Base de dados : LILACS
Pesquisa : D08.811.682.732.332 [Categoria DeCS]
Referências encontradas : 144 [refinar]
Mostrando: 1 .. 10   no formato [Detalhado]

página 1 de 15 ir para página                         

  1 / 144 LILACS  
              next record last record
seleciona
para imprimir
Fotocópia
Texto completo
Id: biblio-848203
Autor: Rovaris, Eliel; Faraco, Alexandre; Simões, Priscila Waleska; Madeira, Kristian; Prá, Samira Dal-Toé de; Michels, Monique; Vieira, Andriele; Burger, Henrique; Sonai, Beatriz; Streck, Emilio; Dal-Pizzol, Felipe.
Título: Estresse oxidativo e parâmetros inflamatórios em pacientes com doença celíaca / Oxidative stress and inflammatory parameters inpatients presenting celiac disease
Fonte: Braspen J;32(2):155-159, abr.-jun. 2017.
Idioma: pt.
Resumo: Objetivos: Determinar a presença de estresse oxidativo e inflamação no intestino de pacientes com doença celíaca. Método: Foi realizado estudo transversal que incluiu pacientes submetidos à endoscopia gastrointestinal. A população do estudo consistiu em 24 casos e 26 controles. Foram medidos os níveis duodenais de proteínas carboniladas, espécies reativas ao ácido tiobarbitúrico, bem como catalase (CAT), superóxido dismutase (SOD). Também foram determinados os níveis intestinais de interleucina (IL) 6, 10 e 8. A classificação de Marsh foi registrada e utilizada como parâmetro de gravidade da doença. Resultados: Tanto a IL-6 como a IL-10, mas não a IL8, aumentaram nos pacientes com doença celíaca quando comparados com indivíduos saudáveis. Os parâmetros de dano oxidativo foram aumentados,enquanto que as defesas antioxidantes foram reduzidas em nossa amostra. Os níveis de IL6 ea atividade do SOD foram relacionados com a pontuação de Marsh. Conclusões: Diferentes marcadores de inflamação e estresse oxidativo estão alterados no intestino de pacientes com doença celíaca, e alguns deles estão relacionados à gravidade da doença.(AU)

Objectives: Determine the presence of oxidative stress and inflammation in the gut of patients with celiac disease. Methods: Transversal study that included patients undergoing upper gastrointestinal endoscopy was performed. The study population consisted 24 cases and 26 controls. The duodenal levels of protein carbonyls, thiobarbituric acid reactive species, as well as catalase, superoxide dismutase (SOD) activities were measured. Gut levels of interleukin (IL) 6, 10 and 8 were also determined.The Marsh classification was recorded and used as a parameter of disease severity. Results: Both IL-6 and IL-10, but not IL8, were increased in celiac disease patients when compared to healthy individuals. Oxidative damage parameters were increased while antioxidant defenses were decreased in our sample. Both IL6 levels and SOD activity were related to Marsh score. Conclusions: Different markers of inflammation and oxidative stress are altered in the gut of celiac disease patients, and some of them are related to disease severity.(AU)
Descritores: Doenças Inflamatórias Intestinais
Doença Celíaca/patologia
Estresse Oxidativo
-Superóxido Dismutase/análise
Catalase/análise
Estudos Transversais/instrumentação
Endoscopia Gastrointestinal/instrumentação
Interleucinas/análise
Substâncias Reativas com Ácido Tiobarbitúrico/análise
Carbonilação Proteica
Limites: Seres Humanos
Tipo de Publ: Estudo Comparativo
Responsável: BR1.1 - BIREME


