Base de dados : LILACS
Pesquisa : D09.400.500 [Categoria DeCS]
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Id: lil-785018
Autor: Guo, Yingfei; Ma, Lingyun; Zhang, Fei; Sun, Rongju; Li, Tanshi.
Título: Neutrophil elastase ameliorates matrix metalloproteinase-9 to promote lipopolysaccharide-induced acute lung injury in mice 1
Fonte: Acta cir. bras;31(6):382-388graf.
Idioma: en.
Projeto: National Natural Science project of China.
Resumo: ABSTRACT PURPOSE: To investigate the regulatory roles of neutrophil elastase (NE) and matrix metalloproteinase-9 (MMP-9) in lipopolysaccharide (LPS)-induced acute lung injury (ALI) in mice. METHODS: To construct LPS-induced ALI mouse models, wild-type C57BL/6 mice were administered 5.0 mg/kg of LPS through endotracheal, and/or 1.0 mg/kg of ONO-5046, and/or 20.0 mg/kg of chemically modified tetracycline-3 (CMT-3) by gavage. The levels of MMP-9, tissue inhibitor of metalloprotease-1, interleukin (IL)-6 were detected by real time RT-PCR at 6 h, 24 h and 48 h, and tumor necrosis factor (TNF), lung wet-dry weight ratio, white blood cell (WBC) count and polymorphonuclear (PMN) count in bronchoalveolar lavage fluid (BALF) were tested at 48 h after administration. The 5-day survival analysis of the ALI mice was also performed. RESULTS: Both ONO-5046 and CMT-3, regardless of being used individually or combined, significantly reduced the levels of MMP-9, IL-6, and TNF in lung tissue as well as in BALF, and the WBC and PMN count in BALF. Combined treatment with ONO-5046 and CMT-3 remarkably improved the survival rate of ALI mice. CONCLUSION: Neutrophil elastase synergizes with matrix metalloproteinase-9 to promote and regulate the release of inflammatory mediators and the infiltration of inflammatory cells, consequently affecting the survival of lipopolysaccharide-induced acute lung injury mice.
Descritores: Sulfonamidas/administração & dosagem
Tetraciclinas/administração & dosagem
Elastase de Leucócito/metabolismo
Metaloproteinase 9 da Matriz/metabolismo
Lesão Pulmonar Aguda/enzimologia
Glicina/análogos & derivados
-Fatores de Tempo
Líquido da Lavagem Broncoalveolar/citologia
Análise de Sobrevida
Lipopolissacarídeos
Interleucina-6/metabolismo
Mediadores da Inflamação/metabolismo
Elastase de Leucócito/efeitos dos fármacos
Inibidor Tecidual de Metaloproteinase-1/metabolismo
Metaloproteinase 9 da Matriz/análise
Metaloproteinase 9 da Matriz/efeitos dos fármacos
Fatores de Necrose Tumoral/metabolismo
Modelos Animais de Doenças
Lesão Pulmonar Aguda/induzido quimicamente
Lesão Pulmonar Aguda/sangue
Glicina/administração & dosagem
Contagem de Leucócitos
Camundongos Endogâmicos C57BL
Neutrófilos
Limites: Animais
Responsável: BR1.1 - BIREME


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Id: lil-743785
Autor: Yang, Zhi; Xiao, Ke; Wang, Wei; Tang, Juan; Sun, Peng-Peng; Peng, Ke-Mei; Song, Hui.
Título: The effect of visfatin on inflammatory reaction in uterus of LPS-induced rats / Efecto de visfatina en la reacción inflamatoria en el útero de ratas inducidas por LPS
Fonte: Int. j. morphol;33(1):194-203, Mar. 2015. ilus.
Idioma: en.
Projeto: National Natural Science FundProject of China.
Resumo: The present study was to investigate the effects of visfatin on the morphological structure and function of the rat uterus during inflammation. The expression and distribution of visfatin, morphological structure, eosinophils (EOS), myeloperoxidase (MPO) and cytokines in the uterus of the LPS-induced rat were studied using hematoxylin-eosin staining (HE), immunohistochemical methods, western blots and enzyme-linked immunosorbent assay (ELISA). The present study showed that visfatin positive cells dispersed widely in the uterus, and strong positive staining was observed mainly in the cell cytoplasm. Compared with saline group, in visfatin group, more uterine glands were found, EOS increased, and the difference was significant (P<0.05), MPO reduced, and the difference was significant (P<0.01). In addition, visfatin was able to increase the secretion of IL-1b, IL-6, and TNF-a (P<0.01). Compared with LPS group, in vifatin+LPS group, the uterine glands of the lamina propria increased, the myometrium became thinner, the number of EOS and MPO reduced obviously, but the difference was not significant (P>0.05), and after LPS stimulated body, visfatin decrease the level of IL-1b, IL-6, TNF-a (P<0.01). The above results suggest that visfatin could affect the morphological structure of rat uterus; Visfatin could modulate the inflammatory response in rats' uterus by regulating the quantity of inflammatory cells, such as EOS and MPO, and the level of inflammatory cytokines, such as IL-1b, IL-6, TNF-a.

