Base de dados : LILACS
Pesquisa : D09.698.718 [Categoria DeCS]
Referências encontradas : 65 [refinar]
Mostrando: 1 .. 10   no formato [Detalhado]

página 1 de 7 ir para página                  

  1 / 65 LILACS  
              next record last record
seleciona
para imprimir
Fotocópia
Texto completo
Id: biblio-1253024
Autor: Canwei, Shu; Xiaoyun, Hu; Ahmed, Nauman; Shiqi, Wang; Erxun, Zhou; Meide, Liao.
Título: Fructosan form Paenibacillus kribbensis PS04 enhance disease resistance against Rhizoctonia solani and tobacco mosaic virus
Fonte: Electron. j. biotechnol;47:43-50, sept. 2020. tab, graf.
Idioma: en.
Projeto: National Key R&D Program fromthe Ministry of Science of China; . Science and Technology Plan Project of Guangzhou, China.
Resumo: BACKGROUND: Rice sheath blight (caused by Rhizoctonia solani) and tobacco mosaic virus are very important plant diseases, causing a huge loss in global crop production. Paenibacillus kribbensis PS04 is a broad-spectrum biocontrol agent, used for controlling these diseases. Previously, extracellular polysaccharides (EPS) from P. kribbensis PS04 had been purified and their structure was inferred to be fructosan. This study aimed to evaluate the effects of exogenous EPS treatment on plant­pathogen interactions. RESULTS: Plant defense genes such as phenylalanine ammonia-lyase, catalase, chitinase, allene oxide synthase, and PR1a proteins were significantly induced by exogenous EPS treatment. Moreover, subsequent challenge of EPSpretreated plants with the pathogens (R. solani or tobacco mosaic virus) resulted in higher expression of defenseassociated genes. Increased activities of defense-associated enzymes, total phenols, and flavonoids were also observed in EPS pretreated plants. The contents of malondialdehyde in plants, which act as indicator of lipid peroxidation, were reduced by EPS treatment. CONCLUSIONS: This study comprehensively showed that EPS produced from P. kribbensis PS04 enhances disease resistance in plants by the activation of defense-associated genes as well as through the enhancement of activities of defense-related enzymes.
Descritores: Doenças das Plantas/imunologia
Rhizoctonia/patogenicidade
Vírus do Mosaico do Tabaco/patogenicidade
Paenibacillus/imunologia
-Doenças das Plantas/microbiologia
Polissacarídeos Bacterianos
Controle Biológico de Vetores
Interações Hospedeiro-Patógeno
Paenibacillus/genética
Resistência à Doença/genética
Reação em Cadeia da Polimerase em Tempo Real
Frutose/análogos & derivados
Responsável: CL1.1 - Biblioteca Central


  2 / 65 LILACS  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo SciELO Brasil
Texto completo
Id: biblio-1132173
Autor: Silveira, Marcelle Moura; Conceição, Fabricio Rochedo; Mendonça, Marcelo; Moreira, Gustavo Marçal Schmidt Garcia; Cunha, Carlos Eduardo Pouey da; Rizzi, Caroline; Hartwig, Daiane Drawanz; Moreira, Angelita da Silveira; Vendrusculo, Claire Tondo; Moreira, Ângela Nunes.
Título: Biopolymer Xanthan: A New Adjuvant for DNA Vaccines
Fonte: Braz. arch. biol. technol;63:e20190090, 2020. graf.
Idioma: en.
Resumo: Abstract DNA vaccines have been evaluated as an option to prevent several diseases. In this study, the capacity of the xanthan biopolymer to improve the DNA vaccines immune response, administered intramuscularly, was evaluated. The experimental vaccines consisted of genes encoding fragments of the proteins LigA and LigB of Leptospira interrogans serogroup Icterohaemorrhagiae serovar Copenhageni strain Fiocruz L1-130. The humoral immune response was evaluated by indirect ELISA. Cytokine expression levels were determined by RT-qPCR. Compared to the control group, the IgG antibody levels of animals immunized with pTARGET/ligAni and pTARGET/ligBrep plasmids associated with xanthan biopolymer were significantly higher than the control group. Additionally, there was a significant increase in IL-17 expression in animals vaccinated with pTARGET/ligBrep and xanthan.
Descritores: Polissacarídeos Bacterianos
DNA Recombinante/farmacologia
Adjuvantes Imunológicos/farmacologia
Xanthomonas campestris
Vacinas de DNA/farmacologia
-Biopolímeros/farmacologia
Ensaio de Imunoadsorção Enzimática
Leptospira interrogans serovar icterohaemorrhagiae
Anticorpos
Limites: Animais
Feminino
Camundongos
Responsável: BR1.1 - BIREME


