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Silva, Maria do Rosário Rodrigues
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Id: biblio-842812
Autor: Camplesi Junior, Milton; Silva, Hildene Meneses; Arantes, Adriano Moraes; Costa, Carolina Rodrigues; Ataides, Fábio Silvestre; Silva, Thaisa Cristina; Reis, Maysa de Paula Costa dos; Silva, Maria do Rosário Rodrigues.
Título: Invasive fungal infection in patients with hematologic disorders in a Brazilian tertiary care hospital
Fonte: Rev. Soc. Bras. Med. Trop;50(1):80-85, Jan.-Feb. 2017. tab, graf.
Idioma: en.
Resumo: ABSTRACT INTRODUCTION: Invasive fungal infections (IFIs) are an important complication in immunocompromised individuals, particularly neutropenic patients with hematological malignancies. In this study, we aimed to verify the epidemiology and diagnosis of IFIs in patients with hematologic problems at a tertiary hospital in Goiânia-GO, Brazil. METHODS: Data from 117 patients, involving 19 cases of IFIs, were collected. The collected data included diagnosis methods, demographics, clinical characteristics, and in vitro susceptibility to different antifungal agents. Among the 19 cases, 12 were classified as proven IFI and 7 as probable invasive aspergillosis with detection of galactomannan in blood and presence of lung infiltrates in radiographic images. Logistic regression analysis showed that the proven and probable IFIs were associated with increased risk of death. Statistical analysis demonstrated that age, sex, and underlying disease were not independently associated with risk of death in IFI patients. RESULTS: Most bloodstream isolates of Candida spp. exhibited low minimum inhibitory concentrations (MICs) to all antifungal agents tested. Voriconazole and amphotericin had the lowest MICs for Aspergillus spp. and Fusarium spp., but Fusarium spp. showed the least susceptibility to all antifungals tested. Amphotericin B, fluconazole, and itraconazole were found to be inactive in vitro against Acremonium kiliense; but this fungus was sensitive to voriconazole. CONCLUSIONS: Considering the high number of IFI cases, with crude mortality rate of 6%, we could conclude that IFIs remain a common infection in patients with hematological malignancies and underdiagnosed ante mortem. Thus, IFIs should be monitored closely.
Descritores: Infecções Fúngicas Invasivas/microbiologia
Doenças Hematológicas/microbiologia
-Aspergillus/isolamento & purificação
Aspergillus/efeitos dos fármacos
Acremonium/isolamento & purificação
Acremonium/efeitos dos fármacos
Candida/isolamento & purificação
Candida/efeitos dos fármacos
Testes de Sensibilidade Microbiana
Prevalência
Sensibilidade e Especificidade
Hospedeiro Imunocomprometido
Infecções Fúngicas Invasivas/diagnóstico
Fusarium/isolamento & purificação
Fusarium/efeitos dos fármacos
Mananas/sangue
Pessoa de Meia-Idade
Antifúngicos/farmacologia
Limites: Humanos
Masculino
Feminino
Adulto
Adulto Jovem
Responsável: BR1.1 - BIREME


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Id: biblio-1041461
Autor: Cabana, Ângela Leitzke; Mendes, Josiara Furtado; Klafke, Gabriel Baracy; Brandolt, Tchana Martinez; Melo, Aryse Martins; Meireles, Mário Carlos Araújo; Xavier, Melissa Orzechowski.
Título: Can Aspergillus fumigatus conidia cause false-positive results in the galactomannan enzyme immunoassay test?
Fonte: Rev. Soc. Bras. Med. Trop;51(3):387-389, Apr.-June 2018. tab, graf.
Idioma: en.
Resumo: Abstract INTRODUCTION: Several factors can cause false-positive results in the galactomannan (GM) test; however, others remain unknown. Presently, the impact of airborne contamination by Aspergillus conidia during enzyme-linked immunosorbent assay (ELISA) remains uninvestigated. METHODS: We studied 12 A. fumigatus isolates. Fungal conidia were serially diluted and tested for GM detection using the Platelia® Aspergillus enzyme immunoassay (EIA). RESULTS: The conidia concentration required for an EIA-positive result was 4.8 × 103 (median). CONCLUSIONS: This is the first study to evaluate the impact of environmental contamination on the Platelia® Aspergillus EIA assay. Only massive contamination can interfere with GM optical readings, suggesting that environmental contamination does not cause false-positive test results.
Descritores: Aspergillus fumigatus/isolamento & purificação
Esporos Fúngicos
Ensaio de Imunoadsorção Enzimática/efeitos adversos
Reações Falso-Positivas
Mananas
-Ensaio de Imunoadsorção Enzimática/métodos
Limites: Humanos
Responsável: BR1.1 - BIREME


