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Id: biblio-897789
Autor: Zhang, Lei; Zhou, Xian-Jin; Zhan, Li-Ying; Wu, Xiao-Jing; Li, Wen-Lan; Zhao, Bo; Meng, Qing-Tao; Xia, Zhong-Yuan.
Título: Dexmedetomidine preconditioning protects against lipopolysaccharides-induced injury in the human alveolar epithelial cells / Pré-condicionamento com dexmedetomidina protege contra lesões induzidas por lipopolissacarídeos em células epiteliais alveolares humanas
Fonte: Rev. bras. anestesiol;67(6):600-606, Nov.-Dec. 2017. graf.
Idioma: en.
Resumo: Abstract Background and objectives Dexmedetomidine (DEX) has demonstrated the preconditioning effect and shown protective effects against organize injury. In this study, using A549 (human alveolar epithelial cell) cell lines, we investigated whether DEX preconditioning protected against acute lung injury (ALI) in vitro. Methods A549 were randomly divided into four groups (n = 5): control group, DEX group, lipopolysaccharides (LPS) group, and D-LPS (DEX + LPS) group. Phosphate buffer saline (PBS) or DEX were administered. After 2 h preconditioning, the medium was refreshed and the cells were challenged with LPS for 24 h on the LPS and D-LPS group. Then the malondialdehyde (MDA), superoxide dismutase (SOD), Bcl-2, Bax, caspase-3 and the cytochrome c in the A549 were tested. The apoptosis was also evaluated in the cells. Results Compare with LPS group, DEX preconditioning reduced the apoptosis (26.43% ± 1.05% vs. 33.58% ± 1.16%, p < 0.05) in the A549, which is correlated with decreased MDA (12.84 ± 1.05 vs. 19.16 ± 1.89 nmoL.mg-1 protein, p < 0.05) and increased SOD activity (30.28 ± 2.38 vs. 20.86 ± 2.19 U.mg-1 protein, p < 0.05). DEX preconditioning also increased the Bcl-2 level (0.53 ± 0.03 vs. 0.32 ± 0.04, p < 0.05) and decreased the level of Bax (0.49 ± 0.04 vs. 0.65 ± 0.04, p < 0.05), caspase-3 (0.54 ± 0.04 vs. 0.76 ± 0.04, p < 0.05) and cytochrome c. Conclusion DEX preconditioning has a protective effect against ALI in vitro. The potential mechanisms involved are the inhibition of cell death and improvement of antioxidation.

Resumo Justificativa e objetivos Dexmedetomidina (DEX) demonstrou ter efeito pré-condicionante e também efeitos protetores contra lesão organizada. Neste estudo, com células A549 (células epiteliais alveolares humanas), investigamos se o pré-condicionamento com DEX proporcionaria proteção contra lesão pulmonar aguda (LPA) in vitro. Métodos Células A549 foram aleatoriamente distribuídas em quatro grupos (n = 5): controle, DEX, lipopolissacarídeos (LPS) e D-LPS (DEX + LPS). Administramos solução de PBS (tampão fosfato-alcalino) ou DEX. Após 2 h de pré-condicionamento, o meio foi renovado e as células desafiadas com LPS por 24 h nos grupos LPS e D-LPS. Em seguida, malondialdeído (MDA), superóxido dismutase (SOD), Bcl-2, Bax, caspase-3 e em A549 foram testados. Apoptose também foi avaliada nas células. Resultados Em comparação com o grupo LPS, o pré-condicionamento com DEX reduziu a apoptose (26,43% ± 1,05% vs. 33,58% ± 1,16%, p < 0,05) em células A549, o que está correlacionado com a diminuição de MDA (12,84 ± 1,05 vs. 19,16 ± 1,89 nmol.mg-1 de proteína, p < 0,05) e aumento da atividade de SOD (30,28 ± 2,38 vs. 20,86 ± 2,19 U.mg-1 de proteína, p < 0,05). O pré-condicionamento com DEX também aumentou o nível de Bcl-2 (0,53 ± 0,03 vs. 0,32 ± 0,04, p < 0,05) e diminuiu o nível de Bax (0,49 ± 0,04 vs. 0,65 ± 0,04, p < 0,05), caspase-3 (0,54 ± 0,04 vs. 0,76 ± 0,04, p < 0,05) e citocromo c. Conclusão O pré-condicionamento com DEX tem efeito protetor contra LPA in vitro. Os potenciais mecanismos envolvidos são inibição da morte celular e melhoria da antioxidação.
