graf.
Idioma: en.
Resumo: Abstract INTRODUCTION Renal damage is a consequence of severe malaria, and is generally caused by sequestration of Plasmodium falciparum -infected erythrocytes in the renal microcirculation, which leads to obstruction, hypoxia, and ischemia. This triggers high mobility group box 1 (HMGB1) to send a danger signal through toll-like receptors 2 and 4. This signal up-regulates inducible nitric oxide (iNOS) and nitrotyrosine to re-perfuse the tissue, and also increases heat shock protein 70 (HSP70) expression. As no study has examined the involvement of intracellular secondary molecules in this setting, the present study compared the renal expressions of HSP70, HMGB1, iNOS, and nitrotyrosine between mice suffered from severe malaria and normal mice. METHODS C57BL/6 mice were divided into an infected group (intraperitoneal injection of 10 6 P. berghei ANKA) and a non-infected group. Renal damage was evaluated using hematoxylin eosin staining, and immunohistochemistry was used to evaluate the expressions of HSP70, HMGB1, iNOS, and nitrotyrosine. RESULTS Significant inter-group differences were observed in the renal expressions of HSP70, HMGB1, and iNOS (p=0.000, Mann-Whitney test), as well as nitrotyrosine (p=0.000, independent t test). The expressions of HSP70 and HMGB1 were strongly correlated (p=0.000, R=1.000). No correlations were observed between iNOS and HMGB, HMGB1 and nitrotyrosine, HSP70 and nitrotyrosine, or iNOS and nitrotyrosine. CONCLUSIONS It appears that HMGB1, HSP70, iNOS, and nitrotyrosine play roles in the renal damage that is observed in mice with severe malaria. Only HSP70 expression is strongly correlated with the expression of HMGB1.
Descritores: Tirosina/antagonistas & inibidores
Proteínas de Choque Térmico HSP70/metabolismo
Proteína HMGB1/metabolismo
Óxido Nítrico Sintase Tipo II/metabolismo
Injúria Renal Aguda/parasitologia
Malária/complicações
Malária/metabolismo
-Tirosina/metabolismo
Índice de Gravidade de Doença
Modelos Animais de Doenças
Camundongos
Camundongos Endogâmicos C57BL
Limites: Animais
Feminino
Responsável: BR1.1 - BIREME


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Id: biblio-950765
Autor: Park, Hee-Sook; Choi, Hye-Young; Kim, Gun-Hee.
Título: Preventive effect of Ligularia fischeri on inhibition of nitric oxide in lipopolysaccharide-stimulated RAW 264. 7 macrophages depending on cooking method
Fonte: Biol. Res;47:1-6, 2014. graf, tab.
Idioma: en.
Projeto: Globalization of Korean Foods R& D program; . Ministry of Agriculture, Food, and Rural Affairs, Republic of Korea; . National Research Foundation of Korea; . Ministry of Education. Basic Science Research Program.
Resumo: BACKGROUND: Ligularia fischeri (common name Gomchwi) is known for its pharmaceutical properties and used in the treatment of jaundice, scarlet-fever, rheumatoidal arthritis, and hepatic diseases; however, little is known about its anti-inflammatory effect. In this study the influence of blanching and pan-frying on the anti-inflammatory activity of Ligularia fischeri (LF) was evaluated. RESULTS: Fresh LF and cooked LF showed no significant effect on the viability of macrophages after 24 h incubation. Fresh LF was found to be the most potent inhibitor of nitric oxide (NO) production at 100 µg/ml, while pan-fried LF showed little inhibitory effect on lipoloysaccharide (LPS) stimulated murine machrophage RAW264.7 cells. In contrast with its effect on NO production, pan-fried LF showed significant attenuation of the expression of inducible nitiric oxide synthase (iNOS) compared with fresh LF. In the cooking method of LF, PGE2 production was not affected in the LPS-induced RAW 264.7 cells. In LPS-induced RAW 264.7 cells, pretreatment by fresh and cooked LF increased COX2 mRNA expression. The 3-O-caffeoylquinic acid content of blanching and pan-frying LF increased by 4.92 and 9.7 fold with blanching and pan-frying respectively in comparison with uncooked LF. CONCLUSIONS: Regardless of the cooking method, Ligularia fischeri exhibited potent inhibition of NO production through expression of iNOS in LPS-induced RAW264.7 cells.
Descritores: Culinária/métodos
Asteraceae/química
Preparações de Plantas/farmacologia
Óxido Nítrico Sintase Tipo II/metabolismo
Macrófagos/efeitos dos fármacos
Óxido Nítrico/biossíntese
-Ácido Quínico/análise
Ácido Quínico/análogos & derivados
Ácido Quínico/classificação
RNA Mensageiro/efeitos dos fármacos
RNA Mensageiro/metabolismo
Dinoprostona/análise
Dinoprostona/biossíntese
Sobrevivência Celular
Lipopolissacarídeos
Cromatografia Líquida de Alta Pressão
Asteraceae/classificação
Ciclo-Oxigenase 2/análise
Ciclo-Oxigenase 2/metabolismo
Células RAW 264.7
Temperatura Alta
Macrófagos/fisiologia
Anti-Inflamatórios/farmacologia
Limites: Animais
Camundongos
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: CL1.1 - Biblioteca Central


