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Id: biblio-1098314
Autor: Kalmukova, Olesia; Yurchenko, Alona; Savchuk, Olexii; Dzerzhynsky, Mykola.
Título: Variable beige adipocyte morphology in obese rats by different times of melatonin administration / Variación de la morfología del adipocito beige en ratas obesas según los diferentes tiempos de administración de melatonina
Fonte: Int. j. morphol;38(3):737-746, June 2020. tab, graf.
Idioma: en.
Resumo: This study aimed to evaluate changes in beige adipocytes at different times of melatonin administration, in the morning (ZT01) or in the evening (ZT11), at 30 mg/kg daily by gavage for 7 weeks or continuously with drinking water in the term of high-calorie diet-induced obesity (HCD). Melatonin received at ZT11 or with drinking water resulted in an increased area of the browning zone in the subcutaneous white adipose tissue (sWAT), even in rats with HCD (compared with Control or HCD, respectively). The beige adipocyte and lipid droplet area after melatonin use were reduced compared to those with HCD and Control, in all administration modes (group ZT01 showed smaller changes compared to ZT11 or with drinking water groups). The fibrosis level decreased and significantly differed in HCD ZT01, HCD ZT11, and HCD water compared to that in HCD; moreover, the lowest value determined in HCD water, reached the control parameters. Furthermore, the IL-1b and IL-8 level was decreased in the HCD groups under melatonin treatment at ZT11 or with drinking water compared to that in HCD. The obtained results suggest that melatonin promotes sWAT browning in rats with diet-induced obesity and influences morphological signs of normal rats depending on the time of administration. Different functional activity of beige adipocytes was observed after melatonin was used depending on the time of administration, resulting in heat production and lipolysis (the relative mass of visceral fat was likewise diminished). More rapid browning was observed when melatonin treatment was performed at 1 h before lights-off (ZT11) or continuously via drinking water. Melatonin acted on beige adipocytes of obese rats through changing some parameters such as the area of adipocytes and lipid drops, the number of lipid drops, the relative area browning of sWAT, and the level of tissue fibrosis.

Este estudio tuvo como objetivo evaluar los cambios en los adipocitos beige en diferentes momentos de la administración de melatonina, en la mañana (ZT01) o por la noche (ZT11). Se administraron 30 mg/kg diariamente por sonda durante 7 semanas o continuamente con agua potable durante el periodo de obesidad inducida por una dieta alta en calorías (HCD). La melatonina recibida en ZT11 o con agua potable resultó en un aumento de área dorada en tejido adiposo blanco subcutáneo (sWAT), incluso en ratas con HCD (en comparación con Control o HCD, respectivamente). El área de gotas de lípidos y adipocitos de color beige después del uso de melatonina se redujo en comparación con aquellos con HCD y Control, en todos los modos de administración (el grupo ZT01 mostró cambios más pequeños en comparación con ZT11 o con grupos de agua potable). El nivel de fibrosis disminuyó y difirió significativamente en HCD ZT01, HCD ZT11 y agua HCD, en comparación con el HCD; además, el valor más bajo determinado en agua HCD alcanzó los parámetros de control. Además, el nivel de IL-1b e IL-8 disminuyó en los grupos HCD bajo tratamiento con melatonina en ZT11 o con agua potable en comparación con el de HCD. Los resultados obtenidos sugieren que la melatonina promueve el dorado sWAT en ratas con obesidad inducida por la dieta e influye en los signos morfológicos de las ratas normales dependiendo del momento de la administración. Se observó una actividad funcional diferente de los adipocitos de color beige después de usar melatonina dependiendo del tiempo de administración, dando como resultado la producción de calor y lipólisis (la masa relativa de grasa visceral también disminuyó). Se observó un ennegrecimiento más rápido cuando el tratamiento con melatonina se realizó 1 h antes de apagar las luces (ZT11) o de forma continua en grupos de agua potable. La melatonina actuó en los adipocitos beige de ratas obesas al cambiar algunos parámetros, como el área de adipocitos y gotas de lípidos, el número de gotas de lípidos, el área relativa de ennegrecimiento de sWAT y el nivel de fibrosis tisular.
Descritores: Adipócitos Bege/efeitos dos fármacos
Melatonina/administração & dosagem
Obesidade
-Fatores de Tempo
Fibrose
Tecido Adiposo/efeitos dos fármacos
Tecido Adiposo/patologia
Interleucina-8/efeitos dos fármacos
Dieta
Interleucina-1beta/efeitos dos fármacos
Limites: Animais
Masculino
Ratos
Responsável: CL1.1 - Biblioteca Central


