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Id: biblio-843639
Autor: Melo, Gleicia Martins de; Cardoso, Maria Vera Lúcia Moreira Leitão.
Título: Non-pharmacological measures in preterm newborns submitted to arterial puncture / Medidas no farmacológicas en recién nacidos prematuros sometidos a la punción arterial / Medidas não farmacológicas em recém-nascidos pré-termo submetidos à punção arterial
Fonte: Rev. bras. enferm;70(2):317-325, Mar.-Apr. 2017. tab.
Idioma: en.
Projeto: National Council for Scientific and Technological Development.
Resumo: ABSTRACT Objective: to assess pain in preterm newborns and to compare the neonatal and therapeutic variables with the total scores of the Neonatal Facial Coding System of preterm newborns submitted to arterial puncture exposed to music and 25% oral glucose. Method: a comparative study with 48 recordings of preterm newborns - Group 1, music (26); Group 2, glucose 25% (22) - individually analyzed by three trained nurses, after Kappa of at least 80%. Results: the variables and the pain scores of the groups did not present statistical significance (p < 0.05) according to the Neonatal Facial Coding System. 80.8% of the preterm infants in Group 1 had a higher quantitative score ≥ 3 in the neonatal variables (gender, type of delivery), and therapeutic variables (type of oxygen therapy, place of hospitalization, type of puncture). Conclusion: There was no difference when comparing the music and glucose 25% groups and the variables studied.

RESUMEN Objetivo: evaluar el dolor en recién nacidos prematuros y comparar las variables neonatales y terapéuticas con las puntuaciones totales del Neonatal Facial Coding System de los recién nacidos prematuros sometidos a una punción arterial expuestos a la música y glucosa al 25% por vía oral. Método: estudio comparativo con 48 fi lmaciones de los recién nacidos prematuros divididos en el Grupo 1 - música (26) y el Grupo 2 - glucosa al 25% (22). Las fi lmaciones fueron analizadas individualmente por tres enfermeras capacitadas después de coefi ciente Kappa de al menos 80%. Resultados: las variables y puntuaciones de dolor de los grupos no fueron estadísticamente signifi cativas (p<0,05) de acuerdo con el Neonatal Facial Coding System. En el Grupo 1, 80,8% de los recién nacidos prematuros mostraron mayores cantidades de puntuaciones ≥ 3 en las variables neonatales (sexo, tipo de parto) y las variables terapéuticas (tipo de la terapia de oxígeno, lugar de internación, tipo de punción). Conclusión: No hubo diferencias cuando se comparan los grupos de música y de glucosa al 25% y las variables estudiadas.

RESUMO Objetivo: avaliar a dor em recém-nascidos pré-termo e comparar as variáveis neonatais e terapêuticas com os escores totais da Neonatal Facial Coding System de recém-nascidos pré-termo submetidos à punção arterial exposto à música e glicose 25% oral. Método: estudo comparativo com 48 fi lmagens de recém-nascidos pré-termo - Grupo 1, música (26); Grupo 2, glicose 25% (22) - analisadas individualmente por três enfermeiras treinadas, após Kappa de no mínimo 80%. Resultados: as variáveis e os escores de dor dos grupos não apresentaram signifi cância estatística (p < 0,05) de acordo com o Neonatal Facial Coding System. 80,8% dos prematuros do Grupo 1 apresentaram um maior quantitativo de escores ≥ 3 nas variáveis neonatais (sexo, tipo de parto) e, variáveis terapêuticas (tipo de oxigenoterapia, local de internação, tipo de punção). Conclusão: Não houve diferença ao se comparar os grupos da música e da glicose 25% e as variáveis estudadas.
Descritores: Medição da Dor/métodos
Ferimentos Penetrantes Produzidos por Agulha/complicações
Manejo da Dor/normas
-Dor/enfermagem
Acetilglucosamina/uso terapêutico
Recém-Nascido Prematuro/psicologia
Recém-Nascido Prematuro/sangue
Manejo da Dor/métodos
Glucose/farmacologia
Musicoterapia/métodos
Musicoterapia/normas
Limites: Humanos
Masculino
Feminino
Recém-Nascido
Responsável: BR1.1 - BIREME


