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Pesquisa : D09.698.365.450 [Categoria DeCS]
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Id: biblio-1115255
Autor: Texta Nogueda, Javier; Sánchez-Santillán, Paulino; Hernández Sánchez, David; Torres-Salado, Nicolás; Crosby Galvan, María; Rojas-García, Rafael; Herrera-Pérez, Jerónimo; Maldonado-Peralta, María.
Título: Uso de disacáridos y carbón activado para preservar consorcios de bacterias ruminales celulolíticas liofilizadas / Use of disaccharides and activated carbon to preserve cellulolytic ruminal bacterial consortiums lyophilized
Fonte: Rev. MVZ Córdoba;24(3):7305-7313, sep.-dic. 2019. tab.
Idioma: es.
Resumo: RESUMEN Objetivo. Determinar la fermentación in vitro de consorcios bacterianos ruminales celulolíticos (CBC) conservados por liofilización usando carbón activado, maltosa y lactosa como preservadores. Materiales y métodos. Un CBC se aisló de fluido ruminal de una búfala de agua en medios selectivos celulolíticos. Los CBC se liofilizaron con carbón activado (CA), lactosa (LA) o maltosa (MA) como preservadores y sin preservador (SP). El diseño experimental fue completamente al azar para medir biogás a diferentes intervalos de tiempo; así como, un diseño completamente al azar con arreglo factorial 4x3, los factores fueron preservadores (SP, CA, LA y MA) y tiempo de fermentación (24, 48 y 72 h) para pH, nitrógeno amoniacal (N-NH3), degradación de materia seca (DMS) y de fibra detergente neutro (DFDN), actividad enzimática celulasas y la población de bacterias totales. Resultados. LA produjo mayor biogás acumulado a las 72 h y parcial a partir de las 12 h (p≤0.05). SP no mostró diferencias (p>0.05) en celulasas, conteo de bacterias total, DMS y DFDN en los tiempos de fermentación evaluados con el resto de los preservadores. Conclusiones. La producción de biogás parcial y acumulada, el aumento en la tasa de degradación de 8.3 y 91.1 % en la DMS y DFDN de las 24 a 72 h (p≤0.05) con el preservador LA, muestran que la lactosa puede usarse como preservador de bacterias celulolíticas ruminales.

ABSTRACT Objective. To determine in vitro fermentation of cellulolytic ruminal bacterial consortia (CBC) preserved by lyophilization using activated carbon, maltose and lactose as preservatives. Materials and methods. A CBC was isolated from the ruminal fluid of a female water buffalo in selective cellulolytic media. The CBC were lyophilized without preservative (SP), activated carbon (CA), lactose (LA) o maltose (MA) as preservatives. The experimental design was completely random to measure biogas at different time intervals; as well as completely random with 4x3 factorial arrangement, factors were preservative [SP, CA, LA and MA] and fermentation time (24, 48 and 72 h) for pH, ammoniacal nitrogen (NH3-N), dry matter degradation (DMD), neutral detergent fiber degradation (NDFD), enzymatic activity cellulases and total bacteria population. Results. LA produced higher accumulated biogas at 72 h and partial biogas after 12 h (p≤0.05). SP did not show differences (p>0.05) in cellulases, total bacteria population, DMD and NDFD in the fermentation times evaluated with the rest of the preservative. Conclusions. The production of partial and accumulated biogas, the increase in the degradation rate of 8.3 and 91.1% in the DMD and NDFD from 24 to 72 h (p≤0.05) in the LA preservative, show that lactose can be used as a preservative of ruminal cellulolytic bacteria.
Descritores: Carvão Vegetal
Dissacarídeos
Fermentação
Liofilização
-Lactose
Maltose
Limites: Animais
Tipo de Publ: Artigo Clássico
Responsável: CO140 - Facultad de Medicina Veterinária y Zootecnia