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Souza, Dorotéia Rossi Silva
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Id: biblio-894075
Autor: Furlan, Larissa Lazzarini; Ribeiro, José Dirceu; Bertuzzo, Carmen Sílvia; Salomão Junior, João Batista; Souza, Dorotéia Rossi Silva; Marson, Fernando Augusto Lima.
Título: Variants in the interleukin 8 gene and the response to inhaled bronchodilators in cystic fibrosis / Variantes no gene da interleucina 8 e a resposta a broncodilatadores inalatórios na fibrose cística
Fonte: J. pediatr. (Rio J.);93(6):639-648, Nov.-Dec. 2017. tab, graf.
Idioma: en.
Projeto: Fundação de Amparo à Pesquisa do Estado de São Paulo; . Extensão da Universidade Estadual de Campinas; . FAPESP.
Resumo: Abstract Objective: Interleukin 8 protein promotes inflammatory responses, even in airways. The presence of interleukin 8 gene variants causes altered inflammatory responses and possibly varied responses to inhaled bronchodilators. Thus, this study analyzed the interleukin 8 variants (rs4073, rs2227306, and rs2227307) and their association with the response to inhaled bronchodilators in cystic fibrosis patients. Methods: Analysis of interleukin 8 gene variants was performed by restriction fragment length polymorphism of polymerase chain reaction. The association between spirometry markers and the response to inhaled bronchodilators was evaluated by Mann-Whitney and Kruskal-Wallis tests. The analysis included all cystic fibrosis patients, and subsequently patients with two mutations in the cystic fibrosis transmembrane conductance regulator gene belonging to classes I to III. Results: This study included 186 cystic fibrosis patients. There was no association of the rs2227307 variant with the response to inhaled bronchodilators. The rs2227306 variant was associated with FEF50% in the dominant group and in the group with two identified mutations in the cystic fibrosis transmembrane conductance regulator gene. The rs4073 variant was associated with spirometry markers in four genetic models: co-dominant (FEF25-75% and FEF75%), dominant (FEV1, FEF50%, FEF75%, and FEF25-75%), recessive (FEF75% and FEF25-75%), and over-dominant (FEV1/FVC). Conclusions: This study highlighted the importance of the rs4073 variant of the interleukin 8 gene, regarding response to inhaled bronchodilators, and of the assessment of mutations in the cystic fibrosis transmembrane conductance regulator gene.

Resumo Objetivo: A proteína interleucina 8 promove respostas inflamatórias, o que inclui sua atuação nas vias aéreas. A presença de variantes no gene da interleucina 8 causa respostas inflamatórias alteradas e possivelmente respostas variadas ao uso de broncodilatadores inalatórios. Assim, este estudo analisou as variantes da interleucina 8 (rs4073, rs2227306, rs2227307) e sua associação à resposta a broncodilatadores inalatórios em pacientes com fibrose cística. Métodos: Foi feita análise das variantes genéticas da interleucina 8 por restriction fragment length polymorphism da reação em cadeia da polimerase. A associação entre os marcadores da espirometria e a resposta a broncodilatadores inalatórios foi feita pelos testes de Mann-Whitney e Kruskal-Wallis. A análise incluiu todos os pacientes com fibrose cística e posteriormente pacientes com duas mutações no gene cystic fibrosis transmembrane conductance regulator pertencentes às Classes I a II. Resultados: Este estudo incluiu 186 pacientes com fibrose cística. Não houve associação da variante rs2227307 à resposta a broncodilatadores inalatórios. A variante rs2227306 foi associada a FEF50% no grupo dominante e no grupo com duas mutações identificadas no gene cystic fibrosis transmembrane conductance regulator. A variante rs4073 foi associada a marcadores da espirometria em quatro modelos genéticos: codominante (FEF25-75% e FEF75%), dominante (VEF1, FEF50%, FEF75% e FEF25-75%), recessivo (FEF75% e FEF25-75%) e overdominante (VEF1/CVF). Conclusões: Este estudo destaca, principalmente, a importância da variante rs4073 do gene da interleucina 8, na resposta a broncodilatadores inalatórios, concomitantemente ao genótipo das mutações no gene cystic fibrosis transmembrane conductance regulator.
Descritores: Broncodilatadores/uso terapêutico
Interleucina-8/efeitos dos fármacos
Regulador de Condutância Transmembrana em Fibrose Cística/genética
Fibrose Cística/genética
Fibrose Cística/tratamento farmacológico
-Espirometria
Índice de Gravidade de Doença
Polimorfismo de Fragmento de Restrição
Reação em Cadeia da Polimerase
Estudos Transversais
Interleucina-8/genética
Genótipo
Mutação
Limites: Humanos
Masculino
Feminino
Criança
Adolescente
Adulto
Pessoa de Meia-Idade
Idoso
Adulto Jovem
Responsável: BR1.1 - BIREME


