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Id: biblio-1253015
Autor: Usta-Gorgun, Buse; Yilmaz-Ersan, Lutfiye.
Título: Short-chain fatty acids production by Bifidobacterium species in the presence of salep
Fonte: Electron. j. biotechnol;47:29-35, sept. 2020. tab, graf.
Idioma: en.
Projeto: Commission of Scientific Research Projects of Bursa Uludag University, Bursa, Turkey.
Resumo: BACKGROUND: Salep is obtained by grinding dried orchid tubers and used as a valuable ingredient in the food industry. Because of the glucomannan content of salep, it is thought to have prebiotic potential. However, there is little information in studies concerning the fermentation characteristics and potential prebiotic properties of salep. The objective of this study was to investigate the effect of salep on bifidobacterial growth by measuring the highest optical density (OD), calculating the specific growth rates, and determining the production of lactic acid and short-chain fatty acids (acetic, propionic, and butyric acid) as a result of bacterial fermentation. RESULT: The OD and pH values obtained in this study showed that salep was utilized as a source of assimilable carbon and energy by the Bifidobacterium species (BS). All Bifidobacterium strains produced lactic, acetic, propionic, and butyric acid, indicating that salep is readily fermented by these bacteria. Salep at 1% (w/v) showed a similar effect on bifidobacterial growth as that promoted by 1% (w/v) glucose used as a traditional carbon source. CONCLUSIONS: Bifidobacterium species can develop in media containing salep as well as in glucose and exhibit the potential to be used as new sources of prebiotics.
Descritores: Pós/metabolismo
Bifidobacterium/crescimento & desenvolvimento
Bifidobacterium/metabolismo
Ácidos Graxos Voláteis/biossíntese
-Propionatos/análise
Propionatos/metabolismo
Indústria Alimentícia
Ácido Acético/análise
Ácido Acético/metabolismo
Ácido Láctico/análise
Ácido Láctico/metabolismo
Probióticos
Ácido Butírico/análise
Ácido Butírico/metabolismo
Ácidos Graxos Voláteis/análise
Prebióticos
Fermentação
Concentração de Íons de Hidrogênio
Responsável: CL1.1 - Biblioteca Central


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Id: biblio-1052032
Autor: Reyes-Contreras, Carolina; Leiva, Ana María; Vidal, Gladys.
Título: Evaluation of triclosan toxic effects on the methanogenic activity
Fonte: Electron. j. biotechnol;39:61-66, may. 2019. graf, tab.
Idioma: en.
Projeto: CONICYT (Chile).
Resumo: BACKGROUND: Triclosan (TCS) is an antimicrobial agent widely used in health care and consumer products. This compound is present in sludge of wastewater treatment plants (WWTPs), and because of its bactericidal characteristics, it can inhibit the methanogenic activity in anaerobic digestion (AD) technology. The aim of this study was to evaluate the toxic effects of TCS on the methanogenic activity. RESULTS: Batch anaerobic reactors were used with TCS concentrations of 7.8, 15.7, 23.5, and 31.4 mg/L. These assays consisted in three successive feedings (I, II, and III), wherein the sludge was exposed to each TCS concentration and volatile fatty acid (VFA) substrate. For evaluation of the residual sludge activity during feeding III, only VFA was used. The results showed that the increase in TCS concentrations correlated with the reduction in methane (CH4) production. In this case, the minimum values were achieved for TCS concentration of 31.4 mg/L with CH4 levels between 101.9 and 245.3 during feedings I, II, and III. Regarding the effect of TCS on VFA consumption, an inhibitory effect was detected for TCS concentrations of 23.5 and 31.4 mg/L, with concentrations of acetic, butyric, and propionic acids at the end of the assay (37 d) between 153.6 and 206.8, 62.5 and 60.1, and 93.4 and 110 mg/L, respectively. Regarding the removal of TCS during AD, these values were above 47%. Conclusion: TCS is an inhibitor of methanogenic activity with a decrease between 63 and 70% during the different feedings. The CH4 production was not recovered during feeding III, with inhibition percentages of 21­72%.
Descritores: Triclosan/toxicidade
Digestão Anaeróbia
Metano/metabolismo
Anti-Infecciosos/toxicidade
-Esgotos
Estações de Tratamento de Águas Residuárias
Cromatografia Líquida de Alta Pressão
Ácidos Graxos Voláteis
Anaerobiose
Responsável: CL1.1 - Biblioteca Central