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Id: biblio-1254807
Autor: Cielecka-Piontek, Judyta; Dziedzinski, Marcin; Szczepaniak, Oskar; Kobus-Cisowska, Joanna; Telichowska, Aleksandra; Szymanowska, Daria.
Título: Survival of commercial probiotic strains and their effect on dark chocolate synbiotic snack with raspberry content during the storage and after simulated digestion
Fonte: Electron. j. biotechnol;48:62-71, nov. 2020. tab, graf.
Idioma: en.
Projeto: National Center for Research and Development in Poland.
Resumo: BACKGROUND: A key challenge for manufacturers of pro-health food containing active probiotic microorganisms is to develop a product with attractive sensory features along with maintenance of declared number of microorganisms during storage and transfer by alimentary tract. RESULTS: The highest concentration of polyphenols was observed in snacks without an additive of probiotics as well as those with an additive of L. rhamnosus and B. animalis bacteria and concentration of these compounds increased by 9.5% during six months of storage. None of the products distinguished itself in the sensorial assessment although each was assessed positively. The number of microorganisms was stable and comparatively high during six months of storage at a room temperature and in cooling conditions (108 cfu/g). In the digestion model, an influence of aggressive digestion conditions was examined in the alimentary tract on the number of microorganisms, which allowed to arrange strains from the most resistant (S. boulardii) to the most sensitive (B. breve). It must be noted that currently on the market there is no available snack containing probiotic yeast as well as there is no literature data on works on such formulation of food. CONCLUSIONS: In the newly developed snack made of chocolate, in which sugar has been replaced with maltitol, a raw material was added in the form of raspberry, prebiotic in the form of inulin and a strain of probiotic bacteria, including the unprecedented so far S. boulardii, which stands a high chance to occupy a good place on the market of functional food.
Descritores: Probióticos
Alimento Funcional
Chocolate/microbiologia
-Álcoois Açúcares
Temperatura
Alimentos Integrais
Digestão
Armazenamento de Alimentos
Prebióticos
Simbióticos
Polifenóis
Lanches
Rubus
Maltose/análogos & derivados
Responsável: CL1.1 - Biblioteca Central


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Texto completo SciELO Brasil
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Id: biblio-1132186
Autor: Souza, Patrícia Nirlane da Costa; Tavares, Dérica Gonçalves; Souza, Cláudia Regina Fernandes; Martinez, Marcelo Luis Lombardi; Oliveira, Wanderley Pereira; Guimarães, Luís Henrique Souza; Cardoso, Patrícia Gomes.
Título: Spray Drying of Coloring Extracts Produced by Fungi Isolated from Brazilian Caves
Fonte: Braz. arch. biol. technol;63:e20190024, 2020. tab, graf.
Idioma: en.
Projeto: National Council of Scientific and Technological Development; . Foundation of Research Support of Minas Gerais State; . State of São Paulo Research Foundation.
Resumo: Abstract Pigments produced by submerged fermentation of three filamentous fungi isolated from Brazilian caves, namely Aspergillus keveii, Penicillium flavigenum, and Epicoccum nigrum, were submitted to spray drying in presence of the adjuvants maltodextrin, modified starch or gum arabic. Yellow fine powders with low moisture content and water activity, and high color retention (> 70%) were successfully generated with a high product recovery ratio (> 50%), independently of the adjuvant used. The dried products have enhanced stability and potential to might be used as a natural colorant in food and pharmaceutical applications.
Descritores: Pigmentos Biológicos/biossíntese
Amido/biossíntese
Fungos/metabolismo
Goma Arábica
Maltose/biossíntese
-Aspergillus
Brasil
Cavernas/microbiologia
Fungos/classificação
Maltose/análogos & derivados
Modelos Teóricos
Limites: Animais
Responsável: BR1.1 - BIREME


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Id: lil-757858
Autor: Konar, Atheni; Sarkar, Tandra; Sukul, Nirmal Chandra; Sukul, Anirban; Chakraborty, Indrani.
Título: Transfer of the effect of potentized mercuric chloride on á-amylase fromone test tube to another through capillary water
Fonte: Int. j. high dilution res;14(1):4-11, 2015. ilus, graf.
Idioma: en.
Resumo: In a series of experiments we showed that treatment of a plant or animal with a dilutedand agitated substance might affect other plants or animals connected to the former by the capillarywater in cotton threads. The aim of the present study was to establish whether drug effect could betransferred in a cell-free medium.Design: Two test tubes, each containing 1 ml of 1% starch solution and 1 ml of á-amylase, wereconnected by means wet cotton threads encased in a polythene tube. One of the tubes alsocontained Mercurius corrosivus (Merc-c) 30 cH and the other ethanol solution (control). After 15min, the enzyme activity was stopped with DNSA, and the breakdown product of starch, maltose,was estimated. A third, separate tube contained all the tested materials except for Merc-c and thecontrol solution. In a second experiment two tubes, one containing 1,200 ppm and the other 200ppm of maltose, were similarly connected over 15 min. Both experiments were repeated 20 times.Results: In the first experiment, the amount of maltose was similar in both connected tubes, but itwas significantly lower in the unconnected tube. In the second experiment, maltose concentrationin both tubes remained unchanged.Conclusion: The information of Merc-c 30 cH was effectively transferred through capillary waterbetween two tubes in cell-free medium. This effect was not due to physical transfer of either solventor solutes. Water seems to the most probable carrier of information in diluted and agitatedsolutions...
Descritores: Altas Potências
Amido
Homeopatia
Maltose
Mercurius Corrosivus/análise
Técnicas In Vitro
alfa-Amilases
Água
-Etanol
Limites: Humanos
Responsável: BR926.1 - Biblioteca Artur de Almeida Rezende Filho