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Vassallo, Dalton Valentim
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Id: biblio-838702
Autor: Faria, Thaís de Oliveira; Angeli, Jhuli Keli; Mello, Luiz Guilherme Marchesi; Pinto, Gustavo Costa; Stefanon, Ivanita; Vassallo, Dalton Valentim; Lizardo, Juliana Hott de Fúcio.
Título: A Single Resistance Exercise Session Improves Aortic Endothelial Function in Hypertensive Rats / Uma Única Sessão de Exercício Resistido Melhora a Função Endotelial Aórtica em Ratos Hipertensos
Fonte: Arq. bras. cardiol;108(3):228-236, Mar. 2017. tab, graf.
Idioma: en.
Resumo: Abstract Background: Physical exercise is an important tool for the improvement of endothelial function. Objective: To assess the effects of acute dynamic resistance exercise on the endothelial function of spontaneously hypertensive rats (SHR). Methods: Ten minutes after exercise, the aorta was removed to evaluate the expression of endothelial nitric oxide synthase (eNOS), phosphorylated endothelial nitric oxide synthase (p-eNOS1177) and inducible nitric oxide synthase (iNOS) and to generate concentration-response curves to acetylcholine (ACh) and to phenylephrine (PHE). The PHE protocol was also performed with damaged endothelium and before and after NG-nitro-L-arginine methyl ester (L-NAME) and indomethacin administration. The maximal response (Emax) and the sensitivity (EC50) to these drugs were evaluated. Results: ACh-induced relaxation increased in the aortic rings of exercised (Ex) rats (Emax= -80 ± 4.6%, p < 0.05) when compared to those of controls (Ct) (Emax = -50 ± 6.8%). The Emax to PHE was decreased following exercise conditions (95 ± 7.9%, p < 0.05) when compared to control conditions (120 ± 4.2%). This response was abolished after L-NAME administration or endothelial damage. In the presence of indomethacin, the aortic rings' reactivity to PHE was decreased in both groups (EC50= Ex -5.9 ± 0.14 vs. Ct -6.6 ± 0.33 log µM, p < 0.05 / Emax = Ex 9.5 ± 2.9 vs. Ct 17 ± 6.2%, p < 0.05). Exercise did not alter the expression of eNOS and iNOS, but increased the level of p-eNOS. Conclusion: A single resistance exercise session improves endothelial function in hypertensive rats. This response seems to be mediated by increased NO production through eNOS activation.