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Texto completo
Id: biblio-974326
Autor: Fraga, Martín; Fernández, Sofía; Perelmuter, Karen; Pomiés, Nicolle; Cajarville, Cecilia; Zunino, Pablo.
Título: The use of Prevotella bryantii 3C5 for modulation of the ruminal environment in an ovine model
Fonte: Braz. j. microbiol;49(supl.1):101-106, 2018. tab, graf.
Idioma: en.
Projeto: Agencia Nacional de Investigación.
Resumo: Abstract In the Southern Hemisphere, ruminants are mostly raised in grazing systems where animals consume forage and are supplemented with low amounts of concentrates. Concentrates are usually given separately and are rapidly ingested. This practice leads to changing rumen environment conditions during the day, may alter the rumen microbial metabolism and could affect host performance. The native ruminal Prevotella bryantii strain 3C5 was administered every 48 h to wethers under experimental conditions simulating Southern-Hemisphere feeding to evaluate its potential as a rumen fermentation modulator. The inoculum potential was assessed on day 17. The ammonia nitrogen (NH3-N), volatile fatty acids and ruminal pH were monitored on a 24-h basis 19 days after the beginning of the experiment, and the microbial community structure was assessed by pyrosequencing. The administration of P. bryantii modified the fermentation products and daily pH values compared to the control. The NH3-N concentration in the rumen of treated animals was significantly higher than that of the untreated animals. Modification of the ruminal environment and fermentation pathways was achieved without altering the general structure of the microbial community or the potential methane production. P. bryantii 3C5 could be considered in potential probiotic formulations for ruminants in semi-intensive systems.
Descritores: Rúmen/microbiologia
Prevotella/metabolismo
-Rúmen/metabolismo
Rúmen/química
Ovinos
Prevotella/genética
Digestão
Ácidos Graxos Voláteis/metabolismo
Fermentação
Amônia/metabolismo
Concentração de Íons de Hidrogênio
Modelos Biológicos
Limites: Animais
Masculino
Responsável: BR1.1 - BIREME


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Id: biblio-974307
Autor: Zhang, Nan; Peng, Huijuan; Li, Yong; Yang, Wenxiu; Zou, Yuneng; Duan, Huiguo.
Título: Ammonia determines transcriptional profile of microorganisms in anaerobic digestion
Fonte: Braz. j. microbiol;49(4):770-776, Oct.-Dec. 2018. tab, graf.
Idioma: en.
Projeto: Key Project of Education Department of Sichuan Province; . Major Cultivation Project of Education Department of Sichuan Province.
Resumo: ABSTRACT Anaerobic digestion is important for the management of livestock manure with high ammonia level. Although ammonia effects on anaerobic digestion have been comprehensively studied, the molecular mechanism underlying ammonia inhibition still remains elusive. In this study, based on metatranscriptomic analysis, the transcriptional profile of microbial community in anaerobic digestion under low (1500 mg L-1) and high NH4 + (5000 mg L-1) concentrations, respectively, were revealed. The results showed that high NH4 + concentrations significantly inhibited methane production but facilitated the accumulations of volatile fatty acids. The expression of methanogenic pathway was significantly inhibited by high NH4 + concentration but most of the other pathways were not significantly affected. Furthermore, the expressions of methanogenic genes which encode acetyl-CoA decarbonylase and methyl-coenzyme M reductase were significantly inhibited by high NH4 + concentration. The inhibition of the co-expressions of the genes which encode acetyl-CoA decarbonylase was observed. Some genes involved in the pathways of aminoacyl-tRNA biosynthesis and ribosome were highly expressed under high NH4 + concentration. Consequently, the ammonia inhibition on anaerobic digestion mainly focused on methanogenic process by suppressing the expressions of genes which encode acetyl-CoA decarbonylase and methyl-coenzyme M reductase. This study improved the accuracy and depth of understanding ammonia inhibition on anaerobic digestion.
Descritores: Bactérias/genética
Bactérias/metabolismo
Amônia/metabolismo
-Bactérias/isolamento & purificação
Bactérias/classificação
Transcrição Genética
Reatores Biológicos/microbiologia
Ácidos Graxos Voláteis/metabolismo
Microbiota
Anaerobiose
Metano/metabolismo
Responsável: BR1.1 - BIREME