Descritores: Lipopolissacarídeos/efeitos adversos
Dexmedetomidina/farmacologia
Células Epiteliais Alveolares/efeitos dos fármacos
Hipnóticos e Sedativos/farmacologia
-Distribuição Aleatória
Células Cultivadas
Lipopolissacarídeos/antagonistas & inibidores
Limites: Humanos
Responsável: BR1.1 - BIREME


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Id: biblio-838758
Autor: Göncü, Tuğba; Oğuz, Elif; Sezen, Hatice; Koçarslan, Sezen; Oğuz, Halit; Akal, Ali; Adıbelli, Fatih Mehmet; Çakmak, Sevim; Aksoy, Nurten.
Título: Anti-inflammatory effect of lycopene on endotoxin-induced uveitis in rats / Efeito anti-inflamatório do licopeno na uveíte induzida por endotoxina em ratos
Fonte: Arq. bras. oftalmol;79(6):357-362, Nov.-Dec. 2016. graf.
Idioma: en.
Projeto: Harran University Scientific Research Council.
Resumo: ABSTRACT Purpose: We evaluated the efficacy of lycopene, a dietary carotenoid and potent antioxidant, against ocular inflammation and oxidative stress in an experimental uveitis model. Methods: Endotoxin-induced uveitis (EIU) was induced in Sprague-Dawley rats by a single subcutaneous injection of 200 μg lipopolysaccharide (LPS). Induction of EIU was preceded by daily intraperitoneal injection of 10 mg/kg lycopene for three consecutive days (Lycopene + LPS group) or equivolume vehicle (Vehicle + LPS group). A positive control group received 1 mg/kg dexamethasone pretreatment (DEX + LPS), and a negative control group received daily vehicle injection but no LPS (Vehicle Control). Twenty-four hours after LPS or final vehicle administration, eyes were enucleated, and aqueous humor was collected for measurement of the number of infiltrating cells, total protein concentration, and levels of nitric oxide (NO), tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), and oxidative stress markers. Inflammatory response severity was compared among groups clinically and histopathologically. Results: Infiltrating cell number, total protein concentration, and NO, TNF-α, and IL-6 levels were significantly elevated in the aqueous humor of Vehicle + LPS group rats compared to Vehicle Controls. Compared to the Vehicle + LPS group, lycopene pretreatment significantly reduced aqueous humor concentrations of oxidative stress markers, NO (0.29 ± 0.1 μM vs. 0.19 ± 0.1 μM, p=0.003), TNF-α (71.0 ± 22.3 ng/ml vs. 50.1 ± 2.1 ng/ml, p=0.043), and IL-6 (121.6 ± 3.0 pg/ml vs. 111.1 ± 5.6 pg/ml, p=0.008). Inflammatory score was also reduced (2.0 ± 0.0 vs. 0.4 ± 0.5, p=0.001). Lycopene reduced the infiltrating cell count and protein concentration, but differences did not reach significance. Most lycopene effects were equivalent to dexamethasone. Conclusions: Lycopene may aid in the clinical management of uveitis by suppressing inflammation and oxidative stress.

