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Id: biblio-843478
Autor: Jaenisch, Rodrigo Boemo; Bertagnolli, Mariane; Borghi-Silva, Audrey; Arena, Ross; Lago, Pedro Dal.
Título: Respiratory muscle training improves diaphragm citrate synthase activity and hemodynamic function in rats with heart failure
Fonte: Rev. bras. cir. cardiovasc = Braz. j. cardiovasc. surg. (impr.);32(2):104-110, Mar.-Apr. 2017. tab, graf.
Idioma: en.
Resumo: Abstract INTRODUCTION: Enhanced respiratory muscle strength in patients with heart failure positively alters the clinical trajectory of heart failure. In an experimental model, respiratory muscle training in rats with heart failure has been shown to improve cardiopulmonary function through mechanisms yet to be entirely elucidated. OBJECTIVE: The present report aimed to evaluate the respiratory muscle training effects in diaphragm citrate synthase activity and hemodynamic function in rats with heart failure. METHODS: Wistar rats were divided into four experimental groups: sedentary sham (Sed-Sham, n=8), trained sham (RMT-Sham, n=8), sedentary heart failure (Sed-HF, n=7) and trained heart failure (RMT-HF, n=7). The animals were submitted to a RMT protocol performed 30 minutes a day, 5 days/week, for 6 weeks. RESULTS: In rats with heart failure, respiratory muscle training decreased pulmonary congestion and right ventricular hypertrophy. Deleterious alterations in left ventricular pressures, as well as left ventricular contractility and relaxation, were assuaged by respiratory muscle training in heart failure rats. Citrate synthase activity, which was significantly reduced in heart failure rats, was preserved by respiratory muscle training. Additionally, a negative correlation was found between citrate synthase and left ventricular end diastolic pressure and positive correlation was found between citrate synthase and left ventricular systolic pressure. CONCLUSION: Respiratory muscle training produces beneficial adaptations in the diaphragmatic musculature, which is linked to improvements in left ventricular hemodynamics and blood pressure in heart failure rats. The RMT-induced improvements in cardiac architecture and the oxidative capacity of the diaphragm may improve the clinical trajectory of patients with heart failure.
Descritores: Exercícios Respiratórios/métodos
Diafragma/enzimologia
Citrato (si)-Sintase/metabolismo
Insuficiência Cardíaca/enzimologia
Insuficiência Cardíaca/fisiopatologia
Hemodinâmica/fisiologia
-Pressão Sanguínea/fisiologia
Diafragma/fisiologia
Mecânica Respiratória/fisiologia
Ratos Wistar
Modelos Animais
Infarto do Miocárdio/fisiopatologia
Limites: Animais
Masculino
Responsável: BR1.1 - BIREME


  2 / 10 LILACS  
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Id: lil-755800
Autor: McIntosh, Douglas; Bezerra, Rodrigo Alves; Luz, Hermes Ribeiro; Faccini, João Luiz Horacio; Gaiotto, Fernanda Amato; Giné, Gastón Andrés Fernandez; Albuquerque, George Rego.
Título: Detection of Rickettsia bellii and Rickettsia amblyommii in Amblyomma longirostre (Acari: Ixodidae) from Bahia state, Northeast Brazil
Fonte: Braz. j. microbiol;46(3):879-883, July-Sept. 2015. tab.
Idioma: en.
Resumo:

Studies investigating rickettsial infections in ticks parasitizing wild animals in the Northeast region of Brazil have been confined to the detection of Rickettsia amblyommii in immature stages of Amblyomma longirostre collected from birds in the state of Bahia, and in immatures and females of Amblyomma auriculariumcollected from the striped hog-nosed skunk (Conepatus semistriatus) and armadillos (Euphractus sexcinctus) in the state of Pernambuco. The current study extends the distribution of R. amblyommii (strain Aranha), which was detected in A. longirostre collected from the thin-spined porcupine Chaetomys subspinosus and the hairy dwarf porcupine Coendou insidiosus. In addition, we report the first detection of Rickettsia bellii in adults of A. longirostre collected from C. insidiosus in the state of Bahia.