  2 / 144 LILACS  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
Id: biblio-1021571
Autor: Jia, Xianbo; Lin, Xinjian; Lin, Chenqiang; Lin, Lirong; Chen, Jichen.
Título: Enhanced alkaline catalase production by Serratia marcescens FZSF01: enzyme purification, characterization, and recombinant expression
Fonte: Electron. j. biotechnol;30:110-117, nov. 2017. graf, tab, ilus.
Idioma: en.
Projeto: Agro-scientific Research in the Public Interest of China; . Fujian Provincial Public Welfare Institutes of Special Scientific Research in China 2013.
Resumo: Background: Catalase (CAT) is an important enzyme that degrades H2O2 into H2O and O2. To obtain an efficient catalase, in this study, a new strain of high catalase-producing Serratia marcescens, named FZSF01, was screened and its catalase was purified and characterized. Results: After optimization of fermentation conditions, the yield of catalase produced by this strain was as high as 51,468 U/ml. This catalase was further purified using two steps: DEAE-fast flow and Sephedex-G150. The purified catalase showed a specific activity of 197,575 U/mg with a molecular mass of 58 kDa. This catalase exhibited high activity at 20­70°C and pH 5.0­11.0. Km of the catalase was approximately 68 mM, and Vmax was 1886.8 mol/min mg. This catalase was further identified by LC­MS/MS, and the encoding gene was cloned and expressed in Escherichia coli BL21 (DE3) with a production of 17,267 ± 2037 U/ml. Conclusions: To our knowledge, these results represent one of the highest fermentation levels reported among current catalase-producing strains. This FZSF01 catalase may be suitable for several industrial applications that comprise exposure to alkaline conditions and under a wide range of temperatures.
Descritores: Serratia marcescens/enzimologia
Catalase/metabolismo
-Recombinação Genética
Serratia marcescens/genética
RNA Ribossômico 16S
Cinética
Catalase/isolamento & purificação
Catalase/genética
Cromatografia Líquida
Análise de Sequência de DNA
Eletroforese
Escherichia coli/genética
Escherichia coli/metabolismo
Fermentação
Peróxido de Hidrogênio/metabolismo
Responsável: CL1.1 - Biblioteca Central


  3 / 144 LILACS  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo SciELO Brasil
Texto completo
Texto completo
Id: biblio-898913
Autor: Lazarte, Sandra Stella; Mónaco, María Eugenia; Terán, Magdalena María; Haro, Ana Cecilia; Achem, Miryam Emilse Ledesma; Issé, Blanca Alicia.
Título: Foxo3 gene expression and oxidative status in beta-thalassemia minor subjects
Fonte: Rev. bras. hematol. hemoter;39(2):115-121, Apr.-June 2017. tab, graf.
Idioma: en.
Projeto: Consejo de Investigaciones de la Universidad Nacional de Tucumán.
Resumo: ABSTRACT Background: Oxidative stress may aggravate symptoms of hemolytic anemias such as beta-thalassemia. FoxO3 activation results in resistance to oxidative stress in fibroblasts and neuronal cell cultures. Objective: The purpose of this research was to study FoxO3 gene expression and oxidative status in beta-thalassemia minor individuals. Methods: Sixty-three subjects (42 apparently healthy individuals and 21 with beta-thalassemia minor) were analyzed at the Universidad Nacional de Tucumán, Argentina, between September 2013 and June 2014. A complete blood count, hemoglobin electrophoresis in alkaline pH and hemoglobin A2 levels were quantified. Moreover, thiobarbituric acid reactive species, erythrocyte catalase activity and iron status were evaluated. Beta-thalassemia mutations were determined by real-time polymerase chain reaction. FoxO3 gene expression was investigated by real-time reverse transcription-polymerase chain reaction using mononuclear cells from peripheral blood. Results: Subjects were grouped as children (≤12 years), and adult women and men. The analysis of erythrocyte catalase activity/hemoglobin ratio revealed a significant difference (p-value <0.05) between healthy and beta-thalassemia minor adults, but no significant difference was observed in the thiobarbituric acid reactive species levels and FoxO3 gene expression (p-value >0.05). Thiobarbituric acid reactive species and the erythrocyte catalase activity/hemoglobin ratio were not significantly different on comparing the type of beta-thalassemia mutation (β0 or β+) present in carriers. Conclusions: The lack of systemic oxidative imbalance demonstrated by thiobarbituric acid reactive species is correlated to the observation of normal FoxO3 gene expression in mononuclear cells of peripheral blood. However, an imbalanced antioxidant state was shown by the erythrocyte catalase activity/hemoglobin ratio in beta-thalassemia minor carriers. It would be necessary to study FoxO3 gene expression in reticulocytes to elucidate the role of FoxO3 in this pathology.
Descritores: Catalase
Substâncias Reativas com Ácido Tiobarbitúrico
Talassemia beta/terapia
Estresse Oxidativo
Eritrócitos
Proteína Forkhead Box O3
Limites: Seres Humanos
Masculino
Feminino
Responsável: BR408.1 - Biblioteca da Faculdade de Medicina - BFM


