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Pesquisa : D08.811.913.050.134.127 [Categoria DeCS]
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Id: lil-743755
Autor: Abou-Easa, Khalil; El-Magd, Mohammed Abu; Tousson, Ehab; Hassanin, Amin; Shukry, Mustafa; Salama, Mohammed.
Título: Pineal gland plays a role in gonadal development after eyelids separation in puppies / La glándula pineal juega un rol en el desarrollo gonadal luego de la separación de los párpados en cachorros
Fonte: Int. j. morphol;33(1):7-18, Mar. 2015. ilus.
Idioma: en.
Resumo: Several functional and morphological studies have been conducted on the pineal gland in many mammalian species; however, no published reports are available on the role of pineal gland on the gonadal development before and after eyelids separation in puppies. Therefore, this study aimed to trace the postnatal histo-morphological changes in the pineal gland and gonads of puppies before (2, 10 and 11 days old) and after (25, 35 and 40 days old) eyelids separation in an attempt to investigate the possible role of pineal gland on the gonadal development. In general, the differentiation of pineal cells, interstitial endocrine cells of testes and stromal ovarian cells coincides with the start of eyelids separation in puppies. Histological examination of stained pineal and gonadal slices of puppies after eyelids separation revealed a remarkable differentiation of pinealocytes and testicular interstitial endocrine cells, as well as presence of some evidence of folliculogenesis in ovary. Surprisingly, melatonin receptor (MT1) protein expression levels were significantly increased in the ovaries and testes of puppies after eyelids separation. Moreover, the mRNA and protein expression of AANAT, a rate-limiting enzyme in melatonin biosynthesis, was notably increased in the pineal gland of opened eyes puppies. Our results suggest an increase of melatonin production from the pineal gland of opened eyes puppies and this could play a vital role in the developmental changes observed in the gonads of these puppies.

Diversos estudios morfológicos y funcionales han sido realizados sobre la glándula pineal en distintas especies de mamíferos. Sin embargo, no hay informes publicados acerca del rol de la glándula pineal en el desarrollo gonadal antes y después de la separación de los párpados en cachorros. Este estudio tuvo como objetivo trazar los cambios histo-morfológicos postnatales en la glándula pineal y las gónadas de los cachorros antes (2, 10 y 11 días de edad) y después (25, 35 y 40 días de edad) de la separación de los párpados, en un intento por investigar el posible rol de la glándula pineal en el desarrollo gonadal. En general, la diferenciación de los pinealocitos, células intersticiales endocrinas de los testículos y las células estromales del ovario coincide con el inicio de la separación de los párpados en cachorros. El examen histológico de glándula pineal y los cortes gonadales de los cachorros, después de la separación de los párpados, reveló una notable diferenciación de los pinealocitos y las células intersticiales endocrinas testiculares, así como la posible evidencia de foliculogénesis en el ovario. Sorprendentemente, en el receptor de melatonina (MT1) los niveles de expresión de proteínas fueron significativamente superiores en los ovarios y los testículos de los cachorros después de la separación de los párpados. Además, el ARNm y la expresión de la proteína AANAT, una enzima limitante de la velocidad en la biosíntesis de la melatonina, aumentaron notablemente en la glándula pineal de los cachorros con los ojos abiertos. Nuestros resultados sugieren que existe un aumento de la producción de melatonina por parte de la glándula pineal en los cachorros con los ojos abiertos, lo que podría jugar un rol vital en los cambios evolutivos observados enlas gónadas de estos cachorros.
Descritores: Pálpebras/cirurgia
Gônadas/crescimento & desenvolvimento
Glândula Pineal/anatomia & histologia
Glândula Pineal/fisiologia
-Arilalquilamina N-Acetiltransferase/genética
Arilalquilamina N-Acetiltransferase/fisiologia
Western Blotting
Gônadas/anatomia & histologia
Melatonina/fisiologia
Reação em Cadeia da Polimerase
Transcrição Reversa
Limites: Animais
Masculino
Feminino
Cães
Responsável: CL1.1 - Biblioteca Central


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Id: lil-640542
Autor: Ding-ping, Bai; Cheng-jiang, Yu; Yu-lin, Chen.
Título: Association between AA-NAT gene polymorphism and reproductive performance in sheep
Fonte: Electron. j. biotechnol;15(2):7-7, Mar. 2012. ilus, tab.
Idioma: en.
Projeto: Doctor Foundation Items of Xinjiang Production & Construction Corps of China.
Resumo: Arylalkylamine N-acetyltransferase (AA-NAT) is critical enzyme in Melatonin (MLT) biosynthesis for MLT regulating the animal seasonal breeding. In this study, DNA sequencing methods were applied to detect the polymorphisms of the AA-NAT gene in 179 Chinese sheep belonging to two non-seasonal reproduction breeds and two seasonal reproduction breeds. One mutation at exon 3 (NM_001009461:c.486A > G) was firstly described at the sheep AA-NAT locus. Hence, we described the SmaI PCR-RFLP method for detecting EX3 486A > G mutation, frequencies of the AA-NAT-G allele varied from 0.871 to 0.908 in two non-seasonal reproduction breeds and 0.517 to 0.578 in two seasonal reproduction breeds. The associations of SmaI polymorphism with estrus traits was analyzed in non-seasonal reproduction breeds sheep and seasonal reproduction breeds sheep, the significant statistical results were found between them, the GG genotype frequencies was higher in non-seasonal reproduction breeds (p < 0.001), while, the GA genotype frequencies was higher in seasonal reproduction breeds (p < 0.05). Hence, the EX3 486A > G mutation could facilitate association analysis and serve as a genetic marker for Chinese sheep breeding and genetics.
Descritores: Arilalquilamina N-Acetiltransferase/genética
Ovinos/fisiologia
Ovinos/genética
-Arilalquilamina N-Acetiltransferase/fisiologia
Reação em Cadeia da Polimerase
Polimorfismo Genético
Polimorfismo de Fragmento de Restrição
Reprodução/fisiologia
Estações do Ano
Análise Estatística
Limites: Animais
Responsável: CL1.1 - Biblioteca Central



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