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Id: lil-742543
Autor: Alvarez, Eduardo.
Título: Utilidad de la prueba Aspergillus-LFD para el diagnóstico de aspergilosis: primera experiencia en Chile / Utility of Aspergillus-LFD: first experience in Chile
Fonte: Rev. chil. infectol;32(1):117-119, feb. 2015. tab.
Idioma: es.
Resumo: Introduction: Invasive fungal diseases (IFD) by filamentous fungi are a common cause of morbidity and mortality in immunocompromised patients, especially those with myeloid leukemia. In 2011 a protocol for the rapid diagnosis of IFD by filamentous fungi was implemented in Valparaiso Region. Objectives: To describe cases of IFD by filamentous fungi of the Valparaíso Region, since the implementation of rapid diagnosis and to compare results with the period 2004-2009. Materials and Method: Descriptive and prospective study conducted in two public hospitals: Carlos van Buren at Valparaiso and Gustavo Fricke at Viña del Mar. We selected patients with a diagnosis of filamentous fungal diseases considering the EORTC/MSG criteria. Demographics, underlying diseases, risk factors for EFI, galactomannan (GM) results in blood and bronchoalveolar lavage, cultures and biopsies, treatment and overall lethality rates at 30 days were registered. Results: Eighteen patients were detected, 6 with proven and 12 probable IFD. Nine were diagnosed by GM, 8 by culture and two with both methods. In cases which the agent (9/18) was isolated from Rhizopus oryzae was the most frequent. When comparing overall lethality with the period 2004-2009, there was a reduction of 47.8%, which was statistically significant. Conclusions: Compared to data previously published in the region, demographic and comorbidities of patients with IFD caused by filamentous fungi are similar, however the currently rapid diagnosis protocol has improved survival of patients and lethality experienced overall decrease.

Introducción: la enfermedad fúngica invasora (EFI) por hongos filamentosos es una causa frecuente de morbilidad y mortalidad en pacientes inmunocomprometidos, en especial en aquellos con leucemia mieloide. En el 2011 se implementó en la Región de Valparaíso un protocolo de diagnóstico rápido de la EFI por hongos filamentosos. Objetivos: describir los casos de EFI por hongos filamentosos de la Región de Valparaíso, desde la implementación del diagnóstico rápido y compararlos con el período 2004-2009. Materiales y Método: Estudio descriptivo y prospectivo realizado en los hospitales públicos Carlos van Buren de Valparaíso y Gustavo Fricke de Viña del Mar. Se seleccionaron aquellos pacientes con diagnóstico de EFI por hongos filamentosos considerando los criterios EORTC/MSG. Se obtuvieron datos demográficos, enfermedad de base, factores de riesgo para EFI, resultados de galactomanano (GM), cultivos y biopsias, tratamiento y letalidad global a 30 días. Resultados: Se identificaron 18 pacientes, seis con EFI probadas y 12 probables. Nueve fueron diagnosticados con galactomanano, ocho con cultivos y uno con los dos métodos. En los casos en que se aisló el agente (9/18), Rhizopus oryzae fue el más frecuente. Al comparar la letalidad global con la del período 2004-2009, hubo una reducción de 47,8%, la cual fue estadísticamente significativa. Conclusiones: En relación a lo publicado anteriormente en la región, se conservan las características demográficas y de co-morbilidad de los pacientes con EFI por hongos filamentosos; sin embargo, la introducción del nuevo protocolo de diagnóstico rápido se asoció a una disminución en la letalidad global.
Descritores: Aspergilose/diagnóstico
Kit de Reagentes para Diagnóstico
Aspergillus/isolamento & purificação
Cromatografia de Afinidade/instrumentação
Técnicas Imunoenzimáticas/instrumentação
Mananas/análise
-Kit de Reagentes para Diagnóstico/economia
Fatores de Tempo
Biomarcadores/sangue
Chile
Cromatografia de Afinidade/economia
Técnicas Imunoenzimáticas/economia
Sensibilidade e Especificidade
Limites: Humanos
Responsável: BR1.1 - BIREME


