Base de dados : LILACS
Pesquisa : D09.698.718.450.600 [Categoria DeCS]
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Texto completo SciELO Brasil
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Id: lil-748251
Autor: Benevides-Matos, Najla; Pieri, Fabio A.; Penatti, Marilene; Orlandi, Patrícia P..
Título: Adherence and virulence genes of Escherichia coli from children diarrhoea in the Brazilian Amazon
Fonte: Braz. j. microbiol;46(1):131-137, 05/2015. tab, graf.
Idioma: en.
Resumo: The bacterial pathogen most commonly associated with endemic forms of childhood diarrhoea is Escherichia coli. Studies of epidemiological characteristics of HEp-2 cell-adherent E. coli in diarrhoeal disease are required, particularly in developing countries. The aim of this study was evaluate the presence and significance of adherent Escherichia coli from diarrhoeal disease in children. The prevalence of LA, AA, and DA adherence patterns were determined in HEp-2 cells, the presence of virulence genes and the presence of the O serogroups in samples obtained from 470 children with acute diarrhoea and 407 controls in Porto Velho, Rondônia, Brazil. E. coli isolates were identified by PCR specific for groups of adherent E. coli. Out of 1,156 isolates obtained, 128 (11.0%) were positive for eae genes corresponding to EPEC, however only 38 (29.6%) of these amplified bfpA gene. EAEC were isolated from 164 (14.1%) samples; of those 41(25%), 32 (19%) and 16 (9.7%) amplified eagg, aggA or aafA genes, respectively and aggA was significantly associated with diarrhoea (P = 0.00006). DAEC identified by their adhesion pattern and there were few isolates. In conclusion, EAEC was the main cause of diarrhoea in children, especially when the aggA gene was present, followed by EPEC and with a negligible presence of DAEC.
Descritores: Diarreia/microbiologia
Escherichia coli Enteropatogênica/genética
Infecções por Escherichia coli/microbiologia
Escherichia coli/genética
Fatores de Virulência/genética
-Aderência Bacteriana
Brasil
Escherichia coli Enteropatogênica/classificação
Escherichia coli Enteropatogênica/isolamento & purificação
Escherichia coli Enteropatogênica/fisiologia
Células Epiteliais/microbiologia
Escherichia coli/classificação
Escherichia coli/isolamento & purificação
Escherichia coli/fisiologia
Antígenos O/análise
Sorogrupo
Limites: Criança
Pré-Escolar
Humanos
Lactente
Recém-Nascido
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


  2 / 6 LILACS  
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Id: lil-634557
Autor: Serafino, J.; Conde, S.; Zabal, O.; Samartino, L..
Título: Multiplicación de Brucella abortus y producción de óxido nítrico en dos líneas celulares de macrófagos de distinto origen / Multiplication of Brucella abortus and production of nitric oxide in two macrophage cell lines of different origin
Fonte: Rev. argent. microbiol;39(4):193-198, oct.-dic. 2007. ilus, tab.
Idioma: es.
Projeto: INTA. PICT N° 08-12221.
Resumo: Brucella abortus es una bacteria que causa abortos e infertilidad en el ganado y fiebre ondulante en el hombre. Se multiplica en el citoplasma celular evadiendo los mecanismos de muerte intracelular. El óxido nítrico (NO) es importante en la regulación de la respuesta inmune. En el presente trabajo estudiamos la habilidad de tres cepas de B. abortus para sobrevivir intracelularmente en dos líneas celulares de macrófagos. La multiplicación de bacterias en ambas líneas celulares fue determinada a distintos tiempos en número de UFC/ml, también fue observada al microscopio de campo claro y de fluorescencia utilizando Giemsa y naranja de acridina, respectivamente. La tinción de ambas líneas celulares inoculadas con B. abortus mostró un resultado concordante con el encontrado en la determinación del número de UFC. Fue confirmada la presencia de B. abortus por microscopía electrónica. Para medir la producción de NO se utilizó el reactivo de Griess. La multiplicación de la cepa rugosa RB51 disminuyó en ambas líneas celulares y los niveles de NO fueron mayores en células inoculadas con dicha cepa que cuando fueron inoculadas con las cepas lisas (S19 y 2308). Estos resultados sugieren que probablemente la ausencia de cadena O en el lipopolisacárido afecta el crecimiento intracelular de B. abortus.

