Base de dados : LILACS
Pesquisa : D08.811.913.050.134.180 [Categoria DeCS]
Referências encontradas : 7 [refinar]
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  1 / 7 LILACS  
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Texto completo SciELO Brasil
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Id: lil-464306
Autor: Lima, I. P; Saito, K; Hamassaki, D. E; Yan, C. Y. I.
Título: Immunohistochemical characterization of the chick marginal retina
Fonte: Braz. j. med. biol. res = Rev. bras. pesqui. méd. biol;40(11):1455-1464, Nov. 2007. ilus, tab.
Idioma: en.
Projeto: FAPESP; . CAPES.
Resumo: The retina is a highly differentiated tissue with a complex layered structure that has been extensively characterized. However, most of the previous studies focused on the histology of the central retina while little is known about the cellular composition, organization and function of the marginal retina. Recent research has identified a subpopulation of multipotential progenitor cells in the marginal regions of the retina, closest to the ciliary body ("ciliary marginal zone"). These cells are capable of differentiation in response to an appropriate stimulus. Thus, it is possible that the structure and composition of the marginal retina are distinct from those of the central retina to accommodate the potential addition of newly formed neurons. To characterize the cellular profile of the chick marginal retina, we labeled it immunohistochemically for markers whose staining pattern is well established in the central retina: calbindin, calretinin, protein kinase C, and choline acetyltransferase. Calbindin was present at very low levels in the marginal retina putative photoreceptor layer. Calretinin-positive horizontal cells were also sparse close to the ciliary marginal zone. The bipolar cells in the marginal outer plexiform layer were positive for anti-protein kinase C antibodies, but the density of labeling was also decreased in relation to the central retina. In contrast, the marginal starburst cholinergic amacrine cell pattern was very similar to the central retina. From these data we conclude that the structure of the marginal retina is significantly different from that of the central retina. In particular, the expression of late retina markers in the marginal retina decreased in comparison to the central retina.
Descritores: Corpo Ciliar/citologia
Proteínas do Olho/análise
Retina/química
Células Ganglionares da Retina/citologia
-Animais Recém-Nascidos
Biomarcadores/análise
Proliferação Celular
Galinhas
Colina O-Acetiltransferase/análise
Imuno-Histoquímica
Proteína Quinase C/análise
Retina/citologia
Retina/enzimologia
/análise
SACETOLACTATE SYNTHASE CALCIUM BINDING PROTEIN G/análise
Limites: Animais
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


  2 / 7 LILACS  
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Id: lil-269385
Autor: Nunes-Tavares, Nilson; Cunha-e-Silva, Narcisa Leal; Hassón-Voloch, Aída.
Título: Choline acetyltransferase detection in normal and denervated electrocyte from Electrophorus electricus (L.) using a confocal scanning optical microscopy analysis
Fonte: An. acad. bras. ciênc;72(3):331-40, Sept. 2000. ilus, tab.
Idioma: en.
Resumo: Acetylcholine is the neurotransmitter responsible for the transmission of impulses from cholinergic neurons to cells of innervated tissues. Its biosynthesis is catalyzed by the enzyme Choline acetyltransferase that is considered to be a phenotypically specific marker for cholinergic system. It is well known that the regulation of Choline acetyltransferase activity under physiological and pathological conditions is important for development and neuronal activities of cholinergic functions. We observed the distribution of Choline acetyltransferase in sections from the normal and denervated main electric organ sections of Electrophorus electricus (L.) by immunofluorescence using a anti-Choline acetyltransferase antibody. The animals were submitted to a surgical procedure to remove about 20 nerves and after 30 and 60 days, they were sacrificed. After 30 days, the results from immunohistochemistry demonstrated an increase on the Choline acetyltransferase distribution at denervated tissue sections when compared with the sections from the normal contralateral organ. A very similar labeling was observed between normal and denervated tissue sections of the animals after 60 days. However, Choline acetyltransferase activity (nmolesACh/ min/ mg of protein) in extracts obtained from electrocyte microsomal preparation, estimated by Fonnun's method (Fonnun 1975), was 70 per cent lower in the denervated extracts.
Descritores: Colina O-Acetiltransferase/metabolismo
Denervação
Electrophorus/metabolismo
-Colina O-Acetiltransferase/análise
Microscopia Confocal/métodos
Limites: Animais
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


