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Pesquisa : D08.811.913.400.450.460 [Categoria DeCS]
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Id: biblio-839507
Autor: BERTOLDO, Barbara Bellocchio; SILVA, Camilla Beatriz da; RODRIGUES, Denise Bertulucci Rocha; GERALDO-MARTINS, Vinicius Rangel; FERRIANI, Virginia Paes Leme; NOGUEIRA, Ruchele Dias.
Título: Comparisons of IgA response in saliva and colostrum against oral streptococci species
Fonte: Braz. oral res. (Online);31:e39, 2017. tab, graf.
Idioma: en.
Projeto: Conselho Nacional de Pesquisa.
Resumo: Abstract The present study compared IgA specificity against oral streptococci in colostrum and saliva samples. Sixty-two mother-and-child pairs were included; samples of colostrum (C) and saliva (MS) were collected from the mothers and saliva samples were collected from babies (BS). The specificity of IgA against Streptococcus mutans and S. mitis were analyzed by western blot. Only 30% of babies’ samples presented IgA reactivity to S. mutans, while 74 and 80% of MS and C, respectively, presented this response. IgA reactivity to S. mutans virulence antigens (Ag I/II, Gtf and GbpB) in positive samples showed differences between samples for Gtf and especially for GbpB (p < 0.05), but responses to Ag I/II were similar (p > 0.05). The positive response of Gtf-reactive IgA was different between C (90%) and MS (58%) samples (p < 0.05), but did not differ from BS (p > 0.05). GbpB was the least detected, with 48 and 26% of C and MS, and only 5% of BS samples presenting reactivity (p > 0.05). Eight percent of MS and C samples presented identical bands to SM in the same time-point. In conclusion, the differences of IgA response found between C and MS can be due to the different ways of stimulation, proliferation and transportation of IgA in those secretions. The colostrum has high levels of IgA against S. mutans virulence antigens, which could affect the installation and accumulation process of S. mutans, mainly by supplying anti-GbpB IgA to the neonate.
Descritores: Saliva/imunologia
Streptococcus mutans/imunologia
Imunoglobulina A Secretora/análise
Imunoglobulina A Secretora/imunologia
Colostro/imunologia
Streptococcus mitis/imunologia
-Saliva/microbiologia
Proteínas de Bactérias/análise
Proteínas de Bactérias/imunologia
Virulência
Ensaio de Imunoadsorção Enzimática
Glicoproteínas/análise
Glicoproteínas/imunologia
Western Blotting
Análise de Variância
Colostro/microbiologia
Glucosiltransferases/análise
Glucosiltransferases/imunologia
Mães
Formação de Anticorpos/imunologia
Antígenos de Bactérias/análise
Antígenos de Bactérias/imunologia
Limites: Humanos
Feminino
Recém-Nascido
Responsável: BR1.1 - BIREME


  2 / 17 LILACS  
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Id: biblio-997965
Autor: Achmad, Muhammad Harun; Ramadhany, Sri; Suryajaya, Filipus Eric.
Título: Streptococcus Colonial Growth of Dental Plaque Inhibition Using Flavonoid Extract of Ants Nest (Myrmecodia pendans): An in Vitro Study
Fonte: Pesqui. bras. odontopediatria clín. integr;19(1):4250, 01 Fevereiro 2019. tab, graf.
Idioma: en.
Resumo: Objective: To know the activity of resistance of flavonoid content in ant nest plant in decreasing the number of colonies S. mutans oral cavity of children as a medic herbal material. Material and Methods: The subjects were plaque sample of 20 children aged 7-12 years. Research begins by making toothpaste from ant nest extract. Samples of children's dental plaque were inserted into BHIB media, after which incubated for 24 hours, 1/10 dilution with BHIB media three times, followed by TYC media planting and incubation of anaerob with temperature 370C for 48 hours. After that then count the number of colonies of S. mutans. Results: On ethyl acetate extract of ant nest incubated at room temperature with concentration 20%, 40%, 60%, 80%, 100% obtained a decrease from each treatment amount of Streptococcus mutans colony on TYC media with median value of each treatment was 89, 67, 64, 61, 59 and 51 for the ethyl acetate fraction, and 62, 61, 60, 59, 49 at the ethanol fraction. There was no significant difference between the six concentration groups (p>0.05). Conclusion: Flavonoids extract of ant nest plants have growth barrier on Streptococcus mutans bacteria, the greater the concentration given the greater the number of S. mutans colony.
Descritores: Plantas Medicinais
Streptococcus mutans
Flavonoides
Técnicas In Vitro/métodos
Placa Dentária
-Glucosiltransferases
Indonésia
Limites: Humanos
Criança
Responsável: BR1264.1 - Biblioteca Setorial Prof Alberto M Campos


