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Id: biblio-1087694
Autor: Tang, Jie; Chen, Ting-ting; Hu, Qiong; Lei, Dan; Sun, Qing; Zhang, Shu-ming; Zeng, Chao-yi; Zhang, Qing.
Título: Improved protease activity of Pixian broad bean paste with cocultivation of Aspergillus oryzae QM-6 and Aspergillus niger QH-3
Fonte: Electron. j. biotechnol;44:33-40, Mar. 2020. graf, tab, ilus.
Idioma: en.
Projeto: Transformation of Scientific and Technological Achievements Programs of Sichuan Province; . Natural Science Foundation of Sichuan Provincial Department of Education; . Chunhui Program of Ministry of Education of China; . Graduate Innovation Fund Project of Xihua University; . Application Foundation Project of Sichuan Provincial Department of Science and Technology.
Resumo: BACKGROUND: The preparation of broad bean koji is a key process in the production of Pixian broad bean paste (PBP). Protease is essential for the degradation of proteins during PBP fermentation. To obtain broad bean koji with high protease activity using the cocultivated strains of Aspergillus oryzae QM-6 (A. oryzae QM-6) and Aspergillus niger QH-3 (A. niger QH-3), the optimization of acid and neutral protease activities was carried out using Box­Behnken design with response surface methodology (RSM). RESULTS: The optimum conditions were found to be as follows: inoculation proportion (X1), 3:1 (A. oryzae QM-6: A. niger QH-3, w/w); culture temperature (X2), 33°C; inoculum size (X3), 0.5% (w/w); incubation time (X4), 5 d. The acid and neutral protease activities were 605.2 ± 12.4 U/g and 1582.9 ± 23.7 U/g, respectively, which were in good agreement with the predicted values. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis profiles revealed that the broad bean koji extracellular proteins in the case of cocultivation were richer compared to those in the case of A. oryzae QM-6 or A. niger QH-3 strain only. In addition, the free amino acids (FAAs) in the fermentation product were 55% higher in the cocultivation process than in that involving only A. oryzae QM-6, further confirming the diversity of proteases in the fermentation products. CONCLUSIONS: The optimal conditions of koji-making in PBP were obtained using RSM. The cocultivation of A. oryzae and A. niger increases the overall enzyme activities in the culture medium and the FAAs content, which would thus have potential application in the PBP industry.
Descritores: Peptídeo Hidrolases/metabolismo
Aspergillus niger
Aspergillus oryzae
Fabaceae/enzimologia
-Técnicas de Cocultura
Vicia faba
Eletroforese em Gel de Poliacrilamida
Fermentação
Aminoácidos
Responsável: CL1.1 - Biblioteca Central


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Id: lil-770136
Autor: Vanderhoof, Jon A.
Título: In time: misuse and overuse of amino acid formulas in cow milk allergy / Em tempo: uso indevido e excessivo de fórmulas de aminoácidos na alergia ao leite de vaca
Fonte: Rev. paul. pediatr;33(4):379-380, Oct.-Dec. 2015.
Idioma: pt.
Descritores: Hipersensibilidade a Leite/prevenção & controle
Fórmulas Infantis/química
Aminoácidos/administração & dosagem
Limites: Humanos
Animais
Feminino
Lactente
Bovinos
Tipo de Publ: Editorial
Responsável: BR1.1 - BIREME