El objetivo del presente estudio fue investigar los efectos de la visfatina sobre la estructura morfológica y la función del útero de la rata durante la inflamación. Se estudiaron la expresión y distribución de la visfatina, la estructura morfológica, eosinófilos, mieloperoxidasa y citoquinas en el útero de rata mediante la tinción de H&E, métodos inmunohistoquímicos, Western blots y ELISA. El estudio mostró que las células visfatina positivas se dispersan ampliamente en el útero, junto a una fuerte tinción positiva, principalmente en el citoplasma de la célula. En comparación con el grupo control, en el grupo visfatina, se encontraron más glándulas uterinas, se observó un aumento de EOS y la diferencia fue significativa (p<0,05), MPO reducida siendo esta diferencia también significativa (p<0,01). Además, la visfatina fue capaz de aumentar la secreción de IL-1b, IL-6 y TNF-a (P<0,01). En comparación con el grupo LPS, visfatina+grupo LPS, las glándulas uterinas de la lámina propia aumentaron, se observó un miometrio más delgado, y número reducido de EOS y MPO, sin embargo, la diferencia no fue significativa (P>0,05). Después de estímulo LPS en el cuerpo, se registró un nivel menor de visfatina en IL-1b, IL-6, TNF-a (P<0,01). Los resultados anteriores sugieren que visfatina podría afectar a la estructura morfológica del útero de rata. Además, podría modular la respuesta inflamatoria en el útero mediante la regulación de la cantidad de células inflamatorias, tales como EOS y MPO.
Descritores: Útero/efeitos dos fármacos
Lipopolissacarídeos/toxicidade
Nicotinamida Fosforribosiltransferase/farmacologia
-Ensaio de Imunoadsorção Enzimática
Imuno-Histoquímica
Western Blotting
Ratos Wistar
Peroxidase/efeitos dos fármacos
Inflamação
Neutrófilos/efeitos dos fármacos
Limites: Animais
Feminino
Ratos
Responsável: CL1.1 - Biblioteca Central


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Silva, Célio Lopes
Silva, Léa Assed Bezerra da
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Id: biblio-893638
Autor: ROMUALDO, Priscilla Coutinho; GUERRA, Thaís Rodrigues; ROMANO, Fábio Lourenço; SILVA, Raquel Assed Bezerra da; BRANDÃO, Izaíra Tincani; SILVA, Célio Lopes; SILVA, Lea Assed Bezerra da; NELSON-FILHO, Paulo.
Título: Bacterial endotoxin adhesion to different types of orthodontic adhesives
Fonte: J. appl. oral sci;25(4):436-441, July-Aug. 2017. tab, graf.
Idioma: en.
Projeto: Fundação de Amparo à Pesquisa do Estado de São Paulo.
Resumo: Abstract Bacterial endotoxin (LPS) adhesion to orthodontic brackets is a known contributing factor to inflammation of the adjacent gingival tissues. Objective The aim of this study was to assess whether LPS adheres to orthodontic adhesive systems, comparing two commercial brands. Material and Methods Forty specimens were fabricated from Transbond XT and Light Bond composite and bonding agent components (n=10/component), then contaminated by immersion in a bacterial endotoxin solution. Contaminated and non-contaminated acrylic resin samples were used as positive and negative control groups, respectively. LPS quantification was performed by the Limulus Amebocyte Lysate QCL-1000™ test. Data obtained were scored and subjected to the Chi-square test using a significance level of 5%. Results There was endotoxin adhesion to all materials (p<0.05). No statistically significant difference was found between composites/bonding agents and acrylic resin (p>0.05). There was no significant difference (p>0.05) among commercial brands. Affinity of endotoxin was significantly greater for the bonding agents (p=0.0025). Conclusions LPS adhered to both orthodontic adhesive systems. Regardless of the brand, the endotoxin had higher affinity for the bonding agents than for the composites. There is no previous study assessing the affinity of LPS for orthodontic adhesive systems. This study revealed that LPS adheres to orthodontic adhesive systems. Therefore, additional care is recommended to orthodontic applications of these materials.
Descritores: Aderência Bacteriana/fisiologia
Lipopolissacarídeos/fisiologia
Resinas Compostas/química
Cimentos de Resina/química
Escherichia coli
-Valores de Referência
Teste de Materiais
Ensaio de Imunoadsorção Enzimática
Lipopolissacarídeos/isolamento & purificação
Tipo de Publ: Estudo Comparativo
Responsável: BR1.1 - BIREME