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Id: biblio-978072
Autor: Delama, Ignacio; Legarraga, Paulette; González, Tamara; García, Patricia; Rabagliati, Ricardo.
Título: Evaluación del Aspergillus lateral flow device para el diagnóstico de aspergilosis invasora, experiencia en un hospital universitario / Evaluation of the lateral flow Aspergillus assay for the diagnosis of invasive aspergillosis, experience in a university hospital
Fonte: Rev. chil. infectol;35(5):574-579, 2018. tab, graf.
Idioma: es.
Resumo: Resumen Introducción: El diagnóstico de aspergilosis invasora (AI) se realiza mediante criterios clínicos y microbiológicos los que incluyen marcadores séricos. Recientemente, el test inmunocromatográfico Aspergillus lateral flow device (LFD), ha sido evaluado como método para diagnóstico de AI. Objetivo: Evaluar el desempeño de este test para el diagnóstico de AI. Material y Método: Estudio transversal en que se evaluaron muestras de suero y lavado bronco-alveolar (LBA) procesadas para galactomanano provenientes de pacientes adultos con sospecha de AI, atendidos en el Hospital Clínico de Red de Salud UCCHRISTUS. Resultados: Se procesó un total de 142 muestras de 98 pacientes, correspondientes a AI probada 5,6%, AI probable 41,5%, AI posible 12,7% y ausencia de AI 40,1%. Al confrontar los resultados con las categorías diagnósticas según criterios EORTC/MSG se obtuvo una sensibilidad y especificidad de LFD para diagnóstico de AI de 70,9 y 53,5% para muestras de suero y 83,3 y 38,5% para muestras de LBA. La concordancia entre galactomanano y LFD fue de 62,4% (54,1-69,9) con un índice Kappa de 0,202 (0,03682-0,3669). Conclusiones: Aspergillus LFD presentó una adecuada sensibilidad; sin embargo, la especificidad fue baja por lo que un resultado positivo requiere ser confirmado.

Background: The incidence of invasive aspergillosis is increasing. Its diagnosis is based on clinical and microbiological criteria which include the determination of serological markers such as galactomannan. Recently, the Aspergillus lateral flow device, an inmunocromatograph assay has been described for its diagnosis. Aim: To evaluate the performance of the lateral flow device for the diagnosis of invasive aspergillosis (IA) in adult patients. Material and Method: In this cross-sectional study, frozen samples that had been previously evaluated for galactomannan from patients classified with proven/probable/possible or no AI according to the EORTC/MSG criteria were selected. Results: A total of 142 samples from 98 patients were processed, corresponding to proven AI 5.6%, probable IA 41.5%, possible IA 12.7% and no-IA 40.1%. The sensitivity and specificity of the Aspergillus lateral flow was 70.9% and 53.5% for serum samples and 83.3% and 38.5% for BAL samples. The concordance between the galactomannan and Aspergillus lateral flow was 62.4% (54.1 - 69.9) with a Kappa index of 0.202 (0.03682 - 0.3669). Conclusions: We observed a good sensitivity but low specificity, a positive result need a confirmatory test.
Descritores: Aspergilose/diagnóstico
Aspergillus/genética
Aspergillus/imunologia
DNA Fúngico/análise
Líquido da Lavagem Broncoalveolar/microbiologia
Mananas/análise
-Estudos Transversais
Cromatografia de Afinidade/métodos
Sensibilidade e Especificidade
Hospitais Universitários
Limites: Humanos
Masculino
Feminino
Adulto
Pessoa de Meia-Idade
Idoso
Responsável: CL1.1 - Biblioteca Central


