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Id: biblio-1091471
Autor: Ramírez, Karol; Quesada-Yamasaki, Daniel; Fornaguera-Trías, Jaime.
Título: A Protocol to Perform Systemic Lipopolysacharide (LPS) Challenge in Rats / Protocolo para realizar reto sistémico con lipopolisacárido (LPS) en ratas
Fonte: Odovtos (En línea);21(1):53-66, Jan.-Apr. 2019. tab, graf.
Idioma: en.
Resumo: Abstract 19. Lipopolysaccharide (LPS) is a component of the outer membrane of Gram-negative bacteria. In animals, intraperitoneal administration of LPS, stimulates innate immunity and the production of proinflammatory cytokines. LPS provides an inflammatory stimulus that activates the neuroimmune and neuroendocrine systems resulting in a set of responses termed sickness behavior. The purpose of this protocol is to describe step-by-step the preparation and procedure of application of intraperitoneal injection of LPS in rats, as a guide for those researchers that want to use this assay to mount an inflammatory response. LPS intraperitoneal challenge in rats has been widely used to evaluate antiinflammatory reagents and to address basic scientific questions.

Resumen 23. El lipopolisacárido (LPS) es un componente de la membrana externa de las bacterias Gram negativas. En animales, la administración intraperitoneal de LPS estimula la inmunidad innata y la producción de citoquinas proinflamatorias. El LPS proporciona un estímulo inflamatorio que activa el sistema neuroinmunológico y el sistema neuroendocrino, lo que da como resultado un conjunto de respuestas denominadas conductas de enfermedad. El propósito de este protocolo es describir paso a paso la preparación y el procedimiento de aplicación de la inyección intraperitoneal de LPS en ratas, como una guía para aquellos investigadores que desean utilizar este método para estimular una respuesta inflamatoria en el animal. La estimulación con LPS en ratas, aplicada intraperitonealmente, se ha utilizado ampliamente para evaluar reactivos antiinflamatorios y para abordar preguntas básicas de investigación científica.
Descritores: Lipopolissacarídeos/análise
Injeções Intraperitoneais/métodos
-Endotoxinas/análise
Bactérias Gram-Negativas
Limites: Animais
Ratos
Tipo de Publ: Protocolo de Ensaio Clínico
Responsável: CR1.1 - BINASSS - Biblioteca Nacional de Salud y Seguridad Social


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Id: biblio-990060
Autor: Merizalde, Gabriel; Lopera, Daniela; Villegas, Mauricio; Restrepo, Luz Marina.
Título: Leucocyte Platelet Rich Fibrin with Autologous Gingival Fibroblasts in the Treatment of Adjacent Recession Defects / Fibrina Rica en Plaquetas Leucocitarias con Fibroblastos Gingivales Autólogos en el Tratamiento de Defectos de Recesión Adyacentes
Fonte: Int. j. odontostomatol. (Print);13(1):23-30, mar. 2019. tab, graf.
Idioma: en.
Resumo: ABSTRACT: Periodontium can submit changes that lead to loss of integrity, such as periodontal disease, immune disorders or traumatic brushing. One of the most common consequences resulting from these events is the apical migration of gingival marginal tissue. Among biomaterials used for periodontal tissue regeneration, fibrin matrices have received significant attention to correct gingival recessions. Five oral mucosa biopsies were extracted, fibroblasts were in vitro cultured and frozen in liquid nitrogen. Three 10 mL glass sterile tubes were filled with patient blood and centrifuged immediately; clots were extracted and compressed to obtain L-PRF membranes. Autologous oral mucosa fibroblasts were added to the membranes and surgical procedures were performed in five patients. L-PRF fibrin network pore size was too small to allow human fibroblasts penetration but they were firmly attached to membrane surface. Gingival fibroblasts from fresh cell culture and recently thawed were used to attach on the L-PRF membranes. It was possible to establish a protocol for blood collection, centrifugation, fibrin clot compression, fibroblast adhesion to the membrane surface and patient application in a relatively short time (1 hour-1 hour and 30 minutes). Two patients expressed pain symptoms and the other ones presented light swelling without pain. In the first week, adjacent tissue showed few inflammation signs. Research efforts are being conducted to develop more conservative surgical techniques and new biomaterials that can promote cellular proliferation. Because of its properties, L-PRF membranes represent a tempting alternative. A combined technique to treat adjacent recession defects with L-PRF membranes and autologous oral mucosa fibroblasts in a coronal displaced flap did not show initial advantage compared with a gold standard surgery that includes an autologous soft tissue graft. Nevertheless, it could be an alternative for clinical application as a new functional cell biomaterial. More clinical evidence is needed.

