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Pesquisa : D08.811.913.050.134.415.500 [Categoria DeCS]
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Texto completo SciELO Chile
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Id: biblio-1011422
Autor: Wei, Fan; Tang, Danfeng; Li, Zengqiang; Kashif, Muhammad Haneef; Khan, Aziz; Lu, Hai; Jia, Ruixing; Chen, Peng.
Título: Molecular cloning and subcellular localization of six HDACs and their roles in response to salt and drought stress in kenaf (Hibiscus cannabinus L)
Fonte: Biol. Res;52:20, 2019. tab, graf.
Idioma: en.
Projeto: National Natural Science Foundation of China; . Modern Agro-industry Technology Research System.
Resumo: BACKGROUND: Histone acetylation is an important epigenetic modification that regulates gene activity in response to stress. Histone acetylation levels are reversibly regulated by histone acetyltransferases (HATs) and histone deacetylases (HDACs). The imperative roles of HDACs in gene transcription, transcriptional regulation, growth and responses to stressful environment have been widely investigated in Arabidopsis. However, data regarding HDACs in kenaf crop has not been disclosed yet. RESULTS: In this study, six HDACs genes (HcHDA2, HcHDA6, HcHDA8, HcHDA9, HcHDA19, and HcSRT2) were isolated and characterized. Phylogenetic tree revealed that these HcHDACs shared high degree of sequence homology with those of Gossypium arboreum. Subcellular localization analysis showed that GFP-tagged HcHDA2 and HcHDA8 were predominantly localized in the nucleus, HcHDA6 and HcHDA19 in nucleus and cytosol. The HcHDA9 was found in both nucleus and plasma membranes. Real-time quantitative PCR showed that the six HcHDACs genes were expressed with distinct expression patterns across plant tissues. Furthermore, we determined differential accumulation of HcHDACs transcripts under salt and drought treatments, indicating that these enzymes may participate in the biological process under stress in kenaf. Finally, we showed that the levels of histone H3 and H4 acetylation were modulated by salt and drought stress in kenaf. CONCLUSIONS: We have isolated and characterized six HDACs genes from kenaf. These data showed that HDACs are imperative players for growth and development as well abiotic stress responses in kenaf.
Descritores: Estresse Fisiológico/fisiologia
Hibiscus/enzimologia
Histona Acetiltransferases/fisiologia
Secas
Histona Desacetilases/fisiologia
-Ativação Transcricional/fisiologia
Clonagem Molecular
Hibiscus/crescimento & desenvolvimento
Hibiscus/fisiologia
Reação em Cadeia da Polimerase em Tempo Real
Responsável: CL1.1 - Biblioteca Central


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Id: lil-606641
Autor: Pierce, Raymond John; Dubois-Abdesselem, Florence; Caby, Stéphanie; Trolet, Jacques; Lancelot, Julien; Oger, Frédérik; Bertheaume, Nicolas; Roger, Emmanuel.
Título: Chromatin regulation in schistosomes and histone modifying enzymes as drug targets
Fonte: Mem. Inst. Oswaldo Cruz;106(7):794-801, Nov. 2011. ilus.
Idioma: en.
Resumo: Only one drug is currently available for the treatment and control of schistosomiasis and the increasing risk of selecting strains of schistosome that are resistant to praziquantel means that the development of new drugs is urgent. With this objective we have chosen to target the enzymes modifying histones and in particular the histone acetyltransferases and histone deacetylases (HDAC). Inhibitors of HDACs (HDACi) are under intense study as potential anti-cancer drugs and act via the induction of cell cycle arrest and/or apoptosis. Schistosomes like other parasites can be considered as similar to tumours in that they maintain an intense metabolic activity and rate of cell division that is outside the control of the host. We have shown that HDACi can induce apoptosis and death of schistosomes maintained in culture and have set up a consortium (Schistosome Epigenetics: Targets, Regulation, New Drugs) funded by the European Commission with the aim of developing inhibitors specific for schistosome histone modifying enzymes as novel lead compounds for drug development.
Descritores: Cromatina/efeitos dos fármacos
Inibidores Enzimáticos/farmacologia
Histona Acetiltransferases/antagonistas & inibidores
Histona Desacetilases/metabolismo
Schistosoma/efeitos dos fármacos
-Cromatina/metabolismo
Desenho de Fármacos
Histona Acetiltransferases/metabolismo
Inibidores de Histona Desacetilases/farmacologia
Schistosoma/enzimologia
Limites: Animais
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME



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