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Id: biblio-828120
Autor: Garnica, Marcia; Sinhorelo, Aline; Madeira, Laura; Portugal, Rodrigo; Nucci, Marcio.
Título: Diagnostic-driven antifungal therapy in neutropenic patients using the D-index and serial serum galactomannan testing
Fonte: Braz. j. infect. dis;20(4):354-359, July-Aug. 2016. tab.
Idioma: en.
Resumo: Abstract Introduction Invasive mold disease is an important complication of patients with hematologic malignancies, and is associated with high mortality. A diagnostic-driven approach has been an alternative to the classical empiric antifungal therapy. In the present study we tested an algorithm that incorporated risk stratification using the D-index, serial serum galactomannan and computed tomographic-scan to guide the decision to start antifungal therapy in neutropenic patients. Patients and methods Between May 2010 and August 2012, patients with acute leukemia in induction remission were prospectively monitored from day 1 of chemotherapy until discharge or death with the D-index and galactomannan. Patients were stratified in low, intermediate and high risk according to the D-index and an extensive workup for invasive mold disease was performed in case of positive galactomannan (≥0.5), persistent fever, or the appearance of clinical manifestations suggestive of invasive mold disease. Results Among 29 patients, 6 (21%), 11 (38%), and 12 (41%) were classified as high, intermediate, and low risk, respectively. Workup for invasive mold disease was undertaken in 67%, 73% and 58% (p = 0.77) of patients in each risk category, respectively, and antifungal therapy was given to 67%, 54.5%, and 17% (p = 0.07). Proven or probable invasive mold disease was diagnosed in 67%, 45.5%, and in none (p = 0.007) of high, intermediate, and low risk patients, respectively. All patients survived. Conclusion A risk stratification using D-index was a useful instrument to be incorporated in invasive mold disease diagnostic approach, resulting in a more comprehensive antifungal treatment strategy, and to guide an earlier start of treatment in afebrile patients under very high risk.
Descritores: Aspergilose/tratamento farmacológico
Algoritmos
Fusariose/tratamento farmacológico
Mananas/sangue
Antifúngicos/uso terapêutico
Neutropenia/imunologia
-Aspergilose/diagnóstico
Aspergilose/imunologia
Síndromes Mielodisplásicas/imunologia
Síndromes Mielodisplásicas/microbiologia
Leucemia Mieloide Aguda/imunologia
Leucemia Mieloide Aguda/microbiologia
Tomografia Computadorizada por Raios X
Estudos Prospectivos
Sensibilidade e Especificidade
Medição de Risco
Fusariose/diagnóstico
Fusariose/imunologia
Mananas/imunologia
Neutropenia/microbiologia
Limites: Humanos
Masculino
Feminino
Adulto
Pessoa de Meia-Idade
Idoso
Adulto Jovem
Responsável: BR1.1 - BIREME