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Id: biblio-842788
Autor: Miyamoto, Maristela; Gouvêa, Aída FTB; Ono, Erika; Succi, Regina Célia M; Pahwa, Savita; Moraes-Pinto, Maria Isabel de.
Título: Immune development in HIV-exposed uninfected children born to HIV-infected women
Fonte: Rev. Inst. Med. Trop. Säo Paulo;59:e30, 2017. tab, graf.
Idioma: en.
Projeto: Fundação de Amparo à Pesquisa do Estado de São Paulo; . Fundação de Amparo à Pesquisa do Estado de São Paulo.
Resumo: ABSTRACT Immunological and clinical findings suggestive of some immune dysfunction have been reported among HIV-exposed uninfected (HEU) children and adolescents. Whether these defects are persistent or transitory is still unknown. HEU pediatric population at birth, 12 months, 6-12 years were evaluated in comparison to healthy age-matched HIV-unexposed controls. Plasma levels of LPS, sCD14, cytokines, lymphocyte immunophenotyping and T-cell receptor excision circles (TREC) were assessed. HEU and controls had similar LPS levels, which remained low from birth to 6-12 years; for plasma sCD14, IL-2, IL-6, IL-7, IL-10, IL-12p70, IL-13, IL-17, IFN-γ, TNF-α, G-CSF, GM-CSF and MCP-1, which increased from birth to 12 months and then decreased at 6-12 years; and for TREC/106 PBMC at birth in HEU and controls. By contrast, plasma MIP-1β levels were lower in HEU than in controls (p=0.009) at 12 months, and IL-4 levels were higher in HEU than controls (p=0.04) at 6-12 years. Immune activation was higher in HEU at 12 months and at 6-12 years than controls based on frequencies of CD38+HLA-DR+CD8+T cells (p=0.05) and of CD38+HLA-DR+CD4+T cells (p=0.006). Resting memory and activated mature B cells increased from birth to 6-12 years in both groups. The development of the immune system in vertically HEU individuals is comparable to the general population in most parameters, but subtle or transient differences exist. Their role in influencing clinical incidences in HEU is unknown.
Descritores: Complicações Infecciosas na Gravidez/imunologia
Infecções por HIV/imunologia
Lipopolissacarídeos/sangue
Citocinas/sangue
Contagem de Linfócito CD4
Receptores de Lipopolissacarídeos/sangue
-Valores de Referência
Fatores de Tempo
Biomarcadores/sangue
Estudos de Casos e Controles
Lipopolissacarídeos/imunologia
Citocinas/imunologia
Exposição Materna
Receptores de Lipopolissacarídeos/imunologia
Citometria de Fluxo
Memória Imunológica
Limites: Seres Humanos
Masculino
Feminino
Gravidez
Recém-Nascido
Lactente
Criança
Responsável: BR1.1 - BIREME


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Id: biblio-841165
Autor: LIU, Zhiqiang; HU, Yang; YU, Pei; LIN, Mei; HUANG, Grace; KAWAI, Toshihisa; TAUBMAN, Martin; WANG, Zuomin; Xiaozhe, HAN.
Título: Toll-like receptor agonists Porphyromonas gingivalis LPS and CpG differentially regulate IL-10 competency and frequencies of mouse B10 cells
Fonte: J. appl. oral sci;25(1):90-100, Jan.-Feb. 2017. tab, graf.
Idioma: en.
Projeto: NIH NIDCR; . NIH NIDCR.
Resumo: Abstract IL-10 expressing regulatory B cells (B10) play a key role in immune system balance by limiting excessive inflammatory responses. Effects of toll-like receptor signaling and co-stimulatory molecules on B10 activity during innate and adaptive immune responses are not fully understood. Objective This study is to determine the effects of P. gingivalis LPS and CpG on B10 cell expansion and IL-10 competency in vitro. Material and Methods Spleen B cells were isolated from C57BL/6J mice with or without formalin-fixed P. gingivalis immunization. B cells were cultured for 48 hours under the following conditions: CD40L, CD40L+LPS, CD40L+CpG, and CD40L+LPS+CpG in the presence or absence of fixed P. gingivalis. Percentages of CD1dhiCD5+ B cells were measured by flow cytometry. IL-10 mRNA expression and secreted IL-10 were measured by real-time quantitative PCR and by ELISA respectively. Results P. gingivalis LPS plus CD40L significantly increased CD1dhiCD5+ B cell percentages and secreted IL-10 levels in both immunized and non-immunized mice B cells in the presence or absence of P. gingivalis, compared with control group. Secreted IL-10 levels were significantly increased in CD40L+LPS treated group compared with CD40L treatment group in the absence of P. gingivalis. CpG plus CD40L significantly decreased CD1dhiCD5+ B cell percentages, but greatly elevated secreted IL-10 levels in immunized and non-immunized mice B cells in the absence of P. gingivalis, compared with CD40L treatment group. Conclusions P. gingivalis LPS and CpG differentially enhance IL-10 secretion and expansion of mouse B10 cells during innate and adaptive immune responses.
Descritores: Lipopolissacarídeos/fisiologia
Interleucina-10/imunologia
Porphyromonas gingivalis/fisiologia
Ligante de CD40/fisiologia
Receptor Toll-Like 9/agonistas
Receptor 4 Toll-Like/agonistas
Linfócitos B Reguladores/imunologia
-Baço/citologia
Fatores de Tempo
RNA Mensageiro/análise
Ensaio de Imunoadsorção Enzimática
Distribuição Aleatória
Células Cultivadas
Interleucina-10/análise
Interleucina-10/metabolismo
Receptor Toll-Like 9/fisiologia
Receptor 4 Toll-Like/fisiologia
Reação em Cadeia da Polimerase em Tempo Real
Imunidade Inata
Camundongos Endogâmicos C57BL
Limites: Animais
Responsável: BR1.1 - BIREME