  3 / 17 LILACS  
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Id: lil-775124
Autor: Coelho, Sheila Lorena de Araújo; Magalhães, Valter Cruz; Marbach, Phellippe Arthur Santos; Cazetta, Marcia Luciana.
Título: A new alkalophilic isolate of Bacillus as a producer of cyclodextrin glycosyltransferase using cassava flour
Fonte: Braz. j. microbiol;47(1):120-128, Jan.-Mar. 2016. tab, graf.
Idioma: en.
Resumo: Abstract Cyclodextrin glycosyltransferase (CGTase) catalyzes the conversion of starch into non-reducing cyclic sugars, cyclodextrins, which have several industrial applications. This study aimed to establish optimal culture conditions for β-CGTase production by Bacillus sp. SM-02, isolated from soil of cassava industries waste water lake. The optimization was performed by Central Composite Design (CCD) 2, using cassava flour and corn steep liquor as substrates. The maximum production of 1087.9 U mL−1 was obtained with 25.0 g L−1 of cassava flour and 3.5 g L−1 of corn steep after 72 h by submerged fermentation. The enzyme showed optimum activity at pH 5.0 and temperature 55 °C, and maintained thermal stability at 55 °C for 3 h. The enzymatic activity was stimulated in the presence of Mg+2, Ca+2, EDTA, K+, Ba+2 and Na+ and inhibited in the presence of Hg+2, Cu+2, Fe+2 and Zn+2. The results showed that Bacillus sp. SM-02 have good potential for β-CGTase production.
Descritores: Bacillus/isolamento & purificação
Bacillus/metabolismo
Meios de Cultura/química
Glucosiltransferases/metabolismo
-Ativadores de Enzimas/metabolismo
Inibidores Enzimáticos/análise
Concentração de Íons de Hidrogênio
Manihot/metabolismo
Microbiologia do Solo
Temperatura
Zea mays/metabolismo
Responsável: BR1.1 - BIREME


  4 / 17 LILACS  
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Vicente, Vânia Aparecida
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Id: lil-725346
Autor: Neiva, Ivana Froede; Moreira, Mônica; Gomes, Renata Rodrigues; Klisiowicz, Debora; Souza, Ricardo Lehtonen Rodrigues; Vicente, Vânia Aparecida.
Título: Using molecular markers to assess Streptococcus mutans variability and the biological risk for caries
Fonte: Braz. j. oral sci;13(3):235-241, Jul-Sep/2014. tab, graf.
Idioma: en.
Resumo: AIM: To characterize the genetic variability of Streptococcus mutans isolates and to correlate this variability with different colonization profiles observed during dental caries in a sample of children. METHODS: S. mutans samples were isolated from the saliva of 30 children with varying histories of dental caries, and they were characterized according to morphological and biochemical markers and the sequences of their 16S-23S intergenic spacer region. The genetic variability of the isolates was first assessed using Random Amplified Polymorphic DNA (RAPD) markers. Next, the isolates were differentiated by sequencing a specific region of the gene encoding the enzyme glucosyltransferase B (gtfB). RESULTS: Characterization using RAPD markers uncovered significant genetic variability among the samples and indicated the existence of clusters, which allowed us to reconstruct both the origin and clinical history of the disease. By sequencing the 16S-23S intergenic region, it was found that all of the isolates belonged to the species S. mutans. Based on the genetic similarity of the isolates and pattern of amino acid variations identified by partial sequencing of the gtfB gene, base-pair changes were identified and correlated with different virulence patterns among the isolates. CONCLUSIONS: The partial sequencing of the gtfB gene can be a useful tool for elucidating the colonization patterns of S. mutans. As amino acid variations are likely to be correlated with differences in biological risk, molecular characterization, such as that described in this paper, could be the key for assessing the development of dental caries in children...
Descritores: Cárie Dentária/epidemiologia
Glucosiltransferases
Streptococcus mutans/genética
Técnica de Amplificação ao Acaso de DNA Polimórfico/métodos
Limites: Humanos
Masculino
Feminino
Criança
Responsável: BR218.1 - Biblioteca Carlos Henrique Robertson Liberalli