Resumo Fundamento: O exercício físico é uma importante ferramenta para o aprimoramento da função endotelial. Objetivo: Avaliar os efeitos do exercício dinâmico resistido agudo na função endotelial de ratos espontaneamente hipertensos (SHR). Métodos: Após 10 minutos de exercício, a aorta foi removida para avaliação da expressão de óxido nítrico sintase endotelial (eNOS), óxido nítrico sintase endotelial fosforilada (p-eNOS1177) e óxido nítrico sintase endotelial induzível (iNOS), e para a construção de curvas concentração-resposta de acetilcolina (ACT) e fenilefrina (FEN). O protocolo FEN foi também realizado com lesão endotelial e antes e depois da administração de N-nitro-L-arginina metil éster (L-NAME) e indometacina. A resposta máxima (Emax) e a sensibilidade (EC50) a esses fármacos foram avaliadas. Resultados: Houve aumento do relaxamento induzido por ACT nos anéis aórticos dos ratos exercitados (Ex) (Emax = -80 ± 4,6%; p < 0,05) quando comparado àquele dos controles (Ct) (Emax = -50 ± 6,8%). A Emax à FEN diminuiu após exercício (95 ± 7,9%; p < 0,05) quando comparada àquela dos controles (120 ± 4,2%). Tal resposta foi abolida após administração de L-NAME ou lesão endotelial. Na presença de indometacina, a reatividade dos anéis aórticos à FEN diminuiu nos dois grupos (EC50= Ex -5,9 ± 0,14 vs. Ct -6,6 ± 0,33 log µM; p < 0,05/ Emax = Ex 9,5 ± 2,9 vs. Ct 17 ± 6,2%; p < 0,05). O exercício não alterou a expressão de eNOS e de iNOS, mas aumentou o nível de p-eNOS. Conclusão: Uma única sessão de exercício resistido melhora a função endotelial em ratos hipertensos. Essa resposta parece ser mediada por elevação da produção de NO através de ativação de eNOS.
Descritores: Aorta Torácica/fisiopatologia
Aorta Torácica/metabolismo
Condicionamento Físico Animal/fisiologia
Endotélio Vascular/fisiopatologia
Endotélio Vascular/metabolismo
-Aorta Torácica/química
Fenilefrina
Fosforilação/fisiologia
Fatores de Tempo
Vasoconstrição/fisiologia
Endotélio Vascular/química
Acetilcolina
Prostaglandinas/metabolismo
Western Blotting
NG-Nitroarginina Metil Éster
Óxido Nítrico Sintase Tipo II/metabolismo
Óxido Nítrico Sintase Tipo III/análise
Óxido Nítrico Sintase Tipo III/metabolismo
Teste de Esforço
Hipertensão/fisiopatologia
Hipertensão/metabolismo
Óxido Nítrico/análise
Óxido Nítrico/metabolismo
Limites: Animais
Masculino
Tipo de Publ: Estudo de Avaliação
Responsável: BR1.1 - BIREME


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Soares, Cássia Baldini
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Id: lil-731297
Autor: Campos, Heloisa da Veiga; Soares, Cássia Baldini.
Título: Practices in primary health care oriented toward the harmful consumption of drugs / Las prácticas en la atención primaria de salud relacionados con el consumo nocivo de las drogas / Práticas na Atenção Básica voltadas para o consumo prejudicial de drogas
Fonte: Rev. Esc. Enferm. USP;48(spe):109-115, 08/2014.
Idioma: en.
Resumo: Objective To analyze the practices of primary care focused on the harmful consumption of drugs. Method This is a qualitative study, developed with a dialectical-critical approach. Data collection was carried out through semi-structured interviews with 10 employees of a basic health unit (UBS). Results The demands are not accepted, and if they go beyond the barriers shaped by the historical absence of health care practices for drug users and moralistic and preconceived ideologies, they are not reinterpreted as health needs; practices that meet these demands and go beyond the barriers are poor; the functionalist approach, which explains drug use as a disease and considers drug users as deviants, supports the few existing practices. Conclusion primary health care is mistakenly focused on addiction; it lacks structural elements of the production process in health and internal dynamics of the working processes that would foster the development of collective practices. .