RESUMEN: El periodonto puede presentar cambios que conducen a la pérdida de integridad, como la enfermedad periodontal, los trastornos inmunes o el cepillado traumático. Una de las consecuencias más comunes que resultan de estos eventos es la migración apical del tejido marginal gingival. Entre los biomateriales utilizados para la regeneración del tejido periodontal, las matrices de fibrina han recibido una atención significativa para corregir las recesiones gingivales. Se extrajeron cinco biopsias de mucosa oral, los fibroblastos se cultivaron in vitro y se congelaron en nitrógeno líquido. Tres tubos de vidrio estériles de 10 ml se llenaron con sangre del paciente y se centrifugaron inmediatamente. Los coágulos fueron extraídos y comprimidos para obtener membranas de L-PRF. Se agregaron fibroblastos autólogos de mucosa oral a las membranas y se realizaron procedimientos quirúrgicos en cinco pacientes. El tamaño de poro de la red de fibrina L-PRF era demasiado pequeño para permitir la penetración de los fibroblastos humanos, pero estaban firmemente unidos a la superficie de la membrana. Se usaron fibroblastos gingivales de cultivos de células frescas y recientemente descongelados para unirlos a las membranas L-PRF. Fue posible establecer un protocolo para la extracción de sangre, centrifugación, compresión de coágulos de fibrina, adhesión de fibroblastos a la superficie de la membrana y aplicación al paciente en un tiempo relativamente corto (1 hora, 1 hora y 30 minutos). Dos pacientes expresaron síntomas de dolor y los otros presentaron hinchazón leve sin dolor. En la primera semana, el tejido adyacente mostró pocos signos de inflamación. Se están realizando esfuerzos de investigación para desarrollar técnicas quirúrgicas más conservadoras y nuevos biomateriales que puedan promover la proliferación celular. Debido a sus propiedades, las membranas L-PRF representan una alternativa tentadora. Una técnica combinada para tratar los defectos de recesión adyacentes con membranas de L-PRF y fibroblastos de mucosa oral autóloga en un colgajo coronal desplazado no mostró una ventaja inicial en comparación con una cirugía estándar que incluye un injerto de tejido blando autólogo. Sin embargo, podría ser una alternativa para la aplicación clínica como un nuevo biomaterial de células funcionales. Se necesita más evidencia clínica.
Descritores: Estomatite
Lipopolissacarídeos/farmacologia
Citocinas/efeitos dos fármacos
Porphyromonas gingivalis/metabolismo
Fibroblastos/efeitos dos fármacos
Fibrina Rica em Plaquetas
Retração Gengival/cirurgia
-Cicatrização/fisiologia
Biópsia
Técnicas In Vitro
Periodonto
Microscopia Eletrônica
Comissão de Ética
Colômbia
Gengiva/citologia
Consentimento Livre e Esclarecido
Limites: Humanos
Masculino
Feminino
Adulto
Pessoa de Meia-Idade
Idoso
Responsável: CL1.1 - Biblioteca Central


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Id: biblio-1150993
Autor: Matos Uzcátegui, María Gabriela.
Título: Efecto de los antagonistas de los receptores AT1 de la angiotensina II sobre la enfermedad periodontal inducida por inyecciones de lipopolisacáridos en ratas / Effect of ang II AT1 receptor antagonists, on periodontal disease induced by injections of lipopolysaccharide in rats.
Fonte: Caracas; s.n; oct. 2012. ilus. (Ift4872012615853).
Idioma: es.
Tese: Apresentada a Universidad Central de Venezuela. Facultad de Farmacia para obtenção do grau de Doctor.
Símbolo: Ift4872012615853.