RESUMO Objetivo: Avaliamos o efeito do licopeno, um carotenóide dietético e um potente anti-oxidante, sobre a inflamação ocular e estresse oxidativo em modelo de uveíte experimental. Métodos: Uveíte foi induzida por endotoxina (EIU) em ratos Sprague-Dawley por uma única injeção subcutânea de 200 ug de lipopolissacárido (LPS). A indução de EIU foi precedida por injeção intraperitoneal de licopeno em uma dose de 10 mg/kg (grupo LPS + Licopeno) ou veículo de mesmo volume (grupo LPS + Veículo), durante 3 dias consecutivos. O grupo controle positivo recebeu uma dose de 1 mg/kg de Dexametasona (grupo DEX + LPS) e o grupo controle negativo recebeu doses diárias de veículo mas sem LPS (grupo Controle Veículo). Vinte e quatro horas após a administração do LPS, os olhos foram enucleados, humor aquoso foi recolhido, e o número de células infiltrativas, a concentração de proteína, assim como os níveis de óxido nítrico (NO), fator de necrose tumoral α (TNF-α), interleucina-6 e marcadores de estresse oxidativo foram determinados no humor aquoso. Além disso, a resposta inflamatória foi avaliada clinicamente e histologicamente. Resultados: As células infiltrativas, concentração de proteína, o NO, TNF-α, interleucina-6 foram significativamente elevados no humor aquoso de ratos do grupo Grupo LPS + Veículo quando comparados ao Grupo Controle Veículo. O tratamento com licopeno diminuiu significativamente estes aumentos. Comparado ao Grupo LPS + Veículo, o licopeno reduziu significativamente as concentrações no humor aquoso dos marcadores de estresse oxidativo e NO (de 0,29 ± 0,1 μM para 0,19 ± 0,1 μM, p=0,003), o TNF-α (de 71,0 ± 22,3 ng/ml para 50,1 ± 2,1 ng/ml, p=0,043), interleucina-6 (de 121,6 ± 3,0 pg/ml para 111,1 ± 5,6 pg/ml, p=0,008). Do mesmo modo, o aumento do número de células infiltrativas no tecido uveal em seções histológicas foi significativamente inibido pelo licopeno, a pontuação inflamatória diminuiu de 2,0 ± 0,0 para 0,4 ± 0,5, p=0,001. Embora, não tenha sido estatisticamente significativo, o licopeno reduziu a contagem de células infiltrativas e a concentração de proteínas no humor aquoso. Conclusões: Estes resultados sugerem que o licopeno pode ter efeitos benéficos no tratamento da inflamação ocular, através dos seus efeitos anti-inflamatórios e antioxidantes.
Descritores: Uveíte/tratamento farmacológico
Carotenoides/uso terapêutico
Anti-Inflamatórios/uso terapêutico
Antioxidantes/uso terapêutico
-Humor Aquoso/metabolismo
Uveíte/induzido quimicamente
Uveíte/patologia
Lipopolissacarídeos
Interleucina-6/metabolismo
Fator de Necrose Tumoral alfa/metabolismo
Ratos Sprague-Dawley
Estresse Oxidativo
Modelos Animais de Doenças
Olho/patologia
Licopeno
Óxido Nítrico/metabolismo
Limites: Animais
Ratos
Responsável: BR1.1 - BIREME


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Id: biblio-1101254
Autor: FIGUEIREDO, Rebeca Dantas Alves; ORTEGA, Adriana Cabrera; GONZÁLEZ MALDONADO, Laura Andrea; CASTRO, Ricardo Dias de; ÁVILA-CAMPOS, Mario Julio; ROSSA JUNIOR, Carlos; AQUINO, Sabrina Garcia de.
Título: Perillyl alcohol has antibacterial effects and reduces ROS production in macrophages
Fonte: J. appl. oral sci;28:e20190519, 2020. tab, graf.
Idioma: en.
Resumo: Abstract Natural products have emerged as a rich source of bioactive compounds for adjunctive treatments of many infectious and inflammatory conditions, including periodontitis. Among the monoterpenes with significant biological properties, there is the perillyl alcohol (POH), which can be found in several essential oils and has shown immunomodulatory properties in recent studies, which may be interesting in the treatment of non-neoplastic inflammatory disorders. Objective To determine the antibacterial and immune modulatory activities of the POH. Methodology The minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC) of the POH for two significant Gram-negative periodontal pathogens were determined by macrodilution and subculture, respectively. Cell proliferation and cytotoxicity in RAW 264.7 macrophages were determined by Trypan Blue and mitochondrial enzymatic activity assay. The modulation of reactive oxygen species (ROS) was analyzed by flow cytometry and expression of TNF and arginase-1 by real-time PCR. Results The POH was effective against P. gingivalis (ATCC 33277) and F. nucleatum (ATCC 25586) with MIC= MBC=1600 μM. No cytotoxicity up to 100 µM was observed on macrophages. The cell proliferation was inhibited from 48 hours at 100 μM (p<0.05) and 250 μM (p<0.01). The POH increased ROS production at both 10 μM and 100 μM (p<0.05) in unstimulated cells. The PMA-induced ROS production was not affected by POH, whereas 100 μM significantly reduced lipopolysaccharide-induced (LPS-induced) ROS. The expression of TNF was not affected by POH in unstimulated cells or in cells polarized to M1 phenotype, whereas both concentrations of POH reduced (p<0.05) the expression of arginase-1 in M2-polarized macrophages. Conclusion The POH has antibacterial activity against periodontal pathogens and reduced proliferation of murine macrophages without significant cytotoxicity at concentrations up to 100 μM. In addition, the POH reduced the LPS-induced ROS and the expression of arginase-1 in M2-polarized macrophages.