Objetivo El estudio tiene como objetivo analizar las prácticas de atención primaria dirigidos a lo consumo prejudicial de drogas. Método Se trata de un estudio cualitativo, desarrollado en la perspectiva dialéctica crítica. La recolección de datos se realizó a través de entrevistas semiestructuradas con 10 empleados de una Unidad Básica de Salud. Resultados Muestran que: las demandas no son aceptadas, y si van más allá de las barreras - formadas por la ausencia histórica de la práctica de la atención de salud para los consumidores de drogas y las ideologías morales y preconcebidas -, no son reinterpretados como necesidades de salud; las prácticas que satisfagan esas demandas son pobres; detrás de estas escasas prácticas, está la perspectiva funcionalista, que considera el uso de drogas como una enfermedad y los usuarios de drogas como desviados; los trabajadores valoran la formación clínica y culpan a los usuarios por los problemas que enfrentan. Conclusión Se pode concluir que la atención primaria: es equívoca hacia el objeto de la dependencia; carece de los elementos estructurales del proceso de producción en la salud y las dinámicas internas de los procesos de trabajo que fomenten el desarrollo de las prácticas colectivas.

 .

Objetivo Analisar as práticas de atenção básica voltadas ao consumo prejudicial de drogas. Método Estudo qualitativo, desenvolvido na perspectiva dialético-crítica. A coleta de dados foi realizada através de entrevistas semiestruturadas com 10 trabalhadores de uma Unidade Básica de Saúde (UBS). Resultados As demandas não são acolhidas e, quando ultrapassam as barreiras - conformadas pela ausência histórica de práticas de atenção à saúde ao usuário de drogas e por ideologias moralistas e preconceituosas -, não são reinterpretadas como necessidades de saúde; as práticas que atendem essas demandas são precárias; a perspectiva funcionalista, que compreende o consumo de drogas como doença e considera usuários de drogas como desviantes, embasa as escassas práticas existentes. Conclusão A atenção básica encontra-se equivocamente voltada para a dependência; carece de elementos estruturais do processo de produção em saúde e da dinamicidade interna aos processos de trabalho, que favoreceriam o desenvolvimento de práticas coletivas. .
Descritores: Fatores de Crescimento Endotelial/metabolismo
Linfocinas/metabolismo
Óxido Nítrico Sintase/metabolismo
Neoplasias Gástricas/irrigação sanguínea
Neoplasias Gástricas/metabolismo
-Imuno-Histoquímica
Microcirculação/patologia
Estadiamento de Neoplasias
Neovascularização Patológica
Óxido Nítrico Sintase Tipo II
Prognóstico
Neoplasias Gástricas/patologia
Fator A de Crescimento do Endotélio Vascular
Fatores de Crescimento do Endotélio Vascular
Limites: Adulto
Idoso
Feminino
Humanos
Masculino
Pessoa de Meia-Idade
Responsável: BR1.1 - BIREME