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Id: biblio-839523
Autor: ALBIERO, Mayra Laino; AMORIM, Bruna Rabelo; CASATI, Márcio Zaffalon; SALLUM, Enilson Antonio; NOCITI JUNIOR, Francisco Humberto; SILVÉRIO, Karina Gonzales.
Título: Osteogenic potential of periodontal ligament stem cells are unaffected after exposure to lipopolysaccharides
Fonte: Braz. oral res. (Online);31:e17, 2017. tab, graf.
Idioma: en.
Projeto: São Paulo State Research Foundation.
Resumo: Abstract Periodontitis develops as a result of a continuous interaction between host cells and subgingival pathogenic bacteria. The periodontium has a limited capacity for regeneration, probably due to changes in periodontal ligament stem cells (PDLSCs) phenotype. The aim of this study was to evaluate the effects of lipopolysaccharides from Porphyromonas gingivalis (PgLPS) on mesenchymal phenotype and osteoblast/cementoblast (O/C) potential of PDLSCs. PDLSCs were assessed for Toll-like receptor 2 (TLR2) expression by immunostaining technique. After, cells were exposed to PgLPS, and the following assays were carried out: (i) cell metabolic activity using MTS; (ii) gene expression for IL-1β, TNF-α and OCT-4 by real-time polymerase chain reaction (RT-qPCR); (iii) flow cytometry for STRO-1 and CD105, and (iv) osteogenic differentiation. PDLSCs were positive for TLR2. PgLPS promoted cell proliferation, produced IL-1β and TNF-α, and did not affect the expression of stem cell markers, STRO-1, CD105 and OCT-4. Under osteogenic condition, PDLSCs exposed to PgLPS showed a similar potential to differentiate toward osteoblast/cementoblast phenotype compared to control group as revealed by mineralized matrix deposition and levels of transcripts for RUNX2, ALP and OCN. These results provide evidence that PgLPS induces pro-inflammatory cytokines, but does not change the mesenchymal phenotype and osteoblast/cementoblast differentiation potential of PDLSCs.
Descritores: Osteogênese/efeitos dos fármacos
Ligamento Periodontal/citologia
Lipopolissacarídeos/toxicidade
Porphyromonas gingivalis
Células Mesenquimais Estromais/efeitos dos fármacos
-Fatores de Tempo
Expressão Gênica
Osteocalcina/análise
Diferenciação Celular/efeitos dos fármacos
Sobrevivência Celular/efeitos dos fármacos
Células Cultivadas
Fator de Necrose Tumoral alfa/análise
Estatísticas não Paramétricas
Proliferação Celular/efeitos dos fármacos
Fosfatase Alcalina/análise
Fator 3 de Transcrição de Octâmero/análise
Receptores Toll-Like/análise
Subunidade alfa 1 de Fator de Ligação ao Core/análise
Interleucina-1beta/análise
Células Mesenquimais Estromais/metabolismo
Reação em Cadeia da Polimerase em Tempo Real
Citometria de Fluxo
Limites: Seres Humanos
Responsável: BR1.1 - BIREME