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Xavier, Ricardo Machado
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Id: lil-722291
Autor: Salim, Patricia Hartstein; Xavier, Ricardo Machado.
Título: Influência dos polimorfismos genéticos (IL10/CXCL8/CXCR2/ NF?B) na susceptibilidade das doenças reumatológicas autoimunes / Influence of genetic polymorphisms (IL-10/CXCL8/CXCR2/ NF?B) on the susceptibility of autoimmune rheumatic diseases
Fonte: Rev. bras. reumatol;54(4):301-310, Jul-Aug/2014. tab.
Idioma: pt.
Resumo: As doenças reumatológicas autoimunes, na maioria das vezes, possuem uma via genética comum para a autoimunidade. Vários genes foram associados com as doenças reumatológicas, para tanto iremos analisar somente alguns genes nos quais há várias evidências da existência de associação com risco ou proteção de doença autoimune. O fator de transcrição nuclear kappa B (NF-kappa B), o qual regula as respostas imunes e inflamatórias, está associado com esclerose sistêmica (ES), artrite reumatoide (AR) e lúpus eritematoso sistêmico (LES), assim como os genes CXCR2 e CXCL8. Já a interleucina 10 (IL-10), que é uma citocina anti-inflamatória, está associada com quase todas as doenças reumatológicas. Neste artigo, revisamos os potenciais papéis desses genes no sistema imunológico e em diversas doenças reumatológicas. Com relação à IL-10, diversos estudos foram realizados, porém em sua maioria contraditórios - alguns encontraram ausência de associação e outros encontraram associação em diferentes polimorfismos do genes. Já em relação ao NF-kappa B, somente foi estudado em AR e LES, e não foram observadas análises significativas relevantes. Os polimorfismos genéticos do gene CXCR2 foram associados com ES, mas não estão associados com AR e LES. Já os polimorfismos genéticos do gene CXCL8 não estão associados com ES, mas estão associados com AR.

The autoimmune rheumatologic disorders mostly have a common genetic path to the autoimmunity. Several genes have been associated with rheumatologic disorders; therefore, we are analyzing just the ones in those containing several evidences of the existence of association with the risk or protection from autoimmune disorder. The nuclear factor kappa beta (NF-kappa B), which regulates the autoimmune and anti-inflammatory responses, is associated with systemic sclerosis (SS), rheumatoid arthritis (RA) and systemic lupus erythematosus (SLE), just as the CXCR2 e CXCL8 genes. On the other hand, the interleukin-10 (IL-10), which is an anti-inflammatory cytokine, is associated with almost all rheumatologic disorders. In this article, we are reviewing the potential roles of these genes in the immune system and in several rheumatologic disorders. In relation to IL-10, several studies have been carried out, but most of them are controversial - some detected the absence of association, and others found association in different genetic polymorphisms. Conversely, in relation to NF-kappa B, it was studied just in RA and SLE, and no relevant significant analyses were observed. The genetic polymorphisms of the CXCR2 gene were associated with SS, but not with RA e SLE. On the other side, the genetic polymorphisms of the CXCL8 gene are not associated with SS, but with RA.
Descritores: Polimorfismo Genético
Doenças Autoimunes/genética
Doenças Reumáticas/genética
Predisposição Genética para Doença
-Doenças Reumáticas/imunologia
Interleucina-8/genética
NF-kappa B/genética
Interleucina-10/genética
Receptores de Interleucina-8B/genética
Limites: Humanos
Tipo de Publ: Revisão
Responsável: BR1.1 - BIREME