Descritores: Fusobacterium nucleatum/efeitos dos fármacos
Espécies Reativas de Oxigênio/análise
Porphyromonas/efeitos dos fármacos
Monoterpenos/farmacologia
Macrófagos/efeitos dos fármacos
Antibacterianos/farmacologia
-Arginase/análise
Fatores de Tempo
Produtos Biológicos/farmacologia
Testes de Sensibilidade Microbiana
Expressão Gênica
Lipopolissacarídeos/farmacologia
Reprodutibilidade dos Testes
Fator de Necrose Tumoral alfa/análise
Fusobacterium nucleatum/crescimento & desenvolvimento
Espécies Reativas de Oxigênio/metabolismo
Porphyromonas/crescimento & desenvolvimento
Proliferação de Células/efeitos dos fármacos
Reação em Cadeia da Polimerase em Tempo Real
Citometria de Fluxo
Células RAW 264.7
Macrófagos/metabolismo
Limites: Animais
Camundongos
Responsável: BR28.1 - Serviço de Biblioteca e Documentação Professor Doutor Antônio Gabriel Atta


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Id: biblio-1007454
Autor: Mena, Licet; Sierra, Roxana; Valle, Maikel; Molina, Vivian; Rodriguez, Sandra; Merino, Nelson; Zamora, Zullyt; González, Victor; Medina, Jose Alberto.
Título: Acrocomia crispa fruits lipid extract prevents LPS-induced acute lung injury in mice / Extracto lipídico de los frutos de Acrocomia crispa previene el daño pulmonar agudo inducido por LPS en ratones
Fonte: Bol. latinoam. Caribe plantas med. aromát;18(1):16-26, ene. 2019. ilus, tab.
Idioma: en.
Resumo: The aim of this study was to evaluate the effects of single oral doses of D-005 (a lipid extract obtained from the fruit oil of Acrocomia crispa) on LPS-induced acute lung injury (ALI) in mice. D-005 batch composition was: lauric (35.8%), oleic (28.4%), myristic (14.2%), palmitic (8.9%), stearic (3.3%), capric (1.9%), caprylic (1.2%), and palmitoleic (0.05%) acids, for a total content of fatty acids of 93.7%. D-005 (200 mg/kg) significantly reduced lung edema (LE) (≈ 28% inhibition) and Lung Weight/Body Weight ratio (LW/BW) (75.8% inhibition). D-005 (25, 50, 100 and 200 mg/kg) produced a significant reduction of Histological score (59.9, 56.1, 53.5 and 73.3% inhibition, respectively). Dexamethasone, as the reference drug, was effective in this experimental model. In conclusion, pretreatment with single oral doses of D-005 significantly prevented the LPS-induced ALI in mice.

El objetivo de este estudio fue evaluar los efectos de dosis orales únicas de D-005 (extracto lipídico obtenido del aceite de frutos de Acrocomia crispa) sobre el daño pulmonar agudo (DPA) inducido por LPS en ratones. La composición del lote de D-005 fue: ácido láurico (35.8%), oleico (28.4%), mirístico (14.2%), palmítico (8.9%), esteárico (3.3%), cáprico (1.9%), caprílico (1.2%) y palmitoleico (0.05%), con un contenido total de ácidos grasos de 93.7%. D-005 (200 mg/kg) redujo significativamente el edema pulmonar (EP) (≈ 28% de inhibición) y la relación peso pulmón/peso corporal (PP/PC) (75.8% de inhibición). D-005 (25, 50, 100 y 200 mg/kg) produjo una reducción significativa de la puntuación histológica (59.9, 56.1, 53.5 y 73.3% de inhibición, respectivamente). La dexametasona, fármaco de referencia, fue efectiva en este modelo experimental. En conclusión, el pretratamiento con dosis orales únicas de D-005 previno significativamente el DPA inducido por LPS en ratones.