  5 / 17 LILACS  
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Id: lil-668286
Autor: Barrientos, Silvia; Rodríguez, Adriana.
Título: Production of glucosyltransferase B and glucans by streptococcus mutans strains isolated from caries-free individuals
Fonte: Acta odontol. latinoam;24(3):258-264, 2011.
Idioma: en.
Resumo: La glucosiltranferasa B es una enzima producida por Streptococcus mutans, que a partir de la sacarosa, cataliza la síntesis de glucanos insolubles los cuales dan soporte a la biopelícula, siendo uno de los principales factores de virulencia para la generación de la caries dental. Sin embargo, no se ha esclarecido su papel en los individuos libre de caries, portadores delmicroorganismo. El objetivo de este estudio fue determinar la producción de glucosiltransferasa B y la producción de glucanos por cepas de Streptococcus mutans aisladas de biopelícula de 30individuos libres de caries. Las cepas fueron cultivadas en caldo Todd Hewitt y las proteínas extracelulares fueron obtenidas por precipitación con sulfato de amonio las proteínas asociadas amembrana por extracción con urea. La presencia de GtfB fue determinada por peso molecular por SDS–PAGE, confirmada por Western Blot utilizando un anticuerpo específico y la producciónde polisacáridos por separación electroforética, incubación con sacarosa y coloración de Schiff. Los resultados muestran que el 96.7 por ciento de las cepas de Streptococcus mutans producen una banda a la altura del peso molecular correspondiente a las Gtf,de las cuales son reactivas por western blot el 63.4 por ciento El 93.3 por cientode las cepas producen polisacáridos. Conclusiones: la cepas de Streptococcus mutans aisladas de biopelícula de individuos sanos producen factores de virulencia asociados a la caries dental como glucosiltransferasa B y glucanos lo que indica que hay condiciones en la cavidad oral diferentes a estos factores que mantienen al individuo libre de caries dental, los cuales deben ser investigados en la búsqueda de estrategias para controlar la enfermedad.
Descritores: Biofilmes
Cárie Dentária/enzimologia
Glucosiltransferases/classificação
Streptococcus mutans/isolamento & purificação
-Western Blotting
Glucanos/fisiologia
Fatores de Virulência
Limites: Humanos
Responsável: AR29.1 - Biblioteca


  6 / 17 LILACS  
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Id: lil-657664
Autor: Courtois, Anthony; Gac-Breton, Stéphanie; Berthou, Christian; Guézennec, Jean; Bordron, Anne; Boisset, Claire.
Título: Complement dependent cytotoxicity activity of therapeutic antibody fragments is acquired by immunogenic glycan coupling
Fonte: Electron. j. biotechnol;15(5):5-5, Sept. 2012. ilus, tab.
Idioma: en.
Resumo: Oligosaccharides are implicated in the development of the immune response notably in complement activation. Anti-tumoural immunotherapy by monoclonal antibodies (mAbs) offers some advantages to chemotherapy including cell targeting but some of them are inefficient to generate cytotoxicity dependent complement (CDC) known to be important in the antibody's efficacy. The aim of this study is to give a CDC activity of mAb by linkage of a complement activating oligosaccharide to this antibody via a hetero-bifunctional linker allowing control of the conjugation reaction. We worked on non Hodgkin Burkitt's lymphoma as cancer source, Fab fragments of rituximab devoid of complement activity as mAb and the trisaccharide Gal alpha(1→3)Gal beta(1→4)GlcNAc as immunogenic glycan. The bioconjugate Fab-Gal was characterized by biochemical methods and we demonstrated that the α-Gal epitope was recognized by seric immunoglobulins. After checking the recognition capacity of the Fab-Gal conjugate for the CD20 epitope, in vitro assays were performed to evaluate the activation of the complement cascade by the Fab-Gal conjugate. The effect of this bioconjugate was confirmed by the evaluation of the proliferation response of Burkitt's cell line. The relative facility realization of this strategy represents new approaches to increase activities of mAbs.
Descritores: Antígenos Heterófilos
Citotoxicidade Imunológica
Glucosiltransferases/imunologia
Oligossacarídeos/imunologia
Proteínas do Sistema Complemento/imunologia
-Citometria de Fluxo
Imunoterapia
Linfoma não Hodgkin/imunologia
Responsável: CL1.1 - Biblioteca Central


