Base de dados : LILACS
Pesquisa : D08.811.913.050 [Categoria DeCS]
Referências encontradas : 4 [refinar]
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Id: biblio-839294
Autor: Song, L; Duan, P; Gan, Y; Li, P; Zhao, C; Xu, J; Zhang, Z; Zhou, Q.
Título: MicroRNA-340-5p modulates cisplatin resistance by targeting LPAATβ in osteosarcoma
Fonte: Braz. j. med. biol. res = Rev. bras. pesqui. méd. biol;50(5):e6359, 2017. graf.
Idioma: en.
Resumo: MicroRNAs (miRNAs) play an important role in drug resistance and modulate the efficiency of chemotherapy. A recent study indicated that miR-340 functions as a tumor suppressor in various types of cancer. However, the role of miR-340 in chemotherapy has not been reported yet. In this study, we found that miR-340 enhanced cisplatin (CDDP)-induced cell death. Induction of miR-340-5p expression decreased the IC50 of CDDP and increased the apoptosis of CDDP-resistant MG-63 and Saos-2 cells. Moreover, miR-340-5p decreased the accumulation of MRP1 and MDR1. We further explored the mechanism underlying the promoting effects of miR-340-5p on CDDP-induced cell death. We identified a potential target of miR-340 in the 3′ untranslated region of lysophosphatidic acid acyltransferase (LPAATβ) using the online program Targetscan (http://www.microrna.org). Luciferase reporter assays showed that miR-340 binds to the 3′UTR of LPAATβ. Enforced expression of miR-340-5p decreased the accumulation of LPAATβ in both MG-63 and Saos-2 cells. Silencing LPAATβ decreased the IC50 of CDDP and increased the apoptosis of CDDP-resistant MG-63 and Saos-2 cells, which is consistent with the effect of miR-340-5p on CDDP-induced cell death. Moreover, induced expression of LPAATβ compromised the effects of miR-340-5p on CDDP-induced cell death and accumulation of MRP1 and MDR1. Taken together, our data indicated that miR-340-5p enhanced the sensitivity to CDDP by targeting LPAATβ.
Descritores: Aciltransferases/fisiologia
Antineoplásicos/farmacologia
Neoplasias Ósseas/tratamento farmacológico
Cisplatino/farmacologia
Resistencia a Medicamentos Antineoplásicos/fisiologia
MicroRNAs/fisiologia
Osteossarcoma/tratamento farmacológico
-Aciltransferases/análise
Aciltransferases/efeitos dos fármacos
Apoptose/efeitos dos fármacos
Western Blotting
Neoplasias Ósseas/fisiopatologia
Linhagem Celular Tumoral
Proliferação de Células/efeitos dos fármacos
Regulação para Baixo
Resistencia a Medicamentos Antineoplásicos/efeitos dos fármacos
Luciferases
MicroRNAs/análise
MicroRNAs/efeitos dos fármacos
Osteossarcoma/fisiopatologia
Reação em Cadeia da Polimerase em Tempo Real
Limites: Humanos
Responsável: BR1.1 - BIREME