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Id: biblio-886207
Autor: Lin, Sheng; Zhou, Guangli; Shao, Wei; Fu, Zhijian.
Título: Impact of dexmedetomidine on amino acid contents and the cerebral ultrastructure of rats with cerebral ischemia-reperfusion injury
Fonte: Acta cir. bras;32(6):459-466, June 2017. tab, graf.
Idioma: en.
Resumo: Abstract Purpose: To investigate the effects of dexmedetomidine (DEX) on amino acid contents and the cerebral ultrastructure of rats with cerebral ischemia-reperfusion injury (I/R). Methods: Thirty-six, male, Wistar rats were randomly divided into three groups: the sham operation group (group C), the ischemia-reperfusion group (group I/R), and the DEX group (group D). The middle cerebral artery occlusion model was prepared by the modified Longa method. The time of ischemia was 180 min, and 120 min after reperfusion, the amount of glutamate (Glu), and γ-aminobutyric acid (GABA) in the brain were measured, and the ultrastructure-level changes in the cerebral cortex were examined using electron microscopy. Results: Compared to group C, Glu contents in group D, and I/R significantly increased. Compared to group I/R, Glu contents in group D significantly decreased. Compared to group C, GABA contents in group D, and I/R significantly increased, and those in group D significantly increased, as compared to group I/R. The cerebral ultrastructure was normal in group C. Vacuolar degeneration in the plastiosome and nervous processes, was more critical than in group D. Vascular endothelial cells (VEC) were damaged. On the contrary, these changes in group D significantly improved. Conclusion: Dexmedetomidine is capable of decreasing glutamergic content, and increasing GABAergic content, in order to decrease the injury of the cerebral ultrastructure, following cerebral ischemia-reperfusion injury.
Descritores: Traumatismo por Reperfusão/metabolismo
Córtex Cerebral/química
Isquemia Encefálica/tratamento farmacológico
Fármacos Neuroprotetores/farmacologia
Dexmedetomidina/farmacologia
Glutamina/metabolismo
-Córtex Cerebral/ultraestrutura
Isquemia Encefálica/metabolismo
Ratos Wistar
Ácido gama-Aminobutírico/efeitos dos fármacos
Ácido gama-Aminobutírico/metabolismo
Aminoácidos/efeitos dos fármacos
Aminoácidos/metabolismo
Limites: Animais
Masculino
Ratos
Responsável: BR1.1 - BIREME


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Id: biblio-1066527
Autor: Almeida, Maria Elisa Wohlers de(edt); Simão, Antonia Mattos(edt).
Título: Aminoácidos de hidrolisados de proteína de soja dosagem de ácido glutâmico / Detection of aminoacids in protein hydrolysates of soybean determination of glutamic acid
Fonte: Rev. Inst. Adolfo Lutz;32(Único):105-112, 1972. graf, ilus.
Idioma: pt.
Resumo: Cromotografia bi-dimensional em papel foi aplicada com a finalidade de separar os diferentes aminoácidos de hidrolisado de proteínas de soja. Foi introduzido, com resultados satisfatório , um novo artifício para identificação dos aminoácidos, e foram feitas algumas modificações na determinação do ácido glutâmico. As manchas correspondentes ao ácidos glutâmico (padrão e amostra) foram recortadas, eluídas e o ácido glutâmico foi determinado por espectrofotometria.
Descritores: Alimentos de Soja
Aminoácidos
Ácido Glutâmico
Responsável: BR76.1 - Biblioteca


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Id: biblio-974282
Autor: Santos, Alexandra de Andrade; Silveira, Joaquim Albenísio Gomes da; Guilherme, Eliezer de Araujo; Bonifacio, Aurenivia; Rodrigues, Artenisa Cerqueira; Figueiredo, Márcia do Vale Barreto.
Título: Changes induced by co-inoculation in nitrogen-carbon metabolism in cowpea under salinity stress
Fonte: Braz. j. microbiol;49(4):685-694, Oct.-Dec. 2018. tab, graf.
Idioma: en.
Resumo: ABSTRACT To mitigate the deleterious effects of abiotic stress, the use of plant growth-promoting bacteria along with diazotrophic bacteria has been increasing. The objectives of this study were to investigate the key enzymes related to nitrogen and carbon metabolism in the biological nitrogen fixation process and to elucidate the activities of these enzymes by the synergistic interaction between Bradyrhizobium and plant growth-promoting bacteria in the absence and presence of salt stress. Cowpea plants were cultivated under axenic conditions, inoculated with Bradyrhizobium and co-inoculated with Bradyrhizobium sp. and Actinomadura sp., Bradyrhizobium sp. and Bacillus sp., Bradyrhizobium sp. and Paenibacillus graminis, and Bradyrhizobium sp. and Streptomycessp.; the plants were also maintained in the absence (control) and presence of salt stress (50 mmolL-1 NaCl). Salinity reduced the amino acids, free ammonia, ureides, proteins and total nitrogen content in nodules and increased the levels of sucrose and soluble sugars. The co-inoculations responded differently to the activity of glutamine synthetase enzymes under salt stress, as well as glutamate synthase, glutamate dehydrogenase aminating, and acid invertase in the control and salt stress. Considering the development conditions of this experiment, co-inoculation with Bradyrhizobium sp. and Bacillus sp. in cowpea provided better symbiotic performance, mitigating the deleterious effects of salt stress.
Descritores: Carbono/metabolismo
Cloreto de Sódio/metabolismo
Vigna/metabolismo
Nitrogênio/metabolismo
-Microbiologia do Solo
Cloreto de Sódio/análise
Actinobacteria/fisiologia
Raízes de Plantas/crescimento & desenvolvimento
Raízes de Plantas/metabolismo
Raízes de Plantas/microbiologia
Bradyrhizobium/fisiologia
Inoculantes Agrícolas/fisiologia
Vigna/crescimento & desenvolvimento
Vigna/microbiologia
FRONTAL LOBE0
Aminoácidos/metabolismo
Fixação de Nitrogênio
Responsável: BR1.1 - BIREME


