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Id: biblio-837660
Autor: Zhang, Wei; Liu, Kang; Li, Lei; Li, Yingxin; Sui, Xianxian; Rao, Yinzhu; Wu, Jiahao; Wu, Qiuping.
Título: Therapeutic effect of low molecular weight chitosan containing sepia ink on ethanol-induced gastric ulcer in rats
Fonte: Acta cir. bras;31(12):813-820, Dec. 2016. graf.
Idioma: en.
Projeto: Spark Program of State Ministry of Science and Technology; . Science and Technology Project of Zhanjiang.
Resumo: ABSTRACT PURPOSE: To evaluate the role of low molecular chitosan containing sepia ink (LMCS) in ethanol-induced (5 ml/kg) gastric ulcer in rats. METHODS: Animals were divided into four groups (n = 12): normal group (Normal), negative control group (Con), experiment group (LMCS) and positive control Omeprazole group (OMZ). Gastric empty rate was detected in the first 7 days. Rats were sacrificed at 7, 14 and 21 day for histology and ELISA detections. RESULTS: Gastric empty was no significant differences among the groups (P > 0.05). Histological observation showed gastric mucosal LMCS treated had better healing effect. Hydroxyproline (Hyp) was significantly increased from 7 day (P < 0.05). LMCS significantly inhibited malondialdehyde (MDA) generation for lipid peroxidation from 7 day (P < 0.05). LMCS significantly promoted the activity of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) at the earlier stage (P < 0.05). OMZ had the similar effects above. As for myeloperoxidase (MPO), LMCS significantly decreased and restored it to normal levels from 7 day (P < 0.05), it is earlier than OMZ which is from 14 day. CONCLUSION: LMCS can improve gastric mucosa tissue repair, exert significant influences on oxidative and antioxidant enzyme activities and neutrophil infiltration.
Descritores: Úlcera Gástrica/tratamento farmacológico
Quitosana/uso terapêutico
Sepia/química
Mucosa Gástrica/efeitos dos fármacos
Antiulcerosos/uso terapêutico
Antioxidantes/farmacologia
-Úlcera Gástrica/induzido quimicamente
Distribuição Aleatória
Quitosana/química
Modelos Animais de Doenças
Etanol
Mucosa Gástrica/patologia
Hidroxiprolina/metabolismo
Tinta
Malondialdeído/metabolismo
Peso Molecular
Antioxidantes/metabolismo
Limites: Animais
Ratos
Responsável: BR1.1 - BIREME


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Id: lil-783800
Autor: Girgin, Mustafa; Binnetoglu, Kenan; Duman, Kazim; Kanat, Burhan Hakan; Cetinkaya, Ziya; Ayten, Refik; Ilhan, Yavuz Selim; Ilhan, Necip; Seker, Ibrahim; Timurkaan, Necati.
Título: Effects of platelet rich plasma on fascial healing in rats with fecal peritonitis
Fonte: Acta cir. bras;31(5):314-319, May 2016. tab, graf.
Idioma: en.
Resumo: ABSTRACT PURPOSE : To evaluate the effects of platelet rich plasma (PRP) on the healing of fascia wherein peritonitis has been created. METHODS: Twenty eight Wistar Albino rats were divided into four groups. Only a primary fascial repair following laparotomy was performed on Group 1, a primary fascial repair performed and PRP treatment applied following laparotomy on Group 2, and a fecal peritonitis created following laparotomy and a primary fascial repair carried out on Group 3. A fecal peritonitis was created following laparotomy and primary fascial repair and PRP treatment on the fascia was carried out on Group 4. RESULTS: TNF-α was found to be significantly lower in the control group (Group 1). It was detected at the highest level in the group in which fecal peritonitis was created and PRP applied (Group 4). TGF-β was determined as being significantly higher only in Group 4. Histopathologically, the differences between the groups in terms of cell infiltration and collagen deposition were not found to be significant. CONCLUSION: When platelet rich plasma was given histologically and biochemicaly as wound healing parameters cellular infiltration, collagen accumulation, and tissue hydroxyiproline levels were not increased but neovascularization, fibroblast activation and TNF Alfa levels were increased and PRP accelerated wound healing.