  3 / 65 LILACS  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo SciELO Brasil
Texto completo
Id: biblio-1132244
Autor: Lima, Maria de Morais; Carneiro, Lucia Cesar; Machado, Mírian Ribeiro Galvão; Dias, Alvaro Renato Guerra; Zavareze, Elessandra da Rosa; Prentice, Carlos; Moreira, Angelita da Silveira.
Título: Application of Films Based on Chitosan and Xanthan Gum in Refrigerated Fish Conservation
Fonte: Braz. arch. biol. technol;63:e20190046, 2020. tab, graf.
Idioma: en.
Resumo: Abstract This research aims to determine the efficiency of chitosan and xanthan gum films in conservation of croaker fillets kept in refrigeration for 9 days. Proximal composition, loss of mass, color, pH, TVB-N (Total Volatile Bases) and microbiological profile were assessed. The films were prepared with chitosan and xanthan gum in varying mass proportions 100:0, m:m (C100XG0); 60:40, m:m (C60XG40); 50:50, m:m (C50XG50). They presented the respective values for moisture content, water solubility, thickness and water vapor permeability: 24.59%, 19.50%, 0.086 mm and 11.45gm-1.s-1.Pa-1for C100XG0; 24.58%; 20.27%, 0.091 mm and 10.41 gm-1.s-1.Pa-1for C60XG40; 22.11%, 22.06%, 0.089 mm and 10.68 gm-1.s-1.Pa-1 forC50XG50.The films were made in small bags format capable to hold about 20 g of fish fillets. A control sample was prepared in parallel, using polyethylene bags under the same storage conditions. The results showed that the chitosan films combined with xanthan gum had excellent antimicrobial properties, capable of preserving the quality of chilled fish fillets during the studied period, since it inhibited the growth of Staphylococcus coagulase-positive, Salmonella spp and coliforms at 45 ° C. Mass loss of the croaker fillets was not significantly affected by xanthan gum addition to the films. On the other hand, xanthan gum addition affected pH and color parameters of the corvina fillets. It was also verified that the combination of these two polymers promoted the reduction of N-BVT, being the C50XG50 film that presented the best response.
Descritores: Xanthomonas/química
Embalagem de Alimentos/métodos
Quitosana/química
Peixes/microbiologia
Conservação de Alimentos/métodos
-Polissacarídeos Bacterianos/química
Anti-Infecciosos
Limites: Animais
Responsável: BR1.1 - BIREME


  4 / 65 LILACS  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
Id: biblio-1116432
Autor: Demirhan, Tanju; Celik, Gokcen Yuvali; Katircioglu, Hikmet; Onbasli, Dilsad.
Título: Evaluation of anti-biofouling potential of Viburnum opulus extracts / Evaluación del potencial anti bioincrustante de extractos de Viburnum opulus
Fonte: Bol. latinoam. Caribe plantas med. aromát;19(3):321-333, mayo 2020. ilus, tab.
Idioma: en.
Projeto: Erciyes University Scientific Research Projects Unit.
Resumo: In this study the in vitro investigation of the inhibitory effect of ethanol extract of Viburnum opulus L. bark sample on Streptococcus mutans planctonic cells and biofilm has been intended. A Scanning electron microscopy analysis has been performed in order to investigate the inhibitory effect of the extract on Streptococcus mutans biofilms. Furthermore, the Exopolysaccharide and dextran production of this bacteria have been identified in the presence of the extract. It has been found out that the bark extract with the concentration of 2,5 mg/mL is able to inhibit more than 50% of the cells in the different times development phases. According to this, the exopolymeric matrix on the biofilm surface disperses and the Exopolysaccharide and dextran production get lowered in the presence of bark extract compared to the control group. It is considered that this extract can be used as an alternative approach for the new chemotherapeutic strategies against tooth decay.