  4 / 144 LILACS  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
Id: lil-429028
Autor: Adamcryk Wieler, Lorena; Trigoso Agudo, Christian; Vasquez Michel, Aneth; Duran Arias, Loretta.
Título: Determinacion de la Homoresistencia y Heteroresistencia de Staphylococcus Aereus Meticilinoresistente aislado de muestras de pacientes internados en los hospitale Boliviano Holandes, Hospital Obrero y Hospital de Clinicas / Determination of the Homoresistence and Heteroresistence of methicillin resistant staphylococcus aureus isolated from patient admitted to the hospital Boliviano Holandes, Hospital Obrero and Hospital de Clinicas
Fonte: Cuad. Hosp. Clín = Cuad. - Hosp. clín;50(2):7-11, 2005. tab.
Idioma: es.
Resumo: PREGUNTA DE INVESTIGACIÓN ¿Las cepas de Staphylococcus aureus meticilino resistentes circulantes en nuestro medio, serán homoresistentes o heteroresistentes? OBJETIVOS: determinar los fenómenos de homoresistencia y heteroresistencia a meticilina en aislamientos de Staphylococcus aureus de muestras de pacientes internados en los Hospitales Boliviano Holandes, Hospital Obrero y Hospital de Clínicas de la Ciudad de La Paz Determinar el porcentaje de cepas de Staphylococcus aureus resistentes a la meticilina en los aislamientos obtenidos DISEÑO Corte transversal LUGAR Instituto Nacional de Laboratorios en Salud (INLASA) MUESTRAS Las cepas fueron aisladas de muestras de exudados purulentos de abscesos, heridas y quemaduras provenientes de pacientes internados en los Hospitales Boliviano Holandes, Hospital Obrero y Hospital de Clínicas. MÉTODOS: se realizó la identificación de género y especie aureus mediante pruebas de catalasa; coagulasa y manitol respectivamente. La determinación de sensibilidad y /o resistencia se las hizo mediante difusión en disco (Bauer-Kirby) con discos de Oxacilina de 1 µg La Concentración Inhibitoria Mínima (CIM) se la determinó por microdilución en caldo con Oxacilina p.a en polvo RESULTADOS: de 100 aislamientos de Staphylococcus aureus, el 16% son resistentes a la meticilina En ninguna de las cepas resistentes se pudo evidenciar homoresistencia a la meticilina, por tanto el 100% de las cepas son heteroresistentes. CONCLUSIONES: se determina que en nuestro medio la meticilina mantiene una buena actividad antimicrobiana frente a Staphylococcus aureus. No existe el fenómeno de homoresistencia en las cepas aisladas en este estudio.

RESEARCH QUESTION Are the circulating methicillin resistant staphylococcus aureus strains in our patients homoresistant or heteroresistant? OBJECTIVES: To assess the homoresistance and heteroresistance to methicillin of Staphylococcus aureus isolated from purulent exudate samples obtained from patients admitted to the Hospital Boliviano Holandes, Hospital Obrero and Hospital de Clinicas in La Paz. To determine the percentage of methicillin resistant Staphylococcus aureus in these samples. Study Design Cross sectional Study Location Instituto Nacional de Laboratorios de Salud (INLASA) Samples The strains were isolated from samples of purulent discharges from abscesses, wounds, infected burns from patients admitted to the Hospital Boliviano Holandes, Hospital Obrero, and Hospital de Clinicas. METHODS: Staphylococcus aureus was identified by tests of catalase, coagulase and manitol. Sensitivity and/or resistance was determined by disc diffusion (Bauer Kirby) with discs of Oxacilina of 1 µg The Minimal Inhibitory Concentration (CIM) was determined by microdilution. RESULTS: of 100 Staphylococcus aureus isolations, 16% were methicillin resistant. None of these strains was shown to be homoresistant. Therefore, we assume that 100% of the strains were heteroresistant. CONCLUSIONS: methicillin maintains a good antimicrobial activity against Staphylococcus aureus. The isolations in our study did not show homoresistance.
Descritores: Staphylococcus aureus
Meticilina
-Oxacilina
Catalase
Limites: Seres Humanos
Masculino
Feminino
Tipo de Publ: Relatos de Casos
Responsável: BO6.1 - Biblioteca


