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Id: biblio-974323
Autor: Wang, Xiuwen; Zhang, Xiaohua; Yao, Qingshou; Hua, Dongliang; Qin, Jiayang.
Título: Comparative proteomic analyses of Hyphozyma roseonigra ATCC 20624 in response to sclareol
Fonte: Braz. j. microbiol;49(supl.1):160-165, 2018. tab, graf.
Idioma: en.
Projeto: National Natural Science Foundation of China; . Shandong Province Science and Technology Project; . Jinan Youth Science and Technology Star Project.
Resumo: Abstract Sclareol is an important intermediate for ambroxide synthesis industries. Hyphozyma roseonigra ATCC 20624 was the only reported strain capable of degrading sclareol to the main product of sclareol glycol, which is the precursor of ambroxide. To date, knowledge is lacking about the effects of sclareol on cells and the proteins involved in sclareol metabolism. Comparative proteomic analyses were conducted on the strain H. roseonigra ATCC 20624 by using sclareol or glucose as the sole carbon source. A total of 79 up-regulated protein spots with a >2.0-fold difference in abundance on 2-D gels under sclareol stress conditions were collected for further identification. Seventy spots were successfully identified and finally integrated into 30 proteins. The up-regulated proteins under sclareol stress are involved in carbon metabolism; and nitrogen metabolism; and replication, transcription, and translation processes. Eighteen up-regulated spots were identified as aldehyde dehydrogenases, which indicating that aldehyde dehydrogenases might play an important role in sclareol metabolism. Overall, this study may lay the fundamentals for further cell engineering to improve sclareol glycol production.
Descritores: Ascomicetos/metabolismo
Proteínas Fúngicas/metabolismo
Diterpenos/metabolismo
-Ascomicetos/genética
Ascomicetos/química
Proteínas Fúngicas/química
Carbono/metabolismo
Eletroforese em Gel Bidimensional
Regulação Fúngica da Expressão Gênica
Proteômica
Glucose/metabolismo
Responsável: BR1.1 - BIREME


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Id: biblio-974297
Autor: Shakibaie, Mojtaba; Ameri, Atefeh; Ghazanfarian, Roya; Adeli-Sardou, Mahboubeh; Amirpour-Rostami, Sahar; Torkzadeh-Mahani, Masoud; Imani, Mehdi; Forootanfar, Hamid.
Título: Statistical optimization of kojic acid production by a UV-induced mutant strain of Aspergillus terreus
Fonte: Braz. j. microbiol;49(4):865-871, Oct.-Dec. 2018. tab, graf.
Idioma: en.
Projeto: Kerman University of Medical Sciences; . Elite Researcher Grant Committee.
Resumo: ABSTRACT The ability of four Aspergillus strains for biosynthesis of kojic acid was evaluated among which Aspergillus terreus represented the highest level (2.21 g/L) of kojic acid production. Improvement kojic acid production ability of A. terreus by random mutagenesis using different exposure time to ultraviolet light (5-40 min) was then performed to obtain a suitable mutant of kojic acid production (designated as C5-10, 7.63 g/L). Thereafter, design of experiment protocol was employed to find medium components (glucose, yeast extract, KH2PO4 (NH4)2SO4, and pH) influences on kojic acid production by the C5-10 mutant. A 25-1 fractional factorial design augmented to central composite design showed that glucose, yeast extract, and KH2PO4 were the most considerable factors within the tested levels (p < 0.05). The optimum medium composition for the kojic acid production by the C5-10 mutant was found to be glucose, 98.4 g/L; yeast extract, 1.0 g/L; and KH2PO4, 10.3 mM which was theoretically able to produce 120.2 g/L of kojic acid based on the obtained response surface model for medium optimization. Using these medium compositions an experimental maximum Kojic acid production (109.0 ± 10 g/L) was acquired which verified the efficiency of the applied method.
Descritores: Pironas/metabolismo
Aspergillus/efeitos da radiação
Aspergillus/metabolismo
-Aspergillus/crescimento & desenvolvimento
Aspergillus/genética
Raios Ultravioleta
Mutagênese
Meios de Cultura/metabolismo
Fermentação
Glucose/metabolismo
Responsável: BR1.1 - BIREME