Descritores: Extratos Vegetais/administração & dosagem
Arecaceae
Lesão Pulmonar Aguda/prevenção & controle
-Extratos Vegetais/química
Lipopolissacarídeos/efeitos adversos
Administração Oral
Cromatografia Gasosa
Lesão Pulmonar Aguda/induzido quimicamente
Ácidos Graxos/análise
Frutas
Pulmão/efeitos dos fármacos
Limites: Animais
Camundongos
Responsável: CL1.1 - Biblioteca Central


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Id: biblio-887604
Autor: Bonfante, Ivan Luiz Padilha; Chacon-Mikahil, Mara Patricia Traina; Brunelli, Diego Trevisan; Gáspari, Arthur Fernandes; Duft, Renata Garbellini; Oliveira, Alexandre Gabarra; Araujo, Tiago Gomes; Saad, Mario Jose Abdalla; Cavaglieri, Cláudia Regina.
Título: Obese with higher FNDC5/Irisin levels have a better metabolic profile, lower lipopolysaccharide levels and type 2 diabetes risk
Fonte: Arch. endocrinol. metab. (Online);61(6):524-533, Dec. 2017. tab, graf.
Idioma: en.
Projeto: Foundation of São Paulo Research (FAPESP).
Resumo: ABSTRACT Objective: Thus, the aim of this study was to compare if higher or smaller fibronectin type 3 domain-containing protein 5 (FNDC5)/irisin levels are associated with inflammatory and metabolic markers, caloric/macronutrient intake, physical fitness and type 2 diabetes mellitus (T2DM) risk in obese middle-aged men, and also to correlate all variables analyzed with FNDC5/irisin. Subjects and methods: On the basis of a cluster study, middle-aged obese men (IMC: 31.01 ± 1.64 kg/m2) were divided into groups of higher and smaller levels of FNDC5/irisin. The levels of leptin, resistin, adiponectin, tumor necrosis factor alpha (TNFα), interleukin 6 and 10 (IL6, IL10), lipopolysaccharide (LPS), glucose, insulin, glycated hemoglobin, insulin resistance and sensibility, lipid profile, risk of T2DM development, body composition, rest energy expenditure, caloric/macronutrient intake and physical fitness were measured. Results: The higher FNDC5/ irisin group presented improved insulin sensibility (homeostasis model assessment - sensibility (HOMA-S) (p = 0.01) and QUICKI index (p < 0.01)), insulin (p = 0.02) and triglyceride levels (p = 0.01), lower insulin resistance (homeostasis model assessment - insulin resistance (HOMA-IR) (p = 0.01), triglycerides/glucose (TYG index) (p = 0.02), neck circumference (p = 0.02), risk of T2DM development (p = 0.02), tendency to decrease serum resistin (p = 0.08) and significant lower LPS levels (p = 0.02). Inverse correlations between FNDC5/irisin and body weight (r −0.46, p = 0.04), neck circumference (r −0.51, p = 0.02), free fat mass (r −0.49, p = 0.02), triglycerides (r −0.43, p = 0.05) and risk of developing T2DM (r −0.61, p = 0.04) were observed. Conclusions: These results suggest that higher FNDC5/irisin levels in obese middle-aged men are related to a better metabolic profile and lower risk of T2DM development and serum LPS, a potential inducer of insulin resistance.
Descritores: Lipopolissacarídeos/sangue
Fibronectinas/sangue
Diabetes Mellitus Tipo 2/etiologia
Obesidade/complicações
-Pressão Sanguínea/fisiologia
Ingestão de Energia/fisiologia
Biomarcadores/sangue
Estudos Transversais
Fatores de Risco
Diabetes Mellitus Tipo 2/fisiopatologia
Diabetes Mellitus Tipo 2/sangue
Teste de Esforço
Aptidão Cardiorrespiratória/fisiologia
Obesidade/fisiopatologia
Obesidade/sangue
Limites: Humanos
Masculino
Adulto
Pessoa de Meia-Idade
Responsável: BR1.1 - BIREME


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Id: biblio-886670
Autor: KWON, DA HYE; JEONG, JIN WOO; CHOI, EUN OK; LEE, HYE WON; LEE, KI WON; KIM, KI YOUNG; KIM, SUNG GOO; HONG, SU HYUN; KIM, GI-YOUNG; PARK, CHEOL; HWANG, HYE-JIN; SON, CHANG-GUE; CHOI, YUNG HYUN.
Título: Inhibitory effects on the production of inflammatory mediators and reactive oxygen species by Mori folium in lipopolysaccharide-stimulated macrophages and zebrafish
Fonte: An. acad. bras. ciênc;89(1,supl):661-674, May. 2017. graf.