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Id: biblio-888923
Autor: Passos, Ana Isabela Morsch; Dertkigil, Rachel Polo; Ramos, Marcelo de Carvalho; Busso-Lopes, Ariane Fidelis; Tararan, Cibele; Ribeiro, Erivan Olinda; Schreiber, Angélica Zaninelli; Trabasso, Plinio; Resende, Mariangela Ribeiro; Moretti, Maria Luiza.
Título: Serum markers as an aid in the diagnosis of pulmonary fungal infections in AIDS patients
Fonte: Braz. j. infect. dis;21(6):606-612, Nov.-Dec. 2017. tab, graf.
Idioma: en.
Projeto: FAPESP.
Resumo: ABSTRACT Introduction: The etiology of pulmonary infections in HIV patients is determined by several variables including geographic region and availability of antiretroviral therapy. Materials and methods: A cross-sectional prospective study was conducted from 2012 to 2016 to evaluate the occurrence of pulmonary fungal infection in HIV-patients hospitalized due to pulmonary infections. Patients' serums were tested for (1-3)-β-D-Glugan, galactomannan, and lactate dehydrogenase. The association among the variables was analyzed by univariate and multivariate regression analysis. Results: 60 patients were included in the study. The patients were classified in three groups: Pneumocystis jirovecii pneumonia (19 patients), community-acquired pneumonia (18 patients), and other infections (23 patients). The overall mortality was 13.3%. The time since diagnosis of HIV infection was shorter in the pneumocystosis group (4.94 years; p = 0.001) than for the other two groups of patients. The multivariate analysis showed that higher (1-3)-β-D-Glucan level (mean: 241 pg/mL) and lactate dehydrogenase (mean: 762 U/L) were associated with the diagnosis of pneumocystosis. Pneumocystosis was the aids-defining illness in 11 out of 16 newly diagnosed HIV-infected patients. Conclusion: In the era of antiretroviral therapy, PJP was still the most prevalent pulmonary infection and (1-3)-β-D-Glucan and lactate dehydrogenase may be suitable markers to help diagnosing pneumocystosis in our HIV population.
Descritores: Infecções Oportunistas Relacionadas com a AIDS/diagnóstico
beta-Glucanas/sangue
L-Lactato Desidrogenase/sangue
Pneumopatias Fúngicas/diagnóstico
Mananas/sangue
-Biomarcadores/sangue
Estudos Transversais
Valor Preditivo dos Testes
Estudos Prospectivos
Análise de Regressão
Sensibilidade e Especificidade
Infecções Oportunistas Relacionadas com a AIDS/sangue
Pneumopatias Fúngicas/sangue
Limites: Humanos
Masculino
Feminino
Responsável: BR1.1 - BIREME


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Id: biblio-951618
Autor: dos Santos, Jeniffer S; Hermes, Djuli M; Pasqualotto, Alessandro C.
Título: Galactomannan use in clinical practice: providing free testing is not the full answer
Fonte: Braz. j. infect. dis;22(1):37-40, Jan.-feb. 2018. tab.
Idioma: en.
Resumo: ABSTRACT Introduction: Invasive aspergillosis is a condition associated with a high mortality rate mostly due to difficulties in performing an early diagnosis. In recent years, galactomannan detection has markedly improved the diagnosis of invasive aspergillosis, but very little is known on how physicians deal with this test in clinical practice. Methods: This cross-sectional study aimed to analyze the indications for the use of serum galactomannan in a large Brazilian hospital, between 2015 and 2016. No specific protocol was in place for GM request. We reviewed the medical records of adult (>18 years-old) patients who were tested for galactomannan due to one the following indications: screening, diagnosis, or treatment follow-up. Additional variables included demographic data, underlying diseases, presence of neutropenia, and use of previous antifungal (anti-Aspergillus) drugs. Results: The mean age of the patients was 51 years-old (sd ± 15.8), and 63.3% of patients were male. Patients with hematological malignancies accounted for 60.1% of the cases, mostly acute myeloid leukemia (19.6%). Galactomannan testing was positive in 12.2% of patients, including 1.6% of occasions in which the test was used for screening purposes, 13.2% for diagnosis, and 32.4% during follow-up. Median time for chest imaging request was two days before GM testing. Previous antifungal therapy was reported for 35.1% of patients, mostly amphotericin B (57.1%). Conclusion: The correct use of galactomannan testing is essential for an early diagnosis of invasive aspergillosis, which may improve the prognosis of the disease. We demonstrated that clinicians usually ask for galactomannan tests to confirm imaging findings in patients who frequently were on antifungal drugs, something that could be improved by medical education. We observed a low frequency of galactomannan use for preemptive antifungal therapy (25.7%), which is worrying considering the well-known beneficial use of GM testing in this scenario.
Descritores: Aspergilose/diagnóstico
Aspergilose/sangue
Mananas/sangue
-Aspergilose/tratamento farmacológico
Valores de Referência
Estudos Transversais
Valor Preditivo dos Testes
Reprodutibilidade dos Testes
Estatísticas não Paramétricas
Diagnóstico Precoce
Antifúngicos/uso terapêutico
Limites: Humanos
Masculino
Feminino
Adulto
Pessoa de Meia-Idade
Idoso
Responsável: BR1.1 - BIREME


