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Pesquisa : D08.811.913.696.620.475 [Categoria DeCS]
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Id: lil-604286
Autor: Vaziri, Farzam; Peerayeh, Shahin Najar; Nejad, Qorban Behzadian; Farhadian, Abbas.
Título: The prevalence of aminoglycoside-modifying enzyme genes (aac (6')-I, aac (6')-II, ant (2")-I, aph (3')-VI) in Pseudomonas aeruginosa
Fonte: Clinics;66(9):1519-1522, 2011. ilus, tab.
Idioma: en.
Resumo: INTRODUCTION: Pseudomonas aeruginosa (P. aeruginosa) is one of the primary opportunistic pathogens responsible for nosocomial infections. Aminoglycosides are an import ant component of antipseudomonal chemotherapy. The inactivation of drugs by modifying enzymes is the most common mechanism of aminoglycoside resistance. OBJECTIVES: The inactivation of aminoglycosides by modifying enzymes is the primary resistance mechanism employed by P. aeruginosa. The aim of the present study was to investigate the occurrence of aminoglycoside resistance and the prevalence of four import ant modifying enzyme genes (aac (6')-I, aac (6')-II, ant (2")-I, aph (3')-VI) in P. aeruginosa in Iran. METHODS: A total of 250 clinical isolates of P. aeruginosa were collected from several hospitals in seven cities in Iran. Antimicrobial susceptibility tests (using the disk diffusion method and E-tests) were performed for all 250 isolates. In addition, all isolates were screened for the presence of modifying enzyme genes by polymerase chain reaction. RESULTS: The resistance rates, as determined by the disk diffusion method, were as follows: gentamicin 43 percent, tobramycin 38 percent, and amikacin 24 percent. Of the genes examined, aac (6')-II (36 percent) was the most frequently identified gene in phenotypic resist ant isolates, followed by ant (2")-I, aph (3')-VI, and aac (6')-I. CONCLUSIONS: Aminoglycoside resistance in P. aeruginosa remains a signific ant problem in Iran. Therefore, there is considerable local surveillance of aminoglycoside resistance.
Descritores: Acetiltransferases/genética
Aminoglicosídeos/farmacologia
Antibacterianos/farmacologia
Farmacorresistência Bacteriana/genética
Canamicina Quinase/genética
Nucleotidiltransferases/genética
Pseudomonas aeruginosa/genética
-Aminoglicosídeos/metabolismo
Antibacterianos/metabolismo
DNA Bacteriano/genética
Farmacorresistência Bacteriana/efeitos dos fármacos
Irã (Geográfico)
Pseudomonas aeruginosa/efeitos dos fármacos
Limites: Feminino
Humanos
Masculino
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


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Id: lil-251322
Autor: Santos, Wagner G. dos; Metcheva, Ivelina; Buck, Gregory A.
Título: Colony polymerase chain reaction of stably transfected Trypanosoma cruzi grown on solid medium
Fonte: Mem. Inst. Oswaldo Cruz;95(1):111-4, Jan.-Feb. 2000. ilus.
Idioma: en.
Resumo: Tools for the genetic manipulation of Trypanosoma cruzi are largely unavailable, although several vectors for transfection of epimastigotes and expression of foreign or recombinant genes have been developed. We have previously constructed several plasmid vectors in which recombinant genes are expressed in T. cruzi using the rRNA promoter. In this report, we demonstrate that one of these vectors can simultaneously mediate expression of neomycin phosphotransferase and green fluorescent protein when used to stably transfect cultured epimastigotes. These stably transfected epimastigotes can be selected and cloned as unique colonies on solid medium. We describe a simple colony PCR approach to the screening of these T. cruzi colonies for relevant genes. Thus, the methodologies outlined herein provide important new tools for the genetic dissection of this important parasite.
Descritores: Reação em Cadeia da Polimerase
Transfecção
Trypanosoma cruzi/genética
-Meios de Cultura
Primers do DNA
Genes Reporter
Genótipo
Canamicina Quinase
Trypanosoma cruzi/enzimologia
Trypanosoma cruzi/crescimento & desenvolvimento
Limites: Animais
Tipo de Publ: Research Support, U.S. Gov't, P.H.S.
Research Support, U.S. Gov't, Non-P.H.S.
Responsável: BR1.1 - BIREME



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