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Texto completo SciELO Chile
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Id: biblio-1124877
Autor: Chaiyamoon, Arada; Bunsueb, Sudtida; Iamsaard, Sitthichai.
Título: Changes of tyrosine phosphorylation in liver and kidney of polycystic ovarian rats induced by letrozole / Cambios de fosforilación de tirosina en hígado y riñón de ratas ováricas poliquísticas inducidas por letrozol
Fonte: Int. j. morphol;38(4):919-923, Aug. 2020. tab, graf.
Idioma: en.
Resumo: Letrozole (Letro) is a drug commonly used for breast cancer treatment since it can decrease estrogen level. In experimental animal, the Letro has been used to induce the polycystic ovarian syndrome (PCOS) model. Tyrosine phosphorylation (TyrPho) is an essential process in various biological functions both normal and abnormal conditions especially reproduction. Although some side effects of Letro are reported, the alterations of TyrPho responsible for liver and kidney functions have never been demonstrated. In this study, the blood serum, liver, and kidney of control and PCOS rats induced with Letro (orally, 1 mg/ KgBW) for consecutive 21 days were used to determine the serum biochemical components and to investigate the TyrPho expression using western blot analysis. Histopathology of such tissues was observed by Masson's trichrome staining. The results showed that Letro did not affect histological structures but significantly increased the serum levels of urea nitrogen, cholesterol, triglyceride, HDL, LDL, ALT, AST, and alkaline phosphatase. Additionally, the TyrPho protein expressions of 32 and 27 kDas in liver and of 55 and 43 kDas in kidney were increased while of a kidney 26 kDa was decreased as compared to those of control. In conclusion, this recent study indicated that the changes of TyrPho proteins in liver and kidney induced with Letro associated with their functions by alteration of serum biochemical levels.

El letrozol (Letro) es un medicamento utilizado comúnmente para el tratamiento del cáncer de mama, debido a que puede disminuir el nivel de estrógeno. En animales de experimentación, el Letro se ha utilizado para inducir el modelo de síndrome de ovario poliquístico (PCOS). La fosforilación de tirosina (TyrPho) es un proceso esencial en diversas funciones biológicas, tanto en condiciones normales como anormales, especialmente en la reproducción. A pesar de informes que indican algunos efectos secundarios de Letro, no se han demostrado las alteraciones de TyrPho responsables de las funciones hepáticas y renales. En este estudio, el suero sanguíneo, el hígado y el riñón control y las ratas PCOS inducidas con Letro (por vía oral, 1 mg / KgBW) durante 21 días consecutivos se usaron para determinar los componentes bioquímicos del suero y para investigar la expresión de TyrPho usando análisis de transferencia Western. La histopatología de los tejidos se observó mediante la tinción tricrómica de Masson. Los resultados mostraron que Letro no afectó las estructuras histológicas, pero aumentó significativamente los niveles séricos de urea, colesterol, triglicéridos, HDL, LDL, ALT, AST y fosfatasa alcalina. Además, las expresiones de la proteína TyrPho de 32 y 27 kDas en el hígado y de 55 y 43 kDas en el riñón aumentaron mientras que en un riñón disminuyeron 26 kDa en comparación con el control. En conclusión, este estudio indicó que los cambios de las proteínas TyrPho en el hígado y los riñones inducidos con Letro se asociaron con sus funciones mediante la alteración de los niveles bioquímicos en suero.
Descritores: Síndrome do Ovário Policístico/induzido quimicamente
Letrozol/efeitos adversos
Rim/efeitos dos fármacos
Fígado/efeitos dos fármacos
-Fosforilação/fisiologia
Tirosina/metabolismo
Western Blotting
Ratos Wistar
Modelos Animais de Doenças
Eletroforese em Gel de Poliacrilamida
Limites: Animais
Feminino
Ratos
Responsável: CL1.1 - Biblioteca Central