.
Descritores: Ixodidae/microbiologia
Infecções por Rickettsia/microbiologia
Rickettsia/genética
Rickettsia/isolamento & purificação
Infestações por Carrapato/microbiologia
-Animais Selvagens
Tatus
Sequência de Bases
Aves
Brasil
Proteínas da Membrana Bacteriana Externa/genética
Proteínas de Bactérias/genética
Citrato (si)-Sintase/genética
DNA Bacteriano
Proteínas de Choque Térmico/genética
Mephitidae
Dados de Sequência Molecular
Tipagem Molecular
Porcos-Espinhos
Proteínas Periplásmicas/genética
Análise de Sequência de DNA
Serina Endopeptidases/genética
Limites: Animais
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


  3 / 10 LILACS  
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Id: lil-572403
Autor: Venugopal, Vijay; Leggett, Robert E; Schuler, Catherine; Levin, Robert M.
Título: Effect of hydrogen peroxide on rabbit urinary bladder citrate synthase activity in the presence and absence of a grape suspension
Fonte: Int. braz. j. urol;36(6):749-758, Dec. 2010. graf.
Idioma: en.
Resumo: PURPOSE: The etiology of obstructive bladder dysfunction includes free radical damage to mitochondria. Feeding rabbits a standardized grape suspension protects the ability of the bladder to contract and empty in part by preventing mitochondrial damage, thus maintaining smooth muscle and mucosal metabolism. The objective of the current study is to determine the direct effect of this grape suspension on the response of mitochondria to the oxidative effects of hydrogen peroxide. MATERIALS AND METHODS: Six male rabbits were anesthetized with sodium pentobarbital and the bladders excised. Four full thickness strips were obtained for contractile studies and the balance separated into smooth muscle and mucosa compartments by blunt dissection. The effect of hydrogen peroxide on the contractile response to field stimulation was quantitated. Each tissue was homogenized and the effects of increasing concentrations of hydrogen peroxide in the presence and absence of grape suspension on citrate synthase activity was determined. RESULTS: Citrate synthase activity was significantly higher in the mucosa than in the muscle. The grape suspension had no effect on control citrate synthase activity. However, the grape suspension provided significant protection of both smooth muscle and mucosal citrate synthase activity. CONCLUSIONS: These studies support the conclusion that the grape suspension provides direct protection of mitochondrial function.
Descritores: Citrato (si)-Sintase/metabolismo
Peróxido de Hidrogênio/farmacologia
Mitocôndrias/metabolismo
Bexiga Urinária/efeitos dos fármacos
Vitis
-Antioxidantes/farmacologia
Peróxido de Hidrogênio/efeitos adversos
Membrana Mucosa/efeitos dos fármacos
Membrana Mucosa/enzimologia
Contração Muscular/efeitos dos fármacos
Músculo Liso/efeitos dos fármacos
Músculo Liso/enzimologia
Obstrução do Colo da Bexiga Urinária/enzimologia
Bexiga Urinária/enzimologia
Limites: Animais
Masculino
Coelhos
Tipo de Publ: Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Responsável: BR1.1 - BIREME


  4 / 10 LILACS  
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Id: lil-388061
Autor: Voces, J; Oliveira, A. C. Cabral de; Prieto, J. G; Vila, L; Perez, A. C; Duarte, I. D. G; Alvarez, A. I.
Título: Ginseng administration protects skeletal muscle from oxidative stress induced by acute exercise in rats
Fonte: Braz. j. med. biol. res = Rev. bras. pesqui. méd. biol;37(12):1863-1871, Dec. 2004. tab, graf.
Idioma: en.
Resumo: Enzymatic activity was analyzed in the soleus, gastrocnemius (red and white) and plantaris muscles of acutely exercised rats after long-term administration of Panax ginseng extract in order to evaluate the protective role of ginseng against skeletal muscle oxidation. Ginseng extract (3, 10, 100, or 500 mg/kg) was administered orally for three months to male Wistar rats weighing 200 ± 50 g before exercise and to non-exercised rats (N = 8/group). The results showed a membrane stabilizing capacity of the extract since mitochondrial function measured on the basis of citrate synthase and 3-hydroxyacyl-CoA dehydrogenase activities was reduced, on average, by 20 percent (P < 0.05) after exercise but the activities remained unchanged in animals treated with a ginseng dose of 100 mg/kg. Glutathione status did not show significant changes after exercise or treatment. Lipid peroxidation, measured on the basis of malondialdehyde levels, was significantly higher in all muscles after exercise, and again was reduced by about 74 percent (P < 0.05) by the use of ginseng extract. The administration of ginseng extract was able to protect muscle from exercise-induced oxidative stress irrespective of fiber type.
Descritores: Antioxidantes/farmacologia
Peroxidação de Lipídeos/efeitos dos fármacos
Mitocôndrias Musculares/efeitos dos fármacos
Músculo Esquelético/efeitos dos fármacos
Estresse Oxidativo/efeitos dos fármacos
Condicionamento Físico Animal
Panax/química
-/metabolismo
ABATTOIRS-HYDROXYACYL COA DEHYDROGENASES/metabolismo
Antioxidantes/administração & dosagem
Citrato (si)-Sintase/metabolismo
Glutationa/efeitos dos fármacos
Glutationa/metabolismo
Malondialdeído/análise
Mitocôndrias Musculares/metabolismo
Músculo Esquelético/metabolismo
Extratos Vegetais/farmacologia
Ratos Wistar
Superóxido Dismutase/metabolismo
Limites: Animais
Masculino
Ratos
Responsável: BR1.1 - BIREME