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Id: biblio-1054698
Autor: Xia, Wenfang; Li, Guang; Pan, Zhou; Zhou, Qingshan.
Título: Hypercapnia attenuates ventilator-induced lung injury through vagus nerve activation
Fonte: Acta cir. bras;34(9):e201900902, 2019. tab, graf.
Idioma: en.
Projeto: National Natural Science Foundation of China; . Hubei Natural Science Foundation of Youth.
Resumo: Abstract Purpose: To investigate the role of vagus nerve activation in the protective effects of hypercapnia in ventilator-induced lung injury (VILI) rats. Methods: Male Sprague-Dawley rats were randomized to either high-tidal volume or low-tidal volume ventilation (control) and monitored for 4h. The high-tidal volume group was further divided into either a vagotomy or sham-operated group and each surgery group was further divided into two subgroups: normocapnia and hypercapnia. Injuries were assessed hourly through hemodynamics, respiratory mechanics and gas exchange. Protein concentration, cell count and cytokines (TNF-α and IL-8) in bronchoalveolar lavage fluid (BALF), lung wet-to-dry weight and pathological changes were examined. Vagus nerve activity was recorded for 1h. Results: Compared to the control group, injurious ventilation resulted in a decrease in PaO2/FiO2 and greater lung static compliance, MPO activity, enhanced BALF cytokines, protein concentration, cell count, and histology injury score. Conversely, hypercapnia significantly improved VILI by decreasing the above injury parameters. However, vagotomy abolished the protective effect of hypercapnia on VILI. In addition, hypercapnia enhanced efferent vagus nerve activity compared to normocapnia. Conclusion: These results indicate that the vagus nerve plays an important role in mediating the anti-inflammatory effect of hypercapnia on VILI.
Descritores: Nervo Vago/cirurgia
Líquido da Lavagem Broncoalveolar/química
Lesão Pulmonar Induzida por Ventilação Mecânica/prevenção & controle
Hipercapnia
-Vagotomia
Distribuição Aleatória
Citocinas/análise
Interleucina-8/análise
Fator de Necrose Tumoral alfa/análise
Ratos Sprague-Dawley
Modelos Animais de Doenças
Limites: Animais
Masculino
Ratos
Responsável: BR1.1 - BIREME


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Id: biblio-949354
Autor: Turhan, Ali Haydar; Atıcı, Aytuğ; Sürmeli, Serra.
Título: Effects of hypothermia on lung inflammation in a rat model of meconium aspiration syndrome
Fonte: Acta cir. bras;33(6):483-490, June 2018. tab, graf.
Idioma: en.
Resumo: Abstract Purpose: To evaluate the effects of hypothermia treatment on meconium-induced inflammation. Methods: Fifteen rats were instilled with human meconium (MEC, 1.5 mL/kg, 65 mg/mL) intratracheally and ventilated for 3 hours. Eight rats that were ventilated and not instilled with meconium served as a sham group. In MEC-hypothermia group, the body temperature was lowered to 33±0.5°C. Analysis of the blood gases, interleukin (IL)-1β, IL-6, IL-8, and tumor necrosis factor (TNF)-α in bronchoalveolar lavage (BAL) fluid samples, and histological analyses of the lungs were performed. Results: The BAL fluid TNF-α, IL-1β, IL-6 and IL-8 concentrations were significantly higher in the MEC-hypothermia group than in the MEC-normothermia (p < 0.001, p < 0.001, p = 0.001, p < 0.001, respectively) and sham-controlled groups (p < 0.001, p < 0.001, p < 0.001, p < 0.001, respectively). Conclusion: Meconium-induced inflammatory cytokine production is affected by the body temperature control.
Descritores: Pneumonia/patologia
Síndrome de Aspiração de Mecônio/patologia
Síndrome de Aspiração de Mecônio/terapia
Hipotermia Induzida/métodos
-Pneumonia/metabolismo
Pneumonia/terapia
Ensaio de Imunoadsorção Enzimática
Líquido da Lavagem Broncoalveolar/química
Síndrome de Aspiração de Mecônio/metabolismo
Reprodutibilidade dos Testes
Interleucina-8/metabolismo
Interleucina-6/metabolismo
Fator de Necrose Tumoral alfa/metabolismo
Resultado do Tratamento
Ratos Wistar
Modelos Animais de Doenças
Interleucina-1beta/metabolismo
Medições Luminescentes/métodos
Pulmão/patologia
Limites: Animais
Masculino
Tipo de Publ: Estudo de Avaliação
Responsável: BR1.1 - BIREME