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Aldrighi, José Mendes
Abdalla, Dulcineia Saes Parra
Texto completo
Id: lil-334405
Autor: Pereira, Isabela Rosier Olimpio; Bertolami, Marcelo Chiara; Faludi, André Arpad; Campos, Maria Fernanda; Ferderbar, Simone; Lima, Emersom Silva; Aldrighi, José Mendes; Abdalla, Dulcineia Saes Parra.
Título: Peroxidação lipídica e inativação do óxido nítrico na pós-menopausa / Lipid peroxidation and nitric oxide inactivation in postmenopausal women
Fonte: Arq. bras. cardiol;80(4):406-423, Apr. 2003. graf.
Idioma: pt; en.
Resumo: OBJECTIVE: To assess the effect of endogenous estrogens on the bioavailability of nitric oxide ( NO) and in the formation of lipid peroxidation products in pre- and postmenopausal women. METHODS: NOx and S-nitrosothiols were determined by gaseous phase chemiluminescence, nitrotyrosine was determined by ELISA, COx (cholesterol oxides) by gas chromatography, and cholesteryl linoleate hydroperoxides (CE18:2-OOH), trilinolein (TG18:2-OOH), and phospholipids (PC-OOH) by HPLC in samples of plasma. RESULTS: The concentrations of NOx, nitrotyrosine, COx, CE18:2-OOH, and PC-OOH were higher in the postmenopausal period (33.8±22.3 mM; 230±130 nM; 55±19 ng/mL; 17±8.7 nM; 2775±460 nM, respectively) as compared with those in the premenopausal period (21.1±7.3 mM; 114±41 nM; 31±13 ng/mL; 6±1.4 nM; 1635±373 nM). In contrast, the concentration of S-nitrosothiols was lower in the postmenopausal period (91±55 nM) as compared with that in the premenopausal p in the premenopausal period (237±197 nM). CONCLUSION: In the postmenopausal period, an increase in nitrotyrosine and a reduction of S-nitrosothiol formation, as well as an increase of COx, CE18:2-OOH and PC-OOH formation occurs. Therefore, òNO inactivation and the increase in lipid peroxidation may contribute to endothelial dysfunction and to the greater risk for atherosclerosis in postmenopausal women
Descritores: Peroxidação de Lipídeos
Pós-Menopausa
Estrogênios
Óxido Nítrico
-Arteriosclerose
Tirosina
Vasodilatação
Colesterol
Pré-Menopausa
Pós-Menopausa
Estradiol
Óxido Nítrico
S-Nitrosotióis/sangue
Limites: Humanos
Feminino
Adulto
Pessoa de Meia-Idade
Responsável: BR1.1 - BIREME


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Id: lil-461270
Autor: Luchs, Adriana.
Título: Transdução de sinal: um olhar sobre a insulina / Signal transduction: an overview on insulin
Fonte: Rev. Inst. Adolfo Lutz;65(3):157-164, set.-dez. 2006. ilus.
Idioma: pt.
Resumo: Os esforços de muitos laboratórios têm concentrado no desenvolvimento de pesquisas e na descoberta de vias moleculares que atuam na mediação da resposta pleiotrópica da insulina. Os estudos sobre o mecanismo de ação insulínico levaram a descoberta do receptor tirosina quinase e várias proteínas ligantes que são diretamente ativadas por meio de sítios de tirosinas fosforiladas existentes nesses receptores. A família dos substratos do receptor de insulina (IRSs) são as principais proteínas envolvidas na transdução do sinal intracelular desencadeado pela insulina as quais são encontradas em uma grande variedade de células e tecidos. Esse trabalho de revisão versa sobre o tema referente ao complexo do receptor de insulina e a cascata de sinalização induzida por esse hormônio.
Descritores: Insulina
Receptor de Insulina
Sistemas do Segundo Mensageiro
Tirosina
Transdução de Sinais
Responsável: BR91.2 - Centro de Documentação


