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Id: biblio-866649
Autor: Modena, Karin Cristina da Silva.
Título: Influência de materiais odontológicos na capacidade de resposta de fibroblastos cultivados de polpa dental humana / Influence of dental materials on the response capability of cultured fibroblasts from human dental pulp.
Fonte: Bauru; s.n; 2012. 138 p. ilus, tab, graf.
Idioma: pt.
Tese: Apresentada a Universidade de São Paulo. Faculdade de Odontologia de Bauru para obtenção do grau de Doutor.
Resumo: O presente trabalho tem como objetivo investigar a influência de materiais utilizados na prática odontológica (Single Bond, HEMA, Vitrebond, Ketac Molar e Dycal) na resposta inflamatória de fibroblastos cultivados de polpa dental humana de dentes permanentes em relação à expressão e produção de mediadores da inflamação. As culturas primárias de fibroblastos foram estabelecidas a partir do tecido pulpar de terceiros molares hígidos. Após a quarta passagem, os fibroblastos foram estimulados pelos materiais e pelos materiais seguidos por LPS de E. coli pelos tempos de 6 e 24 horas. Os testes utilizados foram: MTT, Trypan Blue, Análise de Griess, PCR quantitativo e ELISA. Os dados foram analisados estatisticamente aplicando-se o teste ANOVA a 1 critério e pós-teste de Tukey e ANOVA a 2 critérios e teste de correção de Bonferroni (p<0,05). Os materiais SB10 (Single Bond 1:100) e DY (Dycal) afetaram a viabilidade celular com diminuição do metabolismo. Os materiais SB1 (Single Bond 1:1.000), SB10 (Single Bond 1:100) e VB (Vitrebond) seguidos de LPS de E. coli diminuíram o metabolismo celular de maneira estatisticamente significativa. Os níveis de óxido nítrico produzidos foram diminuídos quando os fibroblastos foram estimulados pelo KM (Ketac Molar). A expressão gênica para pró-colágeno tipo I foi diminuída quando os fibroblastos foram estimulados pelos materiais SB10 (Single Bond 1:100), SB (Single Bond polimerizado) e DY (Dycal). Para o SDF-1_/CXCL12 houve um aumento da expressão para o grupo estimulado apenas por LPS de E. coli, SB10 (Single Bond 1:100) e DY (Dycal). Para o IL-6 notou-se uma diminuição significativa para o grupo estimulado por H1000 (HEMA 1000 nM) e um aumento para o grupo SB10 (Single Bond 1:100). A expressão gênica de IL- 8/CXCL8 diminuiu para os fibroblastos estimulados pelas três concentrações de HEMA e de Single Bond, VB (Vitrebond) e DY (Dycal) no período de 6 horas e houve um aumento para os materiais SB10 (Single Bond 1:100) e VB...

The aim of the present study is to investigate the influence of dental materials (Single Bond, HEMA, Vitrebond, Ketac Molar e Dycal) in the inflammatory response of human dental pulp fibroblasts from permanent teeth in relation to inflammatory mediators expression. and production. Primary cultures were established from third molars pulp tissue. After the fourth passage, the fibroblasts were stimulated only by materials and also by the materials followed by LPS from E. coli for 6 and 24 hours. Data were statistically analyzed using Oneway ANOVA and Tukey post-test and Two-way ANOVA followed by Bonferroni post-test (p<0.05). SB10 (Single Bond 1: 100) and DY (Dycal) affected cell viability and consequently decreased cell metabolism. SB1 (Single Bond 1:1,000), SB10 (Single Bond 1:100) and VB (Vitrebond) followed by LPS E. coli decreased cell metabolism. Nitric oxide levels were reduced when fibroblasts were stimulated by KM (Ketac Molar). Pro-collagen type I expression was reduced when fibroblasts were stimulated by SB10 (Single Bond 1:100), SB (polymerized Single Bond) and DY (Dycal). SDF-1_/CXCL12 expression was increased for the group stimulated only by LPS from E. coli, SB10 (Single Bond 1:100) and DY (Dycal). IL-6 expression had a significant decrease in the group stimulated by H1000 (HEMA 1000 nM) and an increase for SB10 (Single Bond 1:100) group. The expression of IL-8/CXCL8 decreased when fibroblasts were stimulated by the three concentrations of HEMA and of Single Bond, VB (Vitrebond) and DY (Dycal) at 6 hours and increased for SB10 (Single Bond 1:100) and VB (Vitrebond) at 24 hours. There was decrease in SDF-1_/CXCL12 production for the three concentrations of HEMA and DY (Dycal) and a declining trend for the other materials tested. The production of IL-6 was increased by VB (Vitrebond) and KM (Ketac Molar). The production of IL-8/CXCL8 increased by SB1 (Single Bond 1:1,000), VB...
Descritores: Fibroblastos
Materiais Dentários/toxicidade
Polpa Dentária
-Análise de Variância
Colágeno Tipo I/análise
Ensaio de Imunoadsorção Enzimática
/análise
INTERLEUCINA-ABDOMEN, ACUTE/análise
Dente Molar
Metaloproteinases da Matriz/análise
Metaloproteinases da Matriz
Reação em Cadeia da Polimerase
Quimiocinas CXC/análise
Quimiocinas CXC
Fatores de Tempo
Limites: Humanos
Responsável: BR28.1 - Serviço de Biblioteca e Documentação Professor Doutor Antônio Gabriel Atta