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Id: lil-761962
Autor: Palomino, Diana Carolina Torres; Marti, Luciana Cavalheiro.
Título: Chemokines and immunity / Quimiocinas e imunidade
Fonte: Einstein (Säo Paulo);13(3):469-473, July-Sep. 2015. tab.
Idioma: en.
Projeto: FAPESP.
Resumo: Chemokines are a large family of small cytokines and generally have low molecular weight ranging from 7 to 15kDa. Chemokines and their receptors are able to control the migration and residence of all immune cells. Some chemokines are considered pro-inflammatory, and their release can be induced during an immune response at a site of infection, while others are considered homeostatic and are involved in controlling of cells migration during tissue development or maintenance. The physiologic importance of this family of mediators is resulting from their specificity − members of the chemokine family induce recruitment of well-defined leukocyte subsets. There are two major chemokine sub-families based upon cysteine residues position: CXC and CC. As a general rule, members of the CXC chemokines are chemotactic for neutrophils, and CC chemokines are chemotactic for monocytes and sub-set of lymphocytes, although there are some exceptions. This review discusses the potential role of chemokines in inflammation focusing on the two best-characterized chemokines: monocyte chemoattractant protein-1, a CC chemokine, and interleukin-8, a member of the CXC chemokine sub-family.

Quimiocinas são uma grande família de pequenas citocinas e seu peso molecular varia de 7 a 15kDa. As quimiocinas e seus receptores são capazes de controlar a migração e a residência de células imunes. Algumas quimiocinas são consideradas pró-inflamatórias e podem ser induzidas durante a resposta imune no sítio de infecção, enquanto outras são consideradas homeostáticas e estão envolvidas no controle da migração celular durante o desenvolvimento ou a manutenção dos tecidos. A importância fisiológica dessa família de mediadores é resultado de sua especificidade − os membros da família de quimiocinas induzem ao recrutamento de subtipos bem definidos de leucócitos. Existem duas grandes subfamílias de quimiocinas baseadas na posição dos resíduos de cisteínas: CXC e CC. Como regra geral, membros da família de quimiocinas CXC são quimiotáticos de neutrófilos, e as quimiocinas CC são quimiotáticos de monócitos e subtipos de linfócitos, apesar de existirem algumas exceções. Esta revisão discute o potencial papel das quimiocinas na inflamação focando nas duas quimiocinas mais bem caracterizadas: a proteína quimioatraente de monócitos-1, uma quimiocina CC, e a interleucina 8, uma quimiocina membro da subfamília CXC.
Descritores: Quimiocinas/imunologia
-Fragmentos de Peptídeos/imunologia
Doença Aguda
Interleucina-8/imunologia
Quimiocina CCL2/imunologia
Inflamação/imunologia
Limites: Humanos
Responsável: BR1.1 - BIREME


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Id: lil-787548
Autor: KNOB, Carollinie Dias; SILVA, Milena; GASPAROTO, Thaís Helena; OLIVEIRA, Carine Ervolino; AMÔR, Nádia Ghinelli; ARAKAWA, Nilton Syogo; COSTA, Fernando Batista; CAMPANELLI, Ana Paula.
Título: Effects of budlein A on human neutrophils and lymphocytes
Fonte: J. appl. oral sci;24(3):271-277graf.
Idioma: en.
Projeto: São Paulo Research Foundation; . São Paulo Research Foundation; . São Paulo Research Foundation.
Resumo: ABSTRACT Sesquiterpene lactones (SLs) are the active constituents of a variety of medicinal plants used in traditional medicine for the treatment of inflammatory diseases and other ailments. Objective In this study, we evaluated whether budlein A modulates the activation of innate and adaptive immune cells such as neutrophils and lymphocytes. Material and Methods Our research group has investigated several plant species and several compounds have been isolated, identified, and their medical potential evaluated. Budlein A is a SL isolated from the species Aldama buddlejiformis and A. robusta (Asteraceae) and shows anti-inflammatory and anti-nociceptive activities. Advances in understanding how plant-derived substances modulate the activation of innate and adaptive immune cells have led to the development of new therapies for human diseases. Results Budlein A inhibited MPO activity, IL-6, CXCL8, IL-10, and IL-12 production and induces neutrophil apoptosis. In contrast, budlein A inhibited lymphocyte proliferation and IL-2, IL-10, TGF-β, and IFN-γ production, but it did not lead to cell death. Conclusions Collectively, our results indicate that budlein A shows distinct immunomodulatory effects on immune cells.
Descritores: Sesquiterpenos/farmacologia
Linfócitos T/efeitos dos fármacos
Lactonas/farmacologia
Anti-Inflamatórios/farmacologia
Neutrófilos/efeitos dos fármacos
-Ensaio de Imunoadsorção Enzimática
Fatores de Crescimento Transformadores/análise
Fatores de Crescimento Transformadores/efeitos dos fármacos
Células Cultivadas
Reprodutibilidade dos Testes
Análise de Variância
Interleucina-8/análise
Interleucina-8/efeitos dos fármacos
Interleucinas/análise
Apoptose/efeitos dos fármacos
Peroxidase/análise
Peroxidase/efeitos dos fármacos
Asteraceae/química
Proliferação de Células/efeitos dos fármacos
Citometria de Fluxo
Limites: Humanos
Masculino
Feminino
Adulto
Pessoa de Meia-Idade
Tipo de Publ: Estudo de Avaliação
Responsável: BR1.1 - BIREME