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Id: biblio-1015337
Autor: Bonvini, Andrea.
Título: Efeitos dos aminoácidos de cadeia ramificada na resposta inflamatória induzida por lipopolissacarídeo em macrófagos de linhagem celular RAW 264. 7 / Effects of branched-chain amino acids in the inflammatory response induced by lipopolysaccharide in a macrophage cell line RAW 264. 7.
Fonte: São Paulo; s.n; 2019. 87 p. graf, tab.
Idioma: pt.
Tese: Apresentada a Universidade de São Paulo. Faculdade de Ciências Farmacêuticas para obtenção do grau de Doutor.
Resumo: Os aminoácidos de cadeia ramificada (ACR) são considerados indispensáveis, pois não podem ser sintetizados endogenamente, sendo facilmente obtidos pela dieta. Entretanto, em determinadas condições clínicas, tanto a ingestão quando a absorção desses aminoácidos pode estar comprometida, levando ao estado hipercatabólico e prejudicando a função imune. O papel imunomodulador dos ACR tem sido relacionado com a melhora no balanço nitrogenado e o aumento da síntese e proliferação de células imunes, bem como, da síntese de mediadores inflamatórios. Entretanto, o mecanismo pelo qual os ACR exercem essas funções supracitadas ainda não é claro na literatura científica. Desta forma, esse trabalho teve como objetivo avaliar os efeitos da suplementação com ACR sobre os parâmetros inflamatórios e moleculares em macrófagos RAW 264.7 estimulados com lipopolissacarídeo (LPS). As culturas celulares foram distribuídas em cinco grupos: CTL - sem suplementação com ACR; LEU - suplementado com leucina (2 mmol/L); ISO - suplementado com isoleucina (2mmol/L); VAL - suplementado com valina (2 mmol/L) e LIV - suplementado com leucina (2 mmol/L), isoleucina (2 mmol/L) e valina (2 mmol/L). O estado inflamatório foi induzido pela adição de LPS (1 µg/mL) ao meio de cultura, seguindo quatro protocolos de tratamento: PT - pré-tratamento; TA - tratamento agudo; TC - tratamento crônico e TT - tratamento tardio. O ensaio de viabilidade celular foi realizado pelo teste MTT e a dosagem de óxido nítrico (NO) pela reação de Griess. As citocinas pró e anti-inflamatórias, e a prostaglandina E2 (PGE2) foram analisadas pelo método de ELISA. Para a avaliação dos parâmetros moleculares foi utilizado o método de western blotting. Houve aumento da viabilidade celular em todos os grupos suplementados em relação ao grupo controle no TA, no TC e no TT. Acerca da síntese de NO, a suplementação com ACR foi capaz de aumentar esse parâmetro em três dos quatro tratamentos propostos (PT, TA e TC). Em relação à síntese de citocinas pró e anti-inflamatórias, o PT e o TC foram mais eficazes em aumentar esse parâmetro em comparação aos outros tratamentos. Não houve diferença entre os grupos em relação à capacidade de síntese de PGE2 e à fosforilação de proteínas intracelulares. A partir dos resultados obtidos é possível concluir que os ACR contribuem significativamente para a viabilidade celular, bem como para a síntese de mediadores pró e anti-inflamatórios, sendo que o protocolo de suplementação se apresenta como fator determinante para obtenção desses resultados. Apesar da literatura científica atribuir grande parte dos efeitos imunomodulatórios à leucina, os resultados obtidos nesse estudo atribuem relevante potencial imunomodulador à isoleucina, abrindo espaço para um importante tema de estudo