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Caliari, M. V
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Id: lil-483251
Autor: Costa, S. F; Pereira, M. N; Melo, L. Q; Caliari, M. V; Chaves, M. L.
Título: Alterações morfológicas induzidas por butirato, propionato e lactato sobre a mucosa ruminal e epiderme de bezerros: II. Aspectos ultra-estruturais / Lactate, propionate, and butyrate induced morphological alterations on calf ruminal mucosa and epidermis: II. Ultra-structurals aspects
Fonte: Arq. bras. med. vet. zootec;60(1):10-18, fev. 2008. ilus, tab.
Idioma: pt.
Projeto: FAPEMIG; . CNPq.
Resumo: Avaliou-se o efeito de ácidos graxos voláteis (AGV) sobre a integridade do epitélio no rúmen, no plano nasolabial, na epicera e no perioplum traseiro e dianteiro de bezerros e validou-se a feitura de biópsias tegumentares como indicadores de alterações morfológicas da mucosa ruminal. Dezessete bezerros, com sonda no rúmen, receberam infusões intra-ruminais de AGV ou salina, durante 37 dias. Aos 89 dias de vida, após o abate, foram colhidas amostras dos tecidos. Os AGV aumentaram a área de epitélio total e a área de células metabolicamente ativas no epitélio ruminal, embora o butirato não tenha induzido ao desenvolvimento papilar. A área de epitélio não queratinizado no plano nasolabial foi reduzida pela infusão de AGV. Butirato e lactato foram mais indutores de alterações patológicas no epitélio ruminal. Não foram observadas lesões histológicas nos epitélios do plano nasolabial, da epicera e do perioplum, mostrando que essas são conseqüências do efeito direto dos AGV sobre o epitélio ruminal. Os efeitos indireto e direto dos AGV sobre a morfologia dos tecidos epiteliais queratinizados não foram iguais. Biópsias tegumentares podem ter utilidade como indicadores de alterações morfológicas da mucosa ruminal.

The effect of volatile fatty acids (VFA) on rumen wall, epidermis of nasolabial surface, perioplum, and epicera of calves was evaluated. The experiment also aimed to validate the procedure of tegument biopsies as indicators of ruminal mucosa alterations. Seventeen neonatal calves with foley catheters received intraruminal infusions of VFA or saline, during 37 days. At 89-day-old, the animals were slaughtered and tissue samples were collected from rumen, nasolabial surface, epicera, and perioplum from face and hindquarters. VFA infusion increased total epithelium area and metabolically active ruminal cell area; although butirate did not induce the papilar development. The effect of nasolabial surface VFA infusion was the opposite to that observed in the rumen. No histological lesion was observed on nasolabial surface, epicera, and perioplum, demonstrating that these are consequences of VFA direct effect on ruminal epithelium. Butyrate and lactate induced more alterations on the ruminal epithelium. Indirect and direct VFA effects on keratinized epithelium tissues morphology were not identical. Tegumentary biopsies may be useful as indicators of morphological alterations of ruminal mucosa.
Descritores: Anatomia Veterinária
Bovinos
Pele
Rúmen/anatomia & histologia
Rúmen/ultraestrutura
Ácidos Graxos Voláteis/efeitos adversos
Limites: Animais
Responsável: BR68.1 - Biblioteca Virginie Buff D'Ápice