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Id: lil-644493
Autor: Sreedharan, Krishnan; Philip, Rosamma; Singh, Isaac Sarojani Bright.
Título: Virulence potential and antibiotic susceptibility pattern of motile aeromonads associated with freshwater ornamental fish culture systems: a possible threat to public health
Fonte: Braz. j. microbiol;43(2):754-765, Apr.-June 2012. ilus, tab.
Idioma: en.
Projeto: Ministry of Commerce & Industry, Govt. of India. Marine Products Export Development Authority; . Govt. of India. Department of Biotechnology.
Resumo: Aeromonas spp. are ubiquitous aquatic organisms, associated with multitude of diseases in several species of animals, including fishes and humans. In the present study, water samples from two ornamental fish culture systems were analyzed for the presence of Aeromonas. Nutrient agar was used for Aeromonas isolation, and colonies (60 No) were identified through biochemical characterization. Seven clusters could be generated based on phenotypic characters, analyzed by the programme NTSYSpc, Version 2.02i, and identified as: Aeromonas caviae (33.3%), A. jandaei (38.3%) and A. veronii biovar sobria (28.3%). The strains isolated produced highly active hydrolytic enzymes, haemolytic activity and slime formation in varying proportions. The isolates were also tested for the enterotoxin genes (act, alt and ast), haemolytic toxins (hlyA and aerA), involved in type 3 secretion system (TTSS: ascV, aexT, aopP, aopO, ascF-ascG, and aopH), and glycerophospholipid-cholesterol acyltransferase (gcat). All isolates were found to be associated with at least one virulent gene. Moreover, they were resistant to frequently used antibiotics for human infections. The study demonstrates the pathogenic potential of Aeromonas, associated with ornamental fish culture systems suggesting the emerging threat to public health.
Descritores: Aciltransferases/análise
Aeromonas/genética
Aeromonas/isolamento & purificação
Suscetibilidade a Doenças
Resistência Microbiana a Medicamentos
Enterotoxinas/genética
Fauna Aquática/análise
Infecções por Bactérias Gram-Negativas
Técnicas In Vitro
Reação em Cadeia da Polimerase/métodos
Microbiologia da Água
-Ativação Enzimática
Peixes
Virulência
Amostras de Água
Limites: Humanos
Animais
Responsável: BR32.1 - Serviço de Biblioteca e Informação Biomédica


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Id: lil-326339
Autor: Antas, Paulo R. Z; Oliveira, Eliane B; Milagres, Alexandre S; Franken, Kees C; Ottenhoff, Tom H. M; Klatser, Paul; Sarno, Euzenir N; Sampaio, Elizabeth P.
Título: Kinetics of T cell-activation molecules in response to Mycobacterium tuberculosis antigens
Fonte: Mem. Inst. Oswaldo Cruz;97(8):1097-1099, Dec. 15, 2002. tab, graf.
Idioma: en.
Resumo: The phenotypic features acquired subsequent to antigen-specific stimulation in vitro were evaluated by means of the kinetic expressions of CD69 and CD25 activation molecules on T lymphocytes and assayed by flow cytometry in response to PPD, Ag85B, and ferritin in PPD-positive healthy control individuals. In response to PHA, CD69 staining on both CD4+ and CD8+ T cells became initially marked after 4 h, peaked at 24 h, and quickly decreased after 120 h. For CD25, a latter expression was detected around 8 h, having increased after 96 h. As expected, the response rate to the mycobacterial antigens was much lower than that to the mitogen. Positive staining was high after 96 h for CD25 and after 24 h for CD69. CD69 expression was significantly enhanced (p < 0.05) on CD8+ as compared to CD4+ T cells. High levels were also found between 96-120 h. Regarding Ag85B, CD25+ cells were mostly CD4+ instead of CD8+ T cells. Moreover, in response to ferritin, a lower CD25 expression was noted. The present data will allow further characterization of the immune response to new mycobacterial-specific antigens and their evaluation for possible inclusion in developing new diagnostic techniques for tuberculosis as well in a new vaccine to prevent the disease
Descritores: Antígenos de Bactérias
Antígenos CD
Antígenos de Diferenciação de Linfócitos T
Linfócitos T CD4-Positivos
Linfócitos T CD8-Positivos
Ativação Linfocitária/imunologia
Mycobacterium tuberculosis
Receptores de Interleucina-2
-Aciltransferases
Proteínas de Bactérias
Ferritinas
Citometria de Fluxo
Tuberculina
Responsável: BR1.1 - BIREME


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Id: lil-25098
Autor: Gillett, M. P; Oliveira, E. A.
Título: Altered erythrocyte and platelet lipid compositions in relation to lecithin: cholesterol acyltransferase deficiency in hepatosplenic schistosomiasis mansoni.
Fonte: Arq. biol. tecnol;27(4):511-20, 1984.
Idioma: en.
Descritores: Aciltransferases
Eritrócitos
Lipídeos
Fosfatidilcolina-Esterol O-Aciltransferase
Esquistossomose
-Hepatopatias
Esplenopatias
Limites: Humanos
Feminino
Responsável: BR1.1 - BIREME



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