  2 / 11 LILACS  
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Id: lil-723953
Autor: Seckiner, Ilker; Bayrak, Omer; Can, Murat; Mungan, A. Gorkem; Mungan, N. Aydin.
Título: Garlic supplemented diet attenuates gentamicin nephrotoxicity ın rats
Fonte: Int. braz. j. urol;40(4):562-567, Jul-Aug/2014. tab.
Idioma: en.
Resumo: Purpose To demonstrate the effect of a 4% pulverized garlic supplemented diet on the nephrotoxicity induced by gentamicin in rats. Materials and Methods Twenty four healthy male Wistar rats, weighing between 220 - 260grams, were divided into three groups. The rats were randomly assigned to either the gentamicin injection without garlic supplementation group (Group I, n = 8), gentamicin injection with garlic supplementation group (Group II, n = 8), and control group (Group III, n = 8). Urine from the rats was collected and the volume (mL), microalbumin (mg/L), creatinine (mg/dL), Na (mmol/L), K (mmol/L), Cl (mmol/L), P (mg/dL), N-acetyl glucosamine (NAG) (U/L) and pH values were measured. Then urea (mg/dL), creatinine (mg/dL), total protein (g/dL) and cystatin (mg/L) values were measured for the blood samples obtained from tail veins. Results The median NAG value for the control group (52.050 U/L) was similar to value for Group II (56.400 U/L), which received gentamicin and the garlic diet. However, the median NAG value for Group I (77.030 U/L), which received gentamicin without garlic supplementation, was determined to be statistically significantly higher (p = 0.010) than the value for the control group. In addition, the mean cystatin value for Group II (1.360 U/L) was found to be statistically significantly lower than the value for the Group I (2.240 U/L) (p = 0.015). Conclusions In this study we showed the effect of 4% pulverized garlic supplemented diet for preventing nephrotoxicity induced by gentamicin in rats by using as parameters NAG in urine samples and cystatin C in serum samples. .
Descritores: Antibacterianos/toxicidade
Suplementos Nutricionais
Alho
Gentamicinas/toxicidade
Rim/efeitos dos fármacos
-Albuminúria
Acetilglucosamina/urina
Creatinina/sangue
Creatinina/urina
Cistatina C/sangue
Distribuição Aleatória
Ratos Wistar
Valores de Referência
Reprodutibilidade dos Testes
Resultado do Tratamento
Urinálise
Ureia/sangue
Limites: Animais
Masculino
Responsável: BR1.1 - BIREME