Branched chain amino acids (BCAA) are considered indispensable, since they cannot be endogenously synthesized, being easily obtained by diet. However, in certain clinical conditions, both the intake and absorption of these amino acids may be compromised, leading to the hypercatabolic state and impairing the immune function. The immunomodulatory role of BCAA has been associated with the nitrogen balance improvement and the increase of production and proliferation of immune cells, as well as the synthesis of inflammatory mediators. However, the mechanisms by which BCAA modulate the immune system have not yet been completely elucidated. In this sense, this study aimed to evaluate the effects of BCAA supplementation on intracellular mechanisms and inflammatory parameters in lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages. Cell cultures were distributed into five groups: CTL - without ACR supplementation; LEU - supplemented with leucine (2 mmol/L); ISO - supplemented with isoleucine (2mmol / L); VAL - supplemented with valine (2 mmol/L) and LIV - supplemented with leucine (2 mmol/L), isoleucine (2 mmol/L) and valine (2 mmol/L). The inflammatory state was induced by the addition of LPS (1 µg/ml) to the culture medium, following four treatment protocols: PT - pre-treatment; TA - acute treatment; TC - chronic treatment and TT - late treatment. The cell viability assay was performed by the MTT test and the nitric oxide (NO) dosage by the Griess reaction. Pro- and anti-inflammatory cytokines, and prostaglandin E2 (PGE2) were analyzed by ELISA. For the evaluation of the molecular parameters, the western blotting method was used. There was an increase in cell viability in all supplemented groups in relation to the control group in the TA, TC and TT treatments. Regarding NO synthesis, BCAA supplementation was able to increase NO production in three of the four proposed treatments (PT, TA and TC). In relation to the production of pro- and anti-inflammatory cytokines, PT and CT were more effective in increasing this parameter, compared to the other treatments. There was no difference between groups in relation to PGE2 production and intracellular protein phosphorylation. From the obtained results it is possible to conclude that the BCAA significantly contributed to the cell viability, as well as, for the production of pro and anti-inflammatory mediators, and the supplementation protocol presents as determinant factor to obtain these results. Although the scientific literature attributed a large part of the immunomodulatory effects to leucine, the results obtained in this study attribute relevant immunomodulatory potential to isoleucine, opening space for an important study topic
Descritores: Lipopolissacarídeos
Aminoácidos de Cadeia Ramificada/efeitos adversos
Inflamação/dietoterapia
-Macrófagos/classificação
Limites: Animais
Masculino
Camundongos
Responsável: BR40.1 - DBD - Divisão de Biblioteca e Documentacão do Conjunto das Químicas


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Id: biblio-1005300
Autor: Montaño E, Dolly E; Alvarado R, Moisés G; Burgos Q, Maritza J.
Título: Validación del método de formación de coágulo para la determinación de endotoxinas bacterianas en inmunoglobulinas (IgG) nacionales / Method validation clot formation for determining bacterial endotoxins national immunoglobulin (IgG)
Fonte: Rev. Inst. Nac. Hig;47(1-2):41-48, 2016. graf, tab.
Idioma: es.
Resumo: Las endotoxinas bacterianas son lipopolisacáridos (LPS) localizados exclusivamente en la membrana externa de las bacterias gramnegativas, su ingreso al organismo a través de productos parenterales puede causar fiebre, taquicardia, aumento de presión sanguínea y en algunos casos ocasiona la muerte. Existen normativas internacionales acerca del límite de endotoxina para productos farmacéuticos inyectables, tal como las inmunoglobulinas (IgG), que pueden estar expuestas a contaminación durante el proceso de producción y por lo tanto es necesario realizar pruebas para la determinación de endotoxinas bacterianas. El método del lisado de amebocitos de Limulus (LAL) es una de ellas.Este método se fundamenta en la reacción del LAL, el cual es un extracto de células sanguíneas que interaccionan con endotoxinas, activando la cascada del proceso de coagulación y originando la formación de la coagulina. En diversas ocasiones algunas proteínas intervienen en la activación o desactivación de esta cascada, bien sea potenciando o inhibiendo la formación del coágulo. En el caso de la potenciación, el calentamiento es uno de los métodos recomendados por la farmacopea estadounidense (USP) para eliminar interferencias, puesto que desnaturaliza las proteínas que causan la potenciación sin pérdida de endotoxinas. En este trabajo se validó la determinación de endotoxinas bacterianas en IgG mediante el método LAL, el cual es un método rápido y de fácilejecución, por lo que puede implementarse como ensayo de rutina en control de calidad y por ende nos permite agilizar las Liberaciones de Lotes de estos productos.

Bacterial endotoxins are lipopolysaccharides (LPS) located exclusively in the outer membrane of gram-negative bacteria, their entry into the body through parenteral products can cause fever, tachycardia, increased blood pressure and in some cases cause death. There are international standards for endotoxin limit for injectable pharmaceuticals, such as immunoglobulins (IgG), which may be exposed to contamination during the production process and therefore it is necessary to test for determination of bacterial endotoxins. The method of the Limulus amebocyte lysate (LAL) is one of them. This method is based on the LAL reaction, which is an extract of blood cells which interact with endotoxin, triggering the cascade of the coagulation process and causing the formation of coagulin. On several occasions some proteins involved in the activation or deactivation of this waterfall, either by enhancing or inhibiting clot formation. In the case of empowerment, the warming is one of those recommended by the US Pharmacopoeia (USP) to eliminate interference, since denatures proteins that cause endotoxin enhancement lossless methods. In this paper the determination of bacterial endotoxins in IgG was standardized by the LAL method, which is quick and easy to implement method, which can be implemented as a routine test in quality control and thus allows us to streamline releases Lots of these products.
Descritores: Imunoglobulinas
Endotoxinas
Bactérias Gram-Negativas
-Lipopolissacarídeos
Células Produtoras de Anticorpos
Limites: Seres Humanos
Masculino
Feminino
Tipo de Publ: Estudos de Validação
Responsável: VE9.1 - Biblioteca