  7 / 17 LILACS  
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Id: lil-640511
Autor: Ibrahim, Abdelnasser S. S; El-Tayeb, Mohamed A; Elbadawi, Yahya B; Al-Salamah, Ali A.
Título: Effects of substrates and reaction conditions on production of cyclodextrins using cyclodextrin glucanotransferase from newly isolated Bacillus agaradhaerens KSU-A11
Fonte: Electron. j. biotechnol;14(5):4-4, Sept. 2011. ilus, tab.
Idioma: en.
Resumo: The effects of reaction conditions on cyclodextrins (CDs) production by CGTase from newly isolated Bacillus agaradhaerens KSU-A11 is reported. Among six types of starch tested, potato starch gave highest starch conversion into CDs. In addition, CDs yield was about three fold higher when using gelatinized potato starch in comparison to raw starch. The total CDs production was increased with increasing pH, showing maximum starch conversion at pH 10. Furthermore, the proportion of gamma-CD was relatively higher under slightly acidic-neutral conditions than at alkaline pH with a maximum proportion of 35.6 percent at pH 7 compared to 7.6 percent at pH 10. Maximum starch conversion into CDs was seen at reaction temperature of 55ºC. Lower reaction temperature led to higher proportion of gamma-CD with maximum percentage at 35ºC. Cyclization reaction was significantly promoted in the presence CaCl2 (10 mM), while in the presence of ethyl alcohol there was significant decrease in CD production particularly at high concentration. beta-CD was the major product up to 1 hr reaction period with traces of alpha-CD and no detectable gamma-CD. However, as the reaction proceed, gamma-CD started to be synthesised and alpha-CD concentration increased up to 4 hrs, where the CDs ratios were 0.27:0.65:0.07 for alpha-CD:beta-CD:gamma-CD, respectively. In addition, optimum CGTase/starch ratio was obtained at 80 U/g starch, showing highest starch conversion into CDs. All the parameters involved have been shown to affect the products yield and/or specificity of B. agaradhaerens KSU-A11 CGTase.
Descritores: Bacillus/isolamento & purificação
Bacillus/enzimologia
Ciclodextrinas/biossíntese
Glucosiltransferases/metabolismo
-Ativação Enzimática
Ensaios Enzimáticos
Concentração de Íons de Hidrogênio
Especificidade por Substrato
Temperatura
Responsável: CL1.1 - Biblioteca Central


  8 / 17 LILACS  
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Id: lil-586034
Autor: Rahim, Zubaidah Haji Abdul; Thurairajah, Nalina.
Título: Scanning Electron Microscopic study of Piper betle L. leaves extract effect against Streptococcus mutans ATCC 25175
Fonte: J. appl. oral sci;19(2):137-146, May-Apr. 2011. ilus, tab.
Idioma: en.
Resumo: INTRODUCTION: Previous studies have shown that Piper betle L. leaves extract inhibits the adherence of Streptococcus mutans to glass surface, suggesting its potential role in controlling dental plaque development. OBJECTIVES: In this study, the effect of the Piper betle L. extract towards S. mutans (with/without sucrose) using scanning electron microscopy (SEM) and on partially purifed cell-associated glucosyltransferase activity were determined. MATERIAL AND METHODS: S. mutans were allowed to adhere to glass beads suspended in 6 different Brain Heart Infusion broths [without sucrose; with sucrose; without sucrose containing the extract (2 mg mL-1 and 4 mg mL-1); with sucrose containing the extract (2 mg mL-1 and 4 mg mL-1)]. Positive control was 0.12 percent chlorhexidine. The glass beads were later processed for SEM viewing. Cell surface area and appearance and, cell population of S. mutans adhering to the glass beads were determined upon viewing using the SEM. The glucosyltransferase activity (with/without extract) was also determined. One- and two-way ANOVA were used accordingly. RESULTS: It was found that sucrose increased adherence and cell surface area of S. mutans (p<0.001). S. mutans adhering to 100 µm² glass surfaces (with/without sucrose) exhibited reduced cell surface area, fuffy extracellular appearance and cell population in the presence of the Piper betle L. leaves extract. It was also found that the extract inhibited glucosyltransferase activity and its inhibition at 2.5 mg mL-1 corresponded to that of 0.12 percent chlorhexidine. At 4 mg mL-1 of the extract, the glucosyltransferase activity was undetectable and despite that, bacterial cells still demonstrated adherence capacity. CONCLUSION: The SEM analysis confrmed the inhibitory effects of the Piper betle L. leaves extract towards cell adherence, cell growth and extracellular polysaccharide formation of S. mutans visually. In bacterial cell adherence, other factors besides glucosyltransferase are involved.
Descritores: Aderência Bacteriana/efeitos dos fármacos
Glucosiltransferases/antagonistas & inibidores
Piper betle
Extratos Vegetais/farmacologia
Streptococcus mutans/efeitos dos fármacos
-Análise de Variância
Placa Dentária/prevenção & controle
Vidro
Glucosiltransferases/efeitos dos fármacos
Microscopia Eletrônica de Varredura
Folhas de Planta
Estatísticas não Paramétricas
Propriedades de Superfície
Streptococcus mutans/crescimento & desenvolvimento
Sacarose/farmacologia
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