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Id: biblio-1058074
Autor: Maldonado O, Lorena; Albuja A, Evelyn; Reyes H, Mirtha; Barros M, Manuel; Cartagena S, Claudia; Cruz Ch, Rodrigo.
Título: Aspergilosis pulmonar invasora en pacientes reumatológicos: presentación de dos casos / Invasive pulmonary aspergillosis in rheumatological patients: presentation of two cases
Fonte: Rev. chil. enferm. respir;35(3):191-198, 2019. tab, graf.
Idioma: es.
Resumo: La aspergilosis pulmonar invasora (API) es una infección causada por hongos del género Aspergillus que afecta principalmente a pacientes inmunocomprometidos y corresponde a la forma más grave de aspergilosis. Se asocia a una alta morbi-mortalidad, siendo fundamental un diagnóstico y tratamiento oportuno. Las manifestaciones clínicas son inespecíficas, por lo que un estudio adecuado es importante para el diagnóstico, principalmente en pacientes con factores de riesgo poco habituales. En la actualidad se han establecido categorías diagnósticas que consideran factores del hospedero, laboratorio micológico tradicional y biomarcadores como galactomanano. Éstos, junto a la mejor comprensión e interpretación de las imágenes tomográficas permiten ofrecer un manejo adecuado. En este artículo, se presentan dos casos clínicos de API en pacientes reumatológicos, y se discute la utilidad de los métodos diagnósticos.

Invasive pulmonary aspergillosis (IPA) is an infection caused by fungi of the genus Aspergillus that mainly affects immunocompromised patients and corresponds to the most severe form of aspergillosis. It is associated with high morbidity and mortality, and diagnosis and timely treatment are essential. Clinical manifestations are nonspecific, so an adequate study is important for diagnosis, mainly in patients with unusual risk factors. At present, diagnostic categories have been established that consider factors of the host, traditional mycological laboratory and biomarkers such as galactomannan. These, together with the better understanding and interpretation of the tomographic images, allow us to offer an adequate management. In this article, two clinical cases of API in rheumatological patients are presented, and the usefulness of the diagnostic methods is discussed.
Descritores: Doenças Reumáticas/complicações
Aspergilose Pulmonar Invasiva/complicações
Aspergilose Pulmonar Invasiva/diagnóstico
-Biomarcadores/análise
Tomografia Computadorizada por Raios X
Hospedeiro Imunocomprometido
Aspergilose Pulmonar Invasiva/tratamento farmacológico
Voriconazol/uso terapêutico
Mananas/análise
Antifúngicos/uso terapêutico
Limites: Humanos
Masculino
Feminino
Adulto
Pessoa de Meia-Idade
Tipo de Publ: Relatos de Casos
Responsável: CL1.1 - Biblioteca Central


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Id: biblio-989440
Autor: Cabana, A L; Xavier, M O; Mendes, J F; Teles, A J; Martins, A M; Silva-Filho, R P; Meireles, M C A.
Título: Applicability of the Platelia EIA® Aspergillus test for the diagnosis of aspergilosis in penguins / Aplicabilidade do Platelia EIA® Aspergillus como teste diagnostico da aspergilose em pinguins
Fonte: Braz. j. biol;79(2):169-173, Apr.-June 2019. tab, graf.
Idioma: en.
Resumo: Abstract Even today, an effective diagnostic test for aspergillosis in penguins is unknown, being the gold standard post-mortem examinations. The fungal antigen galactomannan (GM) has been used as a biomarker of disease in humans and is detected by the Platelia Aspergillus EIA (BioRad)®, a commercial kit based on the sandwich ELISA technique. It is standardized for use in neutropenic patients, however studies have demonstrated its usefulness also possible for birds. The aim of our study was to evaluate the effectiveness of Platelia Aspergillus EIA® test (BioRad-US) in the diagnosis of aspergillosis in Magellanic penguins, determining sensitivity, specificity, and positive and negative predictive values for different cut-off points. Were included in the study, blood serum samples (n = 29) Magellanic penguins in captivity that died by aspergillosis. Detection of GM was performed following manufacturer's instructions and the GM index was obtained by dividing the average value of OD of the duplicate of the clinical sample by duplicate OD of the average value of the cut-off sample provided by the kit. Through information database results were obtained for the presence of anti-Aspergillus fumigatus antibodies detected by agar gel immunodiffusion (AGID) for all serum samples. Results were analyzed using chi-square test and Kruskal-Wallis from SPSS 20.0, IBM®. ROC curve was obtained and from this, rates of sensitivity, specificity, positive and negative predictive values were also calculated based on four different cutoff points (0.5, 1.0, 1.5 and 2.0). The serum GM index did not differ between animals of the case and control group (pkw =0.097). In determining the ROC curve for serum GM detection the value of area under the curve was 0.635. From the values ​​determined by the coordinate of the curve, four different cut points (0.5, 1.0, 1.5 and 2.0) were analyzed, resulting in sensitivity rates ranging from 86.2 to 34.5% % and specificity between 87% and 26.1%. By comparing the serum GM index in group case as the presence or absence of antibodies detected by AGID was found p=0.503. The detection of GM the Platelia Aspergillus EIA® test seems is not be useful for the diagnosis of aspergillosis in naturally infected penguins.