Resumo: La Enfermedad Periodontal (EP) es una condición inflamatoria progresiva que afecta los tejidos que soportan y rodean a los dientes. Las endotoxinas bacterianas como los lipopolisacáridos (LPS), inducen una cascada inflamatoria causando resorción ósea mediante la producción y modulación de la red de citoquinas del tejido periodontal, del sistema RANK-RANKL-OPG y de la producción de especies reactivas del oxígeno (ERO). Siendo la vía final la activación del factor de transcripción NFκB para el control de la infección. Se sabe que el Sistema Renina Angiotensina (SRA) esta involucrado en la inflamación. Estas acciones pro-inflamatorias de la Ang II son producidas por la activación de NFkB mediante los receptores AT1, y por la generación de ERO. Nuestro objetivo fue determinar si la inhibición del receptor AT1 con el uso del valsartán reduciría la respuesta inmunitaria innata inflamatoria en un modelo de EP inducida por las inyecciones de LPS en la encía de las ratas. Nuestros resultados demuestran que el Valsartán disminuyó la leucocitosis sistémica, la movilidad dentaria, atenuó la pérdida de peso corporal de las ratas, disminuyó la formación de enzimas antioxidantes y NOS, redujo la producción y liberación de citocinas pro inflamatorias y aumentó las citocinas antinflamatorias, disminuyó la activación de p-p38, p-NFkB y la expresión de los receptores TLR4. El Valsartán también revirtió los efectos del LPS sobre la resorción ósea ya que disminuyó el número de osteoclastos, la expresión de los receptores RANK/RANKL/OPG y la relación RANKL/OPG y aumentó los depósitos de calcio y colágeno. Los mecanismos por los cuales el Valsartán reduce los efectos inflamatorios producidos por el LPS no están claras, pero la interferencia del ensamblaje de la NAD(P)H oxidasa con apocinina y el Tempol, indica que el Valsartán puede interferir con los pasos para el reconocimiento de LPS y su asociación con TLR4. Concluyéndose que las ERO participan en la señalización intracelular de la ANG II, vía el AT1R. Este estudio ayuda a dilucidar el papel del SRA en procesos inflamatorios. Además contribuye con sus resultados a ofrecer una alternativa terapéutica en el tratamiento de la EP.
Descritores: Doenças Periodontais/induzido quimicamente
Lipopolissacarídeos/efeitos adversos
Bloqueadores do Receptor Tipo 1 de Angiotensina II/farmacologia
Valsartana/farmacologia
Gengiva/efeitos dos fármacos
-Doenças Periodontais/metabolismo
Doenças Periodontais/tratamento farmacológico
Periodontite/induzido quimicamente
Periodontite/tratamento farmacológico
Fatores de Tempo
Angiotensina II/efeitos adversos
Ratos Sprague-Dawley
Modelos Animais
Bloqueadores do Receptor Tipo 1 de Angiotensina II/uso terapêutico
Valsartana/uso terapêutico
Injeções
Limites: Animais
Masculino
Ratos
Responsável: VE497.1 - Biblioteca Dr. Oswaldo Enríquez Isava
VE497.1; D-CF, M37


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Id: biblio-1135491
Autor: Kuntjoro, Mefina; Prasetyo, Eric Priyo; Cahyani, Febriastuti; Kamadjaja, Michael Josef Kridanto; Hendrijantini, Nike; Laksono, Harry; Rahmania, Primanda Nur; Ariestania, Vivin; Nugraha, Alexander Patera; Ihsan, Igo Syaiful; Dinaryanti, Aristika; Rantam, Fedik Abdul.
Título: Lipopolysaccharides' Cytotoxicity on Human Umbilical Cord Mesenchymal Stem Cells
Fonte: Pesqui. bras. odontopediatria clín. integr;20:e0048, 2020. tab, graf.
Idioma: en.
Projeto: Lembaga Pengelola Dana Pendidikan Kementerian Keuangan Republik Indonesia; . Faculty of Dental Medicine Universitas Airlangga.
Resumo: Abstract Objective: To show the cytotoxicity of Porphyromonas gingivalis lipopolysaccharide (LPS) on human umbilical cord mesenchymal stem cells (HUCMSCs) to better understand the characteristics for its application in regenerative procedures under periodontopathogen LPS influence. Material and Methods: Ultrapure Porphyromonas gingivalis LPS was used in this study. This research used a frozen stock HUCMSCs, previously confirmed by flow cytometry. The biological characteristics, such as cell morphology, proliferation, and protein expression, were screened. To check the cytotoxicity, HUCMSCs were cultured and divided into two groups, the control group and LPS group with various concentrations from 25 to 0.39 µg/mL. MTT assay was done and the cells were observed and counted. The significance level was set at 5%. Results: The percentage of living HUCMSCs on LPS group were not significantly different among concentrations (p>0.05) from 25 to 0.39 µg/mL, even though there were slight mean decrease between groups, but they were not significant. The duration of 24 hours of exposure of LPS does not significantly lower HUCMSCs viability. Conclusion: LPS does not affect the viability of HUCMSCs. The lower the concentration of LPS, the higher the viability of HUCMSCs.