Idioma: en.
Projeto: Ministry of Agriculture, Food and Rural Affairs, Republic of Korea.
Resumo: ABSTRACT Mori folium, the leaf of Morus alba L. (Moraceae), has been traditionally used for various medicinal purposes from ancient times to the present. In this study, we examined the effects of water extract of Mori folium (WEMF) on the production of inflammatory mediators, such as nitric oxide (NO) and prostaglandin E2 (PGE2), and reactive oxygen species (ROS) in lipopolysaccharide (LPS)-stimulated murine RAW 264.7 macrophages. Our data indicated that WEMF significantly suppressed the secretion of NO and PGE2 in RAW 264.7 macrophages without any significant cytotoxicity. The protective effects were accompanied by a marked reduction in their regulatory gene expression at the transcription level. WEMF attenuated LPS-induced intracellular ROS production in RAW 264.7 macrophages. It inhibited the nuclear translocation of the nuclear factor-kappa B p65 subunit and the activation of mitogen-activated protein kinases in LPS-treated RAW 264.7 macrophages. Furthermore, WEMF reduced LPS-induced NO production and ROS accumulation in zebrafish. Although more efforts are needed to fully understand the critical role of WEMF in the inhibition of inflammation, the findings of the present study may provide insights into the approaches for Mori folium as a potential therapeutic agent for inflammatory and antioxidant disorders.
Descritores: Peixe-Zebra
Extratos Vegetais/farmacologia
Espécies Reativas de Oxigênio/antagonistas & inibidores
Mediadores da Inflamação/metabolismo
Morus/química
Macrófagos/efeitos dos fármacos
-Prostaglandinas E/metabolismo
Expressão Gênica
Genes Reguladores
Lipopolissacarídeos
Mediadores da Inflamação/antagonistas & inibidores
Células RAW 264.7
Macrófagos/metabolismo
Óxido Nítrico/metabolismo
Limites: Animais
Ratos
Responsável: BR1.1 - BIREME


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Id: biblio-1054909
Autor: CHO, JOSHUA HYONG-JIN; SHU, SARA M; MAHBOD, ARIYA; IRWIN, MICHAEL R.
Título: Lipopolysaccharide-stimulated intracellular cytokines and depressive symptoms in community-dwelling older adults
Fonte: Arch. Clin. Psychiatry (Impr.) = Rev. psiquiatr. clín. (São Paulo) = Psiquiatria clínica;46(5):137-140, Sept.-Oct. 2019. tab, graf.
Idioma: en.
Projeto: National Institutes of Health; . National Institutes of Health; . National Institutes of Health.
Resumo: Abstract Background Inflammation is involved in the pathophysiology of depression, and circulating inflammatory cytokines have been associated with depressive symptoms. However, measuring circulating cytokines have inherent methodological limitations. In vitro lipopolysaccharide (LPS)-stimulated intracellular cytokines (ICCs) overcome these limitations. Furthermore, because psychosocial and physiological stressors activate inflammatory responses and LPS-stimulated ICCs reflect the inflammatory responsivity of monocytes to such stressors, ICCs may reflect individual stress responsivity. Methods This cross-sectional study examined whether LPS-stimulated expression of ICCs in peripheral blood mononuclear cells (PBMCs) is a sensitive inflammation measure correlated with depressive symptoms in 180 community-dwelling older adults. We tested correlations of not only intracellular but also circulating inflammatory markers with depressive symptoms assessed using the 10-item Center for Epidemiological Studies Depression Scale (CES-D). Intracellular markers included expression of interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α), and both in PBMCs. Circulating markers included IL-6, TNF-α, and C-reactive protein (CRP) in plasma. Results None of the correlations were statistically significant. However, in contrast to circulating markers, the correlations of ICCs were consistently in the expected direction, i.e., higher ICC expression correlating with higher depression severity. Discussion Despite the non-significant findings, further research is required for the evaluation of LPS-stimulated ICC expression as biomarkers of depressive symptoms.