Resumo Ainda hoje, um teste diagnóstico eficaz para aspergilose em pinguins não é conhecido, sendo o padrão-ouro os exames post-mortem. O antígeno fúngico galactomanana (GM) tem sido utilizado como biomarcador da doença em humanos, sendo detectado pelo Platelia Aspergillus EIA (BioRad)®, um kit comercial que se baseia na técnica ELISA sanduíche. É padronizado para utilização em pacientes neutropênicos, no entanto estudos tem demonstrado sua possível utilidade também para aves.O objetivo de nosso estudo foi avaliar a eficácia do teste Platelia Aspergillus EIA® (BioRad-US) no diagnóstico da aspergilose em pinguins-de-Magalhães, determinando sensibilidade, especificidade e valores preditivos positivos e negativos em diferentes pontos de corte. Foram incluídas no estudo, amostras de soro sanguíneo (n=29) de pinguins-de-Magalhães em cativeiro que vieram a óbito por aspergilose. A detecção de GM foi realizada seguindo instruções do fabricante e o índice de GM foi obtido dividindo o valor da média da DO da duplicata da amostra clínica pelo valor da média da DO da duplicata da amostra de cut-off fornecida pelo kit. Através de informações em banco de dados foram obtidos resultados sobre a presença de anticorpos anti-Aspergillus fumigatus, detectada por Imunodifusão em gel de ágar (IDGA) em todas as amostras séricas. Os resultados foram analisados utilizando-se teste de qui-quadrado e Kruskal-Wallis a partir do programa estatístico SPSS 20.0, IBM®. Curva ROC foi obtida e a partir desta, taxas de sensibilidade, especificidade, valores preditivo positivo e negativo foram igualmente calculados considerando quatro diferentes pontos de corte (0.5, 1.0, 1.5 e 2.0). O índice de GM sérica não diferiu entre os animais do grupo caso e controle (pKW = 0.097). Na determinação da curva ROC para detecção de GM sérica o valor da área sobre a curva foi de 0.635. A partir dos valores determinados pelas coordenadas da curva, quatro diferentes pontos de corte (0.5, 1.0, 1.5 e 2.0) foram analisados, resultando em taxas de sensibilidade variando de 86.2% a 34.5%, e de especificidade entre 87% e 26.1%. Ao comparar o índice de GM sérica nos animais do grupo caso quanto a presença ou não de anticorpos detectados pela IDGA foi encontrado p=0.503. A detecção de GM pelo teste Platelia Aspergillus EIA® não parece ser útil para o diagnóstico da aspergilose em pinguins naturalmente infectados.
Descritores: Aspergilose/diagnóstico
Aspergilose/veterinária
Aspergillus/isolamento & purificação
Ensaio de Imunoadsorção Enzimática/métodos
Spheniscidae/microbiologia
-Biomarcadores/análise
Mananas/análise
Limites: Animais
Responsável: BR1.1 - BIREME