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Id: biblio-1015999
Autor: Ahmadi, Negin; Khosravi-Darani, Kianoush; Mortazavian, Amir Mohammad.
Título: An overview of biotechnological production of propionic acid: from upstream to downstream processes
Fonte: Electron. j. biotechnol;28:67-75, July. 2017.
Idioma: en.
Resumo: The increasing demand for propionic acid (PA) production and its wide applications in several industries, especially the food industry (as a preservative and satiety inducer), have led to studies on the low-cost biosynthesis of this acid. This paper gives an overview of the biotechnological aspects of PA production and introduces Propionibacterium as the most popular organism for PA production. Moreover, all process variables influencing the production yield, different simple and complex carbon sources, the metabolic pathway of production, engineered mutants with increased productivity, and modified tolerance against high concentrations of acid have been described. Furthermore, possible methods of extraction and analysis of this organic acid, several applied bioreactors, and different culture systems and substrates are introduced. It can be concluded that maximum biomass and PA production may be achieved using metabolically engineered microorganisms and analyzing the most significant factors influencing yield. To date, the maximum reported yield for PA production is 0.973 g·g-1, obtained from Propionibacterium acidipropionici in a three-electrode amperometric culture system in medium containing 0.4 mM cobalt sepulchrate. In addition, the best promising substrate for PA bioproduction may be achieved using glycerol as a carbon source in an extractive continuous fermentation. Simultaneous production of PA and vitamin B12 is suggested, and finally, the limitations of and strategies for competitive microbial production with respect to chemical process from an economical point of view are proposed and presented. Finally, some future trends for bioproduction of PA are suggested.
Descritores: Propionatos/metabolismo
Propionibacterium/metabolismo
-Propionatos/química
Vitamina B 12/biossíntese
Carbono/metabolismo
Reatores Biológicos
Ácidos Graxos Voláteis/metabolismo
Fermentação
Concentração de Íons de Hidrogênio
Nitrogênio/metabolismo
Tipo de Publ: Revisão
Responsável: CL1.1 - Biblioteca Central


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Texto completo SciELO Chile
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Id: lil-451624
Autor: Valenzuela B, Andrea; Maiz G, Alberto.
Título: El rol de la fibra dietética en la nutrición enteral / Role of dietary fiber in enteral nutrition
Fonte: Rev. chil. nutr;33(supl.2):342-311, nov. 2006. tab, graf.
Idioma: es.
Resumo: La fibra dietética está constituida por un grupo heterogéneo de sustancias de origen vegetal que son resistentes a la digestión y absorción en el intestino delgado, pero que sufren una digestión parcial o total en el colon. La fibra insoluble ayuda a mantener un tránsito intestinal normal y la soluble tiene efectos beneficiosos sobre la microflora del colon donde es fermentada generando ácidos grasos de cadena corta (AGCC). Los AGCC son substratos preferenciales para las células intestinales. A pesar del conocimiento sobre los efectos beneficiosos de la fibra, las fórmulas de nutrición enteral (NE) que rutinariamente se indican, no la contienen. Recientemente, se ha incorporado fibra soluble (fructo-oligosacáridos -FOS-) en algunas de estas fórmulas, siendo ellas de utilidad para el manejo de diarreas en pacientes que reciben NE. Además, estas fórmulas parecen ser útiles en el tratamiento de pacientes con enfermedades intestinales inflamatorias y en el síndrome de intestino corto.
Descritores: Oligossacarídeos
Fibras na Dieta
Nutrição Enteral
Impactos na Saúde
Ácidos Graxos Voláteis
Limites: Humanos
Tipo de Publ: Artigo Clássico
Responsável: CL334.1 - Biblioteca UBO