  5 / 10 LILACS  
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Casarini, D. E
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Id: lil-388058
Autor: Medeiros, A; Oliveira, E. M; Gianolla, R; Casarini, D. E; Negrão, C. E; Brum, P. C.
Título: Swimming training increases cardiac vagal activity and induces cardiac hypertrophy in rats
Fonte: Braz. j. med. biol. res = Rev. bras. pesqui. méd. biol;37(12):1909-1917, Dec. 2004. tab, graf.
Idioma: en.
Resumo: The effect of swimming training (ST) on vagal and sympathetic cardiac effects was investigated in sedentary (S, N = 12) and trained (T, N = 12) male Wistar rats (200-220 g). ST consisted of 60-min swimming sessions 5 days/week for 8 weeks, with a 5 percent body weight load attached to the tail. The effect of the autonomic nervous system in generating training-induced resting bradycardia (RB) was examined indirectly after cardiac muscarinic and adrenergic receptor blockade. Cardiac hypertrophy was evaluated by cardiac weight and myocyte morphometry. Plasma catecholamine concentrations and citrate synthase activity in soleus muscle were also determined in both groups. Resting heart rate was significantly reduced in T rats (355 ± 16 vs 330 ± 20 bpm). RB was associated with a significantly increased cardiac vagal effect in T rats (103 ± 25 vs 158 ± 40 bpm), since the sympathetic cardiac effect and intrinsic heart rate were similar for the two groups. Likewise, no significant difference was observed for plasma catecholamine concentrations between S and T rats. In T rats, left ventricle weight (13 percent) and myocyte dimension (21 percent) were significantly increased, suggesting cardiac hypertrophy. Skeletal muscle citrate synthase activity was significantly increased by 52 percent in T rats, indicating endurance conditioning. These data suggest that RB induced by ST is mainly mediated parasympathetically and differs from other training modes, like running, that seems to mainly decrease intrinsic heart rate in rats. The increased cardiac vagal activity associated with ST is of clinical relevance, since both are related to increased life expectancy and prevention of cardiac events.
Descritores: Frequência Cardíaca/fisiologia
Condicionamento Físico Animal/fisiologia
Natação/fisiologia
Sistema Nervoso Simpático/fisiologia
Nervo Vago/fisiologia
-Pressão Sanguínea/fisiologia
Bradicardia/etiologia
Bradicardia/fisiopatologia
Cardiomegalia/etiologia
Cardiomegalia/patologia
Catecolaminas/sangue
Citrato (si)-Sintase/metabolismo
Músculo Esquelético/enzimologia
Miócitos Cardíacos/metabolismo
Resistência Física/fisiologia
Ratos Wistar
Descanso/fisiologia
Fatores de Tempo
Limites: Animais
Masculino
Ratos
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


  6 / 10 LILACS  
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Id: lil-350460
Autor: Evangelista, F. S; Brum, P. C; Krieger, J. E.
Título: Duration-controlled swimming exercise training induces cardiac hypertrophy in mice
Fonte: Braz. j. med. biol. res = Rev. bras. pesqui. méd. biol;36(12):1751-1759, Dec. 2003. ilus, tab.
Idioma: en.
Projeto: FAPESP; . CNPq.
Resumo: Exercise training associated with robust conditioning can be useful for the study of molecular mechanisms underlying exercise-induced cardiac hypertrophy. A swimming apparatus is described to control training regimens in terms of duration, load, and frequency of exercise. Mice were submitted to 60- vs 90-min session/day, once vs twice a day, with 2 or 4 percent of the weight of the mouse or no workload attached to the tail, for 4 vs 6 weeks of exercise training. Blood pressure was unchanged in all groups while resting heart rate decreased in the trained groups (8-18 percent). Skeletal muscle citrate synthase activity, measured spectrophotometrically, increased (45-58 percent) only as a result of duration and frequency-controlled exercise training, indicating that endurance conditioning was obtained. In groups which received duration and endurance conditioning, cardiac weight (14-25 percent) and myocyte dimension (13-20 percent) increased. The best conditioning protocol to promote physiological hypertrophy, our primary goal in the present study, was 90 min, twice a day, 5 days a week for 4 weeks with no overload attached to the body. Thus, duration- and frequency-controlled exercise training in mice induces a significant conditioning response qualitatively similar to that observed in humans.
Descritores: Cardiomegalia
Condicionamento Físico Animal
Natação
-Pressão Sanguínea
Cardiomegalia
Citrato (si)-Sintase
Frequência Cardíaca
Camundongos Endogâmicos C57BL
Músculo Esquelético
Fatores de Tempo
Limites: Animais
Masculino
Camundongos
Responsável: BR1.1 - BIREME