  3 / 11 LILACS  
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Id: lil-722174
Autor: Lima, V.V.; Lobato, N.S.; Filgueira, F.P.; Webb, R.C.; Tostes, R.C.; Giachini, F.R..
Título: Vascular O-GlcNAcylation augments reactivity to constrictor stimuli by prolonging phosphorylated levels of the myosin light chain
Fonte: Braz. j. med. biol. res = Rev. bras. pesqui. méd. biol;47(10):826-833, 10/2014. graf.
Idioma: en.
Projeto: FAPESP; . Fundação de Amparo è Pesquisa do Estado de Mato Grosso; . CAPES and CNPq.
Resumo: O-GlcNAcylation is a modification that alters the function of numerous proteins. We hypothesized that augmented O-GlcNAcylation levels enhance myosin light chain kinase (MLCK) and reduce myosin light chain phosphatase (MLCP) activity, leading to increased vascular contractile responsiveness. The vascular responses were measured by isometric force displacement. Thoracic aorta and vascular smooth muscle cells (VSMCs) from rats were incubated with vehicle or with PugNAc, which increases O-GlcNAcylation. In addition, we determined whether proteins that play an important role in the regulation of MLCK and MLCP activity are directly affected by O-GlcNAcylation. PugNAc enhanced phenylephrine (PE) responses in rat aortas (maximal effect, 14.2±2 vs 7.9±1 mN for vehicle, n=7). Treatment with an MLCP inhibitor (calyculin A) augmented vascular responses to PE (13.4±2 mN) and abolished the differences in PE-response between the groups. The effect of PugNAc was not observed when vessels were preincubated with ML-9, an MLCK inhibitor (7.3±2 vs 7.5±2 mN for vehicle, n=5). Furthermore, our data showed that differences in the PE-induced contractile response between the groups were abolished by the activator of AMP-activated protein kinase (AICAR; 6.1±2 vs 7.4±2 mN for vehicle, n=5). PugNAc increased phosphorylation of myosin phosphatase target subunit 1 (MYPT-1) and protein kinase C-potentiated inhibitor protein of 17 kDa (CPI-17), which are involved in RhoA/Rho-kinase-mediated inhibition of myosin phosphatase activity. PugNAc incubation produced a time-dependent increase in vascular phosphorylation of myosin light chain and decreased phosphorylation levels of AMP-activated protein kinase, which decreased the affinity of MLCK for Ca2+/calmodulin. Our data suggest that proteins that play an important role in the regulation of MLCK and MLCP activity are directly affected by O-GlcNAcylation, favoring vascular contraction.
Descritores: Músculo Liso Vascular/fisiologia
Cadeias Leves de Miosina/metabolismo
Processamento de Proteína Pós-Traducional/fisiologia
Vasoconstrição/fisiologia
-Aorta Torácica
Acetilglucosamina/análogos & derivados
Acetilglucosamina/farmacologia
Acilação/efeitos dos fármacos
Acilação/fisiologia
Aminoimidazol Carboxamida/análogos & derivados
Aminoimidazol Carboxamida/farmacologia
Azepinas/farmacologia
Western Blotting
Inibidores Enzimáticos/farmacologia
Hipoglicemiantes/farmacologia
Músculo Liso Vascular/efeitos dos fármacos
Músculo Liso Vascular/metabolismo
Quinase de Cadeia Leve de Miosina/metabolismo
Fosfatase de Miosina-de-Cadeia-Leve/metabolismo
Oxazóis/farmacologia
Oximas/farmacologia
Fenilcarbamatos/farmacologia
Fenilefrina/agonistas
Fosforilação/efeitos dos fármacos
Fosforilação/fisiologia
Ratos Wistar
Ribonucleotídeos/farmacologia
Vasoconstrição/efeitos dos fármacos
Vasoconstritores/farmacologia
beta-N-Acetil-Hexosaminidases/antagonistas & inibidores
Limites: Animais
Masculino
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


  4 / 11 LILACS  
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Coelho, Paulo Marcos Zech
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Id: lil-606653
Autor: Martins-Souza, Raquel Lopes; Pereira, Cintia Aparecida Jesus; Rodrigues, Leonardo; Araújo, Emília Souza; Coelho, Paulo Marcos Zech; Corrêa Júnior, Ary; Negrão-Corrêa, Deborah.
Título: Participation of N-acetyl-D-glucosamine carbohydrate moieties in the recognition of Schistosoma mansoni sporocysts by haemocytes of Biomphalaria tenagophila
Fonte: Mem. Inst. Oswaldo Cruz;106(7):884-891, Nov. 2011. ilus, graf.
Idioma: en.
Resumo: Lectin-carbohydrate binding may be involved in the recognition of Schistosoma mansoni sporocysts by haemocytes of Biomphalaria; therefore, we tested if this interaction is associated with snail resistance against Schistosoma infection. In vitro data showed that most of the S. mansoni sporocysts cultured with haemocytes from Biomphalaria glabrata BH, a highly susceptible snail strain, had a low number of cells that adhered to their tegument and a low mortality rate. Moreover, the addition of N-acetyl-D-glucosamine (GlcNAc) did not alter this pattern of adherence and mortality. Using haemocytes and haemolymph of Biomphalaria tenagophila Cabo Frio, we observed a high percentage of sporocysts with adherent cells, but complete encapsulation was not detected. Low concentrations of GlcNAc increased haemocyte binding to the sporocysts and mortality, which returned to basal levels with high concentrations of the carbohydrate. In contrast, haemocytes plus haemolymph from B. tenagophila Taim encapsulated cellular adhesion index of level 3 and destroyed over 30 percent of the S. mansoni sporocysts in culture. Interestingly, the addition of GlcNAc, but not mannose, to the culture medium resulted in the significant inhibition of cellular adhesion to the parasite tegument and the reduction of parasite mortality, suggesting that GlcNAc carbohydrate moieties are important to the recognition of S. mansoni by B. tenagophila Taim.
Descritores: Acetilglucosamina/imunologia
Biomphalaria/parasitologia
Hemócitos/parasitologia
Hemolinfa/parasitologia
Oocistos/fisiologia
Schistosoma mansoni/imunologia
-Biomphalaria/citologia
Carboidratos/imunologia
Interações Hospedeiro-Parasita
Limites: Animais
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