Descritores: Lipopolissacarídeos
Citocinas/sangue
Depressão/fisiopatologia
Inflamação/fisiopatologia
-Escalas de Graduação Psiquiátrica
Técnicas In Vitro
Proteína C-Reativa
Monócitos/metabolismo
Biomarcadores/sangue
Estudos Transversais
Interleucina-6/sangue
Fator de Necrose Tumoral alfa/sangue
Depressão/sangue
Inflamação/sangue
Limites: Humanos
Masculino
Feminino
Pessoa de Meia-Idade
Idoso
Idoso de 80 Anos ou mais
Responsável: BR66.1 - Divisão de Biblioteca e Documentação


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Raw, Isaias
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Id: lil-517095
Autor: Higashi, Hisako G; Luna, Expedito; Precioso, Alexander R; Vilela, Marluce; Kubrusly, Flávia S; Dias, Waldely O; Raw, Isaias.
Título: Acellular and "low" pertussis vaccines: adverse events and the role of mutations / Vacinas pertussis acelular e pertussis "low": eventos adversos e o papel das mutações
Fonte: Rev. Inst. Med. Trop. Säo Paulo;51(3):131-134, May-June 2009. ilus.
Idioma: en.
Resumo: Objective: to discuss the current PAHO recommendation that does not support the substitution of traditional cellular DTP vaccine by acellular DTP, and the role of mutations, in humans, as the main cause of rare adverse events, such as epileptic-like convulsions, triggered by pertussis vaccine. Data review: the main components related to toxic effects of cellular pertussis vaccines are the lipopolysaccharide of bacterial cell wall and pertussis toxin. The removal of part of lipopolysaccharide layer has allowed the creation of a safer cellular pertussis vaccine, with costs comparable to the traditional cellular vaccine, and which may be a substitute for the acellular vaccine. Conclusion: The new methodology introduced by Instituto Butantan allows for the development of a new safer pertussis vaccine with low LPS content (Plow), and the use of the lipopolysaccharide obtained in the process in the production of monophosphoryl lipid A. This component has shown potent adjuvant effect when administered together with influenza inactivated vaccine, making possible to reduce the antigen dose, enhancing the production capacity and lowering costs.

Objetivo: Discutir as recomendações da WHO-OPAS que não consideram indicada a substituição da vacina DTP celular clássica pela DTP acelular e o papel de mutações, em humanos, como principal causa dos raros eventos de convulsões epileptiformes desencadeadas pela vacina pertussis. Revisão dos dados: Os principais componentes relacionados aos efeitos tóxicos da vacina pertussis celular são o lipopolissacarídio da parede celular da bactéria e a toxina pertussis. A remoção de parte da camada lipopolissacarídica permitiu a criação de uma vacina pertussis celular, mais segura e de custo comparável ao da vacina celular tradicional, podendo substituir a vacina pertussis acelular. Conclusão: A nova vacina pertussis, com baixo teor de LPS (Plow) desenvolvida pelo Instituto Butantan, além de oferecer uma vacina mais segura, permite o aproveitamento do lipopolissacarídeo para a produção de monofosforil lipídeo A. Esse componente mostrou-se potente como adjuvante e altamente eficiente quando administrado com a vacina de influenza, levando à possibilidade de se reduzir a dose de antígeno, aumentando a capacidade de produção e redução dos custos.
Descritores: Vacina contra Difteria, Tétano e Coqueluche/efeitos adversos
Vacinas contra Difteria, Tétano e Coqueluche Acelular/efeitos adversos
Lipopolissacarídeos/imunologia
Mutação
-Análise Custo-Benefício
Vacina contra Difteria, Tétano e Coqueluche/genética
Vacina contra Difteria, Tétano e Coqueluche/imunologia
Vacinas contra Difteria, Tétano e Coqueluche Acelular/genética
Vacinas contra Difteria, Tétano e Coqueluche Acelular/imunologia
Lipopolissacarídeos/efeitos adversos
Organização Mundial da Saúde
Limites: Humanos
Tipo de Publ: Revisão
Responsável: BR1.1 - BIREME


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Id: biblio-1039115
Autor: Bristot, Giovana; Ascoli, Bruna M; Scotton, Ellen; Géa, Luiza P; Pfaffenseller, Bianca; Kauer-Sant'Anna, Márcia.
Título: Effects of lithium on inflammatory and neurotrophic factors after an immune challenge in a lisdexamfetamine animal model of mania
Fonte: Braz. J. Psychiatry (São Paulo, 1999, Impr.);41(5):419-427, Sept.-Oct. 2019. tab, graf.
Idioma: en.