  7 / 10 LILACS  
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Campello, Annibal P
Werneck, Lineu Cesar
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Id: lil-209180
Autor: Pedroso, Fábio Cesar; Campello, Annibal P; Werneck, Lineu Cesar; Klüppel, Maria Lúcia W.
Título: Caracterizaçäo de miopatias mitocondriais através da avaliaçäo das atividades enzimáticas envolvidas no metabolismo energético / Characterization of mitochondrial myopathies through the evaluation of the enzymatic activities involved in the energetic metabolism
Fonte: Arq. neuropsiquiatr;55(2):249-57, jun. 1997. tab.
Idioma: pt.
Resumo: Foi determinada a atividade das enzimas NADH desidrogenase, NADH citocromo e redutase, succinato desidrogenase, succinato citocromo e redutase, citocromo e oxidase e citrato sintase em mitocôndrias de músculo esquelético humano normal e doente (suspeito de miopatia mitocondrial). O grupo controle foi constituído de 13 indivíduos normais e que nao faziam uso contínuo de fármacos. O grupo doente era constituído de 10 pacientes cujo diagnóstico anatomopatológico indicava suspeita de miopatia mitocondrial. Observou-se reduçao na atividade das enzimas em todos os pacientes: 7 com anormalidades em todas as enzimas ensaiadas; 2 com deficiências em todas as enzimas exceto na citocromo e oxidase; e 1 paciente com disfunçao apenas na atividade da succinato desidrogenase e succinato citocromo e redutase. Este perfil possibilitou caracterizar múltiplas deficiências ou deficiência combinada da cadeia respiratória, além da disfunçao na citrato sintase em 9 pacientes. Um dos casos constituiu exceçao, sendo a deficiência enzimática restrita ao complexo II. Foi possível concluir que a metodologia usada é adequada e facilmente aplicável aos objetivos clínicos. Os resultados obtidos possibilitam a caracterizaçao dos complexos enzimáticos mitocondriais deficientes, mostrando que tais enfermidades sao originadas de disfunçao no metabolismo energético.
Descritores: Técnicas In Vitro
Mitocôndrias Musculares/enzimologia
Miopatias Mitocondriais/enzimologia
Músculo Esquelético/patologia
-Citrato (si)-Sintase/análise
Complexo IV da Cadeia de Transporte de Elétrons/análise
NADH Desidrogenase/análise
Succinato Citocromo c Oxirredutase/análise
Succinato Desidrogenase/análise
Limites: Humanos
Masculino
Feminino
Pré-Escolar
Criança
Adolescente
Adulto
Pessoa de Meia-Idade
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


  8 / 10 LILACS  
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Bicudo, J. E. P. W
Id: lil-161513
Autor: Bicudo, J. E. P. W; Zerbinatti, C. V.
Título: Physiological constraints in the aerobic performance of hummingbirds
Fonte: Braz. j. med. biol. res = Rev. bras. pesqui. méd. biol;28(11/12):1139-45, Nov.-Dec. 1995. tab.
Idioma: en.
Conferência: Apresentado em: International Symposium on Regulatory Mechanisms of Cardiovascular and Respiratory Function in Vertebrates, Säo Carlos, Sept. 8-13, 1994.
Projeto: FAPESP; . CNPq; . CAPES; . NIH.
Resumo: Hovering flight has been described as the most energetically expensive form of locomotion. Among the vertebrates, hummingbirds weighing only 1.5-20 g are the elite practitioners of this aerial art. Their flight muscles are, therefore, the most oxygen demanding locomotor muscles per unit tissue mass of all vertebrates. Tissue level functional and structural adaptations for oxygen transport are compared between hummingbirds and mammals in this paper. Hummingbirds present extreme structural adaptations in their flight muscles. Mitochondrial densities greater than 30 per cent are observed in their pectoral muscles, and the surface area of the inner membrane of their mitochondria is tvace that of mammals. This doubling of their mitochondrial oxidative capacity is accompanied by a proportional increase in the specific activity (per g tissue) of the mitochondrial manganese superoxide dismutase (SOD-Mn) in their flight muscles, thus indicating that oxygen toxicity is not a constraint in the aerobic performance of hummingbirds during hovering flight. Finally, the liver appears to play a major role in providing the necessary substrates for their high aerobic performance, and also in eliminating the oxygen free radicals formed during oxidative phosphorylation.
Descritores: Citrato (si)-Sintase/metabolismo
Consumo de Oxigênio/fisiologia
Voo Animal/fisiologia
Mitocôndrias Musculares/fisiologia
-Aves/fisiologia
Fosforilação Oxidativa
Limites: Animais
Tipo de Publ: Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.
Responsável: BR1.1 - BIREME