En este estudio se investigó el efecto inhibitorio in vitro del extracto de etanólico de una muestra de corteza de Viburnum opulus L. en biopelículas de células planctónicas de Streptococcus mutans. Se realizó un análisis de microscopía electrónica de barrido para investigar el efecto inhibitorio del extracto sobre las biopelículas de Streptococcus mutans. Además, se identificó la producción de exopolisacárido y dextrano de esta bacteria en presencia del extracto. Se descubrió que el extracto de corteza con una concentración de 2,5 mg/ml inhibió más del 50% de las células en las diferentes fases de desarrollo. Consecuentemente, la matriz exopolimérica en la superficie de la biopelícula se dispersa y la producción de exopolisacárido y dextrano se reduce en presencia de extracto de corteza en comparación con el grupo de control. Se sugiere que este extracto puede ser usado como un enfoque alternativo para las nuevas estrategias quimioterapéuticas contra la carie dental.
Descritores: Streptococcus mutans/efeitos dos fármacos
Extratos Vegetais/farmacologia
Viburnum opulus/farmacologia
Viburnum/química
-Polissacarídeos Bacterianos/análise
Streptococcus mutans/metabolismo
Técnicas In Vitro
Microscopia Eletrônica de Varredura
Dextranos/análise
Biofilmes/efeitos dos fármacos
Etanol
Incrustação Biológica
Responsável: CL1.1 - Biblioteca Central


  5 / 65 LILACS  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo SciELO Brasil
Texto completo
Id: biblio-886795
Autor: XU, CHUNPING; YU, JINWEI; ZHAO, SHANSHAN; WU, SHUNGSHUNG; HE, PEIXIN; JIA, XUEWEI; LIU, YANQI; MAO, DUOBIN.
Título: Effect of carbon source on production, characterization and bioactivity of exopolysaccharide produced by Phellinus vaninii Ljup
Fonte: An. acad. bras. ciênc;89(3,supl):2033-2041, 2017. tab, graf.
Idioma: en.
Projeto: National Science Foundation of China.
Resumo: ABSTRACT The effect on different three carbon source (i.e. glucose, fructose and sucrose) on production, chemical characterization and antioxidant activity of exopolysaccharide (EPS) produced by Phellinus vaninii Ljup was investigated in this study. Amongst carbon sources examined, glucose and sucrose were favorable for the mycelia growth, while the maximum EPS yield was achieved when sucrose was employed. The predominant carbohydrate compositions in EPSs identified were gluconic acid, glucose, mannose and galactose acid. Then, FT-IR spectral analysis revealed prominent characteristic groups in EPSs. EPSs molecule exist as nearly globular shape form in aqueous solution. The variation also affects antioxidant activities by investigated by using hydroxyl and DPPH radical scavenging assay. Sucrose was best carbon source from the viewpoint of antioxidant activity due to the relatively high contents of galactose in the EPS with moderate molecular weight and polydispersity.
Descritores: Polissacarídeos Bacterianos/metabolismo
Carbono/metabolismo
Polissacarídeos Fúngicos
-Sacarose/metabolismo
Espectroscopia de Infravermelho com Transformada de Fourier
Frutose/metabolismo
Glucose/metabolismo
Responsável: BR1.1 - BIREME