Descritores: Peritonite/complicações
Cicatrização
Plasma Rico em Plaquetas
Fáscia/fisiologia
-Peritonite/metabolismo
Serina Endopeptidases/metabolismo
Distribuição Aleatória
Fator de Crescimento Transformador beta/análise
Fator de Crescimento Transformador beta/metabolismo
Colágeno/efeitos dos fármacos
Colágeno/metabolismo
Fator de Necrose Tumoral alfa/análise
Fator de Necrose Tumoral alfa/metabolismo
Ratos Wistar
Gelatinases/metabolismo
Neovascularização Fisiológica
Modelos Animais
Fáscia/irrigação sanguínea
Hidroxiprolina/análise
Hidroxiprolina/metabolismo
Proteínas de Membrana/metabolismo
Limites: Animais
Responsável: BR1.1 - BIREME


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Id: lil-775562
Autor: Zhang, Wei; Wang, Yan; Sui, Xianxian; Sun, Yulin; Chen, Daohai.
Título: Effects of chitin and sepia ink hybrid sponge on the healing of burning wound rats and its impact on macrophages in vitro
Fonte: Acta cir. bras;31(2):119-125, Feb. 2016. graf.
Idioma: en.
Projeto: Spark Program of State Ministry of Science & Technology.
Resumo: PURPOSE: To detect whether chitin and sepia ink sponge (CS) can promote wound healing and elevate impact of CS on phagocytosis ability of macrophages. METHODS: Forty-eight rats were assigned to four groups: Normal group (Normal), negative control group (Con), chitin and sepia ink sponge group (CS) and positive control Surgicel Gauze(r) group (SG). Deep second-degree burn model was created in rats. Wound area was recorded by digital imaging and determined using Image J software. Samples were collected and kept at -80oC on 3d, 7d, 14d and 21d for cytokines detecting. Transforming growth factor (TGF)-β1, interleukin (IL)-6, matrix metalloproteinase (MMP)-1, hydroxyproline (Hyp) and macrophage activity reflected by tumor necrosis factor (TNF)-α were determined by enzyme-linked immunosorbent assay (ELISA). RESULTS: Comparing to Con and SG, scabs in CS group fell off and basically healed on 21 day. TGF-β1, IL-6, MMP-1 and Hyp were significantly increased by CS and SG comparing to Con (p < 0.05), CS had more apparently adjustment on TGF-β1 and MMP-1 compared to SG; results in vitro indicated CS significantly promoted phagocytosis ability of macrophages reflected in TNF-α (p < 0.05). CONCLUSION: CS improved wound healing through exerting significant influences on secretion of kinds of cytokines and activating macrophages.
Descritores: Cicatrização/efeitos dos fármacos
Queimaduras Químicas/tratamento farmacológico
Quitina/farmacologia
Sepia
Macrófagos/efeitos dos fármacos
-Fagocitose/efeitos dos fármacos
Distribuição Aleatória
Quitina/uso terapêutico
Citocinas/efeitos dos fármacos
Citocinas/metabolismo
Ratos Wistar
Metaloproteinase 1 da Matriz/efeitos dos fármacos
Modelos Animais de Doenças
Hidroxiprolina/metabolismo
Tinta
Macrófagos/metabolismo
Limites: Animais
Masculino
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


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Id: biblio-888960
Autor: Chen, XJ; Liu, S; Gao, GZ; Yan, DX; Jiang, WS.
Título: Effects of vacuum sealing drainage on the treatment of cranial bone-exposed wounds in rabbits
Fonte: Braz. j. med. biol. res = Rev. bras. pesqui. méd. biol;50(12):e5837, 2017. graf.
Idioma: en.
Projeto: Army Medical and Health Care Research Fund.