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Id: lil-777353
Autor: Mendi, Ayşegül; Köse, Sevil; Uçkan, Duygu; Akca, Gülçin; Yilmaz, Derviş; Aral, Levent; Gültekin, Sibel Elif; Eroğlu, Tamer; Kiliç, Emine; Uçkan, Sina.
Título: Lactobacillus rhamnosus could inhibit Porphyromonas gingivalis derived CXCL8 attenuation
Fonte: J. appl. oral sci;24(1):67-75, Jan.-Feb. 2016. tab, graf.
Idioma: en.
Projeto: TUBITAK; . TUBITAK; . TUBITAK.
Resumo: ABSTRACT An increasing body of evidence suggests that the use of probiotic bacteria is a promising intervention approach for the treatment of inflammatory diseases with a polymicrobial etiology. P. gingivalis has been noted to have a different way of interacting with the innate immune response of the host compared to other pathogenic bacteria, which is a recognized feature that inhibits CXCL8 expression. Objective The aim of the study was to determine if P. gingivalis infection modulates the inflammatory response of gingival stromal stem cells (G-MSSCs), including the release of CXCL8, and the expression of TLRs and if immunomodulatory L. rhamnosus ATCC9595 could prevent CXCL8 inhibition in experimental inflammation. Material and Methods G-MSSCs were pretreated with L. rhamnosus ATCC9595 and then stimulated with P. gingivalis ATCC33277. CXCL8 and IL-10 levels were investigated with ELISA and the TLR-4 and 2 were determined through flow cytometer analysis. Results CXCL8 was suppressed by P. gingivalis and L. rhamnosus ATCC9595, whereas incubation with both strains did not abolish CXCL8. L. rhamnosus ATCC9595 scaled down the expression of TLR4 and induced TLR2 expression when exposed to P. gingivalis stimulation (p<0.01). Conclusions These findings provide evidence that L. rhamnosus ATCC9595 can modulate the inflammatory signals and could introduce P. gingivalis to immune systems by inducing CXCL8 secretion.
Descritores: Interleucina-8/análise
Porphyromonas gingivalis/imunologia
Probióticos/farmacologia
Lactobacillus rhamnosus/fisiologia
Células-Tronco Mesenquimais/microbiologia
-Periodontite/microbiologia
Aderência Bacteriana/imunologia
Ensaio de Imunoadsorção Enzimática
Células Cultivadas
Interleucina-8/imunologia
Interferon gama/análise
Interferon gama/imunologia
Interleucina-10
Estatísticas não Paramétricas
Receptor 4 Toll-Like/análise
Receptor 4 Toll-Like/imunologia
Citometria de Fluxo
Imunidade Inata
Limites: Humanos
Adulto Jovem
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