  6 / 65 LILACS  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo SciELO Brasil
Texto completo
Id: lil-107772
Autor: Calvo, B; Fischman, O; Castelo Filho, A; Reis Filho, J; Del Bianco, R; Barbosa, R.M; Zaror, L.
Título: Deteccion de antigeno del polisacarido capsular de Cryptococcus neoformans en pacientes con SIDA y neurocriptococosis en Sao Paulo, Brasil / Detection of capsular polysaccharide antigen of Cryptococcus neoformans in patients with AIDS and neurocryptococcosis in Sao Paulo, Brazil
Fonte: Rev. Inst. Med. Trop. Säo Paulo;33(6):485-90, nov.-dez. 1991. tab.
Idioma: es.
Resumo: Antigeno del polisacarido capsular (AgPC) de Cryptococcus neoformans fue detectado por la tecnica de aglutinacion de latex (AL) en LCR y suero de pacientes con Sindrome de Inmunodeficiencia Adquirida (SIDA) y primer episodio de neurocriptococosis, usando como patron el examen micologico (examen directo y cultivo) de LCR. Se obtuvo una sensibilidad de 100 por ciento de AL para detectar AgPC de C. neoformans, el cual por su rapidez permite tratamiento especifico precoz. Titulos iniciales de AgPC de la levadura en esos pacientes pueden ser>1.000.000, pareciendo que cuando esos titulos estan presentes en suero, se relacionan con mortalidad durante el tratamiento. En los pacientes que sobrevivieron se observo que el examen micologico directo y AgPC de C. neoformans, en LCR y suero, permanecen positivos aun despues de tratamiento y mejoria clinica del paciente.
Descritores: Polissacarídeos Bacterianos/imunologia
Encefalopatias/imunologia
Criptococose/imunologia
Cryptococcus neoformans/imunologia
Antígenos de Bactérias/isolamento & purificação
Síndrome de Imunodeficiência Adquirida/imunologia
-Encefalopatias/líquido cefalorraquidiano
Encefalopatias/mortalidade
Encefalopatias/sangue
Criptococose/líquido cefalorraquidiano
Criptococose/mortalidade
Criptococose/sangue
Antígenos de Bactérias/líquido cefalorraquidiano
Antígenos de Bactérias/sangue
Síndrome de Imunodeficiência Adquirida/sangue
Síndrome de Imunodeficiência Adquirida/líquido cefalorraquidiano
Síndrome de Imunodeficiência Adquirida/mortalidade
Limites: Adulto
Masculino
Feminino
Adolescente
Pessoa de Meia-Idade
Humanos
Responsável: BR66.1 - Divisão de Biblioteca e Documentação


  7 / 65 LILACS  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo SciELO Brasil
Ramos, Júlia Baruque
Raw, Isaias
Texto completo
Id: lil-344561
Autor: Paz, Marcelo Fossa da; Ramos, Júlia Baruque; Hiss, Haroldo; Vicentin, Márcio Alberto; Leal, Maria Betania Batista; Raw, Isaías.
Título: Polysaccharide production in batch process of Neisseria meningitidis serogroup C comparing Frantz, modified Frantz and Cartlin 6 cultivation media
Fonte: Braz. j. microbiol;34(1):27-32, Jan.-Apr. 2003. tab, graf.
Idioma: en.
Resumo: Polysaccharide of N. meningitidis serogroup C constitutes the antigen for the vaccine against meningitis. The goal of this work was to compare three cultivation media for production of this polysaccharide: Frantz, modified Frantz medium (with replacement of glucose by glycerol), and Catlin 6 (a synthetic medium with glucose). The comparative criteria were based on the final polysaccharide concentrations and the yield coefficient cell/polysaccharide (Y P/X). The kinetic parameters: pH, substrate consumption and cell growth were also determined. For this purpose, 9 cultivation runs were carried out in a 80 L New Brunswick bioreactor, under the following conditions: 42 L of culture medium, temperature 35°C, air flow 5 L/min, agitation frequency 120 rpm and vessel pressure 6 psi, without dissolved oxygen or pH controls. The cultivation runs were divided in three groups, with 3 repetitions each. The cultivation using the Frantz medium presented the best results: average of final polysaccharide concentration = 0.134 g/L and Y P/X=0.121, followed by Catlin 6 medium, with results of 0.095 g/L and 0.067 respectively. Considering the principal advantages in the use of the synthetic medium, i.e. facilitation of a cultivation and purification steps of the polysaccharide production process, there is a possibility that in the near future, Catlin 6 will replace the traditional Frantz medium.
Descritores: Técnicas In Vitro
Neisseria meningitidis Sorogrupo C/genética
Neisseria meningitidis Sorogrupo C/isolamento & purificação
Neisseria meningitidis Sorogrupo C/patogenicidade
Polissacarídeos Bacterianos/análise
Polissacarídeos Bacterianos/isolamento & purificação
Vacinas Meningocócicas/isolamento & purificação
-Meios de Cultura
Tipo de Publ: Revisão de Integridade Científica
Estudo Comparativo
Responsável: BR32.1 - Serviço de Biblioteca e Informação Biomédica