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Id: biblio-1039115
Autor: Bristot, Giovana; Ascoli, Bruna M; Scotton, Ellen; Géa, Luiza P; Pfaffenseller, Bianca; Kauer-Sant'Anna, Márcia.
Título: Effects of lithium on inflammatory and neurotrophic factors after an immune challenge in a lisdexamfetamine animal model of mania
Fonte: Braz. J. Psychiatry (São Paulo, 1999, Impr.);41(5):419-427, Sept.-Oct. 2019. tab, graf.
Idioma: en.
Resumo: Objective: To evaluate whether an animal model of mania induced by lisdexamfetamine dimesylate (LDX) has an inflammatory profile and whether immune activation by lipopolysaccharides (LPS) has a cumulative effect on subsequent stimuli in this model. We also evaluated the action of lithium (Li) on inflammatory and neurotrophic factors. Methods: Adult male Wistar rats were subjected to an animal model of mania. After the open-field test, they were given LPS to induce systemic immune activation. Subsequently, the animals' blood was collected, and their serum levels of brain-derived neurotrophic factor and inflammatory markers (tumor necrosis factor [TNF]-α, interleukin [IL]-6, IL-1β, IL-10, and inducible nitric oxide synthase [iNOS]) were measured. Results: LDX induced hyperactivity in the animals, but no inflammatory marker levels increased except brain-derived neurotrophic factor (BDNF). Li had no effect on serum BDNF levels but prevented iNOS levels from increasing in animals subjected to immune activation. Conclusion: Although Li prevented an LPS-induced increase in serum iNOS levels, its potential anti-inflammatory effects in this animal model of mania were conflicting.
Descritores: Transtorno Bipolar/imunologia
Modelos Animais de Doenças
Dimesilato de Lisdexanfetamina
Lítio/farmacologia
Anti-Inflamatórios/farmacologia
Fatores de Crescimento Neural/efeitos dos fármacos
-Fatores de Tempo
Transtorno Bipolar/fisiopatologia
Transtorno Bipolar/induzido quimicamente
Ensaio de Imunoadsorção Enzimática
Lipopolissacarídeos/farmacologia
Reprodutibilidade dos Testes
Citocinas/sangue
Resultado do Tratamento
Ratos Wistar
Fator Neurotrófico Derivado do Encéfalo/sangue
Óxido Nítrico Sintase Tipo II/sangue
Locomoção/efeitos dos fármacos
Limites: Animais
Masculino
Tipo de Publ: Estudo de Validação
Responsável: BR1.1 - BIREME


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Id: biblio-1001091
Autor: Büyük, Başak; Demirci, Tuba; Adalı, Yasemen; Eroğlu, Hüseyin Avni.
Título: A new organ preservation solution for static cold storage of the liver. Amniotic fluid
Fonte: Acta cir. bras;34(4):e201900402, 2019. tab, graf.
Idioma: en.
Projeto: The Scientific and Technological Research Council of Turkey.
Resumo: Abstract Purpose: To evaluate the effect of amniotic fluid in liver preservation in organ transplantation, and compare it with standard preservation solutions. Methods: The groups consisted of Group 1: Ringer Lactate (RL) group, Group 2: HTK group, Group 3: UW group, Group 4: AF group. The livers of rats from Group 1, 2, 3, and 4 were perfused and placed into falcon tubes containing RL, HTK, UW, and AF solutions at +4‎°C, respectively. The tubes were stored for 12 hours in the refrigerator at +4°C. Tissue samples were taken at the 6th and 12th hours for histopathological examinations of the perfused livers, and storage solutions for biochemical analyzes at 6th and 12th hours. Results: AF was shown to maintain organ viability by reducing the number of cells undergoing apoptosis. Histopathological changes such as sinusoidal dilatation, hydropic degeneration, and focal necrosis were found to be similar to the groups in which the standard organ preservation solutions were used. Additionally, the results of INOS, IL-10, and TNF-α,which were evaluated immunohistochemically, have been shown to be similar to the UW and HTK groups. Conclusions: AF provided conservation similar to UW and HTK in the 12-hour liver SCS process. The fact that apoptosis values are comparable to standard preservation solutions supports the success of AF in the cold storage of the liver.
Descritores: Criopreservação/métodos
Soluções para Preservação de Órgãos/farmacologia
Líquido Amniótico
Fígado/irrigação sanguínea
Fígado/patologia
-Preservação de Órgãos/métodos
Cloreto de Potássio/farmacologia
Procaína/farmacologia
Valores de Referência
Fatores de Tempo
Sobrevivência de Tecidos
Imuno-Histoquímica
Traumatismo por Reperfusão/prevenção & controle
Distribuição Aleatória
Reprodutibilidade dos Testes
Fator de Necrose Tumoral alfa/análise
Interleucina-10/análise
Ratos Wistar
Marcação In Situ das Extremidades Cortadas
Óxido Nítrico Sintase Tipo II/análise
Solução de Ringer/farmacologia
Glucose/farmacologia
Manitol/farmacologia
Limites: Animais
Masculino
Tipo de Publ: Estudo Comparativo
Estudo de Avaliação
Responsável: BR1.1 - BIREME