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Id: biblio-837110
Autor: Melo, Illana Louise Pereira de.
Título: Avaliação em ratos do efeito do óleo da semente de romã ( Punica granatum L. ) sobre o perfil lipídico tecidual e sua influência sobre parâmetros bioquímicos em processos oxidativos / Evaluation of the effects of pomegranate seed oil (Punica granatum L. ) on tissue lipid profile and its influence on biochemical parameters in oxidative processes of rats.
Fonte: São Paulo; s.n; nov. 30, 2012. 169 p. tab, graf, ilus.
Idioma: pt.
Tese: Apresentada a Universidade de São Paulo. Faculdade de Ciências Farmacêuticas para obtenção do grau de Doutor.
Resumo: O objetivo geral deste estudo foi avaliar, em ratos, o efeito do óleo da semente de romã (PSO) sobre o perfil lipídico tecidual e sua influência sobre parâmetros bioquímicos em processos oxidativos. Foi realizada a caracterização do PSO, confirmando a presença do ácido punícico (PA; 55%) como ácido graxo majoritário e a alta concentração de fitosteróis (539mg/100g), bem como a presença de vitamina E (175mg/100g). O PSO apresentou-se dentro dos padrões de qualidade e a sua estabilidade oxidativa foi melhor em comparação ao óleo de linhaça. A suplementação de ratos saudáveis com o PSO, por via intragástrica durante 40 dias, não afetou o ganho de peso total e o peso dos tecidos muscular (gastrocnêmio) e adiposos (epididimal e retroperitonial). No entanto o PA foi metabolizado e incorporado na forma de ácido linoléico conjugado, sendo dose-dependete nos tecidos hepático, muscular, cardíaco, renal e adiposos. No cérebro, não foram observados ácidos graxos conjugados, mas as substâncias reativas ao ácido tiobarbitúrico (TBARS) apresentaram-se significativamente reduzidas nos animais suplementados com PSO, em relação ao controle. De modo geral, os resultados mostram que o PSO não provoca alterações no metabolismo lipídico e não participa do processo de inibição da oxidação em animais saudáveis. Em ratos submetidos ao estresse oxidativo hepático pelo tetracloreto de carbono (CCl4), a suplementação com PSO durante 21 dias não foi capaz de prevenir o quadro de estresse oxidativo, indicando que este óleo não tem efeito antioxidante utilizando esse modelo animal; embora a análise histológica tenha mostrado menores áreas lesionadas no parênquima hepático nos grupos tratados. Os resultados obtidos neste trabalho contribui com a literatura fornecendo mais informações a respeito do uso dos ácidos graxos conjugados, bem como do PSO em organismos saudáveis e submetidos à estresse oxidativo

The aim of this study was to evaluate the effect of pomegranate seed oil (PSO) on tissue lipid profile and its influence on biochemical parameters in oxidative processes of Wistar rats. Characterization of PSO was carried out, confirming the presence of the punicic acid (PA, 55%) as the major fatty acid present in the oil and high concentrations of phytosterols (539mg/100g) were also observed, as well as the presence of vitamin E (175mg/100g). The PSO was within quality standards and it presented a higher oxidative stability as compared to flaxseed oil. The supplementation of healthy rats with the PSO via gavage during 40 days did not affect weight gain and total weight of muscle (gastrocnemius) and adipose (epididymal and retroperitoneal) tissues. However, PA was metabolized and incorporated as CLAs in a dose-dependent manner in the liver, muscle, heart, kidney and adipocytes. In the brain, conjugated fatty acids were not detected, but the values of thiobarbituric acid reactive substances were significantly reduced in animals supplemented with PSO as compared to the control group. Overall, the results showed that the PSO caused no changes in the lipid metabolism and did not inhibit tne oxidation in healthy animals. In rats that underwent hepatic oxidative stress by carbon tetrachloride (CCl4), the PSO supplementation for 21 days was not able to prevent the oxidative stress, indicating that this oil has no antioxidant effect using this animal model; although histological analysis has shown less injured areas in the liver parenchyma in the test groups. The results obtained in this study are a good addition to the literature once it provided more information about the use of conjugated fatty acids as well as garnered useful information about the effects of consumption of PSO in oxidative stress-induced rats
Descritores: Ácido Graxo Sintases
Óleos Voláteis/química
Estresse Oxidativo
Lythraceae/química
-Fenômenos Bioquímicos
Ácidos Graxos Voláteis/análise
Óleos Voláteis/análise
Sementes/química
Limites: Animais
Ratos
Tipo de Publ: Estudo de Avaliação
Responsável: BR40.1 - DBD - Divisão de Biblioteca e Documentacão do Conjunto das Químicas