Resumo: This study was designed to assess the efficacy of vacuum sealing drainage (VSD) on skull exposure wounds in rabbits and to investigate the underlying mechanism of the process. Full-thickness excisional circular wounds 2×2 cm with or without periosteum involvement were created in 88 New Zealand white rabbits (mean body weight: 3.0±0.65 kg). Animals were randomly divided into 4 groups: periosteum-intact wounds treated with traditional dressing (p+control), periosteum-intact wounds treated with VSD (p+VSD), periosteum-lacking wounds treated with traditional dressing (p-control) and periosteum-lacking wounds treated with VSD (p-VSD). The wounds treated with traditional dressing were covered with Vaseline gauze, while VSD treatment was accompanied with continuous -120 mmHg pressure. Finally, wound tissues were harvested for analysis of hydroxyproline content and histologic detection. VSD hastened the wound healing process significantly (P<0.05) compared to the corresponding control groups. VSD alleviated the inflammation reaction, accelerated re-epithelialization and facilitated the organization of collagen fibers into neat rows. During the wound healing process, the hydroxyproline content increased overtime [i.e., postoperative days (POD) 7, POD 10 and POD 15] in all four groups, and it peaked in the p+VSD group. VSD also promoted angiogenesis via increasing number and quality of collagen. We concluded that VSD can promote healing in bone-exposed wounds via increasing hydroxyproline content and vessel density, reducing inflammatory responses and generating ordered collagen arrangement.
Descritores: Crânio/lesões
Bandagens
Drenagem/métodos
Tratamento de Ferimentos com Pressão Negativa/métodos
-Crânio/patologia
Neovascularização Fisiológica
Modelos Animais de Doenças
Microvasos
Hidroxiprolina/análise
Limites: Animais
Coelhos
Responsável: BR1.1 - BIREME


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Id: biblio-984633
Autor: Tumay, L. Volkan; Kilicturgay, Sadik; Savci, Vahide; Saraydaroglu, Ozlem; Anarat, Ruksan.
Título: Effects of Aloe Vera on healing of colonic anastomoses: experimental rat study / Efeitos da Aloe Vera na cicatrização de anastomoses colônicas: estudo experimental em ratos
Fonte: J. coloproctol. (Rio J., Impr.);39(1):33-40, Jan.-Mar. 2019. tab.
Idioma: en.
Resumo: ABSTRACT Background: Although herbal medicinal products are being used widely throughout the World, beneficial and harmful effects have not been well documented. Our aim was to evaluate the effects of Aloe Vera (AV) on colonic anastomosis healing. Material and methods: 112 albino Wistar rats were randomly assigned into five main groups: preoperative Aloe Vera Group (P), pre- and postoperative Aloe Vera Group (PP), Control Group (C), sham Aloe Vera Group (SA) and Sham Control Group (SC). Groups P, PP, and SA received 1.6 mL/kg per day Aloe Vera by orogastric feeding catheter for 1 month prior to the experiment. Groups P, PP, and C underwent anastomosis of the distal colon, and subgroups (n = 4) of each were sacrificed on postoperative day 3, 7, 14 and 21. Anastomotic bursting pressure, perianastomotic collagen content and histopathological changes were studied. Results: The SC Group had significantly higher ABP when compared with the SA Group (p = 0.0002), although hydroxyproline content showed no difference. When ABP was compared between anastomosis groups, it was found significantly lower in Aloe Vera groups on Day 3 (P3 vs. C3, p = 0.003 and PP3 vs. C3, p = 0.007). Hydroxyproline content was significantly lower in Group PP than Group C, also on Day 3 (p = 0.05). Significant difference was not detected after Day 3 in any of the study parameters. Conclusion: Aloe Vera decreased tissue collagen content in the early postoperative period. It is advisable to call into question the concomitant usage of conventional medicine and the herbal supplements for the surgeons in their clinical practice.