  5 / 144 LILACS  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo SciELO Brasil
Texto completo
Texto completo
Id: biblio-1000033
Autor: Pittner, Elaine; Marek, Janaina; Bortuli, Douglas; Santos, Leandro Alvarenga; Knob, Adriana; Faria, Cacilda Marcia Duarte Rios.
Título: Defense responses of wheat plants (Triticum aestivum L) against brown spot as a result of possible elicitors application / Resposta de defesa das plantas de trigo (Triticum aestivum L) contra a mancha marrom diante da aplicação de possíveis elicitores
Fonte: Arq. Inst. Biol;86:e0312017, 2019. tab, graf.
Idioma: en.
Resumo: The objective of this research was to evaluate the response of wheat plants to the application of possible elicitor compounds against Bipolaris sorokiniana pathogen. This response was measured through the quantification of antioxidant enzymes, malondialdehyde and flavonoids, evaluation of the severity of brown spot disease and productivity in wheat, greenhouse and field crops. The treatments consisted of suspensions of endophytic fungi Aspergillus japonicus and Trichoderma tomentosum, salicylic acid, acibenzolar-S-methyl and fungicide. In the field trials, in 2015 and 2016, the development of the disease was lower and productivity was higher in all treatments, with emphasis on the fungicide. However, endophytic fungi suspensions demonstrated potential as growth promoters, disease severity reducers and protective antioxidant response activators, as they promoted significant increase in superoxide dismutase, catalase, glutathione and flavonoid enzymes.(AU)

O objetivo desta pesquisa foi avaliar a resposta de plantas de trigo diante da aplicação de possíveis compostos elicitores perante o patógeno Bipolaris sorokiniana. Tal resposta foi examinada por meio da quantificação de enzimas antioxidantes, malondialdeído e flavonoides, da análise da severidade da doença mancha marrom e da produtividade na cultura do trigo, em casa de vegetação e em campo. Os tratamentos consistiram em suspensões de fungos endofíticos Aspergillus japonicus e Trichoderma tomentosum, ácido salicílico, acibenzolar-S-metil e fungicida. Nos ensaios em campo, em 2015 e 2016, o desenvolvimento da doença foi menor e a produtividade foi superior em todos os tratamentos, com destaque para o fungicida. No entanto, as suspensões de fungos endofíticos demonstraram potencial como promotores de crescimento, redutores da severidade da doença e ativadores de resposta antioxidante protetora, pois promoveram o aumento significativo das enzimas superóxido dismutase e catalase, das glutationas e flavonoides.(AU)
Descritores: Triticum
Noxas
-Aspergillus
Trichoderma
Flavonoides
Catalase
Glutationa
Responsável: BR1942.1 - NID - Biblioteca - Núcleo de Informação e Documentação


  6 / 144 LILACS  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
Id: lil-632991
Autor: Araujo, Carmen Rosa; Posleman, Sara Emilia; de la Cruz Rodríguez, Lilia Cristina.
Título: Cambios en el sistema de defensa antioxidante inducido por Ciclosporina A / Changes in the antioxidant defence system induced by Cyclosporin A
Fonte: Acta bioquím. clín. latinoam;41(1):35-45, ene.-mar. 2007. ilus, graf, tab.
Idioma: es.
Resumo: La Ciclosporina A (CyA) es un inmunosupresor que presenta efectos adversos como la hepatotoxicidad. Se estudió el efecto de CyA sobre el sistema de defensa antioxidante (SDA), su relación con la lipoperoxidación y la función hepática. Ratas machos wistar de 200-260 g de peso fueron tratadas durante 7 días (agudo) y 120 días (crónico) con dosis orales de CyA de 5 y 20 mg/kg/ día. Se estudió el SDA midiendo el contenido hepático total de glutatión (GSH), glutatión peroxidasa (GPx) y catalasa (CAT); el perfil de funcionamiento hepático (PFH) se realizó determinando aspartato aminotransferasa (AST), alanín aminotransferasa (ALT) y bilirrubina total (Bt) y para la lipoperoxidación se midieron las sustancias reactivas al ácido tiobarbitúrico (SRAT). Los resultados fueron confirmados con estudios histológicos. El tratamiento agudo con 20 mg/kg/día de CyA mostró aumento significativo de SRAT (30,51±1,97 nmol/g), pérdida de GSH (2,47±0,06 µmol/g), incremento significativo de GPx (663,25±1,88 mU/mg) y CAT (290,65±3,31 mU/mg). El tratamiento crónico con 5 mg/kg/día de CyA mostró disminución tiempo-dependiente del SDA con disminución de GSH (3,19±0,05 µmol/g), GPx (569,6±2,67 mU/mg) y CAT (223,3±2,78 mU/mg), sin cambios en SRAT. Los resultados del tratamiento crónico y agudo con 20 mg/kg/día de CyA son coincidentes y sólo en esta dosis se observaron alteraciones de la histo-arquitectura del parénquima hepático. Se concluye que dosis de 20 mg/kg/día de CyA en tratamiento agudo y crónico provocan lipoperoxidación con compromiso del SDA y alteración del hepatocito; dosis de 5 mg/kg/día de CyA en tratamiento crónico producen deterioro reversible del SDA sin lipoperoxidación. La inmunosupresión aplicada en clínica con dosis de 3 a 8 mg/kg/día produciría disminución del SDA sin cambios en la histo-arquitectura del parénquima hepático.