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Texto completo SciELO Brasil
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Id: biblio-833807
Autor: Jovaisiene, J; Bakutis, B; Baliukoniene, V; Matusevicius, P; Lipinski, K; Antoszkiewicz, Z; Fijalkowska, M.
Título: Biogenic amines and mycotoxins concentrations in baled silage from organic and conventional farms / Concentrações de aminas biogênicas e micotoxinas em silagem embalada de fazendas orgânicas e convencionais
Fonte: Arq. bras. med. vet. zootec;69(2):269-277, mar.-abr. 2017. tab.
Idioma: en.
Resumo: The aim of the current study was to investigate biogenic amines and mycotoxins concentrations in baled silage (mainly Poaceae family grasses) prepared in organic and conventional farms and to relate these parameters to fermentative parameters. The mean dry matter (DM) content was 364.10±93.31 and 424.70±95.93g/kg in the silage from organic and conventional farms respectively. The silage samples from organic farms had 17.00% higher (P≤ 0.05) tyramine (TY) than the silage from conventional farms. Conventional farm samples were characterized by 46.00% higher histamine (HIS) (P≤ 0.05), 9.80% higher putrescine (PUT) (P≤ 0.05), 17.30% higher cadaverine (CAD) (P≤ 0.05). Aflatoxins (AFL) (total) and zearalenone (ZEN), T-2/HT-2 concentrations were higher respectively 16.00% (P≤ 0.05) and 13.40% (P≤ 0.05), 1.80% (P≤ 0.05) in the silage prepared in organic farms. Deoxynivalenol (DON) concentration was higher 42.40% (P≤ 0.05) in silage from conventional farms. Volatile fatty acids (VFA), lactic acid, ethanol, pH and ammonia nitrogen showed that the silage samples from organic and conventional farms were of good quality. Our study suggests differences in biogenic amine formation or mycotoxins content in silage from organic and conventional farming, but, overall, the measured values are too low to be relevant for animal health. Furthermore, these differences might as well be due to the difference in dry matter content and plant maturity between the organic and conventional silage samples.(AU)

O objetivo do presente estudo foi investigar concentrações de aminas biogênicas micotoxinas em silagem embalada (principalmente gramíneas da família Poaceae) preparada em fazendas orgânicas e convencionais e relacionar esses parâmetros a parâmetros fermentativos. A massa seca média (MS) foi 364,10±93,31 e 424,70±95,93g/kg na silagem de fazendas orgânicas e convencionais, respectivamente. As amostras de silagem de fazendas orgânicas tinham 17% a mais de tyramina (TY) (p≤ 0,05) que as de fazendas convencionais. As amostras de fazendas convencionais foram caracterizadas por histamina (HIS) 46,00% mais alta (P≤ 0,05), 9,80% putrecina (PUT) mais alta (P≤ 0,05), 17,30% de cadaverina (CAD) mais alta (P≤ 0,05). Aflatoxnas (AFL) (total) e zearalenone (ZEN), T-2/HT-2 tinham concentrações mais altas em respectivamente 16,00% (P≤ 0,05) e 13,40% (P≤ 0,05), 1,80% (P≤ 0,05) na silagem preparada em fazendas orgânicas. Deoxinivalenol (DON) tinha concentração mais alta 42,40% (P≤ 0,05) na silagem de fazendas convencionais. Ácidos graxos voláteis (AGV), ácido lático, etanol, pH e nitrogênio de amônia mostraram que as amostras de silagem de fazendas orgânicas e convencionais tinham boa qualidade. Nosso estudo sugere diferenças na formação biogênica de amônia ou micotoxinas em silagem de fazendas orgânicas ou convencionais mas, em geral, os valores medidos foram muito baixos para serem relevantes à saúde animal. Ademais, essas diferenças podem ser devido à diferença na matéria sólida e maturidade da planta entre as amostras de silagem orgânica e convencional.(AU)
Descritores: Aminas Biogênicas/análise
Ácidos Graxos Voláteis
Micotoxinas/análise
Agricultura Orgânica
Poaceae
-Amônia/análise
Etanol/análise
Ácido Láctico/análise
Parâmetros de Referência
Responsável: BR68.1 - Biblioteca Virginie Buff D'Ápice