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Id: biblio-1054941
Autor: Sahin, Suzan; Oncel, Mehmet Y; Bidev, Duygu; Okur, Nilufer; Talim, Beril; Oguz, Serife S.
Título: Miopatía nemalínica tratada con L-tirosina para aliviar los síntomas en un recién nacido / Nemaline rod myopathy treated with L-tyrosine to relieve symptoms in a neonate
Fonte: Arch. argent. pediatr;117(4):382-386, ago. 2019. ilus.
Idioma: en; es.
Resumo: La miopatía nemalínica es un trastorno heterogéneo definido por la presencia de estructuras con forma de bastones, conocidas como cuerpos nemalínicos (o bastones de nemalina). El diagnóstico se funda en la debilidad muscular, además de la visualización de cuerpos nemalínicos en la biopsia muscular. La miopatía nemalínica no tiene cura. Las estrategias terapéuticas para este trastorno son sintomáticas y empíricas. En este artículo, presentamos el caso de una recién nacida con insuficiencia respiratoria grave y debilidad muscular generalizada, a la que se le diagnosticó miopatía nemalínica a través de la biopsia muscular. La paciente tuvo una notable disminución de la sialorrea y una mejora de los movimientos espontáneos después del tratamiento con L-tirosina. Este caso se presenta para destacar la importancia de la biopsia muscular en el diagnóstico diferencial de la hipotonía grave durante el período neonatal y el posible beneficio del aporte suplementario de L-tirosina para disminuir la sialorrea y restaurar la fuerza muscular.

Nemaline myopathy (NM) is a heterogeneous disorder defined by the presence of rod-shaped structures known as nemaline bodies or rods. The diagnosis is based on muscle weakness, combined with visualization of nemaline bodies on muscle biopsy. There is no curative treatment for nemaline myopathy. Therapeutic strategies for this condition are symptomatic and empirical. Herein, we present a newborn with severe respiratory failure and generalized muscle weakness, who was diagnosed as NM by muscle biopsy. The patient experienced remarkable decrease in sialorrhea and improvement of spontaneous movements after L-tyrosine treatment. This case is presented to emphasize the importance of muscle biopsy in the differential diagnosis of severe hypotonia during neonatal period and a possible benefit of L-tyrosine supplementation for decreasing sialorrhea and restoring muscle strength.
Descritores: Tirosina/uso terapêutico
Miopatias da Nemalina/diagnóstico
-Biópsia
Miopatias da Nemalina/terapia
Evolução Fatal
Hipotonia Muscular
Limites: Humanos
Feminino
Recém-Nascido
Tipo de Publ: Relatos de Casos
Responsável: AR94.1 - Centro de Información Pediatrica


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Id: biblio-1040154
Autor: Chaichun, Amnart; Burawat, Jaturon; Arun, Supatcharee; Tongpan, Saranya; Kanla, Pipatpong; Sawatpanich, Tarinee; Iamsaard, Sitthichai.
Título: Mimosine increases the expressions of tyrosine phosphorylated protein in mouse seminal vesicles / La mimosina aumenta la expresión de la proteína tirosina fosforilada en las vesículas seminales del ratón
Fonte: Int. j. morphol;37(4):1463-1468, Dec. 2019. graf.
Idioma: en.
Projeto: Faculty of Medicine, Khon Kaen University, Thailand.
Resumo: Acute effect of purified mimosine (MiMo) extracted from Leucaena leucocephala on testicular histopathology has been documented with seminal vesicle (SV) atrophy. Since protein phosphorylation and seminal secretions play important roles in sperm physiology, this study aimed to study the alteration of substances including tyrosine phosphorylated (TyrPho) proteins in seminal vesicle treated with MiMo. Male mice were divided into a control and experimental groups treated with purified MiMo at 3 doses of 15, 30, and 60 mg/KgBW, respectively for 35 consecutive days. The morphology and weights of SV were compared among groups. The levels of magnesium and fructosamine in SV fluid were assayed. The profiles of equally SV total proteins were compared using SDS-PAGE. The expression of seminal TyrPho proteins was detected by western blotting. Recent results showed the decreased weights of SV in MiMo treated mice compared to control. However MiMo in all doses did not affect the levels of magnesium and fructosamine in SV fluid. The SV protein expression of 130 and 55 kDas was obviously decreased in a high dose MiMo. In dose-dependent response, the expressions of 72 and 55 kDas TyrPho proteins of SV were increased. In conclusion, MiMo could affect SV morphological size and protein secretions especially TyrPho proteins.