  8 / 65 LILACS  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo SciELO Brasil
Raw, Isaias
Texto completo
Id: lil-314803
Autor: Ramos-Buraque, Júlia; Hiss, Haroldo; Vicentim, Márcio Alberto; Paz, Marcelo Fossa da; Peixoto, Argélia; Leal, Maria Betania Batista; Sato, Rony Akio; Vassoler, Umbelina Macedo; Raw, Isaías.
Título: Nitrogen consumption during batch cultivation of Neisseria meningitidis (serogroup c) in Frantz medium
Fonte: Braz. j. microbiol;32(4):305-310, Oct.-Dec. 2001. tab, graf.
Idioma: en.
Resumo: Capsular polysaccharide, extracted from microorganism cultivations, is the principal antigen for elaboration of vaccine against the disease caused by Neisseria meningitidis serogroup C. The final protein content allowed in this vaccine is 1 (per cent). In order to find a relationship between nitrogen consumption and cell growth, including polysaccharide production, and cell nitrogen content, cultivations were carried out in an 80 liters bioreactor (total capacity), under the following conditions: Frantz medium; temperature of 35ºC; air flow of 5L/min (0.125vvm); agitation frequency of 120 rpm and vessel pressure of 6 psi (k(L)a=0.07min(-1).Concentrations of biomass, total polysaccharide, cellular nitrogen, residual organic and inorganic nitrogen in the medium were measured during cultivation. From five cultivations carried out under the same conditions, a mean cell nitrogen percentage of 12.6(per cent)(w/w) in respect to the dry biomass was found. The inorganic nitrogen in the medium did not change significantly along the cultivation time, whereas the organic nitrogen consuption was linearly related to cell growth, with constant yeield factors (average of 8.44). Polysaccharide production kinetics followed the cell growth kinetics until the beginning of the stationary growth phase. A supplemental polysaccharide production was observed until the end of cultivation, but without cell nitrogen absorption. Thus, the results indicate that polysaccharide is produced in two phases, being the first one biomass formation followed by non-associated to growth. /
Descritores: Polissacarídeos
Polissacarídeos Bacterianos
Vacinas Bacterianas
Neisseria meningitidis
Técnicas In Vitro
Proteínas de Bactérias/análise
Proteínas de Bactérias/isolamento & purificação
-Meios de Cultura
Testes Sorológicos/métodos
Tipo de Publ: Revisão de Integridade Científica
Responsável: BR32.1 - Serviço de Biblioteca e Informação Biomédica