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Id: lil-769247
Autor: Tekeli, Ahmet; Yιldιz, Gültekin; Drochner, Winfried; Steingass, Herbert.
Título: Efficacy of essence oil supplementation to feeds on volatile fatty acid production / La eficacia de los suplementos de aceite de esencia en la producción de ácidos grasos volátiles
Fonte: Rev. MVZ Córdoba;20(supl.1):4884-4894, Dec. 2015. ilus, tab.
Idioma: en.
Resumo: Objective. Determine the effect of some plant extract supplementation to Total Mixed Ration (TMR), concentrate and hay on volatile fatty acid (VFA) production at 8 and 24 hours (h) using in vitro gas production technique in cattle. Material and methods. Three fistulated Holstein dairy cows were used for rumen fluid collection for application of in vitro gas production technique. Four essence oils (T. vulgaris, O. vulgare, S. aromaticum, Z. officinale) were used as plant extracts. Results. Essence oil supplementations to the examined feed groups had significant effect only on C2/C3 VFA level at 8 h in all feed groups (p<0.05). C2/C3 VFA level at 8 h significantly increased in the groups with Oregano 25 ppm supplementation for TMR and concentrate and in the groups with Thymol 25 ppm supplementation for hay. C3 VFA level at 8 h significantly increased in the group that received Syzygium 200 ppm supplementation for hay. Different plant extracts supplemented to TMR, concentrate and hay significantly affected C2, C3, IC4, IC5, C5 and C2/C3 VFA levels at 24 h (p<0.05). Conclusions. The findings of the study indicate that moderate doses of plant extracts result in increased VFA levels in ruminants while higher doses demonstrate the opposite effect.

Objetivo. Determinar el efecto de los suplementos de algunos extractos de plantas a Ración Total Mezclada (TMR), concentrado y heno sobre los ácidos grasos volátiles (AGV) a las 8 y 24 horas (h) utilizando la técnica de producción de gas in vitro en el ganado bovino. Material y métodos. Tres vacas lecheras Holstein fistuladas se utilizaron para la recogida de fluido ruminal y aplicar la técnica in vitro de producción de gas. Cuatro aceites esenciales (T. vulgaris, O. vulgare, S. aromaticum, Z. officinale) fueron utilizadas como extractos de plantas. Resultados. La suplementación de aceite esenciales a los grupos de alimentos estudiados tuvieron efecto significativo sólo en C2 / nivel C3 VFA a las 8 h en todos los grupos de alimentos (p<0.05). El C2 / nivel C3 VFA a las 8 h aumentó significativamente en los grupos con suplementación de orégano 25 ppm de la RTM y concentrado y en los grupos con suplementación de Timol a 25 ppm para el heno. El C3 nivel de VFA en 8 h aumentó significativamente en el grupo que recibió suplementación de Syzygium a razón de 200 ppm para el heno. Los diferentes extractos de plantas suplementadas con RTM, concentrado y heno afectó significativamente los niveles de C2, C3, IC4, IC5, C5 y C2/C3 VFA a las 24 h (p<0.05). Conclusiones. Los hallasgos del estudio indican que las dosis moderadas de extractos de plantas incrementan los niveles de AGV en rumiantes, mientras que dosis más altas producen el efecto contrario.
Descritores: Ácidos Graxos Voláteis
-Técnicas In Vitro
Extratos Vegetais
Responsável: CO332 - Facultad de Medicina



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