  5 / 11 LILACS  
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Previato, José O
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Id: lil-520901
Autor: Todeschini, Adriane R; Almeida, Eliane G de; Agrellos, Orlando A; Jones, Christopher; Previato, José O; Mendonça-Previato, Lucia.
Título: á-N-acetylglucosamine-linked O-glycans of sialoglycoproteins (Tc-mucins) from Trypanosoma cruzi Colombiana strain
Fonte: Mem. Inst. Oswaldo Cruz;104(supl.1):270-274, July 2009. ilus, graf.
Idioma: en.
Resumo: Trypanosoma cruzi sialoglycoproteins (Tc-mucins) are mucin-like molecules linked to a parasite membrane via a glycosylphosphatidylinositol anchor. We previously determined the structures of Tc-mucin O-glycan domains from several T. cruzi strains and observed significant differences among them. We now report the amino acid content and structure of Tc-mucin O-glycan chains from T. cruzi Colombiana, a strain resistant to common trypanocidal drugs. Amino acid analysis demonstrated the predominance of threonine residues (42%) and helped to identify the O-glycans as belonging to a Tc-mucin family that contain a ²-galactofuranose (²-Galf) residue attached to an á-N-acetylglucosamine (á-GlcNAc) O-4, with the most complex glycan, a pentasaccharide-GlcNAc-ol with a branched trigalactopyranose chain, on the GlcNAc O-6. The presence of ²-Galf on O-glycans from T. cruzi Colombiana mucins supports the use of glycosylation as a phylogenetic marker for the classification of Colombiana in the T. cruzi I group.
Descritores: Acetilglucosamina/análise
Configuração de Carboidratos
Mucinas/química
Oligossacarídeos/análise
Sialoglicoproteínas/análise
Trypanosoma cruzi/química
-Cromatografia Líquida de Alta Pressão
Eletroforese em Gel de Poliacrilamida
Espectroscopia de Ressonância Magnética
Trypanosoma cruzi/classificação
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


  6 / 11 LILACS  
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Texto completo SciELO Chile
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Id: lil-454063
Autor: Fuenzalida, M; Lemus, S; Illanes, J; Montiel, E; Acuña, O; Lemus, D.
Título: Histochemical detection of sugar residues in lizard teeth (Liolaemus gravenhorsti): a lectin-binding study
Fonte: Biol. Res;33(3/4):215-226, 2000. ilus.
Idioma: en.
Resumo: The structural diversity of the many oligosaccharide chains of surface glycoconjugates renders them likely candidates for modulators of cell-interactions, cellular movements, differentiation, and cellular recognition. A selection of different lectins was used to investigate the appearance of cellular distribution and changes in sugar residues during tooth development in the polyphyodont lizard, Liolaemus gravenhorsti. Lectins from three groups were used: (1) N-acetylgalactosamine specificity: BS-1, PNA, RCA-120; (2) N-acetylglucosamine specificity: ECA; and (3) fucose specificity: UEA 1 and LTA.. Digital images were processed using Scion Image. Grayscale graphics in each image were obtained. The lectins used showed a strong, wide distribution of the L-fucose and N-acetylgalactosamine at the cell surface and in the cytoplasm of multinucleate odontoclast cell, while mononuclear odontoclast cells showed no binding, suggesting some roles that the residues sugar might play in the resorption of dentine or with multinucleation of odontoclast after the attachment to the dentine surface in this polyphyodont species. Further studies must be planned to determine the specific identities of these glycoconjugates,and to elucidate the roles played by these sugar residues in the complex processes related to odontogenesis in polyphyodont species.
Descritores: Acetilgalactosamina/análise
Acetilglucosamina/análise
Dente/química
Fucose/análise
Lectinas
Lagartos
Osteoclastos/química
-Dente/citologia
Histocitoquímica
Odontogênese
Limites: Animais
Responsável: BR1.1 - BIREME