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Id: lil-771925
Autor: SOBRADO, Carlos Walter; CANÇADO, Rodolfo Delfini; SOBRADO, Lucas Faraco; FRUGIS, Marcos Onofre; SOBRADO, Marcel Faraco.
Título: TREATMENT OF ANEMIA AND IMPROVEMENT OF QUALITY OF LIFE AMONG PATIENTS WITH CROHN'S DISEASE: experience using ferric carboxymaltose
Fonte: Arq. gastroenterol;52(4):255-259, Oct.-Dec. 2015. tab.
Idioma: en.
Resumo: Objectives - Anemia is the most common hematological alteration in patients with Crohn's disease, and is frequently related to intestinal inflammatory activity. Its cause is multifactorial and mostly associated with absolute iron deficiency (iron deficiency anemia) and/or functional iron deficiency (inflammation anemia or anemia of chronic disease). It may also be identified through other causes, such as folic acid or vitamin B12 deficiency and secondary to adverse effects from medications (salicylic derivatives and immunosuppressive drugs). In the present study, patients with active Crohn's disease and anemia were evaluated and treated with intravenous ferric carboxymaltose. We discuss the therapeutic schemes (doses), safety, results and improvement of quality of life. Methods - In the present prospective study, 10 consecutive patients with Crohn's disease, with moderate to severe activity, with anemia (Hb: 6.7 to 10 g/dL), who were attended between March 2014 and March 2015, were evaluated. Six (60%) were men and four were women, all with moderate or severe anemia (hemoglobin <10 g/dL). They were treated with a maximum of three intravenous infusions of 1000 mg of ferric carboxymaltose, of at least 15 minutes in duration. It was also sought to correlate the inflammatory Crohn's disease activity degree (measured using the Crohn's Disease Activity Index, CDAI) and C-reactive protein level with the severity of anemia. The primary outcome was an increase in Hb of ≥2 g/dL and the secondary outcome was the normalization of anemia (Hb ≥12 g/dL for women and ≥13 g/dL for men) and the improvement in quality of life seen 12 weeks after the last application of carboxymaltose. Results - Among the 10 patients studied, parenteral iron supplementation was administered in three cases during hospitalization and the others received this on an outpatient basis. The total iron dose ranged from 1,000 to 2,000 mg, with an average of 1,650 mg. Crohn's disease activity measured using CDAI and C-reactive protein correlated with the intensity of anemia. An increase of 2 g/dL occurred in eight (80%) patients after 12 weeks and normalization of anemia was found in seven (70%) patients. Improvements in quality-of-life scores were found for all (100%) patients after 12 weeks. Carboxymaltose was well tolerated. Three patients presented adverse reactions (two with nausea and one with headache) of mild intensity. Conclusions - Anemia is a frequent complication for Crohn's disease patients. Intravenous iron therapy has been recommended for Crohn's disease patients, because for these patients, oral iron absorption is very limited. This is because of the inflammatory state and "blocking" of iron entry into enterocytes through hepcidin action on ferroportin, along with the elevated rates of gastrointestinal adverse events that compromise adherence to treatment and possibly aggravate the intestinal inflammatory state. The degree of Crohn's disease activity, as measured using CDAI and C-reactive protein, correlates with the severity of anemia. Carboxymaltose is a safe drug, which can be administrated in high doses (up to 1,000 mg per application per week) and corrects anemia and iron stocks over a short period of time, with consequent improvement in quality of life.