El efecto agudo de la mimosina purificada (MiMo) extraída de Leucaena leucocephala en la histopatología testicular se ha documentado con atrofia de vesícula seminal (VS). Debido a que la fosforilación de proteínas y las secreciones seminales tienen un papel importante en la fisiología de los espermatozoides, este estudio tuvo como objetivo estudiar la alteración de sustancias como la proteína tirosina fosforilada (TyrPho) en vesículas seminales tratadas con MiMo. Los ratones se dividieron en un grupo control y un grupo experimental y se trataron con MiMo purificado en 3 dosis de 15, 30 y 60 mg / KgBW, respectivamente, durante 35 días seguidos. La morfología y los pesos de VS se compararon entre los grupos. Fueron analizados los niveles de magnesio y fructosamina en el fluido VS. Los perfiles de las proteínas totales de VS se compararon utilizando SDS-PAGE. La expresión de la proteína TyrPho en las vesículas seminales se detectó mediante transferencia de Western blot. Los resultados recientes muestran la disminución del peso de las VS en ratones tratados con MiMo, en comparación con el grupo control. Sin embargo, en ninguna de las dosis se vieron afectados por mimosina purificada los niveles de magnesio y fructosamina en el líquido de las VS. La expresión de la proteína en VS de 130 y 55 kDas disminuyó notablemente en una dosis alta de MiMo. En la respuesta dependiente de la dosis, aumentaron las expresiones de 72 y 55 kDas de las proteínas TyrPho en las VS. En conclusión, la mimosina purificada podría afectar el tamaño morfológico de las VS y la expresión de proteínas, especialmente las proteínas TyrPho.
Descritores: Fosfoproteínas/efeitos dos fármacos
Glândulas Seminais/efeitos dos fármacos
Mimosina/administração & dosagem
-Tamanho do Órgão
Fosfoproteínas/metabolismo
Fosforilação
Glândulas Seminais/patologia
Tirosina/análogos & derivados
Western Blotting
Fosfotirosina
Eletroforese em Gel de Poliacrilamida
Camundongos Endogâmicos ICR
Mimosina/farmacologia
Limites: Animais
Masculino
Camundongos
Responsável: CL1.1 - Biblioteca Central