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Id: biblio-1046023
Autor: Lopes, Matheus Gomes; Dominguez, José Henrique Echenique; Corrêa, Marcio Nunes; Schmitt, Eduardo; Fischer, Geferson.
Título: Rumen-protected methionine in cattle: influences on reproduction, immune response, and productive performance / Metionina protegida da degradação ruminal em bovinos: influências na reprodução, resposta imune e desempenho produtivo
Fonte: Arq. Inst. Biol;86:e1292018, 2019.
Idioma: en.
Resumo: Nowadays, information and knowledge generated about the main ingredients used in cattle diets have enabled greater assertiveness in their formulation. Among the ingredients, amino acids stand out as a promising tool, capable of reducing total crude protein (CP) levels and adjusting optimal metabolizable protein concentrations in diets. Nutritional programs allow diets due to amino acid requirements, providing the opportunity to increase the efficiency of the use of dietary nitrogen, increasing the scarce protein concentrates, maintaining or even boosting performance. This review aimed to present the influences of methionine, in its formulation protected from ruminal degradation, according to reproductive parameters, immune response, and productive performance in cattle. Reproduction-related benefits are linked to the early days of embryonic implantation in the uterine environment, which initially secretes through the histotroph produced by endometrial glands, the nutrients needed to develop the conceptus until implantation, and vascular communication with the mother. Given the immune response, the main results are related to the benefits of innate immunity, with marked increase in phagocytic capacity of neutrophils and monocytes, as well as an important antioxidant effect mediated by methionine products, such as glutathione. When evaluating the influences on productivity, the most evident correlation is the increase in protein in the milk of cows supplemented with methionine. Over the past decade, studies investigating the potential benefits of this strategic supplementation in beef cattle were intensified, expanding the opportunities for the development of new experimental projects.(AU)

Atualmente, as informações e o conhecimento gerado sobre os principais ingredientes utilizados em dietas para bovinos possibilitaram maior assertividade em suas formulações. Dentre esses ingredientes, os aminoácidos se destacam como uma ferramenta promissora, capaz de levar à redução nos níveis totais de proteína bruta e ajustar as concentrações ideais de proteína metabolizável nas dietas. Programas nutricionais permitem formular dietas por exigências de aminoácidos, oportunizando o aumento na eficiência de utilização do nitrogênio dietético, reduzindo dispêndios com concentrados ricos em proteína, com a manutenção, ou ainda, incremento de desempenho. Esta revisão buscou apresentar as influências da metionina, em sua formulação protegida da degradação ruminal, frente a parâmetros reprodutivos, resposta imune e desempenho produtivo em bovinos. Os benefícios relacionados à reprodução se mostram ligados aos primeiros dias de implantação embrionária no ambiente uterino, que inicialmente secreta, através do histotrofo produzido por glândulas endometriais, os nutrientes necessários para o desenvolvimento do concepto até a implantação e comunicação vascular com a mãe. Dada a resposta imune, os principais resultados estão relacionados aos benefícios da imunidade inata, com aumento acentuado da capacidade fagocitária de neutrófilos e monócitos, assim como um importante efeito antioxidante mediado por produtos originários da metionina, como a glutationa. Por fim, quando avaliadas as influências em produtividade, a correlação mais evidente é o incremento em proteína no leite de vacas suplementadas com metionina. Na última década, os estudos que investigam os potenciais benefícios dessa suplementação estratégica em bovinos de corte foram intensificados, abrindo um caminho de oportunidades para o desenvolvimento de novos projetos experimentais.(AU)
Descritores: Bovinos
Aminoácidos
-Metionina
Antioxidantes
Limites: Animais
Tipo de Publ: Revisão
Responsável: BR1942.1 - NID - Biblioteca - Núcleo de Informação e Documentação


