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Texto completo SciELO Brasil
Texto completo
Id: lil-450287
Autor: Rincón, Gonzalo; Armstrong, Eileen; Postiglioni, Alicia.
Título: Analysis of the population structure of Uruguayan Creole cattle as inferred from milk major gene polymorphisms
Fonte: Genet. mol. biol;29(3):491-495, 2006. tab.
Idioma: en.
Resumo: The ancestors of Uruguayan Creole cattle were introduced by the Spanish conquerors in the XVII century, following which the population grew extensively and became semi-feral before the introduction of selected breeds. Today the Uruguayan Creole cattle genetic reserve consists of 575 animals. We used the tetra primer amplification refractory mutation system polymerase chain reaction (ARMS-PCR) to analyze the kappa-casein, beta-casein, alphaS1-casein and alpha-lactoalbumin gene polymorphisms and restriction fragment length polymorphism PCR (RFLP-PCR) for the beta-lactoglobulin and the acylCoA:diacyl glycerol acyltransferase 1 (DGAT1) genes. The kappa-casein and beta-lactoglobulin genes presented very similar A and B allele frequencies, while the alphas1-casein and alpha-lactoalbumin gene B alleles showed much higher frequencies than the corresponding A alleles. The beta-casein B allele was not found in the population sampled. There was a very high frequency of the DGAT1 gene A allele which is associated with low milk fat content and high milk yield. All loci were in Hardy-Weinberg equilibrium and the level of heterozygosity agreed with the high genetic diversity observed in a previous analysis of this population. Preservation of the allelic richness observed in the Uruguayan Creole cattle should be considered for future dairy management and livestock genetic improvement. The results also emphasize the value of the tetra primers ARMS-PCR technique as a rapid, easy and economical way of genotyping cattle breeds for milk gene single nucleotide polymorphisms.
Descritores: Bovinos/genética
Diacilglicerol O-Aciltransferase/genética
Proteínas do Leite
Polimorfismo de Nucleotídeo Único
-Polimorfismo de Fragmento de Restrição
Limites: Animais
Responsável: BR26.1 - Biblioteca Central

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Verneque, R. S
Teodoro, R. L
Carvalho, M. R. S
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Id: lil-441042
Autor: Lacorte, G. A; Machado, M. A; Martinez, M. L; Campos, A. L; Maciel, R. P; Verneque, R. S; Teodoro, R. L; Peixoto, M. G. C. D; Carvalho, M. R. S.
Título: DGAT1 K232A polymorphism in Brazilian cattle breeds
Fonte: Genet. mol. res. (Online);5(3):475-482, 2006. ilus, tab.
Idioma: en.
Resumo: Recent reports identified DGAT1 (EC harboring a lysine to alanine substitution (K232A) as a candidate gene with a strong effect on milk production traits. Our objective was to estimate the frequency of the DGAT1 K232A polymorphism in the main Zebu and Taurine breeds in Brazil as well as in Zebu x Taurine crossbreds as a potential QTL for marker-assisted selection. Samples of 331 animals from the main Brazilian breeds, Nellore, Guzerat, Red Sindhi, Gyr, Holstein, and Gyr x Holstein F1 were genotyped for DGAT1 K232A polymorphism (A and K alleles) using the PCR-RFLP technique. The highest frequency of the A allele was found in the Holstein sample (73%) followed by Gyr x Holstein F1 (39%). Gyr and Red Sindhi showed low frequencies of A alleles (4 and 2.5%, respectively). The A allele was not found in the Nellore and Guzerat samples. Our results could be used to guide association studies between this locus and milk traits in these breeds.
Descritores: Bovinos/genética
Diacilglicerol O-Aciltransferase/genética
Polimorfismo Genético/genética
Locos de Características Quantitativas/genética
Frequência do Gene
Marcadores Genéticos
Reação em Cadeia da Polimerase
Polimorfismo de Fragmento de Restrição
Limites: Animais
Responsável: BR1.1 - BIREME

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