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Id: biblio-1016090
Autor: Castro, Daniela E; Murguía-Romero, Miguel; Thomé, Patricia E; Peña, Antonio; Calderón-Torres, Marissa.
Título: Putative 3-nitrotyrosine detoxifying genes identified in the yeast Debaryomyces hansenii: in silico search of regulatory sequences responsive to salt and nitrogen stress
Fonte: Electron. j. biotechnol;29:1-6, sept. 2017. graf, tab.
Idioma: en.
Projeto: DGAPA-UNAM.
Resumo: Background: During salt stress, the yeast Debaryomyces hansenii synthesizes tyrosine as a strategy to avoid the oxidation of proteins. Tyrosine reacts with nitrogen radicals to form 3-nitrotyrosine. 3-nitrotyrosine prevents the effects of associated oxidative stress and thus contributes to the high halotolerace of the yeast. However, the mechanism of how D. hansenii counteracts the presence of this toxic compound is unclear. In this work, we evaluated D. hansenii's capacity to assimilate 3-nitrotyrosine as a unique nitrogen source and measured its denitrase activity under salt stress. To identify putative genes related to the assimilation of 3-nitrotyrosine, we performed an in silico search in the promoter regions of D. hansenii genome. Results: We identified 15 genes whose promoters had binding site sequences for transcriptional factors of sodium, nitrogen, and oxidative stress with oxidoreductase and monooxygenase GO annotations. Two of these genes, DEHA2E24178g and DEHA2C00286g, coding for putative denitrases and having GATA sequences, were evaluated by RT-PCR and showed high expression under salt and nitrogen stress. Conclusions: D. hansenii can grow in the presence of 3-nitrotyrosine as the only nitrogen source and has a high specific denitrase activity to degrade 3-nitrotyrosine in 1 and 2 M NaCl stress conditions. The results suggest that given the lack of information on transcriptional factors in D. hansenii, the genes identified in our in silico analysis may help explain 3-nitrotyrosine assimilation mechanisms.
Descritores: Tirosina/análogos & derivados
Tirosina/metabolismo
Debaromyces/genética
Debaromyces/metabolismo
-Tirosina/genética
Transcrição Genética
Leveduras
Sequências Reguladoras de Ácido Nucleico
Regiões Promotoras Genéticas
Estresse Oxidativo
Reação em Cadeia da Polimerase em Tempo Real
Osmorregulação
Extremófilos
FRONTAL LOBE0
Nitrogênio/metabolismo
Responsável: CL1.1 - Biblioteca Central


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Id: biblio-954194
Autor: Sawatpanich, Tarinee; Arun, Supatcharee; Tongpan, Saranya; Chaichun, Amnart; Sampannang, Apichakarn; Sukhorum, Wannisa; Maneenin, Chanwit; Burawat, Jaturon; Iamsaard, Sitthichai.
Título: Localization and changes of tyrosine phosphorylated proteins and ß actin in epididymis of rats treated with valproic acid / Localización y cambios de las proteínas tirosina fosforiladas y la ß actina en epidídimos de ratas tratadas con ácido valproico
Fonte: Int. j. morphol;36(3):835-840, Sept. 2018. graf.
Idioma: en.
Projeto: Khon Kaen University.
Resumo: Tyrosine phosphorylated proteins have been localized and identified in male reproductive tissues such as testis and capacitated/ acrosome reacted sperm except epididymis. The changes of such proteins are associated with decreased sperm quality of valproic acid treatment. This study aimed to investigate the presence and alterations of protein phosphorylation in epididymal epithelium and fluid of rats treated VPA. Sixteen adult male rats were divided into control and VPA-treated groups (n=8/ each). Treated rats were injected with VPA (500 mg/ kgBW, intraperitoneally) for 10 consecutive days. At the end of experiment, the monoclonal antiphosphotyrosine (clone 4G10) was used for immunohistochemistry to probe tyrosine phosphorylated proteins and also to examine the expression of such proteins using immuno-Western blotting in epididymal tissue and fluid. The result showed that positive reactivity of phosphorylated proteins was clearly observed in cytoplasmic principle cells, nuclei of apical & basal cells and sperm mass surrounded with epididymal fluids. The profiles of phosphorylated proteins in epididymal fluid were 182, 127, 80, 70, 57, 45, 34, and 31 kDas, respectively. Interestingly, VPA affected the changes of phosphorylated proteins and β actin in head, body, and tail epididymal fluids. We conclude that tyrosine phosphorylated proteins were detected in epididymal epithelium and fluid. The expressions of those proteins and actin were altered under VPA treating.