Cyclosporin A (CyA), an immunosuppressive agent, exerts adverse effects such as hepatotoxicity. The effect of CyA on the Antioxidant Defence System(ADS), its relation to lipoperoxidation, and liver function were studied. Assays were performed on male wistar rats weighing 200-260 g during acute (7 days) and chronic (120 days) treatment with oral doses of CyA of 5 and 20 mg/kg/day. ADS was studied in rat liver homogenate by measuring the liver content of total glutathion (GSH), glutathion peroxidase(GPx) and catalase (CAT); the Liver Profile Test (LPT) was measured by determining aspartate amino transferase (AST), alanin amino transferase (ALT) and total bilirubin (TB), and lipoperoxidation by determining thiobarbituric acid reactive substances (TRAS). The results were confirmed by histological studies. In the acute treatment, 20 mg/kg/day with CyA, a significant increase in TRAS (30.51±1.97 nmol/g), a loss of GSH (2.47±0.06 µmol/g) and a significant increase in GPx (663.25±1.88 mU/mg) and CAT (290.65±3.31 mU/mg) were observed. In the chronic treatment, 5 mg/kg/day with CyA, a time-dependent decrease in the ADS with a diminution in GSH (3.19±0.05 µmol/g), GPx (569.6±2.67 mU/mg) and CAT (223.3±2.78 mU/mg) were observed, with no changes in TRAS. The results for the chronic and acute treatment with 20 mg/kg/day of CyA are coincident, only this dose causing alterations in liver parenchyma histoarchitecture. CyA doses of 20 mg/kg/day during acute and chronic treatment cause lipoperoxidation with ADS involvement and hepatocyte alteration. CyA doses of 5 mg/kg/day during chronic treatment cause deterioration in the ADS with no lipoperoxidation, hepatotoxicity being reversible. Immunosuppression in human patients with 3 to 8 mg/kg/day doses, would cause a decrease in the ADS with no structural or functional changes in the hepatocyte.
Descritores: Catalase
Ciclosporina
Glutationa
Glutationa Peroxidase
-Bilirrubina
Bioquímica
Peróxidos Lipídicos
Antioxidantes
Limites: Animais
Camundongos
Tipo de Publ: Ensaio Clínico
Responsável: AR144.1 - CIBCHACO - Centro de Información Biomedica del Chaco