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Id: lil-60565
Autor: Cavalheiro, Esper Abräo, ed; Lehmann, John, ed; Turski, Kechoslaw, ed.
Título: Frontiers in excitatory amino acid research.
Fonte: s.l; Alan R. Liss; 1988. 745 p. ilus. (Neurology and neurobiology, 46).
Idioma: en.
Conferência: Apresentado em: International Symposium Escitatory Amino Acids'88, Manaus, Mar. 28-Apr. 2, 1988.
Descritores: Aminoácidos
-Congresso
Receptores de Neurotransmissores
Responsável: BR1.1 - BIREME
BR1.2/QU60*C376f,1988


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Id: lil-633097
Autor: Malaver Ortega, Luis Fernando; Alméciga-Díaz, Carlos Javier; Morales Monsalve, Inés Stella; Echeverri Peña, Olga Yaneth; Guevara Morales, Johana; Zuluaga Torres, Estefania; Córdoba Ruiz, Henry Alírio; Barrera Avellaneda, Luis Alejandro.
Título: Cuantificación de aminoácidos en plasma empleando Cromatografía Líquida de Alta Eficiencia / Plasma amino acid quantification using High Performance Liquid Chromatography
Fonte: Acta bioquím. clín. latinoam;43(4):647-661, oct.-dic. 2009. graf, tab.
Idioma: es.
Projeto: Pontificia Universidad Javeriana. ID PPTA 00000322, ID PRY 000335.
Resumo: Las aminoacidopatías son errores innatos del metabolismo intermediario de los aminoácidos. Su confirmación diagnóstica y seguimiento se realiza con la cuantificación de aminoácidos libres en fluidos biológicos por técnicas como la cromatografía líquida de alta eficiencia (HPLC), para lo que es necesario comparar con valores de referencia normales. La población colombiana no cuenta con estos valores disponibles y el diagnóstico es realizado por comparación con los de otras poblaciones. En el presente trabajo se obtuvieron valores de referencia de aminoácidos en plasma en una población de niños (n=36) y adultos no afectados (n=17), mediante HPLC por derivatización postcolumna con ninhidrina. Los valores de referencia obtenidos fueron ligeramente más elevados que los informados para otras poblaciones y permitieron la identificación de doce casos de aminoacidopatías, incluyendo fenilcetonuria clásica, hiperfenilalaninemia, hiperglicinemia no cetósica, desórdenes del ciclo de la urea, tirosinemia. La implementación de la cuantificación de aminoácidos por HPLC y la obtención de los valores de referencia de aminoácidos en plasma permitirán aumentar el conocimiento sobre la incidencia de las aminoacidopatías en el país para garantizar, junto con otros factores, su diagnóstico preciso y oportuno y la implementación de un adecuado seguimiento nutricional.

Aminoacidopathies are inborn errors of the amino acid intermediary metabolism. The benchmark method used for their diagnosis and monitoring is the quanti!cation of free amino acids in biological fluids using High Performance Liquid Chromatography (HPLC), which needs to be compared against normal reference values. However, those amino acid reference values are not available for the Colombian population and the diagnosis is usually made using values from American or European populations. In this work, plasma amino acid reference values in non-affected children (n=36) and adults (n=17) were established, using an HPLC method with a postcolumn derivatization with ninhidrine. Plasma amino acid reference values in a Colombian population were slightly higher compared with those reported for other populations, and enabled the identification of twelve aminoacidopathies including urea cycle disorders, phenylketonuria, hyperphenylalaninemia, nonketotichyperglycinemia, hepatorrenaltyrosinemia and maple syrup urine disease. The implementation of amino acid cuantification by HPLC and the construction of plasma amino acid reference values is very useful for a suitable and precise diagnosis of amino acid disorders, the implementation of proper nutritional treatments, and an increased knowledge of aminoacidopathy incidence in Colombia.
Descritores: Erros Inatos do Metabolismo dos Aminoácidos/diagnóstico
Erros Inatos do Metabolismo dos Aminoácidos/sangue
Aminoácidos/metabolismo
-Valores de Referência
Cromatografia Líquida
Aminoácidos/sangue
Limites: Humanos
Masculino
Feminino
Recém-Nascido
Lactente
Adulto
Pessoa de Meia-Idade
Tipo de Publ: Estudo Comparativo
Responsável: AR1.2 - Instituto de Investigaciónes Epidemiológicas