  7 / 11 LILACS  
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Id: lil-396047
Autor: Ferreira-Medeiros, Mildred; Correa-Gillieron, Elenice.
Título: Recognition of n-acetylglucosamine (GLyNAc) and poly-i-acetyllactosamine residues in vessels of the rat pineal gland
Fonte: Int. j. morphol;22(4):285-290, dez. 2004. ilus.
Idioma: en.
Resumo: Las lectinas son proteínas que contienen áreas singulares para el reconocimiento de secuencias de azúcares en los glicoconjugados. La lecitina del tomate Lycopersicon esculentum (LEL) es capaz de reconocer específicamente los residuos de N-acetil-glucosamina (Gly-Nac) y poli-N-acetil-lactosamina. Utilizamos la técnica histoquímica para LEL conjugada a la biotina con el propósito de investigar en la glándula pineal de ratones adultos y durante el desarrollo, las estructuras morfológicas capaces de unirse a esta lecitina. Nuestros resultados experimentales mostraron un material de coloración por la LEL, solamente en la superficie de las células endoteliales de todos los vasos sanguíneos y en todas las regiones de la glándula. La excepción ocurrió en los ratones con un día pos-natal (PN1), donde solamente los vasos de la región más periférica de la glándula presentaban coloración marrón amarillenta por la LEL, pero ninguno presentaba esta coloración en la región más central de la glándula. La reacción apareció especialmente en el espacio interno de las pseudo-rosetas, demostrando así que este espacio está, seguramente, representado por un vaso.
Descritores: Glândula Pineal/enzimologia
Glândula Pineal/irrigação sanguínea
Lycopersicon esculentum/enzimologia
Lycopersicon esculentum/química
-Animais Recém-Nascidos
Acetilglucosamina/isolamento & purificação
Acetilglucosamina/análise
Células Endoteliais
Células Endoteliais/enzimologia
Fosfatidilcolinas
Glicoconjugados/análise
N-Acetil-Lactosamina Sintase
Ratos Wistar
Limites: Animais
Responsável: CL1.1 - Biblioteca Central


  8 / 11 LILACS  
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Id: lil-384246
Autor: Rose, T. L; Gomes, V. M; Cunha, M. da; Fernandes, K. V; Xavier-Filho, J.
Título: Effect of sugars on the association between cowpea vicilin (7S storage proteins) and fungal cells
Fonte: Biocell;27(2):173-179, Aug. 2003.
Idioma: en.
Resumo: Vicilins (7S storage proteins) found in various legume seeds have been previously shown to interfere with the germination of spores or conidia of phytopathogenic fungi and inhibit yeast growth and glucose stimulated acidification of the medium by yeast cells. In the present work vicilins from cowpea (Vigna unguiculata) seeds were added to the growth medium of Saccharomyces cerevisiae cells and Fusarium oxysporum conidia. Helix pomatia lectin, wheat germ agglutinin and Ulex europaeus lectin were used to identify differences in the binding of the vicilins to the surface of cells of S. cerevisiae and F. oxysporum treated with this protein. After the growth period, the material in suspension (yeast cells) was centrifuged and the final pellet was also treated with different sugar (glucose, sucrose, glucosamine, N-acetyl-glucosamine) concentrations and 0.1 M HCl for extraction of vicilins associated to chitinous structures present in yeast cells. Our results showed that vicilin sub-units were present in the different sugar extracts of yeast cells pretreated with the vicilins and these proteins were eluted by 0.5 M solutions of sugars in the following order of efficiency of elution: N-acetyl-glucosamine, sucrose/glucose and glucosamine.
Descritores: Carboidratos/farmacologia
Ligação Competitiva/efeitos dos fármacos
Membrana Celular/efeitos dos fármacos
Parede Celular/efeitos dos fármacos
Proteínas de Plantas/farmacologia
-Acetilglucosamina/farmacologia
Fungos/efeitos dos fármacos
Fungos/crescimento & desenvolvimento
Fungos/ultraestrutura
Fusarium/efeitos dos fármacos
Fusarium/crescimento & desenvolvimento
Fusarium/ultraestrutura
Glucosamina/farmacologia
Glucose/farmacologia
Ligação Competitiva/fisiologia
Microscopia Eletrônica
Membrana Celular/metabolismo
Membrana Celular/ultraestrutura
Parede Celular/metabolismo
Parede Celular/ultraestrutura
Sacarose/farmacologia
Saccharomyces cerevisiae/efeitos dos fármacos
Saccharomyces cerevisiae/crescimento & desenvolvimento
Saccharomyces cerevisiae/ultraestrutura
Sítios de Ligação/efeitos dos fármacos
Sítios de Ligação/fisiologia
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