  3 / 7 LILACS  
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Id: lil-224786
Autor: Bergado, Jorge A; Moreno, Heriberto; Soto, Johanka; Castellano, Orlando; Castillo, Lázara.
Título: Deptal fetal tissue transplants restore long-term potentiation in the dentate gyrus of fimbria-fornix-lesioned rats.
Fonte: La Habana; s.n; 1997. 10 p. ilus, tab, graf.
Idioma: en.
Resumo: Two groups of Sgrague-Dawley male rats received bilateral aspirative lesions of the fimbria fornix under chloral hydrate anesthesia. One group (n=9) received no further treatment (lesioned). In the second group (n=8), a piece of septal fetal tissue, obtained at day E15-16, was implanted into each lesion cavity (transplanted). A third group consisted of sham-lesioned rats (controls, n=14). Two months after the operations, a recording electrode was implanted in the hilar region of the dentate gyrus of each animal, and a bipolar stimulating electrode was implanted in the perforant path. Long-term potentiation at 400 Hz was induced and followed for two hours. FF-lesioned rats showed and impaired potentiation of the field excitatory post-synaptic potential, which rapidly declined to basal levels within 15 minutes. The transplanted rats showed a normal potentiation of this parameter, similar to that seen in the control animals. A decrease in choline acetyltransferase activity in the hippocampi of the lesioned animals showed a tendency toward recovery after septal fetal tissue transplantation. In all the dorsal hippocampal areas of the lesioned animals, acetylcholinesterase histochemistry showed an almost complete loss of enzymatic activity, which was partially restored by the transplants. The improved synaptic plasticity in the transplanted animals might be related to septal transplant-induced recovery of mnemonic functions
Descritores: Acetilcolinesterase
Colina O-Acetiltransferase
Hipocampo
Ratos
Transplante de Tecido Fetal
-Modelos Animais de Doenças
Limites: Animais
Responsável: CU414.1 - CIREN - Centro Internacional de Restauración Neurológica
CU414.1


  4 / 7 LILACS  
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Id: lil-223637
Autor: Fernández, C. I; Soto, J; González, O; González, M. E; Quijano, Z.
Título: Neurorestorative techniques as experimental approach to Alzheimer disease treatment.
Fonte: La Habana; s.n; 1995. 4 p. tab, graf.
Idioma: en.
Resumo: Laboratory animals have been used to reproduce some structural changes and/or memory impairment observed in Alzheimer disease by means of specific lesions or using old animals (Kordower and Gash, 1986). Different sources and places for the neural graft have been reported showing the graft's ability to attain an adequate and specific innervation of the target as well as the behavioral recovery (Dunnett, 1991; Gage and Chen, 1992). Similar experimental procedures have been used to evaluate effects of exogenous nerve growth factor (NGF) infusion, whose influence on central cholinergic neurons is well documented (Gage et al. 1991; Pepeu et al., 1993)
Descritores: Doença de Alzheimer/terapia
Colina O-Acetiltransferase
Hipocampo
Fatores de Crescimento Neural
Limites: Animais
Responsável: CU414.1 - CIREN - Centro Internacional de Restauración Neurológica
CU414.1


  5 / 7 LILACS  
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Id: lil-77418
Autor: Machado, C. R. S; Gomez, M. V; Machado, A. B. M.
Título: Changes in choline acetyltransferase activity of rat tissues during Chagas' disease
Fonte: Braz. j. med. biol. res = Rev. bras. pesqui. méd. biol;20(6):697-702, 1987. tab.
Idioma: en.
Projeto: CNPq.
Resumo: 1. The activity of choline acetyltransferase (CAT) measured by a radilabeling method was significantly reduced in the heart, submandibular gland and esophagus of rats 20 days after inoculation with Trypanosoma cruzi (Y strain). 2. Normal enzyme activity was recovered in all these organs 100 days after inoculation. 3. In the transcerse colon, no change, 30% reduction and normal activity were found at days 20, 100 and 430 of infection, respectively. 4. The data indicate recovery of parasympathetic function in experimental Chagas' disease. Axonal regrowth and sprouting are discussed as possible candidates for the recovery mechanisms
Descritores: Doença de Chagas/enzimologia
Colina O-Acetiltransferase/metabolismo
Esôfago/enzimologia
Glândula Submandibular/enzimologia
Miocárdio/enzimologia
Sistema Nervoso Parassimpático/fisiopatologia
-Colo/enzimologia
Regeneração Nervosa
Plasticidade Neuronal
Neurônios/fisiologia
Ratos Endogâmicos
Limites: Ratos
Animais
Masculino
Feminino
Responsável: BR26.1 - Biblioteca Central


  6 / 7 LILACS  
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Id: lil-70046
Autor: Nicolini, Humberto; Ortega, Hector; Fuente, Juan Ramon de la.
Título: Aspectos bioquimicos y etiopatogenicos de la demencia senil tipo Alzheimer
Fonte: Acta psiquiátr. psicol. Am. Lat;34(4):343-8, dic. 1988.
Idioma: es.
Descritores: Doença de Alzheimer/etiologia
-Acetilcolinesterase/deficiência
Alumínio/efeitos adversos
Encéfalo/enzimologia
Colina O-Acetiltransferase/deficiência
Príons/patogenicidade
Limites: Seres Humanos
Tipo de Publ: Revisão
Responsável: BR1.1 - BIREME


  7 / 7 LILACS  
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Id: lil-12908
Autor: Sarmiento Munevar, J.
Título: Neurotransmisores y envejecimiento. / Neurotransmitters and aging
Fonte: Neurol. Colomb;6(2):43-50, 1982.
Idioma: es.
Descritores: Envelhecimento
Doença de Alzheimer
Colina O-Acetiltransferase
Neurotransmissores
Limites: Idoso
Seres Humanos
Responsável: BR1.1 - BIREME



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