  7 / 144 LILACS  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
Texto completo
Id: biblio-981581
Autor: Nicola Salas, Eva; Morey-León, Gabriel; Villacís Alvarado, Ninoska; Sánchez Chóez, Javier.
Título: Análisis genético de la resistencia a isoniacida en cepas de Mycobacterium tuberculosis / Genetic analysis of isoniazid resistance in strains of Mycobacterium tuberculosis
Fonte: INSPILIP;2(2):1-16, jul.-dic. 2018.
Idioma: es.
Resumo: Objetivo: Analizar genéticamente la resistencia a Isoniacida en cepas de Mycobacterium tuberculosis mediante PCR-RFLP de la región S315T del gen katG. Materiales y métodos: El estudio se realizó a partir de cultivos positivos de Mycobacterium tuberculosis receptados en el Centro de Referencia Nacional de Micobacterias, durante el período 2013 ­ 2014. El ADN extraído fue cuantificado y evaluada su pureza, por espectrofotometría. Para determinar el polimorfismo en la región 315 del gen katG a partir de un producto de amplificación de 630 pb se realizó digestiones con las enzimas de restricción MspI y SatI. Resultados: Del total de 498 cepas analizadas, 215 cepas presentaron características fenotípicas de resistencia a isoniacida (32,6 % monorresistencia, 19,5 % MDR y 47,9 % polirresistencia), 283 cepas eran sensibles. 251 cepas correspondieron a pacientes vírgenes al tratamiento (VT); 174 fueron pacientes antes tratados (AT) y 73 fueron pacientes se encontraban con tratamiento (CT). La mayoría de los casos provenía de la provincia del Guayas (77,2 %). La PCR-RFLP-SatI presentó alto porcentaje de sensibilidad (98,6 %) y especificidad (98,2 %), mientras que con la enzima MspI el porcentaje de sensibilidad fue 88,8 % y 7,4 % de especificidad. Conclusión: La PCR-RFLP-SatI demostró ser específica y económica para la detección de resistencia a isoniacida, proporcionando resultados de forma rápida, la aplicación de esta técnica como apoyo para el diagnóstico permitiría al paciente acceder a un tratamiento más oportuno.

Objective: Genetically analyze the Isoniazid resistance in Mycobacterium tuberculosis cultures by PCR-RFLP of the S315T region of the atG. Materials and methods: The study was carried out in positive cultures of Mycobacterium tuberculosis, received at the National Reference Center of Mycobacteria, during the period 2013-2014. The DNA extracted was quantified and its purity was evaluated by spectrophotometry. To determine the polymorphism in the 315 region of the at G gene, digestions were made with the restriction enzymes MspI and SatI from a 630 bp amplification product. Results: Of 498 culture strains analyzed, 215 strains showed phenotypic characteristics of resistance to Isoniazid (32,6 % monoresistance, 19,5 % MDR and 47,9 % polyresistance) and 283 strains were sensitive. 251 strains corresponded to virgin patients to treatment (VT); 174 were patients before treated (AT) and 73 were patients treated (CT). The majority of cases came from the province of Guayas (77,2 %). The PCR-RFLP- SatI presented a high percentage of sensitivity (98,6 %) and specificity (98,2 %), while with the MspI enzyme the sensitivity percentage was 88,8 % and 7,4 % specificity. Conclusion: The PCR-RFLP SatI proved to be specific and economical for the detection of resistance to isoniazid, providing results quickly, the application of this technique as a support for the diagnosis would allow the patient to access a more timely treatment.
Descritores: Tuberculose
Catalase
Digestão
Isoniazida
-Equador
Limites: Seres Humanos
Tipo de Publ: Artigo Clássico
Responsável: EC7.1 - Biblioteca