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Id: lil-787423
Autor: Utiyama, Daniela Mitiyo Odagiri; Yoshida, Carolina Tieko; Goto, Danielle Miyuki; de Santana Carvalho, Tômas; de Paula Santos, Ubiratan; Koczulla, Andreas Rembert; Saldiva, Paulo Hilário Nascimento; Nakagawa, Naomi Kondo.
Título: The effects of smoking and smoking cessation on nasal mucociliary clearance, mucus properties and inflammation
Fonte: Clinics;71(6):344-350tab, graf.
Idioma: en.
Projeto: Fundação de Amparo è Pesquisa do Estado de São Paulo.
Resumo: OBJECTIVE: The aim of the present study was to assess nasal mucociliary clearance, mucus properties and inflammation in smokers and subjects enrolled in a Smoking Cessation Program (referred to as quitters). METHOD: A total of 33 subjects with a median (IQR) smoking history of 34 (20-58) pack years were examined for nasal mucociliary clearance using a saccharine transit test, mucus properties using contact angle and sneeze clearability tests, and quantification of inflammatory and epithelial cells, IL-6 and IL-8 concentrations in nasal lavage fluid. Twenty quitters (mean age: 51 years, 9 male) were assessed at baseline, 1 month, 3 months and 12 months after smoking cessation, and 13 smokers (mean age: 52 years, 6 male) were assessed at baseline and after 12 months. Clinicaltrials.gov: NCT02136550. RESULTS: Smokers and quitters showed similar demographic characteristics and morbidities. At baseline, all subjects showed impaired nasal mucociliary clearance (mean 17.6 min), although 63% and 85% of the quitters demonstrated significant nasal mucociliary clearance improvement at 1 month and 12 months, respectively. At 12 months, quitters also showed mucus sneeze clearability improvement (∼26%), an increased number of macrophages (2-fold) and no changes in mucus contact angle or cytokine concentrations. CONCLUSION: This study showed that smoking cessation induced early improvements in nasal mucociliary clearance independent of mucus properties and inflammation. Changes in mucus properties were observed after only 12 months of smoking cessation.
Descritores: Fumar/efeitos adversos
Abandono do Hábito de Fumar
Muco/química
-Fatores de Tempo
Monóxido de Carbono/análise
Fumar/metabolismo
Contagem de Células
Depuração Mucociliar
Estudos Longitudinais
Interleucina-8/metabolismo
Interleucina-6/metabolismo
Líquido da Lavagem Nasal/química
Cotinina/análise
Inflamação/patologia
Mucosa Nasal/patologia
Limites: Humanos
Masculino
Feminino
Adulto
Pessoa de Meia-Idade
Responsável: BR1.1 - BIREME


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Id: biblio-1008594
Autor: García, Apolinaria; Navarro, Karen; Sanhueza, Enrique; Pineda, Susana; Pastene, Edgar; Quezada, Manuel; Henríquez, Karem; Karlyshev, Andrey; Villena, Julio; González, Carlos.
Título: Characterization of Lactobacillus fermentum UCO-979C, a probiotic strain with a potent anti-Helicobacter pylori activity
Fonte: Electron. j. biotechnol;25:75-83, ene. 2017. tab, graf, ilus.
Idioma: en.
Projeto: INNOVA CHILE; . INNOVA BIO.
Resumo: Background: Helicobacter pylori is considered as the main risk factor in the development of gastric cancer. In the present study, we performed a detailed characterization of the probiotic properties and the anti-H. pylori activity of a previously isolated lactobacillus strain ­ Lactobacillus fermentum UCO-979C ­ obtained from human gut. Results: The strain tolerated pH 3.0; grew in the presence of 2% bile salts; produced lactic acid and hydrogen peroxide; aggregated in saline solution; showed high hydrophobicity; showed high adherence to glass; Caco-2 and gastric adenocarcinoma human cells (AGS) cells; showed an efficient colonization in Mongolian Gerbils; and potently inhibited the growth and urease activity of H. pylori strains. L. fermentum UCO-979C significantly inhibited H. pylori-induced IL-8 production in AGS cells and reduced the viability of H. pylori. With regard to innocuousness, the strain UCO-979C was susceptible to several antibiotics and did not produce histamine or beta-haemolysis in blood agar containing red blood cells from various origins. Conclusion: The results demonstrated that L. fermentum UCO-979C is a very good candidate as a probiotic for the protection of humans against H. pylori infections.
Descritores: Helicobacter pylori/efeitos dos fármacos
Infecções por Helicobacter/prevenção & controle
Probióticos/farmacologia
Lactobacillus fermentum/fisiologia
Antibacterianos/farmacologia
-Neoplasias Gástricas/prevenção & controle
Urease/antagonistas & inibidores
Interleucina-8/antagonistas & inibidores
Gerbillinae
Modelos Animais de Doenças
Interações Hidrofóbicas e Hidrofílicas
Limites: Humanos
Animais
Responsável: CL1.1 - Biblioteca Central



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