Base de dados : LILACS
Pesquisa : D10.477 [Categoria DeCS]
Referências encontradas : 5 [refinar]
Mostrando: 1 .. 5   no formato [Detalhado]

página 1 de 1

  1 / 5 LILACS  
              next record last record
seleciona
para imprimir
Fotocópia
Texto completo SciELO Brasil
Texto completo
Id: lil-797151
Autor: Tótoli, Eliane Gandolpho; Salgado, Hérida Regina Nunes.
Título: A green approach for the quantification of daptomycin in pharmaceutical formulation by UV spectrophotometry
Fonte: Braz. j. pharm. sci;51(4):811-821, Oct.-Dec. 2015. ilus, tab.
Idioma: en.
Resumo: Daptomycin is the first approved drug from a new class of antimicrobials, the cyclic lipopeptides, and is a very important antimicrobial agent in current clinical practice. Currently, there are no "green" analytical methods described in the literature to analyze the typical pharmaceutical dosage form of daptomycin. Thus, the aim of this work was to validate an environment-friendly spectrophotometric method in the UV region, for the analysis of daptomycin as a lyophilized powder. Water was used as diluent and the analyses were carried out on a spectrophotometer at 221 nm. The method met all validation requirements of the ICH guidelines, over a concentration range of 6-21 µg mL-1. A Student's t-test demonstrated that the proposed method was comparable to an HPLC method previously validated. Thus, the validated spectrophotometric method could quantify daptomycin in a powder form for injectable solutions, while being an economical, rapid, and "green" alternative for routine analysis in quality control...

A daptomicina é o primeiro membro aprovado de uma nova classe de antimicrobianos, os lipopeptídeos cíclicos, e é muito importante para a prática clínica atualmente. Não existem métodos analíticos "verdes" descritos na literatura para a análise da daptomicina na forma farmacêutica. Desta forma, o objetivo deste trabalho foi a validação de método espectrofotométrico na região do UV ambientalmente favorável para análise da daptomicina em pó liofilizado. A água foi escolhida como diluente e as análises foram realizadas em 221 nm. O método atendeu a todas as exigências de validação dos guias do ICH, na faixa de 6-21 µg mL-1. Teste t de Student mostrou que o método proposto é intercambiável com método de HPLC previamente validado. Assim, o método espectrofotométrico validado é capaz de quantificar a daptomicina em pó para solução injetável e é uma opção econômica, rápida e "verde" para análises de rotina do controle de qualidade deste fármaco...
Descritores: Daptomicina/análise
Espectrofotometria Ultravioleta/métodos
-Anti-Infecciosos/análise
Lipopeptídeos
Controle de Qualidade
Limites: Seres Humanos
Responsável: BR40.1 - DBD - Divisão de Biblioteca e Documentacão do Conjunto das Químicas


  2 / 5 LILACS  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo SciELO Brasil
Texto completo
Id: lil-742902
Autor: Lamari, Neuseli Marino.
Título: Effectiveness of a cardiac rehabilitation program during hospital stay / Efetividade de um programa reabilitação cardíaca durante a fase hospitalar
Fonte: Rev. bras. cir. cardiovasc = Braz. j. cardiovasc. surg. (impr.);30(1):VIII-IX, Jan-Mar/2015.
Idioma: en.
Descritores: Apoptose
Células Secretoras de Insulina/efeitos dos fármacos
Transdução de Sinais
/metabolismo
TOLL-LIKE RECEPTOR ABBREVIATIONS AS TOPIC/metabolismo
-Linhagem Celular Tumoral
/metabolismo
CASPASE ABATTOIRS/metabolismo
Fragmentação do DNA
Ativação Enzimática
Proteínas I-kappa B/metabolismo
Imunoprecipitação
Células Secretoras de Insulina/metabolismo
Proteínas Quinases JNK Ativadas por Mitógeno/metabolismo
Lipopeptídeos/farmacologia
/metabolismo
MYELOID DIFFERENTIATION FACTOR ACETATE KINASE/metabolismo
Palmitatos
Ligação Proteica
Fosfoproteínas/metabolismo
Poli(ADP-Ribose) Polimerases/metabolismo
Interferência de RNA
/genética
TOLL-LIKE RECEPTOR ABBREVIATIONS AS TOPIC/genética
/agonistas
TOLL-LIKE RECEPTOR TEMEFOS/agonistas
/genética
TOLL-LIKE RECEPTOR TEMEFOS/genética
/metabolismo
TOLL-LIKE RECEPTOR TEMEFOS/metabolismo
Limites: Animais
Ratos
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