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Id: biblio-1007454
Autor: Mena, Licet; Sierra, Roxana; Valle, Maikel; Molina, Vivian; Rodriguez, Sandra; Merino, Nelson; Zamora, Zullyt; González, Victor; Medina, Jose Alberto.
Título: Acrocomia crispa fruits lipid extract prevents LPS-induced acute lung injury in mice / Extracto lipídico de los frutos de Acrocomia crispa previene el daño pulmonar agudo inducido por LPS en ratones
Fonte: Bol. latinoam. Caribe plantas med. aromát;18(1):16-26, ene. 2019. ilus, tab.
Idioma: en.
Resumo: The aim of this study was to evaluate the effects of single oral doses of D-005 (a lipid extract obtained from the fruit oil of Acrocomia crispa) on LPS-induced acute lung injury (ALI) in mice. D-005 batch composition was: lauric (35.8%), oleic (28.4%), myristic (14.2%), palmitic (8.9%), stearic (3.3%), capric (1.9%), caprylic (1.2%), and palmitoleic (0.05%) acids, for a total content of fatty acids of 93.7%. D-005 (200 mg/kg) significantly reduced lung edema (LE) (≈ 28% inhibition) and Lung Weight/Body Weight ratio (LW/BW) (75.8% inhibition). D-005 (25, 50, 100 and 200 mg/kg) produced a significant reduction of Histological score (59.9, 56.1, 53.5 and 73.3% inhibition, respectively). Dexamethasone, as the reference drug, was effective in this experimental model. In conclusion, pretreatment with single oral doses of D-005 significantly prevented the LPS-induced ALI in mice.

El objetivo de este estudio fue evaluar los efectos de dosis orales únicas de D-005 (extracto lipídico obtenido del aceite de frutos de Acrocomia crispa) sobre el daño pulmonar agudo (DPA) inducido por LPS en ratones. La composición del lote de D-005 fue: ácido láurico (35.8%), oleico (28.4%), mirístico (14.2%), palmítico (8.9%), esteárico (3.3%), cáprico (1.9%), caprílico (1.2%) y palmitoleico (0.05%), con un contenido total de ácidos grasos de 93.7%. D-005 (200 mg/kg) redujo significativamente el edema pulmonar (EP) (≈ 28% de inhibición) y la relación peso pulmón/peso corporal (PP/PC) (75.8% de inhibición). D-005 (25, 50, 100 y 200 mg/kg) produjo una reducción significativa de la puntuación histológica (59.9, 56.1, 53.5 y 73.3% de inhibición, respectivamente). La dexametasona, fármaco de referencia, fue efectiva en este modelo experimental. En conclusión, el pretratamiento con dosis orales únicas de D-005 previno significativamente el DPA inducido por LPS en ratones.
Descritores: Extratos Vegetais/administração & dosagem
Arecaceae
Lesão Pulmonar Aguda/prevenção & controle
-Extratos Vegetais/química
Lipopolissacarídeos/efeitos adversos
Administração Oral
Cromatografia Gasosa
Lesão Pulmonar Aguda/induzido quimicamente
Ácidos Graxos/análise
Frutas
Pulmão/efeitos dos fármacos
Limites: Animais
Camundongos
Responsável: CL1.1 - Biblioteca Central