  9 / 65 LILACS  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo SciELO Brasil
Texto completo
Id: biblio-949378
Autor: Maia, Guilherme Tavares da Silva; Albuquerque, Amanda Vasconcelos de; Martins Filho, Euclides Dias; Lira Neto, Filipe Tenório de; Souza, Veridiana Sales Barbosa de; Silva, Anderson Arnaldo da; Lira, Mariana Montenegro de Melo; Lima, Salvador Vilar Correia.
Título: Bacterial cellulose to reinforce urethrovesical anastomosis. A translational study
Fonte: Acta cir. bras;33(8):673-683, Aug. 2018. tab, graf.
Idioma: en.
Resumo: Abstract Purpose: To evaluate the efficacy of the cellulosic exopolysaccharide membrane (CEM) as a urethral reinforcement for urethrovesical anastomosis. Methods: Twenty eight rabbits were submitted to urethrovesical anastomosis with or without CEM reinforcement. The animals were divided into 4 groups: C7, CEM7, C14 and CEM14: (C= only anastomosis or CEM = anastomosis + CEM), evaluated after 7 weeks, and 14 weeks. The biointegration and biocompatibility of CEM were evaluated according to stenosis, fistula, urethral wall thickness, urethral epithelium, rate of inflammation and vascularization. Results: Between the two experimental groups, the difference in the number of stenosis or urinary fistula was not statistically significant. The morphometric analysis revealed preservation of urethral lumen, well adhered CEM without extrusion, a controlled inflammatory process and implant vascularization. The urothelium height remained constant over time after CEM reinforcement and the membrane wall was thicker, statistically, after 14 weeks. Conclusion: The absence of extrusion, stenosis or urinary fistula after 14 weeks of urethrovesical anastomosis demonstrates cellulosic exopolysaccharide membrane biocompatibility and biointegration with tendency to a thicker wall.
Descritores: Uretra/cirurgia
Materiais Biocompatíveis/uso terapêutico
Bexiga Urinária/cirurgia
Celulose/uso terapêutico
-Polissacarídeos Bacterianos/uso terapêutico
Fatores de Tempo
Uretra/patologia
Bexiga Urinária/patologia
Microbiologia Industrial/métodos
Teste de Materiais
Anastomose Cirúrgica
Celulose/biossíntese
Reprodutibilidade dos Testes
Resultado do Tratamento
Pesquisa Médica Translacional
Neovascularização Patológica
Limites: Animais
Masculino
Coelhos
Tipo de Publ: Estudo de Avaliação
Responsável: BR1.1 - BIREME


  10 / 65 LILACS  
              first record previous record
seleciona
para imprimir
Fotocópia
Texto completo SciELO Brasil
Texto completo
Id: biblio-951792
Autor: Moghannem, Saad A. M; Farag, Mohamed M. S; Shehab, Amr M; Azab, Mohamed S.
Título: Exopolysaccharide production from Bacillus velezensis KY471306 using statistical experimental design
Fonte: Braz. j. microbiol;49(3):452-462, July-Sept. 2018. tab, graf.
Idioma: en.
Resumo: Abstract Exopolysaccharide (EPS) biopolymers produced by microorganisms play a crucial role in the environment such as health and bio-nanotechnology sectors, gelling agents in food and cosmetic industries in addition to bio-flocculants in the environmental sector as they are degradable, nontoxic. This study focuses on the improvement of EPS production through manipulation of different culture and environmental conditions using response surface methodology (RSM). Plackett-Burman design indicated that; molasses, yeast extract and incubation temperature are the most effective parameters. Box-Behnken RSM indicated that; the optimum concentration for each parameter was 12% (w/v) for molasses, 6 g/L yeast extract and 30 °C for incubation temperature. The most potent bacterial isolate was identified as Bacillus velezensis KY498625. After production, EPS was extracted, purified using DEAE-cellulose, identified using Fourier transform infrared (FTIR), gel permeation chromatography (GPC) and gas chromatography-mass spectroscopy (GC-MS). The result indicated that; it has molecular weight 1.14 × 105 D consisting of glucose, mannose and galactose.
Descritores: Polissacarídeos Bacterianos/metabolismo
Bacillus/metabolismo
-Polissacarídeos Bacterianos/isolamento & purificação
Polissacarídeos Bacterianos/química
Bacillus/química
Microbiologia Industrial
Espectroscopia de Infravermelho com Transformada de Fourier
Meios de Cultura/metabolismo
Meios de Cultura/química
Peso Molecular
Responsável: BR1.1 - BIREME



página 1 de 7 ir para página                  
   


Refinar a pesquisa
  Base de dados : Formulário avançado   

    Pesquisar no campo  
1  
2
3
 
           



Search engine: iAH v2.6 powered by WWWISIS

BIREME/OPAS/OMS - Centro Latino-Americano e do Caribe de Informação em Ciências da Saúde