  3 / 808 LILACS  
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Id: biblio-839369
Autor: Reis, Vanda Renata; Antonangelo, Ana Teresa Burlamaqui Faraco; Bassi, Ana Paula Guarnieri; Colombi, Débora; Ceccato-Antonini, Sandra Regina.
Título: Bioethanol strains of Saccharomyces cerevisiae characterised by microsatellite and stress resistance
Fonte: Braz. j. microbiol;48(2):268-274, April.-June 2017. tab, graf.
Idioma: en.
Projeto: Fundação de Amparo a Pesquisa do Estado de São Paulo.
Resumo: Abstract Strains of Saccharomyces cerevisiae may display characteristics that are typical of rough-type colonies, made up of cells clustered in pseudohyphal structures and comprised of daughter buds that do not separate from the mother cell post-mitosis. These strains are known to occur frequently in fermentation tanks with significant lower ethanol yield when compared to fermentations carried out by smooth strains of S. cerevisiae that are composed of dispersed cells. In an attempt to delineate genetic and phenotypic differences underlying the two phenotypes, this study analysed 10 microsatellite loci of 22 S. cerevisiae strains as well as stress resistance towards high concentrations of ethanol and glucose, low pH and cell sedimentation rates. The results obtained from the phenotypic tests by Principal-Component Analysis revealed that unlike the smooth colonies, the rough colonies of S. cerevisiae exhibit an enhanced resistance to stressful conditions resulting from the presence of excessive glucose and ethanol and high sedimentation rate. The microsatellite analysis was not successful to distinguish between the colony phenotypes as phenotypic assays. The relevant industrial strain PE-2 was observed in close genetic proximity to rough-colony although it does not display this colony morphology. A unique genetic pattern specific to a particular phenotype remains elusive.
Descritores: Saccharomyces cerevisiae/fisiologia
Saccharomyces cerevisiae/genética
Variação Genética
Repetições de Microssatélites
Etanol/metabolismo
-Fenótipo
Saccharomyces cerevisiae/classificação
Saccharomyces cerevisiae/metabolismo
Estresse Fisiológico
Genótipo
Glucose/metabolismo
Concentração de Íons de Hidrogênio
Responsável: BR1.1 - BIREME


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Id: biblio-1039074
Autor: Shah, Rehmat; Subhan, Fazal; Sultan, Syed Muhammad; Haq, Matiul; Ahmad, Hussain; Khan, Qasim Riaz; Ali, Gowhar; Ullah, Sami; Ullah, Ihsan.
Título: Metabolic dysregulation in early onset psychiatric disorder before and after exposure to antipsychotic drugs
Fonte: Braz. J. Pharm. Sci. (Online);55:e17825, 2019. tab, graf.
Idioma: en.
Resumo: Antipsychotic Drugs (APDs) are being widely prescribed to treat various disorders, including schizophrenia and bipolar disorder; however, abnormal glucose metabolism and weight gain have been reported with Atypical Anti-Psychotic drugs (AAPDs) that can lead to insulin-resistance and type 2 diabetes mellitus. The study was designed to assess various biochemical parameters including insulin and blood sugar before and after exposure to APDs in order to exclude the involvement of psychiatric disorders and certain other factors in metabolic dysregulations. Fifty seven APDs-naïve patients with first episode psychosis were divided into six groups who received olanzapine, quetiapine, risperidone, aripiprazole, haloperidol or combination of olanzapine with escitalopram and haloperidol. The serum samples were taken before the intake of the first dose and then on follow-up. Decrease in the level of elevated insulin and glucose was observed post-treatment in some patients, while others were observed whose insulin and glucose levels increased post-treatment, yet some patients did not show any disturbance in the insulin and glucose levels. It is concluded that psychiatric disorders by itself, narcotics, cigarette smoking and use of oral snuff may be also be implicated in metabolic dysregulations. The effects of APDs on insulin and glucose in healthy volunteers might be different than in patients with psychiatric disorders.
Descritores: Antipsicóticos/análise
Antipsicóticos/efeitos adversos
Glucose/efeitos adversos
Insulina/efeitos adversos
-Pâncreas/efeitos dos fármacos
Análise de Variância
Risperidona/efeitos adversos
Fumarato de Quetiapina/efeitos adversos
/efeitos adversos
FRENCH GUIANATEMEFOS/efeitos adversos
Limites: Seres Humanos
Masculino
Feminino
Adolescente
Responsável: BR40.1 - DBD - Divisão de Biblioteca e Documentacão do Conjunto das Químicas