Descritores: Cordão Umbilical
Lipopolissacarídeos
Porphyromonas gingivalis
Citotoxicidade Imunológica/imunologia
Células-Tronco Mesenquimais
-Análise de Variância
Citometria de Fluxo
Indonésia/epidemiologia
Limites: Humanos
Tipo de Publ: Estudo Comparativo
Responsável: BR1264.1 - Biblioteca Setorial Prof Alberto M Campos


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Id: biblio-1133413
Autor: Siddiq, Muhammad; Wang, Fan; Xiao, Mi; Lin, Xiao Jie; Fatima, Nazira; Iqbal, Sara; Iqbal, Umar; Piao, Xian-Hua; Liu, Li.
Título: Inhibition of lipopolysaccharide-induced inflammation via the protective role of T regulatory cells in the fetal liver in a late-pregnancy preterm mouse model
Fonte: Clinics;75:e1665, 2020. tab, graf.
Idioma: en.
Resumo: OBJECTIVES: This study intended to explore the effect of T regulatory cells (Tregs) in the perinatal liver against LPS-induced inflammation in a preterm birth mouse model. Moreover, the role of adoptive Tregs on the inflammatory response induced by LPS was also studied. METHODS: Female BALB/C mice were injected intraperitoneally (IP) with LPS dissolved in normal saline solution at a dose of 50 µg/kg. Spleens from pregnant mice were used to obtain Tregs. The expression of Forkhead family transcription factor-3 (Foxp3), Interleukin-6 (IL-6), Toll-like receptor-4 (TLR-4), and Heme oxygenase-1 (HO-1) were assessed from fetal liver tissues by polymerase chain reaction and western blotting. RESULTS: LPS administered to mice induced an inflammatory response in the perinatal liver, and this inflammatory response was negatively regulated by Tregs in the experimental group. Maternal-fetal tolerance was maintained by Tregs. Transmission of Tregs was estimated in different experimental groups based on the mRNA expression of TLR-4, IL-6, HO-1, and Foxp3. CONCLUSIONS: After analysis of the experimental data, it was determined that Tregs exhibited regulatory potential against LPS-induced inflammatory response. Further, it was concluded that the transmission of Tregs improved the mother's immune tolerance against LPS-induced inflammation in the fetal liver.
Descritores: Lipopolissacarídeos/toxicidade
Nascimento Prematuro
-Linfócitos T Reguladores
Fatores de Transcrição Forkhead
Inflamação/induzido quimicamente
Fígado
Camundongos Endogâmicos BALB C
Limites: Animais
Feminino
Gravidez
Camundongos
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


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Id: biblio-950765
Autor: Park, Hee-Sook; Choi, Hye-Young; Kim, Gun-Hee.
Título: Preventive effect of Ligularia fischeri on inhibition of nitric oxide in lipopolysaccharide-stimulated RAW 264. 7 macrophages depending on cooking method
Fonte: Biol. Res;47:1-6, 2014. graf, tab.
Idioma: en.
Projeto: Globalization of Korean Foods R& D program; . Ministry of Agriculture, Food, and Rural Affairs, Republic of Korea; . National Research Foundation of Korea; . Ministry of Education. Basic Science Research Program.
Resumo: BACKGROUND: Ligularia fischeri (common name Gomchwi) is known for its pharmaceutical properties and used in the treatment of jaundice, scarlet-fever, rheumatoidal arthritis, and hepatic diseases; however, little is known about its anti-inflammatory effect. In this study the influence of blanching and pan-frying on the anti-inflammatory activity of Ligularia fischeri (LF) was evaluated. RESULTS: Fresh LF and cooked LF showed no significant effect on the viability of macrophages after 24 h incubation. Fresh LF was found to be the most potent inhibitor of nitric oxide (NO) production at 100 µg/ml, while pan-fried LF showed little inhibitory effect on lipoloysaccharide (LPS) stimulated murine machrophage RAW264.7 cells. In contrast with its effect on NO production, pan-fried LF showed significant attenuation of the expression of inducible nitiric oxide synthase (iNOS) compared with fresh LF. In the cooking method of LF, PGE2 production was not affected in the LPS-induced RAW 264.7 cells. In LPS-induced RAW 264.7 cells, pretreatment by fresh and cooked LF increased COX2 mRNA expression. The 3-O-caffeoylquinic acid content of blanching and pan-frying LF increased by 4.92 and 9.7 fold with blanching and pan-frying respectively in comparison with uncooked LF. CONCLUSIONS: Regardless of the cooking method, Ligularia fischeri exhibited potent inhibition of NO production through expression of iNOS in LPS-induced RAW264.7 cells.