Brucella abortus is a bacterium which causes abortions and infertility in cattle and undulant fever in humans. It multiplies intracellularly, evading the mechanisms of cellular death. Nitric oxide (NO) is important in the regulation of the immune response. In the present work, we studied the ability of three B. abortus strains to survive intracellularly in two macrophage cell lines. The bacterial multiplication in both cell lines was determined at two different times in UFC/ ml units. Moreover the inoculated cells were also observed under light-field and fluorescence microscopy stained with Giemsa and acridine orange, respectively. The stain of both cellular lines showed similar results with respect to the UFC/ml determination. The presence of B. abortus was confirmed by electronic microscopy. In both macrophage cell lines inoculated with RB51, the multiplication diminished and the level of NO was higher, compared with cells inoculated with smooth strains (S19 and 2308). These results suggest that the absence of O-chain of LPS probably has affects the intracellular growth of B. abortus.
Descritores: Cápsulas Bacterianas/fisiologia
Brucella abortus/crescimento & desenvolvimento
Macrófagos/microbiologia
Óxido Nítrico/biossíntese
-Cápsulas Bacterianas/química
Brucella abortus/classificação
Brucella abortus/metabolismo
Brucella abortus/ultraestrutura
Divisão Celular
Linhagem Celular/metabolismo
Linhagem Celular/microbiologia
Macrófagos/metabolismo
Macrófagos Peritoneais/metabolismo
Macrófagos Peritoneais/microbiologia
Microscopia Eletrônica
Antígenos O/fisiologia
Especificidade da Espécie
Limites: Animais
Bovinos
Camundongos
Tipo de Publ: Estudo Comparativo
Research Support, Non-U.S. Gov't
Responsável: AR1.2 - Instituto de Investigaciónes Epidemiológicas


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Texto completo SciELO Chile
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Id: lil-626743
Autor: Hoare, Anilei; Bravo, Denisse; Martinic, Mara; Valvano, Miguel A; Contreras, Inés; Álvarez, Sergio A.
Título: The normal chain length distribution of the O antigen is required for the interaction of Shigella flexneri 2a with polarized Caco-2 cells
Fonte: Biol. Res;45(1):21-26, 2012. ilus, tab.
Idioma: en.
Projeto: CONICYT/World Bank; . Canadian Institutes of Health Research.
Resumo: Shigella flexneri causes bacillary dysentery in humans. Essential to the establishment of the disease is the invasion of the colonic epithelial cells. Here we investigated the role of the lipopolysaccharide (LPS) O antigen in the ability of S. flexneri to adhere to and invade polarized Caco-2 cells. The S. flexneri 2a O antigen has two preferred chain lengths: a short O antigen (S-OAg) regulated by the WzzB protein and a very long O antigen (VL-OAg) regulated by Wzz pHS2. Mutants with defined deletions of the genes required for O-antigen assembly and polymerization were constructed and assayed for their abilities to adhere to and enter cultured epithelial cells. The results show that both VL- and S-OAg are required for invasion through the basolateral cell membrane. In contrast, the absence of O antigen does not impair adhesion. Purified LPS does not act as a competitor for the invasion of Caco-2 cells by the wild-type strain, suggesting that LPS is not directly involved in the internalization process by epithelial cells.
Descritores: Aderência Bacteriana/fisiologia
Proteínas de Bactérias/análise
Disenteria Bacilar/microbiologia
Antígenos O/química
Shigella flexneri/patogenicidade
-CACO-TEMEFOS CELLS
Disenteria Bacilar/imunologia
Antígenos O/metabolismo
Polimerização
Shigella flexneri/imunologia
Limites: Humanos
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: CL1.1 - Biblioteca Central


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Texto completo SciELO Brasil
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Id: lil-595679
Autor: Bouzari, Saeid; Aslani, Mohammad M; Oloomi, Mana; Jafari, Anis; Dashti, Amir.
Título: Comparison of multiplex PCR with serogrouping and PCR-RFLP of fliC gene for the detection of enteropathogenic Escherichia coli (EPEC)
Fonte: Braz. j. infect. dis;15(4):365-369, July-Aug. 2011. ilus, tab.
Idioma: en.
Projeto: Pasteur Institute of Iran.
Resumo: Enteropathogenic Escherichia coli (EPEC) comprise one of the six categories of diarrhoeagenic E. coli (DEC). EPEC is subgrouped into typical (tEPEC) and atypical (aEPEC). The identification of DEC cannot be based only on cultural and biochemical criteria, since they are indistinguishable from the non-pathogenic E. coli commonly found in human feces. Several PCR methods, with both single and multiple target genes, have been reported for detecting the different DEC pathotypes. In the present study five hundred E. coli isolates from children with diarrhea were subjected into multiplex PCR. Furthermore the strains were typed serologically with O antisera and their fliC gene was characterized by PCR-RFLP. The results obtained revealed that overall 41 (8.2 percent) isolates could be detected as EPEC by this multiplex PCR assay. Of these isolates; 27 (66 percent) were typical (escv+, bfp+) and 14 (34 percent) atypical EPEC (escv+, bfp-). None of these 41 isolates contained the Stx1 and Stx2 genes. Among 37 (90 percent) typeable strains, nine different serogroups were present. The most common serogroups were O111, followed by O86, O55 and O119 and 10 different H types were found among these isolates. The multiplex PCR assay was found to be rapid and reliable in comparison to serological test; especially when screening the large number of isolates.
Descritores: DNA Bacteriano/análise
Escherichia coli Enteropatogênica/classificação
Proteínas de Escherichia coli/genética
Reação em Cadeia da Polimerase Multiplex
Antígenos O/análise
Polimorfismo de Fragmento de Restrição
-Diarreia/microbiologia
Escherichia coli Enteropatogênica/genética
Escherichia coli Enteropatogênica/isolamento & purificação
Fezes/microbiologia
Sorotipagem/métodos
Toxina Shiga I/genética
/genética
SHIGA TOXIN TEMEFOS/genética
Limites: Criança
Humanos
Tipo de Publ: Estudo Comparativo
Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