Objetivos - Anemia é a alteração hematológica mais comum em portadores de doença de Crohn, estando frequentemente relacionada à atividade inflamatória intestinal. Sua causa é multifatorial, está associada na maioria das vezes com deficiência absoluta de ferro (anemia ferropriva) e/ou deficiência funcional de ferro (anemia da inflamação ou anemia de doença crônica), podendo também ser identificada outras causas como deficiência de ácido fólico ou vitamina B12 e secundária a efeitos adversos de medicamentos (derivados salicílicos e imunossupressores). Neste trabalho, avaliamos portadores de doença de Crohn em atividade com anemia que foram tratados com carboximaltose férrica endovenosa, e discutimos os esquemas terapêuticos (doses), a segurança e os resultados, assim como a melhora da qualidade de vida. Métodos - Neste estudo prospectivo, avaliamos 10 consecutivos pacientes portadores de doença de Crohn de moderada a grave atividade com anemia (Hb: 6,7 a 10 g/dL) que foram atendidos no período de março de 2014 a março de 2015. Eram seis (60%) do sexo masculino e quatro do sexo feminino, todos com anemia moderada ou grave (hemoglobina <10g/dL), tratados com no máximo três infusões de 1000 mg de carboximaltose férrica por via endovenosa em, pelo menos, 15 minutos. Procurou-se também correlacionar o grau de atividade inflamatória da doença de Crohn (mensuração realizada com o IADC-índice de atividade da doença de Crohn) e dosagem da proteína C reativa com a gravidade da anemia. O desfecho primário foi aumento da Hb de ≥2 g/dL e desfecho secundário a normalização da anemia (Hb ≥12 g/dL para mulheres e ≥13 g/dL para homens) e melhora na qualidade de vida após 12 semanas da aplicação da última dose de carboximaltose férrica. Resultados - Dos 10 pacientes estudados, em 3 a suplementação parenteral de ferro foi realizada durante internação hospitalar, o restante em regime ambulatorial. A dose total de ferro administrada variou de 1.000 a 2.000 mg, sendo a média de 1.650 mg. A atividade da doença de Crohn mensurada pelo IADC e pelo PCR se correlacionou com a intensidade da anemia. O aumento de 2 g/dL ocorreu em oito (80%) pacientes após 12 semanas e a normalização da anemia foi observada em sete (70%). Melhora do escore de qualidade de vida foi observada em todos (100%) após 12 semanas. A carboximaltose férrica foi bem tolerada, três pacientes apresentaram reações adversas (2 - náusea e 1 - cefaléia) de leve intensidade. Conclusões - Anemia é uma complicação frequente em portadores de doença de Crohn. A terapia com ferro por via endovenosa tem sido a recomendada em portadores de doença de Crohn, pois nestes pacientes a absorção do ferro oral é bastante limitada devido ao estado inflamatório e "bloqueio" da entrada de ferro nos enterócitos por ação da hepcidina sobre a ferroportina, além das elevadas taxas de eventos adversos gastrointestinais que comprometem a adesão ao tratamento e podem agravar o estado inflamatório intestinal. O grau de atividade da doença de Crohn mensurado pelo IADC e PCR se correlaciona com a severidade da anemia. A carboximaltose férrica é uma droga segura, pode ser administrada em altas doses (até 1.000 mg por aplicação por semana), corrige a anemia e os estoques de ferro em curto espaço de tempo, com consequente melhora da qualidade de vida.
Descritores: Anemia Ferropriva/tratamento farmacológico
Doença de Crohn/complicações
Compostos Férricos/administração & dosagem
Maltose/análogos & derivados
Qualidade de Vida
-Anemia Ferropriva/etiologia
Anemia Ferropriva/psicologia
Doença de Crohn/psicologia
Maltose/administração & dosagem
Estudos Prospectivos
Índice de Gravidade de Doença
Resultado do Tratamento
Limites: Adulto
Idoso
Feminino
Humanos
Masculino
Pessoa de Meia-Idade
Adulto Jovem
Responsável: BR1.1 - BIREME