Las proteínas tirosina fosforiladas han sido localizadas e identificadas en tejidos reproductores masculinos tales como testículos y espermatozoides, capacitados a nivel acrosómico, excepto en el epidídimo. Los cambios de estas proteínas están asociadas con una disminución de la calidad del esperma en el tratamiento con ácido valproico (AVP). Este estudio tuvo como objetivo investigar la presencia y las alteraciones de la fosforilación de proteínas en el epitelio epididimal y en el fluido espermático de ratas tratadas con AVP. Dieciséis ratas macho adultas se dividieron en dos grupos: control y tratadas con AVP (n = 8 / cada uno). A las ratas tratadas se les inyectó AVP por vía intraperitoneal (500 mg / kg de peso corporal) durante 10 días consecutivos. Al final del experimento, se realizó inmunohistoquímica con la anti-fosfotirosina monoclonal (clon 4G10) para sondear las proteínas tirosina fosforiladas y también para examinar la expresión de tales proteínas usando inmunotransferencia Western, en tejido y fluido epididimarios. El resultado mostró reactividad positiva de proteínas fosforiladas en células citoplásmicas principales, en los núcleos de las células apicales y basales y en la masa de esperma rodeada por fluidos epididimarios. Los perfiles de proteínas fosforiladas en el fluido epididimal fueron 182, 127, 80, 70, 57, 45, 34 y 31 kDas, respectivamente. El AVP provocó cambios en las proteínas fosforiladas y en la β actina de los fluidos epididimarios de cabeza, cuerpo y cola del epidídimo. Concluimos que las proteínas tirosina fosforiladas se detectaron en el epitelio y el fluido epididimarios. Las expresiones de esas proteínas y de la β actina se alteraron bajo tratamiento con AVP.
Descritores: Fosfoproteínas/efeitos dos fármacos
Tirosina/efeitos dos fármacos
Ácido Valproico/administração & dosagem
Actinas/efeitos dos fármacos
Anticonvulsivantes/administração & dosagem
-Fosfoproteínas/metabolismo
Fosforilação
Tirosina/metabolismo
Imuno-Histoquímica
Western Blotting
Actinas/metabolismo
Ratos Sprague-Dawley
Fosfotirosina
Epididimo
Limites: Animais
Masculino
Ratos
Responsável: CL1.1 - Biblioteca Central


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Id: biblio-893135
Autor: Chaichun, Amnart; Arun, Supatcharee; Burawat, Jaturon; Kanla, Pipatpong; Iamsaard, Sitthichai.
Título: Localization and identification of tyrosine phosphorylated proteins in adult Sprague-Dawley rat testis / Localización e identificación de proteínas de tirosina fosforilada en testículos de rata Sprague-Dawley adultos
Fonte: Int. j. morphol;35(4):1322-1327, Dec. 2017. graf.
Idioma: en.
Projeto: Khon Kaen University.
Resumo: SUMMARY: Spermatogenesis is a major process in testis occurring from puberty through life span of males. The tyrosine phosphorylation is assumed to play roles in spermatogenesis because this process is important for cell proliferations, divisions, and differentiations. However, the localizations and identifications of phosphorylated proteins in testicular tissue of adult male rats are still unclear. Therefore, this study attempted to immuno-localize and identify such proteins in testicular tissues of Sprague-Dawley rats. The monoclonal anti-phosphotyrosine (clone 4G10) was used to probe tyrosine phosphorylated proteins and also to examine the expression of such proteins using immuno-Western blotting in rat testis. The result showed that positive reactivity of tyrosine phosphorylated proteins was clearly observed in interstitial endocrine cells (Leydig cells), sustentocytes (Sertoli cells), spermatogonia, spermatocytes, and spermatids (round and elongated), respectively. The expressions of testicular tyrosine phosphorylated proteins were 200, 131, 93, 70, 60, and 48 kDas, respectively. In conclusion, testicular tyrosine phosphorylated proteins were localized in both germinal epithelium and interstitial endocrine cells of adult Sprague-Dawley rats.