  9 / 10 LILACS  
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Id: lil-95037
Autor: Soares, José Manuel da Costa.
Título: Esforço contínuo vs esforço intermitente: adaptaçäo bioquímica / Continous exercise vs intermittent exercise: biochemical adaptation
Fonte: Rev. bras. ciênc. mov;4(3):19-25, jul.-1990. tab.
Idioma: pt.
Resumo: Foi estudo o efeito crônico de dois tipos de exercício (intermitente e contínuo) sobre a atividade de algumas enzimas do músculo do ratinho. O programa de treinos teve a duraçäo de 6 meses, 5 dias por semana, e foi executado num tapete rolante adaptado para animais. O grupo sujeito a corrida intermitente realizou o exercício a uma velocidade de 975m/h com fases de esforço de 30 segundos e com períodos de recuperaçäo de igual duraçäo. O grupo sujetio a exercício contínuo realizou o protocolo com igual velocidade sem fases de recuperaçäo. Os dois protocolos tiveram a duraçäo de 2 x 30 minutos com intervalo de 10 minutos. Foi analisada a atividade enzimática da creatina-quinase (CK), da lactato-desidrogenase (LDH) e da citrato-sintetase (CS). Näo foi encontrada qualquer variaçiao signficativa entre os grupos exercitados, apesar de se ter verificado näo só uma descida da atividade da LDH e uma subida da atividade da CK nos grupos exercitados, mas também uma descida ligeiro da atividade da CS no grupo intermitente e um aumento da atividade da CS no grupo sujeito a esforço contínuo. Os resultados sugerem diferentes mecanismos da adaptaçäo enzimática aos diferentes programas de treino
Descritores: Citrato (si)-Sintase/metabolismo
Creatina Quinase/metabolismo
L-Lactato Desidrogenase/metabolismo
Limites: Ratos
Animais
Masculino
Responsável: BR1.1 - BIREME


  10 / 10 LILACS  
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Id: lil-44796
Autor: Girardi, Elena; Rodriguez de Lores Arnaiz, Georgina.
Título: Citrate Synthase ande lactate dehydrogenase activities in rat cerebral cortex following the administration of the convulsants bicuculline and 3-mercaptopropionic acid
Fonte: Acta physiol. pharmacol. latinoam;37(2):235-43, 1987. tab.
Idioma: en.
Resumo: La administración intraperitoneal de bicuculina (Bic) y ácido 3-mercaptopropiónico (MP) produce convulsiones generalizadas en animales de laboratorio. En este trabajo se estudió el efecto de estos convulsivantes sobre la actividad de la lactato deshidrogenasa y de citrato sintasa de corteza cerebral de rata. La Bic se administró en dosis de1.0 mg/Kg (subconvulsiva) y 7.5 mg/Kg (convulsiva) y el MP en dosis de 150 mg/Kg (convulsiva). La actividad de lactato deshidrogenasa en fracciones solubre y particulada de corteza cerebral no se modificó por la administración de Bic o MP. La actividad de citrato sintasa en homogeneizados de corteza cerebral aumentó alrededor del 40% por la administración de Bic en dosis subconvulsiva y convulsiva; un aumento semejante se encontró por la administración de MP. No se encontró modificación en la actividad de la enzima hepática, sugiriendo especificidad de tejido. La mayor actividad de citrato sintasa en homogeneizados de corteza cerebral encontrada luego de la administración de los convulsivantes se correlaciona con el aumento en los niveles de citrato cerebral descriptos en estados convulsivos
Descritores: /farmacologia
ACIDO ABATTOIRS-MERCAPTOPROPIONICO/farmacologia
Bicuculina/farmacologia
Citrato (si)-Sintase/metabolismo
Córtex Cerebral/enzimologia
-L-Lactato Desidrogenase/metabolismo
Ratos Wistar
Limites: Ratos
Animais
Masculino
Responsável: BR1.1 - BIREME



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