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Rasslan, Samir
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Id: lil-771855
Autor: Pinheiro, Daniel Faria de Campos; Fontes, Belchor; Shimazaki, John Kioshi; Heimbecker, Ana Maria Cattani; Jacysyn, Jacqueline de Fátima; Rasslan, Samir; Montero, Edna Frasson de Souza; Utiyama, Edivaldo Massazo.
Título: Ischemic preconditioning modifies mortality and inflammatory response
Fonte: Acta cir. bras;31(1):1-7, Jan. 2016. graf.
Idioma: en.
Resumo: PURPOSE: To evaluate the effect of ischemic preconditioning on mortality, inflammatory mediators and oxidative stress after intestinal ischemia and reperfusion. METHODS: Male Wistar rats were allocated according to the period of ischemia with or without ischemic preconditioning which consist on clamping the superior mesenteric artery for 10 minutes followed by reperfusion for 10 minutes before the sustained ischemia period. Mortality was assessed in Phase 1 study, and the CINC-1, CINC-2 and MDA levels in the lungs were analyzed in Phase 2. RESULTS: Mortality was lower in the ischemic preconditioning group subjected to 90 minutes of ischemia compared to the group without ischemic preconditioning (I-90: 50% and IPC-90: 15%, p=0.018), and it was lower in the ischemic preconditioning group as a whole compared to the groups without ischemic preconditioning (IPC-14% and I=30%, p=0.006). Lower levels of MDA, CINC-1, and CINC-2 were observed in the animals that were subjected to ischemic preconditioning compared to the animals that were not (MDA: I-45=1.23 nmol/mg protein, and IPC-45=0.62 nmol/mg protein, p=0.0333; CINC-1: I-45=0.82 ng/mL and IPC-45=0.67 ng/mL, p=0.041; CINC-2: I-45=0.52 ng/mL and IPC-45=0.35 ng/mL, p=0.032). CONCLUSION: Ischemic preconditioning reduces mortality, inflammatory process and oxidative stress in rats subjected to intestinal ischemia and reperfusion.
Descritores: Mediadores da Inflamação/metabolismo
Precondicionamento Isquêmico/mortalidade
Isquemia Mesentérica/metabolismo
Estresse Oxidativo/imunologia
Traumatismo por Reperfusão/mortalidade
-Quimiocina CXCL1/análise
Quimiocinas CXC/análise
Ensaio de Imunoadsorção Enzimática
Pulmão/metabolismo
Pulmão/fisiopatologia
Malondialdeído/análise
Artérias Mesentéricas/metabolismo
Isquemia Mesentérica/mortalidade
Ratos Wistar
Estatísticas não Paramétricas
Limites: Animais
Masculino
Responsável: BR1.1 - BIREME