  3 / 5 LILACS  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo SciELO Brasil
ALVES, Sydney Hartz
Texto completo
Id: lil-624029
Autor: Mario, Débora Alves Nunes; Denardi, Laura Bedin; Bandeira, Laíssa Arévalo; Antunes, Milene Silva; Santurio, Janio Morais; Severo, Luiz Carlos; Alves, Sydney Hartz.
Título: The activity of echinocandins, amphotericin B and voriconazole against fluconazole-susceptible and fluconazole-resistant Brazilian Candida glabrata isolates
Fonte: Mem. Inst. Oswaldo Cruz;107(3):433-436, May 2012. tab.
Idioma: en.
Resumo: The extensive use of azole antifungal agents has promoted the resistance of Candida spp to these drugs. Candida glabrata is a problematic yeast because it presents a high degree of primary or secondary resistance to fluconazole. In Brazil, C. glabrata has been less studied than other species. In this paper, we compared the activity of three major classes of antifungal agents (azoles, echinocandins and polyenes) against fluconazole-susceptible (FS) and fluconazole-resistant (FR) C. glabrata strains. Cross-resistance between fluconazole and voriconazole was remarkable. Among the antifungal agents, the echinocandins were the most effective against FS and FR C. glabrata and micafungin showed the lowest minimal inhibitory concentrations.
Descritores: Anfotericina B/farmacologia
Antifúngicos/farmacologia
Candida glabrata/efeitos dos fármacos
Equinocandinas/farmacologia
Fluconazol/farmacologia
Pirimidinas/farmacologia
Triazóis/farmacologia
-Candida glabrata/isolamento & purificação
Farmacorresistência Fúngica/efeitos dos fármacos
Lipopeptídeos/farmacologia
Testes de Sensibilidade Microbiana
Limites: Seres Humanos
Tipo de Publ: Estudo Comparativo
Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


  4 / 5 LILACS  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo SciELO Chile
Texto completo
Id: lil-612151
Autor: Cortés L, Jorge A; Russi N, July A.
Título: Equinocandinas / Echinocandins
Fonte: Rev. chil. infectol;28(6):529-536, dic. 2011. ilus, tab.
Idioma: es.
Resumo: The echinocandins, caspofugin, micafungin, and anidulafungin, are lipopeptides that inhibit fungal growth by binding to β - (1.3) d glucan synthase. This enzyme is responsible for the formation of the peptidoglycan cell wall, and it is essential in fungi such as Candida spp, but less important in the case of Aspergillus and Fusarium species. We review the history, pharmacology and clinical trials that have showed clinical efficacy similar to amphotericin B for the management of fungal infections such as candidemia, invasive candidiasis and aspergillosis, even in cases refractory to initial treatment. These drugs have less toxicity and discontinuation is uncommonly required. Despite similar spectrum and tolerability, there are several pharmacological differences. Only a few clinical trials compare the clinical efficacy between them and their clinical application cannot be generalized. However, the echinocandins have demonstrated clinical efficacy in patients with invasive candidiasis and in others forms of systemic mycoses.