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Id: lil-747042
Autor: Luna, U. V; Caramori Júnior, J. G; Corrêa, G. S. S; Kiefer, C; Souza, M. A; Vieites, F. M; Cruz, R. A. S; Assis, S. D.
Título: Mananoligossacarídeo e ß-glucano em dietas de leitões desmamados / Mannan Oligosaccharides and ß-glucan in diets for weaned piglets
Fonte: Arq. bras. med. vet. zootec;67(2):591-599, Mar-Apr/2015. tab.
Idioma: pt.
Resumo: Avaliou-se o efeito da suplementação de mananoligossacarídeo, ß-glucano e antibiótico em dietas de leitões machos castrados, durante a fase de creche (21 aos 54 dias de idade), sobre o desempenho, características morfo-histológicas da mucosa intestinal e ocorrência de diarreia. Foram utilizados 368 leitões de mesma linhagem, distribuídos em delineamento inteiramente ao acaso, com quatro tratamentos (1: 330g de mananoligossacarídeo t/ração (oriundos do núcleo), 2: 1.830g de mananoligossacarídeo t/ração (330g oriundos do núcleo + 1.550g da suplementação), 3: 330g de mananoligossacarídeo (oriundos do núcleo) + 500g de ß-glucano t/ração e 4: 330g de mananoligossacarídeo (oriundos do núcleo) + 250g de Colistina t/ração) e quatro repetições com 23 animais por unidade experimental. O desempenho foi avaliado pelo ganho de peso, consumo de ração e conversão alimentar. As características morfo-histológicas da mucosa intestinal estudadas foram altura de vilosidade, profundidade das criptas intestinais, perímetro de vilosidade e a relação altura de vilosidade:profundidade de cripta do duodeno, jejuno e íleo. A ocorrência de diarreia foi observada diariamente pela avaliação de escore fecal. A suplementação de mananoligossacarídeo, ß-glucano e antibiótico na dieta de leitões machos castrados, na fase de creche, não influenciaram o desempenho e a ocorrência de diarreia. Maior altura de vilosidade e maior profundidade de criptas no duodeno e íleo foram verificadas nos animais suplementados com ß-glucano. No jejuno foi observado maior perímetro de vilosidade nos animais suplementados com ß-glucano e nos animais que não receberam suplemento adicional na dieta.(AU)

The aim of this study was to evaluate the effect of supplementation with mannan oligosaccharides, ß-glucan and antibiotic in diets for castrated male piglets during the nursery phase (21 to 54 days of age) on performance, morpho-histological characteristics of the intestinal mucosa and occurrence of diarrhea. A total of 368 piglets of the same strain, distributed in a completely randomized design with four treatments (1- 330 g mannan oligosaccharides/t of diet (derived of nucleus); 2 - 1,830 g mannan oligosaccharides/t of diet (330g derived of nucleus + 1550g of supplementation); 3 - 330g mannan oligosaccharides (derived of nucleus) + 500g ß-glucan/t of diet; and 4 - 330g mannan oligosaccharides (derived of nucleus) + 250g colistin/t of diet) and four replications with 23 animals per experimental unit. Performance was assessed through weight gain, feed intake and feed conversion. The morpho-histological characteristics of the intestinal mucosa studied were villus height, intestinal crypt depth, villus circumference and villus height:crypt depth of duodenum, jejunum and ileum ratio. The occurrence of diarrhea was observed daily by the evaluation of fecal score. Supplementation of mannan oligosaccharides, ß-glucan and antibiotic in the diet of castrated male piglets in the nursery phase did not affect performance and occurrence of diarrhea. Greater villus height and crypt depth in the duodenum and ileum were verified in the animals supplemented with ß-glucan. In the jejunum, greater villus circumference was observed in the animals supplemented with ß-glucan and in the animals that did not receive additional supplemented diet.(AU)
Descritores: Suínos/metabolismo
Desmame
Carboidratos/administração & dosagem
Diarreia/veterinária
Intestinos/anatomia & histologia
Mananas/administração & dosagem
-Colistina
beta-Glucanas
Limites: Animais
Responsável: BR68.1 - Biblioteca Virginie Buff D'Ápice



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