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Id: lil-707678
Autor: Modena, Karin Cristina da Silva.
Título: Influência de materiais odontológicos na capacidade de resposta de fibroblastos cultivados de polpa dental humana / Influence of dental materials on the response capability of cultured fibroblasts from human dental pulp.
Fonte: Bauru; s.n; 2012. 138 p. ilus, tab, graf.
Idioma: pt.
Tese: Apresentada a Universidade de São Paulo. Faculdade de Odontologia de Bauru para obtenção do grau de Doutor.
Resumo: O presente trabalho tem como objetivo investigar a influência de materiais utilizados na prática odontológica (Single Bond, HEMA, Vitrebond, Ketac Molar e Dycal) na resposta inflamatória de fibroblastos cultivados de polpa dental humana de dentes permanentes em relação à expressão e produção de mediadores da inflamação. As culturas primárias de fibroblastos foram estabelecidas a partir do tecido pulpar de terceiros molares hígidos. Após a quarta passagem, os fibroblastos foram estimulados pelos materiais e pelos materiais seguidos por LPS de E. coli pelos tempos de 6 e 24 horas. Os testes utilizados foram: MTT, Trypan Blue, Análise de Griess, PCR quantitativo e ELISA. Os dados foram analisados estatisticamente aplicando-se o teste ANOVA a 1 critério e pós-teste de Tukey e ANOVA a 2 critérios e teste de correção de Bonferroni (p<0,05). Os materiais SB10 (Single Bond 1:100) e DY (Dycal) afetaram a viabilidade celular com diminuição do metabolismo. Os materiais SB1 (Single Bond 1:1.000), SB10 (Single Bond 1:100) e VB (Vitrebond) seguidos de LPS de E. coli diminuíram o metabolismo celular de maneira estatisticamente significativa. Os níveis de óxido nítrico produzidos foram diminuídos quando os fibroblastos foram estimulados pelo KM (Ketac Molar). A expressão gênica para pró-colágeno tipo I foi diminuída quando os fibroblastos foram estimulados pelos materiais SB10 (Single Bond 1:100), SB (Single Bond polimerizado) e DY (Dycal). Para o SDF-1_/CXCL12 houve um aumento da expressão para o grupo estimulado apenas por LPS de E. coli, SB10 (Single Bond 1:100) e DY (Dycal). Para o IL-6 notou-se uma diminuição significativa para o grupo estimulado por H1000 (HEMA 1000 nM) e um aumento para o grupo SB10 (Single Bond 1:100). A expressão gênica de IL- 8/CXCL8 diminuiu para os fibroblastos estimulados pelas três concentrações de HEMA e de Single Bond, VB (Vitrebond) e DY (Dycal) no período de 6 horas e houve um aumento para os materiais SB10 (Single Bond 1:100) e VB...

The aim of the present study is to investigate the influence of dental materials (Single Bond, HEMA, Vitrebond, Ketac Molar e Dycal) in the inflammatory response of human dental pulp fibroblasts from permanent teeth in relation to inflammatory mediators expression. and production. Primary cultures were established from third molars pulp tissue. After the fourth passage, the fibroblasts were stimulated only by materials and also by the materials followed by LPS from E. coli for 6 and 24 hours. Data were statistically analyzed using Oneway ANOVA and Tukey post-test and Two-way ANOVA followed by Bonferroni post-test (p<0.05). SB10 (Single Bond 1: 100) and DY (Dycal) affected cell viability and consequently decreased cell metabolism. SB1 (Single Bond 1:1,000), SB10 (Single Bond 1:100) and VB (Vitrebond) followed by LPS E. coli decreased cell metabolism. Nitric oxide levels were reduced when fibroblasts were stimulated by KM (Ketac Molar). Pro-collagen type I expression was reduced when fibroblasts were stimulated by SB10 (Single Bond 1:100), SB (polymerized Single Bond) and DY (Dycal). SDF-1_/CXCL12 expression was increased for the group stimulated only by LPS from E. coli, SB10 (Single Bond 1:100) and DY (Dycal). IL-6 expression had a significant decrease in the group stimulated by H1000 (HEMA 1000 nM) and an increase for SB10 (Single Bond 1:100) group. The expression of IL-8/CXCL8 decreased when fibroblasts were stimulated by the three concentrations of HEMA and of Single Bond, VB (Vitrebond) and DY (Dycal) at 6 hours and increased for SB10 (Single Bond 1:100) and VB (Vitrebond) at 24 hours. There was decrease in SDF-1_/CXCL12 production for the three concentrations of HEMA and DY (Dycal) and a declining trend for the other materials tested. The production of IL-6 was increased by VB (Vitrebond) and KM (Ketac Molar). The production of IL-8/CXCL8 increased by SB1 (Single Bond 1:1,000), VB...
Descritores: Fibroblastos
Materiais Dentários/toxicidade
Polpa Dentária
-Análise de Variância
Colágeno Tipo I/análise
Ensaio de Imunoadsorção Enzimática
/análise
INTERLEUCINA-ABDOMEN, ACUTE/análise
Dente Molar
Metaloproteinases da Matriz/análise
Metaloproteinases da Matriz
Reação em Cadeia da Polimerase
Quimiocinas CXC/análise
Quimiocinas CXC
Fatores de Tempo
Limites: Humanos
Responsável: BR28.1 - Serviço de Biblioteca e Documentação Professor Doutor Antônio Gabriel Atta