Descritores: Culinária/métodos
Asteraceae/química
Preparações de Plantas/farmacologia
Óxido Nítrico Sintase Tipo II/metabolismo
Macrófagos/efeitos dos fármacos
Óxido Nítrico/biossíntese
-Ácido Quínico/análise
Ácido Quínico/análogos & derivados
Ácido Quínico/classificação
RNA Mensageiro/efeitos dos fármacos
RNA Mensageiro/metabolismo
Dinoprostona/análise
Dinoprostona/biossíntese
Sobrevivência Celular
Lipopolissacarídeos
Cromatografia Líquida de Alta Pressão
Asteraceae/classificação
Ciclo-Oxigenase 2/análise
Ciclo-Oxigenase 2/metabolismo
Células RAW 264.7
Temperatura Alta
Macrófagos/fisiologia
Anti-Inflamatórios/farmacologia
Limites: Animais
Camundongos
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: CL1.1 - Biblioteca Central


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Id: biblio-950776
Autor: Thao, Nguyen Phuong; Luyen, Bui Thi Thuy; Koo, Jung Eun; Kim, Sohyun; Koh, Young Sang; Cuong, Nguyen Xuan; Nam, Nguyen Hoai; Van Kiem, Phan; Kim, Young Ho; Van Minh, Chau.
Título: Anti-inflammatory components of the Vietnamese starfish Protoreaster nodosus
Fonte: Biol. Res;48:1-9, 2015. ilus, graf, tab.
Idioma: en.
Projeto: Vietnam Academy of Science and Technology; . National Research Foundation of Korea; . Ministry of Education, Science, and Technology.
Resumo: BACKGROUND: In the present study, we examined the inhibitory effects of a methanolic extract, dichloromethane fraction, water layer, and polyhydroxylated sterols (1-4) isolated from the Vietnamese starfish Protoreaster nodosus on pro-inflammatory cytokine (IL-12 p40, IL-6, and TNF-α) production in LPS-stimulated bone marrow-derived dendritic cells (BMDCs) using enzyme-linked immunosorbent assays (ELISA). RESULTS: The methanolic extract and dichloromethane fraction exerted potent inhibitory effects on the production of all three pro-inflammatory cytokines, with IC50 values ranging from 0.60 ± 0.01 to 26.19 ± 0.64 µg/mL. Four highly pure steroid derivatives (1-4) were isolated from the dichloromethane fraction and water layer of P. nodosus. Potent inhibitory activities were also observed for (25S)5α-cholestane-3ß,4ß,6α,7α,8ß,15α,16ß,26-octol (3) on the production of IL-12 p40 and IL-6 (IC50s = 3.11 ± 0.08 and 1.35 ± 0.03 µM), and for (25S) 5α-cholestane-3ß,6α,8ß,15α,16ß,26-hexol (1) and (25S)5α-cholestane-3ß,6α,7α,8ß,15α,16ß,26-heptol (2) on the production of IL-12 p40 (IC50s = 0.01 ± 0.00 and and 1.02 ± 0.01 µM). Moreover, nodososide (4) exhibited moderate inhibitory effects on IL-12 p40 and IL-6 production. CONCLUSION: This is the first report of the anti-inflammatory activity from the starfish P. nodosus. The main finding of this study is the identification oxygenated steroid derivatives from P. nodosus with potent anti-inflammatory activities that may be developed as therapeutic agents for inflammatory diseases.