  8 / 144 LILACS  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo SciELO Brasil
Texto completo
Id: biblio-888997
Autor: Ribeiro-Samora, GA; Rabelo, LA; Ferreira, ACC; Favero, M; Guedes, GS; Pereira, LSM; Parreira, VF; Britto, RR.
Título: Inflammation and oxidative stress in heart failure: effects of exercise intensity and duration
Fonte: Braz. j. med. biol. res = Rev. bras. pesqui. méd. biol;50(9):e6393, 2017. tab, graf.
Idioma: en.
Projeto: CNPq; . FAPEMIG.
Resumo: Although acute exercise is apparently pro-inflammatory and increases oxidative stress, it can promote the necessary stress stimulus to train chronic adaptations in patients with chronic heart failure (CHF). This study aimed to compare the effects of exercise intensity and duration on the inflammatory markers soluble tumor necrosis factor receptor (sTNFR1) and interleukin-6 (IL-6), and on oxidative stress [malondialdehyde (MDA) and antioxidant enzymes: catalase (CAT) and superoxide dismutase (SOD)] in individuals with CHF. Eighteen patients performed three exercise sessions: 30 min of moderate-intensity (M30) exercise, 30 min of low-intensity (L30) exercise, and 45 min of low-intensity (L45) exercise. Blood analysis was performed before exercise (baseline), immediately after each session (after), and 1 h after the end of each session (1h after). Thirty min of M30 exercise promoted a larger stressor stimulus, both pro-inflammatory and pro-oxidative, than that promoted by exercises L30 and L45. This was evidenced by increased sTNFR1 and MDA levels after exercise M30. In response to this stressor stimulus, 1 h after exercise, there was an increase in IL-6 and CAT levels, and a return of sTNFR1 to baseline levels. These findings suggest that compared with the duration of exercise, the exercise intensity was an important factor of physiologic adjustments.
Descritores: Biomarcadores/sangue
Teste de Esforço
Insuficiência Cardíaca/imunologia
Inflamação/imunologia
Estresse Oxidativo/fisiologia
-Catalase/sangue
Doença Crônica
Insuficiência Cardíaca/sangue
Insuficiência Cardíaca/fisiopatologia
Inflamação/sangue
Inflamação/fisiopatologia
Interleucina-6/sangue
Malondialdeído/sangue
Receptores do Fator de Necrose Tumoral/sangue
Superóxido Dismutase/sangue
Limites: Seres Humanos
Masculino
Feminino
Adulto
Meia-Idade
Tipo de Publ: Estudo Comparativo
Responsável: BR1.1 - BIREME


  9 / 144 LILACS  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
Texto completo
Id: biblio-832102
Autor: Hauck, Melina; Signori, Luis Ulisses; Teixeira, Michelle Carneiro; Veloso, Carolina Fantinel; Bauermann, Liliane de Freitas; Silva, Antônio Marcos Vargas da.
Título: Trans-resveratrol reduces cardiac oxidative stress in rats exposed to cigarette smoke / O trans-resveratrol reduz o estresse oxidativo cardíaco de ratos expostos à fumaça de cigarro
Fonte: Acta sci., Health sci;37(2):133-139, jul.-dez. 2015. tab, ilus.
Idioma: en.
Resumo: Differences between reactive oxygen species and antioxidant defense system unbalance the redox status. The exposure to cigarette smoke can increase this imbalance. Trans-resveratrol is a polyphenol with great antioxidant action that reduces the oxidative stress. This study investigated the effect of the trans-resveratrol supplementation on the cardiac oxidative stress in rats exposed to cigarette smoke. Male Wistar rats were randomized into four groups: Control Group (CG), Exposure to Smoke Group (ESG), Antioxidant Group (AG) and Exposure to Smoke plus Antioxidant Group (ESAG). Animals were exposed to cigarette smoke and supplemented with trans-resveratrol (6.0 mg kg-1) for two months. The lipid peroxidation (TBARS) and the enzymatic activity of catalase (CAT) were measured in the cardiac muscle. The ESG presented the highest lipid peroxidation level compared with CG (p < 0.001), AG (p < 0.001) and ESAG (p < 0.006). The CAT activity was higher in the AG (p < 0.001) and ESAG (p < 0.001) compared with CG. The ESG presented lower CAT activity compared with the ESAG (p < 0.001). The supplementation of Trans-resveratrol attenuated the cardiac oxidative stress and increased the activity of catalase. Our findings evidenced the cardioprotective effect of trans-resveratrol in rats exposed to cigarette smoke.

Diferenças entre espécies reativas de oxigênio e sistema de defesa antioxidante desequilibram o estado redox. Exposição à fumaça de cigarro pode aumentar esse desequilíbrio. Trans-resveratrol é um polifenol com ação antioxidante que reduz o estresse oxidativo. O objetivo do presente estudo foi investigar os efeitos da suplementação com trans-resveratrol no estresse oxidativo cardíaco de ratos expostos à fumaça de cigarro. Randomização de 32 ratos Wistar machos em quatro grupos: Controle (CG), Exposição à Fumaça (ESG), Antioxidante (AG) e Exposição à Fumaça+Antioxidante (ESAG). Animais foram expostos à fumaça de cigarro e suplementados trans-resveratrol (6,0 mg kg-1) durante dois meses. Lipoperoxidação (TBARS) e atividade enzimática da catalase (CAT) foram mensuradas no músculo cardíaco. ESG apresentou maiores níveis de lipoperoxidação quando comparado ao CG (p < 0,001), AG (p < 0,001) e ao ESAG (p < 0,006). Atividade da CAT foi maior no AG (p < 0,001) e no ESAG (p < 0,001) quando comparados ao CG. ESG apresentou a menor atividade da CAT quando comparado ao ESAG (p < 0,001). A suplementação com trans-resveratrol atenuou o estresse oxidativo cardíaco e aumentou a atividade enzimática de defesa catalase. Esses resultados sugerem evidências de efeitos cardioprotetores do trans-resveratrol em ratos expostos à fumaça de cigarro.
Descritores: Catalase
Miocárdio
Polifenóis
Espécies Reativas de Oxigênio
Fumar
Limites: Ratos
Responsável: BR513.2 - Editora da Universidade Estadual de Maringá