  5 / 808 LILACS  
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Id: lil-530678
Autor: Vicente, Priscila Cadima; Caramori, Jacqueline Costa Teixeira; Franco, Sonia Regina Verde da Silva; Vicente, Eduardo José Danza; Pereira, José Jonas; Santos, Sueli Maria dos Reis.
Título: Efeito da glicose na histomorfologia do peritônio durante a diálise peritoneal / Glucose effects on the histomorfology of the peritoneum during peritonealdialysis
Fonte: HU rev;34(1):27-31, jan.-mar. 2008. ilus.
Idioma: pt.
Resumo: As alterações peritoneais em pacientes submetidos à diálise peritoneal por longos períodos começam com as modificações mesoteliais e, mais tarde, envolvem alterações submesoteliais, fibrose e vasculopatias. Entre outros fatores, essas alterações podem ser decorrentes da alta concentração de glicose da solução de diálise. Desta forma, o objetivo do presente estudo foi estudar as alterações histomorfológicas na membrana peritoneal de ratos submetidos à diálise peritoneal, com solução de glicose a temperatura ambiente. Foram utilizados 20 ratos Wistar divididos em dois grupos: grupo controle (GC) com oito animais que não receberam nenhuma injeção intraperitoneal, e grupo solução concentrada (GSC) com 12 animais que receberam 10ml de fluido de diálise com 4,25% de glicose uma vez por dia durante 30 dias. Nos animais do GSC, o peritônio visceral do baço e o parietal da parede abdominal apresentaram alterações epiteliais com transformação cúbica das células mesoteliais e espessamento na camada submesotelial, respectivamente. Esses resultados sugerem que a solução de diálise com 4,25% de glicose em temperatura ambiente realizada uma vez por dia durante 30 dias pode alterar a histomorfologia do peritônio.

Alterations in the peritoneum of patients submitted to peritoneal dialysis during long periods begins with modifications of the mesothelium, and latter unfolds to alterations of the submesothelium, of the vascular system and fibrosis. One of the possible explications to these alterations is the high concentration of glucose in the dialysis solution. Therefore, the aim of this study was to study the histomorphological alterations of the peritoneal membrane in rats submitted to peritoneal dialysis, with the glucose solution at the local temperature. 20 Wistar rats were used, divided into two groups: control group (CG) with eight animals that didn't receive intraperitoneal injection and the concentrated solution group (CSG) with12 animals that received 10ml of the dialysis fluid with 4.25% of glucose, once a day, for 30 days. The visceral peritoneum of the spleen and the parietal peritoneum of the abdominal wall of the animals of CSG showed alterations of the epithelial layer with cubic transformation of the mesothelium cells and the thickness of the submesothelium layer, respectively. The dialysis solution of glucose at the concentration of 4.25%, at local temperature, applied once a day, during 30 days, altered the histomorphology of the peritoneum.
Descritores: Glucose/efeitos adversos
-Peritônio
Diálise Peritoneal
Limites: Seres Humanos
Tipo de Publ: Relatos de Casos
Responsável: BR378.1 - Biblioteca Central