RESUMEN: La espermatogénesis es un proceso importante en los testículos que ocurre desde la pubertad a lo largo de la vida de los machos. Se supone que la fosforilación de la tirosina desempeña papeles en la espermatogénesis, debido a que este proceso es importante para las proliferaciones, divisiones y diferenciaciones celulares. Sin embargo, las localizaciones e identificaciones de proteínas fosforiladas en el tejido testicular de ratas macho adultas todavía no están claras. Por lo tanto, este estudio intentó inmuno-localizar e identificar dichas proteínas en tejidos testiculares de ratas Sprague-Dawley. La anti-fosfotirosina monoclonal (clon 4G10) se usó para sondar proteínas tirosina fosforiladas y también para examinar la expresión de tales proteínas usando inmunotransferencia Western en testículo de rata. El resultado mostró que la actividad positiva de las proteínas tirosina fosforiladas se observó claramente en endocrinocitos intersticiales (células de Leydig), sustentocitos (células de Sertoli), espermatogonias, espermatocitos y espermátidas (redondas y alargadas), respectivamente. Las expresiones de las proteínas tirosina fosforiladas testiculares fueron de 200, 131, 93, 70, 60 y 48 kDas, respectivamente. En conclusión, las proteínas tirosina fosforiladas fueron localizadas en ambos epitelios germinales y endocrinocitos intersticiales de ratas adultas Sprague-Dawley.
Descritores: Fosfoproteínas/análise
Fosfoproteínas/metabolismo
Testículo/química
Tirosina/análise
Tirosina/metabolismo
-Imuno-Histoquímica
Western Blotting
Ratos Sprague-Dawley
Limites: Animais
Masculino
Ratos
Responsável: CL1.1 - Biblioteca Central


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Id: biblio-893038
Autor: Sampannang, Apichakan; Arun, Supatcharee; Sukhorum, Wannisa; Burawat, Jaturon; Nualkaew, Somsak; Maneenin, Chanwit; Sripanidkulchai, Bungorn; Iamsaard, Sitthichai.
Título: Antioxidant and hypoglycemic effects of Momordica cochinchinensis Spreng: (Gac) aril extract on reproductive damages in streptozotocin (STZ)-induced hyperglycemia mice / Efectos antioxidantes e hipoglucémicos del extracto de aril de Momordica cochinchinensis Spreng: (Gac) sobre los daños reproductivos en ratones con hiperglucemia inducida por streptozotocin (STZ)
Fonte: Int. j. morphol;35(2):667-675, June 2017. ilus.
Idioma: en.
Projeto: Khon Kaen University. Faculty of Medicine.
Resumo: The aim of present study was to investigate the effect of Momordica cochinchinensis (Gag) aril (GA) aqueous extract on male reproductive system of streptozotocin (STZ)-induced hyperglycemia (HG) mice. GA were extracted with distilled water (DW) and analyzed for in vitro antioxidant capacities. ICR male mice were divided into 7 groups: 1) control, 2) DW, 3) GA 1000 mg/kg BW, 4) HG, 5) HG + glibenclamide, 6 and 7) HG + GA 500 and 1000 mg/kg BW respectively (7 mice/ group). In HG groups, mice were induced by STZ at single dose (150 mg/kg BW). They were treated for consecutive 35 days. All groups were compared for blood glucose levels, weights and histopathologies of reproductive organs, sperm concentration including testicular tyrosine phosphorylation protein patterns by Immuno-Western blotting. The results showed that GA processed antioxidant activities and could significantly decrease blood glucose levels and increase sperm concentration in HG mice. Moreover, GA could change the density of a testicular 70 kDa protein in HG-GA groups. In conclusion, GA extract could improve hyperglycemia and male reproductive damages in STZ-induced HG mice.