RESUMO Fundamentação: Embora os medicamentos à base de plantas sejam amplamente utilizados no mundo inteiro, seus efeitos (benéficos e prejudiciais) não estão bem documentados. Este estudo teve como objetivo avaliar os efeitos da Aloe vera (AV) na cicatrização de anastomoses colônicas. Material e métodos: 112 ratos Wistar albinos foram distribuídos aleatoriamente em cinco grupos principais: AV pré-operatório (P), AV pré e pós-operatório (PP), controle (C), sham AV (SA) e sham controle (SC). Os grupos P, PP e SA receberam AV em uma dose de 1,6 mL/kg por dia por sonda de alimentação orogástrica por 1 mês antes do experimento. Os grupos P, PP e C foram submetidos a anastomose do cólon distal. Subgrupos (n = 4) de cada grupo foram sacrificados no terceiro, sétimo, 14° e 21° dias pós-operatórios. Os seguintes parâmetros foram avaliados: pressão de ruptura anastomótica (PRA), conteúdo de colágeno perianastomótico e alterações histopatológicas. Resultados: O grupo SC apresentou PRA significativamente maior quando comparado ao grupo SA (p = 0,0002), embora o conteúdo de hidroxiprolina não tenha apresentado diferença. Ao comparar a PRA entre os grupos de anastomose, ela foi significativamente menor no terceiro dia nos grupos que usaram AV (P3 vs. C3, p = 0,003 e PP3 vs. C3, p = 0,007). No terceiro dia, o teor de hidroxiprolina foi significativamente menor no grupo PP do que no grupo C (p = 0,05). Após o terceiro dia, não se observou diferença significativa em nenhum dos parâmetros do estudo. Conclusão: O uso de AV diminuiu o conteúdo de colágeno tecidual no período pós-operatório imediato. É aconselhável questionar o uso concomitante da medicina convencional e suplementos fitoterápicos na prática clínica.
Descritores: Anastomose Cirúrgica/reabilitação
Aloe
-Cicatrização
Ratos Wistar
Colo/patologia
Medicamentos Fitoterápicos
Hidroxiprolina
Limites: Animais
Ratos
Responsável: BR545.3 - Biblioteca ICBS


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Id: lil-748222
Autor: Zhang, L.; Ji, Y.X.; Jiang, W.L.; Lv, C.J..
Título: Protective roles of pulmonary rehabilitation mixture in experimental pulmonary fibrosis in vitro and in vivo
Fonte: Braz. j. med. biol. res = Rev. bras. pesqui. méd. biol;48(6):545-552, 06/2015. tab, graf.
Idioma: en.
Projeto: National Natural Science Foundation of China; . Taishan Scholar Project to Fang Han.
Resumo: Abnormal high mobility group protein B1 (HMGB1) activation is involved in the pathogenesis of pulmonary fibrosis. Pulmonary rehabilitation mixture (PRM), which combines extracts from eight traditional Chinese medicines, has very good lung protection in clinical use. However, it is not known if PRM has anti-fibrotic activity. In this study, we investigated the effects of PRM on transforming growth factor-β1 (TGF-β1)-mediated and bleomycin (BLM)-induced pulmonary fibrosis in vitro and in vivo. The effects of PRM on TGF-β1-mediated epithelial-mesenchymal transition (EMT) in A549 cells, on the proliferation of human lung fibroblasts (HLF-1) in vitro, and on BLM-induced pulmonary fibrosis in vivo were investigated. PRM treatment resulted in a reduction of EMT in A549 cells that was associated with attenuating an increase of vimentin and a decrease of E-cadherin. PRM inhibited the proliferation of HLF-1 at an IC50 of 0.51 µg/mL. PRM ameliorated BLM-induced pulmonary fibrosis in rats, with reduction of histopathological scores and collagen deposition, and a decrease in α-smooth muscle actin (α-SMA) and HMGB1 expression. An increase in receptor for advanced glycation end-product (RAGE) expression was found in BLM-instilled lungs. PRM significantly decreased EMT and prevented pulmonary fibrosis through decreasing HMGB1 and regulating RAGE in vitro and in vivo. PRM inhibited TGF-β1-induced EMT via decreased HMGB1 and vimentin and increased RAGE and E-cadherin levels. In summary, PRM prevented experimental pulmonary fibrosis by modulating the HMGB1/RAGE pathway.