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Texto completo SciELO Brasil
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Id: lil-709863
Autor: Ribeiro Filho, José D; Farias, Sheila K; Donner, Athina C; Oliveira, Dyego P; Guimarães, José D; Souza, Maria V; Gomes, Cláudio L. N; Avanza, Marcel F. B.
Título: Efeito de soluções eletrolíticas enterais com diferentes osmolaridades sobre o perfil eletrolítico e bioquímico de equinos / Horses treated with enteral electrolyte solutions with differents osmolarities
Fonte: Pesqui. vet. bras = Braz. j. vet. res;34(2):179-184, fev. 2014. tab.
Idioma: pt.
Resumo: Foram comparados os efeitos de soluções eletrolíticas com diferentes osmolaridades administradas via enteral por sonda nasoesofágica de pequeno calibre, em fluxo contínuo, sobre o perfil bioquímico em equinos. Foram utilizadas seis fêmeas adultas em dois quadrados latinos 6x3 simultâneos em modelo misto. Os animais foram distribuídos em três grupos e cada grupo submetido aos seguintes tratamentos: HipoMalt - 5g de cloreto de sódio, 0,5g de cloreto de potássio, 0,2g de pidolato de magnésio, 1g de gluconato de cálcio e 10g de maltodextrina diluídos em 1.000mL de água (181mOsmol L-1), HipoDext - 5g de cloreto de sódio, 0,5g de cloreto de potássio, 0,2g de pidolato de magnésio, 1g de gluconato de cálcio e 10g de dextrose diluídos em 1.000mL de água (228mOsmol L-1) e IsoProp - 5g de cloreto de sódio, 0,5g de cloreto de potássio, 0,2g de pidolato de magnésio e 10g de propionato de cálcio diluídos em 1.000mL de água (282mOsm L-1). As soluções contendo dextrose (HipoDext) e maltodextrina (HipoMalt) foram mais eficazes em aumentar a taxa glicêmica sem ocasionar desequilíbrio eletrolítico. Já o tratamento com propionato de cálcio (IsoProp) além de aumentar o lactato plasmático não teve efeito sobre a glicemia.

We compared the effects of electrolyte solutions with different osmolarities administered through enteral route by naso-esophageal probe of small-caliber with continuos flow on the electrolytic and biochemical profile in horses. Six adult females were used in two simultaneous 6x3 Latin squares mixed model. The animals were divided into three groups and received the following treatments: HipoMalt - 5g of sodium chloride, 0.5g of potassium chloride, 0.2g of magnesium pidolate, 1g of calcium gluconate and 10g of maltodextrin diluted in 1.000mL of water (181mOsmol L-1); HipoDext - 5g of sodium chloride, 0.5g of potassium chloride, 0.2g of magnesium pidolate, 1g of calcium gluconate and 10g of dextrose diluted in 1.000mL of water (228mOsmol L-1); IsoProp - 5g of sodium chloride, 0.5g of potassium chloride, 0.2g of magnesium pidolate, 1g of calcium gluconate and 10g of calcium propionate diluted in 1.000mL of water (282mOsm L-1). The hypotonic electrolyte solutions containing dextrose (HipoDext) and maltodextrin (HipoMalt) were more effective in increase the rate glucose without causing electrolyte imbalance. Treatment with calcium propionate (IsoProp) besides increasing plasma lactate had no effect on blood glucose.
Descritores: Cavalos/fisiologia
Equilíbrio Hidroeletrolítico
Equilíbrio Hidroeletrolítico/fisiologia
Nutrição Enteral/veterinária
Concentração Osmolar
Soluções Hipotônicas/uso terapêutico
Soluções para Reidratação/uso terapêutico
-Glucose/administração & dosagem
Maltose
Propionatos/administração & dosagem
Limites: Animais
Feminino
Responsável: BR68.1 - Biblioteca Virginie Buff D'Ápice