  9 / 17 LILACS  
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Id: lil-577109
Autor: Cheirsilp, Benjamas; Kitcha, Suleeporn; Maneerat, Supasilp.
Título: Kinetic characteristics of beta-cyclodextrin production by cyclodextrin glycosyltransferase from newly isolated Bacillus sp. C26
Fonte: Electron. j. biotechnol;13(4):4-5, July 2010. ilus, tab.
Idioma: en.
Projeto: Prince of Songkla University.
Resumo: The kinetic characteristics of beta-cyclodextrin production by a cyclodextrin glycosyltransferase (CGTase) produced by Bacillus sp. C26, a new isolate from a soil sample was investigated. Considering highest yield and initial production rate of beta-cyclodextrin, among the starches examined, soluble starch, tapioca starch, sago starch, corn starch and rice starch, tapioca starch was the best substrate for this CGTase. The optimum temperature for tapioca starch gelatinization prior to its use as a substrate for beta-cyclodextrin production was 65ºC. The yield and initial production rate of beta-cyclodextrin increased with increasing starch concentration up to 6 percent and an enzyme concentration up to 48 U/g-starch. The kinetic parameters of Vmax and Km of beta-cyclodextrin production from tapioca starch by CGTase were 1.59 mg/mL/h and 22.3 mg/mL, respectively. Considering high initial production rate and high yield of beta-cyclodextrin, the optimum reaction temperature was at 50ºC. This study provided the necessary kinetic information that may be useful to define the most suitable condition for industrialized production of beta-cyclodextrin with the high yield and productivity.
Descritores: Bacillus/enzimologia
Glucosiltransferases/metabolismo
Cinética
Leveduras/metabolismo
beta-Ciclodextrinas/metabolismo
-Bacillus/isolamento & purificação
Microbiologia do Solo
Temperatura
Responsável: CL1.1 - Biblioteca Central


  10 / 17 LILACS  
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Id: lil-442559
Autor: Okamoto, H. T; Soares, C. M; Pereira, M.
Título: Comparative analyses of the structure of the 1, 3-beta-glucan synthase gene in Paracoccidioides brasiliensis isolates
Fonte: Genet. mol. res. (Online);5(2):407-418, 2006. ilus.
Idioma: en.
Resumo: The evolutionary origin and significance of spliceosomal introns have been the subject of many investigations. Two theories, [quot ]introns-early[quot ] theory and [quot ]introns-late[quot ] theory, have been proposed to explain the evolution of introns in eukaryotic genes. Intron position is generally conserved in paralogue and orthologue genes. Some introns occur at similar but not necessarily identical positions in homologous genes, which were separated by great evolutionary distances. This event can be explained by insertion, loss or movement of the intron over short distances. Intron loss and gain events are unique in evolution and can be useful as markers for phylogenetic analyses. The insertion of introns at an identical position suggests a common ancestor gene. Here we analyzed, using PCR and RT-PCR, the structure of the 1,3-beta-glucan synthase gene (FKS) in several clinical isolates of Paracoccidioides brasiliensis (Pb): isolates Pb 01, Pb 4940, Pb 8515, Pb 8311, Pb 8334, Pb 4268, Pb 1668, and Pb E. Our results showed that seven of the isolates examined showed identical structures concerning the position of introns in PbFKS1. PbFKS4940 showed the intron described at the 3' end and had lost that one at the 5' end. The presence of the PbFKS4940 transcript suggests that it could be a functional gene. These data suggest a divergent evolution for introns with regard to the 1,3-beta-glucan synthase gene in P. brasiliensis isolates.
Descritores: DNA Fúngico/genética
Evolução Molecular
Glucosiltransferases/genética
Íntrons/genética
Paracoccidioides/genética
-Sequência de Aminoácidos
Sequência de Bases
Dados de Sequência Molecular
Filogenia
Reação em Cadeia da Polimerase Via Transcriptase Reversa
Responsável: BR1.1 - BIREME



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