  6 / 808 LILACS  
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Id: lil-233914
Autor: Barboza, Ruy.
Título: Modelo linear-por-partes da membrana da célula pancreática ß / Piecewise linear model of pancreatic ß cells membrane
Fonte: In: Schiabel, Homero; Slaets, Annie France Frère; Costa, Luciano da Fontoura; Baffa Filho, Oswaldo; Marques, Paulo Mazzoncini de Azevedo. Anais do III Fórum Nacional de Ciência e Tecnologia em Saúde. Säo Carlos, s.n, 1996. p.652-652, ilus, graf.
Idioma: pt.
Conferência: Apresentado em: Fórum Nacional de Ciência e Tecnologia em Saúde, 3 e Congresso Brasileiro de Engenharia Biomédica, 15 e Congresso Brasileiro de Físicos em Medicina , 6 e Congresso Brasileiro de Informática em Saúde, 5 e Encontro Brasileiro de Proteçäo Radiológica, Campos do Jordäo, 13-17 out. 1996.
Resumo: Um sistema de três equações diferenciais contendo apenas relações lineares e funções lineares-por-partes é apresentado como modelo para descrever o comportamento oscilatório das células Beta do pâncreas. As equações foram obtidas através de simplificação sistemática do modelo mais rigoroso de Chay.
Descritores: Modelos Lineares
Ilhotas Pancreáticas
-Cálcio
Glucose
Axônios
Potássio
Responsável: BR1.1 - BIREME
BR1.1/3012.106


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Id: lil-730626
Autor: Cardoso, Maria Vera Lúcia Moreira Leitão; Farias, Leiliane Martins; Melo, Gleicia Martins de.
Título: Music and 25% glucose pain relief for the premature infant: a randomized clinical trial / Música e glicose 25% no alívio da dor dos prematuros: ensaio clínico randomizado / Música y glucosa al 25% en el alivio del dolor de los prematuros: ensayo clínico aleatorio
Fonte: Rev. latinoam. enferm. (Online);22(5):810-818, Sep-Oct.2014. tab.
Idioma: en.
Projeto: CNPq.
Resumo: OBJECTIVE: to analyze the total Premature Infant Pain Profile scores of premature infants undergoing arterial puncture during music and 25% glucose interventions, and to assess their association with neonatal and therapeutic variables. METHOD: a randomized clinical trial with 80 premature infants; 24 in the Experimental Group 1 (music), 33 in the Experimental Group 2 (music and 25% glucose), 23 in the Positive Control Group (25% glucose). All premature infants were videotaped and a lullaby was played for ten minutes before puncture in Experimental Groups 1 and 2; 25% glucose administered in Experimental Group 2 and the Positive Control Group two minutes before puncture. RESULTS: 60.0% of premature infants had moderate or maximum pain; pain scores and intervention groups were not statistically significant. Statistically significant variables: Experimental Group 1: head and chest circumference, Apgar scores, corrected gestational age; Experimental Group 2: chest circumference, Apgar scores, oxygen therapy; Positive Control group: birth weight, head circumference. CONCLUSION: neonatal variables are associated with pain in premature infants. Brazilian Registry of Clinical Trials: UTN: U1111-1123-4821. .

OBJETIVO: analisar os escores totais do Premature Infant Pain Profile dos prematuros submetidos a punção arterial sob intervenção da música e glicose 25%, verificar a associação desses com variáveis neonatais e terapêuticas. MÉTODO: ensaio clínico randomizado com 80 prematuros, 24 Grupo Experimental 1 (música), 33 Grupo Experimental 2 (música e glicose 25%), 23 Grupo Controle Positivo (glicose 25%). Todos os prematuros foram filmados e uma música de ninar foi ouvida por 10 minutos antes da punção no Grupo Experimental 1 e Grupo Experimental 2, glicose 25% administrada no Grupo Experimental 2 e Grupo Controle Positivo dois minutos antes da punção. RESULTADOS: 60,0% dos prematuros apresentaram dor moderada ou intensa, os escores de dor e grupos de intervenção não demonstraram significância estatística. Variáveis estatisticamente significantes, Grupo Experimental 1: perímetro cefálico e torácico, Apgar, idade gestacional corrigida; Grupo Experimental 2: perímetro torácico, Apgar, oxigenoterapia; Grupo Controle Positivo: peso nascimento, perímetro cefálico. CONCLUSÃO: variáveis neonatais apresentam associação com dor em prematuros. Registro Brasileiro de Ensaio Clínico: UTN: U1111-1123-4821. .