Descritores: Medicamentos de Ervas Chinesas/farmacologia
Fibrose Pulmonar/tratamento farmacológico
Fibrose Pulmonar/prevenção & controle
-Antibióticos Antineoplásicos
Receptor para Produtos Finais de Glicação Avançada/efeitos dos fármacos
Apoptose/efeitos dos fármacos
Bleomicina
Western Blotting
Células Cultivadas
Colágeno/efeitos dos fármacos
Misturas Complexas/farmacologia
Medicamentos de Ervas Chinesas/uso terapêutico
Transição Epitelial-Mesenquimal/efeitos dos fármacos
Fibroblastos/efeitos dos fármacos
Proteína HMGB1/efeitos dos fármacos
Hidroxiprolina/análise
Imuno-Histoquímica
Pulmão/efeitos dos fármacos
Pulmão/patologia
Fator de Crescimento Derivado de Plaquetas/efeitos dos fármacos
Fibrose Pulmonar/patologia
Distribuição Aleatória
Ratos Sprague-Dawley
Reprodutibilidade dos Testes
Fator de Crescimento Transformador beta1/efeitos dos fármacos
Limites: Animais
Seres Humanos
Masculino
Tipo de Publ: Estudos de Avaliação
Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


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Id: lil-712970
Autor: Parra, E.R.; Pincelli, M.S.; Teodoro, W.R.; Velosa, A.P.P.; Martins, V.; Rangel, M.P.; Barbas-Filho, J.V.; Capelozzi, V.L..
Título: Modeling pulmonary fibrosis by abnormal expression of telomerase/apoptosis/collagen V in experimental usual interstitial pneumonia
Fonte: Braz. j. med. biol. res = Rev. bras. pesqui. méd. biol;47(7):567-575, 07/2014. tab, graf.
Idioma: en.
Projeto: FAPESP.
Resumo: Limitations on tissue proliferation capacity determined by telomerase/apoptosis balance have been implicated in pathogenesis of idiopathic pulmonary fibrosis. In addition, collagen V shows promise as an inductor of apoptosis. We evaluated the quantitative relationship between the telomerase/apoptosis index, collagen V synthesis, and epithelial/fibroblast replication in mice exposed to butylated hydroxytoluene (BHT) at high oxygen concentration. Two groups of mice were analyzed: 20 mice received BHT, and 10 control mice received corn oil. Telomerase expression, apoptosis, collagen I, III, and V fibers, and hydroxyproline were evaluated by immunohistochemistry, in situ detection of apoptosis, electron microscopy, immunofluorescence, and histomorphometry. Electron microscopy confirmed the presence of increased alveolar epithelial cells type 1 (AEC1) in apoptosis. Immunostaining showed increased nuclear expression of telomerase in AEC type 2 (AEC2) between normal and chronic scarring areas of usual interstitial pneumonia (UIP). Control lungs and normal areas from UIP lungs showed weak green birefringence of type I and III collagens in the alveolar wall and type V collagen in the basement membrane of alveolar capillaries. The increase in collagen V was greater than collagens I and III in scarring areas of UIP. A significant direct association was found between collagen V and AEC2 apoptosis. We concluded that telomerase, collagen V fiber density, and apoptosis evaluation in experimental UIP offers the potential to control reepithelization of alveolar septa and fibroblast proliferation. Strategies aimed at preventing high rates of collagen V synthesis, or local responses to high rates of cell apoptosis, may have a significant impact in pulmonary fibrosis.
Descritores: Apoptose/fisiologia
Colágeno Tipo V/biossíntese
Fibrose Pulmonar Idiopática/patologia
Fibrose Pulmonar/patologia
Telomerase/metabolismo
-Hidroxitolueno Butilado
Proliferação Celular
Colágeno Tipo I/análise
Colágeno Tipo II/análise
Colágeno Tipo V/análise
Modelos Animais de Doenças
Células Epiteliais/metabolismo
Células Epiteliais/patologia
Imunofluorescência
Fibroblastos/metabolismo
Fibroblastos/patologia
Hidroxiprolina/análise
Imuno-Histoquímica
Marcação In Situ das Extremidades Cortadas
Camundongos Endogâmicos BALB C
Microscopia Eletrônica
Alvéolos Pulmonares/patologia
Alvéolos Pulmonares/ultraestrutura
Coloração e Rotulagem
Telomerase/isolamento & purificação
Limites: Animais
Masculino
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


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Id: lil-705782
Autor: Buen, Eliseo Portilla-de; Orozco-Mosqueda, Abel; Leal-Cortés, Caridad; Vázquez-Camacho, Gonzalo; Fuentes-Orozco, Clotilde; Alvarez-Villaseñor, Andrea Socorro; Macías-Amezcua, Michel Dassaejv; González-Ojeda, Alejandro.