Descritores: Estrelas-do-Mar/química
Células Dendríticas/efeitos dos fármacos
Interleucina-6/farmacologia
Fator de Necrose Tumoral alfa/farmacologia
Subunidade p40 da Interleucina-12/farmacologia
Anti-Inflamatórios/análise
-Esteroides/administração & dosagem
Vietnã
Ensaio de Imunoadsorção Enzimática
Sobrevivência Celular/efeitos dos fármacos
Lipopolissacarídeos
Interleucina-6/análise
Fator de Necrose Tumoral alfa/análise
Concentração Inibidora 50
Subunidade p40 da Interleucina-12/análise
Cultura Primária de Células
Camundongos Endogâmicos C57BL
Limites: Animais
Camundongos
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: CL1.1 - Biblioteca Central


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Id: biblio-1132197
Autor: Ávila, Caroline Nickel; Trindade, Fernanda Moura Ribeiro; Penteado, Júlia Oliveira; Janke, Fernanda; Schneider, Janaína Pereira; Uecker, Júlia Neitzel; Rincón, Joao Alveiro Alvarado; Barros, Carlos Castilho de; Andreazza, Robson; Pieniz, Simone.
Título: Anti-inflammatory Effect of a Goji Berry Extract (Lycium barbarum) in Rats Subjected to Inflammation by Lipopolysaccharides (LPS)
Fonte: Braz. arch. biol. technol;63:e20180612, 2020. tab, graf.
Idioma: en.
Projeto: Coordenação de Aperfeiçoamento de Pessoal de Nível Superior.
Resumo: Abstract The present study aimed to evaluate the anti-inflammatory potential of a Lycium barbarum (L. barbarum) fruit extract in Wistar rats submitted to a palatable diet presenting systemic inflammation induced by lipopolysaccharides (LPS). Forty-two Wistar female rats (Rattus Novergicus) were used with 60 days old. The animals were feed for 60 days and divided in six groups (n=7): standard diet+water; standard diet+L. barbarum; palatable diet+water; palatable diet+L. barbarum; standard diet+water+LPS; standard diet+L. barbarum+LPS. A significant difference was shown between the analyzed groups concerning C-reactive protein, with the standard diet+water+LPS group presenting the highest inflammatory response in comparison to the other groups. Decreased inflammatory response was observed in the group administered a palatable diet along with the fruit extract when compared to the group that received only a palatable diet. Significant decrease in glutamic-oxaloacetic transaminase activity was observed in the standard diet+L. barbarum+LPS group compared to the standard diet+water group, as well as in the palatable diet+L. barbarum group compared to the palatable diet+water group. A significant increase in creatinine in the standard diet+water+LPS group was observed in according to the L. barbarum administration groups. The gene expression of the inflammatory markers genes in the liver showed a significant increase in TNF-α and IL-6 genes in the group treated with standard diet+L. barbarum+LPS when compared to the standard diet+LPS group. Thus, the administered L. barbarum extract displays the potential to reduce inflammatory responses induced by LPS and a palatable diet.
Descritores: Lycium
Inflamação/tratamento farmacológico
Anti-Inflamatórios/farmacologia
-Extratos Vegetais
Lipopolissacarídeos/efeitos adversos
Ratos Wistar
Alanina Transaminase
Modelos Animais de Doenças
Inflamação/microbiologia
Limites: Animais
Feminino
Ratos
Responsável: BR1.1 - BIREME


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Id: biblio-950821
Autor: Dzoyem, Jean P; Tsamo, Armelle T; Melong, Raduis; Mkounga, Pierre; Nkengfack, Augustin E; McGaw, Lyndy J; Eloff, Jacobus N.
Título: Cytotoxicity, nitric oxide and acetylcholinesterase inhibitory activity of three limonoids isolated from Trichilia welwitschii (Meliaceae)
Fonte: Biol. Res;48:1-6, 2015. ilus, graf, tab.
Idioma: en.