OBJETIVO: analizar los puntajes totales del Premature Infant Pain Profile de los prematuros sometidos a punción arterial bajo intervención de música y de glucosa al 25%, y verificar la asociación de estos con variables neonatales y terapéuticas. MÉTODO: ensayo clínico aleatorio con 80 prematuros: 24 Grupo Experimental 1 (música), 33 Grupo Experimental 2 (música y glucosa al 25%) y 23 Grupo Control Positivo (glucosa al 25%). Todos los prematuros fueron filmados y una canción de cuna fue escuchada por 10 minutos antes de la punción en el Grupo Experimental 1 y en el Grupo Experimental 2, glucosa al 25% administrada en el Grupo Experimental 2 y en el Grupo Control Positivo, dos minutos antes de la punción. RESULTADOS: 60,0% de los prematuros presentaron dolor moderado o intenso, los puntajes de dolor y los grupos de intervención no demostraron ser estadísticamente significativos. Las variables estadísticamente significativas fueron en el Grupo Experimental 1: perímetro cefálico y torácico, Apgar, Edad Gestacional corregida; en el Grupo Experimental 2: perímetro torácico, Apgar, oxigenoterapia; y, en el Grupo Control Positivo: peso nacimiento, perímetro cefálico. CONCLUSIÓN: las variables neonatales presentan una asociación con el dolor en prematuros. Registro Brasileño de Ensayo Clínico: UTN: U1111-1123-4821. .
Descritores: Manejo da Dor/métodos
Glucose/administração & dosagem
Musicoterapia
-Recém-Nascido Prematuro
Método Duplo-Cego
Limites: Seres Humanos
Masculino
Feminino
Recém-Nascido
Tipo de Publ: Research Support, Non-U.S. Gov't
Ensaio Clínico Controlado Aleatório
Responsável: BR1.1 - BIREME


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Id: biblio-1022118
Autor: Silva Lemões, Juliana; Lemons e Silva, Claudia Fernanda; Farias Avila, Sabrina Peres; Scherrer Montero, Cândida Raquel; Anjos e Silva, Sérgio Delmar dos; Samios, Dimitrios; Ruaro Peralba, Maria do Carmo.
Título: Chemical pretreatment of Arundo donax L. for second-generation ethanol production
Fonte: Electron. j. biotechnol;31:67-54, Jan. 2018. ilus, tab, graf.
Idioma: en.
Projeto: Conselho Nacional de Desenvolvimento Científico e Tecnológico.
Resumo: Background: Pretreatment of lignocellulosic biomass is essential for using it as a raw material for chemical and biofuel production. This study evaluates the effects of variables in the chemical pretreatment of the Arundo biomass on the glucose and xylose concentrations in the final enzymatic hydrolysate. Three pretreatments were tested: acid pretreatment, acid pretreatment followed by alkaline pretreatment, and alkaline pretreatment. Results: The amounts of glucose and xylose released by the enzymatic hydrolysis of the Arundo biomass obtained from acid pretreatment ranged from 6.2 to 19.1 g/L and 1.8 to 3.1 g/L, respectively. The addition of alkaline pretreatment led to a higher yield from the enzymatic hydrolysis, with the average glucose concentration 3.5 times that obtained after biomass hydrolysis with an acid pretreatment exclusively. The use of an alkaline pretreatment alone resulted in glucose and xylose concentrations similar to those obtained in the two-step pretreatment: acid pretreatment followed by alkaline pretreatment. There was no significant difference in 5-hydroxymethylfurfural, furfural, or acetic acid concentrations among the pretreatments. Conclusion: Alkaline pretreatment was essential for obtaining high concentrations of glucose and xylose. The application of an alkaline pretreatment alone resulted in high glucose and xylose concentrations. This result is very significant as it allows a cost reduction by eliminating one step.
Descritores: Etanol/metabolismo
Poaceae/química
-Ácidos/química
Xilose/análise
Celulose/química
Biomassa
Biocombustíveis
Glucose/análise
Hidrólise
Lignina
Responsável: CL1.1 - Biblioteca Central