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Id: biblio-889154
Autor: Shen, Jian Ying; Liao, Jin Zhi; Guo, Li Li; Su, Rui Fang.
Título: Effects of monosulfuron-ester on metabolic processes of nitrogen-fixing cyanobacteria Anabaena flos-aquae and Anabaena azotica
Fonte: Braz. j. microbiol;48(3):544-550, July-Sept. 2017. tab, graf.
Idioma: en.
Projeto: National Natural Science Foundation of China.
Resumo: Abstract Presence of the relatively new sulfonylurea herbicide monosulfuron-ester at 0.03-300 nmol/L affected the growth of two non-target nitrogen-fixing cyanobacteria (Anabaena flos-aquae and Anabaena azotica) and substantially inhibited in vitro Acetolactate synthase activity, with IC50 of 3.3 and 101.3 nmol/L for A. flos-aquae and A. azotica, respectively. Presenting in 30-300 nmol/L, it inhibited protein synthesis of the cyanobacteria with less amino acids produced as its concentration increased. Our findings support the view that monosulfuron-ester toxicity in both nitrogen-fixing cyanobacteria is due to its interference with protein metabolism via inhibition of branch-chain amino acid biosynthesis, and particularly Acetolactate synthase activity.
Descritores: Pirimidinas/toxicidade
Compostos de Sulfonilureia/toxicidade
Anabaena/efeitos dos fármacos
Anabaena/metabolismo
Anabaena flos-aquae/efeitos dos fármacos
Anabaena flos-aquae/metabolismo
Ésteres/toxicidade
Herbicidas/toxicidade
Fixação de Nitrogênio/efeitos dos fármacos
-Anabaena/genética
Anabaena flos-aquae/genética
Aminoácidos/metabolismo
Nitrogênio/metabolismo
Responsável: BR1.1 - BIREME


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Id: biblio-1017075
Autor: Yu, Xiao-Chun; Ma, Shi-Liang; Xu, Yan; Fu, Cheng-Hao; Jiang, Chun-Ying; Zhou, Chen-Yu.
Título: Construction and application of a novel genetically engineered Aspergillus oryzae for expressing proteases
Fonte: Electron. j. biotechnol;29:32-38, sept. 2017. tab, ilus, graf.
Idioma: en.
Resumo: Background: We aimed to test the possibility of improving polypeptide production from soybean meal fermentation by engineered Aspergillus oryzae strains. Four different protease genes were cloned and transformed into wild-type A. oryzae, and the engineered A. oryzae strains were then used for soybean meal fermentation. Results: The results showed different degrees of improvement in the protease activity of the four transformants when compared with wild-type A. oryzae. A major improvement in the polypeptide yield was achieved when these strains were used in soybean meal fermentation. The polypeptide conversion rate of one of the four transformants, A. oryzae pep, reached 35.9%, which was approximately twofold higher than that exhibited by wild-type A. oryzae. Amino acid content analysis showed that the essential amino acid content and amino acid composition of the fermentation product significantly improved when engineered A. oryzae strains were used for soybean meal fermentation. Conclusions: These findings suggest that cloning of microbial protease genes with good physicochemical properties and expressing them in an ideal host such as A. oryzae is a novel strategy to enhance the value of soybean meal.
Descritores: Peptídeo Hidrolases/metabolismo
Aspergillus oryzae/enzimologia
Aspergillus oryzae/genética
-Peptídeo Hidrolases/genética
Soja
Transformação Genética
Engenharia Genética
Clonagem Molecular
Fermentação
Farinha
Aminoácidos/análise
Responsável: CL1.1 - Biblioteca Central



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