Título: Fibrinogen and thrombin concentrations are critical for fibrin glue adherence in rat high-risk colon anastomoses
Fonte: Clinics;69(4):259-264, 4/2014. tab, graf.
Idioma: en.
Projeto: SIMORELOS; . FOFOI.
Resumo: OBJECTIVE: Fibrin glues have not been consistently successful in preventing the dehiscence of high-risk colonic anastomoses. Fibrinogen and thrombin concentrations in glues determine their ability to function as sealants, healers, and/or adhesives. The objective of the current study was to compare the effects of different concentrations of fibrinogen and thrombin on bursting pressure, leaks, dehiscence, and morphology of high-risk ischemic colonic anastomoses using fibrin glue in rats. METHODS: Colonic anastomoses in adult female Sprague-Dawley rats (weight, 250-350 g) treated with fibrin glue containing different concentrations of fibrinogen and thrombin were evaluated at post-operative day 5. The interventions were low-risk (normal) or high-risk (ischemic) end-to-end colonic anastomoses using polypropylene sutures and topical application of fibrinogen at high (120 mg/mL) or low (40 mg/mL) concentrations and thrombin at high (1000 IU/mL) or low (500 IU/mL) concentrations. RESULTS: Ischemia alone, anastomosis alone, or both together reduced the bursting pressure. Glues containing a low fibrinogen concentration improved this parameter in all cases. High thrombin in combination with low fibrinogen also improved adherence exclusively in low-risk anastomoses. No differences were detected with respect to macroscopic parameters, histopathology, or hydroxyproline content at 5 days post-anastomosis. CONCLUSIONS: Fibrin glue with a low fibrinogen content normalizes the bursting pressure of high-risk ischemic left-colon anastomoses in rats at day 5 after surgery. .
Descritores: Colo/cirurgia
Adesivo Tecidual de Fibrina/uso terapêutico
Fibrinogênio/administração & dosagem
Isquemia/prevenção & controle
Trombina/administração & dosagem
Adesivos Teciduais/uso terapêutico
-Anastomose Cirúrgica
Colágeno/análise
Colo/irrigação sanguínea
Colo/patologia
Hidroxiprolina/análise
Isquemia/etiologia
Pressão
Ratos Sprague-Dawley
Reprodutibilidade dos Testes
Fatores de Risco
Resultado do Tratamento
Cicatrização
Limites: Animais
Feminino
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


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Id: lil-689977
Autor: Minicucci, Marcos F.; Santos, Priscila P. dos; Rafacho, Bruna P.M.; Goncalves, Andrea F.; Ardisson, Lidiane P.; Batista, Diego F.; Azevedo, Paula S.; Polegato, Bertha F.; Okoshi, Katashi; Pereira, Elenize J.; Paiva, Sergio A.R.; Zornoff, Leonardo A.M..
Título: Periostin as a modulator of chronic cardiac remodeling after myocardial infarction
Fonte: Clinics;68(10):1344-1349, out. 2013. tab.
Idioma: en.