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Id: biblio-1022044
Autor: Revin, Victor; Atykyan, Nelli; Lyovina, Ekaterina; Dragunova, Yuliya; Ushkina, Victoriya.
Título: Effect of ultraviolet radiation on physiological and biochemical properties of yeast Saccharomyces cerevisiae during fermentation of ultradispersed starch raw material
Fonte: Electron. j. biotechnol;31:61-66, Jan. 2018. graf, ilus, tab.
Idioma: en.
Projeto: Ministry of Education and Science of the Russian Federation.
Resumo: Background: Study of correlation between pretreatment of yeast with ultraviolet radiation and efficiency of further fermentation of wort made of ultrafine grain particles to ethanol. Results: We investigated three races of industrial yeast Saccharomyces cerevisiae (native and irradiated by ultraviolet). Physiological properties during fermentation of starchy wort were tested in all variants. It was shown that activation of the yeast by ultraviolet radiation allows to further increase the ethanol yield by 25% on average compared with the native yeast races when using thin (up to micro- and nano-sized particles) or standard grain grinding. Conclusions: Using mechanical two-stage grinding of starchy raw materials and ultraviolet pretreatment of yeast, the efficiency of saccharification of starch and fermentation of wort to ethanol was increased.
Descritores: Saccharomyces cerevisiae/efeitos da radiação
Raios Ultravioleta
Leveduras/efeitos da radiação
Etanol/efeitos da radiação
-Saccharomyces/metabolismo
Amido
Temperatura Ambiente
Leveduras/metabolismo
Estabilidade Enzimática
Etanol/metabolismo
Fermentação
Glucose
Amilases
Responsável: CL1.1 - Biblioteca Central


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Id: biblio-1021461
Autor: Soo, Chiu-Shyan; Yap, Wai-Sum; Hon, Wei-Min; Ramli, Norhayati; Md Shah, Umi Kalsom; Phang, Lai-Yee.
Título: Co-production of hydrogen and ethanol by Escherichia coli SS1 and its recombinant
Fonte: Electron. j. biotechnol;30:64-70, nov. 2017. ilus, graf, tab.
Idioma: en.
Projeto: Fundamental Research Grant Scheme.
Resumo: Background: The development of a potential single culture that can co-produce hydrogen and ethanol is beneficial for industrial application. Strain improvement via molecular approach was proposed on hydrogen and ethanol co-producing bacterium, Escherichia coli SS1. Thus, the effect of additional copy of native hydrogenase gene hybC on hydrogen and ethanol co-production by E. coli SS1 was investigated. Results: Both E. coli SS1 and the recombinant hybC were subjected to fermentation using 10 g/L of glycerol at initial pH 7.5. Recombinant hybC had about 2-fold higher cell growth, 5.2-fold higher glycerol consumption rate and 3-fold higher ethanol productivity in comparison to wild-type SS1. Nevertheless, wild-type SS1 reported hydrogen yield of 0.57 mol/mol glycerol and ethanol yield of 0.88 mol/mol glycerol, which were 4- and 1.4-fold higher in comparison to recombinant hybC. Glucose fermentation was also conducted for comparison study. The performance of wild-type SS1 and recombinant hybC showed relatively similar results during glucose fermentation. Additional copy of hybC gene could manipulate the glycerol metabolic pathway of E. coli SS1 under slightly alkaline condition. Conclusions: HybC could improve glycerol consumption rate and ethanol productivity of E. coli despite lower hydrogen and ethanol yields. Higher glycerol consumption rate of recombinant hybC could be an advantage for bioconversion of glycerol into biofuels. This study could serve as a useful guidance for dissecting the role of hydrogenase in glycerol metabolism and future development of effective strain for biofuels production.
Descritores: Etanol/metabolismo
Escherichia coli/metabolismo
Hidrogênio/metabolismo
Hidrogenase/metabolismo
-Recombinação Genética
Biodegradação Ambiental
Meios de Cultura
Escherichia coli/enzimologia
Alcalinização
Fermentação
Glucose/metabolismo
Glicerol/metabolismo
Hidrogenase/genética
Responsável: CL1.1 - Biblioteca Central



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