El objetivo de este estudio fue investigar el efecto del extracto acuoso de Momordica cochinchinensis (Gag) aril (GA) en el sistema reproductor masculino de ratones hiperglucémicos inducidos por estreptozotocina (STZ). GA fue extraída con agua destilada (DW) y se analizaron las capacidades antioxidantes in vitro. Ratones ICR machos fueron divididos en 7 grupos: 1) control, 2) DW, 3) GA 1000 mg / kg PC, 4) HG, 5) HG + glibenclamida, 6 y 7) HG + GA 500 y 1000 mg / kg PC, respectivamente (7 ratones / grupo). En los grupos HG, los ratones fueron inducidos con STZ en dosis única (150 mg / kg BW). Fueron tratados durante 35 días consecutivos. En todos los grupos se compararon los niveles de glucosa en sangre, los pesos y las histopatologías de los órganos reproductores, la concentración de espermatozoides, incluídos los patrones testiculares de proteínas tirosina fosforilada por Inmuno-Western blot. Los resultados mostraron que GA procesaba actividades antioxidantes y podían disminuir significativamente los niveles de glucosa en sangre y aumentar la concentración de espermatozoides en ratones HG. Además, GA podría cambiar la densidad de una proteína testicular de 70 kDa en grupos HG-GA. En conclusión, el extracto de GA podría mejorar la hiperglucemia y los daños reproductivos masculinos inducidos por STZ en ratones HG.
Descritores: Doenças Testiculares/tratamento farmacológico
Extratos Vegetais/administração & dosagem
Momordica/química
Hiperglicemia/tratamento farmacológico
Hipoglicemiantes/administração & dosagem
Antioxidantes/administração & dosagem
-Fenóis/análise
Fosforilação/efeitos dos fármacos
Contagem de Espermatozoides
Espermatozoides/efeitos dos fármacos
Testículo/efeitos dos fármacos
Tirosina
Flavonoides/análise
Western Blotting
Diabetes Mellitus Experimental
Camundongos Endogâmicos ICR
Antioxidantes/química
Limites: Animais
Masculino
Camundongos
Responsável: CL1.1 - Biblioteca Central


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Id: biblio-888967
Autor: Manfredi, LH; Paula-Gomes, S; Zanon, NM; Kettelhut, IC.
Título: Myostatin promotes distinct responses on protein metabolism of skeletal and cardiac muscle fibers of rodents
Fonte: Braz. j. med. biol. res = Rev. bras. pesqui. méd. biol;50(12):e6733, 2017. graf.
Idioma: en.
Projeto: FAPESP; . CNPq.
Resumo: Myostatin is a novel negative regulator of skeletal muscle mass. Myostatin expression is also found in heart in a much less extent, but it can be upregulated in pathological conditions, such as heart failure. Myostatin may be involved in inhibiting protein synthesis and/or increasing protein degradation in skeletal and cardiac muscles. Herein, we used cell cultures and isolated muscles from rats to determine protein degradation and synthesis. Muscles incubated with myostatin exhibited an increase in proteolysis with an increase of Atrogin-1, MuRF1 and LC3 genes. Extensor digitorum longus muscles and C2C12 myotubes exhibited a reduction in protein turnover. Cardiomyocytes showed an increase in proteolysis by activating autophagy and the ubiquitin proteasome system, and a decrease in protein synthesis by decreasing P70S6K. The effect of myostatin on protein metabolism is related to fiber type composition, which may be associated to the extent of atrophy mediated effect of myostatin on muscle.
Descritores: Fibras Musculares Esqueléticas/efeitos dos fármacos
Fibras Musculares Esqueléticas/metabolismo
Miócitos Cardíacos/efeitos dos fármacos
Miócitos Cardíacos/metabolismo
Miostatina/farmacologia
Proteínas Musculares/efeitos dos fármacos
Proteínas Musculares/metabolismo
-Fosforilação/efeitos dos fármacos
Fosforilação/fisiologia
Fatores de Tempo
Tirosina/efeitos dos fármacos
Tirosina/metabolismo
Expressão Gênica
Células Cultivadas
Western Blotting
Reprodutibilidade dos Testes
Ratos Wistar
Reação em Cadeia da Polimerase em Tempo Real
Proteólise/efeitos dos fármacos
Limites: Animais
Masculino
Tipo de Publ: Estudo de Avaliação
Responsável: BR1.1 - BIREME



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