Resumo: Objective: To evaluate whether an animal model of mania induced by lisdexamfetamine dimesylate (LDX) has an inflammatory profile and whether immune activation by lipopolysaccharides (LPS) has a cumulative effect on subsequent stimuli in this model. We also evaluated the action of lithium (Li) on inflammatory and neurotrophic factors. Methods: Adult male Wistar rats were subjected to an animal model of mania. After the open-field test, they were given LPS to induce systemic immune activation. Subsequently, the animals' blood was collected, and their serum levels of brain-derived neurotrophic factor and inflammatory markers (tumor necrosis factor [TNF]-α, interleukin [IL]-6, IL-1β, IL-10, and inducible nitric oxide synthase [iNOS]) were measured. Results: LDX induced hyperactivity in the animals, but no inflammatory marker levels increased except brain-derived neurotrophic factor (BDNF). Li had no effect on serum BDNF levels but prevented iNOS levels from increasing in animals subjected to immune activation. Conclusion: Although Li prevented an LPS-induced increase in serum iNOS levels, its potential anti-inflammatory effects in this animal model of mania were conflicting.
Descritores: Transtorno Bipolar/imunologia
Modelos Animais de Doenças
Dimesilato de Lisdexanfetamina
Lítio/farmacologia
Anti-Inflamatórios/farmacologia
Fatores de Crescimento Neural/efeitos dos fármacos
-Fatores de Tempo
Transtorno Bipolar/fisiopatologia
Transtorno Bipolar/induzido quimicamente
Ensaio de Imunoadsorção Enzimática
Lipopolissacarídeos/farmacologia
Reprodutibilidade dos Testes
Citocinas/sangue
Resultado do Tratamento
Ratos Wistar
Fator Neurotrófico Derivado do Encéfalo/sangue
Óxido Nítrico Sintase Tipo II/sangue
Locomoção/efeitos dos fármacos
Limites: Animais
Masculino
Tipo de Publ: Estudo de Validação
Responsável: BR1.1 - BIREME


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Id: biblio-1019261
Autor: Yan, Dan; He, Bingshu; Guo, Jie; Li, Shulan; Wang, Jun.
Título: Involvement of TLR4 in the protective effect of intra-articular administration of curcumin on rat experimental osteoarthritis
Fonte: Acta cir. bras;34(6):e201900604, 2019. graf.
Idioma: en.
Projeto: National Natural Science Foundation of China.
Resumo: Abstract Purpose In view of the principal role of Toll-like receptor 4 (TLR4) in mediating sterile inflammatory response contributing to osteoarthritis (OA) pathogenesis, we used lipopolysaccharide (LPS), a known TLR4 activator, to clarify whether modulation of TLR4 contributed to the protective actions of intra-articular administration of curcumin in a classical rat OA model surgically induced by anterior cruciate ligament transection (ACLT). Methods The rats underwent ACLT and received 50μl of curcumin at the concentration of 1 mg mL-1 and 10 μg LPS by intra-articular injection once a week for 8 weeks. Morphological changes of the cartilage and synovial tissues were observed. Apoptotic chondrocytes were detected using TUNEL assay. The concentrations of IL-1β and TNF-ɑ in synovial fluid were determined using ELISA kits. The mRNA and protein expression levels of TLR4 and NF-κB p65 were detected by real-time PCR and Western blotting, respectively. Results Intra-articular administration of curcumin significantly improved articular cartilage injury, suppressed synovial inflammation and down-regulated the overexpression of TLR4 and its downstream NF-κB caused by LPS-induced TLR4 activation in rat osteoarthritic knees. Conclusion The data suggested that the inhibition of TLR4 signal might be an important mechanism underlying a protective effect of local curcumin administration on OA.
Descritores: Osteoartrite/prevenção & controle
Ligamento Cruzado Anterior/cirurgia
Curcumina/farmacologia
-Osteoartrite/induzido quimicamente
Osteoartrite/metabolismo
Ensaio de Imunoadsorção Enzimática
Lipopolissacarídeos
Western Blotting
Reação em Cadeia da Polimerase
Ligamento Cruzado Anterior/patologia
NF-kappa B/metabolismo
Linfotoxina-alfa/metabolismo
Modelos Animais de Doenças
Receptor 4 Toll-Like/efeitos dos fármacos
Receptor 4 Toll-Like/metabolismo
Interleucina-1beta/metabolismo
Injeções Intra-Arteriais
Limites: Animais
Masculino
Ratos
Responsável: BR1.1 - BIREME



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