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Texto completo SciELO Costa Rica
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Id: lil-688462
Autor: Solís-Ramos, Laura Yesenia; Carballo, Laura Miranda; Valdez-Melara, Marta.
Título: Establishment of cell suspension cultures of two Costa Rican Jatropha species (Euphorbiaceae) / Establecimiento de suspensiones celulares de dos especies Jatropha (Euphorbiaceae) de Costa Rica
Fonte: Rev. biol. trop;61(3):1095-1107, sep. 2013. ilus, graf, tab.
Idioma: en.
Resumo: J. curcas has been studied in different countries and some interesting agronomic, pharmacological and industrial properties have been reported. More recently, it has been considered an important alternative source for biofuel production. The objective of this study was to establish a long-term method for the maintenance of calli and cell suspension cultures of the local species J. curcas and J. gossypifolia, in order to allow future studies for novel compounds with pharmaceutical or industrial applications. For this, friable calli were successfully induced from hypocotyl segments of J. curcas and J. gossypifolia that were cultured in semisolid MS media supplemented with 1.5mg/L, and 0.5mg/L of 2,4-D, respectively. Cell suspension cultures of J. curcas were established using 1g of 35 and 60-day calli, in 50mL of liquid MS media supplied with 1.5mg/L of 2,4-D; sucrose and maltose were additionally evaluated as carbon sources. After 35 days, cell suspension cultures initiated with 35-day calli, showed greater cell growth with a maximum biomass of 194.9g/L fresh weight, 6.59g/L dry weight and 17.3% packed volume. The exponential phase ended at day 35 for cultures initiated with 35-day calli, and at day 21 for cultures initiated with 60-day calli. Higher biomass production was obtained with sucrose. Cell cultures were established with 35-day calli in MS media with the same 2,4-D concentration used for calli induction and 30g/L sucrose. This medium was considered optimum for the maintenance and growth of cell suspensions for both species, with sub-cultures every 20 days. The biotechnological potential for the production of bioactive compounds in these species for pharmacological, agricultural and industrial applications is being evaluated.

J. curcas es un importante recurso alternativo de biocombustible. Por otro lado, propiedades de interés agronómico, farmacológico e industrial han sido reportadas para esta especie. El objetivo de este estudio fue el establecimiento y mantenimiento a largo plazo de callos y cultivos celulares en suspensión de J. curcas y J. gossypifolia, con el objetivo de permitir futuros estudios para nuevos compuestos con aplicaciones farmaceúticas e industriales. Los callos friables fueron exitosamente inducidos a partir de segmentos de hipocótilos J. curcas and J. gossypifolia cultivados en medio MS semisólido suplementado con 1.5mg/L y 0.5mg/L of 2,4-D, respectivamente. Los cultivos celulares en suspensión de J. curcas fueron establecidos utilizando 1g de callos de 35 y 60 días de edad en 50mL de medio MS líquido adicionado con 1.5mg/L de 2,4-D. Después de 35 días, los cultivos en suspensión celular iniciados con callos de 35 días, mostraron mayor crecimiento celular con una biomasa máxima de 194.9g/L de peso fresco y 6.59g/L de peso seco y 17.3% de volumen empacado. La fase exponencial finalizó al día 35 en los cultivos iniciados con callos de 35 días, y al día 21 en los cultivos iniciados con callos de 60 días. Dos fuentes de carbono fueron evaluadas: sacarosa y maltosa. La producción de mayor biomasa fue obtenida con sacarosa. Los cultivos celulares se establecieron con callos de 35 días cultivados en medio MS con la misma concentración de 2,4-D utilizada para la inducción de callos y 30g/L de sacarosa. Este medio fue considerado el óptimo para el mantenimiento y crecimiento de suspensiones celulares en ambas especies con subcultivos cada 20 días. El potencial biotecnológico para la producción de compuestos bioactivos en estas especies, para aplicaciones farmacológicas, agrícolas e industriales está siendo evaluado.
Descritores: Técnicas de Cultura de Células/métodos
Jatropha/crescimento & desenvolvimento
-Biomassa
Jatropha/efeitos dos fármacos
Maltose/administração & dosagem
Suspensões
Sacarose/administração & dosagem
Fatores de Tempo
/administração & dosagem
TEMEFOS,ABBREVIATIONS AS TOPIC-DICHLOROPHENOXYACETIC ACID/administração & dosagem
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: CR1.1 - BINASSS - Biblioteca Nacional de Salud y Seguridad Social