  5 / 6 LILACS  
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Texto completo SciELO Saúde Pública
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Id: lil-331692
Autor: Rodríguez-Angeles, Guadalupe.
Título: Principales características y diagnóstico de los grupos patógenos de Escherichia coli / Principal characteristics and diagnosis of the pathogenic groups of Escherichia coli
Fonte: Salud pública Méx;44(5):464-475, sept.-oct. 2002.
Idioma: es.
Resumo: Escherichia coli colonizes the human intestinal tract within hours of birth and is considered a non-pathogenic member of the normal intestinal flora. However, there are six pathogenic groups that may produce diarrhea: enterotoxigenic (ETEC), enterohemorrhagic (EHEC), enteroinvasive (EIEC), enteropathogenic (EPEC), enteroaggregative (EAEC) and diffusely adherent (DAEC) groups. E. coli can be isolated and classified using traditional methods, by identifying its biochemical or serum characteristics. The pathogenic mechanisms may be studied in cell cultures and animal model assays, as well as more up to date molecular biology methods for study and diagnosis. The latter have proven that genes are involved in pathogenesis. The objective of the present work is to draw attention to the importance of E. coli as a pathogenic organism. This microorganism is an etiologic agent of sporadic cases of diarrhea, hemorrhagic colitis, dysentery, and hemolytic uremic syndromes and outbreaks. Diarrheic E. coli manifestations occur mainly among infants, and deep knowledge and understanding of this microorganism are crucial to better epidemiologic surveillance.
Descritores: Diarreia
Escherichia coli
Infecções por Escherichia coli/microbiologia
-Aderência Bacteriana
Toxinas Bacterianas
Toxinas Bacterianas
Técnicas Bacteriológicas
Diarreia Infantil
Enterotoxinas
Enterotoxinas
Escherichia coli
Escherichia coli
Escherichia coli
Escherichia coli
Escherichia coli
Fermentação
Hemorragia Gastrointestinal
Intestinos
Antígenos O/análise
Sorotipagem
Limites: Adulto
Pré-Escolar
Humanos
Lactente
Responsável: MX1.1 - CENIDSP - Centro de Información para Decisiones en Salud Pública


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Vidotto, Marilda Carlos
Id: lil-279920
Autor: Carbonell, Gleize Villela; Levy, Carlos Emilio; Vidotto, Marilda Carlos.
Título: Resistencia de diferentes sorogrupos de seratia marcescens ao soro humano normal e sua patogenicidade para camundongos / Serratia marcescens: studies on normal human serum resistance, serogrouping and pathogenicity for mice
Fonte: Rev. microbiol;23(2):72-5, abr.-jun. 1992. tab, graf.
Idioma: pt.
Resumo: Isolados nosocomiais de Serratia marcescens pertencentes a diferentes sorogrupos (01, 03, 04, 05, 010, 013, 016, 019 e 06,14) foram analisados quanto a resistencia à açäo bactericida do soro humano (HS). De 60amostras estudadas, 55 (91,6 por cento) foram HS-resistentes para uma concentraçäo de 50 (por cento) de soro e somente amostras do sorogrupo 06,14 foram HS-sensíveis. Todos os isolados de sangue, olhos e ossos foram resistentes ao HS. Amostras com diferentes sensibilidades ao HS foram testadas para a patogenicidade e todas foram letais para camundongos com a mesma DL50 (3,5 x 10(elevado a sétima potencia)). Esses resultados sugerem que a resistência ao soro näo é o principal determinante na virulência de S. marcescens.
Descritores: Camundongos/imunologia
Antígenos O/imunologia
Limites: Animais
Camundongos
Tipo de Publ: Ensaio Clínico Controlado
Estudo Comparativo
Responsável: BR734.1 - Biblioteca Central Cesar Lattes



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