Las equinocandinas -caspofugina, micafungina y ani-dulafungina- son lipopéptidos que inhiben el crecimiento fúngico al unirse a la β-(1,3) d glucano sintetasa, enzima esencial para la síntesis en la pared celular de hongos como Candida spp, y menos importante en el caso de especies de Aspergillus y Fusarium. Se revisa la historia, farmacología y los diferentes ensayos clínicos que han evidenciado similar eficacia clínica a la de anfotericina B para el manejo de infecciones micóticas como candidemia, candidiasis invasora y aspergilosis, inclusive en casos refractarios al manejo inicial. Estos medicamentos tienen menor toxicidad y en pocos casos hay necesidad de retiro del tratamiento. Dado su espectro y tolerabilidad similar, su farmacología permite diferenciarlas. Se dispone de información limitada de estudios clínicos que las comparen entre ellas, limitando la extrapolación de la información a todo el grupo. Sin embargo, presentan eficacia clínica comprobada en pacientes con varias micosis invasoras.
Descritores: Antifúngicos/farmacologia
Aspergillus/efeitos dos fármacos
Candida/efeitos dos fármacos
Equinocandinas/farmacologia
Lipopeptídeos/farmacologia
-Aspergillus/classificação
Ensaios Clínicos como Assunto
Candida/classificação
Testes de Sensibilidade Microbiana
Limites: Seres Humanos
Tipo de Publ: Research Support, Non-U.S. Gov't
Revisão
Responsável: BR1.1 - BIREME


  5 / 5 LILACS  
              first record previous record
seleciona
para imprimir
Fotocópia
Texto completo SciELO Brasil
Texto completo
Id: lil-556867
Autor: Fernandes, M. L; Mendes, M. E; Brunialti, M. K. C; Salomão, R.
Título: Human monocytes tolerant to LPS retain the ability to phagocytose bacteria and generate reactive oxygen species
Fonte: Braz. j. med. biol. res = Rev. bras. pesqui. méd. biol;43(9):860-868, Sept. 2010. ilus.
Idioma: en.
Resumo: Tolerance to lipopolysaccharide (LPS) occurs when animals or cells exposed to LPS become hyporesponsive to a subsequent challenge with LPS. This mechanism is believed to be involved in the down-regulation of cellular responses observed in septic patients. The aim of this investigation was to evaluate LPS-induced monocyte tolerance of healthy volunteers using whole blood. The detection of intracellular IL-6, bacterial phagocytosis and reactive oxygen species (ROS) was determined by flow cytometry, using anti-IL-6-PE, heat-killed Staphylococcus aureus stained with propidium iodide and 2',7'-dichlorofluorescein diacetate, respectively. Monocytes were gated in whole blood by combining FSC and SSC parameters and CD14-positive staining. The exposure to increasing LPS concentrations resulted in lower intracellular concentration of IL-6 in monocytes after challenge. A similar effect was observed with challenge with MALP-2 (a Toll-like receptor (TLR)2/6 agonist) and killed Pseudomonas aeruginosa and S. aureus, but not with flagellin (a TLR5 agonist). LPS conditioning with 15 ng/mL resulted in a 40 percent reduction of IL-6 in monocytes. In contrast, phagocytosis of P. aeruginosa and S. aureus and induced ROS generation were preserved or increased in tolerant cells. The phenomenon of tolerance involves a complex regulation in which the production of IL-6 was diminished, whereas the bacterial phagocytosis and production of ROS was preserved. Decreased production of proinflammatory cytokines and preserved or increased production of ROS may be an adaptation to control the deleterious effects of inflammation while preserving antimicrobial activity.
Descritores: Lipopeptídeos/farmacologia
Lipopolissacarídeos/farmacologia
Monócitos/imunologia
Pseudomonas aeruginosa/imunologia
Espécies Reativas de Oxigênio/metabolismo
Staphylococcus aureus/imunologia
-/imunologia
INTERLEUKIN-ABDOMEN, ACUTE/imunologia
Monócitos/efeitos dos fármacos
Monócitos/metabolismo
Fagocitose/imunologia
Pseudomonas aeruginosa/metabolismo
Espécies Reativas de Oxigênio/imunologia
Staphylococcus aureus/metabolismo
Receptores Toll-Like/antagonistas & inibidores
Limites: Adulto
Feminino
Seres Humanos
Masculino
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME



página 1 de 1
   


Refinar a pesquisa
  Base de dados : Formulário avançado   

    Pesquisar no campo  
1  
2
3
 
           



Search engine: iAH v2.6 powered by WWWISIS

BIREME/OPAS/OMS - Centro Latino-Americano e do Caribe de Informação em Ciências da Saúde