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Texto completo SciELO Chile
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Id: lil-551883
Autor: Lee, Yong-Seok; Park, In-Hye; Yoo, Ju-Soon; Kim, Hae-Sun; Chung, Soo-Yeol; Chandra, Muni Ramanna GariSubhosh; Choi, Yong-Lark.
Título: Gene expression and characterization of 2-keto-3-deoxy-gluconate kinase, a key enzyme in the modified Entner-Doudoroff pathway of Serratia marcescens KCTC 2172
Fonte: Electron. j. biotechnol;12(3):5-6, July 2009. ilus, tab.
Idioma: en.
Resumo: We cloned 2-keto-3-deoxy-gluconate kinase (KDGK), which catalyzes the phosphorylation of 2-keto-3-deoxygluconate (KDG) to 2-keto-3-deoxy-6-phophogluconate (KDPG) from Serratia marcescens KCTC 2172. The nucleotide sequence revealed a single open reading frame containing 1,208 bp and encoding for 309 amino acids, with a molecular weight of 33,993 Da. The enzyme was purified via GST affinity chromatography. The putative KdgT binding site was detected upstream of the initial codon. The KDG kinase utilized 2-ketogluconate (KG) and KDG as substrates. The optimal temperature and pH for KDGK activity were 50ºC and 8.0, respectively.
Descritores: Gluconatos/metabolismo
Serratia marcescens/genética
Serratia marcescens/metabolismo
-Gelatinases/biossíntese
Glutationa Transferase/biossíntese
Glutationa Transferase/metabolismo
Lipase/biossíntese
Maltose/metabolismo
Responsável: CL1.1 - Biblioteca Central


  9 / 21 LILACS  
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Id: lil-420384
Autor: Laguens, M; Collura, J; Marrone, R; Diogurdi, E; Lequerica, J; Camano, M.
Título: Métodos simples de detección de deficiencias de disacaridases / A simple method to detect disaccharides deficiency
Fonte: Acta gastroenterol. latinoam;33(2):63-71, 2003.
Idioma: es.
Resumo: A simple method, easy to perform during an endoscopic procedure, fast and inexpensive, that allows detecting deficiencies in lactase, sucrase or maltase activities is presented. Briefly, method consists in placing a duodenal biopsy sample in an adequate vial containing lactose, sucrose or maltose solution during a few minutes, and then, adding a few drops of a glucose reactive from commercial origin. Presence of any enzymatic activity is demonstrated when released glucose from any of the disaccharides chosen reacts with the second reactive, turning solution to a red colour. Its utility is discussed and compared with other diagnostic methods.
Descritores: Ensaios Enzimáticos Clínicos
Dissacaridases/deficiência
Duodeno/enzimologia
Mucosa Intestinal/enzimologia
-Colorimetria
Duodenoscopia
Duodeno/patologia
Mucosa Intestinal/patologia
Lactose/deficiência
Maltose/deficiência
Sacarase/deficiência
Limites: Humanos
Masculino
Feminino
Responsável: BR1.1 - BIREME


  10 / 21 LILACS  
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Id: lil-340686
Autor: Rolim, M. F; Araujo, P. S. de; Panek, A. D; Paschoalin, V. M. F; Silva, J. T.
Título: Shared control of maltose and trehalose utilization in Candida utilis
Fonte: Braz. j. med. biol. res = Rev. bras. pesqui. méd. biol;36(7):829-837, July 2003. tab, graf.
Idioma: en.
Projeto: FAPESP; . FINEP; . PADCT.
Resumo: Trehalose biosynthesis and its hydrolysis have been extensively studied in yeast, but few reports have addressed the catabolism of exogenously supplied trehalose. Here we report the catabolism of exogenous trehalose by Candida utilis. In contrast to the biphasic growth in glucose, the growth of C. utilis in a mineral medium with trehalose as the sole carbon and energy source is aerobic and exhibits the Kluyver effect. Trehalose is transported into the cell by an inducible trehalose transporter (K M of 8 mM and V MAX of 1.8 æmol trehalose min-1 mg cell (dry weight)-1. The activity of the trehalose transporter is high in cells growing in media containing trehalose or maltose and very low or absent during the growth in glucose or glycerol. Similarly, total trehalase activity was increased from about 1.0 mU/mg protein in cells growing in glucose to 39.0 and 56.2 mU/mg protein in cells growing in maltose and trehalose, respectively. Acidic and neutral trehalase activities increased during the growth in trehalose, with neutral trehalase contributing to about 70 percent of the total activity. In addition to the increased activities of the trehalose transporter and trehalases, growth in trehalose promoted the increase in the activity of alpha-glucosidase and the maltose transporter. These results clearly indicate that maltose and trehalose promote the increase of the enzymatic activities necessary to their catabolism but are also able to stimulate each other's catabolism, as reported to occur in Escherichia coli. We show here for the first time that trehalose induces the catabolism of maltose in yeast
Descritores: Candida
Maltose
Trealase
Trealose
-Candida
Divisão Celular
Meios de Cultura
Fatores de Tempo
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME



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