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Texto completo
Id: lil-795992
Autor: Jerônimo, Márcio Sousa; Barros, Adria do Prado; MoritaI, Vinícius Elord Zen; Alves, Erika Oliveira; Souza, Nathalia Lobão Barroso de; Almeida, Rosane Mansan de; Nóbrega, Yanna Karla Medeiros; Cavalcanti Neto, Florêncio Figueiredo; Amorin, Rivadávio; Borin, Maria de Fátima; Bocca, Anamélia Lorenzetti.
Título: Oral or topical administration of L-arginine changes the expression of TGF and iNOS and results in early wounds healing
Fonte: Acta cir. bras;31(9):586-596, Sept. 2016. tab, graf.
Idioma: en.
Projeto: CAPES, CNPq, FINATEC, DPP/UnB, and FAPDF.
Resumo: ABSTRACT PURPOSE: To evaluate the contribution of L-arginine oral or topical rout of administration in the surgical wound healing process. METHODS: L-arginine was orally or topically administrated to mice after a laparotomy model procedure. The wounds were analyzed to evaluate the granulation tissue by HE analysis, collagen deposition, iNOS and cytokines production by immunochemisyry on wound progress. Mice used in this model were healthy, immunosupressed or diabetic and all of them were treated with different concentration of L-arginine and rout of administration. RESULTS: Suggested that groups treated with L-arginine orally or topically improved wound repair when compared with non-treatad mice. L- arginine treatment stimulated TGF-β and restricted NO production leading to a mild Th1 response and collagen deposition in injured area, when it was orally administrated. Topical administration decreased IL-8 and CCR1 expression by wound cells but did not interfere with TNF-α and IL-10 production, ratifying the decrease of inflammatory response, the oral administration however, presented a higher iNOS and TGF-β expression then. L-arginine treatment also improved the improved the wound healing in immunosupressed or diabetic mice. CONCLUSION: L-arginine administrated orally or topically can be considered an important factor in the recuperation of tissues.
Descritores: Arginina/administração & dosagem
Cicatrização/efeitos dos fármacos
Citocinas/metabolismo
Fator de Crescimento Transformador beta/biossíntese
Óxido Nítrico Sintase Tipo II/biossíntese
Ferida Cirúrgica/tratamento farmacológico
-Arginina/metabolismo
Ferimentos e Lesões/patologia
Administração Oral
Administração Tópica
Colágeno/biossíntese
Hospedeiro Imunocomprometido
Diabetes Mellitus Experimental/metabolismo
Modelos Animais de Doenças
Inflamação/metabolismo
Óxido Nítrico/biossíntese
Limites: Animais
Masculino
Camundongos
Responsável: BR1.1 - BIREME