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Texto completo SciELO Chile
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Id: lil-582863
Autor: Aguayo, Francisco; Murayama, Tsugiya; Eizuru, Yoshito.
Título: UL146 variability among clinical isolates of Human Cytomegalovirus from Japan
Fonte: Biol. Res;43(4):475-480, 2010. ilus, tab.
Idioma: en.
Resumo: Human Cytomegalovirus (HCMV) is a herpesvirus associated with serious diseases in immunocompromised subjects. The region between ORF UL133 and UL151 from HCMV, named ULb' is frequently deleted in attenuated AD169 and in highly passaged laboratory strains. However, this region is conserved in low-passaged and more virulent HCMV, like the Toledo strain. The UL146 gene, which is located in the ULb' region, encodes a CXC-chemokine analogue. The diversity of UL146 gene was evaluated among fifty-six clinical isolates of HCMV from Japan. Results show that UL146 gene was successfully amplified by the polymerase chain reaction (PCR) in only 17/56 strains (30 percent), while the success rate for UL145/UL147 gene was 18/56 strains (32 percent). After DNA sequencing, the 35 amplified strains were classified into 8 groups. When compared, variability of UL146 ranged from 25.1 percent to 52.9 percent at the DNA level and from 34.5 percent to 67 percent at the amino acid level. Seven groups had the interleukin-8 (IL-8) motif ERL (Glu-Leu-Arg) CXC and one group had only the CXC motif, suggesting the absence of the IL-8 function of UL146. In conclusion, we found that UL146 gene of HCMV is hyper-variable in clinical strains from Japan suggesting the possibility of a different function in each sequence group.
Descritores: Quimiocinas CXC/genética
Infecções por Citomegalovirus/virologia
Citomegalovirus/genética
Genes Virais/genética
Variação Genética/genética
Proteínas Virais/genética
-Sequência de Bases
Citomegalovirus/isolamento & purificação
Fibroblastos/virologia
Genótipo
Japão
Dados de Sequência Molecular
Reação em Cadeia da Polimerase
Análise de Sequência de DNA
Limites: Humanos
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


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Texto completo
Id: lil-534173
Autor: Almeida, Caroline de Souza; Abramo, Clarice; Alves, Caio César de Souza; Mazzoccoli, Luciano; Ferreira, Ana Paula; Teixeira, Henrique Couto.
Título: Anti-mycobacterial treatment reduces high plasma levels of CXC-chemokines detected in active tuberculosis by cytometric bead array
Fonte: Mem. Inst. Oswaldo Cruz;104(7):1039-1041, Nov. 2009. ilus.
Idioma: en.
Projeto: CNPq; . FAPEMIG; . CBB PPM; . WOTRO; . CAPES.
Resumo: Chemokines recruit and activate leukocytes, assisting granuloma formation. Herein, we evaluated plasma chemokines in patients with active tuberculosis (ATB) and after completing treatment (TTB) and compared them to BCG-vaccinated healthy controls (HC). Levels of chemokines were measured by cytometric bead array. Levels of CXCL8, CXCL9 and CXCL10 were higher in ATB patients compared to HC, but they decreased in TTB. Levels of CCL2 and CCL5 in ATB patients were similar to those observed in HC. Thus, the high levels of CXC-chemokines detected during ATB, which can modulate the trafficking of immune cells from the periphery to the site of infection, were reversed by anti-mycobacterial treatment.
Descritores: Antibióticos Antituberculose/uso terapêutico
Quimiocinas CXC/sangue
Tuberculose Pulmonar/sangue
Tuberculose Pulmonar/tratamento farmacológico
-Vacina BCG
Estudos de Casos e Controles
Quimiocinas CXC/análise
Citometria de Fluxo/métodos
Estatísticas não Paramétricas
Fatores de Tempo
Resultado do Tratamento
Adulto Jovem
Limites: Adolescente
Adulto
Idoso
Feminino
Humanos
Masculino
Pessoa de Meia-Idade
Adulto Jovem
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