  10 / 144 LILACS  
              first record previous record
seleciona
para imprimir
Fotocópia
Texto completo
Id: biblio-831329
Autor: Valenzuela B., Rodrigo; Hernández-Rodas, María Catalina; Barrera R., Cynthia; Pérez B., Francisco; Ruz O., Manuel.
Título: Reducción de la esteatosis hepática, insulino resistencia y pérdida de la defensa antioxidante en ratones alimentados con dieta alta en grasa suplementados con AGPICL n-3 más aceite de oliva extra virgen / Reduction of hepatic steatosis, insulin resistance and loss of antioxidant defense in high fat diet fed mice suplemented with n-3 LCPUFA more extra virgin olive oil
Fonte: Rev. chil. endocrinol. diabetes;8(4):154-161, oct. 2015. tab, ilus.
Idioma: es.
Projeto: Sociedad Chilena de Endocrinología y Diabetes.
Resumo: Non-alcoholic fatty liver disease (NAFLD) is directly associated with insulin resistance and oxidative stress. In NAFLD is established a reduction in n-3 LCPUFA (EPA + DHA) levels and hepatic activity of transcription factor PPAR-alpha. EPA and DHA inhibit lipogenesis and stimulate fatty acid oxidation in the liver. Extra virgin olive oil (EVOO) has important antioxidant properties. This study evaluated the prevention of insulin resistance and prevention of depletion of hepatic antioxidant defense inC57BL/6J mice fed high-fat diet (HFD), supplemented with n-3 LCPUFA plus EVOO. HFD generated insulin resistance and hepatic steatosis, together with significant reduction in i) n-3 LCPUFA hepatic levels, ii) DNA binding activity of PPAR-alpha, iii) activity of antioxidant enzymes (catalase and superoxide dismutase), respect to control group (fed with control diet). Supplementation with n-3 LCPUFA plus EVOO prevent development insulin resistance and attenuate increased of fat in liver (p < 0.05), together with a normalization of i) DNA binding activity of PPAR-á, ii) activity of antioxidant enzymes (catalase and superoxide dismutase) and iii) reducing depletion of n-3 LCPUFA levels in liver tissue, compared to the control group (p < 0.05). Supplementation with n-3 LCPUFA plus EVOO reduced hepatic steatosis and prevent development of insulin resistance, along with preserving the antioxidant defense in liver. Projecting the use of this mixture of AGPICL n-3 plus EVOO as a potential treatment of NAFLD.
Descritores: Azeite de Oliva/uso terapêutico
/uso terapêutico
ACIDOS GRASOS OMEGA-ABATTOIRS/uso terapêutico
Dieta Hiperlipídica
Suplementos Nutricionais
Hepatopatia Gordurosa não Alcoólica/dietoterapia
-Azeite de Oliva/farmacologia
/farmacologia
ACIDOS GRASOS OMEGA-ABATTOIRS/farmacologia
Catalase
Catalase/fisiologia
Fígado
Estresse Oxidativo
RATONES CONSANGUINEOS CABDOMENABDOMINAL INJURIESBL
Superóxido Dismutase
Limites: Masculino
Animais
Camundongos
Responsável: CL1.1 - Biblioteca Central



página 1 de 15 ir para página                         
   


Refinar a pesquisa
  Base de dados : Formulário avançado   

    Pesquisar no campo  
1  
2
3
 
           



Search engine: iAH v2.6 powered by WWWISIS

BIREME/OPAS/OMS - Centro Latino-Americano e do Caribe de Informação em Ciências da Saúde