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Texto completo
Id: biblio-837708
Autor: Yagmurdur, Hatice; Binnetoglu, Kenan; Astarci, Hesna Muzeyyen; Yagmurdur, Mahmut Can.
Título: Propofol attenuates cytokine-mediated upregulation of expression of inducible nitric oxide synthase and apoptosis during regeneration post-partial hepatectomy
Fonte: Acta cir. bras;32(5):396-406, May 2017. tab, graf.
Idioma: en.
Resumo: Abstract Purpose: To determine the effects of propofol and ketamine anesthesia on liver regeneration in rats after partial hepatectomy (PHT). Methods: Male Wistar albino rats were assigned randomly to four groups of 10. Anesthesia was induced and maintained with propofol in groups 1 and 2, and with ketamine in groups 3 and 4. PHT was undertaken in groups 1 and 3. Rats in groups 2 and 4 (control groups) underwent an identical surgical procedure, but without PHT. At postoperative day-5, rats were killed. Regenerated liver was removed, weighed, and evaluated (by immunohistochemical means) for expression of inducible nitric oxide synthase (iNOS), endothelial NOS (eNOS), apoptosis protease-activating factor (APAF)-1, and proliferating cell nuclear antigen (PCNA). Also, blood samples were collected for measurement of levels of tumor necrosis factor (TNF)-α and interleukin (IL)-6. Results: Between groups 2 and 4, there were no differences in tissue levels of iNOS, eNOS, and APAF-1 or plasma levels of TNF-α and IL-6. eNOS expression was similar in group 1 and group 3. Expression of iNOS and APAF-1 was mild-to-moderate in group 1, but significantly higher in group 3. Groups 1 and 3 showed an increase in PCNA expression, but expression in both groups was comparable. Plasma levels of TNF-α and IL-6 increased to a lesser degree in group 1 than in group 3. Conclusion: Propofol, as an anesthetic agent, may attenuate cytokine-mediated upregulation of iNOS expression and apoptosis in an animal model of liver regeneration after partial hepatectomy.
Descritores: Propofol/farmacologia
Apoptose
Anestésicos Intravenosos/farmacologia
Óxido Nítrico Sintase Tipo II/metabolismo
Ketamina/farmacologia
Regeneração Hepática/efeitos dos fármacos
-Distribuição Aleatória
Propofol/metabolismo
Regulação para Cima
Interleucina-6/metabolismo
Interleucina-6/sangue
Ratos Wistar
Antígeno Nuclear de Célula em Proliferação/metabolismo
Anestésicos Intravenosos/metabolismo
Modelos Animais
Óxido Nítrico Sintase Tipo III/metabolismo
Fator Apoptótico 1 Ativador de Proteases/metabolismo
Hepatectomia
Ketamina/metabolismo
Limites: Animais
Masculino
Responsável: BR1.1 - BIREME


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Id: biblio-837673
Autor: Cunha, Joao Paulo da; Lizarte Neto, Fermino Sanches; Novais, Paulo Cezar; Gattas, Daniela; Carvalho, Camila Albuquerque Mello de; Tirapelli, Daniela Pretti da Cunha; Molina, Carlos Augusto Fernandes; Tirapelli, Luis Fernando; Tucci Junior, Silvio.
Título: Expression profiles of eNOS, iNOS and microRNA-27b in the corpus cavernosum of rats submitted to chronic alcoholism and Diabetes mellitus
Fonte: Acta cir. bras;32(1):38-45, Jan. 2017. graf.
Idioma: en.
Resumo: Abstract Purpose: To evaluate the expression of endothelial and inducible NOS in addition to the miRNA-27b in the corpus cavernosum and peripheral blood of healthy rats, diabetic rats, alcoholic rats and rats with both pathologies. Methods: Forty eight Wistar rats were divided into four groups: control (C), alcoholic (A), diabetic (D) and alcoholic-diabetic (AD). Samples of the corpus cavernosum were prepared to study protein expressions of eNOS and iNOS by immunohistochemistry and expression of miRNA-27b in the corpus cavernosum and peripheral blood. Results: Immunohistochemistry for eNOS and iNOS showed an increase in cavernosal smooth muscle cells in the alcoholic, diabetic and alcoholic-diabetic groups when compared with the control group. Similarly, the mRNA levels for eNOS were increased in cavernosal smooth muscle (CSM) in the alcoholic, diabetic and alcoholic-diabetic groups and miRNA-27b were decreased in CSM in the alcoholic, diabetic and alcoholic-diabetic groups. Conclusion: The major new finding of our study was an impairment of relaxation of cavernosal smooth muscle in alcoholic, diabetic, and alcoholic-diabetic rats that involved a decrease in the nitric oxide pathway by endothelium-dependent mechanisms accompanied by a change in the corpus cavernosum contractile sensitivity.
Descritores: Pênis/química
MicroRNAs/análise
Diabetes Mellitus Experimental/metabolismo
Alcoolismo/metabolismo
Óxido Nítrico Sintase Tipo II/análise
Óxido Nítrico Sintase Tipo III/análise
-Pênis/fisiopatologia
Imuno-Histoquímica
Ratos Wistar
Diabetes Mellitus Experimental/complicações
Diabetes Mellitus Experimental/fisiopatologia
Alcoolismo/complicações
Alcoolismo/fisiopatologia
Limites: Animais
Masculino
Ratos
Responsável: BR1.1 - BIREME



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