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Id: biblio-996744
Autor: Ayala, Thais Soprani.
Título: A hiperglicemia altera o perfil inflamatório e imunometabólico de macrófagos derivados de medula óssea de camundongos / Hyperglycemia alters the inflammatory and immunometabolic profile of mouse bone marrow derived macrophages.
Fonte: São Paulo; s.n; 2019. 153 p. graf, ilus.
Idioma: pt.
Tese: Apresentada a Universidade de São Paulo. Faculdade de Ciências Farmacêuticas para obtenção do grau de Doutor.
Resumo: O diabetes mellitus é um grupo heterogêneo de distúrbios metabólicos caracterizado pela hiperglicemia. Indivíduos diabéticos possuem maior susceptibilidade a infecções comparado a indivíduos sadios e a hiperglicemia é um dos principais fatores que contribuem para isso, em parte, por alterar a resposta imune. Sendo assim, os macrófagos, como células essenciais para a resposta inflamatória, podem apresentar importante papel na resposta imune alterada de indivíduos diabéticos. Neste estudo, investigamos como a hiperglicemia modula os macrófagos derivados da medula óssea (BMDMs) sob um estímulo inflamatório. Para realizar este estudo, os BMDMs de camundongos C57BL/6 machos não diabéticos e diabéticos (60 mg/kg de aloxana, iv) (CEUA / FCF / USP-488) foram cultivados sob condições normais de glicose (5,5 mM) e alta concentração de glicose (25 mM ou 40 mM) e estimuladas ou não com lipopolissacarídeo (LPS, 100 ng/mL). Em comparação com os BMDMs dos camundongos não diabéticos, os BMDMs dos camundongos diabéticos estimulados com LPS apresentaram menor expressão de CD38 no tempo basal e após 24 horas, além de menor expressão de receptor do tipo Toll (TLR)-4 na superfície celular, menor capacidade fagocítica e redução na secreção de óxido nítrico, lactato, fator de necrose tumoral- e interleucina (IL)-10, porém apresentaram maior expressão de CD80, CD86 e MHC-II, maior consumo de oxigênio e maior fosforilação em quinase ativada por estresse/quinase Jun-amino-terminal (SAPK/JNK) subunidade p46 e em quinase regulada por sinal extracelular (ERK) subunidade p42, proteína quinase B (AKT) e proteína quinase C (PKC)-δ assim como maior secreção de IL-6. Quando os BMDMs dos camundongos não diabéticos foram cultivados sob condições de alta concentração de glicose in vitro e estimulados com LPS, a expressão de TLR4 e os níveis de óxido nítrico e peróxido de hidrogênio foram reduzidos. Por outro lado, os BMDMs diabéticos que também foram cultivados em alta concentração de glicose in vitro apresentaram níveis aumentados de lactato e fosforilação reduzida em AKT e PKC-δ, porém apresentaram fosforilação aumentada em p46 SAPK/JNK. A alta concentração de glicose parece modificar o comportamento dos macrófagos, afetando diferentes aspectos dos BMDMs diabéticos e não diabéticos sob estímulo de LPS, assim a hiperglicemia deixa um legado de glicose, induzindo uma memória glicêmica, alterando o estado basal dos macrófagos, modificando a via de sinalização do TLR4 contribuindo para a susceptibilidade de indivíduos diabéticos a infecções

Diabetes mellitus is a heterogeneous group of metabolic disorders characterized by hyperglycemia. Diabetic individuals are more susceptible to infections compared to healthy subjects, and hyperglycemia is one of the major contributing factors, partly because they alter the immune response. Thus, macrophages, as essential cells for the inflammatory response, may play an important role in the altered immune response of diabetic individuals. In this study, we investigated how hyperglycemia modulates bone marrow derived macrophages (BMDMs) under an inflammatory stimulus. To perform this study, BMDMs from non-diabetic male and diabetic C57BL/6 mice (60 mg / kg aloxane, iv) (CEUA / FCF / USP-488) were cultured under normal glucose conditions (5.5 mM) and high glucose concentration (25 mM or 40 mM) and stimulated or not with lipopolysaccharide (LPS, 100 ng / ml). Compared to non-diabetic mice BMDMs, the BMDMs of LPS-stimulated diabetic mice showed lower expression of CD38 at baseline and after 24 hours, as well as lower Toll-like receptor (TLR)-4 on the cell surface, lower secretion of lactate, tumor necrosis factor-, and interleukin (IL)-10, but showed higher expression of CD80, CD86 and MHC-II, higher oxygen consumption and greater phosphorylation in stress-activated kinase/Jun-amino-terminal kinase (SAPK / JNK) p46 subunit and in extracellular signal regulated kinase (ERK) p42 subunit, protein kinase B (AKT) and protein kinase C (PKC)-δ as well as higher secretion of IL-6. When the BMDMs of nondiabetic mice were cultured under conditions of in vitro high glucose concentration and stimulated with LPS, the levels of TLR4 expression, nitric oxide and hydrogen peroxide were reduced. On the other hand, diabetic BMDMs that were also cultured in high glucose concentration of glucose in vitro showed increased levels of lactate and reduced phosphorylation in AKT and PKC-δ, but showed increased phosphorylation in p46 SAPK/JNK. A high glucose concentration seems to modify the behavior of macrophages, affecting different aspects of diabetic and non-diabetic BMDMs under the same LPS stimulus. Hyperglycemia leaves a glucose legacy, inducing a glycemic memory, altering the basal state of macrophages, modifying the TLR4 signaling pathway, and may play a key role in the high susceptibility of diabetic individuals to infections
Descritores: Hiperglicemia/complicações
Inflamação/complicações
Macrófagos/metabolismo
-Lipopolissacarídeos
Diabetes Mellitus/classificação
Glucose
Limites: Animais
Masculino
Camundongos
Responsável: BR40.1 - DBD - Divisão de Biblioteca e Documentacão do Conjunto das Químicas
BR40.1; T616.462, A973h. 30100022546-F



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