||Sreedharan, Krishnan; Philip, Rosamma; Singh, Isaac Sarojani Bright.|
||Virulence potential and antibiotic susceptibility pattern of motile aeromonads associated with freshwater ornamental fish culture systems: a possible threat to public health|
||Braz. j. microbiol;43(2):754-765, Apr.-June 2012. ilus, tab.
||Ministry of Commerce & Industry, Govt. of India. Marine Products Export Development Authority; . Govt. of India. Department of Biotechnology.
||Aeromonas spp. are ubiquitous aquatic organisms, associated with multitude of diseases in several species of animals, including fishes and humans. In the present study, water samples from two ornamental fish culture systems were analyzed for the presence of Aeromonas. Nutrient agar was used for Aeromonas isolation, and colonies (60 No) were identified through biochemical characterization. Seven clusters could be generated based on phenotypic characters, analyzed by the programme NTSYSpc, Version 2.02i, and identified as: Aeromonas caviae (33.3%), A. jandaei (38.3%) and A. veronii biovar sobria (28.3%). The strains isolated produced highly active hydrolytic enzymes, haemolytic activity and slime formation in varying proportions. The isolates were also tested for the enterotoxin genes (act, alt and ast), haemolytic toxins (hlyA and aerA), involved in type 3 secretion system (TTSS: ascV, aexT, aopP, aopO, ascF-ascG, and aopH), and glycerophospholipid-cholesterol acyltransferase (gcat). All isolates were found to be associated with at least one virulent gene. Moreover, they were resistant to frequently used antibiotics for human infections. The study demonstrates the pathogenic potential of Aeromonas, associated with ornamental fish culture systems suggesting the emerging threat to public health.|
Aeromonas/isolamento & purificação
Suscetibilidade a Doenças
Resistência Microbiana a Medicamentos
Infecções por Bactérias Gram-Negativas
Técnicas In Vitro
Reação em Cadeia da Polimerase/métodos
Microbiologia da Água
Amostras de Água
||BR32.1 - Serviço de Biblioteca e Informação Biomédica|