Resumo: BACKGROUND: Limonoids are highly oxygenated compounds with a prototypical structure. Their occurrence in the plant kingdom is mainly confined to plant families of Meliaceae and Rutaceae. Owing to their wide range of pharmacological and therapeutic properties, this study was aimed at investigating the potential nitric oxide (NO) and acetylcholinesterase (AChE) inhibitory activity and the cytotoxicity of three limonoids: trichilia lactone D5 (1), rohituka 3 (2) and dregeanin DM4 (3), isolated from Trichilia welwitschii C.DC. RESULTS: Results indicated that the three limonoids had low cytotoxicity towards Vero cells with LC50 values ranging from 89.17 to 75.82 µg/mL. Compounds (2) and (3) had lower cytotoxicity compared to puromycin and doxorubicin used as reference cytotoxic compounds. Compound (1) (LC50 of 23.55 µg/mL) had good antiproliferative activity against RAW 264.7 cancer cells. At the lowest concentration tested (0.5 µg/mL), compound (2) and (3) released the lowest amount of nitric oxide (2.97 and 2.93 µM, respectively). The three limonoids had anti-AChE activity with IC50 values ranged of 19.13 µg/mL for (1), 34.15 µg/mL for (2) and 45.66 µg/mL for (3), compared to galantamine (IC50 of 8.22 µg/mL) used as positive control. CONCLUSION: The limonoid compounds studied in this work inhibited nitric oxide production in LPS-stimulated macrophages and had anti-AChE activity. Trichilia lactone D5 had potential antiproliferative activity against RAW 264.7 cancer cells. The limonoids had low cytotoxicity towards Vero cells lines. This study provided further examples of the importance of limonoids compounds as potential AChE inhibitors and anti-inflammatory agents targeting the inhibition of NO production.
Descritores: Inibidores da Colinesterase/farmacologia
Meliaceae/química
Limoninas/farmacologia
Óxido Nítrico/antagonistas & inibidores
-Células Vero
Chlorocebus aethiops
Lipopolissacarídeos
Concentração Inibidora 50
Limoninas/isolamento & purificação
Limoninas/análise
Proliferação de Células/efeitos dos fármacos
Células RAW 264.7
Lactonas/análise
Lactonas/farmacologia
Dose Letal Mediana
Macrófagos/efeitos dos fármacos
Anti-Inflamatórios/farmacologia
Óxido Nítrico/análise
Limites: Animais
Camundongos
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: CL1.1 - Biblioteca Central


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Id: biblio-950833
Autor: Shu, Jun; He, Xiaojuan; Zhang, Lan; Li, Hong; Wang, Ping; Huang, Xiaojie.
Título: Human amnion mesenchymal cells inhibit lipopolysaccharide-induced TNF-α and IL-1ß production in THP-1 cells
Fonte: Biol. Res;48:1-8, 2015. ilus, graf.
Idioma: en.
Projeto: Grants from China-Japan Friendship Hospital.
Resumo: BACKGROUND: Human amnion mesenchymal cells (hAMCs), isolated from the amniotic membrane of human placenta, are a unique population of mesenchymal stem cells. Recent studies demonstrated that hAMCs could inhibit the activities and functions of several immune cells. However, their effect on inflammatory macrophages is largely unknown. This study investigated the effect of hAMCs on expression of inflammatory cytokines and mitogen-activated protein kinases (MAPKs)/NF-kB pathway in human THP-1 macrophages induced by lipopolysaccharide (LPS). RESULTS: The levels of TNF-α and IL-1ß secreted by LPS- stimulated THP-1 cells were increased significantly compared with those in the control group. After co-culture with different numbers of hAMCs, the levels of TNF-α and IL-1ß in LPS-stimulated THP-1 cells were significantly reduced compared with the LPS group. The mRNA expression of TNF-α and IL-1ß were also markedly inhibited. Moreover, treating LPS-stimulated THP-1 cells with hAMCs supernatants could also suppress TNF-α and IL-1ß production in THP-1 cells. Important signaling pathways involved in the production of TNF-α and IL-1ß were affected by hAMCs co-culture: hAMCs remarkably suppressed NF-kB activation and down-regulated the phosphorylation of ERK and JNK in LPS- stimulated THP-1 cells. CONCLUSIONS: Human amnion mesenchymal cells inhibited the production of TNF-α and IL-1ß secreted by LPS-stimulated THP-1 cells, partly through the suppression of NF-kB activation and ERK and JNK phosphorylation.
Descritores: Lipopolissacarídeos/farmacologia
Fator de Necrose Tumoral alfa/biossíntese
Interleucina-1beta/biossíntese
Células-Tronco Mesenquimais/fisiologia
Âmnio/citologia
Macrófagos/metabolismo
-Fator de Necrose Tumoral alfa/efeitos dos fármacos
Sistema de Sinalização das MAP Quinases/efeitos dos fármacos
Interleucina-1beta/efeitos dos fármacos
Limites: Humanos
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: CL1.1 - Biblioteca Central



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