  9 / 11 LILACS  
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Texto completo SciELO Brasil
Brandelli, A
Texto completo
Id: lil-226214
Autor: Passos, E. P; Brugnara, L; Facin, A. C; Riffel, A; Lima, G. R; Freitas, V; Brandelli, A.
Título: Peritoneal fluid modulates the sperm acrosomal exocytosis induced by N-acetylglucosaminyl neoglycoprotein
Fonte: Braz. j. med. biol. res = Rev. bras. pesqui. méd. biol;32(1):59-65, Jan. 1999. graf.
Idioma: en.
Resumo: The effect of peritoneal fluid (PF) on the human sperm acrosome reaction (AR) was tested. Sperm was pre-incubated with PF and the AR was induced by calcium ionophore A23187 and a neoglycoprotein bearing N-acetylglycosamine residues (NGP). The AR induced by calcium ionophore was inhibited 40 percent by PF from controls (PFc) and 50 percent by PF from the endometriosis (PFe) group, but not by PF from infertile patients without endometriosis (PFi). No significant differences were found in the spontaneous AR. When the AR was induced by NGP, pre-incubation with PFc reduced (60 percent) the percentage of AR, while PFe and PFi caused no significant differences. The average rates of acrosome reactions obtained in control, NGP- and ionophore-treated sperm showed that NGP-induced exocytosis differed significantly between the PFc (11 percent) and PFe/PFi groups (17 percent), and the ionophore-induced AR was higher for PFi (33 percent) than PFc/PFe (25 percent). The incidence of the NGP-induced AR was reduced in the first hour of pre-incubation with PFc and remained nearly constant throughout 4 h of incubation. The present data indicate that PF possesses a protective factor which prevents premature AR
Descritores: Acetilglucosamina/farmacologia
Acrossomo/efeitos dos fármacos
Líquido Ascítico
Exocitose/efeitos dos fármacos
Ionóforos/farmacologia
-Endometriose
Capacitação Espermática/efeitos dos fármacos
Limites: Feminino
Humanos
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


  10 / 11 LILACS  
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Souza, Wanderley de
Id: lil-102118
Autor: Degett, Jens; Souto Padron, Thais; Souza, Wanderley de.
Título: Localization of sugar-binding proteins in Trypanosoma cruzi using gold-labeled neoglycoproteins
Fonte: Microsc. electron. biol. celular;14(1):11-8, 1990. ilus.
Idioma: en.
Resumo: Neoglicoproteínas marcadas com ouro coloidal foram utilizadas para localizaçäo de sítios de ligaçäo de carboidratos em Trypanosoma cruzi. Sítios de ligaçäo para N-acetil-D-glicosamina e D-galactose foram observadas na superfície de cerca de 80 e 5 a 10% das formas tripomastigotas, respectivamente. Sítios de ligaçäo para D-manose näo foram observados na superfície de tripomastigotas. Neoglicoproteínas que reconhecem N-acetil-D-glicosamina, D-galactose e D-manose näo se ligam á superficie das formas epimastigota e amastigota. Marcaçäo de núcleo e cinetoplasto foi observada com a neoglicoproteína que reconhece N-acetil-D-glicosamina. Os resultados obtidos säo discutidos levando em consideraçäo o papel que proteínas que se ligam a carboidratos podem desempenhar no processo de interaçäo entre T. cruzi e a célula hospedeira
Descritores: Carboidratos/metabolismo
Glicoproteínas/análise
Ouro
Trypanosoma cruzi/análise
-Acetilglucosamina/metabolismo
Sítios de Ligação
Galactose/metabolismo
Microscopia Eletrônica
Trypanosoma cruzi/crescimento & desenvolvimento
Trypanosoma cruzi/ultraestrutura
Limites: Animais
Responsável: BR1.1 - BIREME



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