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Id: lil-425080
Autor: Moreira, M. A; Souza, A. L. S; Lana-Peixoto, M. A; Teixeira, M. M; Teixeira, A. L.
Título: Chemokines in the cerebrospinal fluid of patients with active and stable relapsing-remitting multiple sclerosis
Fonte: Braz. j. med. biol. res = Rev. bras. pesqui. méd. biol;39(4):441-445, Apr. 2006. ilus.
Idioma: en.
Resumo: Multiple sclerosis (MS) is a chronic inflammatory demyelinating disease of the human central nervous system. Although its etiology is unknown, the accumulation and activation of mononuclear cells in the central nervous system are crucial to its pathogenesis. Chemokines have been proposed to play a major role in the recruitment and activation of leukocytes in inflammatory sites. They are divided into subfamilies on the basis of the location of conserved cysteine residues. We determined the levels of some CC and CXC chemokines in the cerebrospinal fluid (CSF) of 23 relapsing-remitting MS patients under interferon-ß-1a therapy and 16 control subjects using ELISA. MS patients were categorized as having active or stable disease. CXCL10 was significantly increased in the CSF of active MS patients (mean ± SEM, 369.5 ± 69.3 pg/mL) when compared with controls (178.5 ± 29.1 pg/mL, P < 0.05). CSF levels of CCL2 were significantly lower in active MS (144.7 ± 14.4 pg/mL) than in controls (237.1 ± 16.4 pg/mL, P < 0.01). There was no difference in the concentration of CCL2 and CXCL10 between patients with stable MS and controls. CCL5 was not detectable in the CSF of most patients or controls. The qualitative and quantitative differences of chemokines in CSF during relapses of MS suggest that they may be useful as a marker of disease activity and of the mechanisms involved in the pathogenesis of the disease.
Descritores: Quimiocinas CC/líquido cefalorraquidiano
Quimiocinas CXC/líquido cefalorraquidiano
Esclerose Múltipla Recidivante-Remitente/líquido cefalorraquidiano
-Adjuvantes Imunológicos/uso terapêutico
Biomarcadores/líquido cefalorraquidiano
Estudos de Casos e Controles
Ensaio de Imunoadsorção Enzimática
Interferon beta/uso terapêutico
Esclerose Múltipla Recidivante-Remitente/tratamento farmacológico
Limites: Adulto
Feminino
Humanos
Masculino
Pessoa de Meia-Idade
Responsável: BR1.1 - BIREME


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Id: lil-409271
Autor: Neiva, K; Sun, Y. X; Taichman, R. S.
Título: The role of osteoblasts in regulating hematopoietic stem cell activity and tumor metastasis
Fonte: Braz. j. med. biol. res = Rev. bras. pesqui. méd. biol;38(10):1449-1454, Oct. 2005.
Idioma: en.
Conferência: Apresentado em: SIMEC 2004 (International Symposium on Extracellular Matrix), Angra dos Reis, September 27-30, 2004.
Projeto: Tissue Procurement Core of the University of Michigan Comprehensive Cancer Center; . University of Michigan Core Centers for Musculoskeletal Disorders; . National Institutes of Health.
Resumo: Bone marrow stromal cells are critical regulators of hematopoiesis. Osteoblasts are part of the stromal cell support system in bone marrow and may be derived from a common precursor. Several studies suggested that osteoblasts regulate hematopoiesis, yet the entire mechanism is not understood. It is clear, however, that both hematopoietic precursors and osteoblasts interact for the production of osteoclasts and the activation of resorption. We observed that hematopoietic stem cells (HSCs) regulate osteoblastic secretion of various growth factors, and that osteoblasts express some soluble factors exclusively in the presence of HSCs. Osteoblasts and hematopoietic cells are closely associated with each other in the bone marrow, suggesting a reciprocal relationship between them to develop the HSC niche. One critical component regulating the niche is stromal-derived factor-1 (SDF-1) and its receptor CXCR4 which regulates stem cell homing and, as we have recently demonstrated, plays a crucial role in facilitating those tumors which metastasize to bone. Osteoblasts produce abundant amounts of SDF-1 and therefore osteoblasts play an important role in metastasis. These findings are discussed in the context of the role of osteoblasts in marrow function in health and disease.
Descritores: Neoplasias Ósseas/secundário
Quimiocinas CXC/metabolismo
Células-Tronco Hematopoéticas/fisiologia
Osteoblastos/fisiologia
/metabolismo
RECEPTORS, CXCRABBREVIATIONS AS TOPIC/metabolismo
-Neoplasias Ósseas/metabolismo
CHEMOKINE CXCLABETALIPOPROTEINEMIA
Osteoblastos/citologia
Osteoblastos/metabolismo
Células Estromais/metabolismo
Limites: Animais
Humanos
Tipo de Publ: Revisão
Responsável: BR1.1 - BIREME



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