Base de dados : LILACS
Pesquisa : D09.894 [Categoria DeCS]
Referências encontradas : 2 [refinar]
Mostrando: 1 .. 2   no formato [Detalhado]

página 1 de 1

  1 / 2 LILACS  
              next record last record
para imprimir
Texto completo SciELO Brasil
Texto completo
Id: lil-452518
Autor: Cassera, María B; Merino, Emilio F; Peres, Valnice J; Kimura, Emilia A; Wunderlich, Gerhard; Katzin, Alejandro M.
Título: Effect of fosmidomycin on metabolic and transcript profiles of the methylerythritol phosphate pathway in Plasmodium falciparum
Fonte: Mem. Inst. Oswaldo Cruz;102(3):377-384, June 2007. graf, tab.
Idioma: en.
Projeto: Fapesp; . CNPq; . Pronex; . European Commission IC.
Resumo: In Plasmodium falciparum, the formation of isopentenyl diphosphate and dimethylallyl diphosphate, central intermediates in the biosynthesis of isoprenoids, occurs via the methylerythritol phosphate (MEP) pathway. Fosmidomycin is a specific inhibitor of the second enzyme of the MEP pathway, 1-deoxy-D-xylulose-5-phosphate reductoisomerase. We analyzed the effect of fosmidomycin on the levels of each intermediate and its metabolic requirement for the isoprenoid biosynthesis, such as dolichols and ubiquinones, throughout the intraerythrocytic cycle of P. falciparum. The steady-state RNA levels of the MEP pathway-associated genes were quantified by real-time polymerase chain reaction and correlated with the related metabolite levels. Our results indicate that MEP pathway metabolite peak precede maximum transcript abundance during the intraerythrocytic cycle. Fosmidomycin-treatment resulted in a decrease of the intermediate levels in the MEP pathway as well as in ubiquinone and dolichol biosynthesis. The MEP pathway associated transcripts were modestly altered by the drug, indicating that the parasite is not strongly responsive at the transcriptional level. This is the first study that compares the effect of fosmidomycin on the metabolic and transcript profiles in P. falciparum, which has only the MEP pathway for isoprenoid biosynthesis.
Descritores: Eritritol/análogos & derivados
Fosfomicina/análogos & derivados
Plasmodium falciparum/metabolismo
Fosfatos Açúcares/metabolismo
-Genes de Protozoários
Reação em Cadeia da Polimerase
Plasmodium falciparum/efeitos dos fármacos
Plasmodium falciparum/genética
Plasmodium falciparum/crescimento & desenvolvimento
Limites: Animais
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME

  2 / 2 LILACS  
              first record previous record
para imprimir
Texto completo
Id: lil-417582
Autor: Antônio, R. V; Creczynski-Pasa, T. B.
Título: Genetic analysis of violacein biosynthesis by Chromobacterium violaceum
Fonte: Genet. mol. res. (Online);3(1):85-91, Mar. 2004.
Idioma: en.
Resumo: Chromobacterium violaceum presents a distinctive phenotypic characteristic, the production of a deep violet pigment named violacein. Although the physiological function of this pigment is not well understood, the sequencing of the genome of this bacterium has given some insight into the mechanisms and control of violacein production. It was found that erythrose-4-phosphate (E4P), a precursor to aromatic amino acid biosynthesis, is produced by the non-oxidative portion of the hexose monophosphate pathway, since it lacks 6-phosphogluconate dehydrogenase. All genes leading from E4P plus phosphoenolpyruvate to tryptophan are present in the genome. Nevertheless, these genes are not organized in an operon, as in E. coli, indicating that other mechanisms are involved in expression. The sequencing data also indicated the presence and organization of an operon for violacein biosynthesis. Three of the four gene products of this operon presented similarity with nucleotide-dependent monooxygenases and one with a limiting enzyme polyketide synthase. As previously suggested, genes encoding proteins involved in quorum sensing control by N-hexanoyl-homoserine-lactone, an autoinducer signal molecule, are present in the bacterial genome. These data should help guide strategies to increase violacein biosynthesis, a potentially useful molecule
Descritores: Chromobacterium/genética
Complexos Multienzimáticos/biossíntese
Complexos Multienzimáticos/genética
Fosfatos Açúcares/genética
Fosfatos Açúcares/metabolismo
Hidrolases de Éster Carboxílico/biossíntese
Hidrolases de Éster Carboxílico/genética
Tipo de Publ: Research Support, U.S. Gov't, Non-P.H.S.
Responsável: BR1.1 - BIREME

página 1 de 1

Refinar a pesquisa
  Base de dados : Formulário avançado   

    Pesquisar no campo  

Search engine: iAH v2.6 powered by WWWISIS

BIREME/OPAS/OMS - Centro Latino-Americano e do Caribe de Informação em Ciências da Saúde