Resumo: OBJECTIVE: After acute myocardial infarction, during the cardiac repair phase, periostin is released into the infarct and activates signaling pathways that are essential for the reparative process. However, the role of periostin in chronic cardiac remodeling after myocardial infarction remains to be elucidated. Therefore, the objective of this study was to investigate the relationship between tissue periostin and cardiac variables in the chronic cardiac remodeling induced by myocardial infarction. METHODS: Male Wistar rats were assigned to 2 groups: a simulated surgery group (SHAM; n = 8) and a myocardial infarction group (myocardial infarction; n = 13). After 3 months, morphological, functional and biochemical analyses were performed. The data are expressed as means±SD or medians (including the lower and upper quartiles). RESULTS: Myocardial infarctions induced increased left ventricular diastolic and systolic areas associated with a decreased fractional area change and a posterior wall shortening velocity. With regard to the extracellular matrix variables, the myocardial infarction group presented with higher values of periostin and types I and III collagen and higher interstitial collagen volume fractions and myocardial hydroxyproline concentrations. In addition, periostin was positively correlated with type III collagen levels (r = 0.673, p = 0.029) and diastolic (r = 0.678, p = 0.036) and systolic (r = 0.795, p = 0.006) left ventricular areas. Considering the relationship between periostin and the cardiac function variables, periostin was inversely correlated with both the fractional area change (r = -0.783, p = 0.008) and the posterior wall shortening velocity (r = -0.767, p = 0.012). CONCLUSIONS: Periostin might be a modulator of deleterious cardiac remodeling in the chronic phase after myocardial infarction in rats. .
Descritores: Moléculas de Adesão Celular/metabolismo
Infarto do Miocárdio/metabolismo
Remodelação Ventricular/fisiologia
-Western Blotting
Colágeno Tipo I/análise
Colágeno Tipo III/análise
Modelos Animais de Doenças
Diástole/fisiologia
Hidroxiprolina/análise
Infarto do Miocárdio/fisiopatologia
Infarto do Miocárdio
Ratos Wistar
Sístole/fisiologia
Disfunção Ventricular Esquerda/fisiopatologia
Disfunção Ventricular Esquerda
Função Ventricular Esquerda/fisiologia
Limites: Animais
Masculino
Ratos
Responsável: BR1.1 - BIREME


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Id: lil-644221
Autor: Monteiro, JC; Gomes, MLM; Nakagaki, WR; Tomiosso, TC; Sbervelheri, MM; Dolder, H.
Título: Does heteropterys aphrodisiaca administration andendurance training alter bones of mature rats?
Fonte: Braz. j. morphol. sci = Rev. bras. ciênc. morfol;27(2):67-73, Apr.-June 2010. ilus, tab, graf.
Idioma: en.
Resumo: Heteropterys aphrodisiaca infusion, alone or associated with endurance training, was investigated in rat bonesin relation to their mechanical properties, collagen content and morphology. Male rats were divided into fourgroups (n = 8): CS- control sedentary, HS- H. aphrodisiaca sedentary, CT–control trained, HT‑H. aphrodisiacatrained. The training protocol consisted in running on a motorized treadmill, 5 times a week, for 8 weeks,with weekly increase in treadmill velocity and duration. Control groups received water while HS and HTgroups received H. aphrodisiaca infusion (104 mg/animal) by gavage during the 8 weeks. Tibiae werefrozen for collagen dosage and biomechanical analysis or preserved in Karnovsky's fixative, then processedfor histomorphological analysis by conventional light microscopy and scanning electron microscopy. TheHT group showed significantly higher yield load and yield stress in the tibiae three-point bending test. Themaximum load, stiffness, maximum stress and elastic modulus were statistically similar for the experimentalgroups. The hydroxyproline content, morphometrical and stereological data were not significantly differentfor the four groups. Scanning electron microscopy showed more lacunae and Havers canals in the bone oftrained animals, moreover the osteons were more disorganized, when compared with sedentary groups. Thesealterations may indicate that the bone of trained animals was being remodeled. However, after 8 weeks oftraining, it was not possible verify alterations in morphometrical measurements, collagen content, stiffness andmodulus of elasticity of the trained and treated animals.
Descritores: Osso e Ossos
Hidroxiprolina
Tíbia
Tíbia/anatomia & histologia
-Fenômenos Biomecânicos
Microscopia Eletrônica
Ratos Wistar
Limites: Animais
Adulto
Ratos
Responsável: BR734.1 - Biblioteca Central Cesar Lattes - BCCL



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